CN102863508A - Extraction technology of cerebral polypeptides - Google Patents
Extraction technology of cerebral polypeptides Download PDFInfo
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- CN102863508A CN102863508A CN 201110186739 CN201110186739A CN102863508A CN 102863508 A CN102863508 A CN 102863508A CN 201110186739 CN201110186739 CN 201110186739 CN 201110186739 A CN201110186739 A CN 201110186739A CN 102863508 A CN102863508 A CN 102863508A
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Abstract
The invention discloses an extraction technology of cerebral polypeptides, relating to the technical field of biochemical industry. The technology comprises the following steps: taking quarantined fresh pig brains and removing impurities, washing with purified water, then adding 4 times of purified water and conducting high speed cell breakage with a high-speed blender at twice; filtering with a filter cloth, and then homogenating by a homogenizer; adjusting the pH value, hydrolyzing by using complex enzyme to obtain low molecular weight small peptide, a mixture of free amino acid and nucleotide, heating up the enzymatic hydrolysate to 40-50 DEG C for removing active enzymes, distributing the enzyme removal liquid into containers, putting the containers in a refrigerator of -200 DEG C, freezing the containers for 12-24h and then taking the containers out, putting the containers at room temperature for thawing, taking the supernatant and centrifuging the supernatant with a centrifugal machine, removing fat, cell debris, residual denatured protein, nucleic acid and other large molecular impurities, taking the centrifugate and conducting unltrafiltration by using an unltrafiltration device. Compared with traditional process, the technology disclosed herein has the advantages of time saving, high efficiency, safety and environmental protection.
Description
Technical field
The present invention relates to the chemical industry biological technical field, be specifically related to a kind of brain polypeptide extraction process.
Background technology
Brain polypeptide is a kind of biotechnological formulation of good brain boosting and supplementing, the pig brain, because of its cost low, rich and easy to get, therefore be used for making the brain polypeptide series product, existing brain polypeptide extraction process is taked conventional multigelation technology, this technology length consuming time, virus is eliminated not thorough, dangerous environmental protection in the production process.
Summary of the invention
The purpose of this invention is to provide a kind of brain polypeptide extraction process, it has strengthened the biosafety control to raw material sources and production process, adopt the nano level cell breaking technology, nano level cell crashing ratio 〉=95%, improved about 5% than common process nano level cell crashing ratio, and shortened the production cycle, reduced energy consumption, adopt the prozyme of proteolytic enzyme and nuclease, with protein and the same one-step hydrolysis of nuclease, make in the product macromolecular substance degraded more thorough, and enriched the biological activity unit of product, adopt " single stage method " elimination virus, remove fat, cell debris, the impurity such as residual metaprotein and nucleic acid save time than traditional technology, efficiently, safety, the advantages such as environmental protection.
In order to solve the existing problem of background technology, the present invention takes following technical scheme: its technical process is: the fresh pig brain of the quarantine of learning from else's experience is removed manadesma, fat, brain centrum impurity, after using purified water to clean, add 4 times of amount purified water and use high speed stamp mill (20,000 rev/mins of rotating speeds) with its minute 2 high speed broken cells; Behind 20 order filter-cloth filterings, with clarifixator (more than the pressure 200Kg) homogenate; Add citric acid and adjust pH value, obtain the little peptide of lower molecular weight with combinative enzyme hydrolysis, total free aminoacids, mixture of ribonucleotides, this enzymolysis solution are heated to 40-50 ℃ except the enzyme of living, and will dezymotize liquid again and divide and be filled in the container, put as for-20
0The C freezer, take out after freezing 12-24 hours, put the room temperature thawing, get supernatant liquor and use the whizzer high speed centrifugation, remove fat, the macromole impurity extracting centrifugal liquids such as cell debris, residual metaprotein and nucleic acid are with ultrafiltration instrument (holding back with 20,000 molecular weight ultra-filtration membranes) ultrafiltration.
The present invention has following beneficial effect: it has strengthened the biosafety control to raw material sources and production process, adopt the nano level cell breaking technology, nano level cell crashing ratio 〉=95%, improved about 5% than common process nano level cell crashing ratio, and shortened the production cycle, reduced energy consumption, adopt the prozyme of proteolytic enzyme and nuclease, with protein and the same one-step hydrolysis of nuclease, make in the product macromolecular substance degraded more thorough, and the biological activity of having enriched product is first, adopt " single stage method " elimination virus, remove fat, cell debris, the impurity such as residual metaprotein and nucleic acid save time than traditional technology, efficiently, safety, the advantages such as environmental protection.
Embodiment:
This embodiment is taked following technical scheme: its technical process is: the fresh pig brain of the quarantine of learning from else's experience is removed manadesma, fat, brain centrum impurity, after using purified water to clean, add 4 times of amount purified water and use high speed stamp mill (20,000 rev/mins of rotating speeds) with its minute 2 high speed broken cells; Behind 20 order filter-cloth filterings, with clarifixator (more than the pressure 200Kg) homogenate; Add citric acid and adjust pH value, obtain the little peptide of lower molecular weight with combinative enzyme hydrolysis, total free aminoacids, mixture of ribonucleotides, this enzymolysis solution are heated to 40-50 ℃ except the enzyme of living, and will dezymotize liquid again and divide and be filled in the container, put as for-20
0The C freezer, take out after freezing 12-24 hours, put the room temperature thawing, get supernatant liquor and use the whizzer high speed centrifugation, remove fat, the macromole impurity extracting centrifugal liquids such as cell debris, residual metaprotein and nucleic acid are with ultrafiltration instrument (holding back with 20,000 molecular weight ultra-filtration membranes) ultrafiltration.
This embodiment has been strengthened the biosafety control to raw material sources and production process, adopt the nano level cell breaking technology, nano level cell crashing ratio 〉=95%, improved about 5% than common process nano level cell crashing ratio, and shortened the production cycle, reduced energy consumption, adopt the prozyme of proteolytic enzyme and nuclease, with protein and the same one-step hydrolysis of nuclease, make in the product macromolecular substance degraded more thorough, and the biological activity of having enriched product is first, adopt " single stage method " elimination virus, remove fat, cell debris, the impurity such as residual metaprotein and nucleic acid save time than traditional technology, efficiently, safety, the advantages such as environmental protection.
Claims (3)
1. brain polypeptide extraction process is characterized in that its technical process is: the fresh pig brain of the quarantine of learning from else's experience is removed manadesma, fat, brain centrum impurity, use purified water to clean after, add 4 times of amount purified water with the high speed stamp mill with its minute 2 high speed broken cells; Behind 20 order filter-cloth filterings, use the clarifixator homogenate; Add citric acid and adjust pH value, obtain the little peptide of lower molecular weight with combinative enzyme hydrolysis, total free aminoacids, mixture of ribonucleotides, this enzymolysis solution are heated to 40-50 ℃ except the enzyme of living, and will dezymotize liquid again and divide and be filled in the container, put as for-20
0The C freezer takes out after freezing 12-24 hours, puts the room temperature thawing, gets supernatant liquor and uses the whizzer high speed centrifugation, removes fat, and the macromole impurity extracting centrifugal liquids such as cell debris, residual metaprotein and nucleic acid are with the ultrafiltration of ultrafiltration instrument.
2. a kind of brain polypeptide extraction process according to claim 1, the rotating speed that it is characterized in that described stamp mill is 20,000 rev/mins.
3. a kind of brain polypeptide extraction process according to claim 1 is characterized in that described ultrafiltration instrument holds back ultrafiltration with 20,000 molecular weight ultra-filtration membranes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110186739 CN102863508A (en) | 2011-07-05 | 2011-07-05 | Extraction technology of cerebral polypeptides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110186739 CN102863508A (en) | 2011-07-05 | 2011-07-05 | Extraction technology of cerebral polypeptides |
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| Publication Number | Publication Date |
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| CN102863508A true CN102863508A (en) | 2013-01-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201110186739 Pending CN102863508A (en) | 2011-07-05 | 2011-07-05 | Extraction technology of cerebral polypeptides |
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| CN (1) | CN102863508A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388509A (en) * | 2014-10-27 | 2015-03-04 | 合肥平光制药有限公司 | Preparation method of hepatocyte growth-promoting factors employing ultrasonic treatment |
| CN105815663A (en) * | 2016-05-24 | 2016-08-03 | 珍奥集团股份有限公司 | Method for extracting nucleic acid raw material from natural foods |
| CN109452653A (en) * | 2018-10-22 | 2019-03-12 | 广东羲准生物科技有限公司 | Join brain peptide composition and its application |
| CN109806279A (en) * | 2017-11-17 | 2019-05-28 | 江西康宝医药生物科技有限公司 | A kind of Medulla sus domestica extract fusion Chinese medicine improves the oral solution of memory |
| CN111329071A (en) * | 2020-03-13 | 2020-06-26 | 江西康宝医药生物科技有限公司 | Preparation process of brain polypeptide |
-
2011
- 2011-07-05 CN CN 201110186739 patent/CN102863508A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388509A (en) * | 2014-10-27 | 2015-03-04 | 合肥平光制药有限公司 | Preparation method of hepatocyte growth-promoting factors employing ultrasonic treatment |
| CN105815663A (en) * | 2016-05-24 | 2016-08-03 | 珍奥集团股份有限公司 | Method for extracting nucleic acid raw material from natural foods |
| CN109806279A (en) * | 2017-11-17 | 2019-05-28 | 江西康宝医药生物科技有限公司 | A kind of Medulla sus domestica extract fusion Chinese medicine improves the oral solution of memory |
| CN109452653A (en) * | 2018-10-22 | 2019-03-12 | 广东羲准生物科技有限公司 | Join brain peptide composition and its application |
| CN111329071A (en) * | 2020-03-13 | 2020-06-26 | 江西康宝医药生物科技有限公司 | Preparation process of brain polypeptide |
| CN111329071B (en) * | 2020-03-13 | 2021-08-13 | 江西康宝医药生物科技有限公司 | Preparation process of brain polypeptide |
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Application publication date: 20130109 |