CN102978268A - Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method - Google Patents
Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method Download PDFInfo
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- CN102978268A CN102978268A CN2012105711443A CN201210571144A CN102978268A CN 102978268 A CN102978268 A CN 102978268A CN 2012105711443 A CN2012105711443 A CN 2012105711443A CN 201210571144 A CN201210571144 A CN 201210571144A CN 102978268 A CN102978268 A CN 102978268A
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Abstract
The invention relates to the field of production of high-purity egg albumin polypeptide, and especially discloses a method for preparing egg albumin polypeptide from egg albumin powder by an enzymic method. According to the method for preparing egg albumin polypeptide from egg albumin powder by an enzymic method, the egg albumin powder is used as raw material; and the method provided by the invention is characterized by comprising the following steps of: dissolving the egg albumin powder in deionized water, adding a papain solution for hydrolysis, then, heating up the solution to be boiling, preserving heat and deactivating enzyme; executing membrane filtration to collect filtrate from the solution treated by enzyme deactivation; concentrating the filtrate in a vacuum low-temperature triple effect concentrating pan, and then, adding the active carbon, heating up, preserving heat and executing frame filtering, and carrying out spray drying for the filtrate to get the finished product. The method provided by the invention is simple in steps, low in production cost, exhaustive in enzymolysis, short in reaction time, easy to filter, high in peptide yield and capable of greatly improving the technological content and the quality of products, and being used and popularized widely.
Description
(1) technical field
The present invention relates to high purity Protalbinic acid polypeptide production field, particularly a kind of from the ovum protein powder method of ovum by enzymatical process albumin polypeptide.
(2) background technology
Protein peptide is a kind of biologically active substance, is that albumen is through the prepared mixing oligopeptide with some biological function of the methods such as protease hydrolysis or microbial fermentation.From chemical terms, protein peptide then is that 20 natural amino acids form with difference and the general name of the different peptide classes of the linearity from the dipeptides to the complexity of arrangement mode formation, ring structure in the protein.Biological test shows that the protein that body is taken in absorbs with the low molecular weight peptide form mostly after gastral enzyme effect, the ratio that absorbs with the total free aminoacids form is very little.Further test disclosed again the digestion of low molecular weight peptide specific ionization amino acid faster, absorb manyly, above-mentioned result of study all biological value and the nutritive value specific ionization amino acid of surperficial low molecular weight peptide is higher.
At present, the Protalbinic acid polypeptide generally obtains by the direct enzymolysis of ovum protein liquid, and the production process difficulty is large, the time is long, should not filter, and it is low to obtain the peptide rate.
(3) summary of the invention
The present invention is in order to remedy the deficiencies in the prior art, the method for ovum by enzymatical process albumin polypeptide from the ovum protein powder that provides that a kind of step is simple, production cost is low.
The present invention is achieved through the following technical solutions:
A kind of from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, take the ovum protein powder as raw material, it is characterized in that: the ovum protein powder is dissolved in the deionized water, add papoid solution, be the hydrolysis in 8 hours of 7 ± 1 times stirring reactions in 40 ± 1 ℃, pH, then be warming up to after the boiling insulation 5min enzyme that goes out; The solution that will go out behind the enzyme is collected filtrate by membrane filtration; Place vacuum and low temperature triple effect concentrating pan concentrated filtrate, then add the gac of concentrated solution quality 8 ~ 10%, be warming up to 75 ℃ after insulation carry out Plate Filtration, filtrate is carried out spraying drying obtains finished product.
The present invention adopts this natural active substance of ovum protein powder, and its molecular weight is larger, is difficult for directly absorbed by the body, be hydrolyzed into a kind of polypeptide and amino acid whose Protalbinic acid peptide product that easily is absorbed by the body that contain, cost is low, has no side effect, but as the nutritive food widespread use.
More excellent scheme of the present invention is:
The quality proportioning of described ovum protein powder and deionized water is 1:2, and papoid solution is that every 1g papoid adds the 1kg deionized water.
The film that adopts during described membrane filtration is 0.3 μ m aperture.
The mass concentration of described concentrated solution is 20%.
Under 75 ℃, be incubated 0.2 hour before the described Plate Filtration.
Particle is crossed 60 mesh sieves after the described spraying drying.
Its protein content of product of the present invention is more than 85%, the molecular weight of 90% Protalbinic acid polypeptide is below 1000 Dalton, also be designed into simultaneously the limited enzymatic hydrolysis of ovum protein pruinescence, produce the Protalbinic acid polypeptide, it is directly edible to can be used as the nutrient protein supplement, also can be used as the function raw material of clinical nutrition product, protective foods, sport video and makeup, be worth for improving the ovum egg product, the product of production high value has found a kind of new approach.
Step of the present invention is simple, and production cost is low, thorough enzymolysis, and the reaction times is short, very easily filters, and it is high to obtain the peptide rate, has greatly improved scientific and technological content and the quality of product, uses wide popularization and application.
(4) embodiment
Embodiment:
Its processing step is:
Ovum protein powder → add water adjust pH → add enzymic hydrolysis → intensification enzyme → filtration → vacuum concentration → cryodrying → Protalbinic acid polypeptide that goes out of sizing mixing → heat.
Its concrete operation step is:
(1) 5kg ovum protein powder is dissolved in the 10kg deionized water, and constantly stirs;
(2) get papoid 10g(enzyme activity 1,000,000 u/g), add the 10kg deionized water, add after the dissolving in the feed liquid, stirring fully mixes; Temperature stirs in this process, and temperature is controlled at positive and negative 1 degree of 40 degree, and PH was 7 ± 1 reactions 8 hours;
(3) hydrolysis stopped reaction after 8 hours, enzymolysis is complete, is warming up to simultaneously boiling state and keeps 5 minutes, fully deactivation;
(4) go out and use the 0.3um membrane filtration behind the enzyme, elimination is the albumin of enzymolysis, collects filtrate 10000 ~ 11000ml;
(5) filtrate enters vacuum and low temperature triple effect concentrating pan and concentrates concentration 20% discharging 2500 ~ 3000ml;
(6) add 8-10% activated carbon 400-500g, Plate Filtration is carried out in the 75 ℃ of insulations 0.2 hour that heat up;
(7) feed liquid is carried out spraying drying, cross 60 mesh sieves, product yield 45-50%; The laminated bag packing.
Claims (6)
1. the method for an ovum by enzymatical process albumin polypeptide from the ovum protein powder, take the ovum protein powder as raw material, it is characterized in that: the ovum protein powder is dissolved in the deionized water, add papoid solution, be the hydrolysis in 8 hours of 7 ± 1 times stirring reactions in 40 ± 1 ℃, pH, then be warming up to after the boiling insulation 5min enzyme that goes out; The solution that will go out behind the enzyme is collected filtrate by membrane filtration; Place vacuum and low temperature triple effect concentrating pan concentrated filtrate, then add the gac of concentrated solution quality 8 ~ 10%, be warming up to 75 ℃ after insulation carry out Plate Filtration, filtrate is carried out spraying drying obtains finished product.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: the quality proportioning of described ovum protein powder and deionized water is 1:2, papoid solution is that every 1g papoid adds the 1kg deionized water.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: the film that adopts during described membrane filtration is 0.3 μ m aperture.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: the mass concentration of described concentrated solution is 20%.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: before the described Plate Filtration 75 ℃ of lower insulations 0.2 hour.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: particle is crossed 60 mesh sieves after the described spraying drying.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012105711443A CN102978268A (en) | 2012-12-26 | 2012-12-26 | Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method |
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| CN2012105711443A CN102978268A (en) | 2012-12-26 | 2012-12-26 | Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104480175A (en) * | 2014-07-22 | 2015-04-01 | 上海荣神生物化学有限公司 | Enzymolysis method of ovalbumin and application of enzymolysis products |
| CN109091409A (en) * | 2018-10-25 | 2018-12-28 | 福建农林大学 | A kind of preparation method of passionflower anthocyanidin and ovalbumin peptide facial mask dry powder |
| CN110771902A (en) * | 2019-11-21 | 2020-02-11 | 广东肽世家生物科技有限公司 | Composite polypeptide powder and application thereof |
| CN111631337A (en) * | 2020-05-30 | 2020-09-08 | 泉州玺阅日化有限公司 | Health effervescent tablet containing animal albumin and preparation method thereof |
| CN112250758A (en) * | 2020-09-21 | 2021-01-22 | 杨志伟 | Albumin stock solution, preparation method and application thereof |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CN113462559A (en) * | 2021-07-26 | 2021-10-01 | 吴艳 | Promotion preparation system suitable for albumin polypeptide |
| US11160299B2 (en) | 2019-07-11 | 2021-11-02 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US11279748B2 (en) | 2014-11-11 | 2022-03-22 | Clara Foods Co. | Recombinant animal-free food compositions and methods of making them |
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| CN1101377A (en) * | 1993-10-07 | 1995-04-12 | 上海益人生化技术开发有限公司 | Enzymolytic egg albumen solution as health-care liquid |
| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN101096697A (en) * | 2007-07-06 | 2008-01-02 | 山东天久生物技术有限公司 | Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process |
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2012
- 2012-12-26 CN CN2012105711443A patent/CN102978268A/en active Pending
Patent Citations (3)
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| CN1101377A (en) * | 1993-10-07 | 1995-04-12 | 上海益人生化技术开发有限公司 | Enzymolytic egg albumen solution as health-care liquid |
| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN101096697A (en) * | 2007-07-06 | 2008-01-02 | 山东天久生物技术有限公司 | Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104480175B (en) * | 2014-07-22 | 2018-02-27 | 上海荣神生物化学有限公司 | A kind of method of enzyme-hydrolysis albumen and the application of enzymolysis product |
| CN104480175A (en) * | 2014-07-22 | 2015-04-01 | 上海荣神生物化学有限公司 | Enzymolysis method of ovalbumin and application of enzymolysis products |
| US11518797B2 (en) | 2014-11-11 | 2022-12-06 | Clara Foods Co. | Methods and compositions for egg white protein production |
| US11279748B2 (en) | 2014-11-11 | 2022-03-22 | Clara Foods Co. | Recombinant animal-free food compositions and methods of making them |
| CN109091409A (en) * | 2018-10-25 | 2018-12-28 | 福建农林大学 | A kind of preparation method of passionflower anthocyanidin and ovalbumin peptide facial mask dry powder |
| CN109091409B (en) * | 2018-10-25 | 2021-09-21 | 福建农林大学 | Preparation method of passion flower anthocyanin and egg albumin peptide mask dry powder |
| US11160299B2 (en) | 2019-07-11 | 2021-11-02 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US11974592B1 (en) | 2019-07-11 | 2024-05-07 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US11800887B2 (en) | 2019-07-11 | 2023-10-31 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US11142754B2 (en) | 2019-08-07 | 2021-10-12 | Clara Foods Co. | Compositions comprising digestive enzymes |
| US11649445B2 (en) | 2019-08-07 | 2023-05-16 | Clara Foods Co. | Compositions comprising digestive enzymes |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CN110771902A (en) * | 2019-11-21 | 2020-02-11 | 广东肽世家生物科技有限公司 | Composite polypeptide powder and application thereof |
| CN111631337A (en) * | 2020-05-30 | 2020-09-08 | 泉州玺阅日化有限公司 | Health effervescent tablet containing animal albumin and preparation method thereof |
| CN112250758A (en) * | 2020-09-21 | 2021-01-22 | 杨志伟 | Albumin stock solution, preparation method and application thereof |
| CN113462559A (en) * | 2021-07-26 | 2021-10-01 | 吴艳 | Promotion preparation system suitable for albumin polypeptide |
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Application publication date: 20130320 |