CN106282144A - A kind of bromelain production method - Google Patents
A kind of bromelain production method Download PDFInfo
- Publication number
- CN106282144A CN106282144A CN201610843427.7A CN201610843427A CN106282144A CN 106282144 A CN106282144 A CN 106282144A CN 201610843427 A CN201610843427 A CN 201610843427A CN 106282144 A CN106282144 A CN 106282144A
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- China
- Prior art keywords
- bromelain
- production method
- juice
- microfiltration
- ceramic membrane
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22004—Bromelain (3.4.22.4)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses a kind of bromelain production method, comprise the following steps: step one, cleaning treatment；Step 2, pulverizing；Step 3, squeeze the juice；Step 4, two-stage centrifugal；Step 5, microfiltration of ceramic membrane；Step 6, ultrafiltration concentration；Step 7, be dried；The present invention is by carrying out two-stage centrifugal process to the pineapple juice squeezed, improve the purity of Clarification of Pineapple Juice, decrease microfiltration pressure, by using microfiltration of ceramic membrane to process, the height not only realized under the physical state to viable bacteria body retains, concurrently separate out the enzyme downstream clear liquid that clarity is the highest, reduce the production load of downstream concentration technique, it is concentrated by ultrafiltration by employing, eliminate partial pigment and foreign protein, improve product quality and stability, be concentrated by ultrafiltration simultaneously and carry out at normal temperatures, enzyme is lived and is not lost, and improves the yield of bromelain.
Description
Technical field
The present invention designs a kind of pharmaceutical mould production method, particularly relates to the production method of a kind of bromelain.
Background technology
Bromelain is a kind of to come at extraction from pineapple sarcocarp, stem, leaf, through refining, purify, concentrating, after lyophilization
A kind of natural plant protein enzyme arrived, has the strongest decomposition fibrin and the ability of blood clot, can also promote medicine simultaneously
Absorb and the growth of suppression tumor cell.
At present, the main production process of bromelain has physical extracting method and chemical manufacturing process, along with membrane technology
Development, concentrate membrance separation and extract bromelain and have been obtained for extensively applying, but contain due to pineapple juice in separation process
Impurity is more, and required booster pump pressure is relatively big, and is easily damaged concentration film, reduces the service life concentrating film.
Summary of the invention
It is an object of the invention to provide a kind of can be with the method for high efficiency extraction bromelain, it is ensured that the bromelain of extraction
The quality of enzyme and enzymatic activity.
The production method of a kind of bromelain of technical solution of the present invention, comprises the following steps:
Step one, cleaning treatment, by peeling pineapple, place stand-by after cleaning up；
Step 2, pulverizing, put into the Fructus Ananadis comosi of wash clean in pulverizer the speed with 200r/min and be stirred, obtain powder
Fructus Ananadis comosi；
Step 3, squeezing the juice, the Fructus Ananadis comosi after pulverizing is put juice extractor into and prepares pineapple juice suspension；
Step 4, two-stage centrifugal, put pineapple juice suspension centrifuge into and carry out first-stage centrifugal process, after standing a period of time again
Secondary carry out secondary centrifuging process, go the removal of impurity, obtain the pineapple juice of clarification；
Step 5, microfiltration of ceramic membrane, be passed through Clarification of Pineapple Juice ceramic membrane separation device 0.2mpa and carry out micro-filtration process, obtain
The enzyme downstream stillness of night；
Step 6, ultrafiltration concentration, take enzyme downstream clear liquid dense under 0.3mpa pressure by charging pump feeding ultrafilter membrane concentrator
Contracting processes, and removes partial pigment and foreign protein, obtains bromelain concentrated solution；
Step 7, be dried, concentrated solution is carried out vacuum drying treatment, obtains bromelain.
Preferably, in described step 2, the mixing speed of pulverizer is 200r/min.
Preferably, in described step 4, centrifuge speed is 220 r/min, each centrifugation time 30 minutes.
Preferably, in described step 6, ultrafilter membrane selects Merlon, and molecular cut off is 25000.
Preferably, the pressure in described step 5 and step 6 is provided by booster pump.
Preferably, in described step 5, microfiltration of ceramic membrane equipment is connected by pipeline with concentrator in step 6.
The technology of the present invention beneficial effect:
The production method of a kind of bromelain of the present invention, by the pineapple juice squeezed is carried out two-stage centrifugal process, improves
The purity of Clarification of Pineapple Juice, decreases microfiltration pressure, by using microfiltration of ceramic membrane to process, not only realizes viable bacteria body
Height under physical state retains, and concurrently separates out the enzyme downstream clear liquid that clarity is the highest, reduces the production of downstream concentration technique
Load, is concentrated by ultrafiltration by employing, eliminates partial pigment and foreign protein, improves product quality and stability, simultaneously ultrafiltration
Concentrating and carry out at normal temperatures, enzyme is lived and is not lost, and improves the yield of bromelain.
Accompanying drawing explanation
Fig. 1 is the process chart of the production method of a kind of bromelain of the present invention.
Detailed description of the invention
For ease of skilled artisan understands that technical solution of the present invention, in conjunction with Figure of description, technical solution of the present invention is done
Further instruction.
Embodiment:
As it is shown in figure 1, the production method of a kind of bromelain of the present invention, comprise the following steps: at the cleaning of Fructus Ananadis comosi raw material
Manage, pulverize, squeeze the juice, two-stage centrifugal, microfiltration of ceramic membrane, ultrafiltration concentration, be dried, prepare finished product.
Specifically: Fructus Ananadis comosi raw material is removed the peel, after cleaning up in putting rinse bath into, with pulverizer with the stirring of 200r/min
Speed carries out pulverization process to Fructus Ananadis comosi；Fructus Ananadis comosi after pulverizing by conveyer belt be transported to juice extractor carry out squeezing the juice obtain Fructus Ananadis comosi suspend
Liquid；Fructus Ananadis comosi suspension is put into and after centrifuge is centrifuged 30 minutes with the rotating speed of 220 r/min, obtains first centrifugal clarification Fructus Ananadis comosi
Juice, is placed again into centrifuge after Clarification of Pineapple Juice is stood 5 minutes and carries out secondary centrifuging process with identical speed and time,
To final Clarification of Pineapple Juice；Clarification of Pineapple Juice is passed through microfiltration of ceramic membrane equipment, starts booster pump and carry out micro-mistake with 0.2mpa
Filter processes, and obtains, after removing partial pigment and foreign protein, the enzyme downstream clear liquid that clarity is the highest；Gained enzyme downstream clear liquid directly leads to
Cross Pipeline transport and carry out concentrating and separating to film device is concentrated by ultrafiltration, obtain bromelain concentrated solution；Bromelain is concentrated
Liquid is put into after vacuum drying oven is dried 10 hours with 60 DEG C of temperature and is obtained solid-state bromelain.
Invention is exemplarily described by technical solution of the present invention above in conjunction with accompanying drawing, it is clear that the present invention implements
It is not subject to the restrictions described above, as long as the various unsubstantialities that method is conceived and technical scheme is carried out that have employed the present invention change
Enter, or the most improved design by invention and technical scheme directly apply to other occasion, all in protection scope of the present invention
Within.
Claims (6)
1. a bromelain production method, it is characterised in that comprise the following steps:
Step one, cleaning treatment, by peeling pineapple, place stand-by after cleaning up；
Step 2, pulverizing, put into the Fructus Ananadis comosi of wash clean in pulverizer the speed with 200r/min and be stirred, obtain powder
Fructus Ananadis comosi；
Step 3, squeezing the juice, the Fructus Ananadis comosi after pulverizing is put juice extractor into and prepares pineapple juice suspension；
Step 4, two-stage centrifugal, put pineapple juice suspension centrifuge into and carry out first-stage centrifugal process, after standing a period of time again
Secondary carry out secondary centrifuging process, go the removal of impurity, obtain the pineapple juice of clarification；
Step 5, microfiltration of ceramic membrane, be passed through Clarification of Pineapple Juice ceramic membrane separation device 0.2mpa and carry out micro-filtration process, obtain
The enzyme downstream stillness of night；
Step 6, ultrafiltration concentration, take enzyme downstream clear liquid dense under 0.3mpa pressure by charging pump feeding ultrafilter membrane concentrator
Contracting processes, and removes partial pigment and foreign protein, obtains bromelain concentrated solution；
Step 7, be dried, concentrated solution is carried out vacuum drying treatment, obtains bromelain.
A kind of bromelain production method the most according to claim 1, it is characterised in that in described step 2, pulverize
The mixing speed of machine is 200r/min.
A kind of bromelain production method the most according to claim 1, it is characterised in that in described step 4, centrifugal
Machine rotating speed is 220 r/min, each centrifugation time 30 minutes.
A kind of bromelain production method the most according to claim 1, it is characterised in that in described step 6, ultrafiltration
Film selects Merlon, and molecular cut off is 25000.
A kind of bromelain production method the most according to claim 1, it is characterised in that described step 5 and step 6
In pressure provided by booster pump.
A kind of bromelain production method the most according to claim 1, it is characterised in that ceramic membrane in described step 5
Microfiltration equipment is connected by pipeline with concentrator in step 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610843427.7A CN106282144A (en) | 2016-09-23 | 2016-09-23 | A kind of bromelain production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610843427.7A CN106282144A (en) | 2016-09-23 | 2016-09-23 | A kind of bromelain production method |
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| Publication Number | Publication Date |
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| CN106282144A true CN106282144A (en) | 2017-01-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610843427.7A Pending CN106282144A (en) | 2016-09-23 | 2016-09-23 | A kind of bromelain production method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267488A (en) * | 2017-08-20 | 2017-10-20 | 合肥信达膜科技有限公司 | A kind of efficient bromelain preparation method |
| CN107475226A (en) * | 2017-09-19 | 2017-12-15 | 青岛农业大学 | A kind of new Bromenzyme and preparation method thereof |
| CN108525536A (en) * | 2018-03-22 | 2018-09-14 | 四川美酷投资管理有限公司 | A kind of Intelligent Production System of makeup remover |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319208A (en) * | 2008-04-08 | 2008-12-10 | 徐闻县美仑生物制品有限公司 | Method for preparation of bromelin |
| CN102071180A (en) * | 2009-11-23 | 2011-05-25 | 湖州来色生物基因工程有限公司 | New process for preparing pineapple bromelain |
-
2016
- 2016-09-23 CN CN201610843427.7A patent/CN106282144A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319208A (en) * | 2008-04-08 | 2008-12-10 | 徐闻县美仑生物制品有限公司 | Method for preparation of bromelin |
| CN102071180A (en) * | 2009-11-23 | 2011-05-25 | 湖州来色生物基因工程有限公司 | New process for preparing pineapple bromelain |
Non-Patent Citations (2)
| Title |
|---|
| 梁宏宇: "菠萝蛋白酶提取方法的研究", 《中国优秀硕士学位论文全文数据库（电子期刊）》 * |
| 韩志萍等: "超滤分离纯化菠萝蛋白酶的研究", 《食品工业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267488A (en) * | 2017-08-20 | 2017-10-20 | 合肥信达膜科技有限公司 | A kind of efficient bromelain preparation method |
| CN107475226A (en) * | 2017-09-19 | 2017-12-15 | 青岛农业大学 | A kind of new Bromenzyme and preparation method thereof |
| CN108525536A (en) * | 2018-03-22 | 2018-09-14 | 四川美酷投资管理有限公司 | A kind of Intelligent Production System of makeup remover |
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Application publication date: 20170104 |
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