CN105815663A - Method for extracting nucleic acid raw material from natural foods - Google Patents
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- CN105815663A CN105815663A CN201610348190.5A CN201610348190A CN105815663A CN 105815663 A CN105815663 A CN 105815663A CN 201610348190 A CN201610348190 A CN 201610348190A CN 105815663 A CN105815663 A CN 105815663A
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- nucleic acid
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- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 79
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 79
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 79
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000002994 raw material Substances 0.000 title claims abstract description 19
- 235000013305 food Nutrition 0.000 title abstract description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 42
- 239000000243 solution Substances 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000011780 sodium chloride Substances 0.000 claims abstract description 21
- 239000012153 distilled water Substances 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 11
- 238000003828 vacuum filtration Methods 0.000 claims abstract description 11
- 238000007710 freezing Methods 0.000 claims abstract description 8
- 230000008014 freezing Effects 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 238000001556 precipitation Methods 0.000 claims abstract description 8
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 5
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 5
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 5
- 238000010790 dilution Methods 0.000 claims abstract description 5
- 239000012895 dilution Substances 0.000 claims abstract description 5
- 238000000605 extraction Methods 0.000 claims description 24
- 210000000582 semen Anatomy 0.000 claims description 24
- 239000000463 material Substances 0.000 claims description 17
- 239000003085 diluting agent Substances 0.000 claims description 13
- 238000010257 thawing Methods 0.000 claims description 12
- 235000020795 whole food diet Nutrition 0.000 claims description 11
- 240000007594 Oryza sativa Species 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- 238000007865 diluting Methods 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 240000006394 Sorghum bicolor Species 0.000 claims description 4
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 4
- 239000000084 colloidal system Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 abstract description 9
- 239000000047 product Substances 0.000 abstract description 7
- 230000002255 enzymatic effect Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000003960 organic solvent Substances 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 2
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 238000000227 grinding Methods 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 239000000543 intermediate Substances 0.000 abstract 1
- 238000001728 nano-filtration Methods 0.000 abstract 1
- 239000006072 paste Substances 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 7
- 239000000796 flavoring agent Substances 0.000 description 6
- 239000002253 acid Substances 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 230000000050 nutritive effect Effects 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 239000004375 Dextrin Substances 0.000 description 3
- 229920001353 Dextrin Polymers 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 235000019425 dextrin Nutrition 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 235000013355 food flavoring agent Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 3
- 239000002562 thickening agent Substances 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011344 liquid material Substances 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B15/00—Organic phosphatic fertilisers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for extracting a nucleic acid raw material from natural foods .The method comprises the steps that the natural foods are ground into powder or paste, distilled water is added, and repeated freezing and unfreezing are conducted at a low speed; water is added to freezing liquid to adjust the pH value to be 8 to 10; grinding is conducted for 3 min to 5 min, the pH value is adjusted to be 6 to 7 with a homogenate, centrifugation is conducted, and a nucleic acid extracting solution is obtained; neutral protease is added, reacting is conducted at 45 DEG C to 55 DEG C for 4 h to 6 h, and an enzymatic hydrolysate is obtained; sodium chloride is added to the enzymatic hydrolysate, centrifugation and precipitation collection are conduced, the distilled water is added for dilution, vacuum filtration is conducted with a nanofiltration membrane to remove sodium chloride, drying is conducted, and the nucleic acid raw material is obtained .According to the method for extracting the nucleic acid raw material from the natural foods, no organic solvent is adopted in the whole extracting process of nucleic acid, and the food safety of the product is guaranteed; the method is simple, the production cycle is short, the cost is low, resources and energy are fully utilized, intermediates are fully utilized, resource waste is reduced, environmental pollution is reduced, the nucleic acid products are diversified, and the market resources are enriched.
Description
Technical field
The present invention relates to a kind of method extracting nucleic acid raw material, particularly relate to from wholefood, extract nucleic acid former
The method of material.
Background technology
Ribonucleic acid (RNA) and DNA (deoxyribonucleic acid) (DNA) is included in the generally academic definition of nucleic acid, this
Two kinds of nucleic acid are all the material bases of biological heredity function, and the most tellurian all biologies are all containing core
Acid.In order to transform species, creating a class and be more beneficial for the organism of human survival, genetic engineering is in the whole world
Being paid attention to by scientist, engineered core technology is the restructuring of hereditary material nucleic acid, converts, transduces
And the mensuration that puts in order and structural change, many work be unable to do without the extracting of nucleic acid, purification, but this side
The research in face simply operates work in gram the most subtly, is not particularly suited for the extensive of nucleic acid
Industrialized production.
Nucleic acid just has higher nutritive value, food amplifying nucleic acid to have an offer immunocompetence, oxidation resistance,
Can promote that cell regeneration and reparation etc. act on.Nucleic acid raw material mainly extracts from plant, microorganism and obtains, but
Also there is no to be applicable to the nucleic acid raw material extracting method of large-scale industrial production.Such as draft and xylophyta
Though their quantity is big, but nucleic acid content is the lowest, it is impossible to as the raw material of nucleic acid extraction.At microbiology class
In Qun, some antibacterials and viral nucleic acid content are up to more than 50%, but are intended to, by cultivating, obtain this in a large number
Bacterioid or virus are extremely difficult, or Financial cost loses more than gain.
Currently also have from wholefood, as beans, greengrocery etc. are extracted nucleic acid, but according to its source difference,
Nucleic acid may often be such that and jointly exists with other materials multiple as a mixture, as protein, lipid and other
Composition.So for the high nucleic acid raw material of purity, needing to use loaded down with trivial details extraction and purge process, using more
Solvent, adsorbent etc., this cost not only increased, too increase the insecurity used as food.
Further, there is resource utilization is low, nucleic acid sterling purity is relatively low phenomenon and become in existing nucleic acid extraction technology
Gesture.
Summary of the invention
Loaded down with trivial details for technique when extracting at present nucleic acid raw material from food, cost is high, safety is low, resource
Utilization rate and low etc. the problem of product purity, the present invention provides a kind of side extracting nucleic acid from wholefood
Method.
Technical scheme is as follows:
A kind of method extracting nucleic acid from wholefood, comprises the steps:
(1) wholefood is ground to form powder or pastel；
(2) distilled water of the 5-25% of powder or pastel quality, slow freezing are added；
(3) frozen material thaws in 65-80 DEG C of water-bath；
(4) thawing solution that step (2) obtains adds water dilution, tune pH value to 8-10；
(5) the diluent colloid mill that step (3) obtains grinds 3-5min, obtains homogeneous liquid；
(6) homogeneous liquid adjusts pH value to 6-7, is centrifuged, and collects supernatant, obtains nucleic acid extraction liquid；
(7) nucleic acid extraction liquid adds neutral protease, at 45-55 DEG C, react 4-6h, obtain enzymolysis solution；
(8) enzymolysis solution adds sodium chloride, centrifugal, collect precipitation, add distilled water diluting, use NF membrane
Vacuum filtration removes sodium chloride, is dried, obtains nucleic acid raw material.
In technique scheme, it is preferable that step (2)-(3) are repeated 2-3 time, will frozen material solution
Be frozen into aqueous after, then carry out slow freezing, then thaw, 2-3 time the most repeatedly, this operation is conducive to broken
Cell wall, makes nucleic acid be fully dissolved out.
Further, in step (4), described thawing solution is 1:5-10 with the mass ratio of the water of addition.
Further, the centrifugal condition described in step (6) is: at 4 DEG C, with 3000-5000
Centrifugal 10-20min under rpm.
Further, in step (8), enzymolysis solution adds sodium chloride to making sodium chloride in enzymolysis solution
Weight/mass percentage composition be 8-12%.
Further, in step (8), described precipitation and the mass ratio of the water of addition are 1:1-2.
Further, described wholefood is Semen Phaseoli, Semen phaseoli radiati, Semen Viciae fabae, Semen Pisi sativi, sorghum rice, Semen Maydis, little
Rice, Fructus Hordei Vulgaris or Semen Tritici aestivi.
Further, in step (8), nucleic acid solution is dried condition and is: use drying under reduced pressure case,
Being dried 8-12h under 0.07-0.09Mpa, material thickness is 0.5-1.5cm.
In the inventive solutions, in step (8), in precipitation add distilled water diluting obtain dilute
Release liquid, after removing sodium chloride, aminoacid with NF membrane vacuum filtration, be equipped with thickening agent, sweeting agent etc. permissible
Make natural acid nutritional solution；The raw materials such as appropriate sweeting agent and dextrin, drying, granulation work can also be equipped with
Skill can make nutritive nucleic acid electuary；Diluent NF membrane vacuum filtration can also be removed sodium chloride, filter
Containing nucleic acid and aminoacid in liquid, local flavor flavoring agent etc. can be made.Separately, obtain in step (8) is heavy
Shallow lake can use as nucleic acid organic feed or chemical fertilizer.
In the technique scheme of the present invention, it is more than 80 mesh that wholefood preferably grinds to form granularity
Powder or pastel.
Beneficial effects of the present invention: based on when extracting nucleic acid in natural goods at present, in order to improve carrying of nucleic acid
Take efficiency, the present situations using organic solvent more, the present invention uses the method that freezing crushing combines physical pulverization,
To accelerate the cracking of cell and nucleoprotein and to strengthen the cutting to nucleic acid molecule, nucleic acid is made to be fully dissolved out,
Ensureing the high extraction of nucleic acid, the method makes full use of the existing energy, saves the energy, and improves extraction ratio.
Using enzymatic isolation method decomposing protein, make the abundant enzymolysis of protein, nucleic acid purity improves 5%-10%.This
Bright during the extraction of whole nucleic acid, do not use any organic solvent, it is ensured that the food safety of product.This
Bright, technique is simple, with short production cycle, low cost, and harmful liquid discharge is few, and environmental protection makes full use of resource
And the energy, make intermediate make full use of, reduce the wasting of resources, reduce environmental pollution, make nucleic acid product various
Change, abundant market resource.The method using the present invention, the water content of the nucleic acid raw material finally given is 5%
Hereinafter, nucleic acid content is more than 90%.
Detailed description of the invention
Following non-limiting example can make those of ordinary skill in the art that the present invention is more fully understood,
But limit the present invention never in any form.In following embodiment, if no special instructions, the experiment side used
Method is conventional method, and agents useful for same etc. all can chemically or biological reagent company buys.
Embodiment 1
From Semen sojae atricolor, Semen Phaseoli, Semen vignae sinensis, Semen phaseoli radiati, extract nucleic acid, comprise the steps:
(1) taking Semen Phaseoli (or Semen phaseoli radiati, Semen Viciae fabae, Semen Pisi sativi) and grind to form powder, granularity is 80-100 mesh,
Add its quality 25% distilled water；
(2) freezing 10 hours at-20 DEG C, keep material cooling rate 2 DEG C per hour, and material is at container
Middle thickness 50cm；
(3) frozen material is in 70 DEG C of water-baths, defrosting 40min；
(4) step (2)-(3) 2 times repeatedly, obtain thawing solution；
(5) thawing solution adds the water dilution of thawing solution quality 10 times, uses NaHCO3Adjust pH value to 8；
(6) the diluent colloid mill that step (5) obtains grinds 3-5min, obtains homogeneous liquid；
(7) homogeneous liquid adjusts pH value to 6-7, at 4 DEG C, centrifugal 20min under 5000rpm, collects supernatant,
Obtain nucleic acid extraction liquid；
(8) nucleic acid extraction liquid adds neutral protease, at 50 DEG C, react 4h, obtain enzymolysis solution, in system
In enzyme concentration be 2.5% (W/W), enzyme denaturing；
(9) adding sodium chloride in enzymolysis solution, sodium chloride mass content in enzymolysis solution is 8%, centrifugal, receives
Collection precipitation, adds the distilled water diluting of 2 times amount, and the diluent method of following (a)～(c) processes, it is thus achieved that
Different nucleic acid products:
A () diluent NF membrane vacuum filtration removes sodium chloride, be dried, obtain nucleic acid raw material, wherein said
Drying condition is: use drying under reduced pressure case, is dried 12h under 0.09Mpa, and material thickness is 1.0cm.Finally
The water content of the nucleic acid raw material obtained is less than 5%, and Nucleic acid quality content is more than 90wt%.
(b) diluent with NF membrane vacuum filtration remove sodium chloride, be equipped with in nucleic acid extraction liquid thickening agent,
Sweeting agents etc. can make natural acid nutritional solution.Appropriate sweeting agent it is equipped with former with dextrin etc. in nucleic acid extraction liquid
Material, drying, granulating process can make nutritive nucleic acid electuary.
C () diluent removes sodium chloride with NF membrane vacuum filtration after, local flavor flavoring agent etc. can be made.
Embodiment 2
From sorghum rice, Semen Maydis, Semen setariae, Fructus Hordei Vulgaris, Semen Tritici aestivi, extract nucleic acid, comprise the steps:
(1) taking sorghum rice (or Semen Maydis, Semen setariae, Fructus Hordei Vulgaris, Semen Tritici aestivi) and grind to form powder, granularity is 80-100
Mesh, adds the distilled water of its quality 15%；
(2) freezing 10 hours at-15 DEG C, keep material cooling rate 1-2 DEG C per hour, and material is holding
Thickness 50cm in device；
(3) frozen material is in 75 DEG C of water-baths, defrosting 40min；
(4) step (2)-(3) 2 times repeatedly, obtain thawing solution；
(5) defrosting adds the water dilution of thawing solution quality 8 times in night, adjusts pH value to 8 with NaHCO3；
(4) the diluent colloid mill that step (5) obtains grinds 3-5min；Obtain homogeneous liquid；
(5) homogeneous liquid adjust pH value to 6-7, at 4 DEG C, centrifugal 10-20min under 5000rpm, in collection
Clear liquid, obtains nucleic acid extraction liquid；
(6) nucleic acid extraction liquid adds neutral protease, at 50 DEG C, react 4h, obtain enzymolysis solution, in system
In enzyme concentration be 1.0% (W/W)；
(7) adding sodium chloride in enzymolysis solution, chlorination diluent uses following (a)～(c) sodium in enzymolysis solution
Mass content is 8%, centrifugal, collects precipitation, adds the distilled water diluting of 2 times amount, method process, it is thus achieved that
Different nucleic acid products:
A () diluent NF membrane vacuum filtration removes sodium chloride, be dried, obtain nucleic acid raw material, wherein said
Drying condition is: use drying under reduced pressure case, is dried 12h under 0.09Mpa, and material thickness is 1.0cm.Finally
The water content of the nucleic acid raw material obtained is less than 5%, and nucleic acid content is more than 85wt%.
(b) diluent with NF membrane vacuum filtration remove sodium chloride, be equipped with in nucleic acid extraction liquid thickening agent,
Sweeting agents etc. can make natural acid nutritional solution.Appropriate sweeting agent it is equipped with former with dextrin etc. in nucleic acid extraction liquid
Material, drying, granulating process can make nutritive nucleic acid electuary.
C () diluent removes sodium chloride with NF membrane vacuum filtration after, local flavor flavoring agent etc. can be made.
Claims (7)
1. the method extracting nucleic acid from wholefood, comprises the steps:
(1) wholefood is ground to form powder or pastel；
(2) distilled water of the 5-25% of powder or pastel quality, slow freezing are added；
(3) frozen material thaws in 65-80 DEG C of water-bath；
(4) thawing solution that step (3) obtains adds water dilution, tune pH value to 8-10；
(5) the diluent colloid mill that step (4) obtains grinds 3-5min, obtains homogeneous liquid；
(6) homogeneous liquid adjusts pH value to 6-7, is centrifuged, and collects supernatant, obtains nucleic acid extraction liquid；
(7) nucleic acid extraction liquid adds neutral protease, at 45-55 DEG C, react 4-6h, obtain enzymolysis solution；
(8) enzymolysis solution adds sodium chloride, centrifugal, collect precipitation, add distilled water diluting, use NF membrane
Vacuum filtration removes sodium chloride, is dried, obtains nucleic acid raw material.
The method of extraction nucleic acid the most according to claim 1, it is characterised in that in step (4),
Described thawing solution is 1:5-10 with the mass ratio of the water of addition.
The method of extraction nucleic acid the most according to claim 1, it is characterised in that in step (6)
Described centrifugal condition is: at 4 DEG C, with 10-20min centrifugal under 3000-5000rpm.
The method of extraction nucleic acid the most according to claim 1, it is characterised in that in step (8),
Adding sodium chloride in enzymolysis solution is 8-12% to making sodium chloride weight/mass percentage composition in enzymolysis solution.
The method of extraction nucleic acid the most according to claim 1, it is characterised in that in step (8),
Described precipitation and the mass ratio of the water of addition are 1:1-2.
The method of extraction nucleic acid the most according to claim 1, it is characterised in that described wholefood is
Semen Phaseoli, Semen phaseoli radiati, Semen Viciae fabae, Semen Pisi sativi, sorghum rice, Semen Maydis, Semen setariae, Fructus Hordei Vulgaris or Semen Tritici aestivi.
The method of extraction nucleic acid the most according to claim 1, it is characterised in that in step (8),
Nucleic acid solution is dried condition: use heated-air circulation oven, is dried 2-4h at 65-75 DEG C, and material thickness is
0.5-1.5cm。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610348190.5A CN105815663A (en) | 2016-05-24 | 2016-05-24 | Method for extracting nucleic acid raw material from natural foods |
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| CN201610348190.5A CN105815663A (en) | 2016-05-24 | 2016-05-24 | Method for extracting nucleic acid raw material from natural foods |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355284A (en) * | 2018-11-28 | 2019-02-19 | 罗锋利 | A kind of extracting method of yeast nucleic acid and its application in production selenium-rich nucleic acid |
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Application publication date: 20160803 |