CN101775417A - Method for extracting anthocyanin from blueberry pomace - Google Patents
Method for extracting anthocyanin from blueberry pomace Download PDFInfo
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- CN101775417A CN101775417A CN201010032422A CN201010032422A CN101775417A CN 101775417 A CN101775417 A CN 101775417A CN 201010032422 A CN201010032422 A CN 201010032422A CN 201010032422 A CN201010032422 A CN 201010032422A CN 101775417 A CN101775417 A CN 101775417A
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Abstract
The invention discloses a method for extracting anthocyanin from blueberry pomace. The method aims to provide a simple, efficient and energy-saving method for efficiently using blueberry resources. The adopted technical proposal is as follows: taking the blueberry pomace after producing juice or fruit wine as a raw material; and adopting the culture and extraction technology of integrated enzyme-cavitation suspension to extract the anthocyanin from the pomace. The method belongs to the high efficient utilization of biological resources, is simple and practicable, is suitable for the industry production and has important significance to the efficient utilization of the blueberry resources.
Description
Technical field
The present invention relates to a kind of from plant the method for extraction functionality composition, specifically be meant the processing method of from blueberry pomace, carrying anthocyanidin.
Background technology
Blueberry (blueberry) is perennial fallen leaves of Ericaceae Vaccinium or evergreen fruit trees, mostly is shrub.Be distributed widely in the Northern Hemisphere, mainly produce in Daxing'an Mountainrange and forest zone, the Xiaoxinanlin Mountains in China.Its fruit is a berry, is rich in multiple biologically active substances such as anthocyanidin, flavones.
Blueberry has the strong anti-oxidation activity, can promote the erythrolabe resynthesis, improve circulation, antiulcer agent, anti-inflammatory, enhance immunity power, cardiac function enhancing, anti-cardiovascular disease, delay senility, multiple physiologically active (Ling Guanting such as anticancer and anti-mutation, can supply developing food products additive (IX): blue certain kind of berries extract and antioxygenation thereof. grain and grease, 2003,6:45～48; Sun Zhijian, Zhang Yan, Chen Fang is to the thinking of blueberry industrialized development. foodstuffs industry science and technology, 2005,12:183～184).These health efficacies that possess just because of blueberry make that the demand of blueberry presents the trend that rises rapidly on the world market in recent years, and blueberry is the big fruit in the third place in the world after apple and citrus.
Current, the blueberry industry of countries in the world is flourish, and the blueberry product form is varied, and wherein fruit juice product is quite a lot of.But, it is reported that the anthocyanidin that has only 13-23% and the chlorogenic acid of 36-39% are present in the blueberry juice of pasteurize, about respectively 42% and 15% remains in the pomace; In addition, contain the anthocyanidin of 33.6mg during the 100g blueberry is just squeezed the juice, and contain 184mg in the 100g pomace.Therefore,, be necessary the extraction process of anthocyanidin in the blueberry pomace is studied, thereby obtain anthocyanidin extracting method in a kind of efficient, energy-conservation, safe blueberry pomace from the angle of rational exploitation and utilization vaccinium oxycoccus pigment resource.
Traditional water extraction or organic solvent extraction method exist extracts rate variance, and raw material availability is low, and problem such as environmental pollution is big; Though modern supercutical fluid method extraction yield height because of apparatus expensive, is not suitable for suitability for industrialized production.The enzyme assisted extraction is on the basis of extracting, utilize biological enzymatic reaction (as hemicellulase, cellulase, polygalacturonase, proteolytic enzyme etc.) to destroy combining of cell wall structure or target component and Mierocrystalline cellulose, protein etc., effective constituent is discharged, thereby improve extraction efficiency.And cavitation phenomenon is to be changed by the liquid internal local pressure, thus on liquid internal or liquid-solid interface, cause the gas cavity formation, develop and crumble and fall.When occurring near the solid surface when crumbling and fall, cavity moment crumbles and fall and produces very high instantaneous pressure, destroys solid surface.The cavitation extractive technique is to utilize cavitation bubble to produce intensive cavitation effect and mechanical vibration (Li Gensheng etc., 1997; Gao Qiusheng, 1999), cause the sample particle cell walls to break fast, quickened intracellular organic matter and discharged, spread and dissolving, thereby promoted to extract to medium.Though, the report of much handling blue berry with biological enzyme and then extracting anthocyanidin is arranged now, but all be to be used to be applicable to lab analysis, have that treatment capacity is little, needs add that aggressive solvent is regulated buffer system, need be kept certain temperature, the enzyme incubation time is long and cultivate and extract operation and shortcoming such as carry out respectively, therefore are unsuitable for suitability for industrialized production.
The present invention is intended to set up and a kind ofly has collection and cultivate, extract integrated, and mass-transfer efficiency height, the extracting method that energy consumption is low, cost is low realize that the high efficiency extraction of anthocyanidin in the blueberry pomace and the maximization of blueberry resource use.
Summary of the invention
The object of the present invention is to provide a kind of is raw material with the blueberry pomace, is power with the cavitation suspendible, adopts the cultivation extraction of integrated enzyme-cavitation suspendible and ethanol to strengthen extraction anthocyanidin.This method is simple, the target compound loss is few.The objective of the invention is to reach by following scheme:
Utilize negative pressure or just pressing in the system to feed nitrogen or air provides cavitation suspendible propulsion source, adopt the cultivation extraction of integrated enzyme-cavitation suspendible and ethanol to strengthen and extract anthocyanidin, extracting solution is static, filter and promptly get anthocyanidin extracting solution in the blueberry pomace.
The above-mentioned method of extracting anthocyanidin from blueberry pomace is characterized in that the pomace after described blueberry pomace is for production blueberry juice drink.
The above-mentioned method of from blueberry pomace, extracting anthocyanidin, the cultivation extraction and the ethanol reinforcement extraction anthocyanidin that it is characterized in that described enzyme-cavitation suspendible are realized by following operation: blueberry pomace adds cellulase or cellulase-polygalacturonase prozyme, place 40～60 ℃ of warm water, make water as cultivating the solvent that extracts, suspendible is cultivated and is extracted 10～30min, further use ethanol to strengthen then and extract 20～30min, resulting suspension is through precipitation, filtration, and filtered liquid obtains the anthocyanidin product after drying.
The above-mentioned method of from blueberry pomace, extracting anthocyanidin, it is characterized in that described enzyme is cellulase or cellulase-polygalacturonase prozyme, its consumption is 2.0～4.0mg/g blueberry pomace, and the proportion of composing of cellulase-polygalacturonase prozyme is 3: 1～7: 1.
The above-mentioned method of extracting anthocyanidin from blueberry pomace is characterized in that described warm water, and its temperature is 40～60 ℃, and its volumetric usage is 6～10 with the volume mass ratio of blueberry pomace.
The above-mentioned method of extracting anthocyanidin from blueberry pomace is characterized in that described ethanol strengthens that to extract solvent for use be edible ethanol, and its adding mode is that compensation type adds, and add-on is for being 40～50% with ethanol volume fraction after water in the system mixes.
Advantage of the present invention:
1. this method employing negative pressure or malleation are power, the mass-transfer efficiency height; Collection is cultivated, is extracted from one, and is simple, do not need to continue heating, and energy consumption is low; Can realize large-scale industrialization production.
2. product yield height.
Specific embodiments
Embodiment 1
Take by weighing blueberry pomace 10g, add cellulase 30mg, 50 ℃ of warm water of 60mL, feed nitrogen, and be that power carries out negative pressure-cavitation homogenous solid-liquid phase and cultivate to extract 20min with the 0.05MPa negative pressure, replenish then and add edible ethanol 40mL, strengthen and extract 20min, extracting solution is static, filtration obtains blueberry pomace anthocyanidin extracting solution, obtains cyanidin extract after concentrating.
Embodiment 2
Take by weighing blueberry pomace 10g, add 5: 1 cellulase of mass ratio-polygalacturonase prozyme 24mg, 50 ℃ of warm water of 60mL, feed nitrogen, and be that power carries out negative pressure-cavitation homogenous solid-liquid phase and cultivate to extract 20min with the 0.05MPa negative pressure, replenish then to add edible ethanol 40mL, strengthen and extract 20min, extracting solution is static, filtration obtains blueberry pomace anthocyanidin extracting solution, obtains cyanidin extract after concentrating.
Embodiment 3
Take by weighing blueberry pomace 10g, add 40 ℃ of warm water of 60mL, bubbling air, and malleation is that power carries out cavitation suspendible cultivation extraction 20min, replenish then and add edible ethanol 40mL, strengthen and extract 20min, extracting solution is static, filtration obtains blueberry pomace anthocyanidin extracting solution, obtains cyanidin extract after concentrating.
Claims (7)
1. method of from blueberry pomace, extracting anthocyanidin, be primarily characterized in that: be raw material with the blueberry pomace, with the cavitation suspendible is power, adopt the cultivation extraction and the ethanol of integrated enzyme-cavitation suspendible to strengthen extraction anthocyanidin, extracting solution is static, filtration promptly gets anthocyanidin extracting solution in the blueberry pomace.
2. according to the described method of from blueberry pomace, extracting anthocyanidin of claim 1, it is characterized in that the pomace after described blueberry pomace is for production blueberry juice or fruit wine.
3. according to the described method of from blueberry pomace, extracting anthocyanidin of claim 1, it is characterized in that described cavitation suspendible power utilizes negative pressure or just pressing to feeding nitrogen or air realization in the system.
4. according to the described method of from blueberry pomace, extracting anthocyanidin of claim 1, the cultivation extraction and the ethanol reinforcement extraction anthocyanidin that it is characterized in that described enzyme-cavitation suspendible are realized by following operation: blueberry pomace adds cellulase or cellulase-polygalacturonase prozyme, place 40～60 ℃ of warm water, make water as cultivating the solvent that extracts, suspendible is cultivated and is extracted 10～30min, further use ethanol to strengthen then and extract 20～30min, resulting suspension is through precipitation, filtration, and filtered liquid obtains the anthocyanidin product after drying.
5. according to claim 1 or the 4 described methods of from blueberry pomace, extracting anthocyanidin, it is characterized in that described enzyme is cellulase or cellulase-polygalacturonase prozyme, its consumption is 2.0～4.0mg/g blueberry pomace, and the proportion of composing of cellulase-polygalacturonase prozyme is 3: 1～7: 1.
6. according to claim 1 or the 4 described methods of extracting anthocyanidin from blueberry pomace, it is characterized in that described warm water, its temperature is 40～60 ℃, and its volumetric usage is 6～10mL/g with the volume mass ratio of blueberry pomace.
7. according to claim 1 or the 4 described methods of from blueberry pomace, extracting anthocyanidin, it is characterized in that it is edible ethanol that described ethanol is strengthened the extraction solvent for use, its adding mode is that compensation type adds, and the ethanol volume fraction is 40～50% to add-on in order to mix afterwards with water in the system.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010032422A CN101775417A (en) | 2010-01-06 | 2010-01-06 | Method for extracting anthocyanin from blueberry pomace |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321062A (en) * | 2011-06-08 | 2012-01-18 | 吉林市新科奇保健食品有限公司 | Separating, purifying and inspecting method of anthocyanin in blueberry wine dregs |
| CN102432579A (en) * | 2011-11-28 | 2012-05-02 | 湖州师范学院 | Process for extracting anthocyanin from blueberry pomace |
| CN104109403A (en) * | 2013-12-10 | 2014-10-22 | 大兴安岭神州北极蓝莓生物工程有限公司 | New wild blueberry anthocyanin extraction and purification method |
| CN106749151A (en) * | 2016-11-28 | 2017-05-31 | 沈阳农业大学 | A kind of rapid extracting method of blueberry pomace anthocyanidin |
| CN107011309A (en) * | 2017-04-21 | 2017-08-04 | 沈阳市皇冠蓝莓产业有限公司 | A kind of preparation method of Anthocyanin from Blueberry |
| CN107125675A (en) * | 2017-04-14 | 2017-09-05 | 安徽金鹰农业科技有限公司 | A kind of preparation method of blueberry residue flavouring-soy-sauce |
| US9969707B2 (en) | 2011-12-16 | 2018-05-15 | CENTRE DE RECHERCHE INDUSTRIELLE DU QUéBEC | Method for extracting anthocyanin derivatives from a plant source |
| CN108727327A (en) * | 2017-04-19 | 2018-11-02 | 南阳师范学院 | Black fruit fructus lycii anthocyanidin extraction separation and purification method |
| CN110496415A (en) * | 2019-07-31 | 2019-11-26 | 武汉大学 | A kind of injection type cavitation extracts method, application and its device used of anthocyanin |
| CN111771916A (en) * | 2020-07-09 | 2020-10-16 | 安徽农业大学 | Rice cadmium-reducing leaf surface resistance control agent rich in anthocyanin and application method thereof |
-
2010
- 2010-01-06 CN CN201010032422A patent/CN101775417A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321062A (en) * | 2011-06-08 | 2012-01-18 | 吉林市新科奇保健食品有限公司 | Separating, purifying and inspecting method of anthocyanin in blueberry wine dregs |
| CN102432579A (en) * | 2011-11-28 | 2012-05-02 | 湖州师范学院 | Process for extracting anthocyanin from blueberry pomace |
| US9969707B2 (en) | 2011-12-16 | 2018-05-15 | CENTRE DE RECHERCHE INDUSTRIELLE DU QUéBEC | Method for extracting anthocyanin derivatives from a plant source |
| CN104109403A (en) * | 2013-12-10 | 2014-10-22 | 大兴安岭神州北极蓝莓生物工程有限公司 | New wild blueberry anthocyanin extraction and purification method |
| CN104109403B (en) * | 2013-12-10 | 2016-04-20 | 大兴安岭神州北极蓝莓生物工程有限公司 | Wild blueberry anthocyanidin extracts, Novel purification method |
| CN106749151A (en) * | 2016-11-28 | 2017-05-31 | 沈阳农业大学 | A kind of rapid extracting method of blueberry pomace anthocyanidin |
| CN106749151B (en) * | 2016-11-28 | 2020-02-07 | 沈阳农业大学 | Method for rapidly extracting blueberry pomace anthocyanin |
| CN107125675A (en) * | 2017-04-14 | 2017-09-05 | 安徽金鹰农业科技有限公司 | A kind of preparation method of blueberry residue flavouring-soy-sauce |
| CN108727327A (en) * | 2017-04-19 | 2018-11-02 | 南阳师范学院 | Black fruit fructus lycii anthocyanidin extraction separation and purification method |
| CN107011309A (en) * | 2017-04-21 | 2017-08-04 | 沈阳市皇冠蓝莓产业有限公司 | A kind of preparation method of Anthocyanin from Blueberry |
| CN110496415A (en) * | 2019-07-31 | 2019-11-26 | 武汉大学 | A kind of injection type cavitation extracts method, application and its device used of anthocyanin |
| CN110496415B (en) * | 2019-07-31 | 2021-01-01 | 武汉大学 | Method for extracting anthocyanin by injection type cavitation, application and device used by method |
| CN111771916A (en) * | 2020-07-09 | 2020-10-16 | 安徽农业大学 | Rice cadmium-reducing leaf surface resistance control agent rich in anthocyanin and application method thereof |
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Application publication date: 20100714 |