CN106749151B - Method for rapidly extracting blueberry pomace anthocyanin - Google Patents
Method for rapidly extracting blueberry pomace anthocyanin Download PDFInfo
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- CN106749151B CN106749151B CN201611059315.9A CN201611059315A CN106749151B CN 106749151 B CN106749151 B CN 106749151B CN 201611059315 A CN201611059315 A CN 201611059315A CN 106749151 B CN106749151 B CN 106749151B
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- blueberry pomace
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention relates to a method for quickly extracting blueberry pomace anthocyanin, which is characterized in that frozen blueberry pomace is taken as a raw material, the frozen blueberry pomace is beaten into pulp by a beater, after being treated by an ultrahigh pressure device at a certain temperature, pectinase, hemicellulase and cellulase are added into the pomace for enzymolysis, the mixture is mixed with an ethanol-acetic acid-water solvent after the enzymolysis, a high-pressure homogenizer is used for treating and extracting the anthocyanin, the pomace and anthocyanin extracting solution are separated by a centrifugal machine, the ethanol and the acetic acid in the extracting solution are removed by a decompression concentration mode, and then the mixture is put into a vacuum freeze-drying machine for freeze-drying to obtain the blueberry pomace anthocyanin. The technical scheme provided by the invention has the advantages of simple process operation, short period, low cost and low pollution, is suitable for large-batch continuous production, realizes comprehensive utilization of blueberries and has good application prospect.
Description
Technical Field
The invention relates to a method for quickly extracting blueberry pomace anthocyanin.
Background
The blueberry is used as a small berry with good flavor quality and high nutritional value, has high nutritional health care value and economic value, is popular in countries such as Europe and America, is gradually developed into a third generation emerging fruit, and has high economic benefit. Anthocyanins are natural and effective antioxidants, and have effects in protecting vision, and treating diabetes and cardiovascular diseases. The blueberry has high anthocyanin content, most of the anthocyanin exists in pomace, and the blueberry has good physiological function, so the blueberry is a good source of the anthocyanin. The traditional extraction method uses solvents such as water, alcohol and the like, and anthocyanin is extracted by processes such as heating, ultrasonic wave assistance and the like. Since the heat treatment has a great influence on the stability of anthocyanin, the traditional extraction method causes great loss of anthocyanin during the extraction process. In addition, the traditional process needs relatively long extraction time and large energy consumption, and is not beneficial to continuous production.
Disclosure of Invention
In order to overcome the technical problems of long period and high anthocyanin loss in the extraction process of blueberry pomace anthocyanin, the invention aims to provide a method for quickly extracting blueberry pomace anthocyanin.
The method for rapidly extracting the blueberry pomace anthocyanin provided by the invention comprises the following steps:
the operation process comprises the following steps:
frozen blueberry pomace → unfreezing → pulping → ultrahigh pressure treatment → ethanol-acetic acid-aqueous solution extraction → high pressure homogenization treatment → centrifugal separation and filtration → anthocyanin extracting solution → reduced pressure concentration → vacuum freeze drying → product.
The specific operation process is as follows:
(1) freezing blueberry pomace, unfreezing, beating into homogenate by a homogenizer, and performing ultrahigh pressure treatment by using an ultrahigh pressure device;
(2) adding the following components in percentage by weight into the pomace treated in the step (1): 5 per mill of pectinase, 3 per mill of hemicellulase and 3 per mill of cellulase, and performing enzymolysis for 2 hours at 50 ℃;
(3) adding an ethanol-acetic acid-water mixed solution after enzymolysis, wherein the volume ratio of the ethanol to the acetic acid to the water is 40:10:50, the material ratio is 1 g:5 mL, and treating the mixture by using a high-pressure homogenizer after uniformly mixing;
(4) separating and filtering the fruit residues and the anthocyanin extracting solution of the turbid solution obtained in the step (3) by a centrifugal machine, and removing ethanol and acetic acid from the anthocyanin extracting solution by a reduced-pressure rotary evaporation device;
(5) and (5) freeze-drying the anthocyanin aqueous solution obtained in the step (4) in a vacuum freeze dryer to obtain blueberry anthocyanin powder.
The beneficial effects obtained by the invention mainly comprise:
1. by adopting an ultrahigh pressure treatment mode, the tissue structure of the blueberry pomace is greatly damaged under the condition of non-thermal processing, and meanwhile, an acidic ethanol solution is mixed with the blueberry pomace, so that extremely favorable conditions are created for subsequent rapid extraction of anthocyanin.
2. By using a high-pressure homogeneous extraction mode and matching with an organic solvent extracting solution, anthocyanin in blueberry pomace can be fully dissolved in the organic solvent in a short time without heating, the extraction time is short, the production period is greatly shortened, the energy consumption is reduced, the extraction temperature is low, and the obtained blueberry anthocyanin powder has high extraction amount, high purity and less impurities.
Detailed Description
Example 1
Taking 1kg of frozen blueberry pomace, unfreezing at normal temperature, pulping into homogenate by using a homogenizer, and processing for 5min at 40 ℃ in an ultrahigh pressure device under 350 MPa. 5g of pectinase, 3g of hemicellulase and 3g of cellulase are added into the fruit residues after the ultrahigh pressure treatment, and the mixture is subjected to enzymolysis for 2 hours at the temperature of 50 ℃. Adding 5L ethanol-acetic acid-water (40: 10:50, v/v/v) solution into the residue after enzymolysis, and treating for 7min at 2000 r/min with high pressure homogenizer. And separating the pomace and the anthocyanin extracting solution from the turbid solution obtained after high-pressure homogenization by using a centrifugal machine, and removing ethanol and acetic acid from the anthocyanin extracting solution by using a reduced-pressure rotary evaporation device at the temperature of 45 ℃ to obtain the blueberry pomace anthocyanin aqueous solution. And (3) placing the anthocyanin aqueous solution into a vacuum freeze dryer for freeze drying to obtain 4.8g of blueberry pomace anthocyanin powder.
Example 2
Taking 10kg of frozen blueberry pomace, unfreezing at normal temperature, pulping into homogenate by using a homogenizer, and processing for 7min at 40 ℃ in an ultrahigh pressure device under 350 MPa. Adding 50g of pectinase, 30g of hemicellulase and 30g of cellulase into the fruit residues subjected to ultrahigh pressure treatment, and carrying out enzymolysis for 2h at 50 ℃. Adding 50L ethanol-acetic acid-water (40: 10:50, v/v/v) solution into the residue after enzymolysis, and treating for 7min at 2000 r/min by using a high-pressure homogenizer. And separating the pomace and the anthocyanin extracting solution from the turbid solution obtained after high-pressure homogenization by using a centrifugal machine, and removing ethanol and acetic acid from the anthocyanin extracting solution by using a reduced-pressure rotary evaporation device at the temperature of 45 ℃ to obtain the blueberry pomace anthocyanin aqueous solution. And (3) placing the anthocyanin aqueous solution into a vacuum freeze dryer for freeze drying to obtain 49.5g of blueberry pomace anthocyanin powder.
Example 3
Taking 20kg of frozen blueberry pomace, unfreezing at normal temperature, pulping into homogenate by using a homogenizer, and treating for 7min at 40 ℃ in an ultrahigh pressure device under 350 MPa. Adding 100g of pectinase, 60g of hemicellulase and 60g of cellulase into the fruit residues subjected to ultrahigh pressure treatment, and carrying out enzymolysis for 2h at 50 ℃. Adding 100L ethanol-acetic acid-water (40: 10:50, v/v/v) solution into the residue after enzymolysis, and treating for 7min at 2000 r/min with high pressure homogenizer. And separating the pomace and the anthocyanin extracting solution from the turbid solution obtained after high-pressure homogenization by using a centrifugal machine, and removing ethanol and acetic acid from the anthocyanin extracting solution by using a reduced-pressure rotary evaporation device at the temperature of 45 ℃ to obtain the blueberry pomace anthocyanin aqueous solution. And (3) placing the anthocyanin aqueous solution into a vacuum freeze dryer for freeze drying to obtain 99.3g of blueberry pomace anthocyanin powder.
Claims (3)
1. A method for rapidly extracting blueberry pomace anthocyanin is characterized by comprising the following steps:
(1) freezing blueberry pomace, beating into homogenate with the refiner after unfreezing to use super high-pressure equipment to carry out the superpressure treatment, the superpressure treatment is: the pressure is 350MPa, the temperature is 40 ℃, and the treatment time is 5 min;
(2) adding the following components in percentage by weight into the pomace treated in the step (1): 5 per mill of pectinase, 3 per mill of hemicellulase and 3 per mill of cellulase, and performing enzymolysis for 2 hours at 50 ℃;
(3) adding an ethanol-acetic acid-water mixed solution after enzymolysis, wherein the volume ratio of the ethanol to the acetic acid to the water is 40:10:50, the material ratio is 1 g:5 mL, uniformly mixing, and extracting by using a high-pressure homogenizer at the revolution of 2000 r/min for 7-10 min;
(4) separating and filtering the fruit residues and the anthocyanin extracting solution of the turbid solution obtained in the step (3) by a centrifugal machine, and removing ethanol and acetic acid from the anthocyanin extracting solution by a reduced-pressure rotary evaporation device;
(5) and (5) freeze-drying the anthocyanin aqueous solution obtained in the step (4) in a vacuum freeze dryer to obtain blueberry anthocyanin powder.
2. The method for rapidly extracting anthocyanin from blueberry pomace as claimed in claim 1, wherein the mesh number of the filter screen of the centrifuge in the step (4) is 200 meshes, and the reduced pressure concentration temperature of the reduced pressure rotary evaporation device is 45 ℃.
3. The method for rapidly extracting the anthocyanin from the blueberry pomace as defined in claim 1, wherein the temperature of the feed liquid in the steps (1) to (5) is controlled below 50 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611059315.9A CN106749151B (en) | 2016-11-28 | 2016-11-28 | Method for rapidly extracting blueberry pomace anthocyanin |
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| CN201611059315.9A CN106749151B (en) | 2016-11-28 | 2016-11-28 | Method for rapidly extracting blueberry pomace anthocyanin |
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| CN106749151A CN106749151A (en) | 2017-05-31 |
| CN106749151B true CN106749151B (en) | 2020-02-07 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108094614B (en) * | 2017-12-08 | 2021-12-28 | 宜春蓝玉有机农业开发有限公司 | Preparation method of blueberry anthocyanin tea |
| CN108719395A (en) * | 2018-05-21 | 2018-11-02 | 遵义盛林农业发展有限公司 | A kind of production method for blueberry biscuit |
| CN109511980A (en) * | 2018-12-17 | 2019-03-26 | 贵州理工学院 | A kind of preparation method of blueberry pomace extract dry powder |
| CN110256393B (en) * | 2019-06-17 | 2022-12-27 | 上海交通大学 | Method for extracting akebia trifoliata anthocyanin by using radio frequency-assisted biological complex enzyme |
| CN110698450A (en) * | 2019-11-04 | 2020-01-17 | 哈尔滨工业大学 | Ultrasonic-homogenizing-ultrasonic circulating extraction method for blueberry anthocyanin |
| CN112515067A (en) * | 2020-12-14 | 2021-03-19 | 合肥工业大学 | Processing method of pomegranate juice with low loss of plant polyphenol and anthocyanin |
| CN113956679A (en) * | 2021-11-19 | 2022-01-21 | 长江师范学院 | Method for rapidly extracting carmine radish anthocyanin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101775417A (en) * | 2010-01-06 | 2010-07-14 | 高畅 | Method for extracting anthocyanin from blueberry pomace |
| CN104860916A (en) * | 2015-04-07 | 2015-08-26 | 南京晓庄学院 | Method for extracting proanthocyanidins from apple peel |
| CN105272956A (en) * | 2015-11-13 | 2016-01-27 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrial method for preparing roselle anthocyanin from roselle |
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2016
- 2016-11-28 CN CN201611059315.9A patent/CN106749151B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101775417A (en) * | 2010-01-06 | 2010-07-14 | 高畅 | Method for extracting anthocyanin from blueberry pomace |
| CN104860916A (en) * | 2015-04-07 | 2015-08-26 | 南京晓庄学院 | Method for extracting proanthocyanidins from apple peel |
| CN105272956A (en) * | 2015-11-13 | 2016-01-27 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrial method for preparing roselle anthocyanin from roselle |
Non-Patent Citations (1)
| Title |
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| 响应曲面法优化原花青素的高压微波提取工艺研究;李超等;《食品科学》;20091231;第30卷(第18期);39-43 * |
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