CN106749151A - A kind of rapid extracting method of blueberry pomace anthocyanidin - Google Patents
A kind of rapid extracting method of blueberry pomace anthocyanidin Download PDFInfo
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- CN106749151A CN106749151A CN201611059315.9A CN201611059315A CN106749151A CN 106749151 A CN106749151 A CN 106749151A CN 201611059315 A CN201611059315 A CN 201611059315A CN 106749151 A CN106749151 A CN 106749151A
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- anthocyanidin
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- blueberry
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of rapid extracting method of blueberry pomace anthocyanidin, the present invention is to freeze blueberry pomace as raw material, pulpous state is broken into by beater, after being processed using ultra-high pressure apparatus at a certain temperature, to adding pectase in pomace, hemicellulase, cellulase degradation, mix with alcohol, acetic acid aqueous solvent after enzymolysis, and process extraction anthocyanidin using high pressure homogenizer, pomace is separated with anthocyanidin extract solution by centrifuge, and gone out in extract solution after ethanol and acetic acid using mode concentrated under reduced pressure, it is put into lyophilized prepared blueberry pomace anthocyanidin in vacuum freeze drier.The technical scheme technological operation for providing of the invention is simple, cycle is short is compared with cost is relatively low, less pollution, is adapted to high-volume continuous prodution, realizes the comprehensive utilization of blueberry, has a good application prospect.
Description
Technical field
The present invention relates to a kind of rapid extracting method of blueberry pomace anthocyanidin.
Background technology
The small berries that blueberry is excellent as a kind of flavor quality, be of high nutritive value, have better nutritivity health value and economy
Value, is well received in countries such as America and Europes, has gradually developed into the emerging fruit of the third generation, with economic effect very high
Benefit.Anthocyanidin is pure natural effective antioxidant, and has treatment with protection eyesight, to diabetes and angiocardiopathy
Effect.The content of anthocyanidin is higher in blueberry, and is mostly present in pomace, and it has good physiological function,
Therefore it is the good source of anthocyanidin.Traditional extraction process uses water, alcohol equal solvent, by techniques such as heating, ultrasonic assistants
Extract anthocyanidin.There is extreme influence to anthocyanidin stability due to being heat-treated, therefore traditional extraction process is in extraction process
Greatly loss can be caused to anthocyanidin.Additionally, traditional handicraft needs extraction time relatively long, energy consumption is larger, is unfavorable for connecting
Continuous production.
The content of the invention
In order in overcoming blueberry pomace anthocyanidin extraction process, the cycle is long, anthocyanidin loses big technical problem, the present invention
Purpose be to provide a kind of rapid extracting method of blueberry pomace anthocyanidin, using carrying for ultra high pressure treatment and high-pressure homogeneous mode
Anthocyanidin is taken, blueberry anthocyanin is extracted by the way of non-thermal technology, shorten extraction time, realize the serialization of blueberry anthocyanin
Production, and it is with low cost, pollution-free, the blueberry anthocyanin impurity for being produced is few, high purity.
The rapid extracting method of the blueberry pomace anthocyanidin that the present invention is provided is as follows：
Operational sequence：
Freezing blueberry pomace → defrosting → mashing → ultra high pressure treatment → Ethanol-Acetic Acid-aqueous solution extraction → high-pressure homogeneous treatment
→ centrifugation filtering → anthocyanidin extract solution → concentrated under reduced pressure → vacuum freeze drying → product.
Specific operation process is：
（1）Freezing blueberry pomace, breaks into homogenate, and carry out ultra high pressure treatment using ultra-high pressure apparatus after defrosting with refiner;
（2）To step（1）The following composition of part by weight is added after treatment in gained pomace：5 ‰ pectases, 3 ‰ hemicelluloses
Enzyme, 3 ‰ cellulases, in digesting 2h at 50 DEG C；
（3）The mixed solution of Ethanol-Acetic Acid-water is added after enzymolysis, the volume ratio of Ethanol-Acetic Acid-water is 40:10:50, its material
Than being 1 g:5 mL, are processed after being well mixed using high pressure homogenizer；
（4）By step（3）Resulting turbid solution by centrifuge separating and filtering pomace and anthocyanidin extract solution, and by anthocyanidin
Extract and ethanol and acetic acid are removed by the rotary evaporating device that depressurizes；
（5）By step（4）The gained anthocyanidin aqueous solution is freezed using in vacuum freeze drier, and blueberry anthocyanin powder is obtained.
The beneficial effect that the present invention is obtained mainly includes：
1. by the way of ultra high pressure treatment, under conditions of non-thermal technology, the institutional framework of blueberry pomace is greatly destroyed,
Mixed with blueberry pomace using acid ethanol solution simultaneously, be the rapid extraction of follow-up anthocyanidin, created extremely
Favourable condition.
2. high-pressure homogeneous extracting mode is used, coordinates Solvent Extract methods liquid, can be short under conditions of without heating
When it is interior anthocyanidin in blueberry pomace is substantially soluble in organic solvent, extraction time is short, greatly shortens the production cycle, reduces
Energy consumption, Extracting temperature is low, and resulting blueberry anthocyanin powder extracted amount is high, high purity, impurity are few.
Specific embodiment
Embodiment 1
Take cold storage blueberry pomace 1kg, under normal temperature thaw after break into homogenate using refiner, be placed in ultra-high pressure apparatus in
350MPa, 5min is processed at 40 DEG C.To addition 5g pectases, 3g hemicellulases, 3g fibers in the pomace after ultra high pressure treatment
Plain enzyme, and in digesting 2h at 50 DEG C.Pomace after enzymolysis is added into 5L Ethanol-Acetic Acids-water（40:10:50, v/v/v）Solution, makes
With high pressure homogenizer in processing 7min under 2000 turns/min.Turbid solution obtained by after will be high-pressure homogeneous separates fruit by centrifuge
Slag and anthocyanidin extract solution, and by anthocyanidin extract solution by the rotary evaporating device that depressurizes, in removing ethanol and acetic acid at 45 DEG C,
Obtain the blueberry pomace anthocyanidin aqueous solution.The anthocyanidin aqueous solution is placed in vacuum freeze drier and is freezed, blueberry pomace is obtained
Anthocyanidin powder 4.8g.
Embodiment 2
Take cold storage blueberry pomace 10kg, under normal temperature thaw after break into homogenate using refiner, be placed in ultra-high pressure apparatus in
350MPa, 7min is processed at 40 DEG C.To being added in the pomace after ultra high pressure treatment, 50g pectases, 30g hemicellulases, 30g are fine
The plain enzyme of dimension, and in digesting 2h at 50 DEG C.Pomace after enzymolysis is added into 50L Ethanol-Acetic Acids-water（40:10:50, v/v/v）It is molten
Liquid, using high pressure homogenizer in processing 7min under 2000 turns/min.Turbid solution obtained by after will be high-pressure homogeneous passes through centrifuge
Pomace and anthocyanidin extract solution are separated, and by anthocyanidin extract solution by the rotary evaporating device that depressurizes, in removing ethanol at 45 DEG C
And acetic acid, obtain the blueberry pomace anthocyanidin aqueous solution.The anthocyanidin aqueous solution is placed in vacuum freeze drier and is freezed, indigo plant is obtained
Certain kind of berries pomace anthocyanidin powder 49.5g.
Embodiment 3
Take cold storage blueberry pomace 20kg, under normal temperature thaw after break into homogenate using refiner, be placed in ultra-high pressure apparatus in
350MPa, 7min is processed at 40 DEG C.To addition 100g pectases, 60g hemicellulases, 60g in the pomace after ultra high pressure treatment
Cellulase, and in digesting 2h at 50 DEG C.Pomace after enzymolysis is added into 100L Ethanol-Acetic Acids-water（40:10:50, v/v/v）
Solution, using high pressure homogenizer in processing 7min under 2000 turns/min.Turbid solution obtained by after will be high-pressure homogeneous is by centrifugation
Machine separates pomace and anthocyanidin extract solution, and by anthocyanidin extract solution by the rotary evaporating device that depressurizes, in removing second at 45 DEG C
Alcohol and acetic acid, obtain the blueberry pomace anthocyanidin aqueous solution.The anthocyanidin aqueous solution is placed in vacuum freeze drier and is freezed, be obtained
Blueberry pomace anthocyanidin powder 99.3g.
Claims (5)
1. a kind of rapid extracting method of blueberry pomace anthocyanidin, it is characterised in that specifically include following steps：
（1）Freezing blueberry pomace, breaks into homogenate, and carry out ultra high pressure treatment using ultra-high pressure apparatus after defrosting with refiner;
（2）To step（1）The following composition of part by weight is added after treatment in gained pomace：5 ‰ pectases, 3 ‰ hemicelluloses
Enzyme, 3 ‰ cellulases, in digesting 2h at 50 DEG C；
（3）The mixed solution of Ethanol-Acetic Acid-water is added after enzymolysis, the volume ratio of Ethanol-Acetic Acid-water is 40:10:50, its material
Than being 1 g:5 mL, are processed after being well mixed using high pressure homogenizer；
（4）By step（3）Resulting turbid solution by centrifuge separating and filtering pomace and anthocyanidin extract solution, and by anthocyanidin
Extract and ethanol and acetic acid are removed by the rotary evaporating device that depressurizes；
（5）By step（4）The gained anthocyanidin aqueous solution is freezed using in vacuum freeze drier, and blueberry anthocyanin powder is obtained.
2. a kind of rapid extracting method of blueberry pomace anthocyanidin according to claim 1, it is characterised in that the step
（1）Middle ultra high pressure is 350MPa, and temperature is 40 DEG C, and process time is 5min.
3. a kind of rapid extracting method of blueberry pomace anthocyanidin according to claim 1, it is characterised in that the step
（3）Middle to be extracted using high-pressure homogeneous, revolution is 2000 turns/min, and extraction time is 7-10min.
4. a kind of rapid extracting method of blueberry pomace anthocyanidin according to claim 1, it is characterised in that the step
（4）The filter screen mesh number of middle centrifuge is 200 mesh, and the temperature concentrated under reduced pressure of the rotary evaporating device that depressurizes is 45 DEG C.
5. a kind of rapid extracting method of blueberry pomace anthocyanidin according to claim 1, it is characterised in that the step
（1）-（5）Middle feed temperature control is below 50 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611059315.9A CN106749151B (en) | 2016-11-28 | 2016-11-28 | Method for rapidly extracting blueberry pomace anthocyanin |
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| CN201611059315.9A CN106749151B (en) | 2016-11-28 | 2016-11-28 | Method for rapidly extracting blueberry pomace anthocyanin |
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| CN106749151A true CN106749151A (en) | 2017-05-31 |
| CN106749151B CN106749151B (en) | 2020-02-07 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094614A (en) * | 2017-12-08 | 2018-06-01 | 宜春蓝玉有机农业开发有限公司 | A kind of preparation method of blueberry anthocyanin tea |
| CN108719395A (en) * | 2018-05-21 | 2018-11-02 | 遵义盛林农业发展有限公司 | A kind of production method for blueberry biscuit |
| CN109511980A (en) * | 2018-12-17 | 2019-03-26 | 贵州理工学院 | A kind of preparation method of blueberry pomace extract dry powder |
| CN110256393A (en) * | 2019-06-17 | 2019-09-20 | 上海交通大学 | A method of threeleaf akebia anthocyanidin is extracted using RF assistance biological complex enzyme |
| CN110698450A (en) * | 2019-11-04 | 2020-01-17 | 哈尔滨工业大学 | Ultrasonic-homogenizing-ultrasonic circulating extraction method for blueberry anthocyanin |
| CN112515067A (en) * | 2020-12-14 | 2021-03-19 | 合肥工业大学 | Processing method of pomegranate juice with low loss of plant polyphenol and anthocyanin |
| CN113956679A (en) * | 2021-11-19 | 2022-01-21 | 长江师范学院 | Method for rapidly extracting carmine radish anthocyanin |
Citations (3)
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| CN101775417A (en) * | 2010-01-06 | 2010-07-14 | 高畅 | Method for extracting anthocyanin from blueberry pomace |
| CN104860916A (en) * | 2015-04-07 | 2015-08-26 | 南京晓庄学院 | Method for extracting proanthocyanidins from apple peel |
| CN105272956A (en) * | 2015-11-13 | 2016-01-27 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrial method for preparing roselle anthocyanin from roselle |
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2016
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Patent Citations (3)
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| CN101775417A (en) * | 2010-01-06 | 2010-07-14 | 高畅 | Method for extracting anthocyanin from blueberry pomace |
| CN104860916A (en) * | 2015-04-07 | 2015-08-26 | 南京晓庄学院 | Method for extracting proanthocyanidins from apple peel |
| CN105272956A (en) * | 2015-11-13 | 2016-01-27 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrial method for preparing roselle anthocyanin from roselle |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094614A (en) * | 2017-12-08 | 2018-06-01 | 宜春蓝玉有机农业开发有限公司 | A kind of preparation method of blueberry anthocyanin tea |
| CN108094614B (en) * | 2017-12-08 | 2021-12-28 | 宜春蓝玉有机农业开发有限公司 | Preparation method of blueberry anthocyanin tea |
| CN108719395A (en) * | 2018-05-21 | 2018-11-02 | 遵义盛林农业发展有限公司 | A kind of production method for blueberry biscuit |
| CN109511980A (en) * | 2018-12-17 | 2019-03-26 | 贵州理工学院 | A kind of preparation method of blueberry pomace extract dry powder |
| CN110256393A (en) * | 2019-06-17 | 2019-09-20 | 上海交通大学 | A method of threeleaf akebia anthocyanidin is extracted using RF assistance biological complex enzyme |
| CN110256393B (en) * | 2019-06-17 | 2022-12-27 | 上海交通大学 | Method for extracting akebia trifoliata anthocyanin by using radio frequency-assisted biological complex enzyme |
| CN110698450A (en) * | 2019-11-04 | 2020-01-17 | 哈尔滨工业大学 | Ultrasonic-homogenizing-ultrasonic circulating extraction method for blueberry anthocyanin |
| CN112515067A (en) * | 2020-12-14 | 2021-03-19 | 合肥工业大学 | Processing method of pomegranate juice with low loss of plant polyphenol and anthocyanin |
| CN113956679A (en) * | 2021-11-19 | 2022-01-21 | 长江师范学院 | Method for rapidly extracting carmine radish anthocyanin |
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| Publication number | Publication date |
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| CN106749151B (en) | 2020-02-07 |
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