CN101948897A - Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn - Google Patents
Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn Download PDFInfo
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Abstract
The invention belongs to the deep processing technology of shinyleaf yellowhorn, in particular to a method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn. The method comprises the following steps of: taking the kernels of shinyleaf yellowhorn, crushing the shinyleaf yellowhorn kernels, and adding water to swell; continuously treating by using microwaves, hydrolyzing by using alkali protease, separating three phases by using neutral protease, flavourzyme, cellulase and hemicellulase, concentrating through ultrafiltration by using an ultrafiltration membrane, drying the concentrated solution, and vacuum-drying and crushing the hydrolyzing residue to obtain high-grade animal feed rich in protein, fat and cellulose. In a word, the invention overcomes various defects caused by the traditional vegetable oil preparation process and adopts a brand new process for furthest extracting pollution-free shinyleaf yellowhorn oil and simultaneously acquiring shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide. The whole processing process reflects continuity, rapidness and mechanization, and production efficiency is greatly improved.
Description
Technical field
The invention belongs to the Wood of Shinyleaf Yellowhorn deep process technology, be specifically related to a kind of method of from Wood of Shinyleaf Yellowhorn, extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide.
Background technology
Wood of Shinyleaf Yellowhorn is a Sapindaceae Wood of Shinyleaf Yellowhorn platymiscium, has another name called pawpaw, civil official and really is machaka or arbor, originates in northern China, grows in the barren hill cheuch and the hilly country at 52 ~ 2260 meters of height above sea level.Its seed oil length 30.4%, benevolence oil length 66.39% is planted in the peeling back, contains protein 25.75%.Unsaturated fatty acid content accounts for 36.9% oleic acid up to 94% linolic acid to account for 57.16% is the table oil abundanter more than sweet oil, tea oil nutrition in its oil., its protein content is also very abundant, it is the higher vegetable-protein kind of a kind of nutritive value, also contain more active polysaccharide in the Wood of Shinyleaf Yellowhorn, molecular-weight average also is a kind of and the functional product of tool exploitation at 10-15 about ten thousand.By force cold-resistant drought-enduring barren-resistant can extensively plantation of Wood of Shinyleaf Yellowhorn adaptability in addition in the hilly country, deserted mountain..Exploitation Wood of Shinyleaf Yellowhorn resource not only can planting trees on barren hills be checked winds and fixed drifting sand but also can be created economic worth.The main method of producing vegetables oil at present is traditional milling process and extraction.Milling process technology is simple, support equipment is few, the flavour that can keep product, but this method residual oil content height (7～10%), labour intensity is big, power consumption is big, cost is high, and the cake protein qualitative change degree height after the squeezing, can only be used as fertilizer, protein utilization rate is low, causes the proteic waste of a large amount of quality plants.Lixiviation process production efficiency height and residual oil content are low, but this method equipment is many, the investment big; The crude oil complicated component needs refining treatment, and the oil product flavor loss is big, and the oil colours pool of leaching is darker, also needs further refining; With an organic solvent increase the loaded down with trivial details property of technology, reduced the security of producing, caused the pollution of environment; Micro-organic solvent residual in the processed oil is unfavorable to human health.Protein polypeptide is the protein fragments that two or more amino acid connect into by peptide bond, and it is the ultimate constituent of body tissue cell.Since the importance of biological bioactive peptide in food and healthcare products is familiar with, adopted enzyme process extracts biologically active peptides from plant resources research also more and more widely.The Wood of Shinyleaf Yellowhorn protein peptide has the important physical function, and it can remove interior free yl, reaches effect anti-oxidant, that prolong cell survival.Its property digested and assimilated is good simultaneously, food safety is high, so can be used as nutrition-fortifying agent is applied to be regarded as " emerging nutritive health-care source " and " functional factor that has development potentiality " in baby and children's formula food, diet food, sportsmen's food and the dietetic food.The Wood of Shinyleaf Yellowhorn polysaccharide has multiple function, can blood fat reducing, controlling blood sugar, strengthening immunity, and anti-ly treat constipation, improve intestinal microenvironment etc., be widely used in medicine and field of health care food.The angle development that the utilization of Wood of Shinyleaf Yellowhorn component should be extracted, fully utilize towards total composition.Thus, pressing for a kind of new coproduction reparation technology of exploitation comes efficient, industrialization to produce high-quality, the shinyleaf yellowhorn oil of nutritive health-care and the method for Wood of Shinyleaf Yellowhorn protolysate peptide.
Application number is 200910019388.9 to disclose a kind of method of extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, method is to operate as follows: be 1: 4～8 to pour in the colloidal mill and pulverize Wood of Shinyleaf Yellowhorn Renhe water by solid-liquid ratio (g/mL) 1), pulverize Wood of Shinyleaf Yellowhorn benevolence to the 70-160 order, collect slurries; 2) pH regulator that will grind good slurries is 3.8-5.8, adds the cellulase of Wood of Shinyleaf Yellowhorn benevolence quality 0.5-2.5%, in 50-60 ℃ of water-bath enzymolysis 3-8 hour; 3) enzymolysis solution is centrifugal, 4000-6000 rev/min, centrifugal 20-30 minute; Behind centrifugal the finishing, draw upper strata edible vegetable oil; 4) pH with lower floor's enzymolysis solution is adjusted to 6.8-7.3, adds the Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence quality 1.8～2.5%, and reaction is 2.5-3.5 hour in 50-60 ℃ of water-bath; 5) with enzymolysis solution at 4500-6000 rev/min of centrifugal 30-35 minute, the yellow edible vegetable oil in sucking-off upper strata can merge with the edible vegetable oil of the sucking-off first time, also can use separately.Aforesaid method has mentioned that the use enzymolysis process extracts, but the enzyme that is to use is single, and the extracting substance kind that obtains is few, and of poor quality.
Summary of the invention
The purpose of this invention is to provide a kind of method of from Wood of Shinyleaf Yellowhorn, extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide.
The present invention is realized that by following scheme a kind of method of extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn may further comprise the steps:
1) get the benevolence of Wood of Shinyleaf Yellowhorn, Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds Wood of Shinyleaf Yellowhorn benevolence weight 20-30% simultaneously stirs, and places and makes its swelling in 1-2 hour;
2) the Wood of Shinyleaf Yellowhorn benevolence swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and handle, the frequency of microwave is 2400-2500MHz, and power is 60KW, 100 ℃-121 ℃ of temperature, transfer rate is 2.0-4.0m/min, and the time is 1.5-3.0 min;
3) the Wood of Shinyleaf Yellowhorn benevolence of microwave treatment is added 5-6 times of water of its weight, regulate pH value, add the Sumizyme MP of the 1-2% of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value at 8.5-9.5 under the 45-60 ℃ of condition, hydrolysis 2-3 hour to 8.5-9.5;
4) then at pH value 6.5-7.0, under the 45-60 ℃ of condition, each adds neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 2-4 hour of the 0.3-0.5% of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 80-100 ℃, stirs 10-20min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains oil phase, water and unhydrolysed residue solid phase respectively.Oil phase is shinyleaf yellowhorn oil, and water is mainly Wood of Shinyleaf Yellowhorn protolysate peptide, and solid phase is that the unhydrolysed residue of Wood of Shinyleaf Yellowhorn contains part grease, albumen, Mierocrystalline cellulose.Unsaturated fatty acid content is higher than 93% in the shinyleaf yellowhorn oil that obtains;
7) with 6) communicated the earlier ultra-filtration membrane ultrafiltration and concentration of molecular weight 4000-6000 of the described solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains macromole Wood of Shinyleaf Yellowhorn polysaccharide content about 10-20%, and then removes residual sugar and be concentrated into protein peptide content about 15-20% by the nanofiltration of molecular weight 300-500;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 12-15MPa, inlet temperature: 155-185 ℃, temperature of outgoing air: 75-85 ℃, the Wood of Shinyleaf Yellowhorn polysaccharide molecular-weight average 10-15 ten thousand that obtains after the drying, water content is less than 5%, and purity is greater than more than 90%;
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 12-15MPa, inlet temperature: 155-185 ℃, temperature of outgoing air: 75-85 ℃, the protein peptide powder water content that obtains after the drying is less than 5%, and purity is greater than more than 85%;
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
The invention provides a kind of fine shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn proteolysis peptide powder, it can be widely used in industries such as food and healthcare products.Its concrete advantage is as follows: 1, product of the present invention is by being rich in grease and proteic civil official's kernel through the refining all-natural product that forms of scientific approach, using any organic solvent in making processes, belonging to pure natural, high nutrition, health food.2, pure natural shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and proteolysis peptide powder have very good market comsupton prospect, can be used as the batching of food or raw materials for food industry and medicine and healthcare products etc., be widely used in beverage, cold drink, candy, protective foods, bake, industries such as snackfoods, medicine, preparation becomes various nutrition and functional food and medicine with deep processing.Its product has very large market capacity, and its economic and social benefits also will increase substantially thereupon.3, preparation technology of the present invention adopts the microwave pre-treatment, extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, and Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide powder have improved extract yield, have reduced extraction time, and have not had waste discharge, reduce cost, and increase economic efficiency.
In a word: the present invention overcomes the various shortcoming that traditional vegetables oil reparation technology brings, and adopts brand-new technology, and a kind of not only pollution-free to greatest extent extraction shinyleaf yellowhorn oil is provided, and obtains Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide simultaneously.All the course of processing has embodied continuity, quick and mechanize, has improved production efficiency greatly.
Embodiment
Embodiment 1, and a kind of method of extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn may further comprise the steps:
1) Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds Wood of Shinyleaf Yellowhorn benevolence weight 20% simultaneously stirs, and places and makes its swelling in 2 hours;
2) civil official's kernel swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and handle, the frequency of microwave is 2400 MHz, and power is 60KW, and 110 ℃ of temperature, transfer rate are 2.0m/min, and the time is 3.0 min;
3) civil official's kernel of microwave treatment is added 5 times of water of its weight, regulate pH value to 8.5, add 1% Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value 8.5 under 50 ℃ of conditions, hydrolysis 3 hours;
4) then under 7.0,50 ℃ of conditions of pH value, each adds 0.3% neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 4 hours of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 90 ℃, stirs 20min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains oil phase, water and unhydrolysed residue solid phase respectively.Oil phase is shinyleaf yellowhorn oil, and water is mainly Wood of Shinyleaf Yellowhorn protolysate peptide, and solid phase is that the unhydrolysed residue of Wood of Shinyleaf Yellowhorn contains part grease, albumen, Mierocrystalline cellulose.Unsaturated fatty acid content 94% in the shinyleaf yellowhorn oil that obtains.Wherein linolic acid accounts for 37% oleic acid and accounts for 57%;
7) with 6) the described solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains macromole Wood of Shinyleaf Yellowhorn polysaccharide content about 10% by the ultra-filtration membrane ultrafiltration and concentration of molecular weight 4000, and then removes residual sugar and be concentrated into protein peptide content about 15% by the nanofiltration of molecular weight 300;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 15 MPa, inlet temperature: 185 ℃, temperature of outgoing air: 85 ℃, the Wood of Shinyleaf Yellowhorn polysaccharide molecular-weight average 100,000 that obtains after the drying, water content 5%, purity 91%.
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 12 MPa, and inlet temperature: 155 ℃, temperature of outgoing air: 85 ℃, the protein peptide powder water content 4% that obtains after the drying, purity 88%.
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
Embodiment 2
1) Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds Wood of Shinyleaf Yellowhorn benevolence weight 30% simultaneously stirs, and places and makes its swelling in 1 hour;
2) civil official's kernel swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and handle, the frequency of microwave is 2500MHz, and power is 60KW, and 121 ℃ of temperature, transfer rate are 4.0m/min, and the time is 1.5 min.
3) civil official's kernel of microwave treatment is added 5 times of water of its weight, regulate pH value to 9.0, add 2% Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value 9.0 under 55 ℃ of conditions, hydrolysis 2 hours;
4) then under 7.0,55 ℃ of conditions of pH value, each adds 0.5% neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 2 hours of Wood of Shinyleaf Yellowhorn benevolence weight.
5) with 4) the described liquid that obtains is warming up to 95 ℃, stirs 10min go out enzyme, breakdown of emulsion.
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains oil phase, water and unhydrolysed residue solid phase respectively.Oil phase is shinyleaf yellowhorn oil, and water is mainly Wood of Shinyleaf Yellowhorn protolysate peptide, and solid phase is that the unhydrolysed residue of Wood of Shinyleaf Yellowhorn contains part grease, albumen, Mierocrystalline cellulose.Unsaturated fatty acid content 95% in the shinyleaf yellowhorn oil that obtains.Wherein linolic acid accounts for 37.% oleic acid and accounts for 58%.
7) with 6) the described solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains macromole Wood of Shinyleaf Yellowhorn polysaccharide content about 20% by the ultra-filtration membrane ultrafiltration and concentration of molecular weight 6000, and removing residual sugar by the nanofiltration of molecular weight 500 and be concentrated into protein peptide content about 20% then.
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 13 MPa, inlet temperature: 175 ℃, temperature of outgoing air: 75 ℃, the Wood of Shinyleaf Yellowhorn polysaccharide molecular-weight average 150,000 that obtains after the drying, water content 3%, purity 93%.
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 14MPa, and inlet temperature: 165 ℃, temperature of outgoing air: 75 ℃, the protein peptide powder water content 3.5% that obtains after the drying, purity 86%.
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
Embodiment 3, a kind of method of extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn may further comprise the steps:
1) get the benevolence of Wood of Shinyleaf Yellowhorn, Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds Wood of Shinyleaf Yellowhorn benevolence weight 25% simultaneously stirs, and places and makes its swelling in 1.5 hours;
2) the Wood of Shinyleaf Yellowhorn benevolence swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and handle, the frequency of microwave is 2450MHz, and power is 60KW, and 110 ℃ of temperature, transfer rate are 3.0m/min, and the time is 2.0 min;
3) the Wood of Shinyleaf Yellowhorn benevolence of microwave treatment is added 5.5 times of water of its weight, regulate pH value to 9, add 1.5% Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value 9 under 50 ℃ of conditions, hydrolysis 2.5 hours;
4) then at pH value 6.5-7.0, under 50 ℃ of conditions, each adds 0.4% neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 3 hours of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 90 ℃, stirs 15min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains oil phase, water and unhydrolysed residue solid phase respectively.Oil phase is shinyleaf yellowhorn oil, and water is mainly Wood of Shinyleaf Yellowhorn protolysate peptide, and solid phase is that the unhydrolysed residue of Wood of Shinyleaf Yellowhorn contains part grease, albumen, Mierocrystalline cellulose.Unsaturated fatty acid content is higher than 93% in the shinyleaf yellowhorn oil that obtains;
7) with 6) communicated the earlier ultra-filtration membrane ultrafiltration and concentration of molecular weight 5000 of the described solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains macromole Wood of Shinyleaf Yellowhorn polysaccharide content about 10-20%, and then removes residual sugar and be concentrated into protein peptide content about 15-20% by the nanofiltration of molecular weight 400;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 13MPa, inlet temperature: 170 ℃, temperature of outgoing air: 80 ℃, the Wood of Shinyleaf Yellowhorn polysaccharide molecular-weight average 140,000 that obtains after the drying, water content is less than 4.5%, and purity is greater than more than 95%;
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 14MPa, and inlet temperature: 170 ℃, temperature of outgoing air: 80 ℃, the protein peptide powder water content that obtains after the drying is less than 3%, and purity is greater than more than 90%;
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
Claims (2)
1. method of extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn is characterized in that: may further comprise the steps:
1) Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds Wood of Shinyleaf Yellowhorn benevolence weight 20-30% simultaneously stirs, and places and makes its swelling in 1-2 hour;
2) the Wood of Shinyleaf Yellowhorn benevolence swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and handle, the frequency of microwave is 2400-2500MHz, and power is 60KW, 100 ℃-121 ℃ of temperature, transfer rate is 2.0-4.0m/min, and the time is 1.5-3.0 min;
3) the Wood of Shinyleaf Yellowhorn benevolence after the microwave treatment is added 5-6 times of water of its weight, regulate pH value, add the Sumizyme MP of the 1-2% of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value at 8.5-9.5 under the 45-60 ℃ of condition, hydrolysis 2-3 hour to 8.5-9.5;
4) then at pH value 6.5-7.0, under the 45-60 ℃ of condition, each adds neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 2-4 hour of the 0.3-0.5% of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 80-100 ℃, stirs 10-20min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains oil phase, water and unhydrolysed residue solid phase respectively;
7) with 6) communicated the earlier ultra-filtration membrane ultrafiltration and concentration of molecular weight 4000-6000 of the described aqueous phase solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains the Wood of Shinyleaf Yellowhorn polysaccharide soln, and then removes residual sugar and be concentrated into protein peptide content at 15-20% by the nanofiltration of molecular weight 300-500;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, and its condition is: high-pressure pump pressure-controlling 12-15MPa, inlet temperature: 155-185 ℃, temperature of outgoing air: 75-85 ℃;
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, and its condition is: high-pressure pump pressure-controlling 12-15MPa, inlet temperature: 155-185 ℃, temperature of outgoing air: 75-85 ℃;
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
2. the method for extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn according to claim 1 is characterized in that: may further comprise the steps:
1) Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds Wood of Shinyleaf Yellowhorn benevolence weight 25% simultaneously stirs, and places and makes its swelling in 1.5 hours;
2) the Wood of Shinyleaf Yellowhorn benevolence swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and handle, the frequency of microwave is 2450MHz, and power is 60KW, and 110 ℃ of temperature, transfer rate are 3.0m/min, and the time is 2.0 min;
3) the Wood of Shinyleaf Yellowhorn benevolence after the microwave treatment is added 5.5 times of water of its weight, regulate pH value to 9, add 1.5% Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value 9 under 50 ℃ of conditions, hydrolysis 2.5 hours;
4) then at pH value 6.5-7.0, under 50 ℃ of conditions, each adds 0.4% neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 3 hours of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 90 ℃, stirs 15min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains oil phase, water and unhydrolysed residue solid phase respectively;
7) with 6) communicated the earlier ultra-filtration membrane ultrafiltration and concentration of molecular weight 500 of the described aqueous phase solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains the Wood of Shinyleaf Yellowhorn polysaccharide soln, and then removes residual sugar and be concentrated into protein peptide content at 15-20% by the nanofiltration of molecular weight 400;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, and its condition is: high-pressure pump pressure-controlling 13MPa, inlet temperature: 170 ℃, temperature of outgoing air: 80 ℃;
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, and its condition is: high-pressure pump pressure-controlling 14MPa, inlet temperature: 170 ℃, temperature of outgoing air: 80 ℃;
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
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