CN104788535A - Method for simultaneously preparing shinyleaf yellowhorn oil and shinyleaf yellowhorn protein with aqueous enzymatic method - Google Patents
Method for simultaneously preparing shinyleaf yellowhorn oil and shinyleaf yellowhorn protein with aqueous enzymatic method Download PDFInfo
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Abstract
The invention discloses a method for simultaneously preparing shinyleaf yellowhorn oil and shinyleaf yellowhorn protein with an aqueous enzymatic method. The method comprises the following steps: 1, obtaining pulp; 2, adding a polysaccharide complex enzyme for enzymolysis, and obtaining primary shinyleaf yellowhorn oil; 3, performing primary emulsion breaking, and obtaining secondary shinyleaf yellowhorn oil; 4, performing secondary emulsion breaking, obtaining tertiary shinyleaf yellowhorn oil, uniformly mixing the tertiary shinyleaf yellowhorn oil with the primary shinyleaf yellowhorn oil and the secondary shinyleaf yellowhorn oil, and obtaining the shinyleaf yellowhorn oil; 5, performing primary alkali extraction; 6, performing secondary alkali extraction; 7, performing acid precipitation, and obtaining the shinyleaf yellowhorn protein. The method for simultaneously preparing the shinyleaf yellowhorn oil and the shinyleaf yellowhorn protein with the aqueous enzymatic method is simple and safe in process; the extracted shinyleaf yellowhorn oil is richer in fragrance, light in color and good in quality; the prepared shinyleaf yellowhorn protein is higher in purity and can serve as a good functional ingredient to be widely applied to food processing, and the protein content is higher than 50%.
Description
Technical field
The present invention relates to agricultural-food intensive processing and byproduct comprehensive utilizes technical field, be specifically related to a kind of method that aqueous enzymatic method prepares shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously.
Background technology
Wood of Shinyleaf Yellowhorn has higher industrial value and nutritive value, lubricant component content in seed and kind benevolence is high, result of study proves: plant oleaginousness in benevolence and reach 55 ~ 67%, in the kind benevolence of improved seeds, oleaginousness reaches 72%, exceed general oilseed plant, the essentially consist of its grease is as follows: one of major ingredient of stearic acid, the general edible oil of oleic acid 38.9%(), linolic acid 40.2%(and soya-bean oil, walnut oil be close, is also the part that nutritive value is the highest), behenic acid 7.2%, linolenic acid and sweet carbon enoic acid be respectively 0.3%.Wood of Shinyleaf Yellowhorn is the distinctive economic woody oil tree species of China, seed oil length is 30.4% ~ 47%, plant benevolence oleaginousness up to 66.39%, oil yellow look and transparent, edible delicious, in oil, contained linolic acid is the major ingredient of Chinese medicine linoleic acid, has fabulous hypotensive activity, and edible shinyleaf yellowhorn oil can the effective illness such as preventing hypertension, hyperlipidemia, arteriosclerosis.
The emulsifying property of Wood of Shinyleaf Yellowhorn albumen is better, and Wood of Shinyleaf Yellowhorn albumen can be used as emulsifying agent, adds in baked food, frozen product and soups food, to improve the stability of goods.Wood of Shinyleaf Yellowhorn Argine Monohydrochloride rich content, reaches 21.68%, and Wood of Shinyleaf Yellowhorn albumen is the good protein of mass ratio.According to WHO suggestion, with the amino acid contained ratio of egg protein for reference standard, when not detecting tryptophane, the amino acid evaluation score of Wood of Shinyleaf Yellowhorn albumen is better than Semen arachidis hypogaeae protein.Therefore, be necessary to study a kind of method simultaneously preparing shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen.
Summary of the invention
For the weak point existed in above-mentioned technology, the invention provides a kind of method that aqueous enzymatic method prepares shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, its technique is simple and safe and make the shinyleaf yellowhorn oil fragrance of extraction denseer, and Wood of Shinyleaf Yellowhorn purity of protein is higher.
The technical solution adopted for the present invention to solve the technical problems is: a kind of aqueous enzymatic method prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, comprises the steps: that step one, slurries obtain: by Wood of Shinyleaf Yellowhorn benevolence raw material and water mixing, obtained slurries; Step 2, enzymolysis: after regulating slurries pH, add polysaccharide composite enzyme enzymolysis, treat that enzyme digestion reaction terminates, enzymolysis solution is centrifugal, obtain the first layer edible vegetable oil, second layer emulsion layer, third layer waste liquid and the 4th layer of crude protein successively, successively draw and obtain shinyleaf yellowhorn oil, an emulsion layer and a crude protein liquid; Step 3, a breakdown of emulsion: emulsion layer be separated, the freezing breakdown of emulsion that thaws again, obtain an emulsion and it is centrifugal, obtains the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, successively draw and obtain secondary shinyleaf yellowhorn oil and second emulsifying layer; Step 4, secondary breakdown of emulsion: second emulsifying layer is separated, the freezing breakdown of emulsion that thaws again, obtain secondary emulsion and it is centrifugal, obtain the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, draw upper strata edible vegetable oil and obtain three shinyleaf yellowhorn oils, and mix with a shinyleaf yellowhorn oil and secondary shinyleaf yellowhorn oil, obtained shinyleaf yellowhorn oil; Step 5, an alkali are carried: a crude protein liquid is carried out an alkali and carries, and obtain an alkali extract and it is centrifugal, obtain upper strata protein solution and lower sediment, are isolated and obtain a protein liquid and primary sedimentation; Step 6, secondary alkali are carried: primary sedimentation is carried out secondary alkali and carries, and obtain secondary alkali extract and it is centrifugal, obtain upper strata protein solution and lower sediment, are isolated and obtain secondary protein liquid; Step 7, acid are sunk: by a protein liquid and the mixing of secondary protein liquid, carry out acid heavy centrifugal, be precipitated powder, carry out lyophilize to precipitated powder, obtain Wood of Shinyleaf Yellowhorn albumen after regulating pH.
Preferably, the Wood of Shinyleaf Yellowhorn benevolence stock preparation process in described step one is: decortication of being shelled by Wood of Shinyleaf Yellowhorn obtains pure Wood of Shinyleaf Yellowhorn benevolence, pure Wood of Shinyleaf Yellowhorn benevolence is pulverized and sieved, and obtaining granular size is 40 ~ 80 object Wood of Shinyleaf Yellowhorn benevolence raw materials.
Preferably, the slurries in described step one mix obtained by Wood of Shinyleaf Yellowhorn benevolence raw material and water being pressed solid-liquid ratio 1g:4 ~ 6mL.
Preferably, in slurries, hydrochloric acid is added or slurries pH is adjusted to 4 ~ 6 by citric acid in described step 2, add polysaccharide composite enzyme enzymolysis, enzyme digestion reaction temperature is 45 ~ 55 DEG C, the enzyme digestion reaction time is 2 ~ 6 hours, treat that enzyme digestion reaction terminates, enzymolysis solution is warming up to 75 ~ 85 DEG C of enzymes 15 ~ 25 minutes of going out, centrifugal after regulating enzymolysis solution pH to 4 ~ 5.
Preferably, the polysaccharide composite enzyme in described step 2 adopts cellulase+polygalacturonase, and the consumption of described cellulase and polygalacturonase is 1:0.5 ~ 10, and the enzyme concentration of polysaccharide composite enzyme is 500 ~ 2000u/g of Wood of Shinyleaf Yellowhorn benevolence raw material dosage.
Preferably, a described breakdown of emulsion and secondary breakdown of emulsion condition are: freezing temp-25 DEG C, freezing time 24 hours, and thaw point 40 DEG C thaws 4 hours.
Preferably, the condition that in described step 5, alkali is carried is: alkali carries solid-liquid ratio 1:5 ~ 10, alkali temperature raising degree 50 ~ 60 DEG C, and alkali carries pH8 ~ 10, and alkali carries 1 ~ 3 hour time.
Preferably, the condition that in described step 6, secondary alkali is carried is: alkali carries solid-liquid ratio 1:10 ~ 15, alkali temperature raising degree 50-60 DEG C, and alkali carries pH8-10, and alkali carries 0.5 ~ 2 hour time.
Preferably, by a protein liquid and the mixing of secondary protein liquid in described step 7, add hydrochloric acid or lemon acid for adjusting pH to 4.6 and carry out acid and sink, it is 40 DEG C that temperature is sunk in acid, centrifugal after floss to appear.
Compared with prior art, its beneficial effect is in the present invention:
(1) the present invention adopts polysaccharide composite enzyme enzymolysis, and destroy cellularstructure and grease is disengaged, the oil yield compared to single use cellulase or pectinase enzymatic hydrolysis is higher; Compare use protease hydrolyzed, cellulase in polysaccharide composite enzyme and the optimal pH of polygalacturonase all fall in acid condition, therefore only need to carry out primary enzymolysis, and when using protease hydrolyzed, due to reaction conditions difference, proteolytic enzyme mostly is alkalescence or neutral, therefore needs to carry out twice enzymolysis, increases enzyme digestion reaction time 2 ~ 5 hours; In addition, when using protease hydrolyzed, easy is polypeptide by proteolysis, reduce the yield of albumen, and the protein product purity obtained is lower, and when using polysaccharide composite enzyme enzymolysis, and by crude protein liquid that breakdown of emulsion alkali extraction and acid precipitation obtains, albumen yield can be made to rise to 40% and purity rising, considerably increase the yield of albumen.
(2) due to can milk sap be formed when aqueous enzymatic method carries oil, its composition is oil, water, a small amount of protein, starch and Mierocrystalline cellulose, compared to heating demulsification type, microwave exposure and breakdown of emulsion of saltouing, the double freeze-thaw breakdown of emulsion that the present invention adopts better can destroy the stability of milk sap, owing to there will be Crystallization in Droplets of O in milk sap in refrigerating process, and these adipic crystals can thrust aqueous phase, when these adipic crystals appear between adjacent oil droplet just, to interfacial film be pierced through cause the gathering of oil droplet, thus significantly reduce the stability of milk sap, reach the object of breakdown of emulsion, the present invention adopts double freeze-thaw breakdown of emulsion method to substantially increase oil yield, oil yield is from about 20% before breakdown of emulsion, be provided to the highest 40%, fully be extracted the grease in Wood of Shinyleaf Yellowhorn.
(3) the present invention extracts Wood of Shinyleaf Yellowhorn albumen by adopting double alkali extraction and acid precipitation, and the condition that change twice, front and back alkali is carried, by increasing the solid-liquid ratio that secondary alkali is carried, the dissolving sucking-off of Wood of Shinyleaf Yellowhorn albumen effectively can be promoted; Carry solid-liquid ratio due to secondary alkali to increase, and time corresponding minimizing, and secondary alkali carries the later stage, the stripping increasing amount reduction in time of protein, therefore by changing the time that twice, front and back alkali is carried, equipment can be made to be fully used, improve production efficiency.
(4) aqueous enzymatic method provided by the invention prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, and technique is simple and safe, the shinyleaf yellowhorn oil thick flavor of extraction, and of light color, quality is good; Obtained Wood of Shinyleaf Yellowhorn purity of protein is higher, and protein content, higher than 50%, can be used as a kind of good functional ingredient and is widely used in food-processing.
Accompanying drawing explanation
Fig. 1 is the method process flow sheet that aqueous enzymatic method of the present invention prepares shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, can implement according to this with reference to specification sheets word to make those skilled in the art.
As shown in Figure 1, the invention provides a kind of method that aqueous enzymatic method prepares shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, comprise the steps:
Step one, slurries obtain: decortication of being shelled by Wood of Shinyleaf Yellowhorn obtains pure Wood of Shinyleaf Yellowhorn benevolence, pure Wood of Shinyleaf Yellowhorn benevolence is pulverized and sieved, obtaining granular size is 40 ~ 80 object Wood of Shinyleaf Yellowhorn benevolence raw materials, reduces the granular size of Wood of Shinyleaf Yellowhorn benevolence raw material, increases the specific surface area of molecule, enzymolysis contact area can be increased, thus better promote reaction, be conducive to fuel-displaced, Wood of Shinyleaf Yellowhorn benevolence raw material and water pressed solid-liquid ratio 1g:4 ~ 6mL and mix, obtained slurries, can ensure within the scope of this solid-liquid ratio that enzyme-to-substrate concentration ratio is suitable for;
Step 2, enzymolysis: add hydrochloric acid or slurries pH is adjusted to 4 ~ 6 by citric acid in slurries, add polysaccharide composite enzyme enzymolysis, polysaccharide composite enzyme adopts cellulase+polygalacturonase, the optimal pH of cellulase and polygalacturonase is all between 4 ~ 6, optimum temperuture is all at 45-55 DEG C, the consumption of described cellulase and polygalacturonase is 1:0.5 ~ 10, and the enzyme concentration of polysaccharide composite enzyme is 500 ~ 2000u/g of Wood of Shinyleaf Yellowhorn benevolence raw material dosage, enzyme digestion reaction temperature is 45 ~ 55 DEG C, the enzyme digestion reaction time is 2 ~ 6 hours, treat that enzyme digestion reaction terminates, enzymolysis solution is warming up to 75 ~ 85 DEG C of enzymes 15 ~ 25 minutes of going out, centrifugal after regulating enzymolysis solution pH to 4 ~ 5, centrifugal condition is: rotating speed 4000 ~ 5500r/min, centrifugal 10 ~ 30min, obtain the first layer edible vegetable oil successively, second layer emulsion layer, third layer waste liquid and the 4th layer of crude protein, successively draw and obtain a shinyleaf yellowhorn oil, an emulsion layer and a crude protein liquid,
Step 3, a breakdown of emulsion: an emulsion layer is separated, the freezing breakdown of emulsion that thaws again, one time breakdown of emulsion condition is: freezing temp-25 DEG C, freezing time 24 hours, thaw point 40 DEG C, thaw 4 hours, obtain an emulsion and it is centrifugal, centrifugal condition is: rotating speed 4000 ~ 5500r/min, centrifugal 10 ~ 30min, obtain the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, successively draw and obtain secondary shinyleaf yellowhorn oil and second emulsifying layer;
Step 4, secondary breakdown of emulsion: second emulsifying layer is separated, the freezing breakdown of emulsion that thaws again, described secondary breakdown of emulsion condition is: freezing temp-25 DEG C, freezing time 24 hours, thaw point 40 DEG C, thaw 4 hours, obtain secondary emulsion and it is centrifugal, centrifugal condition is: rotating speed 4000 ~ 5500r/min, centrifugal 10 ~ 30min, obtains the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, draws upper strata edible vegetable oil and obtains three shinyleaf yellowhorn oils, and mix with a shinyleaf yellowhorn oil and secondary shinyleaf yellowhorn oil, obtained shinyleaf yellowhorn oil;
Step 5, an alkali are carried: a crude protein liquid is carried out an alkali and carries, under alkaline environment, protein can fully dissolve, the condition that alkali is carried is: alkali carries solid-liquid ratio 1:5 ~ 10, alkali temperature raising degree 50 ~ 60 DEG C, and alkali carries pH8 ~ 10, alkali carries 1 ~ 3 hour time, obtain an alkali extract and it is centrifugal, centrifugal condition is: rotating speed 4000 ~ 5500r/min, centrifugal 10 ~ 30min, obtain upper strata protein solution and lower sediment, be isolated and obtain a protein liquid and primary sedimentation;
Step 6, secondary alkali is carried: primary sedimentation is carried out secondary alkali and carries, secondary alkali carry object be guarantee alkali when carrying undissolved protein can fully dissolve, the condition that secondary alkali is carried is: alkali carries solid-liquid ratio 1:10 ~ 15, alkali temperature raising degree 50-60 DEG C, alkali carries pH8-10, alkali carries 0.5 ~ 2 hour time, obtain secondary alkali extract and it is centrifugal, centrifugal condition is: rotating speed 4000 ~ 5500r/min, centrifugal 10 ~ 30min, obtain upper strata protein solution and lower sediment, be isolated and obtain secondary protein liquid, increasing solid-liquid ratio when secondary alkali is carried is to make protein dissolve more fully, increase the yield of protein,
Step 7, acid are sunk: by a protein liquid and the mixing of secondary protein liquid, add hydrochloric acid or lemon acid for adjusting pH to 4.6 and carry out acid heavy (Wood of Shinyleaf Yellowhorn albumen iso-electric point is 4.6), the heavy temperature of acid is 40 DEG C, centrifugal after floss to appear, centrifugal condition is: rotating speed 4000 ~ 5500r/min, and centrifugal 10 ~ 30min, is precipitated powder, lyophilize is carried out to precipitated powder, obtains Wood of Shinyleaf Yellowhorn albumen.
Compared with prior art, its beneficial effect is in the present invention: the present invention adopts polysaccharide composite enzyme enzymolysis, and destroy cellularstructure and grease is disengaged, the oil yield compared to single use cellulase or pectinase enzymatic hydrolysis is higher; Compare use protease hydrolyzed, cellulase in polysaccharide composite enzyme and the optimal pH of polygalacturonase all fall in acid condition, therefore only need to carry out primary enzymolysis, and when using protease hydrolyzed, due to reaction conditions difference, proteolytic enzyme mostly is alkalescence or neutral, therefore needs to carry out twice enzymolysis, increases enzyme digestion reaction time 2 ~ 5 hours; In addition, when using protease hydrolyzed, easy is polypeptide by proteolysis, reduce the yield of albumen, and the protein product purity obtained is lower, and when using polysaccharide composite enzyme enzymolysis, and by crude protein liquid that breakdown of emulsion alkali extraction and acid precipitation obtains, albumen yield can be made to rise to 40% and purity rising, considerably increase the yield of albumen.Due to can milk sap be formed when aqueous enzymatic method carries oil, its composition is oil, water, a small amount of protein, starch and Mierocrystalline cellulose, compared to heating demulsification type, microwave exposure and breakdown of emulsion of saltouing, the double freeze-thaw breakdown of emulsion that the present invention adopts better can destroy the stability of milk sap, owing to there will be Crystallization in Droplets of O in milk sap in refrigerating process, and these adipic crystals can thrust aqueous phase, when these adipic crystals appear between adjacent oil droplet just, to interfacial film be pierced through cause the gathering of oil droplet, thus significantly reduce the stability of milk sap, reach the object of breakdown of emulsion, the present invention adopts double freeze-thaw breakdown of emulsion method to substantially increase oil yield, oil yield is from about 20% before breakdown of emulsion, be provided to the highest 40%, fully be extracted the grease in Wood of Shinyleaf Yellowhorn.The present invention extracts Wood of Shinyleaf Yellowhorn albumen by adopting double alkali extraction and acid precipitation, and the condition that change twice, front and back alkali is carried, by increasing the solid-liquid ratio that secondary alkali is carried, the dissolving sucking-off of Wood of Shinyleaf Yellowhorn albumen effectively can be promoted; Carry solid-liquid ratio due to secondary alkali to increase, and time corresponding minimizing, and secondary alkali carries the later stage, the stripping increasing amount reduction in time of protein, therefore by changing the time that twice, front and back alkali is carried, equipment can be made to be fully used, improve production efficiency.Aqueous enzymatic method provided by the invention prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, and technique is simple and safe, the shinyleaf yellowhorn oil thick flavor of extraction, and of light color, quality is good; Obtained Wood of Shinyleaf Yellowhorn purity of protein is higher, and protein content, higher than 50%, can be used as a kind of good functional ingredient and is widely used in food-processing.
Embodiment 1:
Decortication of being shelled by Wood of Shinyleaf Yellowhorn obtains pure Wood of Shinyleaf Yellowhorn benevolence, pure Wood of Shinyleaf Yellowhorn benevolence is pulverized and sieved, and obtaining granular size is 60 object Wood of Shinyleaf Yellowhorn benevolence raw materials, Wood of Shinyleaf Yellowhorn benevolence raw material and water is pressed solid-liquid ratio 1g:5mL and mixes, obtained slurries, in slurries, add hydrochloric acid slurries pH is adjusted to 5, add polysaccharide composite enzyme enzymolysis, polysaccharide composite enzyme adopts cellulase+polygalacturonase, the consumption of described cellulase and polygalacturonase is 1:1, and the enzyme concentration of polysaccharide composite enzyme is the 1000u/g of Wood of Shinyleaf Yellowhorn benevolence raw material dosage, enzyme digestion reaction temperature is 50 DEG C, the enzyme digestion reaction time is 3 hours, treat that enzyme digestion reaction terminates, enzymolysis solution is warming up to 80 DEG C of enzymes 20 minutes of going out, centrifugal after regulating enzymolysis solution pH to 4.6, centrifugal condition is: rotating speed 5500r/min, centrifugal 10min, obtain the first layer edible vegetable oil successively, second layer emulsion layer, third layer waste liquid and the 4th layer of crude protein, successively draw and obtain a shinyleaf yellowhorn oil, an emulsion layer and a crude protein liquid, an emulsion layer is separated, the freezing breakdown of emulsion that thaws again, one time breakdown of emulsion condition is: freezing temp-25 DEG C, freezing time 24 hours, thaw point 40 DEG C, thaw 4 hours, obtain an emulsion and it is centrifugal, centrifugal condition is: rotating speed 4000/min, centrifugal 10min, obtain the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, successively draw and obtain secondary shinyleaf yellowhorn oil and second emulsifying layer, be separated by second emulsifying layer, the freezing breakdown of emulsion that thaws again, described secondary breakdown of emulsion condition is: freezing temp-25 DEG C, freezing time 24 hours, thaw point 40 DEG C, thaws 4 hours, obtains secondary emulsion and it is centrifugal, centrifugal condition is: rotating speed 4000r/min, centrifugal 10min, obtains the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, draws upper strata edible vegetable oil and obtains three shinyleaf yellowhorn oils, and mix with a shinyleaf yellowhorn oil and secondary shinyleaf yellowhorn oil, obtained shinyleaf yellowhorn oil, a crude protein liquid is carried out an alkali carry, the condition that alkali is carried is: alkali carries solid-liquid ratio 1:5, alkali carries temperature 60 C, alkali carries pH9, and alkali carries 3 hours time, obtains an alkali extract and it is centrifugal, centrifugal condition is: rotating speed 4000r/min, centrifugal 10min, obtains upper strata protein solution and lower sediment, is isolated and obtains a protein liquid and primary sedimentation, primary sedimentation is carried out secondary alkali carry, the condition that secondary alkali is carried is: alkali carries solid-liquid ratio 1:10, alkali carries temperature 60 C, alkali carries pH9, and alkali carries 1 hour time, obtains secondary alkali extract and it is centrifugal, centrifugal condition is: rotating speed 4000r/min, centrifugal 10min, obtains upper strata protein solution and lower sediment, is isolated and obtains secondary protein liquid, by a protein liquid and the mixing of secondary protein liquid, add hydrochloric acid or lemon acid for adjusting pH to 4.6 to carry out acid and sink, the heavy temperature of acid is 40 DEG C, centrifugal after floss to appear, centrifugal condition is: rotating speed 4000r/min, and centrifugal 10min, is precipitated powder, lyophilize is carried out to precipitated powder, obtains Wood of Shinyleaf Yellowhorn albumen, shinyleaf yellowhorn oil yield 35.6%, extraction yield 61.4%, Wood of Shinyleaf Yellowhorn purity of protein (butt) 51.6%, extraction yield 40.8%.Wherein, Wood of Shinyleaf Yellowhorn benevolence oil length is 58.2%, and protein content is 29.7%.
Embodiment 2:
Decortication of being shelled by Wood of Shinyleaf Yellowhorn obtains pure Wood of Shinyleaf Yellowhorn benevolence, pure Wood of Shinyleaf Yellowhorn benevolence is pulverized and sieved, and obtaining granular size is 60 object Wood of Shinyleaf Yellowhorn benevolence raw materials, Wood of Shinyleaf Yellowhorn benevolence raw material and water is pressed solid-liquid ratio 1g:5mL and mixes, obtained slurries, in slurries, add hydrochloric acid slurries pH is adjusted to 5, add polysaccharide composite enzyme enzymolysis, polysaccharide composite enzyme adopts cellulase+polygalacturonase, the consumption of described cellulase and polygalacturonase is 1:10, and the enzyme concentration of polysaccharide composite enzyme is the 2000u/g of Wood of Shinyleaf Yellowhorn benevolence raw material dosage, enzyme digestion reaction temperature is 50 DEG C, the enzyme digestion reaction time is 5 hours, treat that enzyme digestion reaction terminates, enzymolysis solution is warming up to 80 DEG C of enzymes 20 minutes of going out, centrifugal after regulating enzymolysis solution pH to 4.6, centrifugal condition is: rotating speed 5500r/min, centrifugal 10min, obtain the first layer edible vegetable oil successively, second layer emulsion layer, third layer waste liquid and the 4th layer of crude protein, successively draw and obtain a shinyleaf yellowhorn oil, an emulsion layer and a crude protein liquid, an emulsion layer is separated, the freezing breakdown of emulsion that thaws again, one time breakdown of emulsion condition is: freezing temp-25 DEG C, freezing time 24 hours, thaw point 40 DEG C, thaw 4 hours, obtain an emulsion and it is centrifugal, centrifugal condition is: rotating speed 4000/min, centrifugal 10min, obtain the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, successively draw and obtain secondary shinyleaf yellowhorn oil and second emulsifying layer, be separated by second emulsifying layer, the freezing breakdown of emulsion that thaws again, described secondary breakdown of emulsion condition is: freezing temp-25 DEG C, freezing time 24 hours, thaw point 40 DEG C, thaws 4 hours, obtains secondary emulsion and it is centrifugal, centrifugal condition is: rotating speed 4000r/min, centrifugal 10min, obtains the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, draws upper strata edible vegetable oil and obtains three shinyleaf yellowhorn oils, and mix with a shinyleaf yellowhorn oil and secondary shinyleaf yellowhorn oil, obtained shinyleaf yellowhorn oil, a crude protein liquid is carried out an alkali carry, the condition that alkali is carried is: alkali carries solid-liquid ratio 1:5, alkali carries temperature 60 C, alkali carries pH9, and alkali carries 3 hours time, obtains an alkali extract and it is centrifugal, centrifugal condition is: rotating speed 4000r/min, centrifugal 10min, obtains upper strata protein solution and lower sediment, is isolated and obtains a protein liquid and primary sedimentation, primary sedimentation is carried out secondary alkali carry, the condition that secondary alkali is carried is: alkali carries solid-liquid ratio 1:15, alkali carries temperature 60 C, alkali carries pH9, and alkali carries time 2 h, obtains secondary alkali extract and it is centrifugal, centrifugal condition is: rotating speed 4000r/min, centrifugal 10min, obtains upper strata protein solution and lower sediment, is isolated and obtains secondary protein liquid, by a protein liquid and the mixing of secondary protein liquid, add hydrochloric acid or lemon acid for adjusting pH to 4.6 to carry out acid and sink, the heavy temperature of acid is 40 DEG C, centrifugal after floss to appear, centrifugal condition is: rotating speed 4000r/min, and centrifugal 10min, is precipitated powder, lyophilize is carried out to precipitated powder, obtains Wood of Shinyleaf Yellowhorn albumen, shinyleaf yellowhorn oil yield 41.6%, extraction yield 75.4%, Wood of Shinyleaf Yellowhorn purity of protein (butt) 55.9%, extraction yield 48.1%.Wherein, Wood of Shinyleaf Yellowhorn benevolence oil length is 55.1%, and protein content is 29.9%.
Embodiment 3:
Decortication of being shelled by Wood of Shinyleaf Yellowhorn obtains pure Wood of Shinyleaf Yellowhorn benevolence, pure Wood of Shinyleaf Yellowhorn benevolence is pulverized and sieved, and obtaining granular size is 60 object Wood of Shinyleaf Yellowhorn benevolence raw materials, Wood of Shinyleaf Yellowhorn benevolence raw material and water is pressed solid-liquid ratio 1g:5mL and mixes, obtained slurries, in slurries, add hydrochloric acid slurries pH is adjusted to 5, add polysaccharide composite enzyme enzymolysis, polysaccharide composite enzyme adopts cellulase+polygalacturonase, the consumption of described cellulase and polygalacturonase is 1:2, and the enzyme concentration of polysaccharide composite enzyme is the 1000u/g of Wood of Shinyleaf Yellowhorn benevolence raw material dosage, enzyme digestion reaction temperature is 50 DEG C, the enzyme digestion reaction time is 3 hours, treat that enzyme digestion reaction terminates, enzymolysis solution is warming up to 80 DEG C of enzymes 20 minutes of going out, centrifugal after regulating enzymolysis solution pH to 4.6, centrifugal condition is: rotating speed 5000r/min, centrifugal 10min, obtain the first layer edible vegetable oil successively, second layer emulsion layer, third layer waste liquid and the 4th layer of crude protein, successively draw and obtain a shinyleaf yellowhorn oil, an emulsion layer and a crude protein liquid, an emulsion layer is separated, the freezing breakdown of emulsion that thaws again, one time breakdown of emulsion condition is: freezing temp-25 DEG C, freezing time 24 hours, thaw point 40 DEG C, thaw 4 hours, obtain an emulsion and it is centrifugal, centrifugal condition is: rotating speed 5000/min, centrifugal 10min, obtain the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, successively draw and obtain secondary shinyleaf yellowhorn oil and second emulsifying layer, be separated by second emulsifying layer, the freezing breakdown of emulsion that thaws again, described secondary breakdown of emulsion condition is: freezing temp-25 DEG C, freezing time 24 hours, thaw point 40 DEG C, thaws 4 hours, obtains secondary emulsion and it is centrifugal, centrifugal condition is: rotating speed 5000r/min, centrifugal 10min, obtains the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, draws upper strata edible vegetable oil and obtains three shinyleaf yellowhorn oils, and mix with a shinyleaf yellowhorn oil and secondary shinyleaf yellowhorn oil, obtained shinyleaf yellowhorn oil, a crude protein liquid is carried out an alkali carry, the condition that alkali is carried is: alkali carries solid-liquid ratio 1:5, alkali carries temperature 60 C, alkali carries pH8.5, and alkali carries time 2 h, obtains an alkali extract and it is centrifugal, centrifugal condition is: rotating speed 4000r/min, centrifugal 10min, obtains upper strata protein solution and lower sediment, is isolated and obtains a protein liquid and primary sedimentation, primary sedimentation is carried out secondary alkali carry, the condition that secondary alkali is carried is: alkali carries solid-liquid ratio 1:15, alkali carries temperature 60 C, alkali carries pH8.5, and alkali carries time 2 h, obtains secondary alkali extract and it is centrifugal, centrifugal condition is: rotating speed 4000r/min, centrifugal 10min, obtains upper strata protein solution and lower sediment, is isolated and obtains secondary protein liquid, by a protein liquid and the mixing of secondary protein liquid, add hydrochloric acid or lemon acid for adjusting pH to 4.6 to carry out acid and sink, the heavy temperature of acid is 40 DEG C, centrifugal after floss to appear, centrifugal condition is: rotating speed 4000r/min, and centrifugal 10min, is precipitated powder, lyophilize is carried out to precipitated powder, obtains Wood of Shinyleaf Yellowhorn albumen, shinyleaf yellowhorn oil yield 37.0%, extraction yield 65.9%, Wood of Shinyleaf Yellowhorn purity of protein (butt) 56.2%, extraction yield 44.7%.Wherein, Wood of Shinyleaf Yellowhorn benevolence oil length is 56.1%, and protein content is 28.9%.
Claims (9)
1. aqueous enzymatic method prepares a method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, it is characterized in that, comprises the steps:
Step one, slurries obtain: by Wood of Shinyleaf Yellowhorn benevolence raw material and water mixing, obtained slurries;
Step 2, enzymolysis: after regulating slurries pH, add polysaccharide composite enzyme enzymolysis, treat that enzyme digestion reaction terminates, enzymolysis solution is centrifugal, obtain the first layer edible vegetable oil, second layer emulsion layer, third layer waste liquid and the 4th layer of crude protein successively, successively draw and obtain shinyleaf yellowhorn oil, an emulsion layer and a crude protein liquid;
Step 3, a breakdown of emulsion: emulsion layer be separated, the freezing breakdown of emulsion that thaws again, obtain an emulsion and it is centrifugal, obtains the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, successively draw and obtain secondary shinyleaf yellowhorn oil and second emulsifying layer;
Step 4, secondary breakdown of emulsion: second emulsifying layer is separated, the freezing breakdown of emulsion that thaws again, obtain secondary emulsion and it is centrifugal, obtain the first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid successively, draw upper strata edible vegetable oil and obtain three shinyleaf yellowhorn oils, and mix with a shinyleaf yellowhorn oil and secondary shinyleaf yellowhorn oil, obtained shinyleaf yellowhorn oil;
Step 5, an alkali are carried: a crude protein liquid is carried out an alkali and carries, and obtain an alkali extract and it is centrifugal, obtain upper strata protein solution and lower sediment, are isolated and obtain a protein liquid and primary sedimentation;
Step 6, secondary alkali are carried: primary sedimentation is carried out secondary alkali and carries, and obtain secondary alkali extract and it is centrifugal, obtain upper strata protein solution and lower sediment, are isolated and obtain secondary protein liquid;
Step 7, acid are sunk: by a protein liquid and the mixing of secondary protein liquid, carry out acid heavy centrifugal, be precipitated powder, carry out lyophilize to precipitated powder, obtain Wood of Shinyleaf Yellowhorn albumen after regulating pH.
2. aqueous enzymatic method as claimed in claim 1 prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, it is characterized in that, Wood of Shinyleaf Yellowhorn benevolence stock preparation process in described step one is: decortication of being shelled by Wood of Shinyleaf Yellowhorn obtains pure Wood of Shinyleaf Yellowhorn benevolence, pure Wood of Shinyleaf Yellowhorn benevolence pulverized and sieved, obtaining granular size is 40 ~ 80 object Wood of Shinyleaf Yellowhorn benevolence raw materials.
3. aqueous enzymatic method as claimed in claim 2 prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, it is characterized in that, the slurries in described step one mix obtained by Wood of Shinyleaf Yellowhorn benevolence raw material and water being pressed solid-liquid ratio 1g:4 ~ 6mL.
4. aqueous enzymatic method as claimed in claim 3 prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, it is characterized in that, in slurries, hydrochloric acid is added or slurries pH is adjusted to 4 ~ 6 by citric acid in described step 2, add polysaccharide composite enzyme enzymolysis, enzyme digestion reaction temperature is 45 ~ 55 DEG C, and the enzyme digestion reaction time is 2 ~ 6 hours, treats that enzyme digestion reaction terminates, enzymolysis solution is warming up to 75 ~ 85 DEG C of enzymes 15 ~ 25 minutes of going out, centrifugal after regulating enzymolysis solution pH to 4 ~ 5.
5. aqueous enzymatic method as claimed in claim 4 prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, it is characterized in that, polysaccharide composite enzyme in described step 2 adopts cellulase+polygalacturonase, the consumption of described cellulase and polygalacturonase is 1:0.5 ~ 10, and the enzyme concentration of polysaccharide composite enzyme is 500 ~ 2000u/g of Wood of Shinyleaf Yellowhorn benevolence raw material dosage.
6. aqueous enzymatic method as claimed in claim 1 prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, and it is characterized in that, a described breakdown of emulsion and secondary breakdown of emulsion condition are: freezing temp-25 DEG C, freezing time 24 hours, and thaw point 40 DEG C thaws 4 hours.
7. aqueous enzymatic method as claimed in claim 1 prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, it is characterized in that, the condition that in described step 5, alkali is carried is: alkali carries solid-liquid ratio 1:5 ~ 10, alkali temperature raising degree 50 ~ 60 DEG C, alkali carries pH8 ~ 10, and alkali carries 1 ~ 3 hour time.
8. aqueous enzymatic method as claimed in claim 7 prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, it is characterized in that, the condition that in described step 6, secondary alkali is carried is: alkali carries solid-liquid ratio 1:10 ~ 15, alkali temperature raising degree 50-60 DEG C, alkali carries pH8-10, and alkali carries 0.5 ~ 2 hour time.
9. aqueous enzymatic method as claimed in claim 1 prepares the method for shinyleaf yellowhorn oil and Wood of Shinyleaf Yellowhorn albumen simultaneously, it is characterized in that, by a protein liquid and the mixing of secondary protein liquid in described step 7, add hydrochloric acid or lemon acid for adjusting pH to 4.6 to carry out acid and sink, the heavy temperature of acid is 40 DEG C, centrifugal after floss to appear.
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