CN103436353A - Shinyleaf yellowhorn oil extraction method - Google Patents
Shinyleaf yellowhorn oil extraction method Download PDFInfo
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- CN103436353A CN103436353A CN2013103995888A CN201310399588A CN103436353A CN 103436353 A CN103436353 A CN 103436353A CN 2013103995888 A CN2013103995888 A CN 2013103995888A CN 201310399588 A CN201310399588 A CN 201310399588A CN 103436353 A CN103436353 A CN 103436353A
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Abstract
The invention belongs to the field of food processing and relates to a shinyleaf yellowhorn oil extraction method. The extraction method comprises the following steps: picking shinyleaf yellowhorn nuts, crushing, carrying out enzyme deactivation, carrying out ultrasonic pretreatment, adding pectinase, cellulase and an accelerator, carrying out enzymolysis, and carrying out centrifugation, thereby obtaining shinyleaf yellowhorn oil, wherein the pectinase accelerator is (2,4,6-trihydroxyphenyl)-4-(4-hydroxyphenyl)pyrazole. A preparation method comprises the following steps: dissolving genistein into ethanol, then, subjecting oil-bath heating together with hydrazine hydrate, carrying out reflux reaction, and then, cooling to room temperature; after a product is cooled, adjusting the pH value to generate precipitates; re-crystallizing the precipitates, and drying so as to obtain products. The shinyleaf yellowhorn oil extraction method provided by the invention has the advantages that the defects are overcome, the process is simple, the types of equipment are few, the operation is easy, the oil yield is high, the quality of the oil is good, the process is simple and safe, the oil preparation period is short, the cost is low, and shinyleaf yellowhorn protein or shinyleaf yellowhorn protein peptide can be prepared simultaneously.
Description
Technical field
The invention belongs to the processing of farm products field, relate to a kind of extracting method of shinyleaf yellowhorn oil.
Background technology
Wood of Shinyleaf Yellowhorn belongs to the Sapindaceae Wood of Shinyleaf Yellowhorn and belongs to, and also is referred to as civil official fruit, xanthoceras sobifolia bunge, Wen Guanhua etc., is the woody seeds of the distinctive preciousness of China.It is not only a kind of nutritious, fruit of delicious flavour and famous woody oil tree species, but also is a kind of important good soil conservation seeds that can improve the ecological environment, afforest territory.
Wood of Shinyleaf Yellowhorn contains a large amount of greases, and the seed oil length is 30.4%, and kind benevolence is nutritious, and oil length is 55%～66%, and in its oil content, unsaturated fatty acid content is up to 94%, and wherein linolic acid accounts for 36.9%, and oleic acid accounts for 57.2%; And containing 19 seed amino acids of needed by human body, the various trace elements such as potassium, sodium, calcium, magnesium, iron, zinc and vitamin A, the multivitamins such as C, E.Shinyleaf yellowhorn oil is the very high food oils of a kind of nutritive value as can be seen here.At present, on world market Wood of Shinyleaf Yellowhorn oil price lattice 12 dollars/more than kg, future still will rise.
The main method of China's prepairng vegetable oil is traditional milling process and extraction at present.Traditional milling process technological operation is simple, strong adaptability, but the high temperature produced in expressing process more than 200 ℃ has caused significantly and has destroyed other important nutritive ingredients such as the unsaturated fatty acids in grease and vitamin Ks, also cause protein denaturation etc., its grouts can only be as animal-feed etc.; Relatively traditional milling process, extraction is a kind of comparatively advanced method of making oil, this method labour intensity is low, production efficiency is higher, but for the loose volatilization of can overflowing of the organic solvent of oil extraction, thereby large to human body and environment disadvantageous effect, under dissolvent residual, oil quality, the degradation problem makes its exploitation as edible oil and health-care oil be subject to certain restriction.Thus, in the urgent need to developing, new oil preparation process comes efficiently, shinyleaf yellowhorn oil high-quality, nutritive health-care is produced in industrialization.
Summary of the invention
The extracting method that the purpose of this invention is to provide a kind of shinyleaf yellowhorn oil, overcome above-mentioned defect, technique is simple, device category is few, easy to operate, oil yield is high, oil quality is good, technique is simple and safe, the liquefaction cycle is short, cost is low, and can prepare the extracting method of a kind of shinyleaf yellowhorn oil of Wood of Shinyleaf Yellowhorn albumen or Wood of Shinyleaf Yellowhorn protein peptide simultaneously.Its technical scheme is:
The extracting method of a kind of shinyleaf yellowhorn oil of the present invention, is characterized in that, selects Wood of Shinyleaf Yellowhorn benevolence, pulverize, and the enzyme that goes out, ultrasonic pretreatment, add polygalacturonase, cellulase and promotor, enzymolysis, the centrifugal shinyleaf yellowhorn oil that obtains.
Wherein, enzyme promotor is (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles, the preparation method is:
(1) after genistein is dissolved in to ethanol, with hydrazine hydrate, carry out the oil bath heating, be cooled to room temperature after back flow reaction;
(2) cooled product, regulator solution pH, produce precipitation;
(3) will precipitate after recrystallization, drying to obtain product.
The enzyme of promotor effect is mainly take polygalacturonic acid as main polygalacturonase and cellulase.
The 0.5%-2% that the addition of polygalacturonase is Wood of Shinyleaf Yellowhorn benevolence quality; The 0.5%-2% cellulase that the addition of cellulase is Wood of Shinyleaf Yellowhorn benevolence quality; The addition that the quality that adds the 2%-4% of all enzyme quality sums is enzyme promotor.
The pH of enzymolysis is 3.8-5.8, and temperature is under 48-50 ℃ of condition; Enzymolysis time is 4-6 hour.
Enzyme promotor is (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles; Structural formula is.
The amount of substance of genistein: ethanol volume: the hydrazine hydrate volume is 1:90-110:12-18.
The temperature refluxed is 75-90 ℃; Return time is 1-3h.
Use the salt acid for adjusting pH value, the pH value is 4-5.
The volume of recrystallization is that water and ethanol volume ratio are the mixed solvent that 8.5-9.5:1 forms.
Dry temperature is 70-90 ℃.
Wood of Shinyleaf Yellowhorn Renhe water is 1 g by solid-liquid ratio: 6 ~ 8 mL mix.
Ultrasonic frequency is 28kHz or 52kHz, and power is 150-300W, and temperature is 45-50 ℃, and the time is 10-25min.
Compared with prior art, the present invention has following beneficial effect:
1, the extracting method of a kind of shinyleaf yellowhorn oil of the present invention, adopt enzyme promotor to improve polygalacturonase and cellulase activity, pectin and Mierocrystalline cellulose execution have been improved, accelerated the stripping of shinyleaf yellowhorn oil, improved the yield of shinyleaf yellowhorn oil, and the shinyleaf yellowhorn oil quality is high, retain the nutritive ingredient of shinyleaf yellowhorn oil and the peculiar flavour of Wood of Shinyleaf Yellowhorn.
2, reduce the usage quantity of polygalacturonase and cellulase, reduced production process, reduced production cost, and improved the quality of product, extended the quality guaranteed period of product.Use enzyme promotor to have important practical significance to comprehensive utilization, the raising Wood of Shinyleaf Yellowhorn economic value added of Wood of Shinyleaf Yellowhorn.
Embodiment
Below in conjunction with embodiment, the present invention is described further.
Embodiment 1
A kind of extracting method of shinyleaf yellowhorn oil, preparation process is as follows:
To be crushed to 40 purpose Wood of Shinyleaf Yellowhorn Renhe water is 1 g by solid-liquid ratio: after 6 mL mix, be placed in the water-bath that temperature is 90 ℃, magnetic agitation is processed 5 min and is gone out cooling after enzyme.45 ℃ of temperature, under frequency 28 kHz, pre-treatment 15 min under ultrasonic 250W; The pH of the slurries of milled is adjusted to 5.8, adds the polygalacturonase of Wood of Shinyleaf Yellowhorn benevolence quality 0.5% and 2% cellulase, add 2% enzyme promotor of polygalacturonase and cellulase quality sum.In 50 ℃ of water-baths, enzymolysis is 6 hours; Enzymolysis solution is centrifugal, 15000 rev/mins, centrifugal 20 minutes; After centrifugal, draw upper strata edible vegetable oil, obtain shinyleaf yellowhorn oil;
Wherein, the preparation method of enzyme promotor is:
(1) 0.1 mmol genistein is dissolved in 10 mL ethanol, and the hydrazine hydrate of 1.5 mL80% is placed in the round-bottomed flask with reflux, the oil bath heating, at 85 ℃ of lower back flow reaction 2 h, be cooled to room temperature;
(2) cooled product, adding hydrochloric acid conditioning solution pH is 4, produces precipitation;
(3) water and ethanol volume ratio are that the mixed solvent that 9:1 forms carries out recrystallization, obtain product after 70 ℃ of dryings, are (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles.
With the shinyleaf yellowhorn oil that does not use enzyme (extraction yield of oil is 21.4%), compare, the extraction yield of oil is 47.3%, has improved 221%; With using polygalacturonase promotor (extraction yield of oil is 41.5%), do not compare, oily yield has improved 14%.
Embodiment 2
A kind of extracting method of shinyleaf yellowhorn oil, preparation process is as follows:
To be crushed to 40 purpose Wood of Shinyleaf Yellowhorn Renhe water is 1 g by solid-liquid ratio: after 8 mL mix, be placed in the water-bath that temperature is 90 ℃, magnetic agitation is processed 5 min and is gone out cooling after enzyme.45 ℃ of temperature, under frequency 52 kHz, pre-treatment 25 min under ultrasonic 300W; The pH of the slurries of milled is adjusted to 5.8, adds the polygalacturonase of Wood of Shinyleaf Yellowhorn benevolence quality 2% and 0.5% cellulase, add 4% enzyme promotor of polygalacturonase and cellulase quality sum.In 48 ℃ of water-baths, enzymolysis is 4 hours; Enzymolysis solution is centrifugal, 18000 rev/mins, centrifugal 30 minutes; After centrifugal, draw upper strata edible vegetable oil, obtain shinyleaf yellowhorn oil;
Wherein, the preparation method of enzyme promotor is:
(1) 0.1 mmol genistein is dissolved in 10 mL ethanol, and the hydrazine hydrate of 1.5 mL80% is placed in the round-bottomed flask with reflux, the oil bath heating, at 85 ℃ of lower back flow reaction 2 h, be cooled to room temperature;
(2) cooled product, adding hydrochloric acid conditioning solution pH is 4.5, produces precipitation;
(3) water and ethanol volume ratio are that the mixed solvent that 9:1 forms carries out recrystallization, obtain product after 90 ℃ of dryings, are (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles.
With the shinyleaf yellowhorn oil that does not use enzyme (extraction yield of oil is 22.8%), compare, the extraction yield of oil is 61.3%, has improved 269%; With using polygalacturonase promotor (extraction yield of oil is 51.1%), do not compare, oily extraction yield has improved 20%.
Claims (12)
1. the extracting method of a shinyleaf yellowhorn oil, is characterized in that, selects Wood of Shinyleaf Yellowhorn benevolence, pulverize, and the enzyme that goes out, ultrasonic pretreatment, add polygalacturonase, cellulase and promotor, enzymolysis, the centrifugal shinyleaf yellowhorn oil that obtains;
Wherein, enzyme promotor is (2,4,6-trihydroxy-phenyl)-4-(4-hydroxy phenyl) pyrazoles, the preparation method is:
After genistein is dissolved in to ethanol, with hydrazine hydrate, carry out the oil bath heating, be cooled to room temperature after back flow reaction;
After cooled product, regulator solution pH, produce precipitation;
Obtain product after precipitating recrystallization, drying.
2. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, enzyme is polygalacturonase and cellulase.
3. polygalacturonase is that polygalacturonic acid is main polygalacturonase.
4. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1 and 2, is characterized in that, the 0.5%-2% that the addition of polygalacturonase is Wood of Shinyleaf Yellowhorn benevolence quality; The 0.5%-2% cellulase that the addition of cellulase is Wood of Shinyleaf Yellowhorn benevolence quality; The addition that the quality that adds the 2%-4% of all enzyme quality sums is enzyme promotor.
5. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, the pH of enzymolysis is 3.8-5.8, and temperature is under 48-50 ℃ of condition; Enzymolysis time is 4-6 hour; The temperature 95-100 ℃ of enzyme goes out.
6. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, the amount of substance of genistein: the ethanol volume: the hydrazine hydrate volume is 1:90-110:12-18.
7. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, the temperature of backflow is 75-90 ℃; Return time is 1-3h.
8. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, the volume of recrystallization is that water and ethanol volume ratio are the mixed solvent that 8.5-9.5:1 forms.
9. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, dry temperature is 70-90 ℃.
10. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, ultrasonic frequency is 28kHz or 52kHz, and power is 150-300W, and temperature is 45-50 ℃, and the time is 10-25min.
11. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, Wood of Shinyleaf Yellowhorn Renhe water is 1 g by solid-liquid ratio: 6 ~ 8 mL mix.
12. the extracting method of a kind of shinyleaf yellowhorn oil according to claim 1, is characterized in that, in step (2), uses the salt acid for adjusting pH value, the pH value is 4-5.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212631A (en) * | 2014-09-10 | 2014-12-17 | 甘肃省轻工研究院 | Method of extracting shiny-leaf yellowhorn oil from shiny-leaved yellowhorn seeds |
| CN104788535A (en) * | 2015-04-27 | 2015-07-22 | 武汉轻工大学 | Method for simultaneously preparing shinyleaf yellowhorn oil and shinyleaf yellowhorn protein with aqueous enzymatic method |
| CN110862862A (en) * | 2019-11-28 | 2020-03-06 | 内蒙古农业大学 | Method for simultaneously preparing xanthoceras sorbifolia Bunge oil and xanthoceras sorbifolia Bunge protein by aqueous enzymatic method |
| CN113046172A (en) * | 2021-03-22 | 2021-06-29 | 安徽理工大学 | Extraction method of luffa seed oil |
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| CN1416843A (en) * | 2001-11-03 | 2003-05-14 | 福州济安堂菌草酸酶技术开发有限公司 | Enzyme prepn method of preparing Chinese medicine |
| CN101695321A (en) * | 2009-10-23 | 2010-04-21 | 山东理工大学 | Method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction |
| CN103060078A (en) * | 2011-10-20 | 2013-04-24 | 中国科学院兰州化学物理研究所 | Method for aqueous enzymatic extraction of gardenia oil |
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2013
- 2013-09-06 CN CN201310399588.8A patent/CN103436353B/en not_active Expired - Fee Related
Patent Citations (3)
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| CN1416843A (en) * | 2001-11-03 | 2003-05-14 | 福州济安堂菌草酸酶技术开发有限公司 | Enzyme prepn method of preparing Chinese medicine |
| CN101695321A (en) * | 2009-10-23 | 2010-04-21 | 山东理工大学 | Method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212631A (en) * | 2014-09-10 | 2014-12-17 | 甘肃省轻工研究院 | Method of extracting shiny-leaf yellowhorn oil from shiny-leaved yellowhorn seeds |
| CN104788535A (en) * | 2015-04-27 | 2015-07-22 | 武汉轻工大学 | Method for simultaneously preparing shinyleaf yellowhorn oil and shinyleaf yellowhorn protein with aqueous enzymatic method |
| CN104788535B (en) * | 2015-04-27 | 2018-02-02 | 武汉轻工大学 | The method that aqueous enzymatic method prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously |
| CN110862862A (en) * | 2019-11-28 | 2020-03-06 | 内蒙古农业大学 | Method for simultaneously preparing xanthoceras sorbifolia Bunge oil and xanthoceras sorbifolia Bunge protein by aqueous enzymatic method |
| CN113046172A (en) * | 2021-03-22 | 2021-06-29 | 安徽理工大学 | Extraction method of luffa seed oil |
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