CN101695321A - Method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction - Google Patents
Method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction Download PDFInfo
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- CN101695321A CN101695321A CN200910019388A CN200910019388A CN101695321A CN 101695321 A CN101695321 A CN 101695321A CN 200910019388 A CN200910019388 A CN 200910019388A CN 200910019388 A CN200910019388 A CN 200910019388A CN 101695321 A CN101695321 A CN 101695321A
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Abstract
The invention provides a method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, comprising the following steps: (1) pouring shinyleaf yellowhorn kernel and water into a colloid mill for crushing according to a ratio of material to liquid (g/mL) of 1:4-8, crushing the shinyleaf yellowhorn kernel into particles of 70-160 mu and collecting slurry; (2) adjusting the pH of the milled slurry to 3.8-5.8, adding cellulose accounting for 0.5-2.5 percent of the weight of the shinyleaf yellowhorn kernel and carrying out enzymolysis for 3-8 hours in a water bath at 50-60 DEG C; (3) centrifuging the enzymolysis liquid at the speed of 4000-6000 rpm for 20-30 minutes, and sucking clear oil at the upper layer after the centrifugation; (4) adjusting the pH of the enzymolysis liquid at the lower layer to 6.8-7.3, adding alkaline protease accounting for 1.8-2.5 percent of the weight of the shinyleaf yellowhorn kernel, and reacting in a water bath at 50-60 DEG C for 2.5-3.5 hours; and (5) centrifuging the enzymolysis liquid at the speed of 4500-6000 rpm for 30-35 minutes, sucking yellow clear oil at the upper layer, and mixing the yellow clear oil with the clear oil sucked at the first time, wherein the yellow clear oil and the clear oil sucked at the first time can be independently used. The invention has the advantages of high oil yield, good oil quality, simple and safe process, short oil making period and low cost, and hinyleaf yellowhorn protein and hinyleaf yellowhorn protein peptide can be simultaneously prepared.
Description
Technical field
The invention provides a kind of method of extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, belong to technical field of agricultural product process.
Background technology
Shiny-leaved yellowhorn belongs to the Sapindaceae shiny-leaved yellowhorn and belongs to, and also is referred to as civil official fruit, xanthoceras sobifolia bunge, Wen Guanhua etc., is the woody seeds of the distinctive preciousness of China.It is not only a kind of nutritious, fruit of delicious flavour and famous woody oil tree species, but also is a kind of important good water and soil conservation seeds that can improve the ecological environment, afforest the territory.
Shiny-leaved yellowhorn contains a large amount of greases, and the seed oil content is 30.4%, and kind benevolence is nutritious, and oil content is 55%～66%, and unsaturated fatty acid content is up to 94% in its oil content, and wherein linoleic acid accounts for 36.9%, and oleic acid accounts for 57.2%; And contain 19 seed amino acids of needed by human body, various trace elements such as potassium, sodium, calcium, magnesium, iron, zinc and vitamin A, multivitamins such as C, E.This shows that shinyleaf yellowhorn oil is the very high edible oil and fat of a kind of nutritive value.At present, on the international market shiny-leaved yellowhorn oil price lattice 12 dollars/more than the kg, estimate that still will rise future.
The main method that present China produces vegetable oil is traditional milling process and extraction.Traditional milling process technological operation is simple, adaptability is strong, but the high temperature that produces in the expressing process more than 200 ℃ has caused destruction significantly to other important nutritional labelings such as unrighted acid in the grease and vitamin Ks, also cause protein denaturation etc., its grouts can only be as animal feed etc.; Traditional relatively milling process, extraction is a kind of comparatively advanced method for producing oil, this method labour intensity is low, production efficiency is higher, the volatilization of loosing but the organic solvent that is used for the grease leaching can overflow, thereby big to human body and environment adverse effect, the degradation problem makes its exploitation as edible oil and health care oil be subjected to certain restriction under dissolvent residual, the oil quality.Thus, pressing for the new oil preparation process of exploitation comes efficient, industrialization to produce shinyleaf yellowhorn oil high-quality, nourishing healthy.
Summary of the invention
The purpose of this invention is to provide a kind ofly can overcome above-mentioned defective, technology is simple, device category is few, easy to operate, oil extracting rate is high, oil quality good, technology is simple and safe, the system oil cycle is short, cost is low, and can prepare the method for the extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction of shiny-leaved yellowhorn albumen or shiny-leaved yellowhorn protein peptides simultaneously.Its technical scheme is:
A kind of method of extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction is characterized in that adopting following steps:
1) be 1g with shiny-leaved yellowhorn Renhe water by solid-liquid ratio: 4～8mL pours in the colloid mill and pulverizes, and pulverizes shiny-leaved yellowhorn benevolence to the 70-160 order, collects slurries;
2) pH that will grind good slurries is adjusted to 3.8-5.8, adds the enzyme of shiny-leaved yellowhorn benevolence quality 0.5-2.5%, in 50-60 ℃ of water-bath enzymolysis 3-8 hour; 3) enzymolysis liquid is centrifugal, 4000-6000 rev/min, centrifugal 20-30 minute; Behind centrifugal the finishing, draw upper strata edible vegetable oil;
4) the enzymolysis liquid pH that will be positioned at lower floor is adjusted to 6.8-7.3, adds the alkali protease of shiny-leaved yellowhorn benevolence quality 1.8～2.5%, and reaction is 2.5-3.5 hour in 50-60 ℃ of water-bath;
5) with enzymolysis liquid at 4500-6000 rev/min of centrifugal 30-35 minute, the yellow edible vegetable oil in sucking-off upper strata can merge with the edible vegetable oil of the sucking-off first time, also can use separately.
The method of described extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, step 1) Chinese hat kernel is the shiny-leaved yellowhorn benevolence of shelling and peeling.
The method of described extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, step 1) Chinese hat kernel carries out steeping more than 0.5 hour in advance earlier before the colloid mill.
The method of described extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, step 2) acid of used accent pH is one or both in hydrochloric acid or the citric acid in, and joins enzyme liquid again after modulating pH value of solution.
The method of described extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, step 2) enzyme adopts cellulase or cellulase+papain or cellulase+acid protease in, if adopt cellulase+papain, the papain consumption is the 0.05-2% of shiny-leaved yellowhorn benevolence quality, if adopt cellulase+acid protease, the acidic protein enzyme dosage is the 0.1-0.5% of shiny-leaved yellowhorn benevolence quality.
The method of described extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, the alkali of transferring pH in the step 4) is one or both in NaOH or the potassium hydroxide, and joins in the enzyme liquid after modulating pH value of solution again.
The method of described extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, lower floor's enzymolysis liquid of enzymolysis liquid is concentrated through reducing pressure in the step 5), can obtain activated protein peptide concentrate, then can obtain activated protein peptide powder through freeze drying.
The present invention compared with prior art, the technology that is adopted is simple, reagent and equipment, instrument kind are few, the shinyleaf yellowhorn oil quality better, the recovery rate height, improve about 10% than the additive method recovery rate, but also can obtain shiny-leaved yellowhorn activated protein peptide concentrate, perhaps shiny-leaved yellowhorn activated protein peptide powder or shiny-leaved yellowhorn albumen.
Preferred forms
Embodiment 1:
Is to pour in colloid mill at 1: 5 to pulverize with the shiny-leaved yellowhorn benevolence 800g and the water that are steeping the decortication of shelling of 1h by solid-liquid ratio (g/mL), and grinding and sieving reaches the 70-160 order until shiny-leaved yellowhorn benevolence, collects slurries.It is 5.8 that the slurries of collecting are transferred pH with hydrochloric acid, adds the cellulase of shiny-leaved yellowhorn benevolence quality 2.5%, and enzymolysis is 8 hours in 50 ℃ of water-baths; Enzymolysis liquid is centrifugal, 4000 rev/mins, centrifugal 30 minutes, behind centrifugal the finishing, draw upper strata edible vegetable oil; The enzymolysis liquid pH that will be positioned at lower floor is adjusted to 6.8, adds the alkali protease of shiny-leaved yellowhorn benevolence quality 2.5%, and reaction is 2.5 hours in 60 ℃ of water-baths; With enzymolysis liquid 4500 rev/mins centrifugal 30 minutes, the yellow edible vegetable oil in sucking-off upper strata merges with the edible vegetable oil of the sucking-off first time.Shiny-leaved yellowhorn benevolence oil yield is 51.1%, and recovery rate is 89.3%.Annotate: shiny-leaved yellowhorn benevolence oil content is 57.2%.
Embodiment 2:
Is to pour in colloid mill at 1: 8 to pulverize with the shiny-leaved yellowhorn benevolence 700g and the water that are steeping the decortication of shelling of 1h by solid-liquid ratio (g/mL), and grinding and sieving reaches the 70-160 order until shiny-leaved yellowhorn benevolence, collects slurries.It is 3.8 that the slurries of collecting are transferred pH with citric acid, adds the cellulase of shiny-leaved yellowhorn benevolence quality 0.5% and 0.1% papain of shiny-leaved yellowhorn benevolence quality, and enzymolysis is 5 hours in 60 ℃ of water-baths; Enzymolysis liquid is centrifugal, 6000 rev/mins, centrifugal 30 minutes, behind centrifugal the finishing, draw upper strata edible vegetable oil.Shiny-leaved yellowhorn benevolence oil yield is 42.3%, and recovery rate is 84.0%.Annotate: shiny-leaved yellowhorn benevolence oil content is 50.4%.
Embodiment 3:
Is to pour in colloid mill at 1: 8 to pulverize with the shiny-leaved yellowhorn benevolence 700g and the water that are steeping the decortication of shelling of 1h by solid-liquid ratio (g/mL), and grinding and sieving reaches the 70-160 order until shiny-leaved yellowhorn benevolence, collects slurries.Is 3.8 with what grind good slurries with citric acid accent pH, adds the cellulase of shiny-leaved yellowhorn benevolence quality 0.5% and 0.2% acid protease of shiny-leaved yellowhorn benevolence quality, and enzymolysis is 5 hours in 60 ℃ of water-baths; Enzymolysis liquid is centrifugal, 6000 rev/mins, centrifugal 30 minutes, behind centrifugal the finishing, draw upper strata edible vegetable oil.Shiny-leaved yellowhorn benevolence oil yield is 41.8%, and recovery rate is 83.3%.Annotate: shiny-leaved yellowhorn benevolence oil content is 50.2%.
Claims (7)
1. the method for an extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction is characterized in that adopting following steps:
1) be 1g with shiny-leaved yellowhorn Renhe water by solid-liquid ratio: 4～8mL pours in the colloid mill and pulverizes, and pulverizes shiny-leaved yellowhorn benevolence to the 70-160 order, collects slurries;
2) pH that will grind good slurries is adjusted to 3.8-5.8, adds the enzyme of shiny-leaved yellowhorn benevolence quality 0.5-2.5%, in 50-60 ℃ of water-bath enzymolysis 3-8 hour;
3) enzymolysis liquid is centrifugal, 4000-6000 rev/min, centrifugal 20-30 minute; Behind centrifugal the finishing, draw upper strata edible vegetable oil;
4) the enzymolysis liquid pH that will be positioned at lower floor is adjusted to 6.8-7.3, adds the alkali protease of shiny-leaved yellowhorn benevolence quality 1.8～2.5%, and reaction is 2.5-3.5 hour in 50-60 ℃ of water-bath;
5) with enzymolysis liquid at 4500-6000 rev/min of centrifugal 30-35 minute, the yellow edible vegetable oil in sucking-off upper strata can merge with the edible vegetable oil of the sucking-off first time, also can use separately.
2. the method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction as claimed in claim 1 is characterized in that: step 1) Chinese hat kernel is the shiny-leaved yellowhorn benevolence of decortication of shelling.
3. the method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction as claimed in claim 1 is characterized in that: step 1) Chinese hat kernel carries out steeping more than 0.5 hour in advance earlier before the colloid mill.
4. the method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction as claimed in claim 1 is characterized in that: step 2) in the acid of used accent pH be in hydrochloric acid or the citric acid one or both, and join enzyme liquid again after modulating pH value of solution.
5. the method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction as claimed in claim 1, it is characterized in that: step 2) middle enzyme employing cellulase or cellulase+papain or cellulase+acid protease, if adopt cellulase+papain, the papain consumption is the 0.05-2% of shiny-leaved yellowhorn benevolence quality, if adopt cellulase+acid protease, the acidic protein enzyme dosage is the 0.1-0.5% of shiny-leaved yellowhorn benevolence quality.
6. the method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction as claimed in claim 1 is characterized in that: the alkali of transferring pH in the step 4) is one or both in NaOH or the potassium hydroxide, and joins in the enzyme liquid after modulating pH value of solution again.
7. the method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction as claimed in claim 1, it is characterized in that: lower floor's enzymolysis liquid of enzymolysis liquid is concentrated through reducing pressure in the step 5), can obtain activated protein peptide concentrate, then can obtain activated protein peptide powder through freeze drying.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948897A (en) * | 2010-09-11 | 2011-01-19 | 山西金绿禾燕麦研究所 | Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn |
| CN102732370A (en) * | 2012-06-25 | 2012-10-17 | 东北农业大学 | Pilot plant test method for extracting soybean oil by using aqueous enzymatic method |
| CN102823803A (en) * | 2012-09-17 | 2012-12-19 | 晋城市伊健食品有限公司 | Decompression and lipid-lowering grain food of shiny-leaved yellowhorn nuts and preparation method thereof |
| CN102925282A (en) * | 2012-10-24 | 2013-02-13 | 师海全 | Method for extracting edible oil by squeezing ripe seeds of xanthoce sorbifolia bunge |
| CN103205309A (en) * | 2013-03-22 | 2013-07-17 | 西北师范大学 | Method for preparing sorbifolia seed kernel oil by enzyme-coupled salt method |
| CN103436350A (en) * | 2013-07-20 | 2013-12-11 | 烟台大学 | Aqueous enzymatic method for extracting rapeseed oil and recovering protein |
| CN103436353A (en) * | 2013-09-06 | 2013-12-11 | 山东理工大学 | Shinyleaf yellowhorn oil extraction method |
| CN103756775A (en) * | 2014-01-15 | 2014-04-30 | 盐城师范学院 | Method for extracting suaeda salsa seed oil with enzymolysis method |
| CN104788535A (en) * | 2015-04-27 | 2015-07-22 | 武汉轻工大学 | Method for simultaneously preparing shinyleaf yellowhorn oil and shinyleaf yellowhorn protein with aqueous enzymatic method |
| CN105238544A (en) * | 2015-11-12 | 2016-01-13 | 东北农业大学 | Making method of xanthoceras sorblfolia bunge seed oil |
| CN110862862A (en) * | 2019-11-28 | 2020-03-06 | 内蒙古农业大学 | Method for simultaneously preparing xanthoceras sorbifolia Bunge oil and xanthoceras sorbifolia Bunge protein by aqueous enzymatic method |
| CN115029405A (en) * | 2022-04-04 | 2022-09-09 | 国药肽谷有限公司 | Method for extracting xanthoceras sorbifolia bunge peptide |
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2009
- 2009-10-23 CN CN200910019388A patent/CN101695321A/en active Pending
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948897B (en) * | 2010-09-11 | 2013-02-13 | 山西金绿禾燕麦研究所 | Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn |
| CN101948897A (en) * | 2010-09-11 | 2011-01-19 | 山西金绿禾燕麦研究所 | Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn |
| CN102732370A (en) * | 2012-06-25 | 2012-10-17 | 东北农业大学 | Pilot plant test method for extracting soybean oil by using aqueous enzymatic method |
| CN102732370B (en) * | 2012-06-25 | 2013-09-04 | 东北农业大学 | Pilot test method for extracting soybean oil by using aqueous enzymatic method |
| CN102823803A (en) * | 2012-09-17 | 2012-12-19 | 晋城市伊健食品有限公司 | Decompression and lipid-lowering grain food of shiny-leaved yellowhorn nuts and preparation method thereof |
| CN102925282B (en) * | 2012-10-24 | 2014-03-12 | 师海全 | Method for extracting edible oil by squeezing ripe seeds of xanthoce sorbifolia bunge |
| CN102925282A (en) * | 2012-10-24 | 2013-02-13 | 师海全 | Method for extracting edible oil by squeezing ripe seeds of xanthoce sorbifolia bunge |
| CN103205309A (en) * | 2013-03-22 | 2013-07-17 | 西北师范大学 | Method for preparing sorbifolia seed kernel oil by enzyme-coupled salt method |
| CN103436350A (en) * | 2013-07-20 | 2013-12-11 | 烟台大学 | Aqueous enzymatic method for extracting rapeseed oil and recovering protein |
| CN103436353A (en) * | 2013-09-06 | 2013-12-11 | 山东理工大学 | Shinyleaf yellowhorn oil extraction method |
| CN103436353B (en) * | 2013-09-06 | 2014-09-24 | 山东理工大学 | Shinyleaf yellowhorn oil extraction method |
| CN103756775A (en) * | 2014-01-15 | 2014-04-30 | 盐城师范学院 | Method for extracting suaeda salsa seed oil with enzymolysis method |
| CN104788535A (en) * | 2015-04-27 | 2015-07-22 | 武汉轻工大学 | Method for simultaneously preparing shinyleaf yellowhorn oil and shinyleaf yellowhorn protein with aqueous enzymatic method |
| CN104788535B (en) * | 2015-04-27 | 2018-02-02 | 武汉轻工大学 | The method that aqueous enzymatic method prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously |
| CN105238544A (en) * | 2015-11-12 | 2016-01-13 | 东北农业大学 | Making method of xanthoceras sorblfolia bunge seed oil |
| CN105238544B (en) * | 2015-11-12 | 2021-05-14 | 东北农业大学 | Preparation method of xanthoceras sorbifolia bunge oil |
| CN110862862A (en) * | 2019-11-28 | 2020-03-06 | 内蒙古农业大学 | Method for simultaneously preparing xanthoceras sorbifolia Bunge oil and xanthoceras sorbifolia Bunge protein by aqueous enzymatic method |
| CN115029405A (en) * | 2022-04-04 | 2022-09-09 | 国药肽谷有限公司 | Method for extracting xanthoceras sorbifolia bunge peptide |
| CN115029405B (en) * | 2022-04-04 | 2023-08-22 | 国药肽谷有限公司 | Method for extracting shinyleaf yellowhorn peptide |
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Open date: 20100421 |