CN105520156A - Preparation method of grape seed soluble dietary fiber - Google Patents
Preparation method of grape seed soluble dietary fiber Download PDFInfo
- Publication number
- CN105520156A CN105520156A CN201510878888.3A CN201510878888A CN105520156A CN 105520156 A CN105520156 A CN 105520156A CN 201510878888 A CN201510878888 A CN 201510878888A CN 105520156 A CN105520156 A CN 105520156A
- Authority
- CN
- China
- Prior art keywords
- grape
- grape pip
- dietary fiber
- soluble dietary
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The present invention discloses a preparation method of grape seed soluble dietary fiber, comprising the following steps: (1) grape seed defatting treatment: petroleum ether is used as an extract agent to conduct defatting treatment of grape seeds, and the defatted grape seeds are dried and crushed; and (2) grape seed soluble dietary fiber enzymatic preparation method: an appropriate amount of water is added into the defatted grape seed powder, the mixture is sheared and emulsified, the emulsified mixture is treated using cellulose enzyme and alkaline protease, the treated mixture is centrifuged, filtrated, and precipitated using ethanol, and the obtained precipitate is dried to obtain a soluble dietary fiber. The yield of the grape seed extract soluble dietary fiber is 90%, the comprehensive utilization value of the grape seeds is improved, grape seed resource waste is comprehensive utilized, and the extraction cost is low and cheap.
Description
Technical field
The invention belongs to food technology of preparing neck, particularly relating to is a kind of preparation method of grape pip soluble dietary fiber.
Background technology
Grape pip is the leftover bits and pieces of grape wine factory, is separated products therefrom after Grape Skin, grape pedicel after drying.Grape pip contains effect that abundant several amino acids, carbohydrate, vitamin and mineral matter etc. have health care, beauty treatment.
At present, the research on utilization of grape pip is mainly concentrated on to the extraction and application of grape-kernel oil, OPC and polyphenol, about exploitation report coarse-fibred in grape pip seldom.
Different according to dissolubility, dietary fiber is divided into insoluble diedairy fiber (IDF, insolubledietaryfiber) and soluble dietary fiber (SDF, solubledietaryfiber).SDF can reduce the content of LDL-C in blood plasma, promotes beneficial flora growth and breeding in enteron aisle, can be used as the functional food of the diseases such as adjuvant therapy of cardiovascular.In addition, dietary fiber also can add in a lot of food as a kind of functional food ingredient, to improve nutritive value of food, thus promotes the development of functional food.The present invention for raw material, optimizes the method that enzyme process prepares soluble dietary fiber with the abundant grape pip of crude fiber content.
Summary of the invention
The object of the invention is a kind of preparation method of grape pip soluble dietary fiber.
The preparation method of soluble dietary fiber provided by the invention, comprises the steps:
1) preparation of defatted grape seed powder:
A) be 10KPa-20KPa by the grape skin after wine brewing in vacuum, temperature is 40 DEG C--dry under the cryogenic vacuum condition of 80 DEG C, drying time is 3-72 hour, through screening, grape pip is separated with Grape Skin, grape pedicel after drying, and make dried grape pip moisture content≤10%, clump count≤1000;
B) by grease in dried grape pip cold press removing grape pip, become the grape pip dregs of rice, in cold pressing process, make the temperature of grape pip be no more than 85 DEG C;
C) by the grape pip dregs of rice through pulverizer coarse crushing, be crushed to≤grape pip the dregs of rice particle of 1cm;
D) grape pip dregs of rice particle is worn into grape pip fine powder through flour mill;
E) grape pip fine powder dressing sieve machine is filtered out 80-30 object fine powder, be defatted grape seed powder;
2) preparation of soluble dietary fiber: take defatted grape seed powder, adds 20-30 times of weighing body ponding, emulsification pretreatment; Adjust pH4.5, add 2.0% cellulase, 40-50 DEG C, enzymolysis 8-10h; Be warming up to 50-60 DEG C, adjust pH to 8, then add the alkali protease enzymolysis 1-3h of 0.4%, go out enzyme; The centrifugal 10-20min of 6000-10000r/min, collects clear liquid, and filter, with 4 times of volume 98% alcohol settling 1h, standing separation, dry sediment obtains soluble dietary fiber.
Described step 2) in, in described emulsification pretreatment step, the rotating speed of emulsification pretreatment is 7000r, and the time of emulsification pretreatment is 30min.
Described step 2) in, the enzyme activity of cellulase described in enzymolysis step is 30,000 U/g, and the enzyme activity of described alkali protease is 200,000 U/g.
Described step 2) in, in drying steps, drying mode is at least one in forced air drying, pneumatic conveying drying, spraying dry, vacuum drying and freeze drying.
This step should control moisture in dried product exhibited below 10%.
Beneficial effect of the present invention is as follows:
1, this method is compared with traditional fractionation, and extraction conditions is gentle, and dietary fiber loss is few, and color and luster is good, and the waste liquid of generation is less;
2, this method is similar to traditional enzymatic isolation method, but the enzyme liquid consumed is less, and product purity is higher, and be that raw material extracts soluble dietary fiber with grape pip, gained soluble dietary fiber recovery rate is more than 95%, and purity is more than 80%;
3, emulsification pretreatment auxiliary enzymes solution only needs the devices such as emulsification pretreatment equipment, fermentation tank, without the need to increasing new equipment, is easy to industrialization.
4, improve the comprehensive utilization value of grape pip, to grape pip resource discarded object comprehensive development and utilization, extract low cost, non-environmental-pollution.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Described method is conventional method if no special instructions.
Embodiment 1
(1) preparation of defatted grape seed powder:
A) be 15KPa by the grape skin after wine brewing in vacuum, temperature is dry under the cryogenic vacuum condition of 60 DEG C, and drying time is 24 hours, is separated by grape pip after drying through screening with Grape Skin, grape pedicel, dried grape pip moisture content≤10%, clump count≤1000;
B) by grease in dried grape pip cold press removing grape pip, become the grape pip dregs of rice, in cold pressing process, make the temperature of grape pip be no more than 85 DEG C;
C) by the grape pip dregs of rice through pulverizer coarse crushing, be crushed to≤grape pip the dregs of rice particle of 1cm;
D) grape pip dregs of rice particle is worn into grape pip fine powder through flour mill;
E) grape pip fine powder dressing sieve machine is filtered out 80-30 object fine powder, be defatted grape seed powder;
(2) get 5.00g defatted grape seed powder, add pure water by solid-liquid ratio 1g: 20mL, rotating speed is 7000r emulsification pretreatment is 30min; Regulate pH4.5 with the hydrochloric acid solution of 1mol/L, add 0.1g cellulase (enzyme activity is 30,000 U/g)
40 DEG C, enzymolysis 10h; Be warming up to 50 DEG C, adjust pH to 8, then add 0.02g alkali protease (enzyme activity is 200,000 U/g) enzymolysis 3h, go out enzyme; The centrifugal 20min of 6000r/min, collects clear liquid, filters, and with 4 times of volume 98% alcohol settling 1h, standing separation, drying precipitatedly obtains soluble dietary fiber 0.35g.
After testing: the soluble dietary fiber purity 81.5% obtained.
Embodiment 2
In Example 1, the defatted grape seed powder 10.0g of step (1), adds pure water by solid-liquid ratio 1g: 30mL, and rotating speed is 7000r emulsification pretreatment 30min; Regulate pH4.5 with the hydrochloric acid solution of 1mol/L, add 0.2g cellulase (enzyme activity is 30,000 U/g) 50 DEG C, enzymolysis 8h; Be warming up to 60 DEG C, adjust pH to 8, then add 0.04g alkali protease (enzyme activity is 200,000 U/g) enzymolysis 1h, go out enzyme; The centrifugal 10min of 10000r/min, collects clear liquid, filters, and with 4 times of volume 98% alcohol settling 1h, standing separation, drying precipitatedly obtains soluble dietary fiber 0.72g.
After testing: the soluble dietary fiber purity 81.6% obtained.
Embodiment 3
In Example 1, the defatted grape seed powder 2.0g of step (1), adds pure water by solid-liquid ratio 1g: 25mL, and rotating speed is 7000r emulsification pretreatment 30min; Regulate pH4.5 with the hydrochloric acid solution of 1mol/L, add 0.04g cellulase (enzyme activity is 30,000 U/g) 45 DEG C, enzymolysis 9h; Be warming up to 55 DEG C, adjust pH to 8, then add 0.008g alkali protease (enzyme activity is 200,000 U/g) enzymolysis 1.5h, go out enzyme; The centrifugal 10min of 8000r/min, collects clear liquid, filters, and with 4 times of volume 98% alcohol settling 1h, standing separation, drying precipitatedly obtains soluble dietary fiber 0.15g.
After testing: the soluble dietary fiber purity 82.6% obtained.
Claims (4)
1. a preparation method for grape pip soluble dietary fiber, is characterized in that, described method comprises the steps:
1) preparation of defatted grape seed powder:
A) be 10KPa-20KPa by the grape skin after wine brewing in vacuum, temperature is 40 DEG C--dry under the cryogenic vacuum condition of 80 DEG C, drying time is 3-72 hour, through screening, grape pip is separated with Grape Skin, grape pedicel after drying, and make dried grape pip moisture content≤10%, clump count≤1000;
B) by grease in dried grape pip cold press removing grape pip, become the grape pip dregs of rice, in cold pressing process, make the temperature of grape pip be no more than 85 DEG C;
C) by the grape pip dregs of rice through pulverizer coarse crushing, be crushed to≤grape pip the dregs of rice particle of 1cm;
D) grape pip dregs of rice particle is worn into grape pip fine powder through flour mill;
E) grape pip fine powder dressing sieve machine is filtered out 80-30 object fine powder, be defatted grape seed powder;
2) preparation of soluble dietary fiber: take defatted grape seed powder, adds 20-30 times of weighing body ponding, emulsification pretreatment; Adjust pH4.5, add 2.0% cellulase, 40-50 DEG C, enzymolysis 8-10h; Be warming up to 50-60 DEG C, adjust pH to 8, then add the alkali protease enzymolysis 1-3h of 0.4%, go out enzyme; The centrifugal 10-20min of 6000-10000r/min, collects clear liquid, and filter, with 4 times of volume 98% alcohol settling 1h, standing separation, dry sediment obtains soluble dietary fiber.
2. the preparation method of a kind of grape pip soluble dietary fiber of method according to claim 1, is characterized in that: described step 2) in, in described emulsification pretreatment step, the rotating speed of emulsification pretreatment is 7000r, and the time of emulsification pretreatment is 30min.
3. the preparation method of a kind of grape pip soluble dietary fiber of method according to claim 1, is characterized in that: described step 2) in, the enzyme activity of described cellulase is 30,000 U/g, and the enzyme activity of described alkali protease is 200,000 U/g.
4. the preparation method of a kind of grape pip soluble dietary fiber of method according to claim 1, it is characterized in that: described step 2) in, in drying steps, drying mode is at least one in forced air drying, pneumatic conveying drying, spraying dry, vacuum drying and freeze drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510878888.3A CN105520156A (en) | 2015-12-04 | 2015-12-04 | Preparation method of grape seed soluble dietary fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510878888.3A CN105520156A (en) | 2015-12-04 | 2015-12-04 | Preparation method of grape seed soluble dietary fiber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105520156A true CN105520156A (en) | 2016-04-27 |
Family
ID=55763067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510878888.3A Pending CN105520156A (en) | 2015-12-04 | 2015-12-04 | Preparation method of grape seed soluble dietary fiber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105520156A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036920A (en) * | 2016-05-25 | 2016-10-26 | 广州聚注专利研发有限公司 | Method for extracting water-soluble dietary fibers from pennisetum hydridum |
| CN107981364A (en) * | 2017-11-29 | 2018-05-04 | 南京财经大学 | A kind of method that dietary fiber of high activity is prepared in the leftover bits and pieces from bamboo shoots |
| CN109874999A (en) * | 2019-03-22 | 2019-06-14 | 新疆海瑞盛生物工程股份有限公司 | Synchronous grape pip powder, preparation and the dedicated unit for extracting protein and dietary fiber |
| CN111642752A (en) * | 2020-05-09 | 2020-09-11 | 天津芸熙生物技术有限公司 | Extraction method of soluble dietary fiber of grapes |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6230200A (en) * | 1985-07-31 | 1987-02-09 | 大橋 健作 | Cosmetic soap having solid particle compounded therein |
| CN101077197A (en) * | 2007-06-26 | 2007-11-28 | 浙江大学 | Technology for preparing functional dietary fiber through waste bamboo shell enzymolysis |
| CN101283760A (en) * | 2008-05-27 | 2008-10-15 | 中国食品发酵工业研究院 | A method for extracting and preparing meal fibre from the peach dregs |
| CN102578594A (en) * | 2012-02-24 | 2012-07-18 | 怀来葡缇泉葡萄籽科技开发有限公司 | Method for preparing grapestone dietary fiber powder by taking grapestone as raw material and product of grapestone dietary fiber powder |
| CN103181570A (en) * | 2013-03-14 | 2013-07-03 | 甘肃省敦煌种业股份有限公司 | Method for extracting dietary fiber |
-
2015
- 2015-12-04 CN CN201510878888.3A patent/CN105520156A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6230200A (en) * | 1985-07-31 | 1987-02-09 | 大橋 健作 | Cosmetic soap having solid particle compounded therein |
| CN101077197A (en) * | 2007-06-26 | 2007-11-28 | 浙江大学 | Technology for preparing functional dietary fiber through waste bamboo shell enzymolysis |
| CN101283760A (en) * | 2008-05-27 | 2008-10-15 | 中国食品发酵工业研究院 | A method for extracting and preparing meal fibre from the peach dregs |
| CN102578594A (en) * | 2012-02-24 | 2012-07-18 | 怀来葡缇泉葡萄籽科技开发有限公司 | Method for preparing grapestone dietary fiber powder by taking grapestone as raw material and product of grapestone dietary fiber powder |
| CN103181570A (en) * | 2013-03-14 | 2013-07-03 | 甘肃省敦煌种业股份有限公司 | Method for extracting dietary fiber |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036920A (en) * | 2016-05-25 | 2016-10-26 | 广州聚注专利研发有限公司 | Method for extracting water-soluble dietary fibers from pennisetum hydridum |
| CN107981364A (en) * | 2017-11-29 | 2018-05-04 | 南京财经大学 | A kind of method that dietary fiber of high activity is prepared in the leftover bits and pieces from bamboo shoots |
| CN109874999A (en) * | 2019-03-22 | 2019-06-14 | 新疆海瑞盛生物工程股份有限公司 | Synchronous grape pip powder, preparation and the dedicated unit for extracting protein and dietary fiber |
| CN111642752A (en) * | 2020-05-09 | 2020-09-11 | 天津芸熙生物技术有限公司 | Extraction method of soluble dietary fiber of grapes |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101948897B (en) | Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn | |
| CN101617787B (en) | Process for continuously extracting a plurality of products from highland barley | |
| CN104543259A (en) | Processing technique of fructus momordicae juice fine powder | |
| CN103571609A (en) | Method for synchronously preparing high-stability rice bran oil and rice bran protein | |
| CN104187456A (en) | Technical method for extracting dietary fiber from pear residue | |
| CN105520156A (en) | Preparation method of grape seed soluble dietary fiber | |
| CN105166888A (en) | Ginseng and pilose antler tea seed polypeptide lozenge and preparation method therefor | |
| CN103361333B (en) | The preparation method of a kind of bromelain lixiviating solution and stability protective agent used thereof | |
| CN103897938A (en) | Oat yellow wine and brewing technology thereof | |
| CN104928018A (en) | Method for extracting tea-seed oil by microwave expansion assisted biological enzymolysis | |
| CN105166939A (en) | Tea seed polypeptide lozenge and preparation method therefor | |
| CN104480171A (en) | Method for extracting polypeptide from velvet bean residue | |
| CN104140471A (en) | Method for preparing yam high purity polysaccharide from yam food processing leftovers | |
| CN103421612A (en) | Method for manufacturing oil by using tea oil residues | |
| CN102787013B (en) | Method for fermenting and extracting corn oil by microorganisms | |
| CN106900977A (en) | The method that feed is prepared using shrimping beam trawl | |
| CN107557406A (en) | A kind of method that xylo-oligosaccharide is extracted from corncob | |
| CN104840587A (en) | Method for extracting longan seed polyphenol | |
| CN102757857B (en) | Wheat-germ oil extracting method | |
| CN109170885A (en) | A method of extracting ginseng effective component | |
| CN103621929A (en) | Method for preparing sugar-free and smell-free pumpkin powder | |
| CN106432529B (en) | A kind of preparation method of high-purity rice bran polysaccharide | |
| CN102742854A (en) | New process for extracting dietary fibers from corn germ meal | |
| CN104762356A (en) | Pumpkin leaf polysaccharide and polypeptide and preparation method thereof | |
| CN104745425A (en) | Yellow wine lees beverage and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160427 |
|
| WD01 | Invention patent application deemed withdrawn after publication |