CN106432529B - A kind of preparation method of high-purity rice bran polysaccharide - Google Patents
A kind of preparation method of high-purity rice bran polysaccharide Download PDFInfo
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- CN106432529B CN106432529B CN201611044383.8A CN201611044383A CN106432529B CN 106432529 B CN106432529 B CN 106432529B CN 201611044383 A CN201611044383 A CN 201611044383A CN 106432529 B CN106432529 B CN 106432529B
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses a kind of preparation methods of high-purity rice bran polysaccharide, this method are as follows: pass through pretreatment, the defatted rice bran dregs of rice after obtaining impurity elimination, activated polysaccharide, as raw material, using pressured activation, atmospheric hot-water extraction, film Purification by filtration, concentration, vacuum microwave drying, it crushes, obtains the rice bran polysaccharide powder of high-purity.The preprocess method, it is characterised in that use following steps: defatted rice bran and aqueous citric acid solution are with the mass ratio of 1:5-10, and cleaning, filtering, abandoning filtrate remove acid soluble impurities；Maturity period and the mixing of button phase ganoderma lucidum thallus are planted, 0-5 DEG C of low-temperature homogenate obtains ganoderma lucidum ferment；The defatted rice bran dregs of rice after removal of impurities are added in ganoderma lucidum ferment, mixed enzymolysis of milling reaction discharges active polysaccharide.The present invention solves the problems, such as that the current existing impurity of rice bran polysaccharide production is more, recovery rate is low, and the rice bran polysaccharide of production, recovery rate is high, and impurity is few, and purity, mouthfeel is superior to traditional extraction process.
Description
Technical field
The present invention provides a kind of preparation method of high-purity rice bran polysaccharide, belongs to food processing technology field.
Background technique
Rice bran is the significant by-products of paddy processing, and China's annual output is more than 10,000,000 tons, and the world rice bran yield Ye Ju is first
Position, is a kind of renewable resource having a large capacity and a wide range, is currently used primarily in animal feed, added value of product is extremely low.The United Nations's industry
Rice bran is become a kind of raw material underused by development institution, and the further development and utilization of rice bran resource can be used as food
Industry provides raw materials for production abundant.
There is a plurality of types of polysaccharide in rice bran, and component and structure are also different, have multiple biological activities.No
The same research worker title code name different to extracted rice bran polysaccharide, such as the RBS of Eichi extraction, Takeo etc. are extracted
RDP, RON, RBF-P, RBF-PM of the extractions such as Kimitoshi, the RBG-3 of the preparations such as Lamkooh, the preparations such as Kado
RBHB, RBHA etc. of the preparations such as RBSR-01 and Hu Guohua.Many research datas show that rice bran polysaccharide increases antitumor, immune
By force, bacterial-infection resisting and hypoglycemic etc. also bioactivity with higher.
It extracts water-soluble polysaccharide from rice bran at present mainly directly to be extracted with water as solvent, extracting method has: passing through enzyme process, height
The method that pressure, ultrasound, microwave method auxiliary water extract.When with aqueous solvent extracting method, low efficiency is extracted under normal pressure；Separately
Outside, under the high-temperature and high-pressure conditions for being pressurised into 100MPa or more, under neutrallty condition, yield is lower close to room temperature；In addition 130
DEG C, under the high-temperature and high-pressure conditions of 20MPa, under neutrallty condition, if not having to the technologies such as extruder, water solubility cannot be effectively obtained
Polysaccharide；Under high temperature and pressure neutrallty condition, it is combined with enzyme process, complex treatment process；Ultrasonic wave and microwave assisted aqueous extraction mention rice bran polysaccharide
Recovery rate improves limited；Pressurize acidity extraction, and easily causing polysaccharide hydrolysis leads to low molecule quantization, influences active polysaccharide；Normal pressure
High-alkali extracting method is heated, is commonly used in NaOH or KOH and common HCl, there are low efficiency, time-consuming, and pigment impurity is more, process
It is cumbersome.It can be seen that existing rice bran polysaccharide extracting method, does not carry out effective pretreatment removal of impurities to raw material and polysaccharide discharges,
Extraction efficiency, product purity and taste quality are affected, there are obvious shortcomings.
Summary of the invention
The present invention provides a kind of preparation methods of high-purity rice bran polysaccharide.In order to overcome rice bran polysaccharide system in the prior art
Low for there are extraction efficiencies, purity is low, and the deficiency of taste quality difference, the present invention provides a kind of preparation side of high-purity rice bran polysaccharide
Method.
First technical problem to be solved by this invention is to provide the method for acid soluble impurities in defatted rice bran；
The invention solves second technical problem be provide removal defatted rice bran in big molecular impurity method；
The invention solves third technical problem be to provide the method for releasing of rice bran polysaccharide.
In order to solve the first technical problem mentioned above, the present invention uses aqueous citric acid solution cleaning and degreasing rice bran, and filter is abandoned in filtering
Liquid removes acid soluble impurities；
It is described to be cleaned using aqueous citric acid solution, aqueous citric acid solution used, PH 2-6, concentration 0.1-0.3mol/
L；PH acid-base accommodation, acidification acid can include: H2SO4、HNO3Equal inorganic acids, alkali are the inorganic bases such as NaOH.
Described, defatted rice bran: aqueous citric acid solution (w/w) ratio is 1:5-10, scavenging period 20-40min.
To solve second technical problem, the present invention is digested using ganoderma lucidum ferment, removes big molecular impurity；
The ganoderma lucidum ferment be ganoderma lucidum Mixed Microbes body hypothermia homogenate obtain product, ganoderma lucidum mix thallus be maturity period ganoderma lucidum and
Button phase ganoderma lucidum mixes thallus, weight ratio 1-3:1；Homogenized temperature is 0-5 DEG C；
The ganoderma lucidum ferment is milled mixed enzymolysis condition are as follows: enzymatic hydrolysis hybrid mode is mechanical disruption, hydrolysis temperature 50-60
DEG C, time 30-120min, in terms of 1kg defatted rice bran, starch enzyme dosage is 100-300U in crude enzyme liquid.
For solve third technical problem, the present invention provides mechanical disruption, ganoderma lucidum rhzomorph enzymatic hydrolysis two kinds of delivery modes, two
Person acts synergistically, and plays the role of promoting rice bran polysaccharide release jointly.
The present invention is digested by pre-treatment using citric acid solution cleaning, ganoderma lucidum ferment, and removal impurity is more thorough, passes through
Mechanical disruption cooperates with ganoderma lucidum ferment enzymatic hydrolysis, discharges active polysaccharide, increases recovery rate, it is low, pure to solve rice bran polysaccharide recovery rate
Spend not high and poor taste status.Rice bran polysaccharide prepared by the present invention, recovery rate is high, impurity is few, and purity, mouthfeel are superior to tradition
Extracting method.
Specific embodiment
The present invention will be described in further detail with reference to embodiments.
By pretreatment, to remove impurity, the defatted rice bran dregs of rice of activated polysaccharide are raw material, the pressured activation polysaccharide of use,
Pressure is 100kPa, and temperature is 120 DEG C, time 30min；Atmospheric hot-water extracts, defatted rice bran: water ratio (w/w) is 1:10；
Extracting temperature is 90 DEG C, and extraction time is 2 times, each 1h, and film Purification by filtration, concentration, vacuum microwave drying crush, and is formed high
The rice bran polysaccharide powder of purity.
The preprocess method, it is characterised in that use following steps: defatted rice bran and aqueous citric acid solution (soda acid tune
Section PH is 2-6, concentration 0.1-0.3mol/L) with the mass ratio of 1:5-10, it cleans (20-40min), filtrate is abandoned in filtering, is removed
Acid soluble impurities；Maturity period and button phase ganoderma lucidum thallus (weight ratio 1-3:1) mixing are planted, 0-5 DEG C of low-temperature homogenate obtains ganoderma lucidum
Ferment；The defatted rice bran dregs of rice after removal of impurities are added in ganoderma lucidum ferment, mixed enzymolysis of milling reaction discharges active polysaccharide, hydrolysis temperature
It is 50-60 DEG C, time 30-120min, in terms of 1kg defatted rice bran, starch enzyme dosage is 100-300U in crude enzyme liquid.
Embodiment 1
(1) defatted rice bran and aqueous citric acid solution (H2SO4Adjusting PH is 2, concentration 0.3mol/L) with the quality of 1:10
Than cleaning (20min), filtrate is abandoned in filtering, removes acid soluble impurities；
(2) maturity period and button phase ganoderma lucidum thallus (weight ratio 3:1) mixing are planted, 0 DEG C of low-temperature homogenate obtains ganoderma lucidum
Ferment；
(3) the defatted rice bran dregs of rice after removal of impurities are added in ganoderma lucidum ferment, mixed enzymolysis of milling reaction discharges active polysaccharide, enzyme
Solving temperature is 60 DEG C, time 80min, and in terms of 1kg defatted rice bran, starch enzyme dosage is 200U in crude enzyme liquid；
(4) mixed solution is added in pressurised extraction tank, activated polysaccharide, pressure 100kPa, and temperature is 120 DEG C, and the time is
30min；
(5) defatted rice bran mixed solution is added in normal pressure extractor, and adjust defatted rice bran: water ratio (w/w) is 1:10, is mentioned
Taking temperature is 90 DEG C, and extraction time is 2 times, each 1h, the material centrifugation after decoction is removed slag, supernatant liquid filtering, obtains rice bran
Polysaccharide extraction liquid；
(6) rice bran polysaccharide extracting solution, after ceramic membrane equipment membrane separation concentration, filtrate imports storage tank；
(7) vacuum microwave drying crushes, and rice bran polysaccharide powder is made；
(8) recovery rate is 4.6%, and purity 82%, mouthfeel is slightly sweet.
Embodiment 2
(1) defatted rice bran and aqueous citric acid solution (HNO3Adjusting PH is 3, concentration 0.2mol/L) with the mass ratio of 1:8,
It cleans (30min), filtrate is abandoned in filtering, removes acid soluble impurities；
(2) maturity period and button phase ganoderma lucidum thallus (weight ratio 1:1) mixing are planted, 3 DEG C of low-temperature homogenates obtain ganoderma lucidum
Ferment；
(3) the defatted rice bran dregs of rice after removal of impurities are added in ganoderma lucidum ferment, mixed enzymolysis of milling reaction discharges active polysaccharide, enzyme
Solving temperature is 55 DEG C, time 120min, and in terms of 1kg defatted rice bran, starch enzyme dosage is 100U in crude enzyme liquid；
(4) mixed solution is added in pressurised extraction tank, activated polysaccharide, pressure 100kPa, and temperature is 120 DEG C, and the time is
30min；
(5) defatted rice bran mixed solution is added in normal pressure extractor, and adjust defatted rice bran: water ratio (w/w) is 1:10, is mentioned
Taking temperature is 90 DEG C, and extraction time is 2 times, each 1h, the material centrifugation after decoction is removed slag, supernatant liquid filtering, obtains rice bran
Polysaccharide extraction liquid；
(6) rice bran polysaccharide extracting solution, after ceramic membrane equipment membrane separation concentration, filtrate imports storage tank；
(7) vacuum microwave drying crushes, and rice bran polysaccharide powder is made；
(8) recovery rate is 4.1%, and purity 72%, mouthfeel is slightly sweet.
Embodiment 3
(1) defatted rice bran and aqueous citric acid solution (it is 6 that NaOH, which adjusts PH, concentration 0.1mol/L) be with the mass ratio of 1:5,
It cleans (40min), filtrate is abandoned in filtering, removes acid soluble impurities；
(2) maturity period and button phase ganoderma lucidum thallus (weight ratio 2:1) mixing are planted, 5 DEG C of low-temperature homogenates obtain ganoderma lucidum
Ferment；
(3) the defatted rice bran dregs of rice after removal of impurities are added in ganoderma lucidum ferment, mixed enzymolysis of milling reaction discharges active polysaccharide, enzyme
Solving temperature is 55 DEG C, time 30min, and in terms of 1kg defatted rice bran, starch enzyme dosage is 300U in crude enzyme liquid；
(4) mixed solution is added in pressurised extraction tank, activated polysaccharide, pressure 100kPa, and temperature is 120 DEG C, and the time is
30min；
(5) defatted rice bran mixed solution is added in normal pressure extractor, and adjust defatted rice bran: water ratio (w/w) is 1:10, is mentioned
Taking temperature is 90 DEG C, and extraction time is 2 times, each 1h, the material centrifugation after decoction is removed slag, supernatant liquid filtering, obtains rice bran
Polysaccharide extraction liquid；
(6) rice bran polysaccharide extracting solution, after ceramic membrane equipment membrane separation concentration, filtrate imports storage tank；
(7) vacuum microwave drying crushes, and rice bran polysaccharide powder is made；
(8) recovery rate is 3.6%, purity 66%, and mouthfeel is micro- salty.
Claims (4)
1. a kind of preparation method of high-purity rice bran polysaccharide, it is characterised in that by pretreatment, obtain removal impurity, activated polysaccharide
The defatted rice bran dregs of rice afterwards, as raw material, pressured activation, atmospheric hot-water is extracted, and after purification, vacuum microwave is dry for membrane separation concentration
It is dry, it crushes and high-purity rice bran polysaccharide is made, the preprocessing process uses following steps:
(1) defatted rice bran and aqueous citric acid solution, acid-base accommodation pH is 2-6, concentration 0.1-0.3mol/L, with the weight of 1:5-10
Ratio is measured, 20-40min is cleaned, filtrate is abandoned in filtering, removes acid soluble impurities；(2) maturity period and button phase Ganoderma Lucidum weight are planted
Amount is mixed than 1-3:1,0-5 DEG C of low-temperature homogenate, obtains ganoderma lucidum ferment；(3) the defatted rice bran dregs of rice after removal of impurities are added in ganoderma lucidum ferment,
Mixed enzymolysis of milling reaction discharges active polysaccharide, and hydrolysis temperature is 50-60 DEG C, time 30-120min, with 1kg defatted rice bran
It counts, starch enzyme dosage is 100-300U in crude enzyme liquid.
2. high-purity rice bran polysaccharide preparation method according to claim 1, which is characterized in that in the pre-treatment step:
It is cleaned in step (1) using aqueous citric acid solution, aqueous citric acid solution used, pH 2-6, concentration 0.1-0.3mol/L；pH
With acid-base accommodation, acid is inorganic acid H2SO4、HNO3, alkali is inorganic base NaOH；Defatted rice bran: aqueous citric acid solution weight ratio is 1:
5-10, scavenging period 20-40min.
3. high-purity rice bran polysaccharide preparation method according to claim 1, which is characterized in that in the pre-treatment step:
Enzyme used is the ganoderma lucidum ferment of ganoderma lucidum thallus release in step (2), is obtained for mixing Ganoderma Lucidum body hypothermia homogenate, the ganoderma lucidum
Thallus is maturity period ganoderma lucidum thallus and button phase ganoderma lucidum thallus Mixed Microbes body, maturity period ganoderma lucidum thallus: button phase Ganoderma Lucidum weight
For amount than being 1-3:1, low-temperature homogenate temperature is 0-5 DEG C.
4. a kind of preparation method of high-purity rice bran polysaccharide according to claim 1, which is characterized in that this method are as follows:
To remove impurity by pretreatment, the defatted rice bran dregs of rice of activated polysaccharide are raw material, the pressured activation polysaccharide of use, pressure
For 100kPa, temperature is 120 DEG C, time 30min；Atmospheric hot-water extracts, defatted rice bran: water weight ratio is 1:10；Temperature is
90 DEG C, extraction time is 2 times, each 1h, and film Purification by filtration, concentration, vacuum microwave drying crush, forms the rice bran of high-purity
Polysaccharide powder.
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| CN111548427A (en) * | 2019-02-12 | 2020-08-18 | 湖北洪森生物科技有限公司 | Preparation method of high-activity rice polysaccharide powder |
| CN109734823A (en) * | 2019-03-06 | 2019-05-10 | 武汉轻工大学 | A kind of cationization rice bran polysaccharide and preparation method thereof and genophore |
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| CN103484250A (en) * | 2013-08-16 | 2014-01-01 | 山东省食品发酵工业研究设计院 | Method for preparing red jujube essence and red jujube polysaccharides by using defective jujubes |
| CN104910293A (en) * | 2015-06-24 | 2015-09-16 | 雷邦斯生物技术（北京）有限公司 | Process for producing alginic acid |
| CN105327331A (en) * | 2015-10-15 | 2016-02-17 | 湖北洪森实业（集团）有限公司 | Composition for treating and preventing senile dementia and preparation method and application of composition for treating and preventing senile dementia |
| CN105935143A (en) * | 2016-06-29 | 2016-09-14 | 浙江蕴本生物科技有限公司 | Manufacturing method of lucid ganoderma ferment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103484250A (en) * | 2013-08-16 | 2014-01-01 | 山东省食品发酵工业研究设计院 | Method for preparing red jujube essence and red jujube polysaccharides by using defective jujubes |
| CN104910293A (en) * | 2015-06-24 | 2015-09-16 | 雷邦斯生物技术（北京）有限公司 | Process for producing alginic acid |
| CN105327331A (en) * | 2015-10-15 | 2016-02-17 | 湖北洪森实业（集团）有限公司 | Composition for treating and preventing senile dementia and preparation method and application of composition for treating and preventing senile dementia |
| CN105935143A (en) * | 2016-06-29 | 2016-09-14 | 浙江蕴本生物科技有限公司 | Manufacturing method of lucid ganoderma ferment |
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