CN101948897B - Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn - Google Patents
Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn Download PDFInfo
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Abstract
The invention belongs to the deep processing technology of shinyleaf yellowhorn, in particular to a method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn. The method comprises the following steps of: taking the kernels of shinyleaf yellowhorn, crushing the shinyleaf yellowhorn kernels, and adding water to swell; continuously treating by using microwaves, hydrolyzing by using alkali protease, separating three phases by using neutral protease, flavourzyme, cellulase and hemicellulase, concentrating through ultrafiltration by using an ultrafiltration membrane, drying the concentrated solution, and vacuum-drying and crushing the hydrolyzing residue to obtain high-grade animal feed rich in protein, fat and cellulose. In a word, the invention overcomes various defects caused by the traditional vegetable oil preparation process and adopts a brand new process for furthest extracting pollution-free shinyleaf yellowhorn oil and simultaneously acquiring shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide. The whole processing process reflects continuity, rapidness and mechanization, and production efficiency is greatly improved.
Description
Technical field
The invention belongs to the Wood of Shinyleaf Yellowhorn deep process technology, be specifically related to a kind of method of from Wood of Shinyleaf Yellowhorn, extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide.
Background technology
Wood of Shinyleaf Yellowhorn is Sapindaceae Wood of Shinyleaf Yellowhorn platymiscium, has another name called pawpaw, civil official and really is machaka or arbor, originates in northern China, grows in barren hill cheuch and the hilly country at 52 ~ 2260 meters of height above sea level.Its seed oil length 30.4% is planted benevolence oil length 66.39% after the peeling, contain protein 25.75%.Unsaturated fatty acid content accounts for 36.9% oleic acid up to 94% linolic acid to account for 57.16% is the table oil more abundanter than sweet oil, tea oil nutrition in its oil., its protein content is also very abundant, it is the higher vegetable-protein kind of a kind of nutritive value, also contain more active polysaccharide in the Wood of Shinyleaf Yellowhorn, molecular-weight average also is a kind of and the functional product of tool exploitation at 10-15 about ten thousand.Other cold-resistant drought-enduring barren-resistant can extensively the plantation in the hilly country, deserted mountain of Wood of Shinyleaf Yellowhorn strong adaptability..Exploitation Wood of Shinyleaf Yellowhorn resource not only can planting trees on barren hills be checked winds and fixed drifting sand but also can be created economic worth.The main method of prepairng vegetable oil is traditional milling process and extraction at present.Milling process technique is simple, support equipment is few, the flavour that can keep product, but this method residual oil content high (7～10%), labour intensity is large, power consumption is large, cost is high, and the cake protein qualitative change degree after the squeezing is high, can only be used as fertilizer, protein utilization rate is low, causes the waste of a large amount of quality plant albumen.Lixiviation process production efficiency height and residual oil content are low, but this method equipment is many, the investment large; The crude oil complicated component needs refining treatment, and the oil product flavor loss is large, and the oil colours pool of leaching is darker, also needs further refining; With an organic solvent increase the loaded down with trivial details property of technique, reduced the security of producing, caused the pollution of environment; Minim organic solvent residual in the processed oil is unfavorable to human health.Protein polypeptide is the protein fragments that two or more amino acid connect into by peptide bond, and it is the ultimate constituent of body tissue cell.Since the importance of biological bioactive peptide in food and healthcare products was familiar with, the employing enzyme process extracts biologically active peptides from plant resources research was also more and more extensive.The Wood of Shinyleaf Yellowhorn protein peptide has important physiological function, and it can remove interior free yl, reaches effect anti-oxidant, that prolong cell survival.Its property digested and assimilated is good simultaneously, food safety is high, be applied to be regarded as in baby and children's formula food, diet food, arsenic and the dietetic food " emerging nutritive health-care source " and " functional factor that has development potentiality " so can be used as nutrition-fortifying agent.The Wood of Shinyleaf Yellowhorn polysaccharide has several functions, can blood fat reducing, control blood sugar, strengthening immunity, and anti-ly treat constipation, improve intestinal microenvironment etc., be widely used in medicine and field of health care food.The angle development that the utilization of Wood of Shinyleaf Yellowhorn component should extract, fully utilize towards total composition.Thus, come efficient, industrialization to produce high-quality, the shinyleaf yellowhorn oil of nutritive health-care and the method for Wood of Shinyleaf Yellowhorn protolysate peptide in the urgent need to developing a kind of new coproduction reparation technology.
Application number is 200910019388.9 to disclose a kind of method of extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, method is to operate as follows: be 1: 4～8 to pour in the colloidal mill and pulverize Wood of Shinyleaf Yellowhorn Renhe water by solid-liquid ratio (g/mL) 1), pulverize Wood of Shinyleaf Yellowhorn benevolence to the 70-160 order, collect slurries; 2) pH regulator with the slurries of milled is 3.8-5.8, adds the cellulase of Wood of Shinyleaf Yellowhorn benevolence quality 0.5-2.5%, in 50-60 ℃ of water-bath enzymolysis 3-8 hour; 3) enzymolysis solution is centrifugal, 4000-6000 rev/min, centrifugal 20-30 minute; Centrifugal complete after, draw upper strata edible vegetable oil; 4) pH with lower floor's enzymolysis solution is adjusted to 6.8-7.3, adds the Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence quality 1.8～2.5%, and reaction is 2.5-3.5 hour in 50-60 ℃ of water-bath; 5) with enzymolysis solution at 4500-6000 rev/min of centrifugal 30-35 minute, the yellow edible vegetable oil in sucking-off upper strata can merge with the edible vegetable oil of the sucking-off first time, also can use separately.Aforesaid method has mentioned that the use enzymolysis process extracts, but the enzyme that uses is single, and the extracting substance kind that obtains is few, and of poor quality.
Summary of the invention
The purpose of this invention is to provide a kind of method of from Wood of Shinyleaf Yellowhorn, extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide.
The present invention is realized that by following scheme a kind of method of extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn may further comprise the steps:
1) get the benevolence of Wood of Shinyleaf Yellowhorn, Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds simultaneously Wood of Shinyleaf Yellowhorn benevolence weight 20-30% stirs, and places and makes its swelling in 1-2 hour;
2) the Wood of Shinyleaf Yellowhorn benevolence swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and process, the frequency of microwave is 2400-2500MHz, and power is 60KW, 100 ℃-121 ℃ of temperature, transfer rate is 2.0-4.0m/min, and the time is 1.5-3.0 min;
3) the Wood of Shinyleaf Yellowhorn benevolence of microwave treatment is added 5-6 times of water of its weight, regulate pH value to 8.5-9.5, add the Sumizyme MP of the 1-2% of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value at 8.5-9.5 under the 45-60 ℃ of condition, be hydrolyzed 2-3 hour;
4) then at pH value 6.5-7.0, under the 45-60 ℃ of condition, each adds neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 2-4 hour of the 0.3-0.5% of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 80-100 ℃, stirs 10-20min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains respectively oil phase, water and unhydrolysed residue solid phase.Oil phase is shinyleaf yellowhorn oil, and water is mainly the Wood of Shinyleaf Yellowhorn protolysate peptide, and solid phase is that the unhydrolysed residue of Wood of Shinyleaf Yellowhorn contains part grease, albumen, Mierocrystalline cellulose.Unsaturated fatty acid content is higher than 93% in the shinyleaf yellowhorn oil that obtains;
7) with 6) communicated the first ultra-filtration membrane ultrafiltration and concentration of molecular weight 4000-6000 of the described solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains macromole Wood of Shinyleaf Yellowhorn polysaccharide content about 10-20%, and then removes residual sugar and be concentrated into protein peptide content about 15-20% by the nanofiltration of molecular weight 300-500;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 12-15MPa, inlet temperature: 155-185 ℃, temperature of outgoing air: 75-85 ℃, the Wood of Shinyleaf Yellowhorn polysaccharide molecular-weight average 10-15 ten thousand that obtains after the drying, water content is less than 5%, and purity is greater than more than 90%;
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 12-15MPa, inlet temperature: 155-185 ℃, temperature of outgoing air: 75-85 ℃, the protein peptide powder water content that obtains after the drying is less than 5%, and purity is greater than more than 85%;
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
The invention provides a kind of shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn proteolysis Gly-His-Lys of high-quality, it can be widely used in the industries such as food and healthcare products.Its concrete advantage is as follows: 1, product of the present invention is to make with extra care the all-natural product that forms by the civil official's kernel that is rich in grease and albumen through scientific approach, uses any organic solvent in making processes, belongs to pure natural, high nutrition, health food.2, pure natural shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and proteolysis Gly-His-Lys have very good market comsupton prospect, can be used as the batching of food or raw materials for food industry and medicine and healthcare products etc., be widely used in beverage, cold drink, candy, protective foods, bake, the industries such as snackfoods, medicine, preparation becomes various nutrition and functional food and medicine with deep processing.Its product has very large market capacity, and its economic and social benefits also will increase substantially thereupon.3, preparation technology of the present invention adopts microwave, extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction, and Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate Gly-His-Lys have improved extract yield, have reduced extraction time, and have not had waste discharge, reduce cost, increase economic efficiency.
In a word: the present invention overcomes the various shortcoming that traditional vegetables oil reparation technology brings, and adopts brand-new technique, a kind of not only pollution-free extraction shinyleaf yellowhorn oil to greatest extent is provided, and has obtained simultaneously Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide.All the course of processing has embodied continuity, quick and mechanize, has greatly improved production efficiency.
Embodiment
Embodiment 1, and a kind of method of extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn may further comprise the steps:
1) Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds simultaneously Wood of Shinyleaf Yellowhorn benevolence weight 20% stirs, and places and makes its swelling in 2 hours;
2) civil official's kernel swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and process, the frequency of microwave is 2400 MHz, and power is 60KW, and 110 ℃ of temperature, transfer rate are 2.0m/min, and the time is 3.0 min;
3) civil official's kernel of microwave treatment is added 5 times of water of its weight, regulate pH value to 8.5, add 1% Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value 8.5 under 50 ℃ of conditions, be hydrolyzed 3 hours;
4) then under 7.0,50 ℃ of conditions of pH value, each adds 0.3% neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 4 hours of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 90 ℃, stirs 20min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains respectively oil phase, water and unhydrolysed residue solid phase.Oil phase is shinyleaf yellowhorn oil, and water is mainly the Wood of Shinyleaf Yellowhorn protolysate peptide, and solid phase is that the unhydrolysed residue of Wood of Shinyleaf Yellowhorn contains part grease, albumen, Mierocrystalline cellulose.Unsaturated fatty acid content 94% in the shinyleaf yellowhorn oil that obtains.Wherein linolic acid accounts for 37% oleic acid and accounts for 57%;
7) with 6) the described solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains macromole Wood of Shinyleaf Yellowhorn polysaccharide content about 10% by the ultra-filtration membrane ultrafiltration and concentration of molecular weight 4000, and then removes residual sugar and be concentrated into protein peptide content about 15% by the nanofiltration of molecular weight 300;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 15 MPa, inlet temperature: 185 ℃, temperature of outgoing air: 85 ℃, the Wood of Shinyleaf Yellowhorn polysaccharide molecular-weight average 100,000 that obtains after the drying, water content 5%, purity 91%.
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 12 MPa, and inlet temperature: 155 ℃, temperature of outgoing air: 85 ℃, the protein peptide powder water content 4% that obtains after the drying, purity 88%.
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
Embodiment 2
1) Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds simultaneously Wood of Shinyleaf Yellowhorn benevolence weight 30% stirs, and places and makes its swelling in 1 hour;
2) civil official's kernel swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and process, the frequency of microwave is 2500MHz, and power is 60KW, and 121 ℃ of temperature, transfer rate are 4.0m/min, and the time is 1.5 min.
3) civil official's kernel of microwave treatment is added 5 times of water of its weight, regulate pH value to 9.0, add 2% Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value 9.0 under 55 ℃ of conditions, be hydrolyzed 2 hours;
4) then under 7.0,55 ℃ of conditions of pH value, each adds 0.5% neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 2 hours of Wood of Shinyleaf Yellowhorn benevolence weight.
5) with 4) the described liquid that obtains is warming up to 95 ℃, stirs 10min go out enzyme, breakdown of emulsion.
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains respectively oil phase, water and unhydrolysed residue solid phase.Oil phase is shinyleaf yellowhorn oil, and water is mainly the Wood of Shinyleaf Yellowhorn protolysate peptide, and solid phase is that the unhydrolysed residue of Wood of Shinyleaf Yellowhorn contains part grease, albumen, Mierocrystalline cellulose.Unsaturated fatty acid content 95% in the shinyleaf yellowhorn oil that obtains.Wherein linolic acid accounts for 37.% oleic acid and accounts for 58%.
7) with 6) the described solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains macromole Wood of Shinyleaf Yellowhorn polysaccharide content about 20% by the ultra-filtration membrane ultrafiltration and concentration of molecular weight 6000, and then removing residual sugar by the nanofiltration of molecular weight 500 and be concentrated into protein peptide content about 20%.
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 13 MPa, inlet temperature: 175 ℃, temperature of outgoing air: 75 ℃, the Wood of Shinyleaf Yellowhorn polysaccharide molecular-weight average 150,000 that obtains after the drying, water content 3%, purity 93%.
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 14MPa, and inlet temperature: 165 ℃, temperature of outgoing air: 75 ℃, the protein peptide powder water content 3.5% that obtains after the drying, purity 86%.
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
Embodiment 3, a kind of method of extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn may further comprise the steps:
1) get the benevolence of Wood of Shinyleaf Yellowhorn, Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds simultaneously Wood of Shinyleaf Yellowhorn benevolence weight 25% stirs, and places and makes its swelling in 1.5 hours;
2) the Wood of Shinyleaf Yellowhorn benevolence swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and process, the frequency of microwave is 2450MHz, and power is 60KW, and 110 ℃ of temperature, transfer rate are 3.0m/min, and the time is 2.0 min;
3) the Wood of Shinyleaf Yellowhorn benevolence of microwave treatment is added 5.5 times of water of its weight, regulate pH value to 9, add 1.5% Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value 9 under 50 ℃ of conditions, be hydrolyzed 2.5 hours;
4) then at pH value 6.5-7.0, under 50 ℃ of conditions, each adds 0.4% neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 3 hours of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 90 ℃, stirs 15min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains respectively oil phase, water and unhydrolysed residue solid phase.Oil phase is shinyleaf yellowhorn oil, and water is mainly the Wood of Shinyleaf Yellowhorn protolysate peptide, and solid phase is that the unhydrolysed residue of Wood of Shinyleaf Yellowhorn contains part grease, albumen, Mierocrystalline cellulose.Unsaturated fatty acid content is higher than 93% in the shinyleaf yellowhorn oil that obtains;
7) with 6) communicated the first ultra-filtration membrane ultrafiltration and concentration of molecular weight 5000 of the described solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains macromole Wood of Shinyleaf Yellowhorn polysaccharide content about 10-20%, and then removes residual sugar and be concentrated into protein peptide content about 15-20% by the nanofiltration of molecular weight 400;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 13MPa, inlet temperature: 170 ℃, temperature of outgoing air: 80 ℃, the Wood of Shinyleaf Yellowhorn polysaccharide molecular-weight average 140,000 that obtains after the drying, water content is less than 4.5%, and purity is greater than more than 95%;
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, its condition is: high-pressure pump pressure-controlling 14MPa, and inlet temperature: 170 ℃, temperature of outgoing air: 80 ℃, the protein peptide powder water content that obtains after the drying is less than 3%, and purity is greater than more than 90%;
10) with 6) describedly pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying for hydrolytic residue.
Claims (2)
1. method of extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn is characterized in that: may further comprise the steps:
1) Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds simultaneously Wood of Shinyleaf Yellowhorn benevolence weight 20-30% stirs, and places and makes its swelling in 1-2 hour;
2) the Wood of Shinyleaf Yellowhorn benevolence swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and process, the frequency of microwave is 2400-2500MHz, and power is 60KW, 100 ℃-121 ℃ of temperature, transfer rate is 2.0-4.0m/min, and the time is 1.5-3.0 min;
3) the Wood of Shinyleaf Yellowhorn benevolence after the microwave treatment is added 5-6 times of water of its weight, regulate pH value to 8.5-9.5, add the Sumizyme MP of the 1-2% of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value at 8.5-9.5 under the 45-60 ℃ of condition, be hydrolyzed 2-3 hour;
4) then at pH value 6.5-7.0, under the 45-60 ℃ of condition, each adds neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 2-4 hour of the 0.3-0.5% of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 80-100 ℃, stirs 10-20min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains respectively oil phase, water and unhydrolysed residue solid phase;
7) with 6) the described aqueous phase solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains the Wood of Shinyleaf Yellowhorn polysaccharide soln by the ultra-filtration membrane ultrafiltration and concentration of molecular weight 4000-6000, and then removes residual sugar and be concentrated into protein peptide content at 15-20% by the nanofiltration of molecular weight 300-500;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, and its condition is: high-pressure pump pressure-controlling 12-15MPa, inlet temperature: 155-185 ℃, temperature of outgoing air: 75-85 ℃;
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, and its condition is: high-pressure pump pressure-controlling 12-15MPa, inlet temperature: 155-185 ℃, temperature of outgoing air: 75-85 ℃;
10) with 6) described not hydrolytic residue pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying.
2. the method for extracting shinyleaf yellowhorn oil, Wood of Shinyleaf Yellowhorn polysaccharide and Wood of Shinyleaf Yellowhorn protolysate peptide from Wood of Shinyleaf Yellowhorn according to claim 1 is characterized in that: may further comprise the steps:
1) Wood of Shinyleaf Yellowhorn benevolence was pulverized 40 mesh sieves, the pure water that adds simultaneously Wood of Shinyleaf Yellowhorn benevolence weight 25% stirs, and places and makes its swelling in 1.5 hours;
2) the Wood of Shinyleaf Yellowhorn benevolence swelling thing of handling well is placed on the travelling belt and to carry out continuous microwave at moving process and process, the frequency of microwave is 2450MHz, and power is 60KW, and 110 ℃ of temperature, transfer rate are 3.0m/min, and the time is 2.0 min;
3) the Wood of Shinyleaf Yellowhorn benevolence after the microwave treatment is added 5.5 times of water of its weight, regulate pH value to 9, add 1.5% Sumizyme MP of Wood of Shinyleaf Yellowhorn benevolence weight, keep pH value 9 under 50 ℃ of conditions, be hydrolyzed 2.5 hours;
4) then at pH value 6.5-7.0, under 50 ℃ of conditions, each adds 0.4% neutral protease, flavor protease, cellulase, the hemicellulose enzymic hydrolysis 3 hours of Wood of Shinyleaf Yellowhorn benevolence weight;
5) with 4) the described liquid that obtains is warming up to 90 ℃, stirs 15min go out enzyme, breakdown of emulsion;
6) with 5) the described liquid that obtains enters the three-phase decanter whizzer by fresh feed pump and carries out three phase separation, obtains respectively oil phase, water and unhydrolysed residue solid phase;
7) with 6) the described aqueous phase solution that is mainly Wood of Shinyleaf Yellowhorn polysaccharide and protolysate peptide that obtains obtains the Wood of Shinyleaf Yellowhorn polysaccharide soln by the ultra-filtration membrane ultrafiltration and concentration of molecular weight 5000, and then removes residual sugar and be concentrated into protein peptide content at 15-20% by the nanofiltration of molecular weight 400;
8) with 7) the described Wood of Shinyleaf Yellowhorn polysaccharide concentrated solution that obtains carries out drying by the press spray moisture eliminator, and its condition is: high-pressure pump pressure-controlling 13MPa, inlet temperature: 170 ℃, temperature of outgoing air: 80 ℃;
9) with 7) the described protein polypeptide liquid that obtains carries out drying by the press spray moisture eliminator, and its condition is: high-pressure pump pressure-controlling 14MPa, inlet temperature: 170 ℃, temperature of outgoing air: 80 ℃;
10) with 6) described not hydrolytic residue pulverize to obtain to be rich in albumen, fat, cellulosic high-quality animal-feed by vacuum-drying.
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| CN110862862A (en) * | 2019-11-28 | 2020-03-06 | 内蒙古农业大学 | Method for simultaneously preparing xanthoceras sorbifolia Bunge oil and xanthoceras sorbifolia Bunge protein by aqueous enzymatic method |
| CN114250112A (en) * | 2021-11-15 | 2022-03-29 | 煌上煌集团有限公司 | Process for synchronously extracting polyphenol tea oil, tea-oil tree polypeptide and tea-oil tree polysaccharide |
| CN115029405B (en) * | 2022-04-04 | 2023-08-22 | 国药肽谷有限公司 | Method for extracting shinyleaf yellowhorn peptide |
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| WO2005037200A2 (en) * | 2003-10-09 | 2005-04-28 | Pacific Arrow Limited | Composition comprising xanthoceras sorbifolia extracts, compounds isolated from same, methods for preparing same and uses thereof |
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| WO2005037200A2 (en) * | 2003-10-09 | 2005-04-28 | Pacific Arrow Limited | Composition comprising xanthoceras sorbifolia extracts, compounds isolated from same, methods for preparing same and uses thereof |
| CN101695321A (en) * | 2009-10-23 | 2010-04-21 | 山东理工大学 | Method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction |
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