CN104921009A - Composite fruit peel extract having efficacies of lowering lipid and protecting livers - Google Patents
Composite fruit peel extract having efficacies of lowering lipid and protecting livers Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a composite fruit peel extract having the efficacies of lowering lipid and protecting livers. In accordance with the phenomenon that peels of massive fruits and vegetables are wasted in a conventional fruit and vegetable product processing process, water chestnut peel dregs, grape peel dregs, asparagus peels, and orange peels are used as raw materials, the raw materials are separately extracted, and then, the extracted raw materials are frozen, dried, crushed and mixed so as to prepare the composite fruit peel extract. The prepared composite fruit peel extract has better functions of lowering lipid and protecting livers, and is suitable for preparation of drugs or health products, which are used for preventing and treating fatty livers; besides, the composite fruit peel extract also has varied health-care efficacies of invigorating stomach for promoting digestion, resisting bacteria, resisting inflammation, and the like, so that the development prospects are broad.
Description
Technical field
The invention belongs to Medicines and Health Product technical field, be specifically related to a kind of composite fruit bark extract with lowering fat and protecting liver effect.
Background technology
Water chestnut (Eleocharis dulcis) has another name called water chestnut, Di Li, pure white, clear and melodious good to eat, the sweet succulence of taste of its meat, and has the effects such as clearing heat and detoxicating, cool blood promotes the production of body fluid, and both having can be used as fruit eats something rare, and can be used as again vegetables, is popular seasonal food.Existing cake, the drink with water chestnut being raw material is made deeply is liked by consumer, but can produce a large amount of water chestnut skin in water chestnut process in the market, and its quality accounts for the 20%-25% of fresh water chestnut quality, is abandoned and not only wastes resource, again contaminated environment.
Asparagus (Asparagus officinalis) is one of large famous dish in the world ten, and have another name called asparagus, it contains several amino acids, protein, vitamin, and content is all higher than general fruits and vegetables, has the good name of " kings of vegetables ".It is reported, nutritional labeling kind in asparagus peel is identical with the asparagus after peeling, and the content of part nutritional labeling is even higher than peeling asparagus, but in the process of processing asparagus canning product and quick frozen product, the asparagus peel accounting for raw material weight 15%-25% is all dropped, and causes great waste.
Fatty liver is that the liver fat venereal disease caused by many reasons becomes, and mainly because significant quantities of fat is piled up in liver cell, makes liver function damage cause liver fibrosis, even cirrhosis occurs.According to statistics, China's fatty liver adult prevalence rate is at 5%-9% in recent years, and has the trend of cumulative year after year.The present invention makes full use of the nutritive value of fruits and vegetables skin, and with water chestnut skin slag, asparagus peel slag, Grape Skin, orange peel for raw material, the composite fruit bark extract through extracting, being mixed has good lowering fat and protecting liver effect.
Summary of the invention
The object of the present invention is to provide a kind of composite fruit bark extract with lowering fat and protecting liver effect, its phenomenon be wasted for fruits and vegetables skin a large amount of in existing fruit and vegetable food process, prepares a kind of composite fruit bark extract with good lowering fat and protecting liver effect with water chestnut skin slag, asparagus peel slag, Grape Skin, orange peel for raw material.
For achieving the above object, the present invention adopts following technical scheme:
There is the composite fruit bark extract of lowering fat and protecting liver effect, be after water chestnut skin slag, grape skin, asparagus peel, orange peel are extracted respectively through freeze drying, pulverize, be mixed; Described component comprises by weight: water chestnut skin slag 10-15 part, grape skin 5-8 part, asparagus peel 5-8 part, orange peel 3-5 part.
The extracting method of described water chestnut skin slag is: by the water chestnut skin slag cleaning of reclaiming, dry rear coarse crushing, put into extraction kettle and carry out supercritical CO
2extraction, extraction kettle temperature is 40 ~ 80 DEG C, and pressure is 10 ~ 30MPa, extraction time 1 ~ 2h; Then extract is flowed into separating still and carry out the second-order separation, first order separating pressure 8 ~ 10MPa, separation temperature 50 ~ 80 DEG C, second level separating pressure 4 ~ 8MPa, separation temperature 40 ~ 60 DEG C; Gained parting liquid is evaporated to dry.
The extracting method of described grape skin is: the grape skin of recovery is added its weight 3-5 doubly, mass concentration is the ethanolic solution of 60%-70%, ultrasonic extraction 2-3 time at 30-50 DEG C, each 20-30min, filter, merging filtrate, filtrate reduced in volume, to medicinal extract, then adopts purification with macroreticular resin, collects eluent and is evaporated to dry.
The extracting method of described orange peel is: the orange peel reclaimed is cleaned, dries, pulverize after cross 60-80 mesh sieve, add orange peel weight 2-3 doubly, mass concentration is the Ca(OH of 20%-30%)
2the aqueous solution, and adjust pH is 9 ~ 12, refluxing extraction 2-3 time, each 1-2h, filters while hot, and filtrate under agitation adds hydrochloric acid and adjusts pH to be 2-5, and then continue to stir 40-80min, leave standstill cooling, suction filtration, filter cake distilled water is washed till neutrality, drains away the water.
The extracting method of described asparagus peel is: cross 60-80 mesh sieve by after the asparagus peel cleaning of reclaiming, oven dry, pulverizing, add asparagus peel weight 2-3 water doubly, 30-40min sterilizing is boiled in heating, then be cooled to room temperature, regulate pH value of solution to be 6.5, then add the mixed bacteria of liquor capacity 3%-5%, constant temperature culture 18-20h at 32-35 DEG C, through high speed centrifugation after taking-up, get precipitation distilled water and be washed till neutrality, for subsequent use.Mixed bacteria used is mixed by weight 1:2 bacillus subtilis and trichoderma viride.
The described composite fruit bark extract with lowering fat and protecting liver effect can be used for preparing lipotropic medicine or health products.
remarkable advantage of the present invention is:
1. the puchiin contained in water chestnut bark extract has good antibacterial action, also has certain effect to hypertension and hyperlipemia, anti-cancer; And the flavone compound such as water chestnut A prime, water chestnut B prime contained by it not only has liver protecting, protects the multiple efficacies such as cardiovascular, antibacterial, antitumor, also there is good effect to blood lipids index such as reduction serum total cholesterol, triglycerides, low-density lipoproteins.Containing the abundant functional component such as anthocyanin, resveratrol in grape skin extract, it can not only reduce human serum cholesterol levels, also can suppress triglycerides, the cholesterol deposition in liver, and can repair the liver cell damaged, the prevention and therapy for fatty liver has better effects.Containing abundant dietary fiber in asparagus peel, dietary fiber plays important role on maintenance body health, and pectin contained by it can in conjunction with cholesterol, lignin can conjucated bile acids, thus is played the effect of lipopenicillinase by the consumption of increase body inner cholesterol.The aurantiamarin contained in orange peel extract can reduce the required lipid of apolipoprotein synthesis, and suppresses microsomal trigalloyl transport protein active, also can reduce liver inner cholesterol.In addition, the present invention uses orange peel in the feed, can balance the cold and cool property of water chestnut, asparagus, makes gained composite fruit bark extract performance more perfect.
2. the present invention adopts the dietary fiber component in fermentation method extraction asparagus peel, and mix as zymocyte liquid using trichoderma viride and bacillus subtilis, utilize the active higher feature of trichoderma viride cellulase-producing on the one hand, the fiber in asparagus peel is degraded; But utilize the feature of bacillus subtilis self synthetic starch enzyme, neutral proteinase, lipase on the other hand, remove starch, albumen and fat in asparagus peel, namely reached the object of extraction, purifying by a step enzymolysis.
3. present invention achieves the recycling of fruits and vegetables skin discarded object in fruit and vegetable food process, not only can effectively reduce the wasting of resources and environmental pollution, also take full advantage of the nutritional labeling in fruits and vegetables skin, gained composite fruit bark extract is made to have good lowering fat and protecting liver effect, can be used for preparing lipotropic medicine or health products, for industry deep processing provides new direction.Meanwhile, gained composite fruit bark extract also has the various health care functions such as stomach strengthening and digestion promoting, antibacterial, anti-inflammatory, and DEVELOPMENT PROSPECT is wide.
Detailed description of the invention
More being convenient to make content of the present invention understand, below in conjunction with detailed description of the invention, technical solutions according to the invention are described further, but the present invention being not limited only to this.
Embodiment 1
A kind of preparation method with the composite fruit bark extract of lowering fat and protecting liver effect comprises the steps:
1) preparation of water chestnut bark extract: coarse crushing after the water chestnut skin slag cleaning of being reclaimed by 1.0 kg, oven dry, puts into extraction kettle and carries out supercritical CO
2extraction, extraction kettle temperature is 40 DEG C, and pressure is 30MPa, extraction time 1h; Then extract is flowed into separating still and carry out the second-order separation, first order separating pressure 8MPa, separation temperature 50 DEG C, second level separating pressure 4MPa, separation temperature 40 DEG C; Gained parting liquid is evaporated to dry;
2) preparation of grape skin extract: by 0.5 kg reclaim grape skin adds its weight 3 times, mass concentration is the ethanolic solution of 70%, ultrasonic extraction 3 times at 30 DEG C, each 20min, filter, merging filtrate, filtrate reduced in volume, to medicinal extract, then adopts purification with macroreticular resin, collects eluent and is evaporated to dry;
3) preparation of orange peel extract: the orange peel reclaimed by 0.3 kg is cleaned, dry, pulverizing is rear crosses 80 mesh sieves, adds orange peel weight 2 times, mass concentration is the Ca(OH of 30%)
2the aqueous solution, and adjust pH is 9, refluxing extraction 2 times, each 1h, filters while hot, and filtrate under agitation adds hydrochloric acid and adjusts pH to be 2, and then continue to stir 40min, leave standstill cooling, suction filtration, filter cake distilled water is washed till neutrality, drains away the water;
4) preparation of asparagus peel extract: cross 80 mesh sieves after the asparagus peel cleaning of being reclaimed by 0.5 kg, oven dry, pulverizing, add the water of asparagus peel weight 2 times, 40min sterilizing is boiled in heating, then be cooled to room temperature, regulate pH value of solution to be 6.5, then the mixed bacteria adding liquor capacity 3%, mixed by weight 1:2 by bacillus subtilis and trichoderma viride, constant temperature culture 20h at 32 DEG C, through high speed centrifugation after taking-up, get precipitation distilled water and be washed till neutrality, for subsequent use;
5) preparation of pericarp compound: the mixing after freeze drying, ultramicro grinding cross 200-300 mesh sieve of gained water chestnut bark extract, grape skin extract, asparagus peel extract, orange peel extract is obtained finished product.
Embodiment 2
A kind of preparation method with the composite fruit bark extract of lowering fat and protecting liver effect comprises the steps:
1) preparation of water chestnut bark extract: coarse crushing after the water chestnut skin slag cleaning of being reclaimed by 1.2 kg, oven dry, puts into extraction kettle and carries out supercritical CO
2extraction, extraction kettle temperature is 60 DEG C, and pressure is 20MPa, extraction time 1h; Then extract is flowed into separating still and carry out the second-order separation, first order separating pressure 9MPa, separation temperature 60 DEG C, second level separating pressure 6MPa, separation temperature 50 DEG C; Gained parting liquid is evaporated to dry;
2) preparation of grape skin extract: by 0.6 kg reclaim grape skin adds its weight 4 times, mass concentration is the ethanolic solution of 65%, ultrasonic extraction 2 times at 40 DEG C, each 30min, filter, merging filtrate, filtrate reduced in volume, to medicinal extract, then adopts purification with macroreticular resin, collects eluent and is evaporated to dry;
3) preparation of orange peel extract: the orange peel reclaimed by 0.4 kg is cleaned, dry, pulverizing is rear crosses 80 mesh sieves, adds orange peel weight 2 times, mass concentration is the Ca(OH of 25%)
2the aqueous solution, and adjust pH is 10, refluxing extraction 2 times, each 2h, filters while hot, and filtrate under agitation adds hydrochloric acid and adjusts pH to be 4, and then continue to stir 60min, leave standstill cooling, suction filtration, filter cake distilled water is washed till neutrality, drains away the water;
4) preparation of asparagus peel extract: cross 60 mesh sieves after the asparagus peel cleaning of being reclaimed by 0.6 kg, oven dry, pulverizing, add the water of asparagus peel weight 2 times, 35min sterilizing is boiled in heating, then be cooled to room temperature, regulate pH value of solution to be 6.5, then the mixed bacteria adding liquor capacity 4%, mixed by weight 1:2 by bacillus subtilis and trichoderma viride, constant temperature culture 20h at 34 DEG C, through high speed centrifugation after taking-up, get precipitation distilled water and be washed till neutrality, for subsequent use;
5) preparation of pericarp compound: the mixing after freeze drying, ultramicro grinding cross 200-300 mesh sieve of gained water chestnut bark extract, grape skin extract, asparagus peel extract, orange peel extract is obtained finished product.
Embodiment 3
A kind of preparation method with the composite fruit bark extract of lowering fat and protecting liver effect comprises the steps:
1) preparation of water chestnut bark extract: coarse crushing after the water chestnut skin slag cleaning of being reclaimed by 1.5 kg, oven dry, puts into extraction kettle and carries out supercritical CO
2extraction, extraction kettle temperature is 80 DEG C, and pressure is 10MPa, extraction time 2h; Then extract is flowed into separating still and carry out the second-order separation, first order separating pressure 10MPa, separation temperature 80 DEG C, second level separating pressure 8MPa, separation temperature 60 DEG C; Gained parting liquid is evaporated to dry;
2) preparation of grape skin extract: by 0.8 kg reclaim grape skin adds its weight 5 times, mass concentration is the ethanolic solution of 60%, ultrasonic extraction 2 times at 50 DEG C, each 20min, filter, merging filtrate, filtrate reduced in volume, to medicinal extract, then adopts purification with macroreticular resin, collects eluent and is evaporated to dry;
3) preparation of orange peel extract: the orange peel reclaimed by 0.5 kg is cleaned, dry, pulverizing is rear crosses 60 mesh sieves, adds orange peel weight 3 times, mass concentration is the Ca(OH of 20%)
2the aqueous solution, and adjust pH is 12, refluxing extraction 3 times, each 1h, filters while hot, and filtrate under agitation adds hydrochloric acid and adjusts pH to be 5, and then continue to stir 80min, leave standstill cooling, suction filtration, filter cake distilled water is washed till neutrality, drains away the water;
4) preparation of asparagus peel extract: cross 60 mesh sieves after the asparagus peel cleaning of being reclaimed by 0.8 kg, oven dry, pulverizing, add the water of asparagus peel weight 3 times, 30min sterilizing is boiled in heating, then be cooled to room temperature, regulate pH value of solution to be 6.5, then the mixed bacteria adding liquor capacity 5%, mixed by weight 1:2 by bacillus subtilis and trichoderma viride, constant temperature culture 18h at 35 DEG C, through high speed centrifugation after taking-up, get precipitation distilled water and be washed till neutrality, for subsequent use;
5) preparation of pericarp compound: the mixing after freeze drying, ultramicro grinding cross 200-300 mesh sieve of gained water chestnut bark extract, grape skin extract, asparagus peel extract, orange peel extract is obtained finished product.
Proved by zoopery, composite fruit bark extract provided by the present invention can make serum total cholesterol content reduce by more than 42%, and low-density lipoprotein content reduces by more than 38%, and hdl concentration raises more than 43%, and impaired liver cell can be repaired, there is good lowering fat and protecting liver effect.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (7)
1. there is the composite fruit bark extract of lowering fat and protecting liver effect, it is characterized in that: after water chestnut skin slag, grape skin, asparagus peel, orange peel are extracted respectively through freeze drying, pulverize, be mixed;
Described component comprises by weight: water chestnut skin slag 10-15 part, grape skin 5-8 part, asparagus peel 5-8 part, orange peel 3-5 part.
2. there is the preparation method of the composite fruit bark extract of lowering fat and protecting liver effect according to claim 1, it is characterized in that: the extracting method of described water chestnut skin slag is: by the water chestnut skin slag cleaning of reclaiming, dry rear coarse crushing, put into extraction kettle and carry out supercritical CO
2extraction, extraction kettle temperature is 40 ~ 80 DEG C, and pressure is 10 ~ 30MPa, extraction time 1 ~ 2h; Then extract is flowed into separating still and carry out the second-order separation, first order separating pressure 8 ~ 10MPa, separation temperature 50 ~ 80 DEG C, second level separating pressure 4 ~ 8MPa, separation temperature 40 ~ 60 DEG C; Gained parting liquid is evaporated to dry.
3. there is the preparation method of the composite fruit bark extract of lowering fat and protecting liver effect according to claim 1, it is characterized in that: the extracting method of described grape skin is: the grape skin of recovery is added its weight 3-5 doubly, mass concentration is the ethanolic solution of 60%-70%, ultrasonic extraction 2-3 time at 30-50 DEG C, each 20-30min, filter, merging filtrate, filtrate reduced in volume is to medicinal extract, then adopt purification with macroreticular resin, collect eluent and be evaporated to dry.
4. there is the preparation method of the composite fruit bark extract of lowering fat and protecting liver effect according to claim 1, it is characterized in that: the extracting method of described orange peel is: the orange peel reclaimed is cleaned, dries, pulverize after cross 60-80 mesh sieve, add orange peel weight 2-3 doubly, mass concentration is the Ca(OH of 20%-30%)
2the aqueous solution, and adjust pH is 9 ~ 12, refluxing extraction 2-3 time, each 1-2h, filters while hot, and filtrate under agitation adds hydrochloric acid and adjusts pH to be 2-5, and then continue to stir 40-80min, leave standstill cooling, suction filtration, filter cake distilled water is washed till neutrality, drains away the water.
5. there is the preparation method of the composite fruit bark extract of lowering fat and protecting liver effect according to claim 1, it is characterized in that: the extracting method of described asparagus peel is: cross 60-80 mesh sieve by after the asparagus peel cleaning of reclaiming, oven dry, pulverizing, add asparagus peel weight 2-3 water doubly, 30-40min sterilizing is boiled in heating, then room temperature is cooled to, pH value of solution is regulated to be 6.5, add the mixed bacteria of liquor capacity 3%-5% again, constant temperature culture 18-20h at 32-35 DEG C, through high speed centrifugation after taking-up, get precipitation distilled water and be washed till neutrality, for subsequent use.
6. there is the preparation method of the composite fruit bark extract of lowering fat and protecting liver effect according to claim 5, it is characterized in that: mixed bacteria used is mixed by weight 1:2 bacillus subtilis and trichoderma viride.
7. there is an application for the composite fruit bark extract of lowering fat and protecting liver effect as claimed in claim 1, it is characterized in that: described composite fruit bark extract is for the preparation of lipotropic medicine or health products.
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Application publication date: 20150923 |