CN104840587A - Method for extracting longan seed polyphenol - Google Patents

Method for extracting longan seed polyphenol Download PDF

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Publication number
CN104840587A
CN104840587A CN201510165362.0A CN201510165362A CN104840587A CN 104840587 A CN104840587 A CN 104840587A CN 201510165362 A CN201510165362 A CN 201510165362A CN 104840587 A CN104840587 A CN 104840587A
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China
Prior art keywords
seed
arillus longan
polyphenol
powder
extract
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CN201510165362.0A
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Chinese (zh)
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蓝擎
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Guangxi University
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Guangxi University
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Priority to CN201510165362.0A priority Critical patent/CN104840587A/en
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Abstract

The invention discloses a method for extracting longan seed polyphenol, and belongs to the field of natural product extraction method. The method comprises the following steps: 1) peeling and crushing; 2) filtering; 3) extracting; and 4) concentrating to obtain the polyphenol. The invention has the advantages of reasonable control of drying level of the longan seed, reduction of activity of polyphenol oxidase in longan seed, reduction of the loss of polyphenol in the production process, easiness to operate, high extraction efficiency and low production cost. The method has important meaning for expanding the use of longan seed and increasing their added value, not only is conducive to the full use of longan resources, but also helps promoting the development of longan planting.

Description

A kind of extraction seed of Arillus Longan polyphenol method
Technical field
The invention belongs to the extracting method field of natural product, particularly one extracts seed of Arillus Longan polyphenol method.
Background technology
Arillus Longan, for the fruit of sapindaceous plant Arillus Longan, sweet in the mouth warm in nature, heart-spleen boosting, fill blood, have and well have nourishing benefiting action, the dizzy disease China that waits of insomnia forgetfulness palpitation with fear that can be used for caused by the heart insufficiency of the spleen damage insufficiency of vital energy and blood is YEAST IN LONGAN PRODUCTION big country of the world, main product exceedes in ground cultivated areas such as Sichuan, Guangdong, Guangxi Fujian, Arillus Longan output more than ten thousand tons, annual value of production nearly hundred million yuan, the weight of seed of Arillus Longan of all occupying first place in the world accounts for longan fruit fresh weight, owing to not fully utilizing, annual discarded seed of Arillus Longan weight reaches hundreds of thousands ton
Seed of Arillus Longan contains the natural products such as abundant starch, reducing sugar, crude fibre, protein, pectin, polyphenol, simultaneously also containing nutrients such as the necessary aminoacid of several kinds of mineral elements, vitamin and human body, and polyphenol is wherein otherwise known as " the 7th class nutrient ", recently find again that polyphenol participates in cellular signal transduction pathways and can interact with cell receptor.In addition, in vegetable food and medicinal plants, the antibacterial activity of polyphenols to different microorganisms is also studied widely, in addition, polyphenol also has prophylaxis of hypertension, antithrombotic, anti-gastric-ulcer, the biological activitys such as antiviral and the function such as skin care and beauty treatment lot of experiments result show, plant polyphenol has non-oxidizability, anticancer, defying age, effect such as antibacterial grade, appropriate picked-up plant polyphenol can have the pathogenetic effect of prevention and prohibition disease, have as the Natural antioxidant and food, health product, the potentiality of cosmetic active raw material, there is huge Development volue and wide market prospect, and present stage extracts for seed of Arillus Longan polyphenol and purifying process research, have not yet to see the report of the process with actual production meaning.
Summary of the invention
The object of the invention is to: for above-mentioned Problems existing, the invention provides a kind of extraction seed of Arillus Longan polyphenol method, can improve the utilization rate of Arillus Longan, make full use of resource, can improve the income that people plant Arillus Longan, the present invention is simple to operate, and DNA purity is high.
Technical scheme of the present invention is:
A kind of extraction seed of Arillus Longan polyphenol method, comprises the following steps:
1), remove the peel, pulverize: select the seed of Arillus Longan without insect pest to clean, toast 25-30h, peeling under temperature is 30-70 DEG C of temperature, then put into pulverizer pulverizing and obtain seed of Arillus Longan powder, the granularity of seed of Arillus Longan powder is below 20 orders;
2), filter: to step 1) in seed of Arillus Longan powder add the alcoholic solution of seed of Arillus Longan powder 4-8 times of weight, reflux, extract, 4-7h at 40-80 DEG C, the lixiviating solution obtained is filtered, collects filtrate;
3), extract: by step 2) in the filtrate of gained add ethyl acetate and extract, collect ethyl acetate portion, silica gel column chromatography in extraction into ethyl acetate portion, polyphenol and silica gel adsorption, non-polyphenol component is removed; Then adopt petroleum ether-chloroform gradient elution, collect eluent;
4), concentrated acquisition: eluent is concentrated into solid content and reaches 70%, then drying, obtains seed of Arillus Longan polyphenol powder.
Preferably, described step 2) add the alcoholic solution of walnut powder 5 times of weight.
Preferably, the alcoholic solution adding seed of Arillus Longan powder described in reflux, extract, 5 hours at 50 DEG C.
Beneficial effect of the present invention is: the present invention's one extracts seed of Arillus Longan polyphenol method, the oven dry level of its advantage conservative control seed of Arillus Longan, reduce polyphenol oxidase activity in seed of Arillus Longan, reduce polyphenol loss in process of production, the method has easy to operate, extraction efficiency is high, the advantages such as production cost is low, the method, for the utilization expanding seed of Arillus Longan, improves its added value significant, not only be conducive to making full use of of seed of Arillus Longan resource, and be conducive to the development promoting Arillus Longan plant husbandry.
Detailed description of the invention
For concrete example, the invention will be further described below:
Embodiment 1
A kind of extraction seed of Arillus Longan polyphenol method, comprises the following steps:
1), remove the peel, pulverize: select the seed of Arillus Longan without insect pest to clean, toast 25h, peeling under temperature is 30 DEG C of temperature, then put into pulverizer pulverizing and obtain seed of Arillus Longan powder, the granularity of seed of Arillus Longan powder is 15 orders;
2), filter: to step 1) in seed of Arillus Longan powder add the alcoholic solution of seed of Arillus Longan powder 4 times of weight, reflux, extract, 4h at 50 DEG C, the lixiviating solution obtained is filtered, collects filtrate;
3), extract: by step 2) in the filtrate of gained add ethyl acetate and extract, collect ethyl acetate portion, silica gel column chromatography in extraction into ethyl acetate portion, polyphenol and silica gel adsorption, non-polyphenol component is removed; Then adopt petroleum ether-chloroform gradient elution, collect eluent;
4), concentrated acquisition: eluent is concentrated into solid content and reaches 70%, then drying, obtains seed of Arillus Longan polyphenol powder.
In the scheme that above-described embodiment 1 adopts, the oven dry level of its advantage conservative control seed of Arillus Longan, reduce polyphenol oxidase activity in seed of Arillus Longan, reduce polyphenol loss in process of production, the method has easy to operate, extraction efficiency is high, the advantages such as production cost is low, the method, for the utilization expanding seed of Arillus Longan, improves its added value significant, not only be conducive to making full use of of seed of Arillus Longan resource, and be conducive to the development promoting Arillus Longan plant husbandry.
Embodiment 2
A kind of extraction seed of Arillus Longan polyphenol method, comprises the following steps:
1), remove the peel, pulverize: select the seed of Arillus Longan without insect pest to clean, toast 27h, peeling under temperature is 60 DEG C of temperature, then put into pulverizer pulverizing and obtain seed of Arillus Longan powder, the granularity of seed of Arillus Longan powder is 20 orders;
2), filter: to step 1) in seed of Arillus Longan powder add the alcoholic solution of seed of Arillus Longan powder 6 times of weight, reflux, extract, 5h at 60 DEG C, the lixiviating solution obtained is filtered, collects filtrate;
3), extract: by step 2) in the filtrate of gained add ethyl acetate and extract, collect ethyl acetate portion, silica gel column chromatography in extraction into ethyl acetate portion, polyphenol and silica gel adsorption, non-polyphenol component is removed; Then adopt petroleum ether-chloroform gradient elution, collect eluent;
4), concentrated acquisition: eluent is concentrated into solid content and reaches 70%, then drying, obtains seed of Arillus Longan polyphenol powder.
In the scheme that above-described embodiment 2 adopts, the oven dry level of its advantage conservative control seed of Arillus Longan, reduce polyphenol oxidase activity in seed of Arillus Longan, reduce polyphenol loss in process of production, the method has easy to operate, extraction efficiency is high, the advantages such as production cost is low, the method, for the utilization expanding seed of Arillus Longan, improves its added value significant, not only be conducive to making full use of of seed of Arillus Longan resource, and be conducive to the development promoting Arillus Longan plant husbandry.
Embodiment 3
A kind of extraction seed of Arillus Longan polyphenol method, comprises the following steps:
1), remove the peel, pulverize: select the seed of Arillus Longan without insect pest to clean, toast 30h, peeling under temperature is 70 DEG C of temperature, then put into pulverizer pulverizing and obtain seed of Arillus Longan powder, the granularity of seed of Arillus Longan powder is 20 orders;
2), filter: to step 1) in seed of Arillus Longan powder add the alcoholic solution of seed of Arillus Longan powder 8 times of weight, reflux, extract, 7h at 80 DEG C, the lixiviating solution obtained is filtered, collects filtrate;
3), extract: by step 2) in the filtrate of gained add ethyl acetate and extract, collect ethyl acetate portion, silica gel column chromatography in extraction into ethyl acetate portion, polyphenol and silica gel adsorption, non-polyphenol component is removed; Then adopt petroleum ether-chloroform gradient elution, collect eluent;
4), concentrated acquisition: eluent is concentrated into solid content and reaches 70%, then drying, obtains seed of Arillus Longan polyphenol powder.
In the scheme that above-described embodiment 3 adopts, the oven dry level of its advantage conservative control seed of Arillus Longan, reduce polyphenol oxidase activity in seed of Arillus Longan, reduce polyphenol loss in process of production, the method has easy to operate, extraction efficiency is high, the advantages such as production cost is low, the method, for the utilization expanding seed of Arillus Longan, improves its added value significant, not only be conducive to making full use of of seed of Arillus Longan resource, and be conducive to the development promoting Arillus Longan plant husbandry.

Claims (3)

1. extract a seed of Arillus Longan polyphenol method, it is characterized in that, comprise the following steps:
1), remove the peel, pulverize: select the seed of Arillus Longan without insect pest to clean, toast 25-30h, peeling under temperature is 30-70 DEG C of temperature, then put into pulverizer pulverizing and obtain seed of Arillus Longan powder, the granularity of seed of Arillus Longan powder is below 20 orders;
2), filter: to step 1) in seed of Arillus Longan powder add the alcoholic solution of seed of Arillus Longan powder 4-8 times of weight, reflux, extract, 4-7h at 40-80 DEG C, the lixiviating solution obtained is filtered, collects filtrate;
3), extract: by step 2) in the filtrate of gained add ethyl acetate and extract, collect ethyl acetate portion, silica gel column chromatography in extraction into ethyl acetate portion, polyphenol and silica gel adsorption, non-polyphenol component is removed; Then adopt petroleum ether-chloroform gradient elution, collect eluent;
4), concentrated acquisition: eluent is concentrated into solid content and reaches 70%, then drying, obtains seed of Arillus Longan polyphenol powder.
2. one according to claim 1 extracts seed of Arillus Longan polyphenol method, it is characterized in that: described step 2) add the alcoholic solution of walnut powder 5 times of weight.
3. one according to claim 1 extracts seed of Arillus Longan polyphenol method, it is characterized in that: described in add seed of Arillus Longan powder alcoholic solution reflux, extract, 5 hours at 50 DEG C.
CN201510165362.0A 2015-04-02 2015-04-02 Method for extracting longan seed polyphenol Pending CN104840587A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105211787A (en) * 2015-11-13 2016-01-06 谢镜国 A kind of extraction process of longan seed polyphenol
CN105231351A (en) * 2015-11-13 2016-01-13 谢镜国 Method for extracting longan seed polyphenol
CN105348331A (en) * 2015-11-05 2016-02-24 广西大学 Extraction method of plant polyphenol
CN106235331A (en) * 2016-07-30 2016-12-21 南京正宽医药科技有限公司 A kind of extracting method of Arillus Longan
CN111449979A (en) * 2020-05-25 2020-07-28 江南大学 Longan seed polyphenol-pumpkin seed polypeptide composition and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011168510A (en) * 2010-02-17 2011-09-01 Lion Corp Composition for oral cavity
CN102920830A (en) * 2012-11-28 2013-02-13 华南师范大学 Method for effectively extracting polyphenol from Longan seed

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011168510A (en) * 2010-02-17 2011-09-01 Lion Corp Composition for oral cavity
CN102920830A (en) * 2012-11-28 2013-02-13 华南师范大学 Method for effectively extracting polyphenol from Longan seed

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105348331A (en) * 2015-11-05 2016-02-24 广西大学 Extraction method of plant polyphenol
CN105211787A (en) * 2015-11-13 2016-01-06 谢镜国 A kind of extraction process of longan seed polyphenol
CN105231351A (en) * 2015-11-13 2016-01-13 谢镜国 Method for extracting longan seed polyphenol
CN106235331A (en) * 2016-07-30 2016-12-21 南京正宽医药科技有限公司 A kind of extracting method of Arillus Longan
CN111449979A (en) * 2020-05-25 2020-07-28 江南大学 Longan seed polyphenol-pumpkin seed polypeptide composition and preparation method thereof

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Application publication date: 20150819