CN103992876A - Extraction method of passionflower seed oil - Google Patents
Extraction method of passionflower seed oil Download PDFInfo
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- CN103992876A CN103992876A CN201410238245.8A CN201410238245A CN103992876A CN 103992876 A CN103992876 A CN 103992876A CN 201410238245 A CN201410238245 A CN 201410238245A CN 103992876 A CN103992876 A CN 103992876A
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Abstract
The invention discloses an extraction method of passionflower seed oil from passionflowers. The extraction method comprises the following steps: raw material treatment; compound enzyme treatment; supercritical CO2 fluid extraction; and vacuum drying to obtain a finished product. Compared with the prior art for directly carrying out supercritical CO2 fluid extraction on a passionflower raw material, the extraction method disclosed by the invention has the advantage of high content.
Description
Technical field
The present invention relates to a kind of method of extracting passionflower seed oil from Herba Passiflorae Caeruleae, especially relate to a kind of employing supercritical CO
2abstraction technique extracts the method for passionflower seed oil from Herba Passiflorae Caeruleae seed.
Background technology
Herba Passiflorae Caeruleae (formal name used at school: Passiflora caerulea L.) be the good to eat fruit of a kind of fragrance, the good reputation that has " king of fruit juice ", for perennial evergreen is climbed bejuco, strain state is graceful, evergreen all the year round, blade profile is peculiar, and pattern is bright-coloured, smell fragrance, sweet fruit acid is good to eat, fruit fragrance, edible fruit flesh, sweet and sour taste.With rich flavor, fragrance is pleasant.Herba Passiflorae Caeruleae originates in Brazil, afterwards in South America, there is plantation South Africa, Southeast Asian countries, Australia and each department, the South Pacific.Because the fruit shape of Herba Passiflorae Caeruleae is like egg, fruit juice color and luster as egg yolk and have the various fruits such as pineapple, piscidia, banana, mango, grass poison, watermelon, smoked plum, sugarcane and peaches and plums local flavor, so there is again the title of Purple Granadilla, passion fruit.Herba Passiflorae Caeruleae whole body is all precious, and according to surveying and determination, Herba Passiflorae Caeruleae fruit contains and surpasses 135 kinds of above aromatoising substances, according to chemical analysis, measure, in passion fruit, vitamin A, B, C rich content, also contain phosphorus, calcium, potassium, iron and 17 seed amino acids, go through the compound of 5 kinds, and nutrition and pharmaceutical use are very high.It is whole, root, leaf can be used as medicine, and has anti-inflammatory, pain relieving, invigorates blood circulation and the effect such as keep fit, lipopenicillinase is hypotensive, and pericarp can be processed into feed.In dahlia fruit juice, there are 165 kinds of compounds, are suitable for being processed into fruit juice most, or be mixed into fruit juice blends with other fruit (as mango, pineapple, piscidia, mandarin orange orange and apple etc.) processing, can improve significantly mouthfeel and the fragrance of these fruit syrup; Dahlia fruit juice forms peat-reek, has skin maintenance, removes tired, anti-aging, antihypertensive curative effect, has very high nutritive value, economic worth and ornamental value.
Herba Passiflorae Caeruleae single fruit weight 30-50 gram, wherein seed accounts for the heavy 7.5-11.5% of fruit, in passionflower seed, oleaginousness reaches 21.7-29.2, the unsaturated fatty acid content of passionflower seed oil is up to more than 80%, wherein linoleic acid content is 67.8-74.3, and linolic acid is essential fatty acid, can treat essential aliphatic acid deficiency disease and cardiovascular disorder, reduce human serum cholesterol and blood pressure, its nutritive value and medical functions all obtain domestic and international medical circle and nutritionist's abundant affirmation.Yet at present in the industrial application of Herba Passiflorae Caeruleae, passionflower seed is usually used as waste material and is abandoned and lose, this is a kind of waste really.But along with going deep into of passionflower seed effect research, its value that keeps healthy, therapeutic value is subject to people's attention gradually, and the research of the extracting method of passionflower seed oil has also obtained people's attention.At present, the extracting method of passionflower seed oil has milling process, raw mill method, solvent-extraction process, microwave extraction method, supercritical extraction.If notification number is CN101874528A, title is a kind of method of extracting passionflower seed oil by cold pressing process, discloses a kind of method and technique that adopts extracting passionflower seed oil by cold pressing process, obtains respectively cold press crude oil and oil cake, oil yield 13-18 after squeezing.Notification number is CN1710043, and title is a kind of method of extracting passionflower seed oil from Herba Passiflorae Caeruleae seed, discloses a kind of CO of using
2the extracting method of supercritical extraction extracts oil from passionflower seed.These researchs have certain progressive meaning, but go back Shortcomings part, are not that oily extraction yield is low, be exactly extraction time long, extraction cost is higher.
Summary of the invention
A kind of method of extracting passionflower seed oil from Herba Passiflorae Caeruleae that the invention provides simple and reliable process, be convenient to production, efficiency is high.
The present invention is achieved through the following technical solutions:
An extracting method for passionflower seed oil, comprises the steps:
A. get dry passionflower seed and pulverize as 60-80 order fine powder, the water that adds passionflower seed gross weight 2-3 doubly to measure, is heated to 40-50 ℃ and maintains 10-15 minute, obtains seed slurry;
B. enzymolysis: by the seed slurry obtaining, regulate pH5-6, be placed in sealing and fermenting tank, add the prozyme of polygalacturonase, sucrase, pectin lyase to carry out enzymolysis, enzymolysis time is 1-2 hour, hydrolysis temperature is 43-45 ℃, and the weight ratio of polygalacturonase, sucrase, pectin lyase is 5:3:2, and prozyme add-on is 40-80mg/kg;
C. supercritical CO
2fluid extraction: the enzymolysis seed slurry that step b is obtained is placed in extraction kettle, with supercritical CO
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 50-60% carries out supercritical extraction as entrainment agent, and the quality of described paste seed slurry and the volume ratio of described extraction kettle are 1:4, and the volume of described ethanolic soln and the volume ratio of described extraction kettle are 1:2; Described extraction temperature is 50-55 ℃, and extracting pressure is 20-25Mpa, and extraction time is 1.5-2.0 hour;
D. collect the crude oil having obtained after having extracted, crude oil carries out vacuum-drying to water content again and is less than 2% under the condition of absolute pressure 4.0kPa, obtains passionflower seed oil.
The volume of ethanolic soln of the present invention and the volume unit of described extraction kettle are L, and described paste seed slurry mass unit is Kg.
Preferably 55 ℃ of described extraction temperature, the preferred 25Mpa of extracting pressure, extraction time preferably 1.5 hours.
Adopt method of the present invention to take Herba Passiflorae Caeruleae seed as raw material extraction passionflower seed oil, compared with prior art, advantage of the present invention:
1, the present invention adopts prozyme to process seed slurry, oil molecule in seed slurry can be discharged, be beneficial to the extraction of subsequent handling, and, the present invention adopts prozyme to process seed slurry, compares polygalacturonase, cellulase, the prozyme of polygalacturonase, sucrase, pectin lyase, better effects if, decomposes more complete.
2,, in method of the present invention, not directly to use passionflower seed slurry to carry out supercritical CO
2fluid extraction, but the passionflower seed of crossing by enzymolysis processing starches to carry out supercritical CO
2fluid extraction, compares microwave treatment seed slurry or autoclaving seed slurry, and enzymolysis processing seed slurry cost is lower, can also improve extraction yield, and extraction yield of the present invention is up to more than 60%, and prior art is generally in 20% left and right.
3, method extraction conditions of the present invention is gentle, can not only improve oily extraction yield, and the oleic acid valency obtaining is low, lighter color, and without aflatoxin contamination, no solvent residue, extraction time is shorter, and extraction cost is lower.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiment.
Embodiment 1:
An extracting method for passionflower seed oil, comprises the steps:
A. getting dry passionflower seed 100kg pulverizing is 60 order fine powders, and the water that adds passionflower seed gross weight 2-3 doubly to measure, is heated to 40 ℃ and maintains 12 minutes, obtains seed slurry;
B. enzymolysis: by the seed slurry obtaining, regulate pH5, be placed in sealing and fermenting tank, add the prozyme of polygalacturonase, sucrase, pectin lyase to carry out enzymolysis, enzymolysis time is 2 hours, hydrolysis temperature is 43-45 ℃, and the weight ratio of polygalacturonase, sucrase, pectin lyase is 5:3:2, and prozyme add-on is 80mg/kg;
C. supercritical CO
2fluid extraction: the enzymolysis seed slurry that step b is obtained is placed in extraction kettle, with supercritical CO
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 50% carries out supercritical extraction as entrainment agent, and the quality of described paste seed slurry and the volume ratio of described extraction kettle are 1:4, and the volume of described ethanolic soln and the volume ratio of described extraction kettle are 1:2; Described extraction temperature is 55 ℃, and extracting pressure is 25Mpa, and extraction time is 1.5 hours;
D. collect the crude oil having obtained after having extracted, crude oil carries out vacuum-drying to water content again and is less than 2% under the condition of absolute pressure 4.0kPa, obtains passionflower seed oil.
Embodiment 2:
An extracting method for passionflower seed oil, comprises the steps:
A. getting dry passionflower seed 100kg pulverizing is 80 order fine powders, and the water that adds passionflower seed gross weight 2-3 doubly to measure, is heated to 50 ℃ and maintains 15 minutes, obtains seed slurry;
B. enzymolysis: by the seed slurry obtaining, regulate pH6, be placed in sealing and fermenting tank, add the prozyme of polygalacturonase, sucrase, pectin lyase to carry out enzymolysis, enzymolysis time is 1 hour, hydrolysis temperature is 43-45 ℃, and the weight ratio of polygalacturonase, sucrase, pectin lyase is 5:3:2, and prozyme add-on is 60mg/kg;
C. supercritical CO
2fluid extraction: the enzymolysis seed slurry that step b is obtained is placed in extraction kettle, with supercritical CO
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 55% carries out supercritical extraction as entrainment agent, and the quality of described paste seed slurry and the volume ratio of described extraction kettle are 1:4, and the volume of described ethanolic soln and the volume ratio of described extraction kettle are 1:2; Described extraction temperature is 50 ℃, and extracting pressure is 20Mpa, and extraction time is 2.0 hours;
D. collect the crude oil having obtained after having extracted, crude oil carries out vacuum-drying to water content again and is less than 2% under the condition of absolute pressure 4.0kPa, obtains passionflower seed oil.
Embodiment 3:
An extracting method for passionflower seed oil, comprises the steps:
A. getting dry passionflower seed 100kg pulverizing is 60 order fine powders, and the water that adds passionflower seed gross weight 2-3 doubly to measure, is heated to 45 ℃ and maintains 10 minutes, obtains seed slurry;
B. enzymolysis: by the seed slurry obtaining, regulate pH5.5, be placed in sealing and fermenting tank, add the prozyme of polygalacturonase, sucrase, pectin lyase to carry out enzymolysis, enzymolysis time is 1.5 hours, hydrolysis temperature is 43-45 ℃, and the weight ratio of polygalacturonase, sucrase, pectin lyase is 5:3:2, and prozyme add-on is 40mg/kg;
C. supercritical CO
2fluid extraction: the enzymolysis seed slurry that step b is obtained is placed in extraction kettle, with supercritical CO
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 60% carries out supercritical extraction as entrainment agent, and the quality of described paste seed slurry and the volume ratio of described extraction kettle are 1:4, and the volume of described ethanolic soln and the volume ratio of described extraction kettle are 1:2; Described extraction temperature is 55 ℃, and extracting pressure is 25Mpa, and extraction time is 1.5 hours;
D. collect the crude oil having obtained after having extracted, crude oil carries out vacuum-drying to water content again and is less than 2% under the condition of absolute pressure 4.0kPa, obtains passionflower seed oil.
Claims (2)
1. an extracting method for passionflower seed oil, comprises the steps:
A. get dry passionflower seed and pulverize as 60-80 order fine powder, the water that adds passionflower seed gross weight 2-3 doubly to measure, is heated to 40-50 ℃ and maintains 10-15 minute, obtains seed slurry;
B. enzymolysis: by the seed slurry obtaining, regulate pH5-6, be placed in sealing and fermenting tank, add the prozyme of polygalacturonase, sucrase, pectin lyase to carry out enzymolysis, enzymolysis time is 1-2 hour, hydrolysis temperature is 43-45 ℃, and the weight ratio of polygalacturonase, sucrase, pectin lyase is 5:3:2, and prozyme add-on is 40-80mg/kg;
C. supercritical CO
2fluid extraction: the enzymolysis seed slurry that step b is obtained is placed in extraction kettle, with supercritical CO
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 50-60% carries out supercritical extraction as entrainment agent, and the quality of described paste seed slurry and the volume ratio of described extraction kettle are 1:4, and the volume of described ethanolic soln and the volume ratio of described extraction kettle are 1:2; Described extraction temperature is 50-55 ℃, and extracting pressure is 20-25Mpa, and extraction time is 1.5-2.0 hour;
D. collect the crude oil having obtained after having extracted, crude oil carries out vacuum-drying to water content again and is less than 2% under the condition of absolute pressure 4.0kPa, obtains passionflower seed oil.
2. the method for extracting passionflower seed oil from Herba Passiflorae Caeruleae according to claim 1, is characterized in that: described extraction temperature is 55 ℃, and extracting pressure is 25Mpa, and extraction time is 1.5 hours.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450185A (en) * | 2014-10-27 | 2015-03-25 | 茂名市德威圣科技有限责任公司 | Method for extracting and preparing passion fruit flavor |
| CN104531340A (en) * | 2014-11-27 | 2015-04-22 | 安徽有机良庄农业科技发展有限公司 | Field muskmelon seed oil extraction method |
| CN105602709A (en) * | 2016-02-18 | 2016-05-25 | 南宁市品迪生物工程有限公司 | Process for producing passion fruit seed oil |
| WO2017219101A1 (en) * | 2016-06-24 | 2017-12-28 | Universidade Estadual De Campinas - Unicamp | Process for sequentially extracting bioactive compounds from passion fruit bagasse and use of said bioactive compounds |
| CN108079357A (en) * | 2018-01-31 | 2018-05-29 | 袁承淼 | A kind of preparation method of air freshener |
| CN108852929A (en) * | 2018-07-10 | 2018-11-23 | 珀莱雅化妆品股份有限公司 | A kind of preparation method with the guava leaf extract of antiallergic |
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| CN1710043A (en) * | 2005-06-17 | 2005-12-21 | 长沙桑霖生物科技有限公司 | Method for extracting passiflora seed oil from passiflora seeds |
| CN102199481A (en) * | 2011-04-13 | 2011-09-28 | 北海田野食品有限公司 | Method for extracting passion flower seed oil by utilizing aqueous enzymatic method |
| CN103193751A (en) * | 2013-03-27 | 2013-07-10 | 吉首大学 | Method for continuously extracting active ingredients from grape seeds |
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| CN1710043A (en) * | 2005-06-17 | 2005-12-21 | 长沙桑霖生物科技有限公司 | Method for extracting passiflora seed oil from passiflora seeds |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450185A (en) * | 2014-10-27 | 2015-03-25 | 茂名市德威圣科技有限责任公司 | Method for extracting and preparing passion fruit flavor |
| CN104531340A (en) * | 2014-11-27 | 2015-04-22 | 安徽有机良庄农业科技发展有限公司 | Field muskmelon seed oil extraction method |
| CN105602709A (en) * | 2016-02-18 | 2016-05-25 | 南宁市品迪生物工程有限公司 | Process for producing passion fruit seed oil |
| WO2017219101A1 (en) * | 2016-06-24 | 2017-12-28 | Universidade Estadual De Campinas - Unicamp | Process for sequentially extracting bioactive compounds from passion fruit bagasse and use of said bioactive compounds |
| CN108079357A (en) * | 2018-01-31 | 2018-05-29 | 袁承淼 | A kind of preparation method of air freshener |
| CN108852929A (en) * | 2018-07-10 | 2018-11-23 | 珀莱雅化妆品股份有限公司 | A kind of preparation method with the guava leaf extract of antiallergic |
| CN108852929B (en) * | 2018-07-10 | 2021-05-04 | 珀莱雅化妆品股份有限公司 | Preparation method of guava leaf extract with anti-allergy effect |
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Application publication date: 20140820 |