CN104872758B - The preparation method of cicada fungus burdock oral liquid - Google Patents
The preparation method of cicada fungus burdock oral liquid Download PDFInfo
- Publication number
- CN104872758B CN104872758B CN201510271479.7A CN201510271479A CN104872758B CN 104872758 B CN104872758 B CN 104872758B CN 201510271479 A CN201510271479 A CN 201510271479A CN 104872758 B CN104872758 B CN 104872758B
- Authority
- CN
- China
- Prior art keywords
- liquid
- cicada fungus
- burdock
- culture
- cicada
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 121
- 241000931705 Cicada Species 0.000 title claims abstract description 91
- 241000233866 Fungi Species 0.000 title claims abstract description 79
- 235000003130 Arctium lappa Nutrition 0.000 title claims abstract description 63
- 235000008078 Arctium minus Nutrition 0.000 title claims abstract description 63
- -1 al Species 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 240000005528 Arctium lappa Species 0.000 title 1
- 241000208843 Arctium Species 0.000 claims abstract description 63
- 102000004190 Enzymes Human genes 0.000 claims abstract description 35
- 108090000790 Enzymes Proteins 0.000 claims abstract description 35
- 229940088598 enzyme Drugs 0.000 claims abstract description 35
- 235000015097 nutrients Nutrition 0.000 claims abstract description 35
- 239000002994 raw material Substances 0.000 claims abstract description 24
- 239000004382 Amylase Substances 0.000 claims abstract description 15
- 108010065511 Amylases Proteins 0.000 claims abstract description 15
- 102000013142 Amylases Human genes 0.000 claims abstract description 15
- 235000019418 amylase Nutrition 0.000 claims abstract description 15
- 108010059892 Cellulase Proteins 0.000 claims abstract description 14
- 229940106157 cellulase Drugs 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000012141 concentrate Substances 0.000 claims abstract description 12
- 238000011049 filling Methods 0.000 claims abstract description 11
- 238000003809 water extraction Methods 0.000 claims abstract description 9
- 238000000227 grinding Methods 0.000 claims abstract description 8
- 230000007062 hydrolysis Effects 0.000 claims abstract description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 6
- 239000001814 pectin Substances 0.000 claims abstract description 6
- 229920001277 pectin Polymers 0.000 claims abstract description 6
- 235000010987 pectin Nutrition 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims description 40
- 239000007787 solid Substances 0.000 claims description 27
- 239000000463 material Substances 0.000 claims description 26
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 23
- 239000011159 matrix material Substances 0.000 claims description 23
- 239000000243 solution Substances 0.000 claims description 23
- 239000002054 inoculum Substances 0.000 claims description 21
- 238000005286 illumination Methods 0.000 claims description 18
- 239000012528 membrane Substances 0.000 claims description 18
- 241000209140 Triticum Species 0.000 claims description 16
- 235000021307 Triticum Nutrition 0.000 claims description 16
- 239000011343 solid material Substances 0.000 claims description 16
- 235000021015 bananas Nutrition 0.000 claims description 14
- 235000019987 cider Nutrition 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 12
- 240000000588 Hericium erinaceus Species 0.000 claims description 9
- 235000007328 Hericium erinaceus Nutrition 0.000 claims description 9
- 239000000919 ceramic Substances 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 9
- 238000001223 reverse osmosis Methods 0.000 claims description 9
- 235000019991 rice wine Nutrition 0.000 claims description 9
- 244000241872 Lycium chinense Species 0.000 claims description 8
- 235000015468 Lycium chinense Nutrition 0.000 claims description 8
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 8
- 235000013399 edible fruits Nutrition 0.000 claims description 8
- 235000002722 Dioscorea batatas Nutrition 0.000 claims description 7
- 235000006536 Dioscorea esculenta Nutrition 0.000 claims description 7
- 240000001811 Dioscorea oppositifolia Species 0.000 claims description 7
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 claims description 7
- 235000021028 berry Nutrition 0.000 claims description 7
- 239000000084 colloidal system Substances 0.000 claims description 7
- 238000013329 compounding Methods 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 7
- 230000009849 deactivation Effects 0.000 claims description 7
- 230000001066 destructive effect Effects 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 230000003287 optical effect Effects 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 238000010298 pulverizing process Methods 0.000 claims description 7
- 238000011218 seed culture Methods 0.000 claims description 7
- 238000010792 warming Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000000872 buffer Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 claims description 6
- 238000012859 sterile filling Methods 0.000 claims description 5
- 244000126002 Ziziphus vulgaris Species 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 240000008790 Musa x paradisiaca Species 0.000 claims 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims 3
- 238000012549 training Methods 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- 238000011068 loading method Methods 0.000 claims 1
- 239000001509 sodium citrate Substances 0.000 claims 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 230000036541 health Effects 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 235000013376 functional food Nutrition 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 240000005561 Musa balbisiana Species 0.000 description 10
- 240000008866 Ziziphus nummularia Species 0.000 description 8
- 230000006870 function Effects 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 235000013361 beverage Nutrition 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- XOJVHLIYNSOZOO-SWOBOCGESA-N Arctiin Chemical compound C1=C(OC)C(OC)=CC=C1C[C@@H]1[C@@H](CC=2C=C(OC)C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)=CC=2)C(=O)OC1 XOJVHLIYNSOZOO-SWOBOCGESA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000005473 carotenes Nutrition 0.000 description 2
- 230000000055 hyoplipidemic effect Effects 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- 241001116389 Aloe Species 0.000 description 1
- 241000205585 Aquilegia canadensis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 244000037364 Cinnamomum aromaticum Species 0.000 description 1
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 1
- 241001625026 Cordyceps cicadae Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 235000013717 Houttuynia Nutrition 0.000 description 1
- 240000000691 Houttuynia cordata Species 0.000 description 1
- 235000002710 Ilex cornuta Nutrition 0.000 description 1
- 241001310146 Ilex cornuta Species 0.000 description 1
- 235000010254 Jasminum officinale Nutrition 0.000 description 1
- 240000005385 Jasminum sambac Species 0.000 description 1
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- 244000241838 Lycium barbarum Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- ZZIKIHCNFWXKDY-UHFFFAOYSA-N Myriocin Natural products CCCCCCC(=O)CCCCCCC=CCC(O)C(O)C(N)(CO)C(O)=O ZZIKIHCNFWXKDY-UHFFFAOYSA-N 0.000 description 1
- 235000010326 Osmanthus heterophyllus Nutrition 0.000 description 1
- 241000721451 Pectinophora gossypiella Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 240000002800 Sanicula europaea Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 241000194020 Streptococcus thermophilus Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 244000193174 agave Species 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000001857 anti-mycotic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000002543 antimycotic Substances 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 1
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- ZZIKIHCNFWXKDY-GNTQXERDSA-N myriocin Chemical compound CCCCCCC(=O)CCCCCC\C=C\C[C@@H](O)[C@H](O)[C@@](N)(CO)C(O)=O ZZIKIHCNFWXKDY-GNTQXERDSA-N 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000024053 secondary metabolic process Effects 0.000 description 1
- 235000008113 selfheal Nutrition 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
- A23L2/74—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Water Supply & Treatment (AREA)
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation method of cicada fungus burdock oral liquid, belong to plant nutrient ingredient extractive technique field.The preparation method of described cicada fungus burdock oral liquid comprises the following steps：(1) preparation of Periostracum cicadae liquid seeds；(2) cultivation of Periostracum cicadae；(3) hot water extraction, homogenate after coarse crushing；(4) wet method high shear ultramicro grinding；(5) cellulase, amylase, pectin enzyme hydrolysis；(6) filter, concentrate, be filling, obtain cicada fungus burdock oral liquid.Life cycle of the product of the present invention is short, raw material free of losses in process of producing product, no waste discharge in production process, is a kind of product output rate height, simple production process, green production method.In addition, cicada fungus burdock oral liquid health functional food of the present invention can be drunk for a long time as daily health caring, the product of relevant disease auxiliary treatment is also used as, has widened the application field of Periostracum cicadae and radix bardanae.
Description
Technical field
The invention belongs to plant nutrient ingredient extractive technique field, more particularly to a kind of preparation side of cicada fungus burdock oral liquid
Method.
Background technology
Periostracum cicadae is Clavicipitaceae Cordyceps sinensis fungus, also known as female cicada fungus, belongs to Fungi Imperfecti, is the conidium of big cicada grass
Stage is phorozoon period, is the fungi to form cordyceps sinensis cicada fungus, and China is for a long time remote as the history that medicinal material uses using medicinal fungus,
And Paecilomyces cicadae is then considered as one of " Chinese medicine eight delicacies ", early in Northern and Southern Dynasties' thunder school《Lei Gong Pao Zhi Lun》Just there is processing to record.Cicada is intended
Because containing the secondary metabolisms such as nucleosides material, myriocin, ergosterol, cordycepic acid, polysaccharide, hyaluronic acid and enzyme in mould
Product, have many bioactive functions for example immunological regulation, it is antitumor, regulation nervous system, improve renal function, protect liver, antibacterial,
It is antiviral etc..
The biennial herbaceous plant of Arctium composite family, alias burdock father-in-law dish, pizzle dish, bat thorn, Japan radish.It is it is a kind of with
The edible vegetables of loose fleshy root, its root delicacy delicious and crisp, petiole and tender leaf also edible.Burdock originates in China, 920 years Christian eras
Front and rear, burdock passes to Japan by China, and is cultivated into improved seeds, makes nutrition and health care and is worth a kind of high vegetable
Dish, fashionable Japan and South Korea, Southeast Asia of selling well, is described as " Fructus Arctii ".In recent years, China has introduced the new product of burdock from Japan
Kind, and planted on Beijing, Shanghai, Jiangsu, Shandong and other places rapidly, turn into the important agricultural exports in these areas, especially river
Su Xuzhou turns into the maximum burdock production base in China.Burdock has abundant nutritive value, rich in cellulose, carbohydrate, albumen
Matter, calcium, phosphorus, iron, alkaloid, vitamin B and needed by human 17 kinds of amino acid etc., its carotene carotene content occupies in vegetables
Two, the content of protein and calcium is first of tubers.The main value of burdock is more embodied in its medicinal and healthcare function, has strong
The function such as stomach QI invigorating, diuresis, nourishing and strengthening vital, clearing heat and detoxicating, antibacterial, anti-inflammatory, while have anti-curing oncoma, coronary heart disease, diabetes, knot
The function such as intestines disease and constipation.According to《Compendium of Materia Medica》Record, burdock is warm-natured sweet, " can lead to the twelve regular channels, wash the five internal organs bad odor ", " long
Clothes are made light of one's life by commiting suicide resistance to old ".China《Modern Chinese herbal medicine voluminous dictionary》、《Dictionary of medicinal plant》Pharmacological action Deng national authority pharmacopeia burdock
It is summarised as 3 aspects：First, it is improved effect that is immune, promoting growth；Second, have and suppress tumour growth, the work of nourishing liver kidney-nourishing
With；3rd, there are antibacterial and antimycotic effect.With reference to other raw materials with health role, burdock is succeeded in developing can be more
Comprehensive health drink is another direction of burdock deep processing research.Dong Wei extensively waits compound with burdock, red date as raw materials research
Burdock healthy drink, Song Hui etc. are using burdock as primary raw material, with cordate houttuynia, selfheal, mulberry leaf, chrysanthemum, radix glycyrrhizae, cassia seed, rose
Rare flower, Jasmine, honeysuckle, radix glycyrrhizae etc. are that auxiliary material studies health-care cold tea.Wei Dong etc. using fig jam and burdock Normal juice as raw material,
It is strain with lactobacillus bulgaricus and streptococcus thermophilus, develops lactacidase fermenting beverage, using aloe, burdock as primary raw material,
Asparagus juice burdock vinegar hypolipemic health-care beverage is developed, and the hypolipemic function of the beverage is studied.
At present, the research respectively to Periostracum cicadae and radix bardanae is more, but by the research of the two comprehensive exploitation there is not yet
Report, not seen in the research for the health food that cicada fungus is succeeded in developing to energy property with reference to burdock.
The content of the invention
It is an object of the invention to provide a kind of preparation method of cicada fungus burdock oral liquid, it is intended to solves the production of Periostracum cicadae
Product form is dull, the problem of lacking cicada fungus burdock oral liquid.
The cicada fungus burdock oral liquid obtained another object of the present invention is to provide above-mentioned preparation method.
The purpose of the present invention is achieved through the following technical solutions：A kind of preparation method of cicada fungus burdock oral liquid, including with
Lower step：
(1) preparation of Periostracum cicadae liquid seeds；
(2) cultivation of Periostracum cicadae；
(3) hot water extraction, homogenate after coarse crushing；
(4) wet method high shear ultramicro grinding；
(5) cellulase, amylase, pectin enzyme hydrolysis；
(6) filter, concentrate, be filling, obtain cicada fungus burdock oral liquid.
As a preferred embodiment of the present invention, the preparation of the Periostracum cicadae liquid seeds described in step (1), specific steps
It is as follows：Periostracum cicadae culture liquid seed culture medium, using 10% cider, 10% bananas juice, 10% murphy juice according to 1:1:5
Volume ratio three kinds of liquid are mixed into inoculated and cultured after compounding, sterilizing, fluid nutrient medium loads 500ml triangular flasks, per triangular flask
Liquid amount is 200ml, and sterilize 15min~20min in 121 DEG C, accesses cicada fungus conidia powder, inoculum concentration 0.05g conidia powders/200ml
Fluid nutrient medium, 19 DEG C of 3~5d of isothermal vibration incubator culture.
As a preferred embodiment of the present invention, the cultivation of the Periostracum cicadae described in step (2), comprise the following steps that：Cicada
The solid culture of pink bollworm grass uses wheat berry and dry cicada powder as solid material, and the two is 50 according to quality:After 1 ratio mixes, put
Enter in cordyceps sinensis cultivation tray, add nutrient solution, per 1kg solid materials in nutrient solution addition be 1.1L, 121 DEG C of 20min that sterilize~
30min, inoculated and cultured after cooling；Inoculum concentration is：The inoculum concentration of Periostracum cicadae liquid seeds is 50ml in every kilogram of solid material, Gu
After the inoculation of body culture medium top layer, 20 DEG C of constant temperature illumination box 3~5d of light culture, treat that mycelia covers with top layer, you can see optical culture,
Light intensity is 1000Lux, daily illumination 12h；Illumination cultivation 20d, solid culture matrix grow a large amount of cicada fungus coremiums and start to produce
Spore, collect cicada fungus coremium, conidia powder, culture matrix.
Dry cicada powder described in step (2) obtains for cicada larva crushed after being dried.
Nutrient solution described in step (2) is 5% cider and 5% bananas juice volume ratio 1:4 mixing obtain.
As a preferred embodiment of the present invention, hot water extraction, homogenate, specific steps after the coarse crushing described in step (3)
It is as follows：By fresh 2~8 parts of cicada fungus, 10~15 parts of fresh burdock root, 2~8 parts of stir-baked RHIZOMA POLYGONATI with yellow rice wine, 3~5 parts of jujube, 1~5 part of Hericium erinaceus, Chinese holly
1~5 part of matrimony vine, 1~4 part of Chinese yam, the ratio of 1~4 part of light wheat mix raw material, using pulverizer comminuting matter, after crushing
The granularity of material reaches 80~100 mesh.
Fresh cicada fungus described in step (3) is conidia powder, coremium, culture matrix mass ratio are 1:5:15.
Jujube described in step (3) is stoning jujube.
As a preferred embodiment of the present invention, the wet method high shear ultramicro grinding described in step (4), concretely comprise the following steps：
Raw material after crushing is placed in reactor, using solid-liquid ratio as 1:20~30 ratio adds pure water, is cleared up in intelligent microwave in instrument,
Control pressure 0.3MPa, 80W~90W microwave treatment 80s~120s, the feed liquid after extracted is using high speed homogenizer in 5000r/
Homogenized 10min under the conditions of min.
As a preferred embodiment of the present invention, cellulase, amylase described in step (5), pectin enzyme hydrolysis, specifically
Step is：Feed liquid after homogenate makes Solids Particle Diameter in feed liquid be less than 1 through the further micronized pulverization of two-stage shear-type colloid mill
μm, in the range of being in 5~7 using the material liquid pH value after citric acid-sodium citrate buffer regulation homogenate, add enzyme and carry out
Enzymolysis, enzyme used in enzymolysis are 38~100U/g of cellulase, 500~800U/g of amylase, 50~80U/g of pectase, in 40~
To material 1~2h of vibrating at constant temperature for enzymolysis under 50 DEG C of temperature conditionss, after enzymolysis, the enzymolysis liquid of acquisition is warming up to 80 DEG C and is incubated
10min is with the activity of destructive enzyme.
It is filtering, concentration described in step (6), filling as a preferred embodiment of the present invention, concretely comprise the following steps：Enzyme deactivation
After feed liquid afterwards is using 0.45um ceramic membrane filter clarifying treatment, after reverse osmosis membrane concentrates, sterile filling, rufous is produced
Cicada fungus burdock oral liquid.
As a preferred embodiment of the present invention, a kind of preparation method of cicada fungus burdock oral liquid of the invention, specific system
Standby step is as follows：
(1) Periostracum cicadae culture liquid seed culture medium, pressed using 10% cider, 10% bananas juice, 10% murphy juice
According to 1:1:Inoculated and cultured after three kinds of liquid are mixed compounding, sterilized by 5 volume ratio, fluid nutrient medium load 500ml triangular flasks, often
Triangular flask liquid amount is 200ml, and sterilize 15min~20min in 121 DEG C, access cicada fungus conidia powder, inoculum concentration 0.05g conidia powders/
200ml fluid nutrient mediums, 19 DEG C of 3~5d of isothermal vibration incubator culture；
(2) solid culture of Periostracum cicadae uses wheat berry and dry cicada powder, and the two is 50 according to quality:1 ratio mixes
Afterwards, insert in cordyceps sinensis cultivation tray, add nutrient solution, be 1.1L per 1kg solid material nutrient solutions addition, 121 DEG C of 20min that sterilize~
30min, inoculated and cultured after cooling；Inoculum concentration is：Every kilogram of solid material inoculum concentration is 50ml, after the inoculation of solid medium top layer,
20 DEG C of constant temperature illumination box 3~5d of light culture, treat that mycelia covers with top layer, you can see optical culture, light intensity 1000Lux, daily
Illumination 12h；Illumination cultivation 20d, solid culture matrix grow a large amount of cicada fungus coremiums and start to produce spore, collect cicada fungus falx
Beam, conidia powder, culture matrix；Described nutrient solution is 5% cider and 5% bananas juice volume ratio 1:4 mixing obtain；
(3) by fresh 2~8 parts of cicada fungus, 10~15 parts of fresh burdock root, 2~8 parts of stir-baked RHIZOMA POLYGONATI with yellow rice wine, 3~5 parts of jujube, Hericium erinaceus 1~5
Part, 1~5 part of the fruit of Chinese wolfberry, 1~4 part of Chinese yam, the ratio of 1~4 part of light wheat mix raw material, use pulverizer comminuting matter, powder
The granularity of material reaches 80~100 mesh after broken；Described fresh cicada fungus is conidia powder, coremium, culture matrix mass ratio are 1:5:
15；
(4) raw material after crushing is placed in reactor, using solid-liquid ratio as 1:20~30 ratio adds pure water, micro- in intelligence
Ripple is cleared up in instrument, control pressure 0.3MPa, 80W~90W microwave treatment 80s~120s, and the feed liquid after extracted is even using high speed
Starch device homogenized 10min under the conditions of 5000r/min；
(5) feed liquid after being homogenized makes Solids Particle Diameter in feed liquid through the further micronized pulverization of two-stage shear-type colloid mill
Less than 1 μm, in the range of being in 5~7 using the material liquid pH value after citric acid-sodium citrate buffer regulation homogenate, add
Enzyme is digested, and enzyme used in enzymolysis is 38~100U/g of cellulase, 500~800U/g of amylase, 50~80U/g of pectase,
To material 1~2h of vibrating at constant temperature for enzymolysis under 40~50 DEG C of temperature conditionss, after enzymolysis, the enzymolysis liquid of acquisition is warming up to 80 DEG C
And 10min is incubated with the activity of destructive enzyme；
(6) after the feed liquid after enzyme deactivation is using 0.45um ceramic membrane filter clarifying treatment, after reverse osmosis membrane concentrates, sterile
It is filling, produce the cicada fungus burdock oral liquid of rufous.
A kind of cicada fungus burdock oral liquid is obtained by above-mentioned preparation method.
The present invention is had the following advantages relative to prior art and effect：
The invention discloses a kind of preparation method of cicada fungus burdock oral liquid, comprise the following steps:The culture of Periostracum cicadae,
It is sturdy with reference to commercially available fresh individual and without disease pest by cultured Periostracum cicadae coremium, culture matrix and caused conidia powder
The raw materials such as harmful radix bardanae, stir-baked RHIZOMA POLYGONATI with yellow rice wine, jujube, Hericium erinaceus, the fruit of Chinese wolfberry, Chinese yam, light wheat；After coarse crushing carry out hot water extraction,
Homogenate；It is hydrolyzed after soybean dietary fiber using cellulase, amylase and pectase, hydrolyzate is further across ceramic membrane
Filtering, filtered solution are condensed into a certain proportion of rufous transparency liquid through reverse osmosis membrane, and sterile filling can obtain cicada fungus burdock
Oral liquid.Life cycle of the product of the present invention is short, raw material free of losses in process of producing product, no waste discharge in production process,
It is a kind of product output rate height, simple production process, green production method.In addition, cicada fungus burdock oral liquid of the present invention
Health functional food can be drunk for a long time as daily health caring, be also used as the product of relevant disease auxiliary treatment, widened
The application field of Periostracum cicadae and radix bardanae, the cicada fungus burdock oral liquid of production contain it is a variety of including adenosine, it is cordycepin, more
Multiple biological activities composition including sugar, sterol, carrotene, polyphenol, amino acid, calcium, iron etc., have significantly strong kidney benefit spleen,
The biological function of stomach is protected in nourishing the liver.In addition, simple production process of the present invention, is advantageous to meet that consumer drinks pocket feature
The consumption demand of material.
Brief description of the drawings
Fig. 1 is a kind of flow chart of cicada fungus burdock oral liquid provided in an embodiment of the present invention.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are unlimited
In this.
Fig. 1 shows a kind of preparation method flow of cicada fungus burdock oral liquid provided by the invention.For convenience of description, only
It illustrate only part related to the present invention.
The invention provides a kind of preparation method of cicada fungus burdock oral liquid, comprise the following steps：
The preparation of S101 Periostracum cicadae liquid seeds；
The cultivation of S102 Periostracum cicadaes；
Hot water extraction, homogenate after S103 coarse crushings；
S104 wet method high shear ultramicro grindings；
S105 cellulases, amylase, pectin enzyme hydrolysis；
S106 filterings, concentrate, be filling.
The invention discloses a kind of preparation method of cicada fungus burdock oral liquid, by the culture of Periostracum cicadae, will cultivate
Periostracum cicadae coremium, culture matrix and caused conidia powder, sturdy and no disease and pests harm the burdock with reference to commercially available fresh individual
The raw materials such as root, stir-baked RHIZOMA POLYGONATI with yellow rice wine, jujube, Hericium erinaceus, the fruit of Chinese wolfberry, Chinese yam, light wheat；Hot water extraction, homogenate are carried out after coarse crushing；Wet method
It is hydrolyzed after ultramicro grinding using cellulase, amylase and pectase, hydrolyzate is further across ceramic membrane filter, filtration
Liquid is condensed into a certain proportion of rufous transparency liquid through reverse osmosis membrane, and sterile filling can obtain cicada fungus burdock oral liquid, wrap
Include hot water extraction, homogenate → wet method high shear ultramicro grinding → enzymolysis after cicada fungus liquid seeds preparation → cicada fungus cultivation → coarse crushing
→ filtering, concentrate, be filling.
Embodiment 1
A kind of preparation of cicada fungus burdock oral liquid of the present invention concretely comprises the following steps：
(1) Periostracum cicadae culture liquid seed culture medium, pressed using 10% cider, 10% bananas juice, 10% murphy juice
According to 1:1:Inoculated and cultured after three kinds of liquid are mixed compounding, sterilized by 5 volume ratio, fluid nutrient medium load 500ml triangular flasks, often
Triangular flask liquid amount is 200ml, and sterilize 15min in 121 DEG C, accesses cicada fungus conidia powder, inoculum concentration 0.05g conidia powders/200ml liquid
Body culture medium, 19 DEG C of isothermal vibration incubator culture 3d；
(2) solid culture of Periostracum cicadae uses wheat berry and dry cicada powder, and the two is 50 according to quality:1 ratio mixes
Afterwards, insert in cordyceps sinensis cultivation tray, add nutrient solution, be 1.1L per 1kg solid material nutrient solutions addition, 121 DEG C of 20min that sterilize,
Inoculated and cultured after cooling；Inoculum concentration is：Every kilogram of solid material inoculum concentration is 50ml, after the inoculation of solid medium top layer, 20 DEG C of perseverances
Warm illumination box light culture 3d, treats that mycelia covers with top layer, you can sees optical culture, light intensity 1000Lux, daily illumination 12h；Light
According to culture 20d, solid culture matrix grows a large amount of cicada fungus coremiums and starts to produce spore, collect cicada fungus coremium, conidia powder,
Culture matrix；Described nutrient solution is 5% cider and 5% bananas juice volume ratio 1:4 mixing obtain；
(3) by fresh 2 parts of cicada fungus, 10 parts of fresh burdock root, 2 parts of stir-baked RHIZOMA POLYGONATI with yellow rice wine, 3 parts of jujube, 1~5 part of Hericium erinaceus, 1 part of the fruit of Chinese wolfberry,
1 part of Chinese yam, the ratio of 1 part of light wheat mix raw material, and using pulverizer comminuting matter, the granularity of crushing rear material reaches 80
Mesh；Described fresh cicada fungus is conidia powder, coremium, culture matrix mass ratio are 1:5:15；
(4) raw material after crushing is placed in reactor, using solid-liquid ratio as 1:20~30 ratio adds pure water, micro- in intelligence
Ripple is cleared up in instrument, control pressure 0.3MPa, 80W microwave treatment 80s, and the feed liquid after extracted is using high speed homogenizer in 5000r/
Homogenized 10min under the conditions of min；
(5) feed liquid after being homogenized makes Solids Particle Diameter in feed liquid through the further micronized pulverization of two-stage shear-type colloid mill
Less than 1 μm, in the range of being in 5 using the material liquid pH value after citric acid-sodium citrate buffer regulation homogenate, add enzyme and enter
Row enzymolysis, enzyme used in enzymolysis is cellulase 38U/g, amylase 500U/g, pectase 50U/g, under 40 DEG C of temperature conditionss
To material vibrating at constant temperature for enzymolysis 1h, after enzymolysis, the enzymolysis liquid of acquisition is warming up to 80 DEG C and is incubated work of the 10min with destructive enzyme
Property；
(6) after the feed liquid after enzyme deactivation is using 0.45um ceramic membrane filter clarifying treatment, after reverse osmosis membrane concentrates, sterile
It is filling, produce the cicada fungus burdock oral liquid of rufous.
Embodiment 2
A kind of preparation of cicada fungus burdock oral liquid of the present invention concretely comprises the following steps：
(1) Periostracum cicadae culture liquid seed culture medium, pressed using 10% cider, 10% bananas juice, 10% murphy juice
According to 1:1:Inoculated and cultured after three kinds of liquid are mixed compounding, sterilized by 5 volume ratio, fluid nutrient medium load 500ml triangular flasks, often
Triangular flask liquid amount is 200ml, and sterilize 15min~20min in 121 DEG C, access cicada fungus conidia powder, inoculum concentration 0.05g conidia powders/
200ml fluid nutrient mediums, 19 DEG C of isothermal vibration incubator culture 5d；
(2) solid culture of Periostracum cicadae uses wheat berry and dry cicada powder, and the two is 50 according to quality:1 ratio mixes
Afterwards, insert in cordyceps sinensis cultivation tray, add nutrient solution, be 1.1L per 1kg solid material nutrient solutions addition, 121 DEG C of 30min that sterilize,
Inoculated and cultured after cooling；Inoculum concentration is：Every kilogram of solid material inoculum concentration is 50ml, after the inoculation of solid medium top layer, 20 DEG C of perseverances
Warm illumination box light culture 5d, treats that mycelia covers with top layer, you can sees optical culture, light intensity 1000Lux, daily illumination 12h；Light
According to culture 20d, solid culture matrix grows a large amount of cicada fungus coremiums and starts to produce spore, collect cicada fungus coremium, conidia powder,
Culture matrix；Described nutrient solution is 5% cider and 5% bananas juice volume ratio 1:4 mixing obtain；
(3) by fresh 5 parts of cicada fungus, 15 parts of fresh burdock root, 2 parts of stir-baked RHIZOMA POLYGONATI with yellow rice wine, 3 parts of jujube, 1 part of Hericium erinaceus, 1 part of the fruit of Chinese wolfberry, mountain
2 parts of medicine, the ratio of 1 part of light wheat mix raw material, and using pulverizer comminuting matter, the granularity of crushing rear material reaches 80 mesh；
Described fresh cicada fungus is conidia powder, coremium, culture matrix mass ratio are 1:5:15；
(4) raw material after crushing is placed in reactor, using solid-liquid ratio as 1:20 ratio adds pure water, disappears in intelligent microwave
Xie Yizhong, control pressure 0.3MPa, 80W microwave treatment 80s, the feed liquid after extracted is using high speed homogenizer in 5000r/min
Under the conditions of homogenized 10min；
(5) feed liquid after being homogenized makes Solids Particle Diameter in feed liquid through the further micronized pulverization of two-stage shear-type colloid mill
Less than 1 μm, in the range of being in 5~7 using the material liquid pH value after citric acid-sodium citrate buffer regulation homogenate, add
Enzyme is digested, and enzyme used in enzymolysis is cellulase 38U/g, amylase 500U/g, pectase 50U/g, in 50 DEG C of temperature strip
To material vibrating at constant temperature for enzymolysis 1h under part, after enzymolysis, the enzymolysis liquid of acquisition is warming up to 80 DEG C and is incubated 10min with destructive enzyme
Activity；
(6) after the feed liquid after enzyme deactivation is using 0.45um ceramic membrane filter clarifying treatment, after reverse osmosis membrane concentrates, sterile
It is filling, produce the cicada fungus burdock oral liquid of rufous.
Embodiment 3
A kind of preparation of cicada fungus burdock oral liquid of the present invention concretely comprises the following steps：
(1) Periostracum cicadae culture liquid seed culture medium, pressed using 10% cider, 10% bananas juice, 10% murphy juice
According to 1:1:Inoculated and cultured after three kinds of liquid are mixed compounding, sterilized by 5 volume ratio, fluid nutrient medium load 500ml triangular flasks, often
Triangular flask liquid amount is 200ml, and sterilize 20min in 121 DEG C, accesses cicada fungus conidia powder, inoculum concentration 0.05g conidia powders/200ml liquid
Body culture medium, 19 DEG C of isothermal vibration incubator culture 5d；
(2) solid culture of Periostracum cicadae uses wheat berry and dry cicada powder, and the two is 50 according to quality:1 ratio mixes
Afterwards, insert in cordyceps sinensis cultivation tray, add nutrient solution, be 1.1L per 1kg solid material nutrient solutions addition, 121 DEG C of 30min that sterilize,
Inoculated and cultured after cooling；Inoculum concentration is：Every kilogram of solid material inoculum concentration is 50ml, after the inoculation of solid medium top layer, 20 DEG C of perseverances
Warm illumination box light culture 5d, treats that mycelia covers with top layer, you can sees optical culture, light intensity 1000Lux, daily illumination 12h；Light
According to culture 20d, solid culture matrix grows a large amount of cicada fungus coremiums and starts to produce spore, collect cicada fungus coremium, conidia powder,
Culture matrix；Described nutrient solution is 5% cider and 5% bananas juice volume ratio 1:4 mixing obtain；
(3) by fresh 8 parts of cicada fungus, 15 parts of fresh burdock root, 8 parts of stir-baked RHIZOMA POLYGONATI with yellow rice wine, 5 parts of jujube, 1 part of Hericium erinaceus, 5 parts of the fruit of Chinese wolfberry, mountain
4 parts of medicine, the ratio of 4 parts of light wheat mix raw material, and using pulverizer comminuting matter, the granularity of crushing rear material reaches 100 mesh；
Described fresh cicada fungus is conidia powder, coremium, culture matrix mass ratio are 1:5:15；
(4) raw material after crushing is placed in reactor, using solid-liquid ratio as 1:30 ratio adds pure water, disappears in intelligent microwave
Xie Yizhong, control pressure 0.3MPa, 90W microwave treatment 120s, the feed liquid after extracted is using high speed homogenizer in 5000r/min
Under the conditions of homogenized 10min；
(5) feed liquid after being homogenized makes Solids Particle Diameter in feed liquid through the further micronized pulverization of two-stage shear-type colloid mill
Less than 1 μm, in the range of being in 7 using the material liquid pH value after citric acid-sodium citrate buffer regulation homogenate, add enzyme and enter
Row enzymolysis, enzyme used in enzymolysis is cellulase 100U/g, amylase 8 00U/g, pectase 80U/g, under 50 DEG C of temperature conditionss
To material vibrating at constant temperature for enzymolysis 2h, after enzymolysis, the enzymolysis liquid of acquisition is warming up to 80 DEG C and is incubated work of the 10min with destructive enzyme
Property；
(6) after the feed liquid after enzyme deactivation is using 0.45um ceramic membrane filter clarifying treatment, after reverse osmosis membrane concentrates, sterile
It is filling, produce the cicada fungus burdock oral liquid of rufous.
Life cycle of the product of the present invention is short, raw material free of losses in process of producing product, no waste discharge in production process,
It is a kind of product output rate height, simple production process, green production method.Product of the present invention is oral as cicada fungus burdock
Liquid acts not only as beverage and directly drunk, and is also used as the product of relevant disease auxiliary treatment, widened Periostracum cicadae and
The application field of radix bardanae.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (2)
- A kind of 1. preparation method of cicada fungus burdock oral liquid, it is characterised in that：Comprise the following steps：(1) preparation of Periostracum cicadae liquid seeds；(2) cultivation of Periostracum cicadae；(3) hot water extraction, homogenate after coarse crushing；(4) wet method high shear ultramicro grinding；(5) cellulase, amylase, pectin enzyme hydrolysis；(6) filter, concentrate, be filling, obtain cicada fungus burdock oral liquid；The preparation of Periostracum cicadae liquid seeds described in step (1), is comprised the following steps that：Periostracum cicadae culture liquid seed culture Base, using 10% cider, 10% bananas juice, 10% murphy juice according to 1:1:Three kinds of liquid are mixed compounding, gone out by 5 volume ratio Inoculated and cultured after bacterium, fluid nutrient medium load 500ml triangular flasks, are 200ml per triangular flask liquid amount, and sterilize 15min in 121 DEG C ~20min, access cicada fungus conidia powder, inoculum concentration 0.05g conidia powders/200ml fluid nutrient mediums, 19 DEG C of isothermal vibration incubator trainings Support 3~5d；The cultivation of Periostracum cicadae described in step (2), is comprised the following steps that：The solid culture of Periostracum cicadae is using wheat berry and does Cicada powder is solid material, and the two is 50 according to quality:After 1 ratio mixes, insert in cordyceps sinensis cultivation tray, add nutrient solution, per 1kg Nutrient solution addition is 1.1L in solid material, 121 DEG C of sterilizing 20min~30min, inoculated and cultured after cooling；Inoculum concentration is：Every thousand The inoculum concentration of Periostracum cicadae liquid seeds is 50ml in gram solid material, after the inoculation of solid medium top layer, 20 DEG C of constant temperature illumination trainings Case 3~5d of light culture is supported, treats that mycelia covers with top layer, you can see optical culture, light intensity 1000Lux, daily illumination 12h；Illumination is trained 20d is supported, solid culture matrix grows a large amount of cicada fungus coremiums and starts to produce spore, collects cicada fungus coremium, conidia powder, culture Matrix；Dry cicada powder described in step (2) obtains for cicada larva crushed after being dried；Nutrient solution described in step (2) is 5% cider and 5% bananas juice volume ratio 1:4 mixing obtain；Hot water extraction, homogenate, are comprised the following steps that after coarse crushing described in step (3)：By fresh 2~8 parts of cicada fungus, fresh burdock root 10 ~15 parts, 2~8 parts of stir-baked RHIZOMA POLYGONATI with yellow rice wine, 3~5 parts of jujube, 1~5 part of Hericium erinaceus, 1~5 part of the fruit of Chinese wolfberry, 1~4 part of Chinese yam, light wheat 1~ 4 parts of ratio mixes raw material, and using pulverizer comminuting matter, the granularity of crushing rear material reaches 80~100 mesh；Fresh cicada fungus described in step (3) is conidia powder, coremium, culture matrix mass ratio are 1:5:15；Wet method high shear ultramicro grinding described in step (4), is concretely comprised the following steps：Raw material after crushing is placed in reactor, with material Liquor ratio is 1:20~30 ratio adds pure water, is cleared up in intelligent microwave in instrument, at control pressure 0.3MPa, 80W~90W microwave 80s~120s is managed, the feed liquid after extracted uses high speed homogenizer homogenized 10min under the conditions of 5000r/min；Cellulase, amylase described in step (5), pectin enzyme hydrolysis, are concretely comprised the following steps：Feed liquid after homogenate is sheared through twin-stage The further micronized pulverization of formula colloid mill, make Solids Particle Diameter in feed liquid molten using citric acid-buffered sodium citrate less than 1 μm Material liquid pH value after liquid regulation homogenate is in the range of 5~7, is added enzyme and is digested, enzyme used in enzymolysis is cellulase 38 ~100U/g, 500~800U/g of amylase, 50~80U/g of pectase, shake under 40~50 DEG C of temperature conditionss to material constant temperature Swing 1~2h of enzymolysis, after enzymolysis, the enzymolysis liquid of acquisition is warming up to 80 DEG C and is incubated 10min with the activity of destructive enzyme；It is filtering, concentration described in step (6), filling, concretely comprise the following steps：Feed liquid after enzyme deactivation uses 0.45um ceramic membrane filter After clarifying treatment, after reverse osmosis membrane concentrates, sterile filling, the cicada fungus burdock oral liquid of rufous is produced；Specific preparation process is as follows：(1) Periostracum cicadae culture liquid seed culture medium, using 10% cider, 10% bananas juice, 10% murphy juice according to 1: 1:Inoculated and cultured after three kinds of liquid are mixed compounding, sterilized by 5 volume ratio, fluid nutrient medium loading 500ml triangular flasks, every three The bottled liquid measure in angle is 200ml, and sterilize 15min~20min in 121 DEG C, access cicada fungus conidia powder, inoculum concentration 0.05g conidia powders/ 200ml fluid nutrient mediums, 19 DEG C of 3~5d of isothermal vibration incubator culture；(2) solid culture of Periostracum cicadae uses wheat berry and dry cicada powder, and the two is 50 according to quality:After 1 ratio mixes, put Enter in cordyceps sinensis cultivation tray, add nutrient solution, be 1.1L per 1kg solid material nutrient solutions addition, 121 DEG C of 20min that sterilize~ 30min, inoculated and cultured after cooling；Inoculum concentration is：Every kilogram of solid material inoculum concentration is 50ml, after the inoculation of solid medium top layer, 20 DEG C of constant temperature illumination box 3~5d of light culture, treat that mycelia covers with top layer, you can see optical culture, light intensity 1000Lux, daily Illumination 12h；Illumination cultivation 20d, solid culture matrix grow a large amount of cicada fungus coremiums and start to produce spore, collect cicada fungus falx Beam, conidia powder, culture matrix；Described nutrient solution is 5% cider and 5% bananas juice volume ratio 1:4 mixing obtain；(3) by fresh 2~8 parts of cicada fungus, 10~15 parts of fresh burdock root, 2~8 parts of stir-baked RHIZOMA POLYGONATI with yellow rice wine, 3~5 parts of jujube, 1~5 part of Hericium erinaceus, 1~5 part of the fruit of Chinese wolfberry, 1~4 part of Chinese yam, the ratio of 1~4 part of light wheat mix raw material, using pulverizer comminuting matter, crush The granularity of material reaches 80~100 mesh afterwards；Described fresh cicada fungus is conidia powder, coremium, culture matrix mass ratio are 1:5:15；(4) raw material after crushing is placed in reactor, using solid-liquid ratio as 1:20~30 ratio adds pure water, disappears in intelligent microwave Xie Yizhong, control pressure 0.3MPa, 80W~90W microwave treatment 80s~120s, the feed liquid after extracted use high speed homogenizer The homogenized 10min under the conditions of 5000r/min；(5) feed liquid after being homogenized makes Solids Particle Diameter in feed liquid be less than 1 through the further micronized pulverization of two-stage shear-type colloid mill μm, in the range of being in 5~7 using the material liquid pH value after citric acid-sodium citrate buffer regulation homogenate, add enzyme and carry out Enzymolysis, enzyme used in enzymolysis are 38~100U/g of cellulase, 500~800U/g of amylase, 50~80U/g of pectase, in 40~ To material 1~2h of vibrating at constant temperature for enzymolysis under 50 DEG C of temperature conditionss, after enzymolysis, the enzymolysis liquid of acquisition is warming up to 80 DEG C and is incubated 10min is with the activity of destructive enzyme；(6) after the feed liquid after enzyme deactivation is using 0.45um ceramic membrane filter clarifying treatment, after reverse osmosis membrane concentrates, sterile filling Dress, produce the cicada fungus burdock oral liquid of rufous.
- 2. a kind of cicada fungus burdock oral liquid is obtained as the preparation method described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510271479.7A CN104872758B (en) | 2015-05-25 | 2015-05-25 | The preparation method of cicada fungus burdock oral liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510271479.7A CN104872758B (en) | 2015-05-25 | 2015-05-25 | The preparation method of cicada fungus burdock oral liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104872758A CN104872758A (en) | 2015-09-02 |
| CN104872758B true CN104872758B (en) | 2018-03-16 |
Family
ID=53940651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510271479.7A Active CN104872758B (en) | 2015-05-25 | 2015-05-25 | The preparation method of cicada fungus burdock oral liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104872758B (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105227137B (en) * | 2015-10-14 | 2017-12-29 | 江南大学 | A kind of pesticide-germicide resonant cavity type radio-frequency signal generator |
| CN106797801B (en) * | 2015-11-26 | 2020-06-30 | 浙江泛亚生物医药股份有限公司 | Artificial culture method of cordyceps sobolifera |
| CN105433160A (en) * | 2015-12-03 | 2016-03-30 | 天益食品（徐州）有限公司 | Blood sugar-lowering burdock composite beverage and preparation method thereof |
| CN105614868A (en) * | 2016-02-07 | 2016-06-01 | 罗建华 | Isaria cicadae oral liquid and processing method thereof |
| CN108013269A (en) * | 2016-11-02 | 2018-05-11 | 浙江泛亚生物医药股份有限公司 | A kind of cicada fungus siberian solomonseal beverage and preparation method thereof |
| CN106923116A (en) * | 2017-02-19 | 2017-07-07 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of burdock fermented beverage |
| CN107080118A (en) * | 2017-04-17 | 2017-08-22 | 安徽虫草源生物科技有限公司 | A kind of Periostracum cicadae beverage and preparation method thereof |
| CN107183676A (en) * | 2017-04-17 | 2017-09-22 | 安徽虫草源生物科技有限公司 | A kind of Periostracum cicadae lozenge and preparation method thereof |
| CN107183677A (en) * | 2017-04-17 | 2017-09-22 | 安徽虫草源生物科技有限公司 | A kind of Periostracum cicadae oral liquid and preparation method thereof |
| CN107183678A (en) * | 2017-04-17 | 2017-09-22 | 安徽虫草源生物科技有限公司 | A kind of Periostracum cicadae instant powder and preparation method thereof |
| CN108419970A (en) * | 2018-01-29 | 2018-08-21 | 滁州学院 | A kind of chu chrysanthemum Periostracum cicadae fermented beverage and preparation method thereof |
| CN109953365A (en) * | 2019-03-22 | 2019-07-02 | 内蒙古昆明卷烟有限责任公司 | A kind of preparation method and application of cordyceps extracting liquid |
| CN109953366B (en) * | 2019-03-22 | 2022-11-11 | 内蒙古昆明卷烟有限责任公司 | Cordyceps sinensis extracting solution and preparation method, composition and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1393262A (en) * | 2001-07-02 | 2003-01-29 | 福州金大生物工程技术开发有限公司 | Process for preparing nutritive health-care oral liquid |
| CN1424053A (en) * | 2002-12-18 | 2003-06-18 | 上海医药工业研究院 | Capsule agent of chanhua mycelia and preparing method thereof |
| CN102552335A (en) * | 2011-02-22 | 2012-07-11 | 上海泛亚生命科技有限公司 | Traditional Chinese medicine health care product, its preparation method and its application |
| CN102805335A (en) * | 2012-07-20 | 2012-12-05 | 黄晓青 | Method for preparing edible fungus health-care product |
-
2015
- 2015-05-25 CN CN201510271479.7A patent/CN104872758B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1393262A (en) * | 2001-07-02 | 2003-01-29 | 福州金大生物工程技术开发有限公司 | Process for preparing nutritive health-care oral liquid |
| CN1424053A (en) * | 2002-12-18 | 2003-06-18 | 上海医药工业研究院 | Capsule agent of chanhua mycelia and preparing method thereof |
| CN102552335A (en) * | 2011-02-22 | 2012-07-11 | 上海泛亚生命科技有限公司 | Traditional Chinese medicine health care product, its preparation method and its application |
| CN102805335A (en) * | 2012-07-20 | 2012-12-05 | 黄晓青 | Method for preparing edible fungus health-care product |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104872758A (en) | 2015-09-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104872758B (en) | The preparation method of cicada fungus burdock oral liquid | |
| CN101617787B (en) | Process for continuously extracting a plurality of products from highland barley | |
| CN101451096B (en) | Method for preparing Chinese caterpillar fungus health wine | |
| CN101791119B (en) | Chaga nutrient composition | |
| CN109717340A (en) | A kind of fermentation preparation of the two-step Cordyceps militaris ferment of combination complex enzyme hydrolysis | |
| CN103315362B (en) | Poria cocos solid state fermentation functional beverage and its preparation method | |
| CN104970131A (en) | Health disease-treating, beauty-maintaining, young-keeping and long-life tea and preparation technology thereof | |
| CN102357212B (en) | Compound product obtained through supercritical CO2 extraction, and preparation method thereof | |
| CN103404557A (en) | Goat milk health-care bread | |
| CN105995489A (en) | Edible mushroom fermented corn flour and corn product | |
| CN105030846A (en) | Preparation method of cordyceps militaris superfine powder | |
| CN107400581A (en) | A kind of winter jujube beer and its preparation technology | |
| CN103330192B (en) | Preparation method of cordyceps militaris instant powder | |
| CN103315359B (en) | Grifola frondosus solid state fermentation functional beverage and its preparation method | |
| CN107080246A (en) | A kind of plant enzyme and preparation method thereof | |
| CN108782918A (en) | A kind of preparation method of pueraria lobata mulberries functionality composite fermentation pressed candy | |
| CN103070333B (en) | Cordyceps militaries (active substance) polysaccharide extraction and capsule preparation method | |
| CN107535934A (en) | A kind of Blueberry frozen and preparation method thereof | |
| CN103431100B (en) | Asparagus drink with health protection and health maintenance functions | |
| CN109463538A (en) | A kind of preparation method of milk cow small molecule feed peptide | |
| CN101972019A (en) | Facial beautification and health care function beverage made of radix polygonati officinalis and production method thereof | |
| CN101731576A (en) | Method for preparing nano-grade dietary fibers from burdock dregs | |
| CN103798520B (en) | A kind of feed additive of substitute antibiotics and preparation method thereof | |
| CN104547604A (en) | Preparation and processing technology of dendrobium candidum capsules | |
| CN106070879A (en) | A kind of Phellinus igniarius (L. ex Fr.) Quel. kudzu root tea and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 236000 Anhui Province, Bozhou City Qiaocheng District Wuma Industrial Park No. 88 Applicant after: Anhui Bo medicine Qiancao Chinese medicine Limited by Share Ltd Address before: 236000 Anhui Province, Bozhou City Qiaocheng District Wuma Industrial Park No. 88 Applicant before: Bozhou Qiancao Pharmaceutical Co., Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation method of cordyceps sobolifera and burdock oral liquid Effective date of registration: 20181129 Granted publication date: 20180316 Pledgee: Bozhou Yaodu Rural Commercial Bank Co., Ltd. Pledgor: Anhui Bo medicine Qiancao Chinese medicine Limited by Share Ltd Registration number: 2018340000685 |