CN1393262A - Process for preparing nutritive health-care oral liquid - Google Patents
Process for preparing nutritive health-care oral liquid Download PDFInfo
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- CN1393262A CN1393262A CN01119934A CN01119934A CN1393262A CN 1393262 A CN1393262 A CN 1393262A CN 01119934 A CN01119934 A CN 01119934A CN 01119934 A CN01119934 A CN 01119934A CN 1393262 A CN1393262 A CN 1393262A
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Abstract
A nutritive health-care oral liquid is prepared from cordyceps and other Chinese medicinal materials through extracting liquid extract from cordyceps through fermenting, crushing, immersion, enzymolyzing and filtering, and extracting the liquid extract of the said other Chinese medicinal materials through crushing, immersing and filtering, and mixing. Its advantages are high effect on improving immunity, preventing cancer and delaying sanility, and low cost.
Description
The present invention relates to a kind of preparation method of Nutrious health-care oral tonic
The patent No. is that the patent of invention of ZL96119701.3 discloses a kind of " cordyceps sinensis health-care nutrient liquor that tank fermentation method is produced ", and its major defect one is the hypotrophy face, and health-care effect is limited; The 2nd, production cost is higher.
The objective of the invention is to provide a kind of preparation method of Cordyceps Nutrious health-care oral tonic, the manufactured goods of this method are easily absorbed by human body, and nutrition and health-care effect are good.And helping the industrialization continuous production, production cost is lower.
The preparation method of Nutrious health-care oral tonic of the present invention is: by Cordyceps submerged fermentation extracting solution, the Mycophyta extracting solution that makes through pulverizing, lixiviate, enzymolysis and filtration and through pulverizing, lixiviate with to filter the extracts of Chinese herbal medicine that makes be that primary raw material is mixed with.
The oral liquid prescription of the embodiment of the invention can be: Cordyceps submerged fermentation extracting solution 60%; Ganoderma 5%; Tremella 3%; Auricularia 3%; Caulis Bambusae In Taeniam 3%; Tricholoma matsutake (lto et lmai) Singer 3%; Radix Ginseng 2%; The Radix Astragali 2%; Rhizoma Polygonati 3%; Fructus Lycii 3%; Radix Polygoni Multiflori 3; Herb Gynostemmae Pentaphylli 5%; Radix Codonopsis 2%; Radix Glycyrrhizae 1; Lac regis apis 2%.
The present invention is owing to be with medicinal fungi Cordyceps submerged fermentation extracting solution, the Mycophyta extracting solution, and extracts of Chinese herbal medicine is a primary raw material, so not only contain cordycepic acid than existing " cordyceps sinensis health-care nutrient liquor that tank fermentation method is produced ", cordycepin, twenty reveals alcohol, nutritional labeling and drug actions such as polysaccharide have strengthening the body resistance, and tonify deficiency is treated and decreased, nourishing the lung and kidney, spleen invigorating is calmed the nerves, blood fat reducing, blood pressure lowering, functions such as antitumor and defying age work, and will produce that cooperative effect is easier to be absorbed by human body.
Below in conjunction with embodiment the present invention is specifically described.
(1) oral liquid prescription of the embodiment of the invention is: Cordyceps submerged fermentation extracting solution 60%; Ganoderma 5%; Tremella 3%; Auricularia 3%; Caulis Bambusae In Taeniam 3%; Tricholoma matsutake (lto et lmai) Singer 3%; Radix Ginseng 2%; The Radix Astragali 2%; Rhizoma Polygonati 3%; Fructus Lycii 3%; Radix Polygoni Multiflori 3%; Herb Gynostemmae Pentaphylli 5%; Radix Codonopsis 2%; Radix Glycyrrhizae 1%; Lac regis apis 2%.
(2) step of preparation process of the present invention
(1) adopts tank fermentation method to produce Cordyceps submerged fermentation extracting solution, and take by weighing by the proportional quantity of the oral liquid prescription of the embodiment of the invention
(2) Cordyceps submerged fermentation extracting solution is standby.
(2) preparation Mycophyta extracting solution
A, take by weighing Ganoderma, Tremella, Auricularia by formulation ratio, Caulis Bambusae In Taeniam, Tricholoma matsutake (lto et lmai) Singer sporophore is ground into the granule of 0.5-1mm with disintegrating machine, drops into rustless steel and extracts in the pot, and add 10-14 and doubly purify waste water and soaked 1-2 hour,
B, advance a usefulness beater and smash, to improve extracting effect.
C, pH transfer to 6.5.
D, enzymolysis: in order to improve dissolution rate, add compound protease and cellulase, the protease dosage is 4 * 10
5U/kg, cellulase addition are 1.5 * 10
5U/kg.Temperature remains on 50-60 ℃, enzymolysis lixiviate 2-3h.
95-100 ℃ of sterilization of the fungus material heating 10min of e, enzymolysis makes enzyme deactivation.
Coarse filtration is carried out in f, filtration earlier, removes residue, and reuse fine filtering cloth fine straining is removed the fine particle of suspension.Collect filtrate for later use.
(3) preparation extracts of Chinese herbal medicine:
Other herbal raw material pulverize separately becomes diameter 2.0-4.0mm granule in will filling a prescription, and presses formulation ratio and drops in the extraction pot, adds 8-10 times of water, soaked 2-3 hour, and decocted filtration 2 hours in 100 ℃ then, filtering residue adds 8 times of water again and decocted filtration, merging filtrate 1.5 hours in 100 ℃.
(4) concentrate allotment:
With Cordyceps submerged fermentation extracting solution, Mycophyta and extracts of Chinese herbal medicine mix homogeneously inject the cryogenic vacuum concentration tank and concentrate, and are that to be evaporated to solid content under the 0.09Mpa about about 10% in vacuum, add 2% Lac regis apis.Fully stir, transfer PH5.0-6.0,, be finished product after the assay was approved with sterilizing 30 minutes at 121 ℃ behind 100ml, the 250ml Brown Glass Brown glass bottles and jars only filling and capping.
Above-mentioned Cordyceps submerged fermentation extracting solution can be produced by following processing step:
1.1 strain: the peacilomyce hepiahi bacterium (Paecilomyces hepiali chen et Dai) that provides by institute of microbiology of the Chinese Academy of Sciences.
1.2 culture medium:
Slant medium: Rhizoma Solani tuber osi---glucose---agar culture medium (PDA): Rhizoma Solani tuber osi liquor 1000ml, glucose 20g, agar 20g; The PH nature.
Liquid fermentation medium:
Semen sojae atricolor powder 8-10%; Semen Maydis powder 1%; Sucrose 2-3%; Yeast powder 0.2%; Magnesium sulfate 0.2-0.4%; Sodium chloride 0.2-0.4%; Dipotassium hydrogen phosphate 0.5%.Surplus: water; PH: nature.
1.3 fermentation technology flow process:
1.4 condition of culture:
1. the inclined-plane that will newly inoculate is put in 25-27 ℃ and cultivates 5-7d down, and mycelia is covered with the inclined-plane.
2. fresh inclined-plane is inoculated in the 500ml triangular flask and contains the 150ml seed culture medium, in placing under the shaking table 180r/min rotating speed 25-27 ℃ of shaken cultivation 2-3d, the mycelia of as seen covering with white on the bottle wall.
3. seed tank seed culture, inoculum concentration 10%, temperature 25-27 ℃, mixing speed is 150-180r/min, ventilation is per minute 1: 0.5V/V, min0.3-0.5 * 10
5Pa cultivates 2-3d.
Seed need carry out purity test before entering fermentor cultivation, guarantee that seed does not have living contaminants.
4. fermentation culture: inoculum concentration 10%, temperature 25-27 ℃, mixing speed is 150-180r/min, ventilation is per minute 1: 0.5V/v.min, cultivates 2-3 days.Fermentation liquid retrogradation this moment, thalline begins self-dissolving, and PH reduces to 4.5-5.0, and the residual sugar amount is reduced to about 0.3%, is fermentation termination when adenosine content begins to descend.
5. homogenizing is handled: earlier through the colloid mill homogenizing, the gap between its rotation mill and the fixing mill generally is no more than 0.3mm with the Cordyceps fermentation culture, rotation mill rotating speed 800-1000r/min.Make material particles dispersion disintegrates such as mycelium, thereby reach homogenizing, and then, make feed liquid under the high pressure effect, reach homogenizing and handle by the close clearance that generally is no more than 0.1mm through high pressure homogenizer (40Mpa-60Mpa).
The present invention has following remarkable advantage:
1, this oral liquid is rich in 17 kinds with upper amino acid, and especially arginine content is up to 92mg/100ml, multivitamin, mineral matter, trace Element and various bioactivators, such as nucleic acid, isoflavones, panaxoside, sweet mellow wine etc., its comprehensive nutrition has general dietotherapy and protects Strong effect improves and strengthens immunity of organisms and resistance against diseases.
2, the present invention is take medicinal fungi Deep Fermentation of Cordyceps sinensis Sacc extract as primary raw material, its same cordycepic acid that all contains with natural cs, Cordycepin, twenty reveals alcohol, nutritional labeling and the drug actions such as polysaccharide. Have equally and strengthen the body resistance to consolidate the constitution, qi-restoratives is treated and is decreased, nourishing the lung and kidney, and invigorating the spleen is calmed the nerves, Lipopenicillinase, step-down, antitumor and anti-aging effects.
3, Deep Fermentation of Cordyceps sinensis Sacc technology of the present invention foreshortens to 2-3 days with fermentation period, and technology has reached advanced international level, in addition, produces Technology can realize that industrialization produces continuously, so production cost is lower.
4. Nutrious health-care oral tonic collection Cordyceps of the present invention, the comprehensive effect of Chinese herbal medicine and fungi plant, and the generation cooperative effect is unprecedented.
Claims (3)
1, a kind of preparation method of Nutrious health-care oral tonic, it is characterized in that: by Cordyceps submerged fermentation extracting solution, the Mycophyta extracting solution that makes through pulverizing, lixiviate, enzymolysis and filtration and through pulverizing, lixiviate with to filter the extracts of Chinese herbal medicine that makes be that primary raw material is mixed with.
2, the preparation method of Nutrious health-care oral tonic according to claim 1 is characterized in that:
(1) oral liquid prescription: Cordyceps submerged fermentation extracting solution 60%; Ganoderma 5%; Tremella 3%; Auricularia 3%; Caulis Bambusae In Taeniam 3%; Tricholoma matsutake (lto et lmai) Singer 3% Radix Ginseng 2%; The Radix Astragali 2%; Rhizoma Polygonati 3%; Fructus Lycii 3%; Radix Polygoni Multiflori 3%; Herb Gynostemmae Pentaphylli 5%; Radix Codonopsis 2%; Radix Glycyrrhizae 1%; Lac regis apis 2%.
(2) step of preparation process
(1) adopt tank fermentation method to produce Cordyceps submerged fermentation extracting solution, and it is standby to take by weighing Cordyceps submerged fermentation extracting solution by the proportional quantity of oral liquid prescription.
(2) preparation Mycophyta extracting solution
A, take by weighing Ganoderma, Tremella, Auricularia by formulation ratio, Caulis Bambusae In Taeniam, Tricholoma matsutake (lto et lmai) Singer sporophore is ground into the granule of 0.5-1mm with disintegrating machine, drops into rustless steel and extracts in the pot, and add 10-14 and doubly purify waste water and soaked 1-2 hour,
B, advance a usefulness beater and smash, to improve extracting effect.
C, pH transfer to 6.5.
D, enzymolysis: in order to improve dissolution rate, add compound protease and cellulase, the protease dosage is 4 * 10
5U/kg, cellulase addition are 1.5 * 10
5U/kg.Temperature remains on 50-60 ℃, enzymolysis lixiviate 2-3h.
95-100 ℃ of sterilization of the fungus material heating 10min of e, enzymolysis makes enzyme deactivation.
Coarse filtration is carried out in f, filtration earlier, removes residue, and reuse fine filtering cloth fine straining is removed the fine particle of suspension.Collect filtrate for later use.
(3) preparation extracts of Chinese herbal medicine:
Other herbal raw material pulverize separately becomes diameter 2.0-4.0mm granule in will filling a prescription, and presses formulation ratio and drops in the extraction pot, adds 8-10 times of water, soaked 2-3 hour, and decocted filtration 2 hours in 100 ℃ then, filtering residue adds 8 times of water again and decocted filtration, merging filtrate 1.5 hours in 100 ℃.
(4) concentrate allotment:
With Cordyceps submerged fermentation extracting solution, Mycophyta and extracts of Chinese herbal medicine mix homogeneously inject the cryogenic vacuum concentration tank and concentrate, and are that to be evaporated to solid content under the 0.09Mpa about about 10% in vacuum, add 2% Lac regis apis.Fully stir, transfer PH5.0-6.0,, be finished product after the assay was approved with sterilizing 30 minutes at 121 ℃ behind 100ml, the 250ml Brown Glass Brown glass bottles and jars only filling and capping.
3. the preparation method of Nutrious health-care oral tonic according to claim 1 and 2 is characterized in that;
Described Cordyceps submerged fermentation extracting solution is produced by following processing step:
1.1 strain: the peacilomyce hepiahi bacterium (Paecilomyces hepiali chen et Dai) that provides by institute of microbiology of the Chinese Academy of Sciences;
1.2 culture medium:
Slant medium: Rhizoma Solani tuber osi---glucose---agar culture medium (PDA): Rhizoma Solani tuber osi liquor 1000ml, glucose 20g, agar 20g; The PH nature;
Liquid fermentation medium:
Semen sojae atricolor powder 8-10%; Semen Maydis powder 1%; Sucrose 2-3%; Yeast powder 0.2%; Magnesium sulfate 0.2-0.4%; Sodium chloride 0.2-0.4%; Dipotassium hydrogen phosphate 0.5%.Surplus: water; PH: nature;
1.3 fermentation technology flow process:
1.4 condition of culture:
1. the inclined-plane that will newly inoculate is put in 25-27 ℃ and cultivates 5-7d down, and mycelia is covered with the inclined-plane;
2. fresh inclined-plane is inoculated in the 500ml triangular flask and contains the 150ml seed culture medium, in placing under the shaking table 180r/min rotating speed 25-27 ℃ of shaken cultivation 2-3d, the mycelia of as seen covering with white on the bottle wall
3. seed tank seed culture, inoculum concentration 10%, temperature 25-27 ℃, mixing speed is 150-180r/min, ventilation is per minute 1: 0.5V/V, min0.3-0.5 * 10
5Pa cultivates 2-3d;
Seed need carry out purity test before entering fermentor cultivation, guarantee that seed does not have living contaminants;
4. fermentation culture: inoculum concentration 10%, temperature 25-27 ℃, mixing speed is 150-180r/min, ventilation is per minute 1: 0.5V/v.min, cultivates 2-3 days.Fermentation liquid retrogradation this moment, thalline begins self-dissolving, and PH reduces to 4.5-5.0, and the residual sugar amount is reduced to about 0.3%, is fermentation termination when adenosine content begins to descend;
5. homogenizing is handled: earlier through the colloid mill homogenizing, the gap between its rotation mill and the fixing mill generally is no more than 0.3mm with the Cordyceps fermentation culture, rotation mill rotating speed 800-1000r/min.Make material particles dispersion disintegrates such as mycelium, thereby reach homogenizing, and then, make feed liquid under the high pressure effect, reach homogenizing and handle by the close clearance that generally is no more than 0.1mm through high pressure homogenizer (40Mpa-60Mpa).
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Cited By (16)
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|---|---|---|---|---|
| CN102771776A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Ganoderma sinense health-care product containing traditional Chinese medicine extractives and preparation method of ganoderma sinense health-care product containing traditional Chinese medicine extractives |
| CN102805281A (en) * | 2011-05-31 | 2012-12-05 | 黑龙江省麒麟工贸公司 | Preparation method for tricholoma matsutake polysaccharide oral liquid |
| CN102805335A (en) * | 2012-07-20 | 2012-12-05 | 黄晓青 | Method for preparing edible fungus health-care product |
| CN103271334A (en) * | 2013-06-06 | 2013-09-04 | 吉林省起泰科技有限公司 | Cordyceps militaris-rana japonica nutrient liquid and preparation method thereof |
| CN103349315A (en) * | 2013-06-14 | 2013-10-16 | 朱奕 | Health-care oral liquid and preparation method thereof |
| CN103494197A (en) * | 2013-10-09 | 2014-01-08 | 吉林省现代中药工程研究中心有限公司 | Health-care food with functions of enhancing immunity and assisting to reduce blood sugar and preparation method of food |
| CN103504444A (en) * | 2012-06-16 | 2014-01-15 | 唐桂德 | Method for preparing mythic fungus and dictyophora phalloidea solid beverage |
| CN104126797A (en) * | 2014-07-28 | 2014-11-05 | 陈君亮 | High efficient utilization method of cordyceps sinensis fermentation liquor |
| CN104643225A (en) * | 2015-03-10 | 2015-05-27 | 邵素英 | Liquorice vegetable drink for improving gastrointestinal function and enhancing immunity and preparation method of liquorice vegetable drink |
| CN104664513A (en) * | 2015-03-11 | 2015-06-03 | 邵素英 | Licorice plant beverage and preparation method thereof |
| CN104872758A (en) * | 2015-05-25 | 2015-09-02 | 亳州千草药业有限公司 | Preparation method of cordyceps sobolifera and burdock oral liquid |
| CN105695247A (en) * | 2014-11-27 | 2016-06-22 | 衡阳市旺人食品实业有限公司 | Radix astragali chrysanthemum health care wine and preparation method thereof |
| CN106074713A (en) * | 2016-08-10 | 2016-11-09 | 翔天农业开发集团股份有限公司 | A kind of antihypertensive health care oral liquid and preparation method thereof |
| CN106173661A (en) * | 2016-07-13 | 2016-12-07 | 淮南恒天生物科技有限公司 | A kind of composite bacteria mixed fermentation drinks and preparation method thereof |
| CN106434380A (en) * | 2016-10-14 | 2017-02-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof |
| CN106520562A (en) * | 2016-10-14 | 2017-03-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for cultivating caterpillar fungus by using ginseng type extract and application |
-
2001
- 2001-07-02 CN CN01119934A patent/CN1393262A/en active Pending
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| CN103504444A (en) * | 2012-06-16 | 2014-01-15 | 唐桂德 | Method for preparing mythic fungus and dictyophora phalloidea solid beverage |
| CN102805335B (en) * | 2012-07-20 | 2015-07-29 | 北京厚文知识产权顾问有限公司 | A kind of preparation method of edible fungus health product |
| CN102805335A (en) * | 2012-07-20 | 2012-12-05 | 黄晓青 | Method for preparing edible fungus health-care product |
| CN102771776A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Ganoderma sinense health-care product containing traditional Chinese medicine extractives and preparation method of ganoderma sinense health-care product containing traditional Chinese medicine extractives |
| CN103271334A (en) * | 2013-06-06 | 2013-09-04 | 吉林省起泰科技有限公司 | Cordyceps militaris-rana japonica nutrient liquid and preparation method thereof |
| CN103349315A (en) * | 2013-06-14 | 2013-10-16 | 朱奕 | Health-care oral liquid and preparation method thereof |
| CN103494197A (en) * | 2013-10-09 | 2014-01-08 | 吉林省现代中药工程研究中心有限公司 | Health-care food with functions of enhancing immunity and assisting to reduce blood sugar and preparation method of food |
| CN104126797A (en) * | 2014-07-28 | 2014-11-05 | 陈君亮 | High efficient utilization method of cordyceps sinensis fermentation liquor |
| CN105695247A (en) * | 2014-11-27 | 2016-06-22 | 衡阳市旺人食品实业有限公司 | Radix astragali chrysanthemum health care wine and preparation method thereof |
| CN104643225A (en) * | 2015-03-10 | 2015-05-27 | 邵素英 | Liquorice vegetable drink for improving gastrointestinal function and enhancing immunity and preparation method of liquorice vegetable drink |
| CN104664513A (en) * | 2015-03-11 | 2015-06-03 | 邵素英 | Licorice plant beverage and preparation method thereof |
| CN104872758A (en) * | 2015-05-25 | 2015-09-02 | 亳州千草药业有限公司 | Preparation method of cordyceps sobolifera and burdock oral liquid |
| CN104872758B (en) * | 2015-05-25 | 2018-03-16 | 安徽亳药千草国药股份有限公司 | The preparation method of cicada fungus burdock oral liquid |
| CN106173661A (en) * | 2016-07-13 | 2016-12-07 | 淮南恒天生物科技有限公司 | A kind of composite bacteria mixed fermentation drinks and preparation method thereof |
| CN106074713A (en) * | 2016-08-10 | 2016-11-09 | 翔天农业开发集团股份有限公司 | A kind of antihypertensive health care oral liquid and preparation method thereof |
| CN106434380A (en) * | 2016-10-14 | 2017-02-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof |
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