CN106173661A - A kind of composite bacteria mixed fermentation drinks and preparation method thereof - Google Patents
A kind of composite bacteria mixed fermentation drinks and preparation method thereof Download PDFInfo
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- CN106173661A CN106173661A CN201610549065.0A CN201610549065A CN106173661A CN 106173661 A CN106173661 A CN 106173661A CN 201610549065 A CN201610549065 A CN 201610549065A CN 106173661 A CN106173661 A CN 106173661A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to a kind of composite bacteria mixed fermentation drinks and preparation method thereof, every 1000mL pure water comprises: Poria 9 ~ 15g, Caulis Bambusae In Taeniam 10 ~ 30g, Tremella aurantialba Bandoni et Zang 6 ~ 12g, Grifola frondosa 10 ~ 20g, Hericium erinaceus (Bull. Ex Fr.) Pers. 10 ~ 30g, Armillaria mellea 30 ~ 60g, Schizophyllum commune Franch． 9 ~ 16g, sweeting agent 1 ~ 5g, acidity regulator 0.1g ~ 0.5g, thickening agent and stabilizer 0.1g ~ 0.5g.Solid content is not less than 10%, total acid ＜ 1.0%, and color and luster is yellowish-brown, uniformity, is long placed in and is not susceptible to precipitation, has the distinctive fragrance of fermentation and flavour, and sweetness and bitterness is suitable, and fine and smooth coordination, mouthfeel is plentiful, and unique style is pure.Its preparation method includes: 1. preparation composite fungi superfine powder, 2. activated spawn, the 3. single bacterium fermentation seed liquid of preparation, 4. composite bacteria packet mixed fermentation, 5. homogenizing, allotment, canned.
Description
Technical field
The present invention relates to drinks field, specifically a kind of composite bacteria mixed fermentation drinks and preparation side thereof
Method.
Background technology
Integration of edible and medicinal herbs fungal food has nutritive value and health care.Integration of edible and medicinal herbs fungus itself is containing abundant albumen
Matter and cellulose, relatively low carbohydrate and fat, 8 kinds of aminoacid of needed by human, more than 30 kind vitamin, mineral etc. are sought
Forming point, these compositions are to the metabolism of human body and maintain normal vital movement all to play very important effect.Medicine is eaten
The trophic function composition that homology fungus has determines its health care.Have now been found that the fungus polysaccharide in integration of edible and medicinal herbs fungus,
The nutrition such as glycoprotein, polypeptide, terpenoid, steroid, alkaloid, volatile oil, nucleic acid, 18 diluted acid derivants and various trace elements
Functional component has the plurality of health care functions such as resisting fatigue, defying age and regulation immunologic function.
According to the conservative estimation of China beverage industry association, the coming five years, average annual by holding 12% ~ 15% of China's beverage total output
Speedup develops.At present domestic and international market beverage salable is respectively provided with nutrition and holds concurrently health properties, and sales volume increases substantially.But this
In class beverage, less to the useful fermented beverage of health, edible fungi beverage proportion, and edible fungi beverage has again quite
A part directly uses sporophore to be raw material production, causes the nutrition of edible fungi and function to play to greatest extent.
The cultivation of integration of edible and medicinal herbs fungus uses solid culture mode, and three grades of level Four ground of the most traditional solid spawn are from generation to generation
Switching, one turn is exactly half a year, and in production, front fearness is polluted, and has a lingering fear aging, and very complicated is not suitable for producing on a large scale；The opposing party
The face low sprouting of solid spawn purity is slow, pollutes high, and mycelia is uneven, poor product quality, and management is costly.From 80
In age to 20th century, the expert of many scientific research institutions of China teaches the older generation to integration of edible and medicinal herbs fungus submerged fermentation, liquid spawn
And middle-size and small-size Zymolysis Equipment has been carried out repeatedly, deeply and diligent research, promoting for the socialization of China liquid spawn today should
With, establish solid foundation.
Mixed fermentation refers to that the one using the synergism of two or more microorganism jointly to complete certain sweat is sent out
Ferment technology, can improve the shortcoming that single strain fermentation is not enough, and achieve in terms of food fermentation with production some achievements and
Application, the wine sour and sweet palatability produced such as multi-strain fermentation, alcoholic strength is low, nutritious, and amino acid content is high；Use multiple aspergillosis
Bacterium makes soy sauce more effective than single use aspergillus oryzae, and amino-acid production rate and content of glutamic acid also significantly improve.Mixed fermentation
In the beverage be particularly true in yogurt class, exploitation without alcohol beverages, lack employing integration of edible and medicinal herbs fungus and carry out mixed fungus fermentation
Produce the product of beverage.
In recent years, we are by external environmental and individual's unbalanced dual pincer attack of nutrition, raw facing to various societies
The double barrel attack of pressure alive, the immunologic function of self is but quietly declining, and causes the health of a lot of people to all suffer from prestige greatly
The side of body.Therefore modern the most urgently craves for some functional foods with enhancing human body immunity power, resisting fatigue and defying age.Pass through
The most deeply excavate, it has been found that integration of edible and medicinal herbs fungus has at the aspect of enhancing human body immunity power, resisting fatigue and defying age
Significantly effect, just disclosure satisfy that the eager demand that people are current.
Summary of the invention
For effectively solving the problems referred to above, the invention discloses a kind of with short production cycle, cell age neat and consistent, inoculation convenient,
It is suitable for factorial praluction, and comprehensive nutrition, integrates nutritional labeling and the healthy ingredient of multiple needed by human body, both can expire
Foot consumer nutritional need, it is also possible to play a kind of composite bacteria mixed fermentation drinks and the preparation side thereof of health-care effect
Method, its technical scheme is as follows.
This beverage comprises in every 1000mL pure water: Poria 9 ~ 15g, Caulis Bambusae In Taeniam 10 ~ 30g, Tremella aurantialba Bandoni et Zang 6 ~ 12g, Grifola frondosa 10 ~
20g, Hericium erinaceus (Bull. Ex Fr.) Pers. 10 ~ 30g, Armillaria mellea 30 ~ 60g, Schizophyllum commune Franch． 9 ~ 16g, sweeting agent 1 ~ 5g, acidity regulator 0.1g ~ 0.5g, thickening
Agent and stabilizer 0.1g ~ 0.5g.Solid content is not less than 10%, total acid ＜ 1.0%, and color and luster is yellowish-brown, and uniformity is long placed in
Being not susceptible to precipitation, have the distinctive fragrance of fermentation and flavour, sweetness and bitterness is suitable, and fine and smooth coordination, mouthfeel is plentiful, and unique style is pure
Just.
Its preparation process is as follows: 1. preparation composite fungi superfine powder: choose Poria 9 ~ 15g, Caulis Bambusae In Taeniam 10 ~ 30g, Tremella aurantialba Bandoni et Zang 6 ~
12g, Grifola frondosa 10 ~ 20g, Hericium erinaceus (Bull. Ex Fr.) Pers. 10 ~ 30g, Armillaria mellea 30 ~ 60g, Schizophyllum commune Franch． 9 ~ 16g, clean and carry out superfine powder after drying
Broken, be divided into 2 parts standby.
2. activated spawn: choose peacilomyce hepiahi (Paecilomyces hepiali Chen et Dai, sp.
Nov, CICC 14065), Hirsutella hepiali Chen et Shen (Hirsutella hepiali Chen et Shen, CICC 14016), Ganoderma lucidum (Leyss. Ex Fr.) Karst.
(Ganoderma lucidum, CICC 50006), the bacterial strain of Ganoderma (Ganoderma sinense, CICC 14049) turn respectively
Receive in PDA slant medium 27 ~ 29 DEG C to cultivate 5 days.PDA slant medium is: by peeling potatoes stripping and slicing 300 g, add
1000 mL distilled water, boil 10 ~ 20 min.By filtered through gauze, add glucose 10.0 ~ 20.0 g, agar 20.0 g, albumen
Peptone 5.0 g, potassium dihydrogen phosphate 1.0 g, magnesium sulfate (anhydrous) 0.5 g, heating dissolves, and adds distilled water to 1000 mL.Subpackage
After, 121 DEG C of sterilizing 20 min.
3. the single bacterium fermentation seed liquid of preparation: under sterile working's program, peacilomyce hepiahi, Hirsutella hepiali Chen et Shen, red
Sesame, the slant strains of Ganoderma cut into 1 cm3 fritter, choose the truffle after activation 2 ~ 4 pieces and are inoculated in 200mL liquid fermentation respectively
In culture medium, rotating speed is 25 DEG C of fermentation culture of constant-temperature table 7 days of 100 r/min.Single bacteria fermentation culture medium is: remove skin Ma Ling
Potato 200g, is cut into small pieces, and the 1.0L that adds water boils 30min, filters off potato ball, filtrate is complemented to 1.0L, adds glucose
20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, VB1 trace, agar 15.0g, pH6.0.After subpackage, 121 DEG C of sterilizings 20
min。
4. composite bacteria packet mixed fermentation: single bacterium fermentation seed liquid of peacilomyce hepiahi and Hirsutella hepiali Chen et Shen is pressed
The mixing of volume ratio 1:1 moves into fermentation tank A, is mixed by single bacterium fermentation seed liquid 1:1 by volume of Ganoderma lucidum (Leyss. Ex Fr.) Karst. and Ganoderma and moves into fermentation
Tank B, inoculum concentration is 10%, is continually fed into filtrated air, pressurize 0.03 ~ 0.1MPa, 23 ~ 25 DEG C of fermentation culture 10 ~ 15 days, will send out
It is standby that the fermentation liquid of ferment tank A and fermentation tank B is mixed to get beverage mother solution.Mixed fermentive culture medium is: composite fungi superfine powder 40 ~
90g, Semen Maydis powder 10 ~ 30g, analysis for soybean powder 2.5 ~ 10g, Semen arachidis hypogaeae dregs 2.5g, glucose 10 ~ 30g, sucrose 12 ~ 30g, peptone 2 ~
10g, yeast extract 0.5 ~ 10g, K2HPO4 1 ~ 3g, MgSO4 7H2O 0.5 ~ 1.5g, CaCl2 0.2 ~ 0.5g, VB1 0.25 ~
0.5g, the benefit that adds water to 1000mL, adjust pH 5.5 ~ 7.0 with saturated NaOH, adds polyoxypropylene ethylene oxide glycerin ether 0.03%, logical
Entering steam and be heated to 121 DEG C, 0.15 MPa keeps 30 ~ 45 min.
5. homogenizing, allotment, canned: after beverage mother solution and pure water 1: 2 ~ 5 mixings by volume, through high pressure homogenizer 20
~ 30 MPa homogenizing process, and cross its filtrate of leaching, add sweeting agent 1 ~ 5g, acidity regulator 0.1g ~ 0.5g, thickening agent and stablize
Agent 0.1g ~ 0.5g allotment, uses 30 ~ 50 DEG C of collaborative 400 ~ 600 MPa to keep 10 ~ 20min sterilization, after cold-aseptic filling subpackage
It is beverage product.
Selected by the present invention, raw material effect is as follows.
Peacilomyce hepiahi, the strain of Hirsutella hepiali Chen et Shen (are defended through Ministry of Health of the People's Republic of China and are sent out prison [2001]
" can be used for the fungus strain list of health food " has been listed in No. 84 literary composition approvals in, is natural " Cordyceps " artificial substituting product.Merit
Invigorating the lung and the kidney, hemostasis and phlegm can be cured mainly.For chronic cough dyspnea due to deficiency, chronic cough is spat blood, impotence and seminal emission, soreness of waist and knee joint.There is calmness, anti-frightened
Faint, blood pressure lowering, improves myocardial ischemia, defying age, improves immunity, two-ways regulation human endocrine, immunologic rejection, anti-lung cancer, lymph
Cancer, hepatocarcinoma function.
Ganoderma lucidum (Leyss. Ex Fr.) Karst., the strain of Ganoderma (are defended prison [2001] 84 literary composition approval to list in through Ministry of Health of the People's Republic of China
" can be used for the fungus strain list of health food ", be natural " Ganoderma " artificial substituting product.Nature and flavor are sweet, flat.Function cures mainly QI invigorating
Calm the nerves, relieving cough and asthma.For dizziness sleeplessness, shortness of breath and palpitation, asthenia cough with asthma.There is brain-strengthening, antiinflammatory, diuresis, stomach reinforcing, inhibiting cancer,
Blood pressure lowering, antithrombotic, strengthen immunologic function.
Poria: this product is the dry sclerotia of On Polyporaceae Poria.Nature and flavor are sweet, light, flat.Function cures mainly promoting diuresis to eliminate damp pathogen,
Spleen invigorating mind calming.For edema oliguria, phlegm retention vertigo and palpitation, insufficiency of the spleen lack of appetite, loose stool has loose bowels, irritability, palpitation with fear insomnia.There is cough-relieving,
Diuresis, calms the nerves, and brings down a fever, inhibiting cancer function.
Hericium erinaceus (Bull. Ex Fr.) Pers.: this product is hedgehog fungus section fungus Hericium erinaceus (Bull. Ex Fr.) Pers. and the sporophore of Hydnum.Nature and flavor are sweet；Flat.Function master
Control strengthening the spleen and nourishing the stomach, calm the nerves, anticancer.There is treatment gastric ulcer, neurasthenia, aid digestion, inhibiting cancer, defying age, blood, drops in blood sugar lowering
Fat, antithrombotic, improves immunity function.
Armillaria mellea: this product is the sporophore of Bai Mo section fungus armillariella tabescens.Nature and flavor are sweet, flat.Function cures mainly the suppressing the hyperactive liver that relieves dizziness, high fever, infantile convulsions, epilepsy, etc., and dispels
Wind and removing obstruction in channels, bone and muscle strengthening.There is calmness, enhancing immunity, treat neurasthenia, numb limbs and tense tendons, hepatopathy, insomnia, inhibiting cancer merit
Energy.
Grifola frondosa: this product is the sporophore of On Polyporaceae hen of the woods.Nature and flavor are sweet, flat.Function cures mainly QI invigorating and is good for
Spleen, qi-restoratives righting.There is treatment hepatopathy, diabetes, hypertension, inhibiting cancer, inhibiting HIV function.
Schizophyllum commune Franch．: this product is the sporophore of Bai Mo section fungus Schizophyllum commune Franch．.Nature and flavor are sweet, property is flat.Function cures mainly nourishing and fit keeping function, only
Band.There is treatment neurasthenia, antiinflammatory, inhibiting cancer function.
Tremella aurantialba Bandoni et Zang: this product is the sporophore of Tremellaceae fungus Tremella aurantialba Bandoni et Zang.Nature and flavor are sweet, flat.Function cures mainly nourishing YIN and moistening the lung, only promotes the production of body fluid
Yearningly.Have and reduce phlegm, cough-relieving, blood pressure lowering, inhibiting cancer, treat neurasthenia, the function such as asthma.
Caulis Bambusae In Taeniam: this product is Phallaceae fungus Cultivation of Dictyophora, short-skirted veiled lady, Dictyophora rubrovalvata and the sporophore of Dictyophora echino-volvata Zane.Property
Sweet in the mouth, cool.Function cures mainly boosting qi and nourishing yin, nourishing the lung to arrest cough, clearing away heat-damp and promoting diuresis.There is treatment dysentery, enhancing immunity, antibacterial, anti-ageing
Always, treat dysentery, reduce cholesterol, inhibiting cancer function.
Compared with prior art, the present invention has following technique effect.
1, composite bacteria mixed fermentation drinks has higher nutritive value and plurality of health care functions.Integration of edible and medicinal herbs is true
High protein that bacterium is well recognized as, low fat, homovitamin, high dietary-fiber, the health food of low sugar.True in integration of edible and medicinal herbs fungus
Granulose, glycoprotein, polypeptide, terpenoid, steroid, alkaloid, volatile oil, nucleic acid, 18 diluted acid derivants and lot of trace unit
The trophic function compositions such as element have the plurality of health care functions such as resisting fatigue, defying age and regulation immunologic function, play health care tool
There is the synergistic feature of globality, Mutiple Targets and multicomponent.
2, this beverage is by the selected feeding of multiple integration of edible and medicinal herbs fungus and scientific formula, convenient, give consumer rapidly
High nutrition, the drinks of absorption multi-functional, easy are provided.Composite bacteria mixed fermentation drinks solid content is not less than
10%, total acid ＜ 1.0%, color and luster is yellowish-brown, uniformity, is long placed in and is not susceptible to precipitation, has the distinctive fragrance of fermentation and taste
Taste, sweetness and bitterness is suitable, and fine and smooth coordination, mouthfeel is plentiful, and unique style is pure.
3, use submerged fermentation technology to produce integration of edible and medicinal herbs bacteria beverage, effect of integration of edible and medicinal herbs bacterium can be effectively maintained, with
Time degraded raw material in some compositions form many new nutrition and health care compositions, and produce many new flavor substances.Compound
Strain liquid mixed fermentation technology has that many bacterium symbiosis enzyme system is complementary, biochemical reaction is perfect, saving of labor is energy-conservation, shortens the production cycle,
Simplified process equipment, the advantage reducing production cost.
4, temperature-sensitive during the collaborative Technology of Ultra-high Pressure Sterilization of heat farthest ensure that the mouthfeel of product and color and luster, and material
The content of property trophic function composition and dissolution rate thereof.Caulis Bambusae In Taeniam fungus has contained natural antibacterial substance, and aseptic cold-penetration
The product of dress operation is not required to add preservative, thus ensure that the safety of product.
Detailed description of the invention
Now, the present invention will be further described in conjunction with the embodiments, but embodiment of the present invention is to the present invention
Explanation and the present invention can not be limited, any change in implication suitable with the present invention and scope and adjustment, be all considered as
In the scope of the present invention.
Embodiment 1: the present embodiment provides the preparation method of a kind of composite bacteria mixed fermentation drinks, including following step
Suddenly.
1. preparation composite fungi superfine powder: choose Poria 9g, Caulis Bambusae In Taeniam 10g, Tremella aurantialba Bandoni et Zang 6g, Grifola frondosa 10g, Hericium erinaceus (Bull. Ex Fr.) Pers. 10g, honey
Ring bacterium 30g, Schizophyllum commune Franch． 9g, clean dry after carry out micronizing, be divided into 2 parts standby.
2. activated spawn: choose peacilomyce hepiahi (Paecilomyces hepiali Chen et Dai, sp.
Nov, CICC 14065), Hirsutella hepiali Chen et Shen (Hirsutella hepiali Chen et Shen, CICC 14016), Ganoderma lucidum (Leyss. Ex Fr.) Karst.
(Ganoderma lucidum, CICC 50006), the bacterial strain of Ganoderma (Ganoderma sinense, CICC 14049) turn respectively
Receive in PDA slant medium 27 DEG C to cultivate 5 days.PDA slant medium is: by peeling potatoes stripping and slicing 300 g, add 1000
ML distilled water, boils 10 min.By filtered through gauze, add glucose 10.0 g, agar 20.0 g, peptone 5.0 g, di(2-ethylhexyl)phosphate
Hydrogen potassium 1.0 g, magnesium sulfate (anhydrous) 0.5 g, heating dissolves, and adds distilled water to 1000 mL.After subpackage, 121 DEG C of sterilizings 20
min。
3. the single bacterium fermentation seed liquid of preparation: under sterile working's program, peacilomyce hepiahi, Hirsutella hepiali Chen et Shen, red
Sesame, the slant strains of Ganoderma cut into 1 cm3 fritter, choose the truffle after activation 2 pieces and are inoculated in the training of 200mL liquid fermentation respectively
Supporting in base, rotating speed is 25 DEG C of fermentation culture of constant-temperature table 7 days of 100 r/min.Single bacteria fermentation culture medium is: take peeled potatoes
200g, is cut into small pieces, and the 1.0L that adds water boils 30min, filters off potato ball, filtrate is complemented to 1.0L, adds glucose
20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, VB1 trace, agar 15.0g, pH6.0.After subpackage, 121 DEG C of sterilizings 20
min。
4. composite bacteria packet mixed fermentation: single bacterium fermentation seed liquid of peacilomyce hepiahi and Hirsutella hepiali Chen et Shen is pressed
The mixing of volume ratio 1:1 moves into fermentation tank A, is mixed by single bacterium fermentation seed liquid 1:1 by volume of Ganoderma lucidum (Leyss. Ex Fr.) Karst. and Ganoderma and moves into fermentation
Tank B, inoculum concentration is 10%, is continually fed into filtrated air, pressurize 0.03MPa, and 23 DEG C of fermentation culture 10 days, by fermentation tank A and fermentation
It is standby that the fermentation liquid of tank B is mixed to get beverage mother solution.Mixed fermentive culture medium is: composite fungi superfine powder 40g, Semen Maydis powder 10g,
Analysis for soybean powder 2.5g, Semen arachidis hypogaeae dregs 2.5g, glucose 10g, sucrose 12g, peptone 2g, yeast extract 0.5g, K2HPO4 1g, MgSO4
7H2O 0.5g, CaCl2 0.2g, VB1 0.25g, the benefit that adds water to 1000mL, adjust pH 5.5 with saturated NaOH, add polyoxypropylene
Ethylene oxide glycerin ether 0.03%, is passed through steam and is heated to 121 DEG C, and 0.15 MPa keeps 30 min.
5. homogenizing, allotment, canned: after beverage mother solution and pure water 1: 2 mixing by volume, through high pressure homogenizer 20
MPa homogenizing processes, and crosses its filtrate of leaching, adds sweeting agent 1g, acidity regulator 0.1g, thickening agent and stabilizer 0.1g allotment,
Use 30 DEG C of collaborative 400 MPa to keep 10min sterilization, after cold-aseptic filling subpackage, be beverage product.
Embodiment 2: the present embodiment provides the preparation method of a kind of composite bacteria mixed fermentation drinks, including following step
Suddenly.
1. preparation composite fungi superfine powder: choose Poria 12g, Caulis Bambusae In Taeniam 15g, Tremella aurantialba Bandoni et Zang 9g, Grifola frondosa 15g, Hericium erinaceus (Bull. Ex Fr.) Pers. 15g,
Armillaria mellea 45g, Schizophyllum commune Franch． 13g, clean dry after carry out micronizing, be divided into 2 parts standby.
2. activated spawn: choose peacilomyce hepiahi (Paecilomyces hepiali Chen et Dai, sp.
Nov, CICC 14065), Hirsutella hepiali Chen et Shen (Hirsutella hepiali Chen et Shen, CICC 14016), Ganoderma lucidum (Leyss. Ex Fr.) Karst.
(Ganoderma lucidum, CICC 50006), the bacterial strain of Ganoderma (Ganoderma sinense, CICC 14049) turn respectively
Receive in PDA slant medium 28 DEG C to cultivate 5 days.PDA slant medium is: by peeling potatoes stripping and slicing 300 g, add 1000
ML distilled water, boils 15 min.By filtered through gauze, add glucose 15.0 g, agar 20.0 g, peptone 5.0 g, di(2-ethylhexyl)phosphate
Hydrogen potassium 1.0 g, magnesium sulfate (anhydrous) 0.5 g, heating dissolves, and adds distilled water to 1000 mL.After subpackage, 121 DEG C of sterilizings 20
min。
3. the single bacterium fermentation seed liquid of preparation: under sterile working's program, peacilomyce hepiahi, Hirsutella hepiali Chen et Shen, red
Sesame, the slant strains of Ganoderma cut into 1 cm3 fritter, choose the truffle after activation 3 pieces and are inoculated in the training of 200mL liquid fermentation respectively
Supporting in base, rotating speed is 25 DEG C of fermentation culture of constant-temperature table 7 days of 100 r/min.Single bacteria fermentation culture medium is: take peeled potatoes
200g, is cut into small pieces, and the 1.0L that adds water boils 30min, filters off potato ball, filtrate is complemented to 1.0L, adds glucose
20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, VB1 trace, agar 15.0g, pH6.0.After subpackage, 121 DEG C of sterilizings 20
min。
4. composite bacteria packet mixed fermentation: single bacterium fermentation seed liquid of peacilomyce hepiahi and Hirsutella hepiali Chen et Shen is pressed
The mixing of volume ratio 1:1 moves into fermentation tank A, is mixed by single bacterium fermentation seed liquid 1:1 by volume of Ganoderma lucidum (Leyss. Ex Fr.) Karst. and Ganoderma and moves into fermentation
Tank B, inoculum concentration is 10%, is continually fed into filtrated air, pressurize 0.06MPa, 23 ~ 25 DEG C of fermentation culture 13 days, by fermentation tank A and
It is standby that the fermentation liquid of fermentation tank B is mixed to get beverage mother solution.Mixed fermentive culture medium is: composite fungi superfine powder 60g, Semen Maydis powder
20g, analysis for soybean powder 7g, Semen arachidis hypogaeae dregs 2.5g, glucose 20g, sucrose 21g, peptone 6g, yeast extract 6g, K2HPO4 2g, MgSO4
7H2O 1g, CaCl2 0.3g, VB1 0.4g, the benefit that adds water to 1000mL, adjust pH 6.3 with saturated NaOH, add polyoxypropylene oxygen
Changing Ethylene Glycol ether 0.03%, be passed through steam and be heated to 121 DEG C, 0.15 MPa keeps 40 min.
5. homogenizing, allotment, canned: after beverage mother solution and pure water 1: 3 mixing by volume, through high pressure homogenizer 25
MPa homogenizing processes, and crosses its filtrate of leaching, adds sweeting agent 3g, acidity regulator 0.3g, thickening agent and stabilizer 0.3g allotment,
Use 40 DEG C of collaborative 500 MPa to keep 15min sterilization, after cold-aseptic filling subpackage, be beverage product.
Embodiment 3: the present embodiment provides the preparation method of a kind of composite bacteria mixed fermentation drinks, including following step
Suddenly.
1. preparation composite fungi superfine powder: choose Poria 15g, Caulis Bambusae In Taeniam 30g, Tremella aurantialba Bandoni et Zang 12g, Grifola frondosa 20g, Hericium erinaceus (Bull. Ex Fr.) Pers. 30g,
Armillaria mellea 60g, Schizophyllum commune Franch． 16g, clean dry after carry out micronizing, be divided into 2 parts standby.
2. activated spawn: choose peacilomyce hepiahi (Paecilomyces hepiali Chen et Dai, sp.
Nov, CICC 14065), Hirsutella hepiali Chen et Shen (Hirsutella hepiali Chen et Shen, CICC 14016), Ganoderma lucidum (Leyss. Ex Fr.) Karst.
(Ganoderma lucidum, CICC 50006), the bacterial strain of Ganoderma (Ganoderma sinense, CICC 14049) turn respectively
Receive in PDA slant medium 29 DEG C to cultivate 5 days.PDA slant medium is: by peeling potatoes stripping and slicing 300 g, add 1000
ML distilled water, boils 20 min.By filtered through gauze, add glucose 20.0 g, agar 20.0 g, peptone 5.0 g, di(2-ethylhexyl)phosphate
Hydrogen potassium 1.0 g, magnesium sulfate (anhydrous) 0.5 g, heating dissolves, and adds distilled water to 1000 mL.After subpackage, 121 DEG C of sterilizings 20
min。
3. the single bacterium fermentation seed liquid of preparation: under sterile working's program, peacilomyce hepiahi, Hirsutella hepiali Chen et Shen, red
Sesame, the slant strains of Ganoderma cut into 1 cm3 fritter, choose the truffle after activation 4 pieces and are inoculated in the training of 200mL liquid fermentation respectively
Supporting in base, rotating speed is 25 DEG C of fermentation culture of constant-temperature table 7 days of 100 r/min.Single bacteria fermentation culture medium is: take peeled potatoes
200g, is cut into small pieces, and the 1.0L that adds water boils 30min, filters off potato ball, filtrate is complemented to 1.0L, adds glucose
20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, VB1 trace, agar 15.0g, pH6.0.After subpackage, 121 DEG C of sterilizings 20
min。
4. composite bacteria packet mixed fermentation: single bacterium fermentation seed liquid of peacilomyce hepiahi and Hirsutella hepiali Chen et Shen is pressed
The mixing of volume ratio 1:1 moves into fermentation tank A, is mixed by single bacterium fermentation seed liquid 1:1 by volume of Ganoderma lucidum (Leyss. Ex Fr.) Karst. and Ganoderma and moves into fermentation
Tank B, inoculum concentration is 10%, is continually fed into filtrated air, pressurize 0.1MPa, 23 ~ 25 DEG C of fermentation culture 15 days, by fermentation tank A with send out
It is standby that the fermentation liquid of ferment tank B is mixed to get beverage mother solution.Mixed fermentive culture medium is: composite fungi superfine powder 90g, Semen Maydis powder
30g, analysis for soybean powder 10g, Semen arachidis hypogaeae dregs 2.5g, glucose 30g, sucrose 30g, peptone 10g, yeast extract 10g, K2HPO4 3g,
MgSO4 7H2O 1.5g, CaCl2 0.5g, VB1 0.5g, the benefit that adds water to 1000mL, adjust pH 7.0 with saturated NaOH, adds poly-
Oxypropylene ethylene oxide glycerin ether 0.03%, is passed through steam and is heated to 121 DEG C, and 0.15 MPa keeps 45 min.
5. homogenizing, allotment, canned: after beverage mother solution and pure water 1: 5 mixing by volume, through high pressure homogenizer 30
MPa homogenizing processes, and crosses its filtrate of leaching, adds sweeting agent 5g, acidity regulator 0.5g, thickening agent and stabilizer 0.5g allotment,
Use 50 DEG C of collaborative 600 MPa to keep 20min sterilization, after cold-aseptic filling subpackage, be beverage product.
Claims (3)
1. a composite bacteria mixed fermentation drinks, it is characterised in that comprise in 1000mL pure water: Poria 9 ~ 15g, bamboo
Sweet-smelling grass 10 ~ 30g, Tremella aurantialba Bandoni et Zang 6 ~ 12g, Grifola frondosa 10 ~ 20g, Hericium erinaceus (Bull. Ex Fr.) Pers. 10 ~ 30g, Armillaria mellea 30 ~ 60g, Schizophyllum commune Franch． 9 ~ 16g, sweeting agent 1
~ 5g, acidity regulator 0.1g ~ 0.5g, thickening agent and stabilizer 0.1g ~ 0.5g, solid content is not less than 10%, total acid ＜
1.0%, color and luster is yellowish-brown, uniformity, is long placed in and is not susceptible to precipitation, has the distinctive fragrance of fermentation and flavour, and sweetness and bitterness is fitted
Preferably, fine and smooth coordination, mouthfeel is plentiful, and unique style is pure.
A kind of composite bacteria mixed fermentation drinks the most according to claim 1, it is characterised in that preparation method includes
Following steps:
1. preparation composite fungi superfine powder: choose Poria 9 ~ 15g, Caulis Bambusae In Taeniam 10 ~ 30g, Tremella aurantialba Bandoni et Zang 6 ~ 12g, Grifola frondosa 10 ~ 20g, hedgehog hydnum
Bacterium 10 ~ 30g, Armillaria mellea 30 ~ 60g, Schizophyllum commune Franch． 9 ~ 16g, clean dry after carry out micronizing, be divided into 2 parts standby；
2. activated spawn: choose peacilomyce hepiahi (Paecilomyces hepiali Chen et Dai, sp. nov,
CICC 14065), Hirsutella hepiali Chen et Shen (Hirsutella hepiali Chen et Shen, CICC 14016), Ganoderma lucidum (Leyss. Ex Fr.) Karst.
(Ganoderma lucidum, CICC 50006), the bacterial strain of Ganoderma (Ganoderma sinense, CICC 14049) turn respectively
Receive in PDA slant medium 27 ~ 29 DEG C to cultivate 5 days；
PDA slant medium is: by peeling potatoes stripping and slicing 300 g, adds 1000 mL distilled water, boils 10 ~ 20 min, use yarn
Cloth filters, and adds glucose 10.0 ~ 20.0 g, agar 20.0 g, peptone 5.0 g, potassium dihydrogen phosphate 1.0 g, magnesium sulfate (nothing
Water) 0.5 g, heating dissolves, and adds distilled water to 1000 mL, after subpackage, 121 DEG C of sterilizing 20 min；
3. the single bacterium fermentation seed liquid of preparation: under sterile working's program, peacilomyce hepiahi, Hirsutella hepiali Chen et Shen, Ganoderma lucidum (Leyss. Ex Fr.) Karst., purple
The slant strains of sesame cuts into 1 cm3 fritter, chooses the truffle after activation 2 ~ 4 pieces and is inoculated in 200mL liquid fermentation and culture respectively
In base, rotating speed is 25 DEG C of fermentation culture of constant-temperature table 7 days of 100 r/min；
Single bacteria fermentation culture medium is: taking peeled potatoes 200g, be cut into small pieces, the 1.0L that adds water boils 30min, filters off Rhizoma Solani tuber osi
Block, complements to 1.0L by filtrate, adds glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, VB1 trace, fine jade
Fat 15.0g, pH6.0, after subpackage, 121 DEG C of sterilizing 20 min；
4. composite bacteria packet mixed fermentation: by single bacterium fermentation seed liquid of peacilomyce hepiahi and Hirsutella hepiali Chen et Shen by volume
Move into fermentation tank A than 1:1 mixing, single bacterium fermentation seed liquid 1:1 by volume of Ganoderma lucidum (Leyss. Ex Fr.) Karst. and Ganoderma mixed and moves into fermentation tank B,
Inoculum concentration is 10%, is continually fed into filtrated air, pressurize 0.03 ~ 0.1MPa, and 23 ~ 25 DEG C of fermentation culture 10 ~ 15 days, by fermentation tank A
To be mixed to get beverage mother solution standby with the fermentation liquid of fermentation tank B；
Mixed fermentive culture medium is: composite fungi superfine powder 40 ~ 90g, Semen Maydis powder 10 ~ 30g, analysis for soybean powder 2.5 ~ 10g, Semen arachidis hypogaeae dregs
2.5g, glucose 10 ~ 30g, sucrose 12 ~ 30g, peptone 2 ~ 10g, yeast extract 0.5 ~ 10g, K2HPO4 1 ~ 3g, MgSO4 7H2O
0.5 ~ 1.5g, CaCl2 0.2 ~ 0.5g, VB1 0.25 ~ 0.5g, the benefit that adds water to 1000mL, adjust pH 5.5 ~ 7.0 with saturated NaOH,
Adding polyoxypropylene ethylene oxide glycerin ether 0.03%, be passed through steam and be heated to 121 DEG C, 0.15 MPa keeps 30 ~ 45 min；
5. homogenizing, allotment, canned: after beverage mother solution and pure water 1: 2 ~ 5 mixings by volume, through high pressure homogenizer 20 ~ 30
MPa homogenizing processes, and crosses its filtrate of leaching, adds sweeting agent 1 ~ 5g, acidity regulator 0.1g ~ 0.5g, thickening agent and stabilizer
0.1g ~ 0.5g allocates, and uses 30 ~ 50 DEG C of collaborative 400 ~ 600 MPa to keep 10 ~ 20min sterilization, after cold-aseptic filling subpackage i.e.
For beverage product.
The preparation method of a kind of composite bacteria mixed fermentation drinks the most according to claim 2, it is characterised in that institute
Stating flavoring agent and include sweeting agent and acidity regulator, sweeting agent includes erythritol, maltose alcohol, xylitol, stevioside, sweet
One or more in grass glucin, aspartame, cyclamate, acesulfame potassium, alitame；Described acidity regulator include citric acid,
One or more in sodium citrate, malic acid；Described thickening agent and stabilizer include sucrose fatty acid ester, single double fats glycerol
One or more in ester, Konjac glucomannan, xanthan gum, carrageenan, gelatin, cycloheptaamylose, CMC-Na.
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| CN116210829A (en) * | 2022-12-27 | 2023-06-06 | 喜蕈生物科技有限公司 | Functional beverage and preparation method thereof |
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Application publication date: 20161207 |