CN110089651A - A kind of production method of capsicum leaf enzyme beverage - Google Patents

A kind of production method of capsicum leaf enzyme beverage Download PDF

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Publication number
CN110089651A
CN110089651A CN201910417991.6A CN201910417991A CN110089651A CN 110089651 A CN110089651 A CN 110089651A CN 201910417991 A CN201910417991 A CN 201910417991A CN 110089651 A CN110089651 A CN 110089651A
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capsicum leaf
ferment
capsicum
enzyme beverage
production method
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邱卫华
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Institute of Process Engineering of CAS
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Institute of Process Engineering of CAS
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • A23L2/74Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A23L21/25Honey; Honey substitutes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/31Leuconostoc
    • A23V2400/325Pseudomesenteroides

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Water Supply & Treatment (AREA)
  • Mycology (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

Capsicum leaf stock number is big, nutritive value is high, but does not obtain efficient high-value-use always.The present invention provides a kind of production method of capsicum leaf enzyme beverage, technical solution include the pretreatment of raw material, mixed raw material mashing ferment with homogeneous, the preparation for mixing ferment microbial inoculum inoculation liquid, capsicum leaf magma ferment, the allotment of capsicum leaf enzyme beverage.The present invention is by compounding aspergillus oryzae, lactic acid bacteria and saccharomycete as the mixed bacterial of capsicum enzyme beverage by core microbial inoculum of fermented tea, realize being precisely controlled for the plant enzyme fermentation based on capsicum leaf, production process is simple and easy to control, safety is not easy to pollute, and raw material utilization is more abundant, to keep active constituent and the exploitation high added value new product of capsicum leaf to provide new approach to greatest extent, industrialization prospect is wide.

Description

A kind of production method of capsicum leaf enzyme beverage
[technical field]
The invention belongs to fermented beverage production field, in particular to a kind of production method of capsicum leaf enzyme beverage.
[background technique]
China is the maximum country of pepper cultivation area in the world, while being also the maximum capsicum country of consumption in the whole world and outlet State.Capsicum leaf accounts for the overwhelming majority of pepper plant, therefore capsicum leaf resource very abundant.But it is considered as agricultural all the time Waste, the processing for it are mostly to burn, is discarded etc., have not only been unfavorable for the protection of environment, but also have ignored the application of capsicum leaf Value.And with favor of the people to green natural food in recent years, the rising star that capsicum leaf becomes in vegetables starts to flow in foreign countries Nutritional health food is developed using capsicum leaf in row, the ground such as Southeast Asia, Hong Kong of Japan and China, Macao.Studies have shown that capsicum The nutritional ingredient of leaf is better than pepper fruit.For total starch content 46.95% or more, total dietary fiber is close in capsicum leaf 40%, protein content may be up to 21.07%, in addition microelement also rich in, Flavonoid substances, fat, carbon aquation Close object, VC etc..External some researchers have carried out a series of research to the nutrition health-care functions of capsicum leaf.It was found that capsicum Leaf has anti-oxidant, antiallergy and the various actives such as antitumor.It can be seen that capsicum leaf is to collect edible, medicinal and health care is in one The wholefood of body.
In view of capsicum leaf nutrition abundant and health active, capsicum leaf is more and more processed into health care function Product.Tealeaves is made in capsicum leaf by patent ZL 201310431059.1 and 201710604676.5;Patent 201510813460.0 Then it is made by raw material of the extracting solution of fresh chilli leaf and mulberry leaf with anti-oxidant, protection eyesight, pre- radiation protection, reducing blood lipid, drop Plant leaf health care beverage effect of blood glucose;Patent 201210267362.8 is then to propose a kind of fruit and vegetable composite tea beverage and its system Preparation Method, the beverage have effects that reducing fever and causing diuresis, invigorating the spleen dehumidifying, promoting fluid production and nourishing stomach;Patent 201510771152.6 proposes capsicum leaf It takes liquid and honey powder to deploy be made with anti-cancer and cancer-preventing, reducing weight and beautifying features, refresh the mind, the function such as sharp intestines defaecation and nourishing liver moistening lung decompression The health drink of energy.But this product drunk that directly brews capsicum leaf utilizes in capsicum leaf with being obviously unable to fully Active constituent necessarily causes the waste of capsicum leaf resource.Also have biotechnology applications in the report of capsicum leaf production.Specially Benefit 201711405275.3 can be removed the jerky taste of capsicum leaf, be increased fragrance and the battalion of small molecule with lactic fermentation capsicum leaf It forms point, obtained chilli leaves tea mouthfeel is more mellow, and millet paste is aromatic, and color is yellowish green.Patent 201410749185.6 is then With golden flower bacterium fermented capsicum leaf or capsicum leaf extract, new type of health tealeaves or drink with peculiar fragrance and novel mouthfeel is made Product.Patent 201610373683.4 and 201711269524.0 increases fermented capsicum with lactic acid bacteria and saccharomycete pickled hot pepper leaf The natural flavor of leaf, and can inhibit varied bacteria growing, reduce salt and content of nitrite.But these methods are all also more low End and low value, limit the development of capsicum leaf industrial chain.
In recent years, the research and development in the fashionable whole world of ferment product, each fermentoid emerge one after another.Ferment is divided into nature strain hair Ferment and two kinds of artificial strain fermentation.The latter is mainly the hair that ferment is carried out using one or more known beneficial microbe strains Ferment, common fermenting microbe have saccharomycete, acetic acid bacteria and lactic acid bacteria etc..Food plant ferment is with one or more fresh vegetables The food materials such as dish, fruit, paddy beans, seaweeds, dual-purpose of drug and food book on Chinese herbal medicine class are raw material, are produced through multiple beneficial bacterium lengthy fermentation Functional microbial fermented product.Because of the growth and metabolism of microorganism itself, the metabolic conversion between substance is realized, because And also produce a large amount of enzymes with multiple biological activities and complicated metabolite or mesostate.Also therefore ferment is logical Often all have and promotes to digest and assimilate, adjust function of human body, improve the healthcare functions such as immunity of organisms.But there is not yet with capsicum leaf Develop the report of ferment product.
Fermented tea (Kombucha, tea mushroom tea fungus) is also commonly called as " Hypon " " stomach is precious " etc., is one Kind is by being rich in probiotics and work made of " symbiosis bacterium colony " the fermented tea syrup of compositions such as acetic acid bacteria, lactic acid bacteria and saccharomycete The product of property ingredient.Powerful symbiosis and complicated metabolic pathway are formd in fermented tea between microorganism.Wherein saccharomycete is Sucrose etc. is decomposed into glucose and fructose in the primary stage (0-3d) of fermentation, and further generated by " promoter " and bridge Ethyl alcohol and CO2;Acetic acid bacteria is that carbon source starts fast-growth and breeds, and generate acetic acid, gluconic acid etc. using glucose and fructose Metabolite;The substances such as vitamin and amino acid that lactic acid bacteria is generated using metabolism of yeasts are grown into breeding, and generate one Quantitative lactic acid.The presence of acetic acid, ethyl alcohol can protect fermentation system infecting from other bacterial strains.More and more research tables The fermentation of bright fermented tea can produce a large amount of active constituent, mainly include gluconic acid, acetic acid, glucose, fructose, protein, Amino acid, vitamin, microelement, tea polyphenols, caffeine, ethyl alcohol and carbon dioxide etc., also therefore fermented tea is to a variety of diseases All there is potential therapeutic effect, including anti-oxidant, carcinogenesis, anti-diabetic improves immunity, enhance liver detoxification ability, Facilitate the treatment etc. of gastric ulcer and high cholesterol.
It is an object of the invention to overcome existing capsicum leaf using theory and technical shortcoming, a kind of capsicum is provided The production method of leaf enzyme beverage, the effect of to give full play to active constituent in capsicum leaf and exploitation high added value new product provides New way.
[summary of the invention]
Capsicum leaf stock number is big, nutritive value is high, but does not obtain efficient high-value-use always.The present invention breaks through existing Utilization rate is low in capsicum leaf Land use systems, development technique low side, the problems such as value of the product is not high, provides a kind of capsicum leaf ferment drink The production method of material, the effect of to give full play to active constituent in capsicum leaf and exploitation high added value new product provides new way.
Technical scheme is as follows:
A kind of production method of capsicum leaf enzyme beverage of the present invention includes that the pretreatment of raw material, mixed raw material are beaten It starches and the steps such as homogeneous, the preparation for mixing ferment microbial inoculum inoculation liquid, the fermentation of capsicum leaf magma ferment, the allotment of capsicum leaf enzyme beverage Suddenly.It is specific to be realized by following steps:
1) pretreatment of raw material: choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, gently rinsed and removed with sterile water Remove the dust of capsicum leaf surface, room temperature hang airing surface moisture, by clean capsicum leaf in 90 DEG C of hot water blanching 10s, Other auxiliary material aseptic water washings are clean, and dry surface moisture;
2) mixed raw material mashing is with homogeneous: after taking pretreated capsicum leaf to mix with auxiliary material according to certain weight ratio, adding Water is beaten, obtains the magma that capsicum leaf ferment ferments after homogeneous;
3) it mixes the preparation of ferment microbial inoculum inoculation liquid: tealeaves being added according to w/v 1:50-200 (w/v, g/ml) 20min is extracted in 90 DEG C of -100 DEG C of hot water, filters to take millet paste;Millet paste pours into sterilized container, according still further to w/v 5%~20% (wt%) be added table sugar, stir sufficiently after inoculation mixing ferment microbial inoculum, sealed with gauze, 28-35 DEG C with After 180rpm shaken cultivation 2-3d, plastic covering cloth is sealed on gauze, in 28-35 DEG C of stationary culture 3-5d;
4) capsicum leaf magma ferment ferments: the capsicum leaf and auxiliary material magma 20~50% (w/v, similarly hereinafter) of homogeneous; Table sugar 5~20%, mixes ferment microbial inoculum inoculation liquid 10%~30%, and surplus sterile purified water is after mixing evenly, close with gauze Envelope, 28-35 DEG C with 180rpm shaken cultivation 2-5d after, on gauze plastic covering cloth seal, in 28-35 DEG C of stationary culture 5- 30d;
5) allotment of capsicum leaf enzyme beverage: the filtering of capsicum leaf ferment tunning takes the ferment fermentation liquid of clear, Then the fumet of 0.05-0.2% sweetener, 5% honey, 0.1~0.2% pectin, 0.01%-0.05% is added, stirring is equal It is stood after even more than for 24 hours, pours into clear glass bottle after then filtering with 0.2-0.45 μm of film, obtain capsicum leaf extract ferment drink Material.
Auxiliary material described in step 1) includes one or more of mushroom, lily, pineapple, pawpaw.Wherein mushroom is preferred Mushroom and Pleurotus eryngii.The preferred mushroom of auxiliary material and pineapple.
The weight of capsicum leaf accounts for 50-80% in mixed raw material described in step 2), the weight of auxiliary material accounts for 5~20%.It is excellent The capsicum leaf weight of choosing is 65%, and the weight of mushroom and pineapple is respectively 20% and 15% in auxiliary material.
The table sugar includes one or more of white granulated sugar, rock sugar, brown sugar, soft white sugar, honey, concentrated sugarcane juice Mixture.The table sugar addition concentration refers to the total concentration of several sugar, and when adding more than one sugar, every kind sugared Additive amount is identical.
The tealeaves includes one of green tea, black tea, dark green tea.
The mixing ferment microbial inoculum is independent or compounding aspergillus oryzae, lactic acid bacteria, yeast using fermented tea as core microbial inoculum One of bacterium is several.Black tea mother bacterial liquid above-mentioned is commercially available fermented tea, and lactic acid bacteria is lactobacillus plantarum, the bright string of false goldbeater's skin One or more of pearl bacterium, lactobacillus acidophilus, saccharomycete are in saccharomyces cerevisiae, Hansenula anomala and Bayer Zygosaccharomyces It is one or more.
The inoculation volume of ferment microbial inoculum inoculum above-mentioned is the 20%~50% of capsicum leaf virgin pulp liquid volume.
The sweetener is the composition of one or both of acesulfame potassium, stevioside.
The fumet is one or more of compositions of ethylmaltol, vanillic aldehyde, essence for food.
[advantages of the present invention]
The present invention has the advantage that
1) traditional ferment natural fermentation process is difficult to be precisely controlled, and the present invention is using fermented tea mixed bacterial as core Microbial inoculum, by formulations mixing ferment floras such as compounding aspergillus oryzae, lactic acid bacteria and saccharomycete, not only production process is simple and easy to control, Safety is not easy to pollute, and raw material utilization is more abundant, to keep active constituent and the exploitation of capsicum leaf high additional to greatest extent Value new product provides new approach, and industrialization prospect is wide.
2) plant enzyme beverage of the invention is to be aided with mushroom, pineapple, pawpaw etc. by major ingredient of capsicum leaf, and raw material itself is sought Feeding value is higher, while also containing including the enzymes abundant such as laccase, cellulase, protease, so that the nutriture value of ferment product It is worth higher.Beneficial bacterium symbiotic fermentation promotes a large amount of generations of beneficial metabolic product in ferment fermentation system, so that made Standby capsicum leaf extract enzyme beverage has special bioactivity.
[specific implementation method]
The present invention will be further described combined with specific embodiments below, but not exhaustive, those skilled in the art without The simple copy and improvement for crossing creative work belong to the range of the claims in the present invention protection.
Embodiment 1
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, is gently rinsed with sterile water remove remove capsicum leaf table The dust in face, room temperature hang airing surface moisture, by clean capsicum leaf in 90 DEG C of hot water blanching 10s, auxiliary material mushroom, spinach Trailing plants, lily are clean with aseptic water washing, and dry surface moisture;Take 50 parts of pretreated capsicum leaf and 20 parts of mushrooms and 15 parts After pineapple and 15 parts of lily mixing, adds water to be beaten, obtains the virgin pulp liquid of capsicum leaf ferment fermentation after homogeneous.
Green tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:50 (w/v, g/ml) and extracts 20min, is filtered Take millet paste;Millet paste pours into sterilized container, white granulated sugar is added according to w/v 15% (wt%), stirring is sufficiently followed by Kind mixing ferment microbial inoculum: black tea mother bacterial liquid, 21115 (Leuconostoc of leuconostoc pseudomesenteroides CICC Pseudomesenteroides), aspergillus oryzae CICC 40620 (Aspergillus oryzae, similarly hereinafter), saccharomyces cerevisiae CICC 1251 (Saccharomyces cerevisiae, similarly hereinafter), 32677 (Zygosaccharomyces of Bayer Zygosaccharomyces CICC Bailii, similarly hereinafter), sealed with gauze, 30 DEG C with 180rpm shaken cultivation 2-3d after, plastic covering cloth seals on gauze, In 30 DEG C of stationary culture 3-5d.
According to capsicum leaf ferment fermentation virgin pulp liquid 20% (wt%, similarly hereinafter), table sugar 5%, mixing ferment microbial inoculum inoculation liquid 10%~30%, surplus sterile purified water is sealed after mixing evenly with gauze, 30 DEG C with 180rpm shaken cultivation 2d after, Plastic covering cloth seals on gauze, after 30 DEG C of stationary culture 7d, filters to take the ferment fermentation liquid of clear, is then added 0.05% stevioside, 5% honey, 0.1% pectin, 0.01% vanillic aldehyde, stood more than for 24 hours after mixing evenly, then with Clear glass bottle is poured into after 0.2-0.45 μm of film filtering, obtains capsicum leaf extract enzyme beverage.
Embodiment 2
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, is gently rinsed with sterile water remove remove capsicum leaf table The dust in face, room temperature hang airing surface moisture, by clean capsicum leaf in 90 DEG C of hot water blanching 10s, auxiliary material pineapple, pawpaw It is clean with aseptic water washing, and dry surface moisture;65 parts of pretreated capsicum leaf are taken to mix with 15 portions of pineapples and 20 portions of pawpaws After conjunction, water added to be beaten, obtain the virgin pulp liquid of capsicum leaf ferment fermentation after homogeneous.
Black tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:100 (w/v, g/ml) and extracts 20min, is filtered Take millet paste;Millet paste pours into sterilized container, and the mixing of white granulated sugar and brown sugar is added according to w/v 10% (wt%) Object, inoculation mixing ferment microbial inoculum after stirring sufficiently: black tea mother bacterial liquid, lactobacillus plantarum plant subspecies CICC 20272 (Lactobacillus plantarum subsp.Plantarum, similarly hereinafter), aspergillus oryzae, Bayer Zygosaccharomyces, the inferior ferment of the abnormal Chinese Female CICC 1312 (Hansenula anomala var.anomala, similarly hereinafter), is sealed with gauze, is vibrated at 28 DEG C with 180rpm After cultivating 3d, plastic covering cloth is sealed on gauze, in 28 DEG C of stationary culture 5d.
It is connect according to capsicum leaf ferment fermentation virgin pulp liquid 40% (wt%, similarly hereinafter), concentrated sugarcane juice 10%, mixing ferment microbial inoculum Kind of liquid 20%, surplus sterile purified water are sealed after mixing evenly with gauze, 28 DEG C with 180rpm shaken cultivation 5d after, Plastic covering cloth seals on gauze, after 30 DEG C of stationary culture 14d, filters to take the ferment fermentation liquid of clear, is then added 0.1% stevioside, 5% honey, 0.1% pectin, 0.02% edible essence, stood more than for 24 hours after mixing evenly, then with Clear glass bottle is poured into after 0.2-0.45 μm of film filtering, obtains capsicum leaf extract enzyme beverage.
Embodiment 3
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, is gently rinsed with sterile water remove remove capsicum leaf table The dust in face, room temperature hang airing surface moisture, by clean capsicum leaf in 90 DEG C of hot water blanching 10s, auxiliary material pineapple nothing Bacterium water is rinsed well, and dries surface moisture;After taking 80 parts of pretreated capsicum leaf to mix with 20 parts of pineapple, water is added to be beaten The virgin pulp liquid of capsicum leaf ferment fermentation is obtained after slurry, homogeneous.
Green tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:100 (w/v, g/ml) and extracts 20min, is filtered Take millet paste;Millet paste pours into sterilized container, and the white granulated sugar and honey of equivalent are added according to w/v 20% (wt%) Mixture, inoculation mixing ferment microbial inoculum after stirring sufficiently: black tea mother bacterial liquid, lactobacillus plantarum plant subspecies, the false bright string of goldbeater's skin Pearl bacterium, aspergillus oryzae, Hansenula anomala, are sealed with gauze, 32 DEG C with 180rpm shaken cultivation 3d after, on gauze cover modeling Cloth sealing is expected, in 32 DEG C of stationary culture 5d.
According to capsicum leaf ferment fermentation virgin pulp liquid 50% (wt%, similarly hereinafter), the white granulated sugar of equivalent and honey mixt 10%, Ferment microbial inoculum inoculation liquid 30% is mixed, surplus sterile purified water is sealed after mixing evenly with gauze, at 32 DEG C with 180rpm After shaken cultivation 5d, plastic covering cloth is sealed on gauze, after 32 DEG C of stationary culture 30d, filters to take the ferment of clear Fermentation liquid, be then added 0.1% acesulfame potassium, 5% honey, 0.1% pectin, 0.02% ethylmaltol, it is quiet after mixing evenly It sets more than for 24 hours, pours into clear glass bottle after then filtering with 0.2-0.45 μm of film, obtain capsicum leaf extract enzyme beverage.
Embodiment 4
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, is gently rinsed with sterile water remove remove capsicum leaf table The dust in face, room temperature hang airing surface moisture, by clean capsicum leaf in 90 DEG C of hot water blanching 10s, auxiliary material mushroom, lily It is clean with aseptic water washing, and dry surface moisture;50 parts of pretreated capsicum leaf are taken to mix with 25 parts of mushroom and 25 parts of lily After conjunction, water added to be beaten, obtain the virgin pulp liquid of capsicum leaf ferment fermentation after homogeneous.
Green tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:100 (w/v, g/ml) and extracts 20min, is filtered Take millet paste;Millet paste pours into sterilized container, according to w/v 15% (wt%) be added the white granulated sugar of equivalent, rock sugar and The mixture of honey, inoculation mixing ferment microbial inoculum after stirring sufficiently: black tea mother bacterial liquid, leuconostoc pseudomesenteroides, aspergillus oryzae, Bayer Zygosaccharomyces are sealed with gauze, 30 DEG C with 180rpm shaken cultivation 2d after, on gauze plastic covering cloth seal, at 30 DEG C Stationary culture 5d.
According to the mixing of capsicum leaf ferment fermentation virgin pulp liquid 40% (wt%, similarly hereinafter), the brown sugar of equivalent and concentrated sugarcane juice Object 10%, mixing ferment microbial inoculum inoculation liquid 20%, surplus sterile purified water is sealed after mixing evenly with gauze, 35 DEG C with After 180rpm shaken cultivation 5d, plastic covering cloth is sealed on gauze, after 35 DEG C of stationary culture 15d, filters to take clear Ferment fermentation liquid, be then added 0.05% acesulfame potassium and 0.05% stevioside, 5% honey, 0.2% pectin, 0.01% food It with essence, is stood more than for 24 hours after mixing evenly, pours into clear glass bottle after then filtering with 0.2-0.45 μm of film, obtain capsicum Leaf extract enzyme beverage.
Embodiment 5
Choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, is gently rinsed with sterile water remove remove capsicum leaf table The dust in face, room temperature hang airing surface moisture, by clean capsicum leaf in 90 DEG C of hot water blanching 10s, auxiliary material Pleurotus eryngii, wood Melon, lily are clean with aseptic water washing, and dry surface moisture;Take 60 parts of pretreated capsicum leaf and 20 parts of Pleurotus eryngii, wood After 10 parts of melon, 10 parts of lily mixing, adds water to be beaten, obtains the virgin pulp liquid of capsicum leaf ferment fermentation after homogeneous.
Black tea is added in 90 DEG C of -100 DEG C of hot water according to w/v 1:50 (w/v, g/ml) and extracts 20min, is filtered Take millet paste;Millet paste pours into sterilized container, and equivalent white granulated sugar and brown sugar is added according to w/v 20% (wt%) Mixture, stir sufficiently after be inoculated with fermented tea mother liquor, sealed with gauze, 30 DEG C with 180rpm shaken cultivation 2d after, in gauze Upper plastic covering cloth sealing, in 30 DEG C of stationary culture 5d.
According to capsicum leaf ferment fermentation virgin pulp liquid 50% (wt%, similarly hereinafter), the 1:1 mixture 5%, red of white granulated sugar and brown sugar Tea mother bacterial liquid 20%, surplus sterile purified water are sealed after mixing evenly with gauze, at 30 DEG C with 180rpm shaken cultivation 5d Afterwards, plastic covering cloth seals on gauze, after 30 DEG C of stationary culture 15d, filters to take the ferment fermentation liquid of clear, then 0.2% stevioside, 5% honey, 0.1% pectin, 0.02% edible essence and 0.02% vanillic aldehyde is added, after mixing evenly It stands more than for 24 hours, pours into clear glass bottle after then filtering with 0.2-0.45 μm of film, obtain capsicum leaf extract enzyme beverage.

Claims (9)

1. a kind of production method of capsicum leaf enzyme beverage of the present invention, which is characterized in that pretreatment including raw material, mixed Close raw material pulping and homogeneous, the preparation for mixing ferment microbial inoculum inoculation liquid, capsicum leaf magma ferment ferment, capsicum leaf enzyme beverage Allotment and etc..
2. a kind of production method of capsicum leaf enzyme beverage according to claim 1, it is characterised in that be by following step Suddenly it realizes:
1) pretreatment of raw material: choose without blackspot, withered spot, notch, worm's ovum fresh chilli leaf, gently rinsed with sterile water and remove removal The dust of capsicum leaf surface, room temperature hang airing surface moisture, by clean capsicum leaf in 90 DEG C of hot water blanching 10s, other Auxiliary material aseptic water washing is clean, and dries surface moisture;
2) mixed raw material mashing with homogeneous: after taking pretreated capsicum leaf to mix with auxiliary material according to certain weight ratio, add water into The magma of capsicum leaf ferment fermentation is obtained after row mashing, homogeneous;
3) it mixes the preparation of ferment microbial inoculum inoculation liquid: tealeaves being added 90 according to w/v 1:50-200 (w/v, g/ml) 20min is extracted in DEG C -100 DEG C of hot water, filters to take millet paste;Millet paste pours into sterilized container, according still further to w/v 5% ~20% (wt%) be added table sugar, stir sufficiently after inoculation mixing ferment microbial inoculum, sealed with gauze, 28-35 DEG C with After 180rpm shaken cultivation 2-3d, plastic covering cloth is sealed on gauze, in 28-35 DEG C of stationary culture 3-5d;
4) capsicum leaf magma ferment ferments: the capsicum leaf and auxiliary material magma 20~50% (w/v, similarly hereinafter) of homogeneous;It is edible Sugared 5-20% mixes ferment microbial inoculum inoculation liquid 10%~30%, and surplus sterile purified water is sealed after mixing evenly with gauze, After 28-35 DEG C with 180rpm shaken cultivation 2-5d, plastic covering cloth is sealed on gauze, in 28-35 DEG C of stationary culture 5-30d;
5) allotment of capsicum leaf enzyme beverage: the filtering of capsicum leaf ferment tunning takes the ferment fermentation liquid of clear, then The fumet of 0.05-0.2% sweetener, 5% honey, 0.1~0.2% pectin, 0.01%-0.05% is added, after mixing evenly It stands more than for 24 hours, pours into clear glass bottle after then filtering with 0.2-0.45 μm of film, obtain capsicum leaf extract enzyme beverage.
3. a kind of production method of capsicum leaf enzyme beverage according to claim 2, it is characterised in that the accessory package Include one or more of mushroom, lily, pineapple, pawpaw.
4. a kind of production method of capsicum leaf enzyme beverage according to claim 2, it is characterised in that the mixing is former The weight of capsicum leaf accounts for 50-80% in material and the weight of auxiliary material accounts for 5~20%.
5. a kind of production method of capsicum leaf enzyme beverage according to claim 2, it is characterised in that the table sugar Mixture including one or more of white granulated sugar, rock sugar, brown sugar, soft white sugar, honey, concentrated sugarcane juice.
6. a kind of production method of capsicum leaf enzyme beverage according to claim 2, it is characterised in that the mixing ferment Plain microbial inoculum is one of independent or compounding aspergillus oryzae, lactic acid bacteria, saccharomycete or several using fermented tea as core microbial inoculum.
7. a kind of production method of capsicum leaf enzyme beverage according to claim 2, it is characterised in that the fermented tea Mother liquor be commercially available fermented tea, lactic acid bacteria be one or more of lactobacillus plantarum, leuconostoc pseudomesenteroides, lactobacillus acidophilus, Saccharomycete is one of saccharomyces cerevisiae, Hansenula anomala and Bayer Zygosaccharomyces or a variety of.
8. a kind of production method of capsicum leaf enzyme beverage according to claim 2, it is characterised in that the sweetener It is the composition of one or both of acesulfame potassium, stevioside.
9. a kind of production method of capsicum leaf enzyme beverage according to claim 2, it is characterised in that the fumet It is one or more of compositions of ethylmaltol, vanillic aldehyde, essence for food.
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