CN109463538A - A kind of preparation method of milk cow small molecule feed peptide - Google Patents
A kind of preparation method of milk cow small molecule feed peptide Download PDFInfo
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- CN109463538A CN109463538A CN201811619806.3A CN201811619806A CN109463538A CN 109463538 A CN109463538 A CN 109463538A CN 201811619806 A CN201811619806 A CN 201811619806A CN 109463538 A CN109463538 A CN 109463538A
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Abstract
The present invention relates to ox feed additive deep process technology field more particularly to a kind of preparation methods of milk cow small molecule feed peptide.The preparation method respectively obtains soybean meal extract and wheat embryo dregs of rice extract the following steps are included: digested respectively to Soybean Meal, the wheat embryo dregs of rice after boiling with lipase, amylase and neutral proteinase, then isolate and purify, dry；Radix Astragali and honeysuckle are extracted respectively, obtain Astragalus Root P.E and Honegsukle flower P.E；It is chelated after above four kinds of extracts are mixed under special ratios, is drying to obtain milk cow small molecule feed peptide.The problem of small molecule feed peptide being prepared is highly-safe, molecular weight product is small, milk cow eating effect is good, which is free of any antibiotic, avoids milk cow drug resistance, Residue of Antibiotics in Milk.
Description
Technical field
The present invention relates to ox feed additive deep process technology field more particularly to a kind of systems of milk cow small molecule feed peptide
Preparation Method.
Background technique
With the rapid development of China's milk industries, the continuous expansion of raising dairy cattle scale, producer is good for milk cow
The production performances such as health situation and the output of milk, butterfat percnetage and milk quality are also increasingly paid attention to, requirement of the consumer to dairy produce
Also higher and higher.And mastadenitis of cow is commonplace, can reduce the output of milk and milk quality, increases body cell quantity in milk
And bacterial micro-organism.Therefore, raiser reaches usually using cow feed additive improves the output of milk, improves milk composition and subtract
Few cow producing milk stress and other effects.The common additive of milk cattle cultivating has at this stage: nonprotein nitrogen, complex enzyme formulation, lactic acid bacteria
Preparation etc..Nonprotein nitrogen preparation mainly has urea and sulfate of ammoniac, can increase milk yield and dairy fat content；Complex enzyme formulation can be significant
Improve the fermentation quality of rice straw silage；Lactobacillus preparation can be improved corn silage lactic acid content and reduce acetic acid content, hence it is evident that
Improve Silage Quality.But most additives are probiotics fermention, but unknown to viral invalid or effect to milk cow intestinal beneficial
It is aobvious.Someone adds the antibiotic of low dosage to prevent and treat mastadenitis of cow, and the antibiotic of this low dosage can destroy in natural environment
The ecological balance between microorganism (including pathogenic microorganism) can also make milk cow generate drug resistance.And antibiotic has in milk
Residual, will seriously affect human health.
Summary of the invention
The problem unobvious for existing feed peptide addition effect, antibiotic is unfavorable to milk cow and human health, this hair
It is bright that a kind of preparation method of milk cow small molecule feed peptide is provided.
To achieve the above object of the invention, the embodiment of the present invention adopts the technical scheme that
A kind of preparation method of milk cow small molecule feed peptide, specifically includes the following steps:
Step a, water is added into Soybean Meal, boils 7~8h, with the fat for being equivalent to the Soybean Meal weight 1.5~2%
Enzyme, 1~1.5% amylase and 1~1.5% neutral proteinase digest the Soybean Meal after boiling, and are separated by solid-liquid separation, by institute
Liquid is obtained to be purified, it is dry, obtain soybean meal extract；
Step b, water is added into the wheat embryo dregs of rice, boils 7~8h, with being equivalent to the wheat embryo dregs of rice weight 1.5~2%
Lipase, 1~1.5% amylase and 1~1.5% neutral proteinase the wheat embryo dregs of rice after boiling are digested, Gu
Liquid separation, gained liquid is purified, dry, obtains wheat embryo dregs of rice extract；
Step c, Radix Astragali is extracted with water, it is dry, obtain Astragalus Root P.E；
Step d, honeysuckle 20~35%v/v ethyl alcohol is extracted, it is dry, obtain Honegsukle flower P.E；
Step e, 85.5~88.5 parts of the soybean meal extract, 0.5~1.5 part of wheat embryo dregs of rice extract, Huang are weighed
4.4~5.4 parts and 6.6~7.6 parts of Honegsukle flower P.E of stilbene extract, 150~200 parts of water are added and carry out chelatropic reaction, will
To chelating liquid be dried to get.
Using cooking method to Soybean Meal, the wheat embryo dregs of rice in the preparation method of milk cow small molecule feed peptide provided by the present invention
Pre-treatment is carried out, the organization softening of Soybean Meal and the wheat embryo dregs of rice can be made, be conducive to the leaching of effective component, improve polypeptide
Yield.The enzymatic hydrolysis of Soybean Meal and the wheat embryo dregs of rice is digested jointly using lipase, amylase and neutral proteinase, fat
Enzyme has decomposed the fat in Soybean Meal and the wheat embryo dregs of rice, and protein component is allowed to isolate in Soybean Meal and the wheat embryo dregs of rice
Come, then the protein separated is digested to obtain soybean peptide and wheat germ peptide by neutral proteinase, to improve
The recovery rate of peptide is extracted from Soybean Meal and the wheat embryo dregs of rice, that is, extract from the Soybean Meal and the wheat embryo dregs of rice of Unit Weight
More soybean peptides and wheat germ peptide out；Meanwhile lipase reduces the fat constituent in enzymolysis liquid, amylase can decompose shallow lake
Meal component, the two collective effect make the reduction of enzymolysis liquid viscosity, it is easier to filter, make more soybean peptides and wheat germ peptide
Into subsequent processing, be conducive to the yield for improving soybean peptide and wheat germ peptide, that is, soybean peptide and wheat embryo in finished product
Peptide accounts for the ratio of the soybean peptide and wheat germ peptide that extract in Soybean Meal and the wheat embryo dregs of rice.
Preferred quality than in range by soybean meal extract, wheat embryo dregs of rice extract, Astragalus Root P.E and gold and silver
Flower extract is chelated, and the soybean peptide and wheat germ peptide and chlorogenic acid, Astragaloside IV, wheat flavones etc. of small molecule can be made
Active material and vitamin, minerals, metal nutrient combine, and are conducive to quickly being absorbed and utilized for body, make gained
Product, which reaches, preferably improves milk cow body immunity, the output of milk and fresh milk protein, lactose and fat content, reduces fresh milk
Body cell and microorganism index, the effect for reducing mammitis.
The small molecule feed peptide being prepared with the preparation method of milk cow small molecule feed peptide in the present invention is highly-safe, produces
Product molecular weight is small, milk cow eating effect is good, which is free of any antibiotic, avoids milk cow drug resistance, antibiosis in milk
The problem of element residual.
Preferably, the weight of water described in step a and/or step b is 6~8 times of the Soybean Meal weight.Enough water
Amount the effective component of Soybean Meal, the wheat embryo dregs of rice can be made to be leached as much as possible during boiling, without regard to
Water is excessive, increases the subsequent concentration time, reduces production efficiency.
Preferably, the temperature boiled in step a and/or step b is 90~95 DEG C.The temperature can promote Soybean Meal, wheat
The leaching of bud dregs of rice effective component, the too low then decreased effectiveness of temperature, temperature is excessively high, can destroy part effective component.
Preferably, lipase described in step a and/or step b is Rhizopus oryzae lipase, and the amylase is aspergillus niger hair
Amylase obtained by ferment, the neutral proteinase are aspergillus oryzae protease.Rhizopus oryzae lipase has the single-minded of height for 1,3 positions
Property, and conversion ratio with higher and stability, it can effectively reduce fat.Acidproof alphalise starch can be obtained in fermentation of Aspergillus niger
Enzyme can act on Soybean Meal, the starch component in the wheat embryo dregs of rice, generate the reduced sugars such as maltose, maltotriose, dextrin, increase
Add product sweet taste, moreover it is possible to viscosity is reduced, so that enzyme digestion reaction sufficiently efficiently carries out.Aspergillus oryzae protease has wider
PH tolerance range and very strong protease synthesis capability, can high temperature resistant, stability is good, and can generate peculiar taste, makes product
Preferably meet requirement of the feed addictive to mouthfeel.
Preferably, the condition digested in step a and/or step b are as follows: 3~4h of stirring enzymatic hydrolysis under the conditions of 55~58 DEG C, so
After be warming up to 90~95 DEG C, keep 10~15min enzyme deactivation.Optimization of the operating method through excess temperature, enzymolysis time, when making enzymatic hydrolysis
Between shorten, efficiency improves, both hydrolyzed Soybean Meal, the substance that the fat in the wheat embryo dregs of rice, starch and indigestibility absorb, simultaneously
Also guarantee that active material is not destroyed.
Preferably, the operation of separation of solid and liquid described in step a and/or step b is to filter after being centrifuged, centrifugal rotational speed 3400
~3800r/min, to remove fat and insoluble matter in enzymolysis liquid.As preferred embodiment, which can be used sleeping
Gong centrifuge is more suitable for the separation of solid and liquid of thick liquid.
Purifying described in step a and/or step b carries out in three-phase centrifuge.
Preferably, the operation that the Radix Astragali is extracted in step c is to be added after being crushed Radix Astragali and be equivalent to the Radix Astragali
5~7 times of water of weight and 1.5~2.5% papain, and with salt acid for adjusting pH to 5.0~5.5, refluxing extraction 1.5~
2h is filtered, and is concentrated, dry, obtains Astragalus Root P.E.Papain can discharge active polysaccharide in cell wall, in conjunction with the pH ring
It border and uses water as Extraction solvent, the extraction of the ingredients such as astragalus polyose, Astragaloside IV can be promoted, and be hydrolyzed to astragalus polyose
Oligosaccharide makes milk cow be easier to be absorbed and utilized.Extracting resulting Astragalus Root P.E with the operation can be with the work in other raw materials
Property ingredient play preferably synergistic effect, strengthen Radix Astragali inducing diuresis to remove edema, antibacterial effect.
Preferably, the operation that the honeysuckle is extracted in step d is to be added after crushing honeysuckle and be equivalent to the gold and silver
Flower 6~8 times of 20~35%v/v ethyl alcohol of weight and 1.5~2.5% cellulases, and with salt acid for adjusting pH to 3.5~4, reflux mentions
1~1.5h is taken, is filtered, is concentrated, it is dry, obtain Honegsukle flower P.E.Cellulase can make honeysuckle cell wall rupture, in conjunction with this
PH environment and 20~35%v/v ethyl alcohol is used to be conducive to the extractions of the effective components such as Chlorogenic Acid of Flos Lonicerae as Extraction solvent,
The active constituent in Honegsukle flower P.E and other raw materials is set to play preferably synergistic effect, enhancing honeysuckle is clearing heat and detoxicating, disappears
It is swollen dispel sore the effect of.
Preferably, in step e chelatropic reaction concrete operations are as follows: 45~50 DEG C are warming up to, with the speed of 36~45r/min
Stir 30~45min.The chelating condition can be such that chelatropic reaction quickly and efficiently carries out.
Preferably, dry to be spray-dried described in step e, drying temperature is 45~55 DEG C.Drying temperature is too low, does
Dry speed is slow, low efficiency, and drying temperature is excessively high then to have a degree of destruction to the effective active composition in product.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this
Invention is further elaborated.It should be appreciated that specific embodiment described herein is used only for explaining the present invention, not
For limiting the present invention.
Embodiment 1
A kind of preparation method of milk cow small molecule feed peptide is present embodiments provided, specifically includes the following steps:
Step a, 6L water is added into 1kg Soybean Meal, boils 7h under the conditions of 90 DEG C, is cooled to 56 DEG C, 15g Rhizopus oryzae is added
Lipase, 10g amylase (obtained by fermentation of Aspergillus niger) and 10g aspergillus oryzae protease digest 3h in 55 DEG C of stirrings.After the completion of enzymatic hydrolysis,
90 DEG C are warming up to, 10min enzyme deactivation is kept.It is centrifuged under the revolving speed of 3600r/min with decanter centrifuge, is filtered to remove solid content,
Three-phase centrifuge centrifugal purification under the revolving speed of 18000r/min is used again, it is dry, obtain soybean meal extract.
Step b, 6L water is added into the 1kg wheat embryo dregs of rice, boils 7h under the conditions of 90 DEG C, is cooled to 55 DEG C, is added 15g meters
Rizolipase, 10g amylase (obtained by fermentation of Aspergillus niger) and 10g aspergillus oryzae protease digest 3h in 55 DEG C of stirrings.It has digested
Cheng Hou is warming up to 90 DEG C, keeps 10min enzyme deactivation.It is centrifuged, is filtered to remove solid under the revolving speed of 3600r/min with decanter centrifuge
Shape object, then centrifugal purification, drying obtain wheat embryo dregs of rice extract under the revolving speed of 18000r/min with three-phase centrifuge.
Step c, 5L water and 15g papain are added after being crushed 1kg Radix Astragali, and with salt acid for adjusting pH to 5.0,
Refluxing extraction 1.5h is filtered, and is concentrated, dry, obtains Astragalus Root P.E.
Step d, will 1kg honeysuckle crush after be added 6L 20%v/v ethyl alcohol and 15g cellulase, and with salt acid for adjusting pH
It to 3.5, refluxing extraction 1h, filters, is concentrated, it is dry, obtain Honegsukle flower P.E.
Step e, 85.5 parts of the soybean meal extract, 0.5 part of wheat embryo dregs of rice extract, Astragalus Root P.E 4.4 are weighed
Part and 6.6 parts of Honegsukle flower P.E, 150 parts of water are added, are warming up to 45 DEG C, are chelated with the speed of 36r/min stirring 45min
Gained chelating liquid is concentrated with dual-effect concentrator for reaction, then small to get milk cow is arrived in 45~55 DEG C of at a temperature of spray drying
Molecule feed peptide.
Products obtained therefrom is used as cow feed additive, and milk cow forage additive amount per ton is 500g.
Embodiment 2
A kind of preparation method of milk cow small molecule feed peptide is present embodiments provided, specifically includes the following steps:
Step a, 75L water is added into 10kg Soybean Meal, boils 7.5h under the conditions of 92 DEG C, is cooled to 57 DEG C, 175g is added
Rhizopus oryzae lipase, 125g amylase (obtained by fermentation of Aspergillus niger) and 125g aspergillus oryzae protease digest 3.5h in 57 DEG C of stirrings.
After the completion of enzymatic hydrolysis, 92 DEG C are warming up to, keeps 12min enzyme deactivation.It is centrifuged, is filtered under the revolving speed of 3400r/min with decanter centrifuge
Solid content is removed, then centrifugal purification, drying obtain soybean meal extract under the revolving speed of 18000r/min with three-phase centrifuge.
Step b, 75L water is added into the 10kg wheat embryo dregs of rice, boils 7.5h under the conditions of 92 DEG C, is cooled to 57 DEG C, is added
175g Rhizopus oryzae lipase, 125g amylase (obtained by fermentation of Aspergillus niger) and 125g aspergillus oryzae protease are digested in 57 DEG C of stirrings
3.5h.After the completion of enzymatic hydrolysis, 92 DEG C are warming up to, keeps 12min enzyme deactivation.It is centrifuged under the revolving speed of 3400r/min with decanter centrifuge,
It is filtered to remove solid content, then centrifugal purification, drying obtain the wheat embryo dregs of rice and mention under the revolving speed of 18000r/min with three-phase centrifuge
Take object.
Step c, 60L water and 200g papain are added after being crushed 10kg Radix Astragali, and extremely with salt acid for adjusting pH
5.2, refluxing extraction 1.5h are filtered, and are concentrated, dry, obtain Astragalus Root P.E.
Step d, 7L 25%v/v ethyl alcohol and 200g cellulase are added after crushing 10kg honeysuckle, and is adjusted with hydrochloric acid
PH to 3.8, refluxing extraction 1h are filtered, and are concentrated, dry, obtain Honegsukle flower P.E.
Step e, weigh 870 parts of the soybean meal extract, 10 parts of wheat embryo dregs of rice extract, 50 parts of Astragalus Root P.E and
70 parts of Honegsukle flower P.E, 1800 parts of water are added, are warming up to 47 DEG C, chelate with the speed stirring 40min of 40r/min anti-
Answer, gained chelating liquid be concentrated with dual-effect concentrator, then 45~55 DEG C at a temperature of be spray-dried to get arriving small point of milk cow
Sub- feed peptide.
Products obtained therefrom is used as cow feed additive, and milk cow forage additive amount per ton is 1kg.
Embodiment 3
A kind of preparation method of milk cow small molecule feed peptide is present embodiments provided, specifically includes the following steps:
Step a, 4000kg water is added into 500kg Soybean Meal, boils 8h under the conditions of 95 DEG C, is cooled to 58 DEG C, is added
10kg Rhizopus oryzae lipase, 7.5kg amylase (obtained by fermentation of Aspergillus niger) and 7.5kg aspergillus oryzae protease are digested in 58 DEG C of stirrings
4h.After the completion of enzymatic hydrolysis, 95 DEG C are warming up to, keeps 15min enzyme deactivation.It is centrifuged under the revolving speed of 3800r/min with decanter centrifuge, mistake
Solid content is filtered out, then centrifugal purification, drying obtain soybean meal extract under the revolving speed of 18000r/min with three-phase centrifuge.
Step b, 4000kg water is added into the 500kg wheat embryo dregs of rice, boils 8h under the conditions of 95 DEG C, is cooled to 58 DEG C, adds
Enter 10kg Rhizopus oryzae lipase, 7.5kg amylase (obtained by fermentation of Aspergillus niger) and 7.5kg aspergillus oryzae protease in 58 DEG C of stirring enzymes
Solve 4h.After the completion of enzymatic hydrolysis, 95 DEG C are warming up to, keeps 15min enzyme deactivation.It is centrifuged under the revolving speed of 3800r/min with decanter centrifuge,
It is filtered to remove solid content, then centrifugal purification, drying obtain the wheat embryo dregs of rice and mention under the revolving speed of 18000r/min with three-phase centrifuge
Take object.
Step c, 3500L water and 12.5kg papain are added after being crushed 500kg Radix Astragali, and is adjusted with hydrochloric acid
PH to 5.5, refluxing extraction 2h are filtered, and are concentrated, dry, obtain Astragalus Root P.E.
Step d, 4000L 35%v/v ethyl alcohol and 12.5kg cellulase are added after crushing 500kg honeysuckle, and uses salt
Acid for adjusting pH is filtered, is concentrated, drying obtains Honegsukle flower P.E to 4.0, refluxing extraction 1.5h.
Step e, 88.5 parts of the soybean meal extract, 1.5 parts of wheat embryo dregs of rice extract, Astragalus Root P.E 5.4 are weighed
Part and 7.6 parts of Honegsukle flower P.E, 200 parts of water are added, are warming up to 50 DEG C, are chelated with the speed of 45r/min stirring 30min
Gained chelating liquid is concentrated with dual-effect concentrator for reaction, then small to get milk cow is arrived in 45~55 DEG C of at a temperature of spray drying
Molecule feed peptide.
Products obtained therefrom is used as cow feed additive, and milk cow forage additive amount per ton is 1.5kg.
Comparative example
This comparative example provides a kind of preparation method of milk cow small molecule feed peptide, has adjusted on the basis of embodiment each
The proportion of ingredient.Specifically includes the following steps:
Step a, 6kg water is added into 1kg Soybean Meal, boils 7h under the conditions of 90 DEG C, is cooled to 56 DEG C, 15g meters of roots are added
Mould lipase, 10g amylase (obtained by fermentation of Aspergillus niger) and 10g aspergillus oryzae protease digest 3.5h in 55 DEG C of stirrings.It has digested
Cheng Hou is warming up to 92 DEG C, keeps 12min enzyme deactivation.It is centrifuged, is filtered to remove solid under the revolving speed of 3600r/min with decanter centrifuge
Shape object, then centrifugal purification, drying obtain soybean meal extract under the revolving speed of 18000r/min with three-phase centrifuge.
Step b, 6kg water is added into the 1kg wheat embryo dregs of rice, boils 7h under the conditions of 90 DEG C, is cooled to 56 DEG C, 15g is added
Rhizopus oryzae lipase, 10g amylase (obtained by fermentation of Aspergillus niger) and 10g aspergillus oryzae protease digest 3h in 55 DEG C of stirrings.Enzymatic hydrolysis
After the completion, 90 DEG C are warming up to, 10min enzyme deactivation is kept.It is centrifuged, is filtered to remove under the revolving speed of 3600r/min with decanter centrifuge
Solid content, then centrifugal purification, drying obtain wheat embryo dregs of rice extract under the revolving speed of 18000r/min with three-phase centrifuge.
Step c, 5kg water and 15g papain are added after being crushed 1kg Radix Astragali, and extremely with salt acid for adjusting pH
5.0, refluxing extraction 1.5h are filtered, and are concentrated, dry, obtain Astragalus Root P.E.
Step d, 6kg 20%v/v ethyl alcohol and 15g cellulase are added after crushing 1kg honeysuckle, and is adjusted with hydrochloric acid
PH to 3.5, refluxing extraction 1 filter, and are concentrated, dry, obtain Honegsukle flower P.E.
Step e, 610 parts of the soybean meal extract, 30 parts of wheat embryo dregs of rice extract, 150 parts of Astragalus Root P.E are weighed
With 210 parts of Honegsukle flower P.E, 1800 parts of water are added, are warming up to 47 DEG C, are chelated with the speed of 40r/min stirring 40min
Gained chelating liquid is concentrated with dual-effect concentrator for reaction, then small to get milk cow is arrived in 45~55 DEG C of at a temperature of spray drying
Molecule feed peptide.
Products obtained therefrom is used as cow feed additive, and milk cow forage additive amount per ton is 500g.
Effect example
To Examples 1 to 3 and comparative example products obtained therefrom carry out milk production of cow, fresh milk protein content, lactose content,
Fat content, fresh milk body cell and microorganism index analysis.
Experimental animal: the healthy cow for peak period of giving milk 20 is randomly divided into 5 groups, respectively blank group, example 1 group,
2 groups of embodiment, embodiment 3 combine comparative example group.
Test method: Examples 1 to 3 and comparative example products obtained therefrom are added in feed by the dosage of 1g/kg, often
Rule are fed 3 weeks, and protein content, cream in the average daily milk production and fresh milk for detecting every cow head daily at third week
Sugared content, fat content, fresh milk body cell and microorganism index, the results are shown in Table 1.
1 each group milk production of cow of table and milk matter are investigated
Examples 1 to 3 group and comparative example group milk cow compared to the blank group, P < 0.05；Embodiment each group and comparative example group phase
Than protein content, fresh milk body cell and microorganism indices P < 0.05 in average daily milk production and fresh milk.
As 1 test result of table as it can be seen that the product produced by preparation method provided by Examples 1 to 3 can be mentioned significantly
High milk cow body immunity, the output of milk and fresh milk protein, lactose and fat content, reduce fresh milk body cell and microorganism refers to
Number.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modification, equivalent replacement or improvement etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (10)
1. a kind of preparation method of milk cow small molecule feed peptide, which is characterized in that specifically includes the following steps:
Step a, water is added into Soybean Meal, boils 7~8h, be equivalent to the Soybean Meal weight 1.5~2% lipase, 1~
1.5% amylase and 1~1.5% neutral proteinase digest the Soybean Meal after boiling, and are separated by solid-liquid separation, by gained liquid
It is purified, it is dry, obtain soybean meal extract；
Step b, water is added into the wheat embryo dregs of rice, boils 7~8h, with the rouge for being equivalent to the wheat embryo dregs of rice weight 1.5~2%
Fat enzyme, 1~1.5% amylase and 1~1.5% neutral proteinase digest the wheat embryo dregs of rice after boiling, solid-liquid point
From, gained liquid is purified, it is dry, obtain wheat embryo dregs of rice extract；
Step c, Radix Astragali is extracted with water, it is dry, obtain Astragalus Root P.E；
Step d, honeysuckle 20~35%v/v ethyl alcohol is extracted, it is dry, obtain Honegsukle flower P.E；
Step e, 85.5~88.5 parts of the soybean meal extract, 0.5~1.5 part of wheat embryo dregs of rice extract, Radix Astragali is weighed to mention
4.4~5.4 parts and 6.6~7.6 parts of Honegsukle flower P.E of object are taken, 150~200 parts of water are added and carry out chelatropic reaction, by what is obtained
Chelating liquid be dried to get.
2. the preparation method of milk cow small molecule feed peptide according to claim 1, which is characterized in that step a and/or step
The weight of water described in b is 6~8 times of the Soybean Meal weight.
3. the preparation method of milk cow small molecule feed peptide according to claim 2, which is characterized in that step a and/or step
The temperature boiled in b is 90~95 DEG C.
4. the preparation method of milk cow small molecule feed peptide according to claim 1, which is characterized in that step a and/or step
Lipase described in b is Rhizopus oryzae lipase, and the amylase is amylase obtained by fermentation of Aspergillus niger, and the neutral proteinase is
Aspergillus oryzae protease.
5. the preparation method of milk cow small molecule feed peptide according to claim 4, which is characterized in that step a and/or step
The condition digested in b are as follows: 3~4h of stirring enzymatic hydrolysis under the conditions of 55~58 DEG C then heats to 90~95 DEG C, keep 10~
15min enzyme deactivation.
6. the preparation method of milk cow small molecule feed peptide according to claim 1, which is characterized in that step a and/or step
The operation of separation of solid and liquid described in b is to filter after being centrifuged, and centrifugal rotational speed is 3400~3800r/min；And/or
Purifying described in step a and/or step b carries out in three-phase centrifuge.
7. the preparation method of milk cow small molecule feed peptide according to claim 1, which is characterized in that extract institute in step c
The operation for stating Radix Astragali is that the wood for being equivalent to 5~7 times of water of the astragalus weight and 1.5~2.5% is added after being crushed Radix Astragali
Melon protease, and with salt acid for adjusting pH to 5.0~5.5,1.5~2h of refluxing extraction is filtered, and is concentrated, and it is dry, obtain astragalus extraction
Object.
8. the preparation method of milk cow small molecule feed peptide according to claim 1, which is characterized in that extract institute in step d
The operation for stating honeysuckle is to be added after crushing honeysuckle and be equivalent to 6~8 times of 20~35%v/v ethyl alcohol of the honeysuckle weight
With 1.5~2.5% cellulases, and with salt acid for adjusting pH to 3.5~4,1~1.5h of refluxing extraction is filtered, and is concentrated, dry, is obtained
Honegsukle flower P.E.
9. the preparation method of milk cow small molecule feed peptide according to claim 1, which is characterized in that chelated in step e anti-
The concrete operations answered are as follows: be warming up to 45~50 DEG C, 30~45min is stirred with the speed of 36~45r/min.
10. the preparation method of milk cow small molecule feed peptide according to claim 1, which is characterized in that done described in step e
Dry is spray drying, and drying temperature is 45~55 DEG C.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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