CN104247893A - Method for preparing biological fermentation peptide for increasing dairy cattle milk by combination of enzymolysis method and fermentation method - Google Patents
Method for preparing biological fermentation peptide for increasing dairy cattle milk by combination of enzymolysis method and fermentation method Download PDFInfo
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Abstract
The invention discloses a method for preparing a biological fermentation peptide for increasing dairy cattle milk by combination of an enzymolysis method and a fermentation method. According to the method, based on the biological mechanism of dairy cattle in producing milk, raw materials such as bean pulp and mixed mea together with milk increasing agents such as traditional Chinese herbal medicines, sodium selenite and medical stone are adopted, and a process that the enzymolysis of externally added alkaline protease and the microorganism biological fermentation are combined is adopted, so as to prepare the biological fermentation peptide which can increase dairy cattle milk, and is rich in functional nutrient components such as polypeptide, small peptide, amino acid, organic acid, probiotics and active components of traditional Chinese herbal medicines. The content of polypeptide in the product is greater than or equal to 60%, the problems that the nutrition digestion rate and the absorption rate are low, the milk yield is low, and the anti-disease property of bodies is poor in the dairy cattle breeding are well solved when the peptide is used in feed, the milk production property of dairy cattle is improved, and meanwhile due to addition of products such as the traditional Chinese herbal medicines, sodium selenite and medical stone, the lactation property of the dairy cattle is further improved, and the milk increase effect is better.
Description
Technical field
The present invention relates to a kind of method of combining enzymatic isolation method and fermentation method and preparing fodder for increasing milk biofermentation peptide, belong to feed additive field.
Background technology
Protein raw material is the basis of feed industrial development, and the in short supply and price of China's protein raw material is surging has become one of bottleneck of restriction animal husbandry development.Raising along with people's living standard at present and the attention to quality of life, increasing to the demand of meat, egg, milk, aquatic products, Fodder and food is produced and is faced a severe test, feed protein resource will be day by day deficient, therefore the transformation efficiency of grain to livestock products and the utilization rate of feed is improved, expand feed resource, exploitation unconventional water resources is extremely urgent.And the Critical policies alleviated the problems is exactly for existing protein sources, improve its utilization rate, excavate the utilized nutritional labeling of feed as much as possible, fundamentally alleviate feedstuff situation in short supply.
China milk industry is through the fast development of more than ten years, and within 2013, China's cow population is more than 1,500 ten thousand.But it is low all the time to there is raise competence in China milk industry, milk production of cow is on the low side, average per unit area yield only has about 5400Kg, lactoprotein, butterfat percnetage are on the low side, and (lactoprotein is 2.95%-3.0%, butterfat percnetage 3.1%-3.2%) etc. problem, had a strong impact on the sound development of milk cow production and the supply of dietetic milk prod.Compared with China, the dairy industry developed country milk cow average milk production such as the U.S. have reached 9000-10000Kg, and lactoprotein is 3.2%-3.5%, and butterfat percnetage is 3.5%-4.0%.The high-quality roughage raw materials such as clover are lacked due to China, be main roughage with maize straw etc., and fine fodder too much (too much can not can cause the symptoms such as rumen ecology), therefore, present stage, yard feeding pattern caused the biological transformation function of cow rumen fermentable function, mammary gland lactation function and liver relative low, forage protein biological transformation ratio is low, Milk Production amount and dairy products quality lower, this has become the bottleneck of restriction China milk cow production development.
In recent years scientific research finds, animal body panning protein is after digestion, and non-principal absorbs with amino acid form, but absorbs with the form of peptide.In addition, some peptides can not only provide animal body to grow, grow desired nutritional material, also have different physiological roles simultaneously.Current Production by Enzymes functional polypeptide application is comparatively general, and combine enzymatic isolation method and fermentation method prepares fodder for increasing milk biofermentation peptide, particularly associating enzymatic isolation method and fermentation method prepare the assorted dregs of rice fodder for increasing milk biofermentation peptides such as sesame seed meal, peanut meal, Cottonseed Meal, rapeseed dregs, have no the report of commercial application.
Summary of the invention
The object of the invention is to solve the problem that milk production of cow and dairy products quality under the straw nutrition pattern of current China Cow-feeding are on the low side; to give milk biomechanism according to cow; utilize the puffed soybean raw material such as dregs of beans, the assorted dregs of rice; by associating enzymatic isolation method and fermentation method; prepare high-activity biological fermentation peptide; and be aided with the increasing milk agents such as Chinese herbal medicine, sodium selenite, medical stone, a kind of method preparing fodder for increasing milk biofermentation peptide being convenient to large-scale production is provided.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The invention provides a kind of method of combining enzymatic isolation method and fermentation method and preparing fodder for increasing milk biofermentation peptide, it is characterized in that: utilize the puffed soybean raw material such as dregs of beans, the assorted dregs of rice, be aided with the increasing milk agents such as Chinese herbal medicine, sodium selenite, medical stone, be rich in the fodder for increasing milk biofermentation peptide of the functional nutrient compositions such as polypeptide, little peptide, amino acid, organic acid, probio, Chinese herbal medicine active component by the ferment technique preparation that combines of the enzymolysis of exogenous interpolation alkali protease and microorganism biological.
Said herbal medicine mainly comprises: the seed of cowherb, Radix Angelicae Sinensis, the road stem pith of the rice-paper plant, Radix Glycyrrhizae, Radix Codonopsis.
The invention provides a kind of method of combining enzymatic isolation method and fermentation method and preparing fodder for increasing milk biofermentation peptide, it is characterized in that enzymatic hydrolysis and fermentation raw material composition mass fraction is: dregs of beans 75 ~ 85 parts, the assorted dregs of rice 12 ~ 18 parts, the seed of cowherb 0.5 ~ 1.5 part, Radix Angelicae Sinensis 0.5 ~ 1.5 part, the road stem pith of the rice-paper plant 0.5 ~ 1.5 part, 0.5 ~ 1.5 part, Radix Glycyrrhizae, Radix Codonopsis 0.5 ~ 1.5 part.
Described assorted dregs of rice class raw material is one or both in sesame seed meal, peanut meal, Cottonseed Meal, rapeseed dregs.
The invention provides a kind of method of combining enzymatic isolation method and fermentation method and preparing fodder for increasing milk biofermentation peptide, it is characterized in that being realized by following approach:
(1) co-grinding: enzymatic hydrolysis and fermentation raw material is mixed rear pulverizing in proportion, crosses 40 ~ 60 mesh sieves;
(2) enzymatic hydrolysis and fermentation culture medium: according to enzymatic hydrolysis and fermentation raw material 93 ~ 95%, molasses 2 ~ 4%, peptone 1 ~ 2%, NH
4cl 1.0%, KH
2pO4.3H
2o 0.5%, MgSO
4.7H
2the ratio of O 0.5% takes material, mixes, and adjustment material moisture is 15 ~ 20%;
(3) enzyme-added: according to the exogenous interpolation alkali protease of the ratio of enzymatic hydrolysis and fermentation material quality than 6 ~ 8%;
(4) saccharomyces cerevisiae activation: activate saccharomyces cerevisiae according to the ratio of 1:30 ~ 50 in Czapek's medium;
(5) inoculate: according to the inoculative proportion of 5 ~ 8%, saccharomyces cerevisiae is seeded to fermentation medium;
(6) mix: the fermentation medium after access saccharomyces cerevisiae and protease is mixed;
(7) solid fermentation: culture medium is moved into fermentation vat temperature controlled fermentation 32h ~ 48h, fermentation temperature controls at 35 ~ 38 DEG C;
(8) two-step fermentation culture medium: the culture medium after terminating to fermentation by saccharomyces cerevisiae adds 1 ~ 2% molasses, 0.1 ~ 0.5% acetic acid;
(9) actication of culture: mix by 1:30 ~ 50 weight ratio with 30 ~ 35 DEG C of warm water with bacillus subtilis, Bacillus acidi lactici, stirs until bacterial classification is dissolved in the water completely, activation 20 ~ 30min;
(10) inoculate: by inoculative proportion inoculation bacillus subtilis and the Bacillus acidi lactici of bacillus subtilis 2 ~ 3%, Bacillus acidi lactici 3 ~ 5%, mix;
(11) two-step fermentation: culture medium is moved into fermentation vat, temperature controlled fermentation 48 ~ 72h, fermentation temperature controls at 28 ~ 32 DEG C;
(12) low temperature drying: after fermentation ends, low temperature drying material is to moisture 10 ~ 12%;
(13) pulverize: the peptide that will ferment after fermentation ends is pulverized, cross 60 mesh sieves;
(14) composite: by sodium selenite and medical stone respectively in biofermentation peptide mass ratio 2 ~ 3%, 3 ~ 5% ratio add;
(15) mix: biofermentation peptide, sodium selenite, medical stone are mixed and obtain fodder for increasing milk biofermentation peptide feed addictive.
Above-mentioned two-step fermentation is prepared in the production technology of fodder for increasing milk biofermentation peptide, and fermentation materials is without the need to sterilizing.
Above-mentioned two-step fermentation is prepared in the production technology of fodder for increasing milk biofermentation peptide, when fermentation raw material temperature rises in step (7) and step (11), can consider to pass into and filter cold wind control fermentation temperature.
The invention provides a kind of method of combining enzymatic isolation method and fermentation method and preparing fodder for increasing milk biofermentation peptide, described fermented type Chinese herb medicine additive for milk cow forage product use amount in dairy cow diet is 200 ~ 500g; Adopt feed addictive of the present invention to feed, milk production of cow generally improves 2.5-4.2Kg, and butterfat percnetage and lactoprotein improve 8-10%.
The invention provides a kind of method of combining enzymatic isolation method and fermentation method and preparing fodder for increasing milk biofermentation peptide, by alkali protease enzymolysis and fermentable effect, in fermentation peptide product more than content of peptides >=70%, be rich in multiple digestive ferment and probio, degrade most of antigen protein in the grouts dregs of rice, compound sugar, the ANFs such as phytic acid and tannin, and give a certain amount of organic acid and flavour, be applied in feed that well to solve milk cattle cultivating Middle nutrition digestibility low, rate of giving milk is low, the problems such as body resistance against diseases is weak, improve cow producing milk performance, compound of the present invention with the addition of the products such as Chinese herbal medicine, sodium selenite, medical stone simultaneously, further increases the increasing breast effect of fodder for increasing milk biofermentation peptide product, has compared with similar products and better increase newborn usefulness.
Detailed description of the invention
embodiment 1:
Combine the method that enzymatic isolation method and fermentation method prepare fodder for increasing milk biofermentation peptide, concrete preparation process is as follows:
(1) co-grinding: by raw material in following ratio mixing dregs of beans 75 parts, peanut meal 9 parts, sesame seed meal 9 parts, the seed of cowherb 1.5 parts, Radix Angelicae Sinensis 1.5 parts, the road stem pith of the rice-paper plant 1.5 parts, 1.5 parts, Radix Glycyrrhizae, Radix Codonopsis 1 part; Pulverize after mixing, cross 40 ~ 60 mesh sieves;
(2) enzymatic hydrolysis and fermentation culture medium: according to enzymatic hydrolysis and fermentation raw material 93%, molasses 4%, peptone 1%, NH
4cl 1.0%, KH
2pO4.3H
2o 0.5%, MgSO
4.7H
2the ratio of O 0.5% takes material, mixes, and adjustment material moisture is 15 ~ 20%;
(3) enzyme-added: according to the exogenous interpolation alkali protease of the ratio of enzymatic hydrolysis and fermentation material quality than 6%;
(4) saccharomyces cerevisiae activation: activate saccharomyces cerevisiae according to the ratio of 1:30 in Czapek's medium;
(5) inoculate: according to the inoculative proportion of 8%, saccharomyces cerevisiae is seeded to fermentation medium;
(6) mix: the fermentation medium after access saccharomyces cerevisiae and protease is mixed;
(7) solid fermentation: culture medium is moved into fermentation vat temperature controlled fermentation 32h ~ 48h, fermentation temperature controls at 35 ~ 38 DEG C;
(8) two-step fermentation culture medium: the culture medium after fermentation by saccharomyces cerevisiae terminates adds 2% molasses, 0.5% acetic acid;
(9) actication of culture: mix by 1:30 ~ 50 weight ratio with 30 ~ 35 DEG C of warm water with bacillus subtilis, Bacillus acidi lactici, stirs until bacterial classification is dissolved in the water completely, activation 20 ~ 30min;
(10) inoculate: by inoculative proportion inoculation bacillus subtilis and the Bacillus acidi lactici of bacillus subtilis 3%, Bacillus acidi lactici 5%, mix;
(11) two-step fermentation: culture medium is moved into fermentation vat, temperature controlled fermentation 48 ~ 72h, fermentation temperature controls at 28 ~ 32 DEG C;
(12) low temperature drying: after fermentation ends, low temperature drying material is to moisture 10 ~ 12%;
(13) pulverize: the peptide that will ferment after fermentation ends is pulverized, cross 60 mesh sieves;
(14) composite: by sodium selenite and medical stone respectively in biofermentation peptide mass ratio 3%, 3% ratio add;
(15) mix: biofermentation peptide, sodium selenite, medical stone are mixed and obtain fodder for increasing milk biofermentation peptide feed addictive.
embodiment 2:
Combine the method that enzymatic isolation method and fermentation method prepare fodder for increasing milk biofermentation peptide, concrete preparation process is as follows:
(1) co-grinding: by raw material in following ratio mixing dregs of beans 80 parts, the assorted dregs of rice 15 parts, the seed of cowherb 1 part, Radix Angelicae Sinensis 1 part, the road stem pith of the rice-paper plant 1,1 part, Radix Glycyrrhizae, Radix Codonopsis 1 part; Pulverize after mixing, cross 40 ~ 60 mesh sieves;
(2) enzymatic hydrolysis and fermentation culture medium: according to enzymatic hydrolysis and fermentation raw material 94%, molasses 2%, peptone 2%, NH
4cl 1.0%, KH
2pO4.3H
2o 0.5%, MgSO
4.7H
2the ratio of O 0.5% takes material, mixes, and adjustment material moisture is 15 ~ 20%;
(3) enzyme-added: according to the exogenous interpolation alkali protease of the ratio of enzymatic hydrolysis and fermentation material quality than 6%;
(4) saccharomyces cerevisiae activation: activate saccharomyces cerevisiae according to the ratio of 1:50 in Czapek's medium;
(5) inoculate: according to the inoculative proportion of 5 ~ 8%, saccharomyces cerevisiae is seeded to fermentation medium;
(6) mix: the fermentation medium after access saccharomyces cerevisiae and protease is mixed;
(7) solid fermentation: culture medium is moved into fermentation vat temperature controlled fermentation 32h ~ 48h, fermentation temperature controls at 35 ~ 38 DEG C;
(8) two-step fermentation culture medium: the culture medium after fermentation by saccharomyces cerevisiae terminates adds 1% molasses, 0.1% acetic acid;
(9) actication of culture: mix by 1:40 weight ratio with 30 ~ 35 DEG C of warm water with bacillus subtilis, Bacillus acidi lactici, stirs until bacterial classification is dissolved in the water completely, activation 20 ~ 30min;
(10) inoculate: by inoculative proportion inoculation bacillus subtilis and the Bacillus acidi lactici of bacillus subtilis 2.5%, Bacillus acidi lactici 4%, mix;
(11) two-step fermentation: culture medium is moved into fermentation vat, temperature controlled fermentation 48 ~ 72h, fermentation temperature controls at 28 ~ 32 DEG C;
(12) low temperature drying: after fermentation ends, low temperature drying material is to moisture 10 ~ 12%;
(13) pulverize: the peptide that will ferment after fermentation ends is pulverized, cross 60 mesh sieves;
(14) composite: by sodium selenite and medical stone respectively in biofermentation peptide mass ratio 2.5%, 4% ratio add;
(15) mix: biofermentation peptide, sodium selenite, medical stone are mixed and obtain fodder for increasing milk biofermentation peptide feed addictive.
embodiment 3:
Combine the method that enzymatic isolation method and fermentation method prepare fodder for increasing milk biofermentation peptide, concrete preparation process is as follows:
(1) co-grinding: by raw material in following ratio mixing dregs of beans 85 parts, Cottonseed Meal 4 parts, rapeseed dregs 8 parts, the seed of cowherb 0.6 part, Radix Angelicae Sinensis 0.6 part, the road stem pith of the rice-paper plant 0.6 part, 0.6 part, Radix Glycyrrhizae, Radix Codonopsis 0.6 part; Pulverize after mixing, cross 40 ~ 60 mesh sieves;
(2) enzymatic hydrolysis and fermentation culture medium: according to enzymatic hydrolysis and fermentation raw material 95%, molasses 2%, peptone 1%, NH
4cl 1.0%, KH
2pO4.3H
2o 0.5%, MgSO
4.7H
2the ratio of O 0.5% takes material, mixes, and adjustment material moisture is 15 ~ 20%;
(3) enzyme-added: according to the exogenous interpolation alkali protease of the ratio of enzymatic hydrolysis and fermentation material quality than 7%;
(4) saccharomyces cerevisiae activation: activate saccharomyces cerevisiae according to the ratio of 1:40 in Czapek's medium;
(5) inoculate: according to the inoculative proportion of 7%, saccharomyces cerevisiae is seeded to fermentation medium;
(6) mix: the fermentation medium after access saccharomyces cerevisiae and protease is mixed;
(7) solid fermentation: culture medium is moved into fermentation vat temperature controlled fermentation 32h ~ 48h, fermentation temperature controls at 35 ~ 38 DEG C;
(8) two-step fermentation culture medium: the culture medium after fermentation by saccharomyces cerevisiae terminates adds 1.5% molasses, 0.3% acetic acid;
(9) actication of culture: mix by 1:30 weight ratio with 30 ~ 35 DEG C of warm water with bacillus subtilis, Bacillus acidi lactici, stirs until bacterial classification is dissolved in the water completely, activation 20 ~ 30min;
(10) inoculate: by inoculative proportion inoculation bacillus subtilis and the Bacillus acidi lactici of bacillus subtilis 3%, Bacillus acidi lactici 5%, mix;
(11) two-step fermentation: culture medium is moved into fermentation vat, temperature controlled fermentation 48 ~ 72h, fermentation temperature controls at 28 ~ 32 DEG C;
(12) low temperature drying: after fermentation ends, low temperature drying material is to moisture 10 ~ 12%;
(13) pulverize: the peptide that will ferment after fermentation ends is pulverized, cross 60 mesh sieves;
(14) composite: by sodium selenite and medical stone respectively in biofermentation peptide mass ratio 2%, 5% ratio add;
(15) mix: biofermentation peptide, sodium selenite, medical stone are mixed and obtain fodder for increasing milk biofermentation peptide feed addictive.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (2)
1. combine enzymatic isolation method and fermentation method and prepare a fodder for increasing milk biofermentation peptide, it is characterized in that being realized by following approach:
(1) co-grinding: pulverize in after dregs of beans 75 ~ 85 parts, the assorted dregs of rice 12 ~ 18 parts, the seed of cowherb 0.5 ~ 1.5 part, Radix Angelicae Sinensis 0.5 ~ 1.5 part, the road stem pith of the rice-paper plant 0.5 ~ 1.5 part, 0.5 ~ 1.5 part, Radix Glycyrrhizae, Radix Codonopsis 0.5 ~ 1.5 part of ratio mixing, cross 40 ~ 60 mesh sieves;
(2) enzymatic hydrolysis and fermentation culture medium: according to enzymatic hydrolysis and fermentation raw material 93 ~ 95%, molasses 2 ~ 4%, peptone 1 ~ 2%, NH
4cl 1.0%, KH
2pO4.3H
2o 0.5%, MgSO
4.7H
2the ratio of O 0.5% takes material, mixes, and adjustment material moisture is 15 ~ 20%;
(3) enzyme-added: according to the exogenous interpolation alkali protease of the ratio of enzymatic hydrolysis and fermentation material quality than 6 ~ 8%;
(4) saccharomyces cerevisiae activation: activate saccharomyces cerevisiae according to the ratio of 1:30 ~ 50 in Czapek's medium;
(5) inoculate: according to the inoculative proportion of 5 ~ 8%, saccharomyces cerevisiae is seeded to fermentation medium;
(6) mix: the fermentation medium after access saccharomyces cerevisiae and protease is mixed;
(7) solid fermentation: culture medium is moved into fermentation vat temperature controlled fermentation 32h ~ 48h, fermentation temperature controls at 35 ~ 38 DEG C;
(8) two-step fermentation culture medium: the culture medium after terminating to fermentation by saccharomyces cerevisiae adds 1 ~ 2% molasses, 0.1 ~ 0.5% acetic acid;
(9) actication of culture: mix by 1:30 ~ 50 weight ratio with 30 ~ 35 DEG C of warm water with bacillus subtilis, Bacillus acidi lactici, stirs until bacterial classification is dissolved in the water completely, activation 20 ~ 30min;
(10) inoculate: by inoculative proportion inoculation bacillus subtilis and the Bacillus acidi lactici of bacillus subtilis 2 ~ 3%, Bacillus acidi lactici 3 ~ 5%, mix;
(11) two-step fermentation: culture medium is moved into fermentation vat, temperature controlled fermentation 48 ~ 72h, fermentation temperature controls at 28 ~ 32 DEG C;
(12) low temperature drying: after fermentation ends, low temperature drying material is to moisture 10 ~ 12%;
(13) pulverize: the peptide that will ferment after fermentation ends is pulverized, cross 60 mesh sieves;
(14) composite: by sodium selenite and medical stone respectively in biofermentation peptide mass ratio 2 ~ 3%, 3 ~ 5% ratio add;
(15) mix: biofermentation peptide, sodium selenite, medical stone are mixed and obtain fodder for increasing milk biofermentation peptide feed addictive.
2. fodder for increasing milk biofermentation peptide according to claim 1, is characterized in that the described assorted dregs of rice are one or both in sesame seed meal, peanut meal, Cottonseed Meal, rapeseed dregs.
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