CN102813057B - Method for producing fermented feed through utilizing enteromorpha leaching liquor - Google Patents
Method for producing fermented feed through utilizing enteromorpha leaching liquor Download PDFInfo
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- CN102813057B CN102813057B CN201210347404.9A CN201210347404A CN102813057B CN 102813057 B CN102813057 B CN 102813057B CN 201210347404 A CN201210347404 A CN 201210347404A CN 102813057 B CN102813057 B CN 102813057B
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- enteromorpha
- leaching liquor
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Abstract
The invention belongs to the field of microbial fermented feeds, and in particular relates to a method for producing a fermented feed through utilizing enteromorpha leaching liquor, wherein dry enteromorpha which is coarsely ground is soaked in the water, then uniformly mixed and separated; then, the mixture is mixed: feed, fermented strain and the enteromorpha leaching liquor are put in a blender mixer to be mixed, and then, the mixed material is uniformly discharged; and finally, the mixed material is fermented: the mixed material is uniformly laid in a room, subjected to thermal insulation treatment, fermented for 37-72 hours and dried, and thus, the microbial fermented feed is produced. The method has the advantages that the activity of the compound strain in the fermented feed can be stimulated, and the nutritional ingredients of the feed, which cannot be easily absorbed by livestock directly, are degraded into micromolecular substances which can be absorbed and digested, such as various amino acids, small peptides and the like; and moreover, the number of putrefying bacteria in the feed is reduced, and the probability of causing digestive canal diseases after the livestock eat the feed is decreased.
Description
Technical field
The invention belongs to fermentative feedstuff of microbe field, be specifically related to a kind of method of utilizing Enteromorpha leaching liquor to produce fermented feed.
Background technology
Along with the fast development of livestock breeding industry, the contradiction that people holds common grain is increasingly outstanding, and research has become with the new way of exploitation feed resource the emphasis that people study, and wherein fermentative feedstuff of microbe more and more comes into one's own.
Fermentative feedstuff of microbe refers under artificial controllable condition, taking vegetalitas agricultural byproducts as primary raw material, by the metabolism of microorganism, the macromolecular substances such as degraded part polysaccharide, protein and fat, generate the small-molecule substances such as organic acid, soluble polypeptide, form nutritious, good palatability, biological feedstuff or feedstuff that useful viable bacteria content is high.Microorganism is just decomposed the nutriment such as glucose, protein of feed during the fermentation in advance, contribute to domestic animal feed absorption, digestion afterwards, in fermented feed, there is a large amount of probios to exist simultaneously, can suppress the harmful bacteria in feed, the pathogenetic probability of gastral disease that reduces domestic animal, this is unexistent in conventional feed.And fermented feed has fermenting aroma, good palatability, livestock is liked to eat.
Enteromorpha is a genus of Chlorophyta Ulvaceae, and Enteromorpha self is rich in carbohydrate, protein, crude fibre and mineral matter, also contains fat and vitamin simultaneously.And Enteromorpha can reduce the pH value of Moisture in Feed, regulate the metabolic process of microorganism, if Enteromorpha can be used for to the production of fermentative feedstuff of microbe, will promote so the activity of bacterial classification in fermented feed, improve the Fermentation efficiency of feed, reduce antibiotic use in domestic animal nursing, the security that has improved food.
Summary of the invention
The object of this invention is to provide a kind of method of utilizing Enteromorpha leaching liquor to produce fermented feed, biochemical reaction by Enteromorpha leaching liquor can effectively improve the nutritional labeling in fermented feed, shorten the feed fermentation time, reduce the putrefactivebacteria amount in fermented feed, thereby guaranteed the quality of fermented feed.
In order to realize above technique effect, the present invention realizes as follows:
(1) preparation of Enteromorpha leaching liquor: the dry Enteromorpha after coarse crushing is immersed in the water, then carries out separating after uniform stirring;
(2) mixing of mixed material: feed, fermented bacterium and Enteromorpha leaching liquor are put into batch mixer and stir, mixed material is discharged after evenly;
(3) fermentation of mixed material: mixed material uniform spreading is located at indoor, and is incubated processing, ferment after 37-72 hour, mixed material is dry.
In described step (1), will be dried Enteromorpha coarse crushing to 40 order-50 order, the moisture content 6%-10% of described dry Enteromorpha.
In described step (1), dry Enteromorpha is mixed by the weight proportion of 1:900-1100 with water, be heated to 45 DEG C-50 DEG C and slowly at the uniform velocity stirring, after 1-1.5 hour, Separation of Solid and Liquid is got Enteromorpha leaching liquor.
Fermented bacterium in described step (2) is one or more of bifidocaterium, lactic acid bacteria, bacillus, photosynthetic bacteria, saccharomycete and aspergillus niger, and wherein zymophyte content is 1 × 10
9cfu/ml-1 × 10
10cfu/ml.
In described step (2), feed, fermented bacterium and Enteromorpha leaching liquor according to 1kg:0.4 × 10
9cfu-2 × 10
10cfu:0.5kg-1kg mixes, and incorporation time is 5-10 minute, and described feed is the dish dregs of rice, dregs of beans or cotton dregs.
In described step (2), add 0.08 ‰-0.34 ‰ assisted fermentation agent of mixed material total amount during to 2-4 minute at batch mixer mixed material.
Described assisted fermentation agent is sucrose.
In described step (3), be 5-10 rice by mixed material according to length, width is 1.5-2 rice, is highly located on indoor plank or concrete floor for 0.2-0.3 rice uniform spreading.
The method of the insulation processing in described step (3) is, on mixed material, builds roof insulating with film, and this roof insulating is 1-1.5 rice apart from the height of mixed material.
In described step (3), at 10 DEG C-35 DEG C, insulation is processed.
The invention has the beneficial effects as follows: the present invention is a kind of technique of the technical production fermented feed through improvement, add in process of production Enteromorpha leaching liquor, just be rich in the nutriments such as protein, carbohydrate, vitamin due to Enteromorpha itself, and Enteromorpha also can promote the activity of the composite bacteria in fermented feed, can better the nutritional labeling that is not easy directly to be absorbed by domestic animal in feed be resolved into the small-molecule substance that can be absorbed, digest, as each amino acid, little peptide etc., can effectively ensure like this fine quality of fermented feed; Can also reduce the putrefactivebacteria number in feed, reduce the incidence rate of domestic animal at the edible rear disease of digestive tract causing, thereby reduce antibiotic use amount, improved the security of the carnivorous food of domestic animal simultaneously.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
Feed is dregs of beans:
Be 10% by the water content that is crushed to dry this dry Enteromorpha of Enteromorpha 0.5kg(of 40 objects) insert in heated type mixer and add 500kg water, mixer carries out low rate mixing, this splash bar rotating speed is 5rpm/ minute, in stirring, heat, while being heated to 45 DEG C, constant temperature stirs, and adds thermal agitation and continues 1 hour.Mixed liquor is passed into Rose Box filter cloth and separate, the leaching liquor after separation is faint yellow, pours into container for subsequent use.By 1000kg dregs of beans, 0.5L composite zymocyte, wherein zymophyte content is 9 × 10
9cfu/ml-12 × 10
9cfu/ml.(the special EM stoste of cultivation that Anhui Guang Yu Bioisystech Co., Ltd produces, its main component is bifidocaterium, lactic acid bacteria, bacillus, photosynthetic bacteria, saccharomycete) and 500kg Enteromorpha leaching liquor put into batch mixer and stir, agitator shaft speed is 20rpm/ minute, while being mixed to 2 minutes, pour 0.5kg sucrose into and continue to mix, mixing of materials is discharged after being mixed to 8 minutes.
Mixed material is layed on indoor cement flooring according to the specification of 7 meters of length, 1.5 meters of width, 0.3 meter of height, then builds roof insulating with transparent plastic film, changing roof insulating is 1 meter apart from the height of mixed material.Fermentation time is 48 hours, and the dregs of beans entering after everfermentation has micro-acid gas taste, and moisture content is 32%.After having fermented, remove roof insulating, after fermented bean dregs is collected, carry out stored dry.
Nutritional labeling contrast (table one) in Enteromorpha leaching liquor fermented bean dregs and traditional zymotic dregs of beans:
| Dregs of beans | Traditional zymotic dregs of beans | Leaching liquor fermented bean dregs | |
| Crude protein % | 41.3 | 50.5 | 51.6 |
| Crude fibre % | 5.8 | 3.3 | 3.1 |
| Little peptide content % | 0.5 | 8.1 | 9.8 |
| VBN mg/100g | 6.8 | 7.7 | 7.4 |
Known from table one, utilize the dregs of beans of Enteromorpha leaching liquor fermentation to rise 2% than the crude protein content in traditional zymotic dregs of beans, little peptide content has risen 21%, and VBN (mg/100g) content has declined 5.2%, and crude fibre has reduced by 6.1%.
Embodiment 2
Feed is cotton dregs
Be 10% by the moisture content that is crushed to dry this dry Enteromorpha of Enteromorpha 1kg(of 40 objects) insert heated type mixer and add 1000kg water.Mixer carries out low rate mixing, and splash bar rotating speed is 5rpm/ minute, heats simultaneously, and while being heated to 50 DEG C, constant temperature stirs, and adds thermal agitation and continues 1 hour.Then, mixed liquor is passed into Rose Box filter cloth Enteromorpha is separated with leaching liquor, the leaching liquor after separation is faint yellow, pours into container for subsequent use.By 2000kg cotton dregs, 2L aspergillus niger and lactic acid bacteria composite bacteria (wherein zymophyte content 5 × 10
9cfu/ml-2 × 10
10cfu/ml) and 1000kg Enteromorpha leaching liquor stir according to passing into together mixer, agitator shaft speed is 15rpm/ minute, pours 0.8kg sucrose into and proceed to mix while being mixed to 2 minutes, mixed material is discharged after being mixed to 10 minutes.Mixed material is layed on indoor cement flooring according to the specification of 10 meters of length, 2 meters of width, 0.3 meter of height, then builds roof insulating with transparent plastic film, changing roof insulating is 1 meter apart from the height of mixed material.Fermentation time is 72 hours, and the cotton dregs that enter after everfermentation have micro-acid gas taste, and moisture content is 35%.After having fermented, remove roof insulating, after fermentation cotton dregs are collected, carry out stored dry.
Nutritional labeling contrast (table two) in Enteromorpha leaching liquor fermentation cotton dregs and traditional zymotic cotton dregs:
| Cotton dregs | Traditional zymotic cotton dregs | Leaching liquor fermentation cotton dregs | |
| Crude protein % | 39.2 | 42.1 | 44.6 |
| Crude fibre % | 12.3 | 9.5 | 9.1 |
| Lysine % | 1.56 | 1.72 | 1.77 |
Known from table two, in the cotton dregs that utilize Enteromorpha leaching liquor to ferment, rise 6% than the crude protein content in traditional zymotic cotton dregs, lysine content has risen 3%, and crude fiber content has reduced by 4.2%.
Embodiment 3
Feed is the dish dregs of rice
Be 10% by the moisture content that is crushed to dry this dry Enteromorpha of Enteromorpha 0.3kg(of 40 objects) insert heated type mixer and add 300kg water.Mixer carries out low rate mixing, and splash bar rotating speed is 8rpm/ minute, heats simultaneously, and while being heated to 50 DEG C, constant temperature stirs, and adds thermal agitation and continues 1 hour.Then, mixed liquor is passed into Rose Box filter cloth Enteromorpha is separated with leaching liquor, the leaching liquor after separation is faint yellow, pours into container for subsequent use.By the 600kg dish dregs of rice, 0.3L composite bacteria liquid, wherein zymophyte content is 9 × 10
9cfu/ml-2 × 10
10cfu/ml, (its composition is saccharomycete, bacillus and lactic acid bacteria) and 600kg Enteromorpha leaching liquor stir according to passing into together mixer, agitator shaft speed is 20rpm/ minute, while being mixed to 2 minutes, pour 0.1kg sucrose into and continue to mix, mixed material is discharged after being mixed to 10 minutes.Mixed material is layed on indoor cement flooring according to the specification of 5 meters of length, 1.5 meters of width, 0.2 meter of height, then builds roof insulating with transparent plastic film, this roof insulating is 1 meter apart from the height of mixed material.Fermentation time is 37 hours, and the dish dregs of rice that enter after everfermentation have micro-acid gas taste, and moisture content is 38%.After having fermented, remove roof insulating, after the fermentation dish dregs of rice are collected, carry out stored dry.
Nutritional labeling contrast (table three) in the Enteromorpha leaching liquor fermentation dish dregs of rice and the traditional zymotic dish dregs of rice:
| The dish dregs of rice | The traditional zymotic dish dregs of rice | The leaching liquor fermentation dish dregs of rice | |
| Crude protein % | 35.8 | 40.1 | 41.3 |
| Crude fibre % | 11.1 | 8.4 | 8.2 |
| Lysine % | 1.4 | 1.66 | 1.67 |
Known from table three, utilize the dish dregs of rice of Enteromorpha leaching liquor fermentation to rise 3% than the crude protein content in the traditional zymotic dish dregs of rice, lysine content has risen 1%, and crude fiber content has reduced by 2.3%.
So, from the nutritional labeling contrast of above-mentioned three tables, can learn, utilize crude protein, the little peptide content of lysine etc. of the feed ratio traditional zymotic feed of Enteromorpha leaching liquor fermentation all to increase, do not declined by the crude fibre that domestic animal absorbed, the more horn of plenty of nutritional labeling of the fermented feed that this explanation employing the present invention produces.
Claims (6)
1. utilize Enteromorpha leaching liquor to produce a method for fermented feed, its step comprises:
(1) preparation of Enteromorpha leaching liquor: the dry Enteromorpha after coarse crushing is immersed in the water, described dry Enteromorpha mixes by the weight proportion of 1:900-1100 with water, and also slowly at the uniform velocity stirring Separation of Solid and Liquid after 1-1.5 hour gets Enteromorpha leaching liquor to be then heated to 45 DEG C-50 DEG C;
(2) mixing of mixed material: by feed, fermented bacterium and Enteromorpha leaching liquor according to 1kg:0.4 × 10
9cfu-2 × 10
10cfu:0.5kg-1kg puts into batch mixer and mixes, and incorporation time is after 5-10 minute, mixed material to be discharged;
Described feed is the dish dregs of rice, dregs of beans or cotton dregs;
Described fermented bacterium is one or more in bifidocaterium, lactic acid bacteria, bacillus, photosynthetic bacteria, saccharomycete and aspergillus niger, and wherein zymophyte content is 1 × 10
9cfu/ml-1 × 10
10cfu/mL;
(3) fermentation of mixed material: be 5-10 rice by mixed material according to length, width is 1.5-2 rice, is highly located on indoor plank or concrete floor for 0.2-0.3 rice uniform spreading, and is incubated processing, ferments after 37-72 hour, mixed material is dry.
2. a kind of method of utilizing Enteromorpha leaching liquor to produce fermented feed according to claim 1, is characterized in that: in described step (1), will be dried Enteromorpha coarse crushing to 40 order-50 order, the moisture content 6%-10% of described dry Enteromorpha.
3. a kind of Enteromorpha leaching liquor that utilizes according to claim 1 is produced the method for fermented feed, it is characterized in that: in described step (2), add 0.08 ‰-0.34 ‰ assisted fermentation agent of mixed material total amount at batch mixer mixed material during to 2-4 minute.
4. a kind of method of utilizing Enteromorpha leaching liquor to produce fermented feed according to claim 3, is characterized in that: described assisted fermentation agent is sucrose.
5. a kind of method of utilizing Enteromorpha leaching liquor to produce fermented feed according to claim 1, it is characterized in that: the method for the insulation processing in described step (3) is, on mixed material, build roof insulating with film, this roof insulating is 1-1.5 rice apart from the height of mixed material.
6. a kind of method of utilizing Enteromorpha leaching liquor to produce fermented feed according to claim 1, is characterized in that: described step (3) is incubated processing at 10 DEG C-35 DEG C.
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| CN201210347404.9A CN102813057B (en) | 2012-09-17 | 2012-09-17 | Method for producing fermented feed through utilizing enteromorpha leaching liquor |
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|---|---|---|---|
| CN201210347404.9A CN102813057B (en) | 2012-09-17 | 2012-09-17 | Method for producing fermented feed through utilizing enteromorpha leaching liquor |
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| CN102813057B true CN102813057B (en) | 2014-11-26 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103352016B (en) * | 2013-06-27 | 2015-03-11 | 中国海洋大学 | Method for preparing biological fertilizer by utilizing Alteromonas colwelliana A321 to ferment enteromorpha |
| CN103931886B (en) * | 2014-04-11 | 2016-08-24 | 集美大学 | A kind of enteromorpha feed additive, preparation method and its usage |
| CN106343175A (en) * | 2016-08-29 | 2017-01-25 | 界首市任寨乡天佑家庭农场 | Fodder used for producing functional beef high in DHA content |
| CN106490307A (en) * | 2016-12-14 | 2017-03-15 | 河南牧翔动物药业有限公司 | A kind of method of antimicrobial composition fermentation Entermorpha |
| CN115067424A (en) * | 2022-07-06 | 2022-09-20 | 福州天凯生物科技有限责任公司 | Enteromorpha fermentation preparation process |
Citations (5)
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|---|---|---|---|---|
| CN1168773A (en) * | 1997-04-30 | 1997-12-31 | 窦观一 | Fermanted algae paste and the use thereof |
| CN1181365A (en) * | 1997-11-21 | 1998-05-13 | 董巧云 | Method for preparing animal feed or fertilizer |
| AU2007200687A1 (en) * | 2006-02-23 | 2007-09-06 | Van Schaik's Bio Gro Pty Ltd | A seagrass based nutrient product for plant biological growth stimulation and a method for preparing the same |
| CN101669580A (en) * | 2009-09-21 | 2010-03-17 | 宁波大学 | Method for preparing feed additive by fermenting enteromorpha |
| CN102613400A (en) * | 2012-02-21 | 2012-08-01 | 山东大学威海分校 | Optimum processing technology of enteromorpha additive aquatic feeds |
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2012
- 2012-09-17 CN CN201210347404.9A patent/CN102813057B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168773A (en) * | 1997-04-30 | 1997-12-31 | 窦观一 | Fermanted algae paste and the use thereof |
| CN1181365A (en) * | 1997-11-21 | 1998-05-13 | 董巧云 | Method for preparing animal feed or fertilizer |
| AU2007200687A1 (en) * | 2006-02-23 | 2007-09-06 | Van Schaik's Bio Gro Pty Ltd | A seagrass based nutrient product for plant biological growth stimulation and a method for preparing the same |
| CN101669580A (en) * | 2009-09-21 | 2010-03-17 | 宁波大学 | Method for preparing feed additive by fermenting enteromorpha |
| CN102613400A (en) * | 2012-02-21 | 2012-08-01 | 山东大学威海分校 | Optimum processing technology of enteromorpha additive aquatic feeds |
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