CN104982648A - Wet state yeast culture movable fermentation method with distillers grains as raw materials - Google Patents
Wet state yeast culture movable fermentation method with distillers grains as raw materials Download PDFInfo
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- CN104982648A CN104982648A CN201510319359.XA CN201510319359A CN104982648A CN 104982648 A CN104982648 A CN 104982648A CN 201510319359 A CN201510319359 A CN 201510319359A CN 104982648 A CN104982648 A CN 104982648A
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Abstract
A wet state yeast culture movable fermentation method with distillers grains as raw materials comprises the following steps of batching, activation of culture, inoculation mixing, temperature-controlled fermentation, enzymolysis wall breaking and product postprocessing. The product postprocessing comprises inspection, packaging and finished products. The method adopts the bacterium and enzyme synergetic fermentation and enzymolysis technology with microzyme, protease and cellulose combined, the technology is good in stability and free of mold pollution, and products are safe. A two-step method temperature control technology is adopted, the number of microzyme is large after cultivating, enzymolysis is thorough, yeast wall breaking is sufficient, and the nutritive value is higher. The content of coarse fibers is more than 30% lower than conventional distillers grains and like products, the current situation that the conventional distillers grain products is only suitable for cud chewers is avoided, the using aspects of products are broadened, and market expansion is facilitated.
Description
Technical field
the present invention relates to a kind of is raw material with distillers ' grains, through the method for the feeding hygrometric state yeast culture of explained hereafter that fermentation and enzymolysis are worked in coordination with, belongs to technical field of bioengineering.
Background technology
Hygrometric state yeast culture is a kind of probiotics, mainly saccharomyces cerevisiae, adopts liquid, solid-stately combine or directly after solid medium top fermentation, process obtained product together with solid matrix under modern zymotechnique controls.This product is not containing a large amount of active dry yeasrs, and saccharomycete is a kind of instrument in yeast culture production, and whether the survival in finished product can not affect the result of use of product.Its Main Function composition is extracellular metabolic product, the solid matrix fermenting rear sex change, yeast cell contents and the yeast cell wall that culture propagation solid matrix produces.Product composition is complicated, containing mineral matter, B family vitamin, increase taste material, digestive ferment, manna oligosacchride, β ~ glucan and amino acid and " unknown somatomedin " etc., these materials are perfect nutritional substrate for the microorganism in animal gastrointestinal tract, can microbial growth be promoted, and then improve the production performance of animal.It is the microbial forage additive integrating the multi-efficiencies such as health care and nutrition.
Hygrometric state yeast culture can provide nutriment to microorganism in animal alimentary canal, thus excites micro-ecological environment in their metabolic activity and liptinite.Compared with antibiotics additive, there is green natural, have no side effect and good palatability, the advantage such as nutritious.
Function and the mechanism thereof of yeast culture are mainly:
(1) improve gastrointestinal bacterial flora to fall structure, improve breeding performonce fo animals,
(2) supplement the nutrients, improve digestive function,
(3) immunity of organisms and premunition is improved.
Diamond V Mill has just been had abroad since the nineties, the major companies such as Alltech develop a series of YC product all in succession, more domestic yeast culture producers also occur successively, obtain at U.S.'s yeast culture and generally used, at present from the yeast culture product that research is both at home and abroad more, mainly be divided into two large classes: the primary efficacy component of a series products is yeast cells extra-metabolite, as the Yikang series of yeast culture of Da Nongwei company, this product is one and contains minority saccharomycete, the mixture of the culture medium of sex change and the outer metabolin of yeast born of the same parents, saccharomycete is only as the instrument of production, whether survive in finished product and can not affect product effect, the primary efficacy component of another kind of product is active yeast cell, and as the yeast culture product that Alltech company produces, this product can tolerate intensification when ruminant tumor gastric sour environment and feed granulating.
China's white wine annual production is more than 1,000 ten thousand tons, and distillers ' grains is more than 2,000,000 tons, and because distillers ' grains moisture is large, content of cellulose is high, so all there is larger problem on transport is stored and be feeding.Current distillers ' grains only has small part to be used on ruminant, and major part does not all well utilize, and piles up corrupt, causes environmental pollution.Therefore by the process in engineering, remove rice husk in distillers ' grains, reduce water content, by fermentation enzymolysis processing, final drying forms product, develops high-quality feed raw material, has great significance.
Summary of the invention
for above-mentioned Problems existing, first object of the present invention is safe microorganisms bacterial strain and the enzyme preparation that research effectively can improve distillers ' grains nutritive value, works in coordination with technique provide basis for fermentation enzymolysis; Second object be to provide a kind ofly to can be mass-produced, the technique of bacterium enzyme associated treatment distillers ' grains that cost is low, simple to operate, produce the easy absorption being particularly suitable for various animal edible, the digestible nutritious and yeast culture feed of balance.
Technical solution of the present invention:
Be that the hygrometric state yeast culture of raw material moves a fermentation process with distillers ' grains, it is characterized in that, comprise the following steps:
1), prepare burden: add wheat bran in distillers ' grains, dregs of beans adjustment material forms mixture A and regulates mixture A water content 45 ~ 50%, crude protein content 25 ~ 28%.The albumen of wheat bran is slightly low, and about 16 ~ 19%, the albumen of dregs of beans is higher, about 41 ~ 43%, so general proportions scope is wheat bran: dregs of beans=5:2 ~ 5:3, this changes along with the protein content variation of raw material than regular meeting, therefore specifically calculates standard using water content and crude protein content as parameter.
2), actication of culture: Active Dry Yeast is added 20 ~ 25 times 35 ~ 37 DEG C containing sucrose 1 ~ 5% warm water in, stir evenly activation and obtain mixed solution B in 20 ~ 30 minutes.
3), inoculation mixing: the mixed solution B activated, protease, cellulase, molasses are mixed rear granulation with said mixture A.
4), temperature controlled fermentation: control temperature is 30 DEG C ~ 35 DEG C, ferments 24 ~ 36 hours, and period keeps air unobstructed.
5), enzymolysis broken wall: pile up and heat up, control temperature 45 ~ 50 DEG C of enzymolysis 24 ~ 48 hours.
6), product post processing, comprising: inspection → packaging → finished product.
Active dry yeast inoculum concentration of the present invention is 1 ~ 5 × 10
7individual/g siccative; Protease addition is 50 ~ 500u/g protein; Cellulase addition is 100 ~ 400u/g crude fibre.
Sweat of the present invention is that employing two Buwen degree controls, and reaches the abundant enzymolysis of protein and yeast broken wall, the object of releasing nutrients material.First step control temperature is 30 DEG C ~ 35 DEG C, ferments 24 ~ 36 hours, keeps ventilating; Second step is piled up and is heated up, control temperature 45 ~ 50 DEG C, enzymolysis 24 ~ 48 hours.
The present invention through the first step fermentation after, yeast viable count>=10 × 10
8cfu/g siccative; After second step enzymolysis, little peptide content>=10%, yeast sporoderm-broken rate>=80%.
The measuring method of above-mentioned bacterium number is: by sample dissolution in sterilized water, draws quantitative bacteria suspension and carries out concentration gradient dilution, is then applied on Martin's rose-bengal flat board by the bacterium liquid of variable concentrations respectively, Deng dull and stereotyped upper bacterium colony comparatively large after, count, calculate, its viable count can be obtained.
The present invention is compared with existing distillers ' grains zymotechnique, and tool has the following advantages and beneficial effect:
1, the fermentation enzymolysis process adopting the bacterium enzyme combined with saccharomycete and protease, cellulase to work in coordination with, technology stability is good, without mould contamination, product safety.
2, adopt two step method temperature control process, after cultivating, yeast count is high, thorough enzymolysis, and yeast broken wall is abundant, and nutritive value is higher.
3, this product coarse fiber content than conventional distillers ' grains and like product low by more than 30%, solve the present situation that conventional distillers ' grains product can only be suitable for ruminant, widened product use face, be conducive to the developing in market.
4, present invention process is simple, and cost is low, simple to operate, and parameter is easy to control, fermentation efficiency and quality high.
Detailed description of the invention
To look familiar in detail method of manufacture and use thereof of the present invention below in conjunction with embodiment.Embodiment provides many applicable inventive concepts, may be implemented in certain content miscellaneous.This description illustrated embodiment is only explanation the present invention, and is not used to the scope limiting the present invention.
The present invention includes the following step:
Be below to provided by the invention a kind of be that the hygrometric state yeast culture of raw material moves being described in further detail of fermentation process with distillers ' grains:
1), batching: add wheat bran in distillers ' grains, dregs of beans adjustment material forms mixture A and regulates mixture A water content control to be 45 ~ 50%, crude protein content is adjusted to 25 ~ 28%.
2), actication of culture: take Active Dry Yeast, make fermentation inoculum concentration be 1 ~ 5 × 10
7individual/gram siccative.By Active Dry Yeast, add 20 ~ 25 times 35 ~ 37 DEG C containing in the warm water of sucrose 1 ~ 5%, it is for subsequent use to stir evenly activation 20 ~ 30 minutes.
3), inoculation mixing: by the active dry yeast activated, protease (addition is 50 ~ 500 units/gram protein), cellulase (addition is 100 ~ 400 units/gram crude fibres), molasses (addition is 1 ~ 3%), mixing going that rear access prepares, in rice husk vinasse raw material, utilizes mixer fully to mix.
4), fermentation and enzymolysis: inoculate and mix rear granulation, load breathing zone, control temperature 30 DEG C ~ 35 DEG C, ferments 24 ~ 36 hours; Continue to pile up and heat up, control temperature 45 ~ 50 DEG C of enzymolysis 24 ~ 48 hours.
5), product post processing: refer to product fermentation enzymolysis complete after treatment process, comprise inspection → packaging → finished product.Testing index and packaging meet the regulation of relevant criterion.
Embodiment 1:
Distillers ' grains 1600kg, wheat bran 100kg, dregs of beans 100kg, (specification is living bacteria count 2 × 10 to Active Dry Yeast
10cfu/g) 1kg, add 10kg containing activation in the warm water of sucrose 1% 30 minutes, protease (specification is 50000 μ/g) 0.2kg, cellulase (specification is 50000 μ/g) 0.2kg, molasses 20k, mix, load breathing bag, control temperature 32 DEG C fermentation 36 hours, continue to pile up and heat up, control temperature is 50 DEG C of enzymolysis 24 hours, and packaging is product.
Embodiment 2:
Distillers ' grains 1500kg, wheat bran 100kg, dregs of beans 150kg, (specification is living bacteria count 2 × 10 to Active Dry Yeast
10cfu/g) 1.2kg, add 12kg containing activation in the warm water of sucrose 1.5% 30 minutes, protease (specification is 50000 μ/g) 0.3kg, cellulase (specification is 50000 μ/g) 0.2kg, molasses 30k, mix, load breathing bag, control temperature 30 DEG C fermentation 24 hours, continue to pile up and heat up, control temperature is 45 DEG C of enzymolysis 36 hours, and packaging is product.
Embodiment 3:
Distillers ' grains 1800kg, wheat bran 50kg, dregs of beans 50kg, (specification is living bacteria count 2 × 10 to Active Dry Yeast
10cfu/g) 2kg, add 20kg containing activation in the warm water of sucrose 2% 30 minutes, protease (specification is 50000 μ/g) 0.1kg, cellulase (specification is 50000 μ/g) 0.1kg, molasses 40k, mix, load breathing bag, control temperature 35 DEG C fermentation 36 hours, continue to pile up and heat up, control temperature is 50 DEG C of enzymolysis 24 hours, and packaging is product.
Embodiment 4:
Distillers ' grains 1200kg, wheat bran 100kg, dregs of beans 300kg, (specification is living bacteria count 2 × 10 to Active Dry Yeast
10cfu/g) 2kg, add 20kg containing activation in the warm water of sucrose 2% 30 minutes, protease (specification is 50000 μ/g) 0.4kg, cellulase (specification is 50000 μ/g) 0.1kg, molasses 40k, mix, load breathing bag, control temperature 30 DEG C fermentation 36 hours, continue to pile up and heat up, control temperature is 50 DEG C of enzymolysis 24 hours, and packaging is product.
Embodiment 5:
Distillers ' grains 1500kg, wheat bran 200kg, dregs of beans 50kg, (specification is living bacteria count 2 × 10 to Active Dry Yeast
10cfu/g) 1kg, add 10kg containing activation in the warm water of sucrose 1% 30 minutes, protease (specification is 50000 μ/g) 0.1kg, cellulase (specification is 50000 μ/g) 0.3kg, molasses 20k, mix, load breathing bag, control temperature 30 DEG C fermentation 36 hours, continue to pile up and heat up, control temperature is 45 DEG C of enzymolysis 36 hours, and packaging is product.
Although the present invention discloses as above with embodiment; so itself and be not used to limit the present invention; in the technical field of the invention any have usually know the knowledgeable; without departing from the spirit and scope of the invention; when doing various variations and retouching, the protection domain of therefore the present invention when depending on after the attached claim person of defining be as the criterion.
To sum up, the present invention produces a desired effect.
Claims (4)
1. be that the hygrometric state yeast culture of raw material moves a fermentation process with distillers ' grains, it is characterized in that, comprise the following steps:
1), prepare burden: add wheat bran in distillers ' grains, dregs of beans adjustment material forms mixture A and regulates mixture A water content 45 ~ 50%, crude protein content 25 ~ 28%;
2), actication of culture: Active Dry Yeast is added 20 ~ 25 times 35 ~ 37 DEG C containing sucrose 1 ~ 5% warm water in, stir evenly activation and obtain mixed solution B in 20 ~ 30 minutes;
3), inoculation mixing: the mixed solution B activated, protease, cellulase, molasses are mixed rear granulation with said mixture A;
4), temperature controlled fermentation: control temperature is 30 DEG C ~ 35 DEG C, ferments 24 ~ 36 hours;
5), enzymolysis broken wall: pile up and heat up, control temperature 45 ~ 50 DEG C of enzymolysis 24 ~ 48 hours;
6), product post processing, comprising: inspection → packaging → finished product.
2. a kind of is as claimed in claim 1 that the hygrometric state yeast culture of raw material moves fermentation process with distillers ' grains, it is characterized in that: described active dry yeast inoculum concentration is 1 ~ 5 × 10
7individual/g siccative; Protease addition is 50 ~ 500u/g protein; Cellulase addition is 100 ~ 400u/g crude fibre.
3. a kind of is as claimed in claim 1 that the hygrometric state yeast culture of raw material moves fermentation process with distillers ' grains, it is characterized in that: after step 5) fermentation, yeast viable count>=10 × 10
8cfu/g siccative.
4. a kind of is as claimed in claim 1 that the hygrometric state yeast culture of raw material moves fermentation process with distillers ' grains, it is characterized in that: after step 6) enzymolysis, controls little peptide content >=10%, yeast sporoderm-broken rate >=80%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105341324A (en) * | 2015-11-20 | 2016-02-24 | 南宁学院 | Preservation method of residual forage |
| CN107384716A (en) * | 2017-07-26 | 2017-11-24 | 恩施州施南春酒业有限公司 | A kind of brewing method of Organic Selenium healthy wine |
| CN110607246A (en) * | 2019-10-11 | 2019-12-24 | 福建省农业科学院农业工程技术研究所 | Yeast high-density propagation vinasse polypeptide molasses culture agent and preparation method thereof |
-
2015
- 2015-06-12 CN CN201510319359.XA patent/CN104982648A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105341324A (en) * | 2015-11-20 | 2016-02-24 | 南宁学院 | Preservation method of residual forage |
| CN107384716A (en) * | 2017-07-26 | 2017-11-24 | 恩施州施南春酒业有限公司 | A kind of brewing method of Organic Selenium healthy wine |
| CN110607246A (en) * | 2019-10-11 | 2019-12-24 | 福建省农业科学院农业工程技术研究所 | Yeast high-density propagation vinasse polypeptide molasses culture agent and preparation method thereof |
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Application publication date: 20151021 |