CN102357212B - Compound product obtained through supercritical CO2 extraction, and preparation method thereof - Google Patents
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Abstract
The invention discloses a compound product obtained through supercritical CO2 extraction, and a preparation method thereof. The method comprises the following steps of: performing supercritical CO2 extraction on a mixture of coix seed and Ganoderma spore which are taken as raw materials to obtain compound oil; adding water into raffinate and soaking, performing ultrasound assisted enzymolysis, and filtering and separating; adding nutrient substances into filtrate to prepare a culture medium, inoculating edible and medicinal strains, culturing, collecting, filtering and ultra-filtering; and finally adding soy protein isolate, mixing and homogenizing, and freeze-drying under vacuum to obtain the compound product. The compound product is prepared by the modern steps of extraction, enzymolysis, fermentation, ultrafiltration and homogenizing, is high in the content of Chinese herbal active ingredients, and has comprehensive nutrition, and pure mouthfeel and is convenient to take; and resources are recycled, cost is saved, and the method is a high-efficiency and environment-friendly technology and has obvious economic benefits.
Description
Technical field
The present invention relates to compound product and field of beverage, particularly a kind of employing supercritical CO
2Compound product of extraction and preparation method thereof.
Background technology
Semen Coicis is a kind of functional food ingredient of Medicine Food Homology, but for a long time food beauty care, the QI invigorating of making light of one's life by commiting suicide, heat clearing away eliminating dampness by diuresis, consumer edema and appetite stimulator are called the top grade medicine that nourishes heart in the Compendium of Material Medica.Semen Coicis contains protein 13.7%, fat 5.4%, carbohydrate 64.7%[Lv Feng etc., " low Peart barley fermented glutinous rice fabrication technique ", University Of Agriculture and Forestry In Fujian's journal (natural science edition), the 2nd phase of the 35th volume, in March, 2006].Wherein Semen Coicis oil has antitumor, cancerous cell is had to stop grow up and injury effect.Ganoderma spore is the propagated cell of Ganoderma, contains triterpenoid compound, fungus polysaccharide, aminoacid, electrolytes and minerals etc., and it has assembled the elite of Ganoderma, and various effective ingredient and content are all higher.Sporoderm-broken Ganoderma Lucidum Spore powder contains crude protein 18.12%, and aminoacid 5.869%[hole dawn is bright etc., and " Sporoderm-broken Ganoderma Lucidum Spore powder Analysis of Nutritive Composition ", edible fungi, 2008(2)].But immunity, the antitumor of the spore oil enhancing body that is made by Ganoderma spore powder, protect the liver nourishing the stomach, cardiovascular disease, hypertension, diabetes and neurasthenia are had good health care.
According to the characteristics of above-mentioned raw materials effective ingredient, adopt supercritical CO
2Abstraction technique extracts existing correlational study report to it.Yet, a lot of researchs just adopt above-mentioned single raw material to process, its goods effective ingredient is single, and the raffinate overwhelming majority behind the extraction oil is reduced to sub-quality products or defective work, for the production of feedstuff even discarded, the composition such as rich in protein, carbohydrate in the raffinate can't be fully efficiently utilized, these useful components larger effect can't be brought into play again.
Because the compositions such as the esters in the middle of Semen Coicis, the Ganoderma spore powder, terpenoid have mutual coordination, assist a ruler in governing a country in compatibility effect, therefore, two kinds of raw materials of coupling have entered research field.But with supercritical CO
2The compound preparation of abstraction technique preparation yet there are no relevant report.
Summary of the invention
The object of the present invention is to provide a kind of employing supercritical CO
2Compound product of extraction and preparation method thereof, the modern extraction of this product utilization, enzymolysis, fermentation, ultrafiltration, homogenizing are made, Chinese herbal medicine effective ingredients content height, comprehensive nutrition, pure, the taking convenience of mouthfeel, realize simultaneously resource reutilization, saved cost, be a kind of production technology of high-efficiency environment friendly, have significant economic benefit.
For achieving the above object, the present invention adopts following technical scheme:
Take Semen Coicis and Ganoderma spore powder as raw material, adopt first supercritical CO
2Extraction Semen Coicis and Ganoderma spore powder mixture get compound recipe oil; Then raffinate is soaked, ultrasonic assistance enzymolysis, isolated by filtration; Add nutrient substance in the filtrate again and be mixed with culture medium, access edible and medical fungi strain is through cultivation, collection, filtration, ultrafiltration; Add at last soybean protein isolate, compound product is made in mixing homogenizing, vacuum lyophilization.The parts by weight of described Semen Coicis and Ganoderma spore powder are: Semen Coicis 12-19 part, Ganoderma spore powder 1-8 part.
Its preparation method may further comprise the steps:
(1) pre-treatment: qualified Semen Coicis is pulverized, and it is for subsequent use to cross the 120-200 mesh sieve; Qualified ganoderma lucidium spore powder wall breaking is for subsequent use, sporoderm-broken rate 〉=90%; Two kinds of raw materials are mixed by the proportioning weighing, add drinking water or purified water and stir and make soft material, cross 10 mesh sieves and granulate, dry by the fire to material moisture≤6% in 50-60 ℃, it is for subsequent use to cross 50 mesh sieves; Particle diameter returns again less than 50 purpose powder and granulates;
(2) supercritical extraction: the pellet of step (1) is adopted supercritical CO
2Extraction, condition is: extraction kettle pressure 24-28MPa, extraction temperature 40-50 ℃, I level separating still pressure 8-12MPa, II level separating still pressure 6MPa, CO
2(food stage, purity 〉=99.5%) flow 100-150L/h, extraction time 4-5h, collect the material after extracting, centrifugalize: rotating speed 2000-3000r/min, time 5-10min gets compound recipe oil; Raffinate is for subsequent use;
(3) enzymolysis: with the raffinate weighing of step (2), soak 12h with drinking water or purified water, solid-liquid ratio is 1:10-20, regulates pH to 4.0-6.0, temperature 40-60 ℃, then add first cellulase reaction 1-2h, and add again mmp reaction 0.5-1h; The enzyme activity of described cellulase is 1,400,000 U/g, and consumption is the 0.01-0.05% of raffinate weight; Described protease, enzyme activity 200,000 U/g, consumption are the 0.01-0.05% of raffinate weight; Above-mentioned enzymolysis process all is to carry out ultrasonic frequency 25-30kHz under ultrasonic subsidiary conditions; Behind the enzyme digestion reaction feed liquid is warming up to 100 ℃, then enzyme denaturing 1-5min filters feed liquid filtrate for later use;
(4) preparation is cultivated: with the filtrate weighing of step (3), the magnesium sulfate of the 0.1-0.2% of adding filtrate weight and the potassium dihydrogen phosphate of 0.2-0.4%, be mixed with culture medium, the 30min that under 121 ℃ of conditions, sterilizes, sterilization accesses the edible and medical fungi strain in the culture medium after being cooled to room temperature, inoculum concentration 10-15%, at 120-180r/min, behind the cultivation 5-7d, collect and filter under 25-28 ℃ the condition, sieve number 60-100 gets fungus ball and bacterium liquid;
(5) ultrafiltration: be that the polysulfones asymmetric membrane of 10000D carries out ultrafiltration with the bacterium liquid molecular cut off of step (4), condition is: operating pressure 0.12-0.20MPa, and feed temperature 20-30 ℃, time 60-90min, it is for subsequent use to get concentrated solution;
(6) homogenizing, drying: weighing soybean protein isolates amount is 1.5-2.0 times of compound recipe oil quality, it is mixed through the colloid mill homogenizing with compound recipe oil, fungus ball, concentrated solution, its rotating grinding and fixing mill clearance control≤0.3mm, rotary speed 1000-1200r/min, make mixing of materials even, then with homogeneous solution routinely vacuum lyophilization to moisture≤5% namely get described compound product.
The described edible and medical fungi strain of step (4) is one or more of Cordyceps, Agaricus blazei Murrill, Ganoderma, Hericium erinaceus (Bull. Ex Fr.) Pers., Antrodia camphorata, Coriolous Dersicolor (Fr.) Quel, Inonqqus obliquus, Lentinus Edodes, Tremella, Grifola frondosa, Poria.
The described compound product of step (6) adds pharmaceutic adjuvant again and makes powder, capsule, tablet, pill or granule.
The described compound recipe oil of step (2) can be made compound recipe Semen Coicis G. lucidum spores Softgel or further be refined into oral liquid, beverage, infusion solutions or injection; Described raffinate through soak enzymolysis, preparation cultivation, filtration, collection, homogenizing, drying, pulverize, sieve, packing technique makes compound recipe Semen Coicis Ganoderma spore egg albumen powder solid beverage.
The present invention makes mixture with above-mentioned two kinds of raw materials through the science compatibility, adopts supercritical CO
2Technology extracts to get compound recipe oil, (the cellulose enzyme hydrolysis products is glucose with the raffinate enzymolysis simultaneously, the protease hydrolyzed product is peptone), make the abundant strippings of material such as C source, N source, vitamin, suitably add nutrient substance and be made into culture medium, inoculated and cultured, culture of isolated gets fungus ball, bacterium liquid (all contains abundant fungus polysaccharide, studies show that fungus polysaccharide has very strong anti-tumor activity, cancerous cell there is very strong restraint, and plays an important role at aspects such as immunomodulating, blood pressure lowering, blood fat reducing, antithrombotic, promoting digestion and protecting livers).The present invention the most at last compound recipe oil, fungus ball, bacterium liquid merges, adding soybean protein isolate (is a kind of dissolubility, the good vegetable protein of emulsibility, be rich in simultaneously the various essential amino acids of needed by human body, experimental results show that soybean protein isolate energy cholesterol reducing content, reduce the sickness rate of cardiovascular disease, have the effects such as anticancer, defying age, enhancing immunity) mix homogenizing, make the nutritional labeling height through series of processes such as vacuum lyophilizations, the goods of taking convenience.
Remarkable advantage of the present invention is:
(1) CO
2Nonpoisonous and tasteless, the product of its extraction does not have the residual of chemical solvent, edible safety.
The technological temperatures such as (2) extraction, enzymolysis, concentrated, lyophilizing are low, all are not higher than 60 ℃, easily keep the active component in the goods not to be destroyed.
(3) adopt corresponding enzyme that raffinate is carried out enzymolysis, both resource reutilization had been saved cost, again the materials such as C source, N source, vitamin was fully transformed stripping, had improved the batching of traditional liquid fermentation medium.
(4) compound product contains the effective ingredient such as Semen Coicis oil, coixenolide, Ganoderma spore oil, fungus polysaccharide, triterpenoid compound, protein, and preparation purity is high, composition is complete.
Description of drawings
Fig. 1 is process chart of the present invention.
The specific embodiment
The below is non-limiting examples of the present invention.
Embodiment 1: compound product
Raw material: Semen Coicis 5100g, Ganoderma spore powder 900g.
Strain: Cordyceps Paecilomyces hepiali.
Preparation method:
(1) pre-treatment: Semen Coicis is pulverized, and 120 orders sieve; With ganoderma lucidium spore powder wall breaking, sporoderm-broken rate after testing 〉=95%; Weighing mixes, and adds an amount of drinking water stirring and makes soft material, crosses the granulation of 10 mesh sieves, and in 55 ℃ of oven dry, moisture is 5.27% after testing, and it is for subsequent use that material is crossed 50 mesh sieves.
(2) supercritical extraction: material 6.00kg, condition is: the 24L supercritical CO
2Extraction equipment, extraction kettle pressure 26MPa, temperature 45 C; Separation reactor I pressure 8MPa; Separating still II pressure 6MPa; CO
2(food stage, purity 99.5%) flow 120L/h, extraction total time 5h per hour collects 1 time.Oil-water separation is carried out in collection, and is at 3000r/min, centrifugal that compound recipe is oily under the 5min condition; Raffinate is for subsequent use.Supercritical extraction result, compound recipe oil testing result sees Table 1, table 2.
Table 1 supercritical extraction result
Table 2 compound recipe oil testing result
(3) enzymolysis: raffinate 5300g is added drinking water 79500ml, soak 12h, regulating PH is 4.8, and then temperature 50 C adds first 1.06g cellulase reaction 1.5h, enzyme activity 1,400,000 U/g; After add again 1.06g mmp reaction 0.5h, enzyme activity 200,000 U/g; Enzymolysis process all is to carry out ultrasonic frequency 28KHz under ultrasonic subsidiary conditions.Behind the enzyme digestion reaction feed liquid is warming up to 100 ℃, then enzyme denaturing 5min filters feed liquid filtrate for later use.
(4) preparation is cultivated: add by following mass fraction: magnesium sulfate 120g, potassium dihydrogen phosphate 200g, be mixed with culture medium, then 30min sterilizes under 121 ℃ of conditions, sterilization accesses the Cordyceps Paecilomyces hepiali strain in the culture fluid after being cooled to room temperature, inoculum concentration 10% at 150r/min, is collected behind the cultivation 7d under 28 ℃ the condition and is filtered, sieve number 80 orders get fungus ball and bacterium liquid.Cultivation results sees Table 3.
Table 3 cultivation results
(5) ultrafiltration: it is that the polysulfones asymmetric membrane of 10000D carries out ultrafiltration that the bacterium liquid of step (4) is adopted molecular cut off, and condition is: operating pressure 0.15Mpa, and 25 ℃ of feed temperatures, time 70min, it is for subsequent use to get concentrated solution.
(6) homogenizing, drying: weighing soybean protein isolate 770g, it is mixed through the colloid mill homogenizing with compound recipe oil, fungus ball, concentrated solution, its rotating grinding is 0.2mm with fixing mill clearance control, rotary speed 1000r/min, the lyophilization of mix homogeneously final vacuum gets compound product, be total to 2389g, testing result sees Table 4.
Table 4 compound product testing result
Embodiment 2: compound product
Raw material: Semen Coicis 5000g, Ganoderma spore powder 1000g.
Strain: Agaricus blazei Murrill.
(1) pre-treatment: Semen Coicis is pulverized, and 120 orders sieve; With ganoderma lucidium spore powder wall breaking, sporoderm-broken rate after testing 〉=95%; Weighing mixes, and adds an amount of drinking water stirring and makes soft material, crosses the granulation of 10 mesh sieves, and in 55 ℃ of oven dry, moisture is 4.68% after testing, and it is for subsequent use that material is crossed 50 mesh sieves.
(2) supercritical extraction: material 6.00kg, condition is: 24L CO
2Supercritical extraction unit, extraction kettle pressure 28MPa, temperature 45 C; Separation reactor I pressure 10MPa; Separating still II pressure 6MPa; CO
2(food stage, purity 99.5%) flow 140L/h, extraction total time 5h per hour collects 1 time.Oil-water separation is carried out in collection, and is at 3000r/min, centrifugal that compound recipe is oily under the 8min condition; Raffinate is for subsequent use.Supercritical extraction result, compound recipe oil testing result sees Table 5, table 6.
Table 5 supercritical extraction result
Table 6 compound recipe oil testing result
(3) enzymolysis: weighing raffinate 5280g, add drinking water 79000ml, soak 12h, regulating PH is 6.0, then temperature 50 C adds first 1.05g cellulase reaction 1h, enzyme activity 1,400,000 U/g; After add again 1.05g mmp reaction 0.5h, enzyme activity 200,000 U/g; Enzymolysis process all is to carry out ultrasonic frequency 25KHz under ultrasonic subsidiary conditions.Behind the enzyme digestion reaction feed liquid is warming up to 100 ℃, then enzyme denaturing 5min filters feed liquid filtrate for later use.
(4) preparation is cultivated: add by following mass fraction: magnesium sulfate 100g, potassium dihydrogen phosphate 200g, be mixed with culture medium, then 30min sterilizes under 121 ℃ of conditions, after sterilization is cooled to room temperature Agaricus blazei is accessed in the culture fluid, inoculum concentration 10% at 160r/min, is collected behind the cultivation 6d under 25 ℃ the condition and is filtered, sieve number 60 orders get fungus ball and bacterium liquid.Cultivation results sees Table 7.
Table 7 cultivation results
(5) ultrafiltration: it is that the polysulfones asymmetric membrane of 10000D carries out ultrafiltration that the bacterium liquid of step (4) is adopted molecular cut off, and condition is: operating pressure 0.16Mpa, and 25 ℃ of feed temperatures, time 60min, it is for subsequent use to get concentrated solution.
(6) homogenizing, drying: weighing soybean protein isolate 1080g, it is mixed through the colloid mill homogenizing with compound recipe oil, fungus ball, concentrated solution, its rotating grinding is 0.2mm with fixing mill clearance control, rotary speed 1100r/min, the lyophilization of mix homogeneously final vacuum gets compound product, be total to 2647g, testing result sees Table 8.
Table 8 compound product testing result
Embodiment 3: compound recipe Semen Coicis Ganoderma spore egg albumen powder capsule
The compound product of embodiment 2 gained is made hard capsule through conventional technique, product specification 0.3g/ grain.
Embodiment 4: compound recipe Semen Coicis G. lucidum spores Softgel
Raw material: Semen Coicis 4500g, Ganoderma spore powder 1500g.
Preparation method:
(1) pre-treatment: Semen Coicis is pulverized, and 120 orders sieve; With ganoderma lucidium spore powder wall breaking, sporoderm-broken rate after testing 〉=95%; Weighing mixes, and adds an amount of drinking water stirring and makes soft material, crosses the granulation of 10 mesh sieves, and in 55 ℃ of oven dry, moisture is 4.73% after testing, and it is for subsequent use that material is crossed 50 mesh sieves.
(2) supercritical extraction: material 6.00kg, condition is: 24L CO
2Supercritical extraction unit, extraction kettle pressure 26MPa, temperature 45 C; Separation reactor I pressure 10MPa; Separating still II pressure 6MPa; CO
2(food stage, purity 99.5%) flow 130L/h, extraction total time 5h per hour collects 1 time.Oil-water separation is carried out in collection, and is at 3000r/min, centrifugal that compound recipe is oily under the 8min condition.Supercritical extraction the results are shown in Table 9.
Table 9 supercritical extraction result
(4) preparation: make compound recipe Semen Coicis G. lucidum spores Softgel, product specification 0.5g/ grain through conventional soft capsule technique.Testing result sees Table 10.
Table 10 compound recipe Semen Coicis G. lucidum spores Softgel testing result
Embodiment 5: compound recipe Semen Coicis Ganoderma egg albumen powder solid beverage
Raw material: from embodiment 4 supercritical COs
2Weighing 4500g is as raw material in the raffinate after the extraction.
Strain: Ganoderma.
Preparation method:
(1) soak enzymolysis: the 4500g raffinate is added drinking water 67500ml, soak 12h, regulating PH is 5.2, and then temperature 50 C adds first 0.90g cellulase reaction 1h, enzyme activity 1,400,000 U/g; After add again 0.90g mmp reaction 0.5h, enzyme activity 200,000 U/g; Enzymolysis process all is to carry out ultrasonic frequency 30KHz under ultrasonic subsidiary conditions.Behind the enzyme digestion reaction feed liquid is warming up to 100 ℃, then enzyme denaturing 5min filters feed liquid filtrate for later use.
(2) preparation is cultivated: add by following mass fraction: magnesium sulfate 70g, potassium dihydrogen phosphate 140g, be mixed with culture medium, then 30min sterilizes under 121 ℃ of conditions, after sterilization is cooled to room temperature Ganderma lucidum strain is accessed in the culture fluid, the Ganoderma inoculum concentration is 15%, at 160r/min, collects behind the cultivation 6d under 25 ℃ the condition and filters, sieve number 60 orders get fungus ball and bacterium liquid.Cultivation results sees Table 11.
Table 11 cultivation results
(3) ultrafiltration: it is that the polysulfones asymmetric membrane of 10000D carries out ultrafiltration that the bacterium liquid of step (2) is adopted molecular cut off, and condition is: operating pressure 0.15Mpa, and 25 ℃ of feed temperatures, time 90min, it is for subsequent use to get concentrated solution.
(4) homogenizing, drying: weighing soybean protein isolate 700g, it is mixed through the colloid mill homogenizing with fungus ball, concentrated solution, its rotating grinding is 0.2mm with fixing mill clearance control, and rotary speed 1000r/min carries out conventional vacuum lyophilization with homogeneous solution behind the mix homogeneously.Get compound product 1673g.
(5) crushing screening, packing: compound product is pulverized, 100 orders that sieve, compound recipe Semen Coicis Ganoderma egg albumen powder solid beverage is made in packing, product specification 2.0g/ bag.Testing result sees Table 12.
Table 12 compound recipe Semen Coicis Ganoderma egg albumen powder solid beverage testing result
The above only is preferred embodiment of the present invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (6)
1. one kind is adopted supercritical CO
2The preparation method of compound product of extraction is characterized in that: take Semen Coicis and Ganoderma spore powder as raw material, adopt first supercritical CO
2Extraction Semen Coicis and Ganoderma spore powder mixture get compound recipe oil; Then raffinate is soaked, ultrasonic assistance enzymolysis, isolated by filtration; Add nutrient substance in the filtrate again and be mixed with culture medium, access edible and medical fungi strain is through cultivation, collection, filtration, ultrafiltration; Add at last soybean protein isolate, mixing homogenizing, vacuum lyophilization make described compound product;
Described preparation method may further comprise the steps:
(1) pre-treatment: qualified Semen Coicis is pulverized, and it is for subsequent use to cross the 120-200 mesh sieve; Qualified ganoderma lucidium spore powder wall breaking is for subsequent use, sporoderm-broken rate 〉=90%; Two kinds of raw materials are mixed by the proportioning weighing, add drinking water or purified water and stir and make soft material, cross 10 mesh sieves and granulate, dry by the fire to material moisture≤6% in 50-60 ℃, it is for subsequent use to cross 50 mesh sieves;
(2) supercritical extraction: the pellet of step (1) is adopted supercritical CO
2Extraction, condition is: extraction kettle pressure 24-28MPa, extraction temperature 40-50 ℃, I level separating still pressure 8-12MPa, II level separating still pressure 6MPa, CO
2Flow 100-150L/h, extraction time 4-5h; Collection material, centrifugalize: rotating speed 2000-3000r/min, time 5-10min gets compound recipe oil; Raffinate is for subsequent use;
(3) enzymolysis: with the raffinate weighing of step (2), soak 12h with drinking water or purified water, solid-liquid ratio is 1:10-20, regulates pH to 4.0-6.0, temperature 40-60 ℃, then add first cellulase reaction 1-2h, and add again mmp reaction 0.5-1h; The enzyme activity of described cellulase is 1,400,000 U/g, and consumption is the 0.01-0.05% of raffinate weight; Described protease, enzyme activity 200,000 U/g, consumption are the 0.01-0.05% of raffinate weight; Described enzymolysis process all is to carry out ultrasonic frequency 25-30kHz under ultrasonic subsidiary conditions; Behind the enzyme digestion reaction feed liquid is warming up to 100 ℃, then enzyme denaturing 1-5min filters feed liquid filtrate for later use;
(4) preparation is cultivated: with the filtrate weighing of step (3), the magnesium sulfate of the 0.1-0.2% of adding filtrate weight and the potassium dihydrogen phosphate of 0.2-0.4%, be mixed with culture medium, the 30min that under 121 ℃ of conditions, sterilizes, sterilization accesses the edible and medical fungi strain in the culture medium after being cooled to room temperature, inoculum concentration 10-15%, at 120-180r/min, behind the cultivation 5-7d, collect and filter under 25-28 ℃ the condition, sieve number 60-100 gets fungus ball and bacterium liquid;
(5) ultrafiltration: be that the polysulfones asymmetric membrane of 10000D carries out ultrafiltration with the bacterium liquid molecular cut off of step (4), condition is: operating pressure 0.12-0.20MPa, and feed temperature 20-30 ℃, time 60-90min, it is for subsequent use to get concentrated solution;
(6) homogenizing, drying: weighing soybean protein isolates amount is 1.5-2.0 times of compound recipe oil quality, it is mixed through the colloid mill homogenizing with compound recipe oil, fungus ball, concentrated solution, its rotating grinding and fixing mill clearance control≤0.3mm, rotary speed 1000-1200r/min, make mixing of materials even, then with homogeneous solution routinely vacuum lyophilization to moisture≤5% namely get described compound product.
2. employing supercritical CO according to claim 1
2The preparation method of the compound product of extraction, it is characterized in that: the parts by weight of raw material are: Semen Coicis 12-19 part, Ganoderma spore powder 1-8 part.
3. employing supercritical CO according to claim 1
2The preparation method of compound product of extraction is characterized in that: the described edible and medical fungi strain of step (4) is one or more of Cordyceps, Agaricus blazei Murrill, Ganoderma, Hericium erinaceus (Bull. Ex Fr.) Pers., Antrodia camphorata, Coriolous Dersicolor (Fr.) Quel, Inonqqus obliquus, Lentinus Edodes, Tremella, Grifola frondosa, Poria.
4. employing supercritical CO according to claim 1
2The preparation method of the compound product of extraction, it is characterized in that: the described compound product of step (6) adds pharmaceutic adjuvant again and makes powder, capsule, tablet, pill or granule.
5. employing supercritical CO according to claim 1
2The preparation method of the compound product of extraction, it is characterized in that: the described compound recipe oil of step (2) can be made compound recipe Semen Coicis G. lucidum spores Softgel or further be refined into oral liquid, beverage, infusion solutions or injection.
6. employing supercritical CO according to claim 1
2The preparation method of compound product of extraction is characterized in that: the described raffinate of step (2) through soak enzymolysis, preparation cultivation, filtration, collection, homogenizing, drying, pulverize, sieve, packing technique makes compound recipe Semen Coicis Ganoderma spore egg albumen powder solid beverage.
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| CN104738215B (en) * | 2015-04-01 | 2017-09-12 | 福建拓天生物科技有限公司 | A kind of camellia oil and its processing method for improving immunity |
| CN105661629B (en) * | 2016-01-15 | 2018-03-23 | 深圳市仁泰生物科技有限公司 | Antrodia cigarette and preparation method thereof |
| CN105876707A (en) * | 2016-04-26 | 2016-08-24 | 申世斌 | Hericium erinaceus peptone and processing method thereof |
| CN108938679A (en) * | 2018-07-30 | 2018-12-07 | 福州迈康医药科技有限公司 | A kind of transparent capsule and pill of Antrodia camphorata extract |
| CN117448078B (en) * | 2023-12-13 | 2024-05-14 | 广州市浩立生物科技有限公司 | Combined enzymolysis technology and supercritical CO2Extracted coffee oil and preparation method thereof |
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