WO1995018626A1

WO1995018626A1 - A tonic drug made from basidiomycetes, algae, chinese herbal medicine and the method thereof

Info

Publication number: WO1995018626A1
Application number: PCT/CN1994/000070
Authority: WO
Inventors: Lushan Lin
Original assignee: Lushan Lin
Priority date: 1994-01-08
Filing date: 1994-09-02
Publication date: 1995-07-13
Also published as: CN1104902A; CN1063331C; AU7530094A

Abstract

The invention discloses a tonic drug made from basidiomycetes, algae, chinese herbal medicine and hydrolases by method of biological fermentation and the method thereof. The description of the drug comprises 32-48 % by weight of basidiomycetes and algae and 52-68 % by weight of chinese herbal medicine. Said drug contains large amount of small molecular active proteins and polypeptides, enriched in nutritions and is effective in health protection, senile-resistance and treating some difficult and complicated cases of illness.

Description

Multi-encouraging health-care medicines based on seed, sea and Chinese leather medicines and their preparation methods

The invention relates to a biological fermentation product and a preparation method thereof, in particular to a health care medicine obtained by using a fermentation fermentation technology and a preparation method thereof. Background technique

In traditional Chinese medicine, there are some examples of the application of certain fungi such as basidiomycetes and fungi, seaweeds and Chinese herbal medicine to treat certain diseases. '然' And this is a curative effect. It is a Chinese medicinal material formulated for use in the clinic after being prepared and cut. Rhenium is that in the actual preparation process of Chinese medicinal materials, it is often difficult to grasp the heating temperature or increase the toxicity of the medicine, for example, the sand at the high temperature will precipitate free mercury, which will increase the toxicity of the drug; or due to improper heating Some medicines have their active ingredients destroyed, and they have a colorful effect. For example, proprietary Chinese medicines such as Helei pills, pollen, and royal jelly often show a decrease in the amount of original proteins and amino acids. In the process of processing volatile drugs, the reduction of volatile oil content is more obvious. In addition, improper control of the solubility of the drug in water during processing can affect the completeness. In short, the processing of raw materials in traditional Chinese medicine cannot accurately reflect the overall comprehensive effectiveness of raw materials.

^ Month

The purpose of the present invention is to overcome the shortcomings of the prior art, and to provide a biological fermentation product with multiple health-enhancing and therapeutic effects, which is mainly composed of fungi, seaweed and Chinese herbal medicine _β

Another object of the present invention is to provide a method for preparing the above-mentioned biological fermentation product, so as to avoid the disadvantageous color noise generated by using traditional Chinese medicine processing methods, such as high temperature heating.

The present invention relates to a biological fermentation product, which comprises the fruit bodies and seaweeds of Basidiomycetes 32-48 * based on the total weight of the main family, and 52-68 * Chinese herbal medicines based on the original protoplast, bacterial oxalase, and Fermentation products with enzyme-promoting agent, carbon source, and nutritional supplements as supplementary raw materials, of which:

The weight ratio of the basidiomycete to the seaweed is 2: 0. 5-1. 5;

The basidiomycete is selected from one or more kinds of lentinus, edulis, Poria, Hog, Poria, Ganoderma lucidum, Hericium erinaceus, mushroom, Tremella;

The seaweed is selected from one or two of kelp, laver, seaweed, and hairweed;

The bacterial oxalase is selected from one or more of α-amylase, cellulase, pectinase, and fungal protease, and the added rhenium is 0.01-0.0 of the total weight of the raw materials;

The enzyme-promoting agent is selected from yeast mash or corn slurry or a mixture thereof;

The nitrogen source and carbon source are selected from the group consisting of peptone, bran, fine bran, sweet potato flour, corn flour, bran, rice bran, One or more of mashed potato powder, tulipa powder, bean cake powder, peanut cake powder, rapeseed cake powder, black sesame powder, brown sugar, and white sugar;

The nutritional additives are inorganic salts and substances combined with phosphorus, calcium, potassium, magnesium, selenium, and zinc; the weight ratio of the main raw material to the fermentation promoter, nitrogen source, carbon source, and nutritional additive is 100: 0.02 -0.1: 0.18-0.22: 4.6-5.4: 0.14-0.16.

The method for preparing a biological fermentation product of the present invention includes the following steps:

(1) The main drug source of the drug is taken, which includes 32-48 weight percent of fungi and seaweed, and 52-68 weight percent of Chinese herbal medicine; bacterial oxalase; the enzyme production promoter, nitrogen source, carbon source and Nutritional additives as pharmaceutical auxiliary materials;

(2) pulverizing the above-mentioned fungi and seaweed and the above-mentioned Chinese herbal medicine into powders;

(3) immersing the lactic acid aqueous solution for the Chinese herbal medicine powder for 24 hours, and then adding the pulverized fungi and seaweed powder, adding the above-mentioned drug-assisted progenitors under mixing and stirring, and thoroughly stirring to obtain a mixed raw material and sterilizing;

(4) Put the mixed protoplast and bacterial oxalase obtained in (3) in a bioreactor, adjust the pH to 4.0-6.5 with lactic acid at 30-55 ^e C, and culture for 5-8 days with aeration, stirring daily 1-2 times, and starting at the 24th hour of fermentation, every 24 hours, a mixture of a basidiomycete puree and / or seaweed pulp that accounts for 1-2¾ of the total weight of the main raw material is infused, and the number of inoculations is 2-3 times To obtain a fermentation product;

(5) Centrifuging and discarding the fermentation product, decolorizing it by adsorption, concentrating, and drying it to make a product.

The choice of various kinds of raw materials and their amounts have the same definition as above. The various bacterial oxalase enzymes are prepared as follows:

The 0! -Amylase is derived from corn starch 2, corn slurry 26 *, disodium hydrogen phosphate 37 *, calcium fluoride

1%, potassium fluoride 0.2¾, hafnium fluoride 0.2%, bran 9%, red yeast rice culture product of pig pancreas, where the percentage is weight percentage;

The fungal protease is derived from corn starch 30¾, corn slurry 5 *, soy flour 5¾, casein gelatin dried distiller's grains 5¾, dipotassium phosphate 2.4%, sodium nitrate 1 ammonium gasification: ^, iron sulphate 0.01 bran 5 *, Fine bran 5 *, culture product of Ganoderma spore pink koji ½, wherein the percentage is weight percentage; the cellulase is derived from edible fungus green waste fungus 65¾ί, bran 10%, bran 10 lucic acid Solid fermentation product of ammonium 2.5%, water 250%, yeast expensive 1 Tween 80.0.05%, crude brown sugar 1 red yeast rice 1 *, the percentage of which is weight percentage;

Or derived from rice bran 1%, bran 0.5 ammonium sulphate 0.2 potassium dihydrogen phosphate 0.1, calcium carbonate 0.1%, glucomannose 1 * in a triangle flask, 30 ^e C, 104 rpm, culture 32-35 hours, The first-stage fermentation product of the seed was obtained; the waste fungus material 6 bran 0.5¾, glucosamine ammonium gluconate 0.2% potassium dihydrogen phosphate 0.1% were at 30 ^e C, 104 Turn / min and culture for 60 hours to obtain a secondary seed culture; seed primary and secondary cultures, peptone 0.075%, Tween 80 0.2%, ammonium sulphate 1.4g / I potassium dihydrogen phosphate S. Og / L urea O g / Is gasification calcium 0.3g / L, magnesium sulfur 0.3g / I ferric sulfur 5. (^ / manganese LG sulfur / sulfur LG zinc / cobalt gasifier ₉ 2.0 / 1 ^ and Wave body fermentation product using polymalonate having a molecular weight of 2000 as an antifoaming agent, wherein the percentage is a weight percentage;

The pectinase is derived from 4% of pectin, 0.4% of peptone, 0.05% of sodium sulphate, 0.01% of iron sulphate, 0.05% of magnesium sulphate, 0.05% of potassium vaporization, 55% of potassium dihydrogen phosphate bran, 5% fine bran, sucrose 2%, ammonium nitrate 0.2%, sodium nitrate 0.2 and connected to the spore suspension wave of ganoderma spores containing 2 10 ³ spores, 30-C, culture product obtained by culturing in a conical flask for 7 days, where the percentage is weight percentage .

The above preparation method of the present invention enables some ingredients in Chinese herbal medicines to be changed during the preparation process, that is, the molecular structure of the drug components is changed, so that it can most effectively exert the persuasion of the original subjects, so that the prepared medicine has more It is suggested that health care can be effective, and some difficult diseases of Meike are treated, and the preparation steps are simple and easy.

The present invention is described in detail below. '

The biological fermentation product of the present invention is preferably the following combined fermentation product of the original family, and each component used in the preparation method thereof has the following weight percentages based on the total weight of the raw materials:

Biological fermentation ω:

The basidiomycetes and seaweeds have the following weight percentages: Lentinus edodes 7-8¾, Flammulina velutipes 18-19¾, Poria 1-2%, Ganoderma 2-3 kelp 14-16¾;

The Chinese herbal medicine has the following weight percentage components: Boziren 7-8 *, Shezizi 3-4 Cuscuta 3-4 Raspberry 3-4%, Dendrobium 2-3%, Morinda officinalis 1-2, Continued off 4-5 Black medicine 0.5-1%, Calamus 1-1.5%, Alisma 0.5-1%, Chinese yam 1-2 ginseng 1-2%, 'Rehmannia glutinosa 2- 3 mulberry parasitic 0.5-1%, Dogwood 1-1 Schisandra 3-5%, stalactites 1-2¾, Cistanche 2-3% and ox vine 0.7-1¾ί;

The auxiliary raw materials are components with the following weight percentages: peptone 0.01-0.02%, brown sugar 0.2-0.3%, stone powder: 1, KCI 0.4-0.5%, MgSO ₄ 0. -0.5%, KH ₂ PO ₄ 0.4-0.5%,

(NH ₄ ) ₂ SO ₄ 0.3-0.35%;

The bacterial oxalase ^ fiber's enzyme produced by the above method;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: Enoki mushroom puree, mushroom puree, Ganoderma lucidum and kelp puree with a weight ratio of 2: 1.

Biological Ferment (2):

The basidiomycetes and seaweeds have the following weight percentages: Shiitake mushrooms 7-8%, Enoki mushroom 4-6 soil Poria 1-2¾, Ganoderma 2-3¾, kelp 7-8%;

The Chinese herbal medicine has the following weight percentage components: Bo Zi Ren 4-5 *, She Chuang Zi 4-4.5 菟 Silk seeds 4-4.5%, raspberries 4-4.5 <fe, Dendrobium 1-1.5¾ ;, Morinda officinalis 1.-1.5%, Eucommia ulmoides 1-1.5 Asparagus 1-1.5%, Zhiyuan (heart removal) 1 ^: 1.5 ½: Zhiren 0.2-0.5 continuity 0.5-0.7¾ ;, Wuya 0.5-0.7 alfalfa 0.7-1 Alisma 0.7-1S ;, yam 0.8-ί, ginseng 0.8-1 rehmannia 0.5-0.8 dogwood S.6- 1%, Five Gardenias 2-3 Stalactites (refined) 1-1.2¾ ;, Cistanche 2-3

The auxiliary progenitors are components having the following weight percentages: bran 7-8%, fine bran 7-8 sweet potato powder 4-4.2%, yeast 1-1.2¾f, sucrose, calcium carbonate 1-1.2

The bacterial oxalase is a cellulase made by the above method;

Biological Ferment (3):

The basidiomycetes and seaweeds have the following weight percentages: Poria cocos, Potentilla 7-8¾, kelp 7-8¾;

The Chinese herbal medicine has the following weight percentage components: Polygonum multiflorum (manufactured) 5-6%, Achyranthes bidentata 3-4¾, Cuscuta 3-4, Ligustrum 3-4%, Eucommia ulmoides 4-5, County sign grass 5- 6%, mulberry leaves 2-3%, silver flowers 3-4%, black mulberries 3-4%, medlar 0.3-0., Chrysanthemums 5 -6% Dihuang ³ _4¾, mangosteen 3-4¾, tannin ³ _ ⁴ %, Alisma 0.3-0.4¾, yam 3-4%;

The auxiliary raw tincture is a component having the following weight percentages: bran 7-, fine bran 7-8%, yeast 1-1.2%, sucrose 1-1.2%, calcium carbonate 1-1.2 *;

The bacterial oxalase is 0.02 * cellulase and 0.01% pectinase;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake mushroom puree, mushroom puree, kelp puree.

Biological Ferment "):

The basidiomycetes and seaweeds have the following weight percentages: Lentinus 3-, Mushroom 3-4%, Hericium 3- «, Kelp 5-6%;

The Chinese herbal medicine has the following weight percentage components: Huangyaozi 10-20 *, Chuandi 4-5 *, Shayuanzi 4-5, Oyster 3-4¾, Curcuma 3-4%, Coix Seed 3-, Chuanxiongzi 4 -5 green peel 3 -4%, 蜈蚣 1.2-1.5%, spot §1.2-1.5 *, talc 3-4¾ί, unicorn 3-4 amomum 2-3¾ !, chicken gizzard inside 2-3%, codonopsis 2 -3%, astragalus 3-6¾, cooked rehmannia 3-6 *, 'er ug 3-6½, betel nut slices 3-6%, Sichuan army 1-2 yuan Ming powder 1-2 dried toad 0.5-1 *, acute 0.6 -1, 2 Zhuru ² · 3 <¾ and ochre 4-5 *;

The auxiliary raw materials are components having the following weight percentages: bran 4-5 fine bran 4-5%, yeast 1.2-1.5%, brown sugar 3-, soybean powder 3-, calcium carbonate 1-1.2%;

The bacterial oxalase is 0.02¾ of the above-mentioned cellulase and 0.01% of the above-mentioned pectinase; The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake mushroom puree, kelp puree, and ganoderma puree.

Biological Ferment (5):

'The basidiomycetes and seaweeds have the following weight percentages: 10-15% Tremella, 1.5-4.5 Shiitake 5-;

The Chinese herbal medicine has the following weight percentage components: Xuanshen 3-, Ophiopogon 3-, Raw Rehmannia 3-4%, Chiba 1-2 Danshen 1-2S ;, Astragalus 3-4%, Yam 3- , Coix 1-2 *, Safflower 1_2 *, Bupleurum 0.5-5-0. 6 * and sea cucumber 7-8%;

The auxiliary raw materials are components having the following weight percentages: bran fine bran 6-8 sweet potato powder 6-8%, yeast 1.2-2¾, sucrose 1.2-2%, calcium carbonate 1-1.2

The bacterial oxalase is 0.02 * of the above-mentioned cellulase and 0.01% of the above-mentioned pectinase;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake mushroom puree, kelp puree, and ganoderma puree.

Biological Ferment (6):

The basidiomycetes and seaweeds have the following weight percentages: Lentinus 2-3, Poria cocos 1-2¾, Poria cocos 4-5%, Kelp 4-5;..

The Chinese herbal medicine has the following weight percentage components: Astragalus 1-2, Codonopsis 1-2 *, Fangfang 1-2, Atractylodes 1-2, Shouwu 2-4 *, Zexie 4-8, Hawthorn 2-4, Yinchen 2-4%, buffalo horns 2-4 *, epimedium 2-4 * and rhubarb

The auxiliary raw materials are components having the following weight percentages: bran 10-12%, fine bran 5-6¾, sweet potato flour 5-6%, baker's yeast 1-1.2 brown sugar 1-1.2 *, calcium carbonate 1-1.2 *;

The bacterial oxalase is 0.002¾; the above-mentioned cellulase O. 01 * ’s fungal protease; the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake puree, Mushroom puree, seaweed puree.

Biological Ferment (7):

The basidiomycetes and seaweeds have the following weight percentages: shiitake mushrooms 15-20%, kelp

The Chinese herbal medicine has the following weight percentage components: Polygonum multiflorum 18-22¾ ;, Ligustrum lucidum 9-10 *, Huai yam 9-10%, Mulberry and Hangbaiju ³ ·;

The auxiliary raw material is a component having the following weight percentages: bran 10-12 ^ fine bran 5-6¾, yeast 1-1.2 *, brown sugar 1.5-2 · 5 *, calcium carbonate 1-1.2 *;

The bacterial oxalase is the above-mentioned cellulase of 0.02 * and the above-mentioned amylase of 0.03Λ;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: Zhizhi puree, 佥 Needle mushroom puree, kelp puree.

The bacterial oxalase used in the present invention is a permanganase, but the pure hydrolyzed enzymes sold in the present invention are not effective because the enzymes are mainly produced by fungi and it is difficult to effectively treat Chinese herbal medicines. break down. The bacterial oxalase in the present invention is produced by co-fermentation of fungi and group fungi. When used, the above-mentioned cellulase is mainly used, or one of the above-mentioned cellulose is selected from the above-mentioned amylase, pectinase, and protease. Enzymes are used together.

Each step in the preparation method of the present invention is described in detail below.

1. Raw material selection:...

The basidiomycetes, algae, and Chinese herbal medicines of the present invention are all dry products with impurities removed, and the weight of each raw material is weighed according to the formula. ·, '·',

2. Crushing and grinding powder:

The above fungi and algae are pulverized and ground into powder, respectively, and the Chinese herbal medicine is pulverized into a 16-20 mesh fineness powder.

3, acidification treatment:

Soak the Chinese herbal medicine powder with 15% lactic acid for 24 hours, and then add the fungus and seaweed powder.

4. Mixing:

In the state of mixing and stirring each of the above raw materials, the enzyme promoter, ammonia source, carbon source, and nutritional additive of each auxiliary original product are further mixed, and then mixed and stirred evenly.

5. Inoculation fermentation:,

The present invention is produced by a liquid deep aeration fermentation method. The above-mentioned fully-mixed raw soup is first placed in a bioreactor, the fermentation temperature is 30 "C-5 5 V, and the p H value is adjusted to 4.0-6 with lactic acid. 5, fermentation time 5-8 days, stirring 1 "2 times a day. 24 hours from the start of fermentation, a mixture of 1-2% of the basidiomycete puree and / or seaweed puree is added at intervals of 24 hours.

6. Centrifugal waste residue: ■ ',

The fermented mixture was placed in an ultracentrifuge, and the temperature of the rotor was controlled at zero. -5 · (: between, centrifugation time 10 minutes / times, take the clear wave, centrifuge the slag plus lactic acid to extract for 24 hours and ultracentrifuge again to get the clear wave, combine the original supernatant after the second supernatant centrifugation Dregs can be used as feed additives.

7. Adsorption and decolorization:

The two obtained supernatants were adsorbed with a pore size 18 λ-2 A activated carbon, and unsaturated fatty acids were removed and decolorized.

8. Concentration:

The decolorized supernatant water is concentrated by a dehydrator to a gelatinous body with a combined water content of 30-35%.

9. Drying:

Put the above gel in a temperature-controlled drying oven to dry, the temperature should be controlled between 5 5-7 5 ^e C Dry for 4 hours to get the water content to 5. "8% powder finished product.>,

1 0. Finished product packaging:

The finished powder product is vacuum packed into bottles at 10 mg / bottle.

The preparation method of the present invention is described below through specific examples. Best Mode for Carrying Out the Invention Reference Example 1

Preparation of alpha-amylase

Corn starch 24 corn syrup 26 ¼ disodium 37 *, calcium chloride 1 potassium fluoride 0.2¾, magnesium chloride 0.2%, bran, red yeast rice 1% (the percentage of which is weight percentage), the pig pancreas is aerated and fermented The fermentation time is ⁷² hours, the temperature is ³⁷ ± l ^e C, and the pH value is 6.0-7.0. The culture product contains 30% of the dried amylase. `` Reference Example 2 ''

Preparation of fungal protease

Take corn starch 30, corn slurry 5 *, soy flour 5¾, casein gelatin 5%, dry sprinkler 5%, potassium dihydrogen phosphate 2.4%, sodium nitrate 1 ammonium fluoride 1%, iron ferrite 0.01 bran 5%, Fine bran 5 Ganoderma lucidum spore powder, red yeast rice 1 * (the percentage of which is weight percentage) is aerated fermentation, the fermentation time is 48 hours, the temperature is 34 ± 1 "C, and the pH value is 6.0-7.0. The culture product contains fungi 30% crystallization of protease.Reference Example 3-A

Preparation of cellulase

Take edible fungus green waste fungus material 65 bran 10¾, fine bran 10 *, ammonium sulphate 2.5¾, Yong 250%, yeast # 1%, Tween 80 0.05%, crude brown sugar 1 *, red yeast rice 1 * (of which The percentage is weight percentage) of solid fermentation. The fermentation time is 144 hours, the temperature is ⁴⁰ ± 1'C, and the pH is 4.0-5.0. The total amount of cellulase in the culture product is 4-5% dry weight. Reference example 3 -B

Preparation of cellulase

Take rice bran 1 bran 0.5 ammonium laurate 0.2%, dimethyl phosphate 0.1¾, calcium carbonate 0.1%, glucose

1% (wherein the percentage is weight percentage) in a Erlenmeyer flask, 30 ° C, 104 rpm, culture 32-35 hours The first-stage fermentation product of the seed is obtained; the waste fungus material ⁶ bran 0, ⁵ glucose 1 ammonium luteate ο · ² potassium dihydrogen phosphate 0.1 * at 30 · (:, 104 rpm, culture ⁶⁰ hours, and Seed secondary culture; seed primary, secondary culture, peptone 0.07.5 Tween 80 0.2, ammonium sulphate 1.4g / I dipotassium phosphate Og / I ^ urea o. 3g / i calcium carbonate o ³ g / ∑s magnesium sulphate Q.¾ / i iron osmate ^5. ogA sulphate i. ⁶ g / L, zinc osmate 1.4 g / cobalt gasification 2.0 g / Lj and polypropylene glycol with molecular weight 2000 the product as body waves defoamer for fermentation; wherein the amount of cellulase together is ^⁶³ _ ^7.0% dry weight reference Example 4.

Preparation of pectinase

Take pectin (carrot, mandarin orange, eggplant, apple, banana and other fruit juices) 4 peptone 0.1, sodium gallate 0.05¾, iron sulphate 0.01%, magnesium gallate 0.05¾, potassium fluoride 0.05¾, dihydrogen phosphate Potassium 0.1%, bran 5%, fine bran 5 sucrose 2 ammonium nitrate 0.2 sodium nitrate 0.2% (wherein the percentage is weight percentage), and connected with ² × 10 ³ spores of ganoderma lucidum in suspension waves, 30 ^e C Fill a 10 O ml Erlenmeyer flask

30 ml for 7 days. The total amount of pectinase in the obtained culture product is 15-20%. Example 1.

Take basidiomycetes and seaweed: 440 g of Lentinus edodes, 110 g of Flammulina velutipes, 110 g of Poria cocos, 20 g of Ganoderma lucidum, 2 2 O.g of kelp;

Chinese herbal medicines: Bo Ziren 230 g, Snake Bed 110 g, Cuscuta 110 g., Raspberry 110 g, Dendrobium 75 g, Morinda officinalis 45 g, Eucommia 90 g, Asparagus 9 0 g, Polygala spp. 70 g, Zhiren Kernel 15 g, Continuity 1 45 g, Black medicine 30 g, Calamus 40 g, Alisma 30 g, Yam 40 g, Ginseng 40 g, Rehmannia glutinosa 60 g, mulberry parasitic 20 g, dogwood 30 g, schisandra 12 g, stalactite 40 g, cistanche 90 g, oxgrass 20 g;

Auxiliary ingredients: Yeast * 5.5 g, Peptone 5.5 g, Brown sugar 1 37 · 5 g, Shi Yi powder 1.35 g, KC1 1.3 g, MgSO ₄ 0 .275 g, KH ₂ P0 ₄ 0 .2 75 gg, (HJ ₂ S0 ₄ 0 .8 2 g;

Take the cellulase of Reference Example 3-A of oxalase 0: 0 8 7 g;

Flammulina velutipes puree 0-8 7 g; mushroom puree 0.43 5 g; puree of ganoderma and kelp (1: 1) 6.5 g.

Crush the fungus and seaweed into powder, mix and pulverize the Chinese herbal medicine into 1 8 mesh fine powder, first soak the Chinese herbal medicine in a 159 & milk solution for 24 hours; then add the edible fungus and kelp powder, mix and stir well Then add auxiliary raw materials yeast peptone, peptone, brown sugar, stone powder, KC1, Mg SO

4, H ₂ P, (NH ^) 2 SC, etc. were thoroughly mixed and stirred for 8 minutes. Put the above mixture Placed in the bioreactor after sterilization, the enzyme was added oxalic acid bacteria fermentation with lactic acid to adjust the PH value of 5, aeration culture, the fermentation temperature is 40 fermentation time was 6 days, ² times a day with stirring, in 24 hours after the start of fermentation contact Add 2% of the enoki mushroom puree to the formula, and add 2% of the mushroom puree to the formula during 48 hours of fermentation, and add 1 to 5% of the formula's total weight within 7 to 2 hours of fermentation. Puree of Ganoderma and Mushroom. The fermented mixture was centrifuged in an ultra-centrifuge centrifuge at 2 'C for 10 minutes, and the clear water was taken. The centrifuged residue was leached with lactic acid for 24 hours. The centrifuge was centrifuged for 10 minutes and combined twice. The clear solution is adsorbed with activated carbon with a pore size of 18 A to remove the unsaturated medium fat. After the fat is decolorized, it is concentrated by a dehydrator to a colloid with a water content of 35%, and then placed in a temperature-controlled drying box at about 60 C. Dry for 4 hours to make 2 kg of powdered finished product with a total volume of 6%. Example 2

Take basidiomycetes and seaweed: 70 g of Lentinus edodes, 60 g of Flammulina velutipes, 10 g of Poria cocos, 30 g of Ganoderma lucidum, and 70 g of kelp;

Take Chinese herbal medicines: Bozi Ren 40 g, 床 bed 45 g, Cuscuta 40 g, Raspberry 45 g, Dendrobium 10 g, Morinda officinalis 15 g, Eucommia 10 g, Asparagus 15 g, Zhi Far (heart-free) 10 g, Noir kernel 5 g, Intermittent 5 g, Black medicine 7 g, Acorus 10 g, Alisma 7 g, Yam 10 g, Ginseng 8 g, Rehmannia glutinosa 8 g, Dogwood 6 g, 30 g of five gardenia seeds, stalactite (refined) 1 2 g, cistanche 2

0 g;

Auxiliary ingredients: 70 g of bran, 80 g of fine bran, 40 g of sweet potato powder, 12 g of yeast, sucrose

10 g, calcium carbonate 12 g;

3 g of cellulose ringworm made from Reference Example ³ -B

The mixture used to inoculate the basidiomycete puree and / or seaweed puree is: 0.16 g puree of mushrooms, 0.08 g of puree of mushrooms, ganoderma and kelp (1: 1) puree 0. 0 9 g.

The method of Example 1 was repeated to produce 300 g of a powder finished product with a water content of 6%.

This product treats kidney deficiency, liver stagnation and fire, and lack of temper. Example 3

Take basidiomycetes and seaweeds: 70 g of Poria cocos, 80 g of Ganoderma lucidum and 70 g of kelp;

Chinese herbal medicine: Polygonum multiflorum (manufactured) 50 g, Austrodia chinensis 40 g, Cuscuta chinensis 40 g, Ligustrum lucidum 40 g, Eucommia ulmoides 40 g, Agaricus syringa 60 g, Mulberry leaf 20 g, 40 g of silver flower, 40 g of black mulberry, 4 g of wolfberry, 60 g of chrysanthemum, 40 g of rehmannia glutinosa, 30 g of yam meat, 40 g of tannin, 4 g of diarrhea, and 40 g of yam;

Extraction of auxiliary raw materials: 70 g of bran, 80 g of fine bran, 12 g of yeast, 12 g of sucrose, calcium carbonate Take bacillary oxidase as the brazillin 'enzyme enzyme 0.2 g made in Reference Example 3-A and pectin Xue 0.1 g made in Reference Example 4;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: 0.16 g of mushroom puree, 0-16 g of mushroom puree, and 0 · 0 9 _{g of} kelp puree.

The method of Example 1 was repeated to prepare a powder finished product with a water content of 6 9 & 9 4 0 g.

This product can calm blood and nourish yin, dissipate blood stasis and clear collaterals, and lower blood lipids. Example 4

Take basidiomycetes and seaweed: 4.0 g of shiitake mushrooms, 0 g of mushrooms, 40 g of Hericium erinaceus, 60 g of kelp, and 40 g of seaweed;

Take Chinese herbal medicine: 100 g of yellow medicine, 50 g of chuanchuan, 50 g of shayuanzi, 40 g of oysters, 40 g of zedoary turmeric, 40 g of coix seed, 40 g of chuanxiongzi, 30 g of green peel,蜈蚣 6 bars, ¾6 bars, Qingshi 30 g, unicorn 30 g, Amomum villosum 20 g, chicken gizzard 20 g, codonopsis 20 g, astragalus 60 g, cooked rehmannia 60 g, Ergu 30 g, betel nut slices 4 δ g, Sichuan army 15 g, Yuanming powder 15 g, dried toad 8 g, acute child 10 g, Zhuru 20 g and vermiculite 45 g;

Take auxiliary materials:

45 g of bran, 45 g of fine bran, 15 g of yeast, 35 g of brown sugar, 3'5 g of soy flour, 10 g of calcium carbonate _;

Take bacterial oxalase as cellulose 0.2 g made in Reference Example 3-A and fungal protease 0.1 g made in Reference Example 2;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: 0.2 g of mushroom puree, kelp puree 0.1 g, ganoderma puree 0,, 1 g _?.

The method of Example 1 was repeated to produce a finished product of 6% of the total volume of 11.8 g.

This product treats liver cancer and pancreatic head cancer. Example 5

Take basidiomycetes and seaweeds: Tremella 100 g ·, shiitake mushrooms 15 g, lettuce 50 g;

Take Chinese herbal medicine: 30 g of black ginseng, 30 g of Ophiopogon, 30 g of Rehmannia glutinosa, 20 g of red peony,.

0 g, astragalus 30 g, yam 30 g, coix seed 20 g, safflower 15 g, hoehu 5 g and sea cucumber 7

5 g;

Take auxiliary materials: bran 8 0 g, polishings 7 0 _g, sweet potato powder 8 0 g, yeast 1 5 _g, sucrose 20 g, calcium carbonate 10 g;

Take bacillary oxidase as reference enzyme 0.18 g made in Reference Example 3-A and pectinase 0.01 g made in Reference Example 4;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: lenticular puree 0.16 g, kelp puree 0. 8 g, and ganoderma puree 0. 9 g.

The method of Example 1 was repeated to produce a finished product 715 g with a water content of 6%.

This product can be used to treat middle and advanced stages of diabetes. . Example 6

Take Basidiomycetes and seaweed: 30 g of Lentinus edodes, 20 g of Poria, 50 g of Poria, 50 g of kelp; Chinese herbal medicine: 20 g of Astragalus, 20 g of Codonopsis, 20 g of Fangshen, 2 0 of Atractylodes g, Shouwu 3

0 g, Alisma 40 g, Hawthorn 40 g, Yin Chen 40 g, Buffalo Horn 30 g, Epimedium 30 g and Rhubarb 6 g;

Take the auxiliary raw mash: 100 g of bran, 60 g of fine bran, 60 g of sweet potato flour, 12 g of baker's yeast, 12 g of brown sugar, and 12 g of calcium carbonate;

Take the bacterial oxalase as the fiber f enzyme 0.18 g made in Reference Example 3-A and the fungal protease 0.08 g made in Reference Example 2;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: 0.15 g of mushroom puree, 0.08 g of mushroom puree, and 0.99 g of ganoderma puree.

The method of Example 1 was repeated to obtain a finished product of ^{6 8 5} g with a hydration content of ⁶ %.

This product can treat simple obesity ^ «Example 7

Take basidiomycetes and seaweed: 200 g of shiitake mushrooms, 100 g of kelp _;

Chinese herbal medicine: Polygonum multiflorum 220 g, Ligustrum lucidum 100 g, Huai yam 100 g, Mulberry 50 g and Hangbai chrysanthemum 40 g;

Take auxiliary materials: 120 _g of bran, 60 _g of fine bran, 12 g of yeast, 20 g of brown sugar, 12 _g of calcium carbonate;

Bacterial oxidase was used as reference ^ vitamin E enzyme 2 · 2 g made in Reference Example ³ -B and α amylase 0.1 g made in Reference Example 1;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: 0.16 g of raw oyster puree, 0.08 g of kelp raw puree, and 0.08 g of raw mushroom puree. The method of Example 1 was repeated to obtain a finished product of 9 8 8 g with a water content of ⁶ %.

This product has the functions of nourishing blood, nourishing liver, nourishing sage, and promoting hair growth. It is used for baldness, alopecia areata, alopecia areata, and young white hair. Industrial applicability

The invention has the following characteristics, and thus can have a wide range of applications:

1. The persuasive health care medicine prepared by the method of the present invention is combined with bacterial protein, seaweed single-cell soluble protein, active ingredients of Chinese herbal medicines, and trace nutrients. Nutrient elements. It is composed of small molecule proteins with strong cell activity. And peptide substances, can penetrate the brain barrier and cell membranes, reach diseased areas, and kill disease factors and viruses. Clinically proven, this drug has a significant effect on the treatment of diseases such as aging and aging, and can delay the aging effect. The persuasion, health-preserving medicine of the present invention has the obvious persuasion effect that the curative effect is good and the effect is fast compared with the similar formula and the medicine made by other processing methods.

2. The comprehensive treatment of this health-care medicine is good, it is easy to be compatible with other medicines, and it can strengthen the treatment of disease and disease.

3. The total amount (weight percentage) of various components measured in the health medicine prepared by the method of the present invention is: protein 10.046¾ fat 2.843 *

Pseudomonas polysaccharide 40 · 5¾ Vitamin 4.04 *

Free Amino Acids 8.8 *

Of which (Unit: mg / 100g):

Isoleucine 0.342 Leucine 0.6

Lysine 0.57 phenylalanine 0.345 threonine 0.42 succinic acid 0.996 tyrosine 0.27 alanine 0.594 arginine 0.288 aspartic acid 0.75 serine 1.492 cystine 0.042 glycine 0.324 tryptophan 0.082 glutamic acid 0.81 glutamic acid 0.408 methionine 0.054 proline 0.423 element (mg / lOOg)

Potassium 289 硪 24

Sodium 2.3 Magnesium 26

Calcium 1177 Phosphorus 110

Iron 150 Vitamin (mg / 100g)

Carotene 0.1 riboflavin 0.13

Leucine 0.03 niacin 0.9

Cobalamin • 0.81 Ascorbic acid 0.19

Amino acid germanium, Oppm / ipog,. ■.

Amino acid selenium 250ppm / 100g

As well as a small amount of polyphenol hydratase, mannan, phytic acid, 1, 3-B-glucosaminidase, plant extracts, fermentin, fucoidan, etc. The total amount of heavy genus such as cadmium, mercury, and lead in biological fermentation products is within the allowable range.

4, the powders made according to the invention fermentation gavage to rats can not be measured LD _5. . Acute toxicity test is used to determine the maximum tolerated amount. For the test method, please refer to "New Chinese Medicine and Clinical Pharmacology" prepared by the Compilation Group of "Guidelines for the Study of Toxicology of New Chinese Medicine"1993; 4 (3): 13 Measures for the Examination and Approval of New Drugs, "Revisions and Supplementary Provisions on Chinese Medicine" 1992: 18. Taking the product of Example 1 as an example, 20 mice were used for experiments; the females were half prepared, and the highest concentration (28%) of the biological fermentation product of the present invention was 0.4ml / 10gBW. Once every 4 hours, 3 times in a row. Toxicity and death were observed at the end of 7 days. LD ₅ was calculated by intragastric administration of the biologically fermented mice of the present invention. More than 33.6g / kg, equivalent to 917 times the recommended clinical dosage. ''

5. For all kinds of diseases caused by visceral deficiency and yin-yang tone; It has 62 cases of sexually transmitted diseases and senile diseases. It is clinically applied in Example 2. The products are diagnosed according to the "Specifications for the Diagnosis and Treatment of Traditional Chinese Medical Diseases". The standard indicates that: the total effective rate for heart-yang deficiency (total 21 cases) is 95%; the total effective rate for heart-spleen deficiency (total 20 cases) is 90%; 1 case) with a total effective rate of 90; the total effective rate with respect to life-gate fire decay (a total of 10 cases) was 92%.

6. The preparation process of the present invention is simple and easy, the equipment is simple, the original tin is easy to obtain, the cost is low, and it is convenient for popularization and application.

Claims

1 A fermentation product, which has, engaged on the total weight of the main raw material 32-48 * basidiomycetes fruit bodies and seaweed, and the grass _ ⁵² ^ ⁶ J mainly original sound oxalate enzymes, as well as Enzyme-promoting agents, nitrogen sources, carbon sources, and nutritional supplements are the fermentation products of the original family, of which:

The weight ratio of basidiomycete to seaweed is 2: 0.5-1.5;

The seaweed escapes from one or two of kelp, seaweed, sea, and dogwood;

The bacterial oxalase is selected from one or more of amylase, cellulase, pectinase, and fungal protease, and the added amount is 0.01-0.03% of the total weight of the raw material;

The enzyme-promoting agent is selected from yeast * or corn slurry or a mixture thereof;

The nitrogen source and carbon source are selected from the group consisting of peptone, wheat husk, fine bran, sweet potato flour, corn flour, bran, rice bran, mashed potato flour, tulle powder, bean cake powder, peanut cake powder, rapeseed cake powder, One or more of black sesame powder, brown sugar and white sugar;

The nutritional additives are inorganic salts and minerals combined with phosphorus, calcium, f, thallium, selenium, and zinc; the weight ratio of the main original family and the enzyme-promoting agent, nitrogen source, carbon source, and nutritional additive is 100: 0.02 -0.1: 0.18-0.22: 4.6-5.4: 0.14-0.16.

2. The biological fermentation product according to claim 1, characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

7-8% shiitake mushrooms, 18-19 * mushrooms, Poria cocos 1-2¾, Ganoderma 2-3%, kelp 14-16%;

The Chinese herbal medicine has the following weight percentages:

Boziren 7-8%, Shezizi 3-4%, Cuscuta 3-4 Raspberry 3-4¾ί, Dendrobium 2-3 Morinda officinalis 1-2%, Continuity off 4-5 Black medicine 0.5-1, Calamus 1- 1.5 Alisma 0.5-1 yam 1-2 *, ginseng 1-2 cooked rehmannia 2-3 *, mulberry parasitic 0.5-1%, dogwood 1-1.5¾, five gardenias 3-5¾1, stalactites 1-2%, cistanche 2-3 * Wagyu 0.7-3;

The auxiliary raw material is a component having the following percentage of child weight:

Peptone 0.01-0.02¾, brown sugar 0.2-0.3 stone luxury powder 1%, KC1 0.4-0.5%, MgSO ₄ 0.4-0. 5%, H ₂ PO ₄ 0.4-0.5%, (HJ ₂ S0 ₄ 0.3-0.35% ₀

The bacterial oxalase is the cellulase.

3. The biological fermentation according to claim 1, which is characterized by:

The basidiomycetes and seaweeds have the following weight percentages: Shiitake Mushroom 7-8 *, Enoki Mushroom 4-6, Earth Poria 1-2, Ganoderma 2-3, Kelp 7-8%;

The Chinese herbal medicine has the following weight percentage components:

Bozi 4-5%, Cnidium ^{^4.4} ^4.5%, dodder ⁴ - ^⁴⁵ *, raspberry 4-4.5%, 1-1.5% Dendrobium, Morinda 1-1.5¾ί, gutta 1-1.5 Tianmen Winter 1-1.5¾ !, Zhiyuan (Go to heart) 1-1.5 *, Zhiren kernel 0.2-0.5%, On-off 0.5-0.7 *, Black medicine 0.5-0. 'Price, 0.7-1%, Ze Xie 0.7-1 yam 0.8-1 *, ginseng 0.8-1 cooked rehmannia 0.5-0.8 dogwood 0.6-1%, Schisandra 2-3 *, stalactite (refined) 1-1.2%, cistanche 2-3 <fe;

The auxiliary raw materials are components having the following weight percentages:

Bran 7-8%, fine bran 7-8%, ground flour yeast i-1.2%, sucrose 1-1.2%, calcium carbonate 1-1.2%.

4. The biological fermentation product according to claim 1, characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

Poria cocos 7-8%, Ganoderma lucidum 7-8%, with 7-8

The Chinese herbal medicine has the following weight percentage components;

Polygonum multiflorum (manufactured) 5-6%, pregnant with Achyranthes bidentata 3-4, Cuscuta 3-4%, Ligustrum lucidum 3-4%, Eucommia ulmoides Oliv. 4-5%, Corydalis 5-6 Mulberry leaves 2-3%, Silver flower 3-4 black mulberry 3-4%, Chinese wolfberry 0.3-0. 4%, chrysanthemum 5 -6%, radix rehmanniae 3-4 mangosteen meat 3-4%, tank 3-4 Alisma 0.3-0.4 yam 3- 4¾;

Bran 7-8 fine bran 7-8%, yeast 1-1.2 sucrose 1-1.2 calcium carbonate 1-1.2%;

The bacterial oxalase is 0.02 * cellulase and 0.01% pectinase.

5. The biological fermentation product according to Forklift claim 1, characterized in that:

The basidiomycetes and seaweeds have a weight percentage of Rugao:

Shiitake Mushroom 3-4 Mushroom 3-4%, Hericium Mushroom 3-4¾ ;, Kelp 5-6

The Chinese herbal medicine has the following weight percentage components:

Huangyaozi 10-20 *, Chuanchuan '4-5 <¾, Shayuanzi oysters 3-4%, Curcuma 3-4 *, Coix seed 3-4 Chuanxiongzi 4-5 *, Qingpi 3-4 蜈蚣 1,2 -1.5 *, cantharicus 1.2-1.5 <fe, talc 3-4 unicorn 3-, amomum 2-3 chicken gizzard 2-3 codonopsis 2-3 *, astragalus 3-6 cooked rehmannia 3-6% , Erhu 3-6%, betel nut slices 3-6%, Sichuan army l-2 <fe, Yuanming powder 1-2 dried toad 0 · 5-1 *, acute offspring 0.6-1.2%, Zhuru 2-3% and Substitute 4-5 *;

Bran 4-5%, fine bran 4-5 yeast 1. · 2-1.5%, brown sugar 3-4¾, soy flour 3-4%, calcium carbonate 1-1

.2%; The fungal oxalate is 0.02% of the above-mentioned cellulase and 0.01% of the above-mentioned pectinase.

6. The biological fermentation product according to claim 1, characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

Tremella 10-15 *, Shiitake Mushroom 1.5-4.5 'Hot Vegetables 5-8'

The Chinese herbal medicine has the following weight percentage components:

Scrophularia 3-4 *, Ophiopogon 3-4 *, Raw Dihuang 3-4 Chiba 1-2 Danpi 1-2 *, Astragalus 3-4 Yam 3-4%, Coix Seed 1-2 *, Coriander flower 1-2 Bupleurum 0.5-0.6¾; and sea cucumber 7-8 ^

The auxiliary raw material is a component having the following weight percentage:

Bran 6-8%, fine bran 6-8 sweet potato powder 6-8 yeast 1.2-2 総 1.2-2 *, calcium carbonate 1-1

.2%;

The bacterial oxalase is 0.02% of the aforementioned cellulase and 0.01¾ of the aforementioned pectinase.

7. The biological fermentation product according to claim 1, characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

Shiitake 2-3%, Poria 1-2 Poria 4-5 *, Kelp 4-5 *;

The Chinese herbal medicine has the following weight percentage components:

Astragalus 1-2%, Codonopsis 1-2 prevention has 1-2 0 wood 1-2 *, raw Shouwu 2-, Zexie 4-8 *, hawthorn 2-4%, Yin Chen 2-4 buffalo horn 2- 4¾, · Epimedium 2-4 * and rhubarb 0.6-1%;

10-12 * bran, fine bran ^{5 _6¾,} sweet potato powder 5-6¾, 1-1.2 * baker's yeast, brown sugar ^ 51-1.2 1-1.2% carbonate;

The bacterial oxalase is the above-mentioned fungal protease of o.im. i. Cellulase oo.01 *.

8. The biological fermentation product according to claim 1, characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

Shiitake 15-20 *, Kelp

The Chinese herbal medicine has the following weight percentages:

Polygonum multiflorum 18-22 *, Ligustrum lucidum 9-10 Huai yam 9-10 *, Mulberry 4-5¾ί and Hangbaiju 3-4¾ ;; The auxiliary primary family is a component having the following weight percentages:

Bran 10-12 *, fine bran 5-6%, yeast 1-1.2 *, brown sugar 1.5-2.5 *, calcium carbonate 1-1.2%; the bacterial oxalase is 0.02 * of the above-mentioned cellulase and 0.01 * Of the above amylase.

9. A method for preparing a biological fermentation product, comprising the following steps:

(1) Take the main drug family, which includes fungi and seaweeds with a weight ratio of 32 to 48, and Chinese herbal medicines with a weight ratio of 52 to 68, bacterial oxalase; obtain enzyme production promoters, nitrogen sources, carbon sources, and Nutritional additives as medicine Auxiliary department

(3) immerse the Chinese herbal medicine powder with 15 * concentration of lactic acid for 24 hours, and then add the crushed fungi and seaweed powder, mix and stir; add the above mentioned drug auxiliary raw materials, and stir thoroughly to obtain a mixed raw material to sterilize;

'(4) Put the mixed proteaceae and bacterial oxalase described in (3) in a bioreactor, adjust the pH to 4.0-6.5 with lactic acid at 30-55 ^e C, and culture for 5-8 days aeration, daily Stir 1-2 times, and start the fermentation on the 24th hour. Every 24 hours, a mixture of a basidiomycete puree and / or seaweed pulp is used. The number of inoculations is 2 -3 times to get fermentation product;

(5) centrifugally discarding the fermentation product, desorbing, concentrating, and drying to produce the product; wherein:

The weight ratio of basidiomycete to seaweed is 2: 0.5-1.5;

The basidiomycete is selected from one or more kinds of Lentinula edodes, Flammulina velutipes, Poria, Hodgkin, Ganoderma lucidum, Hericium erinaceus, mushroom, and Tremella;

The seaweed is selected from one or two of kelp, laver, seaweed, and hairweed;

The bacterial oxalase is selected from one or more of α-amylase, cellulase, pectinase, and fungal protease, and the added amount is the raw material. Total weight 0.01-0.0 ^;

The enzyme-promoting agent is selected from yeast or corn syrup or a mixture thereof;

The nitrogen source and carbon source are selected from the group consisting of peptone, wheat husks, fine bran, sweet potato flour, corn flour, bran, rice bran, potato flour, tulle powder, bean cake powder, peanut cake powder, rapeseed cake powder, and black sesame seeds. One or more of powder, brown sugar, and white sugar;

The nutritional additives are inorganic salts and minerals combined with phosphorus, calcium, potassium, thallium, selenium, and zinc; the weight ratio of the main raw material to the enzyme-promoting agent, source, carbon source, and nutritional additive is 100: 0.02- 0.1: 0.18-0.22: 4.6-5.4: 0.14-0.16.

1 0. The preparation method according to claim 9 is characterized in that:

The "-amylase is derived from corn starch 2, corn slurry 26¾Γ disodium hydrogen phosphate 37¾, calcium fluoride

1%, vaporized magnesium, potassium fluoride ^0.2 bran bed ο · ² ^ H Monascus culture product of a porcine pancreas;

The fungal protease is derived from corn starch 30 *, 'corn slurry 5¾, soy flour 5%, casein 6%, gelatin 5 *, dry sprinkler 5%, dimethyl phosphate 2.4%, sodium nitrate 1 ammonium chloride 1 ferric acid 0.01 bran 5%, fine bran 5 ganoderma spore powder 5 red yeast rice 1 * culture product;

The cellulase is derived from edible fungus green waste fungus 65%, bran 10%, fine bran 10%, ammonium sulphate 2.

5%, Yong 250%, Yeast 赍 1¾, Tween SO 0.05%, Crude Brown Sugar 1%, Monascus 1% solid fermentation product; Bran derived from rice bran, or a sulfonium, ammonium 0.5 0.2 0.1 dicalcium phosphate, potassium argon, calcium carbonate, 0.1, 1 * glucose in Erlenmeyer flask, 30-C, 10 ⁴ dtex / min, ³² _ culture ³⁵ hours to obtain a seed King Fermentation product; the waste fungus material, bran 0.5%, glucomannan 1 ammonium 0.2, potassium diargon phosphate 0.1 * at 30 · (:, 104 rpm, 60 hours of cultivation, and a seed secondary culture is obtained; seeds are one, two cultures, peptone, 0.075%, Tween 80 0.2, sulfur, ammonium l. ⁴ g / I potassium dihydrogen phosphate 2. Og / urea O.Sg/L^ gasification calcium 0.3g / I magnesium sulphate OJg / I iron sulphate S.Og / I ^ manganese sulphate l.Sg/ zinc sulphate lg / cobalt gasification 2. Og / L, and using polypropylene glycol with molecular weight 2000, The product of wave fermentation by Huaihuai; the pectinase is derived from 4% of pectin, 0.4% of sodium peptone, 0.05% of sodium plutonate, 0.01¾ of iron sulphate, 0.05¾ of magnesium sulphate, 0.05% of potassium fluoride , Potassium dihydrogen phosphate 0.1%, bran 5¾, fine bran 5%, sucrose 2 ammonium nitrate 0.2%, sodium nitrate 0.2 *, and connected with 2X10 ³ spores of Ganoderma spore suspension wave, 30'C, at The culture product obtained after 7 days of cultivation in an Erlenmeyer flask, where the percentage is The percentage amount.

1 1. The preparation method according to claim 10, characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

Shiitake Mushroom 7-8¾, Flammulina Mushroom 18-19¾, Poria 1-2¾, Poria 2-3¾, Kelp 14-16¾;

The Chinese herbal medicine has the following weight percentage components:

Boziren 7-8%, Snakebed ³ _ ⁴ ¾, Cuscuta ^3- ： Raspberry 3-4%, Dendrobium 2-3¾, Morinda officinalis 1-2¾, Continuity off 4-5%, Black medicine 0.5-1 Acorus 1-1.5¾, Alisma 0.5-1 yam 1-2%, Panax ginseng 1-2¾, Rehmannia glutinosa 2-3%, Morus alba 0.5-1 *, Fructus Corni 1-1.5, Fructus Corni 3-5¾, Stalactite 1- 2 Cistanche 2-3 * Wagyu 0.7-1

The auxiliary raw materials have the following weight percentages:

Peptone 0.01-0.02%, brown sugar 0.2-0.3%, stone powder 1 *, KC1 0.4-0.5%, MgSO ₄ 0.4-0. 5%, KH ₂ PO ₄ 0.4-0.5%, (H ₄ ) ₂ SO ₄ 0.3 -0.35%;

The bacterial oxalase is the prepared cellulase;

12. The preparation method according to claim 1, which is characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

Shiitake 7-8 Shiitake Mushroom 4-6¾, Poria cocos 1-2%, Ganoderma 2-3 Kelp 7-;

The Chinese herbal medicine has the following components by weight:

Bozi Ren 4-5%, Snake Bed 4-4.5¾, Cuscuta 4-4.5¾, Raspberry 4-4.5¾, Dendrobium 1-1.5 Morinda officinalis 1-1.5¾, Eucommia 1-1.5 *, Asparagus 1-1.5 *, Zhiyuan (Go to Heart) 1-1.5 Nootropic kernel 0.2-0.5%, On-off 0.5-0, 7 6, 10.5-0.7%, Acorus 0 · 7-1¾, Alisma 0.7-1%, Yam 0.8-1%, Ginseng 0. 8-1 *, Rehmannia glutinosa 0. 5-0. ⁸ ¾, Dogwood 0. 6-1¾ :, Five Gardenia 2-3¾, Stalactite (refining) 1-1. 2 *, Cistanche 2-3% ;

Bran 7-8%, fine bran 7-8%, sweet potato powder 4-4. 2 yeast 1-1.2%, sucrose 1-1.2 calcium carbonate 1-1.2%;

The bacterial oxalase is the cellulase;

The Basidiomycetes ϋ and / ¾ 海 ¾¾ for the inoculation are in the following mixture: enoki mushroom puree, mushroom puree, edible oyster and kelp puree with a weight ratio of 2: 1.

1 3. The preparation method according to the fork profit requirement 10, characterized in that:

The basidiomycetes and sea 'algae' have the following weight percentages:

Soil Poria 7-, 炅 7-8%, Kelp 7-8¾ _;; ,

The Chinese herbal medicine has the following weight percentage components;

Polygonum multiflorum (manufactured) 5-6 pregnant with achyranthes 3-4, Cuscuta 3-4, Ligustrum 3-4%, Eucommia ulmoides 4-5 *, Yarrow 5-6¾, mulberry leaf 2 3 *, silver flower 3-4 ¾, black mulberry 3-, wolfberry 0.3-0.4%, chrysanthemum 5-6%, Dihuang 3-4 mandrill meat 3-4 rambutan 3-Zixie 0.3-0.4%, yam 3-4%;

Bran 7-8 *, fine bran 7-8¾, yeast 1-1. 2¾, sucrose 1-1. 2 *, calcium carbonate 1-1. 2%;

The bacterial oxalase is 0.02% of the cellulase and 0.011 of the pectinase;

The mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake mushroom puree, mushroom puree, kelp puree. ''

14. The preparation method according to claim 1, which is characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

Shiitake 3-4 Mushroom 3-4, Hericium 3-4 kelp 5-6 *;

The Chinese herbal medicine has the following components:

Huangyaozi 10-20S ?, Chuandi 4-5 *, Shayuanzi 4-5 *, 'Oyster 3-4%, Curcuma 3-4%, Pick kernel 3-4%, Chuanxiongzi 4-5 Qingpi 3- 蜈蚣1.2-1.5 *, cantharidium 1.2-1.5%, talc 3- unicorn lotus 3-, amomum 2-3 *, chicken gold 2-3 <fe, codonopsis 2-3 *, astragalus 3-6%, mature land Huang 3-6¾ ;, Er Chou 3-6 Areca Nut Slice 3-6%, Sichuan Army 1-2 *, Yuanming Powder 1-2 Dried Toad 0. 5-1 *, Acute 0. 6-1. 2 Zhuru 2 -3 * 和代赭石 4-5 ^

Bran 4-5 Fine bran 4-5%, Yeast 1. 2-1. 5 Brown sugar 3-4 Soy flour 3-4 Calcium carbonate 1-1 The bacterial oxalate domain is 0.02 * of the ^ cellulase and 0.01 * of the pectinase;

The mixture used to receive the bascule f, ^ /. Or the sea and algae in this order are: oyster mushroom puree, kelp puree, and oyster puree. '

15. The preparation method according to claim 10 is characterized by:

The basidiomycetes and seaweeds have the following weight percentages:

Tremella 10-15 *, oyster mushroom 1. 5-4. 5 *, lettuce 5-8%;

The Chinese herbal medicine has the following groups of weight fractions:

Scrophularia 3-4%, Ophiopogon 3-4%, Raw Rehmannia 3-4 Chiba 1-2 Red Bark 1-2%, Astragalus 3-4S ;, Yam 3-4%, Coix Seed 1-2 Safflower 1-2 *, Bupleurum 0. 5-0. 6% and sea cucumber 7- 8

Bran 6-8 Fine bran 6-8%, Ground powder 6-8Ferment per 1.2-2%, Sucrose 1.2-2 Calcium carbonate 1-1

. 2%;

The bacterial oxalase is 0.02% of the cellulase and 0.01 * of the pectinase;

The mixture of the basidiomycete puree and / or seaweed puree used for inoculation is: shiitake mushroom puree, kelp puree, and ganoderma puree. ,,

16. The preparation method according to claim 10, characterized in that:

The basidiomycetes and seaweeds have the following weight percentages:

Shiitake 2-3, Poria 1-2%, Poria 4-5 Kelp 4-5

The Chinese herbal medicine has the following weight percentage components:

Astragalus 1-2 *, Codonopsis 1-2 *, Fangshen 1 ^: 2%, Atractylodes 1-2 *, Shengshouwu 2-4 Alisma 4-8%, Hawthorn 2-4%, Yinchen 2-buffalo horn 2-4%, Epimedium 2-4 * and Rhubarb 0.6-1 / 2;

Bran 10-12 *, fine bran 5-6 sweet potato flour 5-6%, baker's yeast 1-1.2%, brown sugar 1-1. 2¾, calcium carbonate 1-1. 2 *;

The bacterial oxalase is 0.02 * of the cellulase and 0.011 * of the fungal protease; the mixture of the basidiomycete puree and / or seaweed pulp for inoculation is: Puree, mushroom puree, laver puree ₀ ''

17. The preparation method according to claim 9 or 10, characterized in that:

The basidiomycetes and algae have the following weight percentages:

15-20% shiitake mushrooms, -10% kelp;

The Chinese herbal medicine has the following weight percentage components:

Polygonum 18-22¾, Ligustrum ^9-10 Chinese yam Mulberry ^9.10 ^{^4--5%} 3-4 * and Chrysanthemum; The auxiliary original family is a component having the following weight percentage:

Bran 10-12¾, fine bran 5-6 yeast 1-1. 2 brown sugar 1. 5-2. 5 calcium carbonate

The bacterial oxalase is 0.02 * of the cellulase and 0.1 of the amylase;

"The mixture of basidiomycota and / or sea syrup used for inoculation is: