CN102488253B - Preparation method of active seaweed extract - Google Patents
Preparation method of active seaweed extract Download PDFInfo
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- CN102488253B CN102488253B CN2011104464853A CN201110446485A CN102488253B CN 102488253 B CN102488253 B CN 102488253B CN 2011104464853 A CN2011104464853 A CN 2011104464853A CN 201110446485 A CN201110446485 A CN 201110446485A CN 102488253 B CN102488253 B CN 102488253B
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Abstract
The invention provides a preparation method of an active seaweed extract. The method comprises the steps of: seaweed selecting, impurity removing, weak acid washing, mincing and homogenizing, curing and sterilization under high temperature and high pressure as well as polysaccharide component degradation, pH adjustment, probiotic fermentation, and adding of alginate oligosaccharide for blending. The obtained liquid product is an active seaweed extracting solution, or it can be prepared into active seaweed extract powder through drying, and the extracting solution and the extract powder are collectively called an active seaweed extract. The active seaweed extract prepared in the invention reserves algae iodate, mannitol, inorganic salts, trace elements and other components, and the protein and polysaccharide components in seaweeds are degraded and easy to absorb. Added with probiotics, secondary metabolites, and low molecular weight seaweed polysaccharide, the active seaweed extract of the invention has better biological activity, and can remove harmful heavy metals so as to improve the security of the seaweed extract.
Description
Technical field
The present invention relates to the marine algae extract field, relate in particular to a kind of preparation method of active alga extract.
Background technology
Brown alga is the main component in the sea-plant.Sea-tangle in the brown alga, kelp, undaria pinnitafida, the sheep all edibles and medicinal such as dish of dwelling; Capable of using as feed or the fertilizer of Sargassum plant.In addition, also can from the brown algas such as sea-tangle, extract food or the pharmaceutical raw materials such as algin, sweet mellow wine, iodine, potassium chloride, laminaran.
Contain abundant nutriment and active material in the brown alga, also contain the nutritional labelings such as vitamin A, B, C and trace elements of selenium, cobalt, chromium, brown alga also contains a large amount of non-organic compounds containing nitrogens and unknown somatotropin simultaneously.Contained mineral matter and trace element exist mainly with the form of organic in the brown alga, are difficult for oxidizedly, are convenient to animal and absorb, and its effect and animal absorbability are better than inorganic mineral; The aliphatic acid that it contains is most of for unrighted acid, is mainly the essential fatty acids such as leukotrienes, inferior leukotrienes and eicosapentaenoic acid (EPA).Wherein sea-tangle is to contain one of the abundantest food of iodine on the earth, and seawood meal can directly be added in the food and feed as propiodal, as nutritious supplementary pharmaceutical.
At present, the form processing to edible brown algas such as sea-tangle, undaria pinnitafidas on the market has drying, salt marsh etc., both has been convenient to long-time preservation, increases again mouthfeel.In addition, the common simple form processing of sea-tangle is for making Kelp Powder.The technological process of Kelp Powder is: raw material sea-tangle → wash → soak → take off raw meat → airing → chopping → drying → pulverize → sieve → the finished product Kelp Powder.Whole process is just carried out the pulverizing variation of physics to sea-tangle, washing can be dispelled water-soluble inorganic arsenic, but can not dispel the heavy metals such as lead, cadmium, and algal polysaccharides wherein is to exist with the macromolecule state, can only be as common dietary fiber, not only can not be absorbed, but also affect the utilization rate of other active ingredients.
The preparation of Chinese invention patent 200610154800.4 water soluble seaweed powders.This method by enzymolysis, add the seawood meal that alkali transforms preparation and can in water, dissolve.But in long process, the loss by dissolutions such as the algin on sea-tangle surface, sweet mellow wine, water miscible vitamin, mineral matter lose the active material in the seawood meal greatly, and not degraded of algal polysaccharides wherein, be the macromolecule attitude still, be difficult to be absorbed.
Summary of the invention
For the defective that active component in the marine algae extract in the prior art often runs off greatly, the invention provides a kind of preparation method of active alga extract.The active alga extract of the present invention preparation has kept the original nutritional labeling of marine alga, and polysaccharide component wherein obtained effective degraded, by adding probiotics fermention, makes it have better biologically active.
For solving the problems of the technologies described above, the present invention is achieved by the following technical solutions:
A kind of preparation method of active alga extract, described method comprises the steps:
(1) selects the marine alga that clean and tidy nothing is gone rotten, reject assorted algae and foreign matter;
(2) dilute acid soln of the marine alga after the above-mentioned removal of impurities with PH2-4 cleaned, rubs, and in the solution of the diluted acid of PH2-4, grind homogenate;
(3) with the marine alga after the homogenate, 121～135 ℃ of temperature, the slaking sterilization is 0.5～2 hour under pressure 1～5MPa, the degradation of polysaccharide constituents;
(4) the marine alga slurries after will degrading add alkali and adjust PH to 5.0-7.0;
(5) inoculation cultured probio bacterial classification normal fermentation in advance in the marine alga slurries of adjusting behind the PH;
(6) add algin oligosaccharide in the slurries after the fermentation and allocate, probio is protected, breeds, the slurries after the allotment namely can be used as active alga extract product, but or drying make active alga extract powder product.
Further improvement to technical scheme: marine alga is dwell in dish, kelp or the undaria pinnitafida one or more of sea-tangle, sheep in the brown alga in the described step (1).
Further improvement to technical scheme: dilute acid soln is one or more in sulfuric acid, nitric acid or the acetic acid in the described step (2).
Further improvement to technical scheme: cleaning way is for embathing in the described step (2); The dilute acid soln quality is 3～10 times of marine alga quality; The time of embathing is 0.5～2 hour.
Further improvement to technical scheme: in the described step (2) during homogenate the quality of used dilute acid soln be 8～12 times of marine alga.
Further improvement to technical scheme: to adjust used alkali be in ammoniacal liquor, potassium hydroxide, potash or the calcium hydroxide one or more to PH in the described step (4).
Further improvement to technical scheme: probio is Bifidobacterium and/or lactic acid bacteria in the described step (5).
Further improvement to technical scheme: the inoculum concentration of probio is 2～5% of marine alga slurry volume in the described step (5).
Further improvement to technical scheme: described allotment is (2～5) with the quality of algin oligosaccharide and the mass ratio of fermentation rear slurry: 100.
Further improvement to technical scheme: described algin oligosaccharide is 10% algin oligosaccharide of 100～700mpa.s.
Compared with prior art, advantage of the present invention and good effect are:
1, the present invention adopts the weak acid scrubbing marine alga, when reaching clean result, the water soluble ingredient such as part inorganic salts, sweet mellow wine and the harmful heavy metal ions such as arsenic, Qian, Chrome are dispelled, and wherein the inorganic iodine part is dispelled, but organic iodine is retained in the frond fully; High temperature high pressure process after the acidic treatment can thoroughly be killed obstinate bacterial spore, and the polysaccharide composition in the degraded frond is conducive to the fermentation of follow-up probio.
2, the present invention also adds algin oligosaccharide in marine algae extract, can effectively protect probio; And algin oligosaccharide itself also is a kind of active material with unique physiological function.
3, the active alga powder of the present invention's preparation has the function of oligomeric algal polysaccharides, is easy to absorb, and utilization rate is high.
The specific embodiment
The present invention is further detailed explanation below in conjunction with the specific embodiment.
Embodiment 1
The preparation method of a kind of active alga extract of the present invention may further comprise the steps:
(1) selects the sea-tangle 1kg that clean and tidy nothing is gone rotten, reject assorted algae and foreign matter;
(2) take by weighing the 5Kg running water, adjusting PH with sulfuric acid is about 3, sea-tangle is immersed to stir embathe 1 hour;
(3) after the sea-tangle after will cleaning is rubbed, add PH and be 3 together homogenate of dilute sulfuric acid 8kg;
(4) with gained kelp paste after the homogenate, under 125 ℃, 3 MPa, kept 1 hour slaking sterilization and degradation of polysaccharide constituents;
(5) the algae slurries after will degrading add ammoniacal liquor and adjust PH to 6.5;
(6) in the marine alga slurries after adjusting PH, by volume 3% inoculation in advance cultured bifidobacterium species under 37 ℃ of conditions, anaerobic fermentation 24 hours;
(7) add the 0.3Kg algin oligosaccharide in the slurries after the fermentation and allocate, probio is protected, breeds;
(8) allotment after slurries namely can be used as active alga extract product, but or drying make active alga extract powder product.
Embodiment 2
The preparation method of a kind of active alga extract of the present invention may further comprise the steps:
(1) select the sea-tangle 30kg that clean and tidy nothing is gone rotten, the sheep dish 70kg of dwelling rejects assorted algae and foreign matter;
(2) take by weighing the 600Kg running water, adjusting PH with nitric acid is about 3, sea-tangle is immersed to stir embathe 1.5 hours;
(3) after the sea-tangle after will cleaning is rubbed, add PH and be 3 together homogenate of rare nitric acid 800kg;
(4) with homogenate gained kelp paste, under 125 ℃, 3 MPa, kept 1 hour slaking sterilization and degradation of polysaccharide constituents;
(5) the algae slurries hydro-oxidation potassium after will degrading is adjusted PH to 6.5;
(6) in the marine alga slurries after adjusting PH, by volume 4% inoculation in advance cultured lactic acid bacteria bacterium bacterial classification under 37 ℃ of conditions, anaerobic fermentation 24 hours;
(7) add 40 kilograms of algin oligosaccharides in the slurries after the fermentation and allocate, probio is protected, breeds;
(8) allotment after slurries namely can be used as active alga extract product, but or drying make active alga extract powder product.
Embodiment 3
To test by above-mentioned steps with the sea-tangle of embodiment 2 equals in quality, quantity, wherein only will wash, homogenate time institute adds diluted acid and is replaced by running water, carries out check experiment, then the marine alga slurries after the HTHP degraded, after adjusting PH to 6.5 with ammoniacal liquor, specifically to such as table 1.
Table 1: the acid condition degradation effect table of comparisons.
| Comparative run | Add running water washing, homogenate, degraded | Add weak acid scrubbing, homogenate, degraded |
| Outward appearance | Viscous pastes | Weak solution |
| Viscosity | 1200mPa.s | 10 mPa.s |
| Operability | Can't carry out rear operation inoculation, fermentation should not be carried out again | The water-based feed liquid that viscosity pole is low, operability is good |
Above embodiment is only in order to illustrating technical scheme of the present invention, but not limits it; Although with reference to previous embodiment the present invention is had been described in detail, for the person of ordinary skill of the art, still can make amendment to the technical scheme that previous embodiment is put down in writing, perhaps part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the spirit and scope of the present invention's technical scheme required for protection.
Claims (9)
1. the preparation method of an active alga extract is characterized in that described method comprises the steps:
(1) selects the marine alga that clean and tidy nothing is gone rotten, reject assorted algae and foreign matter;
(2) dilute acid soln of the marine alga after the above-mentioned removal of impurities with PH2-4 cleaned, rubs, and in the solution of the diluted acid of PH2-4, grind homogenate;
(3) with the marine alga after the homogenate, 121～135 ℃ of temperature, the slaking sterilization is 0.5～2 hour under pressure 1～5MPa, the degradation of polysaccharide constituents;
(4) the marine alga slurries after will degrading add alkali and adjust PH to 5.0-7.0;
(5) inoculation cultured probio bacterial classification normal fermentation in advance in the marine alga slurries of adjusting behind the PH, described probio is Bifidobacterium and/or lactic acid bacteria;
(6) add algin oligosaccharide in the slurries after the fermentation and allocate, probio is protected, breeds, the slurries after the allotment namely can be used as active alga extract product, but or drying make active alga extract powder product.
2. the preparation method of a kind of active alga extract according to claim 1 is characterized in that marine alga in the described step (1) is dwell in dish, kelp or the undaria pinnitafida one or more of sea-tangle, sheep in the brown alga.
3. the preparation method of a kind of active alga extract according to claim 1 is characterized in that the middle dilute acid soln of described step (2) is one or more in sulfuric acid, nitric acid or the acetic acid.
4. the preparation method of a kind of active alga extract according to claim 1 is characterized in that cleaning way is for embathing in the described step (2); The dilute acid soln quality is 3～10 times of marine alga quality; The time of embathing is 0.5～2 hour.
5. the preparation method of a kind of active alga extract according to claim 1, the quality of used dilute acid soln is 8～12 times of marine alga when it is characterized in that homogenate in the described step (2).
6. the preparation method of a kind of active alga extract according to claim 1 is characterized in that it is in ammoniacal liquor, potassium hydroxide, potash or the calcium hydroxide one or more that PH in the described step (4) adjusts used alkali.
7. the preparation method of a kind of active alga extract according to claim 1 is characterized in that the inoculum concentration of probio in the described step (5) is 2～5% of marine alga slurry volume.
8. the preparation method of a kind of active alga extract according to claim 1 is characterized in that described allotment is (2～5) with the mass ratio of the quality of algin oligosaccharide and fermentation rear slurry: 100.
9. the preparation method of a kind of active alga extract according to claim 1 is characterized in that described algin oligosaccharide is 10% algin oligosaccharide of 100～700mpa.s.
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| CN105647229B (en) * | 2016-01-21 | 2017-08-29 | 王君华 | A kind of state of aggregation natural dye based on marine alga gel and preparation method thereof |
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Application publication date: 20120613 Assignee: Jiangsu Mingyue Marine Biotechnology Co., Ltd. Assignor: Qingdao Moon Marine Technology Co., Ltd. Contract record no.: 2013370000259 Denomination of invention: Preparation method of active seaweed extract Granted publication date: 20130130 License type: Common License Record date: 20131206 |
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Effective date of registration: 20170713 Address after: 266400, Mingyue Road, Huangdao District, Shandong, Qingdao 777 Patentee after: Qingdao Bright Moon Seaweed Group Co., Ltd. Address before: 266400 Qingdao, Shandong Province, the city of Jiaonan Road, No. 67 Patentee before: Qingdao Moon Marine Technology Co., Ltd. |
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