CN107510042A - Seaweed nutritive liquid and preparation method thereof - Google Patents
Seaweed nutritive liquid and preparation method thereof Download PDFInfo
- Publication number
- CN107510042A CN107510042A CN201710693003.1A CN201710693003A CN107510042A CN 107510042 A CN107510042 A CN 107510042A CN 201710693003 A CN201710693003 A CN 201710693003A CN 107510042 A CN107510042 A CN 107510042A
- Authority
- CN
- China
- Prior art keywords
- seaweed
- liquid
- enzymolysis
- preparation
- marine alga
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The invention discloses a kind of seaweed nutritive liquid and preparation method thereof, procedure includes：Marine alga raw material is obtained, and carries out processing in small, broken bits；Obtained again with tune pH value to neutrality after sodium pyrophosphate predigestion, the ultrasonic enzymolysis of complex enzyme, sterilizing, finally composite bacteria fermentation process, addition mannitol and Function of Vitamin-E balance and accelerator.The above-mentioned seaweed nutritive liquid of the present invention, compound nutritional composition therein is utilized using marine alga comprehensively as raw material, by digesting, fermenting, the processing step that composition is compound, the macromolecular substances being difficult by marine alga are degraded to micromolecular polysaccharide, monose, nitrogen-containing compound, and substantial amounts of beneficial bacterium and a variety of special efficacy metabolites are produced, the composition collaboration for addition of then arranging in pairs or groups again makes final nutritional ingredient effect be more conducive to play；And be finally prepared containing various compound amino acids, protein, algal polysaccharides natural nutrient solution, maximumlly improve the values of seaweed products.
Description
Technical field
The present invention relates to marine alga processing technique field, more particularly to a kind of seaweed nutritive liquid and preparation method thereof.
Background technology
Sea-plant is commonly called as marine alga, is resource valuable in ocean, the mankind are played with more and more important effect, marine alga
Application value be not only embodied on edibility, also in ecological environmental protection, marine drug, animal feed, biological active matter
Matter development and application, food additives, chemical industry, organic fertilizer, packaging material for food, microbiological culture media, cosmetics and life
The numerous areas such as the thing energy also play the effect to become more and more important, there is huge application potential and high economic value.In marine alga
Sulfated polysaccharide, dietary fiber, cellulose, amino acid, protein, peptides, chlorophyll, carrotene, carotenoid, nicotinic acid,
Folic acid, pantothenic acid, macroelement, trace element, iodine and enzyme etc. are the active materials in marine alga.
And for the utilization of current seaweed products, it is to be directly used as edible or be processed into complex food mostly；Other are few
Amount ground carries out biofermentation as raw material, in the extraction for producing a large amount of processing special components containing specific marine alga Metabolite,
It is relatively low for the utilization rate of the seaweed nutritive composition of totality, reduce the value of seaweed products.
The content of the invention
It is a primary object of the present invention to provide a kind of seaweed nutritive liquid and preparation method thereof, it is intended to maximumlly lifting sea
The value of algae product, extend its application effect.
To achieve the above object, seaweed nutritive liquid and preparation method thereof of the invention, including:
Marine alga raw material is obtained, and carries out processing in small, broken bits；
The sodium pyrophosphate of seaweed weight 0.1~2% is added into the marine alga after in small, broken bits and carries out predigestion in 55~60 DEG C,
Obtain predigestion slurries；
The predigestion slurries are digested under ultrasound condition with complex enzyme, obtain enzymolysis liquid；The complex enzyme includes accounting for
The Pomacea canaliculata digestive ferment of seaweed weight 0.1~0.5%, 0.1~0.2% circumscribed 1,4 beta-glucanase；
The enzymolysis liquid is heated into 85~90 DEG C keeps 10~25min to be sterilized；
Fermentation process is carried out by composite bacteria is added in the enzymolysis liquid after sterilizing, collects zymotic fluid；The composite bacteria by
Saccharomycete, bifidobacterium breve, lactobacillus plantarum composition；
Mannitol, 0.1%~0.4% vitamin E of zymotic fluid weight 2%~5% are added into the zymotic fluid, and adjusts pH value extremely
6.5~7.5.
Seaweed nutritive liquid prepared by the present invention, compound nutritional composition therein is utilized as raw material using marine alga comprehensively, passed through
Cross the compound processing step of digestion, fermentation, composition, by the macromolecular substances being difficult by marine alga be degraded to micromolecular polysaccharide,
Monose, nitrogen-containing compound, and substantial amounts of beneficial bacterium and a variety of special efficacy metabolites are produced, the composition association for addition of then arranging in pairs or groups again
It is more conducive to play with final nutritional ingredient effect is made；And finally it is prepared containing various compound amino acids, protein, sea
The natural nutrient solution of polysaccharides, have anticancer, hypotensive, norcholesterol, supplement mineral element, beauty, fat-reducing, anti-aging and
The effect of internal heavy metal ion is excluded, has embodied huge application potential and high economic value.
Embodiment
The realization, functional characteristics and advantage of the object of the invention will be described further in conjunction with the embodiments.It should be appreciated that this place
The specific embodiment of description only to explain the present invention, is not intended to limit the present invention.
The present invention provides a kind of preparation method of seaweed nutritive liquid, and method and step includes：
S10, select clean and tidy clean to marine alga without mildew and rot marine alga, the water washing by soaking of 5~15 times of volumes of addition；
S20, the marine alga progress after step S10 processing is in small, broken bits, to 2~5mm of particle diameter；
S30, after adding the sodium pyrophosphate of seaweed weight 0.1~2% into the marine alga after step S20 processing, and enter in 55~60 DEG C
Row predigestion, obtain predigestion slurries；
S40, predigestion slurries are subjected to depth digestion with complex enzyme, the complex enzyme used in the step includes seaweed weight ratio
After 0.1~0.5% Pomacea canaliculata digestive ferment, 0.1~0.2% circumscribed 1,4 beta-glucanase, enzymolysis, digestion is carried out in ultrasound field,
Enzymolysis 3~5h centrifugations remove precipitation and obtain enzymolysis liquid；
S50, step S40 enzymolysis liquid is heated to 85~90 DEG C and keeps 10~25min to carry out sterilization treatment；
S60, composite bacteria is added into the enzymolysis liquid after sterilizing and carries out fermentation process, collection zymotic fluid；
S70, after zymotic fluid centrifugation is discarded into precipitation, add mannitol, the life of 0.1%~0.4% dimension of zymotic fluid weight 2%~5%
After plain E, and it is seaweed nutritive liquid of the invention to 6.5~7.5 to adjust pH value.
The above-mentioned seaweed nutritive liquid of the present invention, compound nutritional composition therein is utilized using marine alga comprehensively as raw material, is passed through
Cross the compound processing step of digestion, fermentation, composition, by the macromolecular substances being difficult by marine alga be degraded to micromolecular polysaccharide,
Monose, nitrogen-containing compound, and substantial amounts of beneficial bacterium and a variety of special efficacy metabolites are produced, the composition association for addition of then arranging in pairs or groups again
It is more conducive to play with final nutritional ingredient effect is made；And finally it is prepared containing various compound amino acids, protein, sea
The natural nutrient solution of polysaccharides, maximumlly improve the value of seaweed products.
The nutrition product finally prepared, it can be used after stoste is purified as facial mask, cosmetics etc., it is attached for skin
Put forth effort strong, complete, the comprehensive Amino Acid of infiltration, make the smooth delicacy of skin, reach the effect of beautifying face and moistering lotion；Or it can incite somebody to action
Stoste purifies, and adds different fruits and vegetables, makes the beverage of different mouthfeels, nutritious, be easy to absorb, and can also need do according to human body
Into extraordinary beverage；Or liquid fertilizer is made, it can be provided for crop outside nutrition, also having has improvement to soil, improves quality
Effect.
In the above-mentioned preparation method step of the present invention, step S10 first obtains high-quality marine alga, and technical staff can in implementation
Using the marine alga used as the one or more in sea-tangle, sargassum, bulk kelp, agar, fragrant plant mentioned in ancient texts, seaweed, Enteromorpha or microalgae.Will
The raw material of the select water washing by soaking of 5~15 times of volumes, on the one hand rejects miscellaneous algae and foreign matter, on the other hand can be with desalination.
Also, the Elements in Organic Solvents of some intersolubilities can be added in water during washing, can further adsorbing and removing
The heavy metal of marine alga.Lift the quality of follow-up nutritional ingredient.The process of washing is carried out 2~4 times according to raw material condition.
In order to be further able to that preferably the nutritional ingredient in marine alga is extracted, the progress of step S20 seaweed products is in small, broken bits, in small, broken bits
To the size of 2~5mm particle diameters；Then, addition carries out protopepsia with sodium pyrophosphate to marine alga, preferably used in implementation pH for
7.5~8.0 sodium pyrophosphate buffer solution is carried out, and tentatively alginic cell wall is digested under comparatively gentle environment, is made interior
The preliminary dissolution of inclusion, and itself is easy to reaction to carry out as liquid phase medium.Certainly, if technical staff adds in force
Be sodium pyrophosphate powder, then after addition, the water of 1 ~ 2 times of volume can be supplemented into reaction system, then stirring carry out
Predigestion.The process of predigestion is carried out in 55~60 DEG C of water bath condition, and is stirred continuously 2~3h, is obtained green delicate fragrance and is omited
Micro- sticky predigestion slurries.
Further step S40 carries out further depth digestion to predigestion, the complete mode of action of use, enzymolysis
During used enzyme be 0.1~0.5% Pomacea canaliculata digestive ferment in 0.1~0.2% compound enzyme system of circumscribed 1,4 beta-glucanase.
In addition to the digestion power with relatively good comprehensive cell membrane, avoid by other molecular substances, such as pectin, protein
Deng interference.Meanwhile assisting ultrasonic during enzymolysis, the 25KHz of usual equipment intermittence can be used ultrasonic in implementation
Ripple, time 4s, interval 5s, make the structure of cell membrane more easily broken, the cell membrane of plant can be thoroughly cracked, by marine alga
Cellulose, the main component such as protein separates, and is converted into the available monose of microorganism, oligosaccharides and floating preteins
Matter, be advantageous to improve the recovery rate of inclusion.
Also, technical staff can be carried out step by step in enzymolysis step S40 implementation process：Such as first with circumscribed β-Portugal
Dextranase digests 3h, and enzymolysis process is stirred continuously；Then Pomacea canaliculata digestive ferment enzymolysis 3h is added again；Substep is thin during carrying out
The structure of cell wall is progressively slowly degraded, and content progressively discharges, and is advantageous to keep the microenvironment of enzymolysis and discharged marine alga
The activity of nutrients.
Enzymolysis, digestion centrifuges after completing to enzymolysis liquid, removes the solid phase residue that can not be digested, can be contained
Comprehensive marine alga includes the enzymolysis liquid of nutrients.In enzymolysis liquid except main trehalose, albumen nutritional ingredient in addition to, also have
Substantial amounts of fucoidan, algal polysaccharides (through enzymolysis to Tang oligosaccharide), mannitol, chlorophyll, carrotene, class
Carrotene, vitamin, nicotinic acid, folic acid, pantothenic acid, macroelement, trace element, iodine and marine alga enzyme isoreactivity material.Then step
S50 carries out sterilization to enzymolysis liquid, and process uses is heated to 85~90 DEG C of 10~25min of holding by step S40 enzymolysis liquid.
Further step S60 is fermented the enzymolysis liquid after sterilizing as fermentation substrate inoculation mixed bacteria, the step
The mixed bacteria of middle use is made up of saccharomycete, bifidobacterium breve, lactobacillus plantarum, belongs to anaerobic fermentation；The process of fermentation
With purpose primarily to the macromolecular substances being difficult by marine alga are degraded into micromolecular polysaccharide, compound amino acid, polypeptide,
And further diversity it can increase a variety of special efficacy metabolites；Without using a large amount of nutritional ingredients in enzymolysis liquid as
Substrate conversion into saccharomycete, bifidobacterium breve, lactobacillus plantarum the metabolite such as ethanol lactic acid；Therefore controlled in the step
The ratio of composite bacteria is saccharomycete：Bifidobacterium breve：Lactobacillus plantarum is 1：0.5~0.8：0.5~0.8；And according to
3%~5% inoculum concentration is added in enzymolysis liquid, and the fermentation processes time after inoculation is 0.5~1.5d.
By metabolism in zymotic fluid after fermentation completion, eliminate many unserviceable macromoleculars and fermented
Caused organic acid, biacetyl and low-molecular-weight antibiotic substances etc. are abundant in journey and nutritional ingredient, and be in faintly acid, with comparing
Good antibacterial effect；Greatly improve the application value of seaweed nutritive composition, make product can be used for cosmetics, it is edible or
In fertilizer.
Further step S70 removes thalline and takes supernatant to the zymotic fluid after fermentation completion, centrifugation, is finally sought in addition
Support mannitol, the vitamin E of balance and function auxiliary；Both functional components, in addition to itself is nutriment, add it
Latter aspect contributes to acid-base balance；During as this kind of products application of cosmetics, except itself it is anti-oxidant, repair effect it
Outside, acid-base balance can aid in adjustment nutrient solution in the normal pH scopes of skin, outside product use, moreover it is possible to promote
The effect of reaching balance skin surface p H；And the final growth and breeding for effectively suppressing skin surface harmful bacteria such as Propiobacterium,
So as to reach good beauty, anti-acne effect.On the other hand, have and in seaweed nutritive composition a variety of small molecules organic into
Point, there is relatively good compatibility and absorbability, can be affine compatible with CH, OH group of mannitol, vitamin E, lifting battalion
The collaboration formed point absorbs.
On the basis of above-mentioned seaweed nutritive liquid and preparation method thereof, the present invention further also proposes one kind by above method step
The seaweed nutritive liquid being prepared.Seaweed nutritive liquid prepared by the present invention, using marine alga as raw material comprehensively using therein multiple
Nutritional ingredient is closed, by digesting, fermenting, the processing step that composition is compound, the macromolecular substances being difficult by marine alga are degraded
For micromolecular polysaccharide, monose, nitrogen-containing compound, and substantial amounts of beneficial bacterium and a variety of special efficacy metabolites are produced, then taken again
Composition collaboration with addition makes final nutritional ingredient effect be more conducive to play；And finally it is prepared containing various compound ammonia
Base acid, protein, the natural nutrient solution of algal polysaccharides, there is anticancer, hypotensive, norcholesterol, supplement mineral element, U.S.
Appearance, fat-reducing, anti-aging and the effect for excluding internal heavy metal ion, have embodied huge application potential and high economic valency
Value.
To make the details of the above-mentioned seaweed nutritive liquid and preparation method thereof of the present invention be more conducive to the understanding and implementation of those skilled in the art, with
And the progressive effect of prominent this case seaweed nutritive liquid, the above of this case is lifted below by way of specific embodiment
Example explanation.
Embodiment 1
S10, the clean and tidy sea-tangle 1Kg without go rotten, reject miscellaneous algae and foreign matter is selected, after adding the immersion of 8L water 2 hours, washing of stirring,
Draining；And 3 times are washed repeatedly again in this way to cleaning.
S20, the sea-tangle after step S10 is cleaned are shredded to 2~5mm particulate, are transferred in digestion vessel；
S30, the sodium pyrophosphate buffer solution for the pH8.0 that 100mL concentration is 10% is then added into digestion vessel, then by container
It is placed in 60 DEG C of water bath condition and carries out, and be stirred continuously, obtains the sticky predigestion slurries of green delicate fragrance.
S40, the circumscribed 1,4 beta-glucanases of 1g are added into predigestion liquid, stirring is digested, and digestion vessel is placed in
In ultrasonic device（25KHz intermittence ultrasonic waves, time 4s, interval 5s are set）, digest 3h；Then proceed to 2~3g of addition good fortune
Longevity spiral shell digestive ferment continues to digest 3h；Then the slurries after enzymolysis are centrifuged, discards insoluble precipitation, take supernatant as enzymolysis
Liquid.
S50, enzymolysis liquid is carried out disinfection inactivation, be heated to 85~90 DEG C of 10~25min of holding.
S60, by saccharomycete：Bifidobacterium breve：Lactobacillus plantarum is according to 1：0.7：Bacterial suspension is made in 0.7 ratio,
According to enzyme enzymolysis liquid 3% calculating inoculum concentration and be inoculated with, then carry out fermentation 1d, be then centrifuged for remove thalline, take supernatant for hair
Zymotic fluid.
S70, mannitol, 0.1%~0.4% vitamin E of zymotic fluid weight 2%~5% are added into zymotic fluid, is then adjusted
PH value is to 6.5~7.5, as the seaweed nutritive liquid of the present embodiment 1.
Embodiment 2
S10, high-quality agar, fragrant plant mentioned in ancient texts, seaweed 1Kg are selected, after adding the immersion of 7L water 3 hours, stir washing, draining；And with
This mode washs 4 times to cleaning repeatedly again.
S20, agar, fragrant plant mentioned in ancient texts, the seaweed after step S10 is cleaned are in small, broken bits to 2~5mm in the lump, are transferred to digestion vessel
In；
S30, the sodium pyrophosphate buffer solution for the pH8.0 that 100mL concentration is 10% is then added into digestion vessel, then by container
It is placed in 60 DEG C of water bath condition and carries out, and be stirred continuously, obtains the sticky predigestion slurries of green delicate fragrance.
S40, the circumscribed 1,4 beta-glucanases of 1g are added into predigestion liquid, stirring is digested, and digestion vessel is placed in
In ultrasonic device（25KHz intermittence ultrasonic waves, time 4s, interval 5s are set）, digest 3h；Then proceed to 2~3g of addition good fortune
Longevity spiral shell digestive ferment continues to digest 3h；Then the slurries after enzymolysis are centrifuged, discards insoluble precipitation, take supernatant as enzymolysis
Liquid.
S50, enzymolysis liquid is carried out disinfection inactivation, be heated to 85~90 DEG C of 10~25min of holding.
S60, by saccharomycete：Bifidobacterium breve：Lactobacillus plantarum is according to 1：0.6：Bacterial suspension is made in 0.8 ratio,
According to enzyme enzymolysis liquid 5% calculating inoculum concentration and be inoculated with, then carry out fermentation 1d, be then centrifuged for remove thalline, take supernatant for hair
Zymotic fluid.
S70, mannitol, 0.1%~0.4% vitamin E of zymotic fluid weight 2%~5% are added into zymotic fluid, is then adjusted
PH value is to 6.5~7.5, as the seaweed nutritive liquid of the present embodiment 1.
Nutrient solution prepared by the present invention, due to wherein certainly containing a variety of fucoidans, algal polysaccharides (through enzyme
Solution is to Tang oligosaccharide), mannitol, chlorophyll, carrotene, vitamin, nicotinic acid, folic acid, pantothenic acid, macroelement, micro member
Element, therefore be not required to carry out nutritive validity measure.And emphasis measure specifically includes as the function in terms of beauty and make-up this one kind：
1st, the measure of DPPH radical scavenging activities：2ml seaweed nutritive liquid is taken, adds 0 .2mol/LDPPH ethanol solution 2ml,
The lower room temperature reaction 30min of dark, is then extracted with isometric chloroform, takes supernatant to survey OD in 517nm, compare as deionization
Water adds DPPH solution.
2nd, the measure of hydroxyl radical free radical Scavenging activity：Take 2mmol/L copperas solution 1mL, 6mmol/L hydrogen peroxide
1mL, 6mmol/L salicylic acid 1mL, and seaweed nutritive liquid 1mL is added, 30min is stood, using distilled water as reference, at 510nm
Absorbance is determined, calculates the clearance rate of hydroxy radical.
3rd, the measure of total reducing power：1 mL sample is taken, adds phosphate buffer 1mL and 1% potassium ferricyanide that pH is 6.6
Solution 1mL is mixed, and 20 min are incubated at 50 DEG C, adds 10% solution of trichloroacetic acid 1mL, and vibration takes 1mL mixing after mixing
Liquid, 4mL deionized waters and 0.1% mL of liquor ferri trichloridi 0.4 are added, stand 10 min, be blank with deionized water,
Colorimetric is carried out under 700nm, the value of absorbance is bigger, illustrates that reducing power is stronger.
4th, bacteriostatic test：Material probiotics, enteritis sramana, Fu Shi will he, large intestine O157, single increasing ATCC, will congratulate 301, gold
Staphylococcus aureus cowan1, propionibacterium acnes, LB culture mediums, Oxford cup；
S71, activation probiotics, pathogenic bacteria, is incubated overnight.
S72, pathogenic bacteria cell concentration is adjusted to 108 CFU/mL or so, takes 100 μ L bacterium solutions to be coated on LB flat boards respectively.
S73, after bacterium solution drying, sterile Oxford cup is carefully placed into flat board；Seaweed nutritive is added into Oxford cup
Liquid.
S74,37 DEG C of culture 6-8 h, is observed every 2 ~ 3 h.
S75, measure the antibacterial circle diameter in flat board.
Seaweed nutritive liquid prepared by embodiment 2 is in above-mentioned every test, and in DPPH radical scavenging activities, clearance rate reaches
To 34.26%, illustrate that seaweed nutritive liquid manufactured in the present embodiment is weaker to radical scavenging activity；Hydroxyl radical free radical Scavenging activity
Determination experiment, clearance rate 48.25%, illustrate that seaweed nutritive liquid is stronger to hydroxyl radical free radical Scavenging activity；The survey of total reducing power
Fixed experiment, numerical value 0.25, illustrate that seaweed nutritive liquid has the reducing power of appropriateness（May be also in that subsequently with the addition of vitamin
E improves reproducibility）.The antibacterial circle diameter such as following table of last fungistatic effect test：
From above-mentioned oxygen radical removing and antibacterial effect, suppress for the acne pathogen of skin and oxygen radical is clear
Except with relatively good effect, and supplemented with nutrition, there is very good beauty, anti-acne effect.In the present invention
State under conception, seaweed nutritive liquid prepared by the present invention, there is good nutritional ingredient, be also used as fertilizer additive or multiple
The addition of combined foodstuff is applied, and technical staff can voluntarily implement.
The preferred embodiments of the present invention are these are only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, it is included within the scope of the present invention.
Claims (10)
1. a kind of preparation method of seaweed nutritive liquid, it is characterised in that comprise the following steps：
Marine alga raw material is obtained, and carries out processing in small, broken bits；
The sodium pyrophosphate of seaweed weight 0.1~2% is added into the marine alga after in small, broken bits and carries out predigestion in 55~60 DEG C,
Obtain predigestion slurries；
The predigestion slurries are digested under ultrasound condition with complex enzyme, obtain enzymolysis liquid；The complex enzyme includes accounting for
The Pomacea canaliculata digestive ferment of seaweed weight 0.1~0.5%, 0.1~0.2% circumscribed 1,4 beta-glucanase；
The enzymolysis liquid is heated into 85~90 DEG C keeps 10~25min to be sterilized；
Fermentation process is carried out by composite bacteria is added in the enzymolysis liquid after sterilizing, collects zymotic fluid；The composite bacteria by
Saccharomycete, bifidobacterium breve, lactobacillus plantarum composition；
Mannitol, 0.1%~0.4% vitamin E of zymotic fluid weight 2%~5% are added into the zymotic fluid, and adjusts pH value extremely
6.5~7.5.
2. the preparation method of seaweed nutritive liquid as claimed in claim 1, it is characterised in that saccharomycete in the composite bacteria：
Bifidobacterium breve：Lactobacillus plantarum is 1：0.5~0.8：0.5~0.8.
3. the preparation method of seaweed nutritive liquid as claimed in claim 1 or 2, it is characterised in that by the enzymolysis after sterilizing
Composite bacteria is added in liquid and carries out fermentation process, is collected in zymotic fluid step,
According to 3%~5% inoculum concentration composite bacteria is added into the enzymolysis liquid.
4. the preparation method of the seaweed nutritive liquid as described in right will require 1 or 2, it is characterised in that by the enzyme after sterilizing
Solve and composite bacteria progress fermentation process added in liquid, collect in zymotic fluid step,
0.5~1.5d of time control of the fermentation process.
5. the preparation method of the seaweed nutritive liquid as described in right will require 1 or 2, it is characterised in that by the predigestion slurries
Carried out with complex enzyme under ultrasound condition in enzymolysis step, the process of enzymolysis includes：
Circumscribed 1,4 beta-glucanase enzymolysis is first added into the predigestion slurries, then adds Pomacea canaliculata digestive ferment enzymolysis.
6. the preparation method of seaweed nutritive liquid as claimed in claim 1 or 2, it is characterised in that use the predigestion slurries
Complex enzyme is carried out in enzymolysis step under ultrasound condition,
The ultrasound condition is 25KHz intermittent ultrasonic wave.
7. the preparation method of seaweed nutritive liquid as claimed in claim 1 or 2, it is characterised in that to the marine alga after in small, broken bits
The middle sodium pyrophosphate for adding seaweed weight 0.1~2% is simultaneously carried out in predigestion step in 55~60 DEG C,
The sodium pyrophosphate uses pH to be added for 7.5~8.0 sodium pyrophosphate buffer solution.
8. the preparation method of seaweed nutritive liquid as claimed in claim 1 or 2, it is characterised in that obtain marine alga raw material, and carry out
In processing step in small, broken bits,
The marine alga raw material is in small, broken bits to 2~5mm of particle diameter.
9. the preparation method of seaweed nutritive liquid as claimed in claim 1 or 2, it is characterised in that the marine alga is sea-tangle, horse hair
One or more in algae, bulk kelp, agar, fragrant plant mentioned in ancient texts, seaweed, Enteromorpha or microalgae.
10. a kind of seaweed nutritive liquid, it is characterised in that using the preparation of the seaweed nutritive liquid described in any one of claim 1 to 9
Method prepares.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108158884A (en) * | 2018-02-01 | 2018-06-15 | 百朗德生物化学(海门)有限公司 | With the cosmetic material composition and its application in cosmetics for inhibiting allergy, function of delaying senility |
| CN108300676A (en) * | 2018-02-10 | 2018-07-20 | 厦门昶青生物科技有限公司 | A kind of red seaweed polysaccharide composite bacteria agent and its preparation method and application |
| CN108371215A (en) * | 2018-01-31 | 2018-08-07 | 佛山科学技术学院 | A kind of fermented type microalgae vegetable protein composite beverage and preparation method thereof |
| CN108669483A (en) * | 2018-05-30 | 2018-10-19 | 北京师范大学珠海分校 | A kind of sargassum fermentation product and its liquid fermentation preparation process and application |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102134532A (en) * | 2010-12-05 | 2011-07-27 | 郭静静 | Preparation method of biologically fermented seaweed nutrient liquor |
| CN102488253A (en) * | 2011-12-28 | 2012-06-13 | 青岛明月海洋科技有限公司 | Preparation method of active seaweed extract |
| CN103468751A (en) * | 2013-09-29 | 2013-12-25 | 上海海洋大学 | Method for preparing bioethanol through green tide algae enteromorpha in enzymolysis mode |
| CN103622106A (en) * | 2013-11-21 | 2014-03-12 | 威海家晓食品坊有限公司 | Kelp fermented beverage brewing method |
| CN104341536A (en) * | 2013-08-08 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method for high-efficiency extraction of nutrient substances in seaweed |
| CN104720051A (en) * | 2015-02-07 | 2015-06-24 | 中国海洋大学 | Method for preparing seaweed beverage employing eucheuma extracting solution |
| CN105852115A (en) * | 2016-05-16 | 2016-08-17 | 福建亿达食品有限公司 | Production method for algae enzymes |
-
2017
- 2017-08-14 CN CN201710693003.1A patent/CN107510042A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102134532A (en) * | 2010-12-05 | 2011-07-27 | 郭静静 | Preparation method of biologically fermented seaweed nutrient liquor |
| CN102488253A (en) * | 2011-12-28 | 2012-06-13 | 青岛明月海洋科技有限公司 | Preparation method of active seaweed extract |
| CN104341536A (en) * | 2013-08-08 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method for high-efficiency extraction of nutrient substances in seaweed |
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