CN104341536A - Method for high-efficiency extraction of nutrient substances in seaweed - Google Patents
Method for high-efficiency extraction of nutrient substances in seaweed Download PDFInfo
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- CN104341536A CN104341536A CN201310341395.7A CN201310341395A CN104341536A CN 104341536 A CN104341536 A CN 104341536A CN 201310341395 A CN201310341395 A CN 201310341395A CN 104341536 A CN104341536 A CN 104341536A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a method for high-efficiency extraction of nutrient substances in seaweed, and is characterized in that the method comprises the following steps: (1) raw material pretreatment, (2) water bath immersion, (3) cellulose enzymolysis, (4) proteolysis, (5) brine digestion, (6) dilution and filtration, (7) decoloring, (8) CaCl2 solution treatment, (9) ion exchange, (10) preparation of sodium alginate, (11) preparation of ethanol, and (12) preparation of a seaweed organic fertilizer. A composite enzyme is prepared from protease, pectinase and cellulase by a scientific ratio, and raw material is pretreated, so as to help to improve the extraction rate of sodium alginate; at the same time, fuel ethanol is prepared by using cellulose-rich kelp processing waste; waste liquid and waste residues remaining in the processing process are used for manufacturing the seaweed organic fertilizer through concentration, spray drying and granulation, so as to realize the high-valued comprehensive utilization of kelp and develop marine biological energy.
Description
Technical field
The present invention relates to marine alga and utilize field, be specifically related to a kind of method of high efficiency extraction marine alga Middle nutrition material.
Background technology
Our times is faced with the immense pressure of energy shortage and environmental pollution, and the renewable energy source that exploitation is cleaned has become the important means solving fossil resources and energy dilemma.Bio-ethanol is subject to extensive concern as a kind of clean fuel and gasoline dope.At present, China prepares alcohol fuel and mainly lays particular emphasis on the Lu Yuan raw materials such as stalk, bagasse, wood chip and cassava, but the research being prepared by Living marine resources to bio-ethanol is less, technical study in seaweed biomass energy development backwardness relatively, yet there are no the achievement in research utilizing seaweed processing waste to prepare bio-ethanol.
Ocean area is huge, and be richly stored with marine algae resource, and it is of a great variety, photosynthetic efficiency is high, growth cycle is short, and the biomass total amount produced by photosynthesis every year just reaches 550x10
8ton is a kind of huge renewable resources, is also the potential resources producing biomass energy.Utilize existing economical alga resources advantage production biomass energy, to alleviating the grain of facing mankind, the energy, the large crisis of environment three have huge potentiality.In sea-tangle Industrial processes, produce a large amount of sea-tangle trade wastes, the main component of these trade wastes is Mierocrystalline cellulose, laminarin, N.F,USP MANNITOL etc.; The wherein Mierocrystalline cellulose monose and oligosaccharides that different modes can be utilized to be converted into can be utilized, N.F,USP MANNITOL and most of Sargassum polysaccharides class material can be all ethanol through microbe conversion.The kelp processing waste of rich cellulose, laminarin, N.F,USP MANNITOL is utilized to prepare alcohol fuel, kelp processing waste not only can be made to turn waste into wealth, solve problem of environmental pollution, also can be the raw material supply prepared bio-ethanol and provide sufficient and cheap, promote the technological development of the marine biomass energy.
Sodium alginate, a kind of natural polysaccharide, has the stability needed for pharmaceutical preparation auxiliary material, solvability, viscosity and security.1881, first Englishize scholar E.C.Stanford carried out scientific research to the alginates extract in brown seaweed.He finds that this alignic extract has several very interesting characteristic, and it has concentrated solution, forms the ability of gel and film forming.Based on this, he proposes the application of several suitability for industrialized production.But alginates is until 50 years just carry out large-scale industrial production.Commercially produce and start from nineteen twenty-seven, the present whole world about produces 30000 tons every year, and wherein 30% for foodstuffs industry, and remaining is industrial for other, pharmacy industry and dentistry.
At present, in world wide, sodium alginate extraction method can be divided into four kinds of techniques, is respectively: acid cure acidization, calcium coagulate acidization, calcium coagulates ion exchange method, zymohydrolysis extracting method, and current domestic most producer adopts calcium to coagulate acidization.Wherein the first two kind method is pure chemistry method, because the sodium carbonate of higher concentration digests in leaching process, cause the sodium alginate viscosity produced general lower owing to degrading, productive rate is generally about 20%, and a large amount of pollution of soda acid pharmaceutical chemicals to environment is larger.And enzyme is as a kind of special biological catalyst existed with protein form, be widely used in industries such as beer, fruit juice fruit wine, weaving, feed, leather, Alcohol Production, and features such as special with it, efficient, environmental protection and enjoy green grass or young crops to narrow.Current zymohydrolysis extracting method sodium alginate extraction is ripe not enough, often all just have employed single cellulase to be hydrolyzed, but composition plant cell wall has the compositions such as cellulase, hemicellulase, pectin, xylogen, on-link mode (OLM) between them or inlay, or obvolvent, so be difficult to single enzyme the cell walls thoroughly cracking plant, content can not be made to discharge completely.
How thoroughly to crack the cell walls of plant, content is discharged completely.Avoid again being subject to other people's molecular substance, as the interference of pectin, protein etc., the present invention considers to make prozyme with proteolytic enzyme, polygalacturonase, cellulase by the proportioning of science, pre-treatment is carried out to raw material, and then on the basis of sodium alginate extraction, make full use of marine alga raw material production ethanol and seaweed organic fertilizer.
Summary of the invention
For solving the technical barrier existed in above-mentioned technology, the object of this invention is to provide a kind of method of high efficiency extraction marine alga Middle nutrition material, achieve the extraction yield and viscosity thereof that improve sodium alginate, reduce the consumption of pharmaceutical chemicals, be also that exploitation and the sea-tangle of ethanol cleans, the novel method of high-valued, comprehensive utilization.
For achieving the above object, the invention provides a kind of method of high efficiency extraction marine alga Middle nutrition material, it is characterized in that, described method comprises the steps:
(1) raw materials pretreatment: marine alga raw material is removed silt, dries in 60-70 DEG C or dries, and pulverizes;
(2) water-bath is soaked: add raw material weight 7-8 fresh tap water doubly to above-mentioned seaweed powder, soak 2-2.5h in 50-60 DEG C of water-bath;
(3) fiber enzymolysis: and adjust liquid pH value to 3-6 with HCl, add the cellulase 0.3-0.5% be made up of inscribe β-dextranase 45%, circumscribed β-dextranase 35%, β-glucose lucerne enzyme 20%, enzymolysis 6-10h;
(4) proteolysis: add the proteinase-10 .2-0.3% be made up of neutral protease 50%, papoid 50%, the polygalacturonase 0.2-0.4% be made up of pectin lyase 60%, Rohapect MPE 40%, continues enzymolysis 3-5h, stirs, centrifugation after enzymolysis;
(5) salt solution digestion: to above-mentioned centrifugal after sea-tangle in add the 1-3% Na of 12-15 times of sea-tangle weight
2cO
3solution, digests 2-3h in 50-60 DEG C of water-bath;
(6) dilute filtration: the fresh tap water then adding volume 4-7 times 50-60 DEG C carries out dilution 1-2h, fully after dilution evenly, is filtered by 280-350 object tulle;
(7) decolour: filtrate adds the hydrogen peroxide of 0.2-0.3%, at 60 DEG C of decolouring 1-2h, be then warmed up to 85-90 DEG C and keep 10-25min, filter with 280-350 object tulle;
(8) CaCl
2solution-treated; Filtrate slowly adds the CaCl of volume ratio 1-3% concentration 10%
2solution, leaves standstill 1-1.5h, then filters, with CaCl residual in fresh tap water cleaning calcium alginate fiber
2solution and other impurity;
(9) ion-exchange: calcium alginate adds 12-14 purified water doubly, then by 1.5-2 doubly 10% NaCl solution, carries out ion-exchange, makes calcium alginate fiber be converted into sodium alginate solution completely in 50 DEG C of water-baths;
(10) sodium alginate processed: then use the alcohol precipitation of 95%, obtains sodium alginate, and sodium alginate obtains finished product through dehydration, oven dry, pulverizing;
(11) ethanol processed: the centrifugal filtrate autoclaving in step (4), after cooling, adds activated yeast, and inoculative proportion is 15-20%, 27-35 DEG C and seals and continue to cultivate fermentation in 4-8 days, and ethanol is produced in fermented liquid distillation;
(12) seaweed organic fertilizer processed: contain a large amount of compositions such as protein, nitrogen, phosphorus, potassium, the liquid after ethanol evaporation in remaining liquid after distillation ethanol, after being concentrated to 45-60% concentration, spray is dry, granulation, prepares seaweed organic fertilizer.
The method of above-mentioned a kind of high efficiency extraction marine alga Middle nutrition material, is characterized in that: fermentative production of ethanol in described step (11), after enzymolysis solution sterilizing, accesses activated industrial yeast bacterial classification CICC31447, carries out fermentation culture.
The method of above-mentioned a kind of high efficiency extraction marine alga Middle nutrition material, is characterized in that: described step (11) fermentative production of ethanol, and enzymolysis solution is through the yeast of overactivation at 30 DEG C, and fermentation culture 7 days, the bio-ethanol amount obtained is maximum.
Beneficial effect of the present invention:
The present invention makes prozyme with proteolytic enzyme, polygalacturonase, cellulase by the proportioning of science, avoid being subject to other people's molecular substance, as the interference of pectin, protein etc., pre-treatment is carried out to raw material, thoroughly crack the cell walls of plant, the main components such as the Mierocrystalline cellulose in sea-tangle, protein are separated, and is converted into the available monose of microorganism, oligosaccharides and free protein, be conducive to the extraction yield improving sodium alginate; Utilize the kelp processing waste of rich cellulose to prepare alcohol fuel simultaneously; Remaining waste liquid and waste residue in the course of processing, by concentrating, spraying dry, granulation manufacture seaweed organic fertilizer, achieve the high-valued comprehensive utilization of sea-tangle, develop the marine organisms energy.
Embodiment
embodiment 1:
Marine alga raw material is removed silt, in 60 DEG C of oven dry, accurately takes and dry the 100g sea-tangle after pulverizing, add the fresh tap water of raw material weight 700ml, in 50 DEG C of water-baths, soak 2h; Then use HCl adjust ph to 3, add the cellulase 0.3% be made up of inscribe β-dextranase 45%, circumscribed β-dextranase 35%, β-glucose lucerne enzyme 20%, enzymolysis 6h; Then, add the proteinase-10 .2% be made up of neutral protease 50%, papoid 50%, the polygalacturonase 0.2% be made up of pectin lyase 60%, Rohapect MPE 40%, continue enzymolysis 3h, enzymolysis process constantly stirs, enzymolysis terminates rear centrifugation, obtains pretreated sea-tangle and enzymolysis solution.
Pretreated sea-tangle adds 1% Na of 1200ml
2cO
3solution, digests 2h in 50 DEG C of water-baths, and the fresh tap water then adding 50 DEG C of volume 4000ml carries out dilution 1h, fully after dilution evenly, is filtered by 280 object tulles; Filtrate adds the hydrogen peroxide of 6ml, at 60 DEG C of decolouring 1h, is then warmed up to 85 DEG C and keeps 10min, filter with 280 object tulles; Filtrate slowly adds the CaCl of 60ml concentration 10%
2solution, is converted into calcium alginate and separates out, leave standstill 1h, then filter by sodium alginate wherein, with CaCl residual in fresh tap water cleaning calcium alginate fiber
2solution and other impurity; Calcium alginate adds 2400ml purified water, then 10% NaCl solution of 2000ml is used, ion-exchange is carried out in 50 DEG C of water-baths, calcium alginate fiber is made to be converted into sodium alginate solution completely, then the alcohol precipitation of 95% is used, obtain sodium alginate, sodium alginate obtains finished product 23.3 grams through dehydration, oven dry, pulverizing.
First industrial yeast is placed in nutrient solution and cultivates activation, medium component is 1% yeast extract paste, 2% peptone, 2% glucose, above raw material is dissolved in water, in 121 DEG C of sterilizing 15min, then inoculate in clean operator's console, cultivate 1 day, by pretreated for sea-tangle enzymolysis solution autoclaving, after cooling for 30 DEG C, add activated yeast, inoculative proportion is 10%, 28 DEG C and seals and continue cultivation fermentation in 3 days, and ethanol is produced in fermented liquid distillation, after cultivation terminates, the alcohol concn measured wherein is about 3.2%, after distillation, obtains bio-ethanol.Contain a large amount of compositions such as protein, nitrogen, phosphorus, potassium in remaining liquid after distillation ethanol, the liquid after ethanol evaporation is by the concentrated raw material as producing seaweed organic fertilizer
Bio-ethanol fermented distilled go out after ethanol, it is 50% that remaining fermentation raffinate is concentrated to concentration, and then spray is dry, and pulverize, make seaweed organic fertilizer, the content of the N P and K in fertilizer is about 12%, and the content of total organic matter is 42%.
embodiment 2:
Marine alga raw material is removed silt, in 70 DEG C of oven dry, accurately takes and dry the 100g sea-tangle after pulverizing, add the fresh tap water of raw material weight 800ml, in 60 DEG C of water-baths, soak 2.5h; Then use HCl adjust ph to 6, add the cellulase 0.5% be made up of inscribe β-dextranase 45%, circumscribed β-dextranase 35%, β-glucose lucerne enzyme 20%, enzymolysis 10h; Then, add the proteinase-10 .3% be made up of neutral protease 50%, papoid 50%, the polygalacturonase 0.4% be made up of pectin lyase 60%, Rohapect MPE 40%, continue enzymolysis 5h, enzymolysis process constantly stirs, enzymolysis terminates rear centrifugation, obtains pretreated sea-tangle and enzymolysis solution.
Pretreated sea-tangle adds 3% Na of 1500ml
2cO
3solution, digests 3h in 60 DEG C of water-baths, and the fresh tap water then adding 60 DEG C of volume 7000ml carries out dilution 2h, fully after dilution evenly, is filtered by 350 object tulles; Filtrate adds the hydrogen peroxide of 9ml, at 60 DEG C of decolouring 2h, is then warmed up to 90 DEG C and keeps 25min, filter with 350 object tulles; Filtrate slowly adds the CaCl of 60ml concentration 10%
2solution, is converted into calcium alginate and separates out, leave standstill 1.5h, then filter by sodium alginate wherein, with CaCl residual in fresh tap water cleaning calcium alginate fiber
2solution and other impurity; Calcium alginate adds 2800ml purified water, then 10% NaCl solution of 3500ml is used, ion-exchange is carried out in 50 DEG C of water-baths, calcium alginate fiber is made to be converted into sodium alginate solution completely, then the alcohol precipitation of 95% is used, obtain sodium alginate, sodium alginate obtains finished product 26.3 grams through dehydration, oven dry, pulverizing.
First industrial yeast is placed in nutrient solution and cultivates activation, medium component is 1% yeast extract paste, 2% peptone, 2% glucose, above raw material is dissolved in water, in 121 DEG C of sterilizing 15min, then inoculate in clean operator's console, cultivate 1 day, by pretreated for sea-tangle enzymolysis solution autoclaving, after cooling for 30 DEG C, add activated yeast, inoculative proportion is 10%, 28 DEG C and seals and continue cultivation fermentation in 3 days, and ethanol is produced in fermented liquid distillation, after cultivation terminates, the alcohol concn measured wherein is about 3.2%, after distillation, obtains bio-ethanol.Contain a large amount of compositions such as protein, nitrogen, phosphorus, potassium in remaining liquid after distillation ethanol, the liquid after ethanol evaporation is by the concentrated raw material as producing seaweed organic fertilizer
Bio-ethanol fermented distilled go out after ethanol, it is 50% that remaining fermentation raffinate is concentrated to concentration, and then spray is dry, and pulverize, make seaweed organic fertilizer, the content of the N P and K in fertilizer is about 12%, and the content of total organic matter is 42%.
Claims (3)
1. a method for high efficiency extraction marine alga Middle nutrition material, is characterized in that, described method comprises the steps:
(1) raw materials pretreatment: marine alga raw material is removed silt, dries in 60-70 DEG C or dries, and pulverizes;
(2) water-bath is soaked: add raw material weight 7-8 fresh tap water doubly to above-mentioned seaweed powder, soak 2-2.5h in 50-60 DEG C of water-bath;
(3) fiber enzymolysis: and adjust liquid pH value to 3-6 with HCl, add the cellulase 0.3-0.5% be made up of inscribe β-dextranase 45%, circumscribed β-dextranase 35%, β-glucose lucerne enzyme 20%, enzymolysis 6-10h;
(4) proteolysis: add the proteinase-10 .2-0.3% be made up of neutral protease 50%, papoid 50%, the polygalacturonase 0.2-0.4% be made up of pectin lyase 60%, Rohapect MPE 40%, continues enzymolysis 3-5h, stirs, centrifugation after enzymolysis;
(5) salt solution digestion: to above-mentioned centrifugal after sea-tangle in add the 1-3% Na of 12-15 times of sea-tangle weight
2cO
3solution, digests 2-3h in 50-60 DEG C of water-bath;
(6) dilute filtration: the fresh tap water then adding volume 4-7 times 50-60 DEG C carries out dilution 1-2h, fully after dilution evenly, is filtered by 280-350 object tulle;
(7) decolour: filtrate adds the hydrogen peroxide of 0.2-0.3%, at 60 DEG C of decolouring 1-2h, be then warmed up to 85-90 DEG C and keep 10-25min, filter with 280-350 object tulle;
(8) CaCl
2solution-treated: filtrate slowly adds the CaCl of volume ratio 1-3% concentration 10%
2solution, leaves standstill 1-1.5h, then filters, with CaCl residual in fresh tap water cleaning calcium alginate fiber
2solution and other impurity;
(9) ion-exchange: calcium alginate adds 12-14 purified water doubly, then by 1.5-2 doubly 10% NaCl solution, carries out ion-exchange, makes calcium alginate fiber be converted into sodium alginate solution completely in 50 DEG C of water-baths;
(10) sodium alginate processed: then use the alcohol precipitation of 95%, obtains sodium alginate, and sodium alginate obtains finished product through dehydration, oven dry, pulverizing;
(11) ethanol processed: the centrifugal filtrate autoclaving in step (4), after cooling, adds activated yeast, and inoculative proportion is 15-20%, 27-35 DEG C and seals and continue to cultivate fermentation in 4-8 days, and ethanol is produced in fermented liquid distillation;
(12) seaweed organic fertilizer processed: contain a large amount of compositions such as protein, nitrogen, phosphorus, potassium, the liquid after ethanol evaporation in remaining liquid after distillation ethanol, after being concentrated to 45-60% concentration, spray is dry, granulation, prepares seaweed organic fertilizer.
2. the method for a kind of high efficiency extraction marine alga Middle nutrition material according to claim 1, is characterized in that: fermentative production of ethanol in described step (11), after enzymolysis solution sterilizing, accesses activated industrial yeast bacterial classification CICC31447, carries out fermentation culture.
3. the method for a kind of high efficiency extraction marine alga Middle nutrition material according to claim 1, it is characterized in that: described step (11) fermentative production of ethanol, enzymolysis solution is through the yeast of overactivation at 30 DEG C, and fermentation culture 7 days, the bio-ethanol amount obtained is maximum.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105440154A (en) * | 2015-12-09 | 2016-03-30 | 江苏明月海洋科技有限公司 | Method for extracting alginate in brown algae |
| CN106496351A (en) * | 2016-11-27 | 2017-03-15 | 威海蓝印海洋生物科技有限公司 | A kind of method of nutrient substance in high efficiency extraction Sargassum |
| CN106701310A (en) * | 2016-12-05 | 2017-05-24 | 华南理工大学 | Comprehensive utilization method of green algae biomass resources |
| CN107183067A (en) * | 2017-06-08 | 2017-09-22 | 中国水产科学研究院黄海水产研究所 | A kind of method that overall biologic enzymolysis method prepares seaweed plants growth regulator |
| CN107510042A (en) * | 2017-08-14 | 2017-12-26 | 刘桂连 | Seaweed nutritive liquid and preparation method thereof |
| CN107573124A (en) * | 2017-10-09 | 2018-01-12 | 南昌大学 | A kind of alga fertilizer preparation method using very big kelp as raw material |
| CN107916258A (en) * | 2016-10-11 | 2018-04-17 | 北京工商大学 | For extracting the enzyme preparation of seaweed iodine solution and the method for extraction seaweed iodine solution |
| CN109796541A (en) * | 2018-12-27 | 2019-05-24 | 青岛海之林生物科技开发有限公司 | A kind of meat products specific complex alginates cleanly production technique |
| CN110157754A (en) * | 2019-05-19 | 2019-08-23 | 青岛海之林生物科技开发有限公司 | A kind of production technology of water soluble marine BIOLOGICAL CALCIUM |
| CN110615856A (en) * | 2019-08-14 | 2019-12-27 | 山东劲脉植物细胞信息技术有限公司 | Extraction method of seaweed active ingredients |
| CN110922501A (en) * | 2019-10-24 | 2020-03-27 | 浙江工业大学 | Process for extracting sodium alginate by using ultrasonic-assisted complex enzyme |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105440154A (en) * | 2015-12-09 | 2016-03-30 | 江苏明月海洋科技有限公司 | Method for extracting alginate in brown algae |
| CN107916258A (en) * | 2016-10-11 | 2018-04-17 | 北京工商大学 | For extracting the enzyme preparation of seaweed iodine solution and the method for extraction seaweed iodine solution |
| CN106496351A (en) * | 2016-11-27 | 2017-03-15 | 威海蓝印海洋生物科技有限公司 | A kind of method of nutrient substance in high efficiency extraction Sargassum |
| CN106701310A (en) * | 2016-12-05 | 2017-05-24 | 华南理工大学 | Comprehensive utilization method of green algae biomass resources |
| CN107183067A (en) * | 2017-06-08 | 2017-09-22 | 中国水产科学研究院黄海水产研究所 | A kind of method that overall biologic enzymolysis method prepares seaweed plants growth regulator |
| CN107510042A (en) * | 2017-08-14 | 2017-12-26 | 刘桂连 | Seaweed nutritive liquid and preparation method thereof |
| CN107573124A (en) * | 2017-10-09 | 2018-01-12 | 南昌大学 | A kind of alga fertilizer preparation method using very big kelp as raw material |
| CN109796541A (en) * | 2018-12-27 | 2019-05-24 | 青岛海之林生物科技开发有限公司 | A kind of meat products specific complex alginates cleanly production technique |
| CN110157754A (en) * | 2019-05-19 | 2019-08-23 | 青岛海之林生物科技开发有限公司 | A kind of production technology of water soluble marine BIOLOGICAL CALCIUM |
| CN110157754B (en) * | 2019-05-19 | 2023-01-20 | 青岛海之林生物科技开发有限公司 | Production process of water-soluble marine organism calcium |
| CN110615856A (en) * | 2019-08-14 | 2019-12-27 | 山东劲脉植物细胞信息技术有限公司 | Extraction method of seaweed active ingredients |
| CN110922501A (en) * | 2019-10-24 | 2020-03-27 | 浙江工业大学 | Process for extracting sodium alginate by using ultrasonic-assisted complex enzyme |
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Application publication date: 20150211 |