CN106496351A - A kind of method of nutrient substance in high efficiency extraction Sargassum - Google Patents
A kind of method of nutrient substance in high efficiency extraction Sargassum Download PDFInfo
- Publication number
- CN106496351A CN106496351A CN201611058261.4A CN201611058261A CN106496351A CN 106496351 A CN106496351 A CN 106496351A CN 201611058261 A CN201611058261 A CN 201611058261A CN 106496351 A CN106496351 A CN 106496351A
- Authority
- CN
- China
- Prior art keywords
- water
- ethanol
- sodium alginate
- solution
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0084—Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Sustainable Development (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses in a kind of high efficiency extraction Sargassum nutrient substance method, it is characterised in that described method comprises the steps：(1) pretreatment of raw material (2) water-bath immersion (3) fiber enzymolysis (4) proteolysiss (5) saline digestion (6) dilute filtration (7) decolouring (8) CaCl2Solution processes (9) ion exchange (10) sodium alginate (11) processed ethanol (12) processed seaweed organic fertilizer processed.The present invention makes compound enzyme with protease, pectase, cellulase by the proportioning of science, carries out pretreatment to raw material, is conducive to the extraction ratio for improving sodium alginate；Alcohol fuel is prepared simultaneously using the kelp processing waste of rich cellulose；Remaining waste liquid and waste residue in the course of processing, manufacture seaweed organic fertilizer by concentration, spray drying, pelletize, it is achieved that the high-valued comprehensive utilization of Thallus Laminariae (Thallus Eckloniae), develop the marine organisms energy.
Description
Technical field
The present invention relates to Sargassum utilizes field, and in particular to a kind of method of nutrient substance in high efficiency extraction Sargassum.
Background technology
Our times is faced with the immense pressure of energy shortage and environmental pollution, develop the regenerative resource of cleaning into
For solving the important means of fossil resources and energy crisis.Bio-ethanol is subject to as a kind of clean fuel and gasoline additive
Extensive concern.At present, China prepares alcohol fuel and primarily focuses on the Lu Yuan raw materials such as straw, bagasse, wood flour and Maninot esculenta crantz., but right
The research that living marine resources prepare bio-ethanol is less, and the technical research in terms of seaweed biomass energy development falls relatively
Afterwards, the achievement in research for preparing bio-ethanol using seaweed processing waste yet there are no.
Ocean area is huge, and be richly stored with marine algae resource, and its species is various, photosynthetic efficiency is high, growth cycle
Short, the biomass total amount that can be produced by photosynthesis every year, just up to 550x108 tons, is a kind of huge Renewable resource,
It is the potential resource for producing biomass energy.Biomass energy is produced using existing economical alga resources advantage, to alleviating people
Grain that class faces, the energy, three big crisis of environment have huge potentiality.In Thallus Laminariae (Thallus Eckloniae) industrial processes, substantial amounts of sea is produced
Band trade waste, the main component of these trade wastes is cellulose, laminarin, Mannitol etc.；Wherein cellulose can
By using being converted into the monosaccharide and oligosaccharide that can be utilized in the way of different, Mannitol and most of Sargassum polysaccharides class material can be with
Fermented it is converted into ethanol.Alcohol fuel is prepared using the kelp processing waste of rich cellulose, laminarin, Mannitol,
Can not only make kelp processing waste turn waste into wealth, solve problem of environmental pollution, can also for prepare bio-ethanol provides sufficient with
Cheap raw material supply, promotes the technological development of the marine biomass energy.
Sodium alginate, a kind of natural polysaccharide, with the stability needed for pharmaceutical preparation adjuvant, dissolubility, viscosity and safety
Property.1881, Britain chemist E.C.Stanford first to brown seaweed in alginate extract carry out scientific research.
He has found that the alignic extract has several very interesting characteristics, and it has concentrate solution, forms the energy of gel and film forming
Power.This is based on, he proposes the application of several industrialized production.But, alginate was just carried out afterwards until 50 years on a large scale
Industrialized production.Commercially produce and start from nineteen twenty-seven, the present whole world about produces 30000 tons every year, wherein 30% is used for food work
Industry, remaining for other industry, pharmacy industry and dentistry.
At present, in world wide, sodium alginate extraction method can be divided into four kinds of techniques, respectively：Acid cure acidization, calcium coagulate
Acidization, calcium coagulate ion exchange, zymohydrolysis extracting method, and domestic overwhelming majority producer is using the solidifying acidization of calcium at present.Wherein the first two
The method of kind is pure chemistry method, and the sodium carbonate due to higher concentration in extraction process digests, and causes the sodium alginate that produces
Viscosity is generally relatively low due to degrading, yield typically 20% or so, and substantial amounts of soda acid chemical drugss to the pollution of environment compared with
Greatly.And enzyme is used as a kind of special biocatalyzer existed with protein form, medicated beer, fruit juice fruit wine, weaving, feedstuff,
The industries such as leather, Alcohol Production are widely used, and so which is special, efficiently, environmental protection the features such as and enjoy green grass or young crops to narrow.
Zymohydrolysis extracting method sodium alginate extraction is ripe not enough at present, is all often to employ single cellulase to be hydrolyzed,
But constitute plant cell wall has the compositions such as cellulase, hemicellulase, pectin, lignin, the on-link mode (OLM) between them
Or inlay, or obvolvent, so being difficult the cell wall for thoroughly cracking plant with single enzyme, it is impossible to content is released completely
Put.
The cell wall of plant how is thoroughly cracked, content is discharged completely.Avoided by other people's molecular substances again,
The interference of such as pectin, protein etc., present invention consideration are made compound with protease, pectase, cellulase by the proportioning of science
Enzyme, carries out pretreatment to raw material, then again on the basis of sodium alginate extraction, makes full use of Sargassum raw material production ethanol and Sargassum
Fertilizer.
Content of the invention
For solving technical barrier present in above-mentioned technology, it is an object of the invention to provide seeking in a kind of high efficiency extraction Sargassum
The method of foster material, it is achieved that improve extraction ratio and its viscosity of sodium alginate, the consumption of reduction chemical drugss, and ethanol
Exploitation and Thallus Laminariae (Thallus Eckloniae) clean, the high-valued, new method of comprehensive utilization.
For reaching above-mentioned purpose, the invention provides in a kind of high efficiency extraction Sargassum nutrient substance method, its feature exists
In described method comprises the steps：
（1）Pretreatment of raw material：Sargassum raw material is removed silt, is dried in 60-70 DEG C or is dried, crush；
（2）Water-bath is soaked：To the fresh tap water that above-mentioned Sargassum powder adds 7-8 times of raw material weight, 2- in 50-60 DEG C of water-bath, is soaked
2.5h；
（3）Fiber is digested：And adjust liquid pH value to 3-6 with HCl, add and gathered by inscribe β-glucanase 45%, circumscribed β-Portugal
The cellulase 0.3-0.5% of carbohydrase 35%, β-composition of glucose lucerne enzyme 20%, digests 6-10h；
（4）Proteolysiss：The proteinase-10 .2-0.3% being made up of neutral protease 50%, papain 50% is added, by fruit
Glue lyases 60%, the pectase 0.2-0.4% of the composition of pectinesterase 40%, continue enzymolysis 3-5h, stirring, centrifugation point after enzymolysis
From；
（5）Saline digests：The 1-3% Na2CO3 solution of 12-15 times of Thallus Laminariae (Thallus Eckloniae) weight is added in the Thallus Laminariae (Thallus Eckloniae) after above-mentioned centrifugation,
2-3h is digested in 50-60 DEG C of water-bath；
（6）Dilute filtration：The fresh tap water for being subsequently adding 50-60 DEG C of volume 4-7 times is diluted 1-2h, and fully dilution is uniform
Afterwards, filtered by the tulle of 280-350 mesh；
（7）Decolourize：Filtrate adds the hydrogen peroxide of 0.2-0.3%, in 60 DEG C of decolouring 1-2h, is then warmed up to 85-90 DEG C of holding 10-
25min, is filtered with the tulle of 280-350 mesh；
（8）The process of CaCl2 solution；Filtrate is slowly added to the CaCl2 solution of volume ratio 1-3% concentration 10%, stands 1-1.5h, then
Filter, the CaCl2 solution and other impurity remained in calcium alginate fiber is cleaned with fresh drinking water；
（9）Ion exchange：Calcium alginate adds 12-14 times of purified water, then with 1.5-2 times of 10% NaCl solution, at 50 DEG C
Ion exchange is carried out in water-bath, makes calcium alginate fiber be fully converted to sodium alginate solution；
（10）Sodium alginate processed：Then with 95% alcohol precipitation, obtain sodium alginate, sodium alginate through dehydration, dry, crush
Obtain finished product；
（11）Ethanol processed：Step（4）In centrifugal filtrate autoclaving, after cooling, add activated yeast, inoculation ratio
Example is 15-20%, and 27-35 DEG C seals and continue culture and ferment for 4-8 days, fermentation liquid distillation production ethanol；
（12）Seaweed organic fertilizer processed：Steam containing compositions such as substantial amounts of protein, nitrogen, phosphorus, potassium in remaining liquid after distillation ethanol
The liquid after ethanol is sent out, after the concentrated concentration to 45-60%, spray drying, pelletize prepare seaweed organic fertilizer.
The method of nutrient substance in a kind of above-mentioned high efficiency extraction Sargassum, it is characterised in that：The step（11）Middle fermentation
Production ethanol, after enzymolysis solution sterilizing, accesses activated industrial yeast strain CICC31447, carries out fermentation culture.
The method of nutrient substance in a kind of above-mentioned high efficiency extraction Sargassum, it is characterised in that：The step（11）Fermentation life
Producing and ethanol, enzymolysis solution through overactivation yeast at 30 DEG C, fermentation culture 7 days, the bio-ethanol amount that obtained are maximum.
Beneficial effects of the present invention：
The present invention makes compound enzyme with protease, pectase, cellulase by the proportioning of science, it is to avoid by other people's molecule things
The interference of matter, such as pectin, protein etc., carries out pretreatment to raw material, thoroughly cracks the cell wall of plant, by the fiber in Thallus Laminariae (Thallus Eckloniae)
The main components such as element, protein are separated, and are converted into the available monosaccharide of microorganism, oligosaccharide and free protein, favorably
In the extraction ratio for improving sodium alginate；Alcohol fuel is prepared simultaneously using the kelp processing waste of rich cellulose；Processed
Remaining waste liquid and waste residue in journey, manufacture seaweed organic fertilizer by concentration, spray drying, pelletize, it is achieved that the high-valued synthesis of Thallus Laminariae (Thallus Eckloniae)
Utilize, develop the marine organisms energy.
Specific embodiment
Embodiment 1：
Sargassum raw material is removed silt, is dried in 60 DEG C, accurately weigh the 100g Thallus Laminariae (Thallus Eckloniae)s after dries pulverizing, add raw material weight
The fresh tap water of 700ml, soaks 2h in 50 DEG C of water-baths；Then pH value is adjusted to 3 with HCl, add by inscribe β-glucosan
The cellulase 0.3% of enzyme 45%, circumscribed β-glucanase 35%, β-composition of glucose lucerne enzyme 20%, digests 6h；Then, plus
Enter the proteinase-10 .2% being made up of neutral protease 50%, papain 50%, by pectin lyase 60%, pectinesterase
The pectase 0.2% of 40% composition, continues enzymolysis 3h, and enzymolysis process is stirred continuously, and enzymolysis terminates rear centrifugation, obtains pre- place
Thallus Laminariae (Thallus Eckloniae) and enzymolysis solution after reason.
Pretreated Thallus Laminariae (Thallus Eckloniae) adds 1% Na of 1200ml2CO3Solution, in 50 DEG C of water-baths digests 2h, is subsequently adding body
The fresh tap water of 50 DEG C of product 4000ml is diluted 1h, fully after dilution uniformly, is filtered by the tulle of 280 mesh；Filtrate
The hydrogen peroxide of 6ml is added, in 60 DEG C of decolouring 1h, 85 DEG C of holding 10min is then warmed up to, is filtered with the tulle of 280 mesh；Filtrate is delayed
Sodium alginate therein is converted into calcium alginate precipitation, stands 1h, then mistake by the slow CaCl2 solution for adding 60ml concentration 10%
Filter, cleans the CaCl2 solution and other impurity remained in calcium alginate fiber with fresh drinking water；Calcium alginate is added
2400ml purified water, then with 10% NaCl solution of 2000ml, carries out ion exchange in 50 DEG C of water-baths, makes calcium alginate fine
Dimension be fully converted to sodium alginate solution, then with 95% alcohol precipitation, obtain sodium alginate, sodium alginate through dehydration, baking
Dry, crushing obtains 23.3 grams of finished product.
Industrial yeast is placed in culture activation in culture fluid first, medium component is 1% yeast extract, 2% peptone, 2% Fructus Vitis viniferae
Sugar, above raw material are dissolved in water, and in 121 DEG C of 15min that sterilize, are then inoculated with clean operating board, and 30 DEG C are cultivated 1 day, by sea
With pretreated enzymolysis solution autoclaving, after cooling, activated yeast is added, inoculative proportion is 10%, 28 DEG C of sealings
And continue to cultivate fermentation in 3 days, fermentation liquid distillation production ethanol, after culture terminates, determines concentration of alcohol therein and is about 3.2%, steam
After evaporating, bio-ethanol is obtained.Containing the compositions such as substantial amounts of protein, nitrogen, phosphorus, potassium, evaporation in remaining liquid after distillation ethanol
Liquid after ethanol is by concentration as the raw material for producing seaweed organic fertilizer
Bio-ethanol fermented distilled go out ethanol after, remaining fermentation residual liquid is concentrated to concentration for 50%, and then spray drying, crushes, make
Seaweed organic fertilizer, the content of the N P and K in fertilizer are about 12%, and the content of total organic matter is 42%.
Embodiment 2：
Sargassum raw material is removed silt, is dried in 70 DEG C, accurately weigh the 100g Thallus Laminariae (Thallus Eckloniae)s after dries pulverizing, add raw material weight
The fresh tap water of 800ml, soaks 2.5h in 60 DEG C of water-baths；Then pH value is adjusted to 6 with HCl, add and gathered by inscribe β-Portugal
The cellulase 0.5% of carbohydrase 45%, circumscribed β-glucanase 35%, β-composition of glucose lucerne enzyme 20%, digests 10h；So
Afterwards, the proteinase-10 .3% being made up of neutral protease 50%, papain 50% is added, by pectin lyase 60%, pectin
The pectase 0.4% of the composition of esterase 40%, continues enzymolysis 5h, and enzymolysis process is stirred continuously, and enzymolysis terminates rear centrifugation, obtains
Pretreated Thallus Laminariae (Thallus Eckloniae) and enzymolysis solution.
Pretreated Thallus Laminariae (Thallus Eckloniae) adds the 3% Na2CO3 solution of 1500ml, digests 3h, be subsequently adding body in 60 DEG C of water-baths
The fresh tap water of 60 DEG C of product 7000ml is diluted 2h, fully after dilution uniformly, is filtered by the tulle of 350 mesh；Filtrate
The hydrogen peroxide of 9ml is added, in 60 DEG C of decolouring 2h, 90 DEG C of holding 25min is then warmed up to, is filtered with the tulle of 350 mesh；Filtrate is delayed
The slow CaCl for adding 60ml concentration 10%2Sodium alginate therein is converted into calcium alginate precipitation, stands 1.5h, then by solution
Filter, the CaCl remained in calcium alginate fiber is cleaned with fresh drinking water2Solution and other impurity；Calcium alginate is added
2800ml purified water, then with 10% NaCl solution of 3500ml, carries out ion exchange in 50 DEG C of water-baths, makes calcium alginate fine
Dimension be fully converted to sodium alginate solution, then with 95% alcohol precipitation, obtain sodium alginate, sodium alginate through dehydration, baking
Dry, crushing obtains 26.3 grams of finished product.
Industrial yeast is placed in culture activation in culture fluid first, medium component is 1% yeast extract, 2% peptone, 2% Fructus Vitis viniferae
Sugar, above raw material are dissolved in water, and in 121 DEG C of 15min that sterilize, are then inoculated with clean operating board, and 30 DEG C are cultivated 1 day, by sea
With pretreated enzymolysis solution autoclaving, after cooling, activated yeast is added, inoculative proportion is 10%, 28 DEG C of sealings
And continue to cultivate fermentation in 3 days, fermentation liquid distillation production ethanol, after culture terminates, determines concentration of alcohol therein and is about 3.2%, steam
After evaporating, bio-ethanol is obtained.Containing the compositions such as substantial amounts of protein, nitrogen, phosphorus, potassium, evaporation in remaining liquid after distillation ethanol
Liquid after ethanol is by concentration as the raw material for producing seaweed organic fertilizer
Bio-ethanol fermented distilled go out ethanol after, remaining fermentation residual liquid is concentrated to concentration for 50%, and then spray drying, crushes, make
Seaweed organic fertilizer, the content of the N P and K in fertilizer are about 12%, and the content of total organic matter is 42%.
Claims (1)
1. in a kind of high efficiency extraction Sargassum nutrient substance method, it is characterised in that described method comprises the steps：
（1）Pretreatment of raw material：Sargassum raw material is removed silt, is dried in 60-70 DEG C or is dried, crush；
（2）Water-bath is soaked：To the fresh tap water that above-mentioned Sargassum powder adds 7-8 times of raw material weight, 2- in 50-60 DEG C of water-bath, is soaked
2.5h；
（3）Fiber is digested：And adjust liquid pH value to 3-6 with HCl, add and gathered by inscribe β-glucanase 45%, circumscribed β-Portugal
The cellulase 0.3-0.5% of carbohydrase 35%, β-composition of glucose lucerne enzyme 20%, digests 6-10h；
（4）Proteolysiss：The proteinase-10 .2-0.3% being made up of neutral protease 50%, papain 50% is added, by fruit
Glue lyases 60%, the pectase 0.2-0.4% of the composition of pectinesterase 40%, continue enzymolysis 3-5h, stirring, centrifugation point after enzymolysis
From；
（5）Saline digests：The 1-3% Na2CO3 solution of 12-15 times of Thallus Laminariae (Thallus Eckloniae) weight is added in the Thallus Laminariae (Thallus Eckloniae) after above-mentioned centrifugation,
2-3h is digested in 50-60 DEG C of water-bath；
（6）Dilute filtration：The fresh tap water for being subsequently adding 50-60 DEG C of volume 4-7 times is diluted 1-2h, and fully dilution is uniform
Afterwards, filtered by the tulle of 280-350 mesh；
（7）Decolourize：Filtrate adds the hydrogen peroxide of 0.2-0.3%, in 60 DEG C of decolouring 1-2h, is then warmed up to 85-90 DEG C of holding 10-
25min, is filtered with the tulle of 280-350 mesh；
（8）The process of CaCl2 solution：Filtrate is slowly added to the CaCl of volume ratio 1-3% concentration 10%2Solution, stands 1-1.5h, then
Filter, the CaCl remained in calcium alginate fiber is cleaned with fresh drinking water2Solution and other impurity；
（9）Ion exchange：Calcium alginate adds 12-14 times of purified water, then with 1.5-2 times of 10% NaCl solution, at 50 DEG C
Ion exchange is carried out in water-bath, makes calcium alginate fiber be fully converted to sodium alginate solution；
（10）Sodium alginate processed：Then with 95% alcohol precipitation, obtain sodium alginate, sodium alginate through dehydration, dry, crush
Obtain finished product；
（11）Ethanol processed：Step（4）In centrifugal filtrate autoclaving, after cooling, add activated yeast, inoculation ratio
Example is 15-20%, and 27-35 DEG C seals and continue culture and ferment for 4-8 days, fermentation liquid distillation production ethanol；
（12）Seaweed organic fertilizer processed：Steam containing compositions such as substantial amounts of protein, nitrogen, phosphorus, potassium in remaining liquid after distillation ethanol
The liquid after ethanol is sent out, after the concentrated concentration to 45-60%, spray drying, pelletize prepare seaweed organic fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611058261.4A CN106496351A (en) | 2016-11-27 | 2016-11-27 | A kind of method of nutrient substance in high efficiency extraction Sargassum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611058261.4A CN106496351A (en) | 2016-11-27 | 2016-11-27 | A kind of method of nutrient substance in high efficiency extraction Sargassum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106496351A true CN106496351A (en) | 2017-03-15 |
Family
ID=58328082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611058261.4A Pending CN106496351A (en) | 2016-11-27 | 2016-11-27 | A kind of method of nutrient substance in high efficiency extraction Sargassum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106496351A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107573170A (en) * | 2017-10-09 | 2018-01-12 | 山东沃科奇实业有限公司 | Seaweed bio-organic fertilizer prepared by parallel enzymolysis process and preparation method thereof |
| CN109020666A (en) * | 2018-07-18 | 2018-12-18 | 威海市世代海洋生物科技股份有限公司 | Kelp digests liquid fertilizer and preparation method thereof, application method |
| CN109796541A (en) * | 2018-12-27 | 2019-05-24 | 青岛海之林生物科技开发有限公司 | A kind of meat products specific complex alginates cleanly production technique |
| CN110922501A (en) * | 2019-10-24 | 2020-03-27 | 浙江工业大学 | Process for extracting sodium alginate by using ultrasonic-assisted complex enzyme |
| CN113636892A (en) * | 2021-09-23 | 2021-11-12 | 山东爱果者生物技术有限公司 | Preparation method of seaweed extract and seaweed fertilizer |
| CN113788722A (en) * | 2021-09-23 | 2021-12-14 | 山东爱果者生物技术有限公司 | Biological stimulator and preparation method thereof |
| CN115093271A (en) * | 2022-06-23 | 2022-09-23 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sugarcane sugar increasing agent and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104341536A (en) * | 2013-08-08 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method for high-efficiency extraction of nutrient substances in seaweed |
-
2016
- 2016-11-27 CN CN201611058261.4A patent/CN106496351A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104341536A (en) * | 2013-08-08 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method for high-efficiency extraction of nutrient substances in seaweed |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107573170A (en) * | 2017-10-09 | 2018-01-12 | 山东沃科奇实业有限公司 | Seaweed bio-organic fertilizer prepared by parallel enzymolysis process and preparation method thereof |
| CN109020666A (en) * | 2018-07-18 | 2018-12-18 | 威海市世代海洋生物科技股份有限公司 | Kelp digests liquid fertilizer and preparation method thereof, application method |
| CN109796541A (en) * | 2018-12-27 | 2019-05-24 | 青岛海之林生物科技开发有限公司 | A kind of meat products specific complex alginates cleanly production technique |
| CN110922501A (en) * | 2019-10-24 | 2020-03-27 | 浙江工业大学 | Process for extracting sodium alginate by using ultrasonic-assisted complex enzyme |
| CN113636892A (en) * | 2021-09-23 | 2021-11-12 | 山东爱果者生物技术有限公司 | Preparation method of seaweed extract and seaweed fertilizer |
| CN113788722A (en) * | 2021-09-23 | 2021-12-14 | 山东爱果者生物技术有限公司 | Biological stimulator and preparation method thereof |
| CN115093271A (en) * | 2022-06-23 | 2022-09-23 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sugarcane sugar increasing agent and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104945535B (en) | Method for production of sodium alginate and co-production of ethanol and seaweed organic fertilizer | |
| CN106496351A (en) | A kind of method of nutrient substance in high efficiency extraction Sargassum | |
| CN104341536A (en) | Method for high-efficiency extraction of nutrient substances in seaweed | |
| CN101928733B (en) | Method and device for producing biological butanol with straw-like materials or agricultural and forestry wastes | |
| CN104341534B (en) | A kind of method that sodium alginate and organic fertilizer are produced with sargassum | |
| CN101358214B (en) | Method for producing furfural coupled cogeneration of propanone and butanol using stalk | |
| CN104341535B (en) | A kind of high-valued extracting method of Enteromorpha | |
| CN101358218A (en) | Method for producing xylose coupled cogeneration of propanone, butanol and ethanol using stalk | |
| CN101608192B (en) | Method for producing succinic acid employing corn cob | |
| CN101423855B (en) | Method for preparing polysaccharide by using lucidum strain fermented laminaria leftover | |
| CN102363795A (en) | Method for co-production of lactic acid and alcohol by lignocellulose | |
| CN102051395A (en) | Method for preparing bacterial cellulose from corn stalks | |
| Chen et al. | Prospects for bioethanol production from macroalgae | |
| Ravanal et al. | Production of bioethanol from brown algae | |
| Hessami et al. | Bioethanol a by-product of agar and carrageenan production industry from the tropical red seaweeds, Gracilaria manilaensis and Kappaphycus alvarezii | |
| CN104341537A (en) | Method for arsenic removal and extraction of nutrient substances in sargassum fusiforme | |
| CN108300747B (en) | Method for improving saccharified efficiency of silvergrass enzymolysis by utilizing sodium chlorite pretreatment | |
| CN101845349B (en) | Application of constructed wetland plant biomass resource | |
| CN103352057A (en) | Method for preparing fuel ethanol by taking bamboo shoot processing residues as raw materials | |
| CN102511650B (en) | Method for preparing protein feed by using Jerusalem artichoke residues | |
| CN104341233A (en) | Method for high-efficiency extraction of nutrient substances in undaria pinnatifida sporophyll | |
| CN107502636B (en) | Method for pretreating hybrid pennisetum alopecuroides at low temperature by using ammonia water | |
| RU2421521C2 (en) | Method of producing ethanol from sea weed | |
| CN105039417A (en) | Method for preparing microbial flocculant through enteromorpha hydrolysate fermentation | |
| Sarwar et al. | Alkaline pre-treatment of wheat straw for production of ethanol using saccharomyces cerevisiae ss-4 immobilized in nanoparticles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170315 |