CN101358218A - Method for producing xylose coupled cogeneration of propanone, butanol and ethanol using stalk - Google Patents
Method for producing xylose coupled cogeneration of propanone, butanol and ethanol using stalk Download PDFInfo
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- CN101358218A CN101358218A CNA2008101414189A CN200810141418A CN101358218A CN 101358218 A CN101358218 A CN 101358218A CN A2008101414189 A CNA2008101414189 A CN A2008101414189A CN 200810141418 A CN200810141418 A CN 200810141418A CN 101358218 A CN101358218 A CN 101358218A
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- xylose
- butanols
- propanone
- cogeneration
- stalks
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 title claims abstract description 103
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 title claims abstract description 70
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 title claims abstract description 43
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 title claims abstract description 35
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 title claims abstract description 35
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 14
- 230000007062 hydrolysis Effects 0.000 claims abstract description 36
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 36
- 239000002253 acid Substances 0.000 claims abstract description 28
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 24
- 239000010902 straw Substances 0.000 claims abstract description 20
- 239000012530 fluid Substances 0.000 claims abstract description 14
- 229960003487 xylose Drugs 0.000 claims description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 230000001476 alcoholic effect Effects 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 19
- 240000008042 Zea mays Species 0.000 claims description 16
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 16
- 108010059892 Cellulase Proteins 0.000 claims description 15
- 229940106157 cellulase Drugs 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 11
- 235000005822 corn Nutrition 0.000 claims description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 claims description 8
- 241000193403 Clostridium Species 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 238000010411 cooking Methods 0.000 claims description 6
- 238000002425 crystallisation Methods 0.000 claims description 6
- 230000008025 crystallization Effects 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 6
- 230000008020 evaporation Effects 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 238000005342 ion exchange Methods 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000001117 sulphuric acid Substances 0.000 claims description 6
- 235000011149 sulphuric acid Nutrition 0.000 claims description 6
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 5
- 235000009973 maize Nutrition 0.000 claims description 5
- 241000609240 Ambelania acida Species 0.000 claims description 4
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- 241000193401 Clostridium acetobutylicum Species 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- 240000006394 Sorghum bicolor Species 0.000 claims description 4
- 235000015505 Sorghum bicolor subsp. bicolor Nutrition 0.000 claims description 4
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 4
- 235000021307 Triticum Nutrition 0.000 claims description 4
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- 239000010905 bagasse Substances 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 4
- 241000726221 Gemma Species 0.000 claims description 3
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical group [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 3
- 239000000920 calcium hydroxide Substances 0.000 claims description 3
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 3
- 235000012204 lemonade/lime carbonate Nutrition 0.000 claims description 3
- 235000017550 sodium carbonate Nutrition 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 2
- 244000105624 Arachis hypogaea Species 0.000 claims description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 2
- 235000018262 Arachis monticola Nutrition 0.000 claims description 2
- 244000020551 Helianthus annuus Species 0.000 claims description 2
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 2
- 235000019779 Rapeseed Meal Nutrition 0.000 claims description 2
- 235000019270 ammonium chloride Nutrition 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 235000012343 cottonseed oil Nutrition 0.000 claims description 2
- 235000020232 peanut Nutrition 0.000 claims description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 2
- 235000015320 potassium carbonate Nutrition 0.000 claims description 2
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 2
- 239000004456 rapeseed meal Substances 0.000 claims description 2
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 2
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 8
- 239000001913 cellulose Substances 0.000 abstract description 7
- 229920002678 cellulose Polymers 0.000 abstract description 7
- 239000002699 waste material Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 2
- 229920002488 Hemicellulose Polymers 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000005903 acid hydrolysis reaction Methods 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 241000209140 Triticum Species 0.000 description 3
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 3
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 3
- 239000000811 xylitol Substances 0.000 description 3
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 3
- 229960002675 xylitol Drugs 0.000 description 3
- 235000010447 xylitol Nutrition 0.000 description 3
- 239000000446 fuel Substances 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 239000003973 paint Substances 0.000 description 2
- 150000002972 pentoses Chemical class 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000001308 synthesis method Methods 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- -1 cinefilm Substances 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- ACGUYXCXAPNIKK-UHFFFAOYSA-N hexachlorophene Chemical compound OC1=C(Cl)C=C(Cl)C(Cl)=C1CC1=C(O)C(Cl)=CC(Cl)=C1Cl ACGUYXCXAPNIKK-UHFFFAOYSA-N 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000005504 petroleum refining Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a method for producing xylose co-produced with acetone, butanol and ethanol by utilizing straws. The straws are hydrated by acid to be filtered to be divided into xylose hydrolysis fluid and xylose residue; the xylose residue is zymohydrolyzed, fermented and rectified to prepare the acetone, the butanol and the ethanol; the xylose hydrolysis fluid is used for preparing the xylose. The method of producing xylose co-produced with acetone, butanol and ethanol by utilizing straws of the invention realizes the maximization of the cellulose straw substances, not only the straw resources are fully utilized to realize changing waste into valuable and the added value of products is improved to realize the economic value of the straws, but also the new raw material source and methods for the production of the acetone and the ethanol are developed and more economic benefits and social benefits are brought to the enterprises and the society.
Description
Technical field
The invention belongs to technical field of biochemical industry, relate to a kind of producing xylose with stalks cogeneration of propanone, butanols and alcoholic acid method utilized.
Background technology
Crop stalk is the photosynthetic product of sunlight, is large renewable resources, and only China just gathers in the crops about 800,000,000 tons every year, and except that sub-fraction was used as feed, all the other major parts had all been wasted.Along with the progress of technology, developed the technology of utilizing producing xylose with stalks, Xylitol, for a new way has been opened up in the utilization again of stalk.But in the technology of utilizing producing xylose with stalks, Xylitol, also exist some shortcomings, liquid part after the acid hydrolysis of stalk process, the filtration is used to produce wood sugar, but the xylose residue after filtering is not then utilized fully, there is part to be used to prepare ethanol, but do not realize the maximization of straw utilization, still have the waste on certain resource.
Acetone and butanols are widely used in organic synthesis plastics, resin, paint, medicine and national defense industry.Acetone can be used as paint, cinefilm, plastics, petroleum refining dewaxing, vegetables oil extraction, the solvent of pharmacy and the raw material of synthetic organic chemical manufacturing industry.Butanols still is that a kind of potential is combined power fuel greatly except that can be used as solvent, and its fuel value and gasoline are suitable, are the substitutes of gasoline.Existing acetone, production of butanol technology have two kinds of methods, and a kind of is the petrochemical industry synthesis method, and another kind is a biological fermentation process.What petrochemical industry synthesized that the method raw material of acetone, butanols adopts is exhausted day by day petroleum resources, along with the minimizing petrochemical industry synthesis method of oil also will come to an end.It is corn that biological fermentation process is given birth to the raw material that adopts, and not only cost is too high, has also brought competitive pressure to feedstuff industry simultaneously.Utilize Maize Production acetone, butanols be directly corn screen, broken, propose embryo, gelatinization, connect the boiling that disappears etc. and handle in earlier stage, ferment, distill production acetone and butanols again.It is higher to utilize corn fermentation to produce the cost of method of acetone and butanols like this.
Summary of the invention
The purpose of this invention is to provide a kind of producing xylose with stalks cogeneration of propanone, butanols and alcoholic acid method utilized, realized utilization of resources maximization.
For achieving the above object, the present invention is by the following technical solutions: a kind of producing xylose with stalks cogeneration of propanone that utilizes, butanols and alcoholic acid method, crushed stalk adds diluted acid after with hot-water soak and is hydrolyzed, filtration is divided into xylose hydrolysis fluid and xylose residue, xylose hydrolysis fluid is through evaporation, decolouring, filter, ion-exchange, concentrate and crystallization production wood sugar, the hot water that in xylose residue, adds 35～60 ℃, the weight ratio of xylose residue and hot water is 1: 3～6, adding alkaline matter accent pH value is 4～7, add cellulase more in batches, every gram xylose residue adds 200～600ncu cellulase, then 40～55 ℃ of following continuous enzymolysis 60～120 hours; The enzymolysis membrane sepn that finishes filters and to be divided into decomposed solution and filter residue, and filter residue returns the continuation enzymolysis; In parting liquid, add nitrogenous source class material, the mass percent concentration of adjusting sugar in the parting liquid is 4～6%, carries out cooking disinfection 30～45 minutes under 120～150 ℃ then, sends into fermentor tank after being cooled to 35～41 ℃, the inoculation bacterial classification was 34～39 ℃ of following anaerobically fermentings 36～72 hours; Fermented liquid carries out rectifying and tells acetone, butanols and ethanol.
After the described crushed stalk with 60～90 ℃ of hot-water soaks 1～3 hour.
Described diluted acid is that mass percent concentration is 0.3～6% dilute sulphuric acid or dilute hydrochloric acid, and the add-on of diluted acid is 5～8 times of stalk weight.
Temperature is 100～130 ℃ during described hydrolysis, and pressure is 0.1～0.3MPa during hydrolysis, and hydrolysis time is 3～8 hours.
Described bacterial classification is clostridium acetobutylicum, acetone Clostridium butylicum, clostridium saccharobutyricum or Pasteur's gemma clostridium.
Described nitrogenous source class material is ammonium sulfate, monoammonium sulfate, ammonium chloride, dregs of beans, cottonseed meal, peanut meal, corn or rapeseed meal, and the add-on of nitrogenous source class material is 1～5% of a decomposed solution weight.
Described alkaline matter is calcium hydroxide, lime carbonate, yellow soda ash, sodium hydroxide, salt of wormwood or potassium hydroxide.
Slightly heat up in a steamer earlier before the described rectification working process.
Use the enzymolysis producer during described enzymolysis.
Described stalk is one or more in maize straw, wheat stalk, broomcorn straw, Sunflower Receptacle stalk, straw, corn cob, bagasse, Pericarppium arachidis hypogaeae or the cotton seed hulls.
Raw material used in the present invention is a straw-like materials, straw-like materials mainly is to be become to be grouped into for three kinds by hemicellulose, Mierocrystalline cellulose and xylogen, the main moiety of hemicellulose material is an xylan, the hemicellulose material is difficult for by cellulase hydrolysis, and the main moiety of Mierocrystalline cellulose is a hexosan, and Mierocrystalline cellulose is difficult for by dilute acid hydrolysis again.In order to utilize the Mierocrystalline cellulose straw-like materials fully, the present invention uses the hot-water soak stalk earlier, remove the material that pectin in the raw material and tannin etc. hinder hydrolysis, utilize diluted acid to make hydrolysis of hemicellulose be converted into pentose then, pentose is the good raw material of producing wood sugar, Xylitol or furfural; And under equal conditions hemicellulose acid hydrolysis speed is 100 times of cellulosic acid hydrolysis rate, be difficult for then being generated monose-glucose with a spot of cellulase hydrolysis by acid-hydrolyzed Mierocrystalline cellulose, so not only reduced the consumption of cellulase, also improved cellulosic utilization ratio greatly, add strain fermentation in the glucose and obtain acetone, butanols and alcoholic acid mixture, rectifying is with mixture separation.The present invention utilizes producing xylose with stalks cogeneration of propanone, butanols and alcoholic acid method to realize the maximization that the Mierocrystalline cellulose straw-like materials utilizes, not only utilize the straw resource fully, realized turning waste into wealth, improved value-added content of product, realized its economic worth, simultaneously also new raw material resources and method have been opened up, for enterprise and society have brought more economic benefit and social benefit for the production of acetone, butanols.
Embodiment
Embodiment 1: corn cob is pulverized back with 90 ℃ of hot-water soaks 1 hour, adding mass percent concentration then and be 0.3% dilute sulphuric acid is hydrolyzed, the dilute sulphuric acid add-on is 8 times of corn cob weight, temperature is 100 ℃ during hydrolysis, pressure is 0.1MPa during hydrolysis, hydrolysis time is 8 hours, filters to be divided into xylose hydrolysis fluid and xylose residue, and xylose hydrolysis fluid is through evaporation, decolouring, filtration, ion-exchange, concentrated and crystallization production wood sugar; The hot water that in xylose residue, adds 35 ℃, the weight ratio of xylose residue and hot water is 1: 6, and adding calcium hydroxide accent pH value is 4, adds cellulase more in batches, every gram xylose residue adds the 200ncu cellulase, sends into the enzymolysis producer then 40 ℃ of following continuous enzymolysis 120 hours; The enzymolysis membrane sepn that finishes filters and to be divided into decomposed solution and filter residue, and filter residue returns the continuation enzymolysis; In parting liquid, add ammonium sulfate, the add-on of ammonium sulfate is 1% of a decomposed solution weight, concentrating the mass percent concentration of adjusting sugar in the parting liquid is 6%, under 120 ℃, carried out cooking disinfection 45 minutes then, send into fermentor tank after being cooled to 35 ℃, inoculation clostridium acetobutylicum bacterial classification was 34 ℃ of following anaerobically fermentings 72 hours; Fermented liquid slightly heats up in a steamer earlier and carries out rectifying again and tell acetone, butanols and ethanol, and acetone, butanols, alcoholic acid mass ratio are 27: 62.2: 10.1.
Embodiment 2: it is back with 80 ℃ of hot-water soaks 2 hours that maize straw, wheat stalk and broomcorn straw are mixed pulverizing, adding mass percent concentration then and be 3% dilute hydrochloric acid is hydrolyzed, the dilute hydrochloric acid add-on is maize straw, wheat stalk and broomcorn straw gross weight 7 times, temperature is 120 ℃ during hydrolysis, pressure is 0.2MPa during hydrolysis, hydrolysis time is 5 hours, filtration is divided into xylose hydrolysis fluid and xylose residue, and xylose hydrolysis fluid is through evaporation, decolouring, filtration, ion-exchange, concentrated and crystallization production wood sugar; The hot water that in xylose residue, adds 45 ℃, the weight ratio of xylose residue and hot water is 1: 5.5, and adding lime carbonate accent pH value is 5, adds cellulase more in batches, every gram xylose residue adds the 400ncu cellulase, sends into the enzymolysis producer then 50 ℃ of following continuous enzymolysis 90 hours; The enzymolysis membrane sepn that finishes filters and to be divided into decomposed solution and filter residue, and filter residue returns the continuation enzymolysis; In parting liquid, add monoammonium sulfate, the add-on of monoammonium sulfate is 3% of a decomposed solution weight, concentrating the mass percent concentration of adjusting sugar in the parting liquid is 5%, under 140 ℃, carried out cooking disinfection 40 minutes then, send into fermentor tank after being cooled to 38 ℃, inoculation clostridium saccharobutyricum bacterial classification was 37 ℃ of following anaerobically fermentings 50 hours; Fermented liquid slightly heats up in a steamer earlier and carries out rectifying again and tell acetone, butanols and ethanol, and acetone, butanols, alcoholic acid mass ratio are 28: 61: 10.1.
Embodiment 3: pulverize Pericarppium arachidis hypogaeae and cotton seed hulls mixing back with 60 ℃ of hot-water soaks 3 hours, adding mass percent concentration then and be 6% dilute sulphuric acid is hydrolyzed, the dilute sulphuric acid add-on is Pericarppium arachidis hypogaeae and cotton seed hulls gross weight 5 times, temperature is 130 ℃ during hydrolysis, pressure is 0.3MPa during hydrolysis, hydrolysis time is 3 hours, filters to be divided into xylose hydrolysis fluid and xylose residue, and xylose hydrolysis fluid is through evaporation, decolouring, filtration, ion-exchange, concentrated and crystallization production wood sugar; The hot water that in xylose residue, adds 60 ℃, the weight ratio of xylose residue and hot water is 1: 3, and adding sodium hydroxide accent pH value is 7, adds cellulase more in batches, every gram xylose residue adds the 500ncu cellulase, sends into the enzymolysis producer then 55 ℃ of following continuous enzymolysis 60 hours; The enzymolysis membrane sepn that finishes filters and to be divided into decomposed solution and filter residue, and filter residue returns the continuation enzymolysis; In parting liquid, add corn, the add-on of corn is 2% of a decomposed solution weight, the mass percent concentration that adds sugar in the water adjustment parting liquid is 4%, under 150 ℃, carried out cooking disinfection 30 minutes then, send into fermentor tank after being cooled to 41 ℃, inoculation clostridium acetobutylicum bacterial classification was 39 ℃ of following anaerobically fermentings 36 hours; Fermented liquid slightly heats up in a steamer earlier and carries out rectifying again and tell acetone, butanols and ethanol, and acetone, butanols, alcoholic acid mass ratio are 27.4: 62: 10.1.
Embodiment 4: pulverize bagasse and straw mixing back with 70 ℃ of hot-water soaks 2.5 hours, adding mass percent concentration then and be 5% dilute hydrochloric acid is hydrolyzed, the dilute hydrochloric acid add-on is bagasse and straw gross weight 6 times, temperature is 110 ℃ during hydrolysis, pressure is 0.15MPa during hydrolysis, hydrolysis time is 6 hours, filters to be divided into xylose hydrolysis fluid and xylose residue, and xylose hydrolysis fluid is through evaporation, decolouring, filtration, ion-exchange, concentrated and crystallization production wood sugar; The hot water that in xylose residue, adds 50 ℃, the weight ratio of xylose residue and hot water is 1: 4, and adding yellow soda ash accent pH value is 6, adds cellulase more in batches, every gram xylose residue adds the 600ncu cellulase, sends into the enzymolysis producer then 45 ℃ of following continuous enzymolysis 100 hours; The enzymolysis membrane sepn that finishes filters and to be divided into decomposed solution and filter residue, and filter residue returns the continuation enzymolysis; In parting liquid, add dregs of beans, the add-on of dregs of beans is 5% of a decomposed solution weight, the mass percent concentration of adjusting sugar in the parting liquid is 5%, under 130 ℃, carried out cooking disinfection 35 minutes then, send into fermentor tank after being cooled to 40 ℃, inoculation Pasteur gemma clostridium species was 38 ℃ of following anaerobically fermentings 60 hours; Fermented liquid slightly heats up in a steamer earlier and carries out rectifying again and tell acetone, butanols and ethanol, and acetone, butanols, alcoholic acid mass ratio are 28.2: 61: 10.
More than with specific embodiment technical scheme of the present invention is described; but be not to be restriction to technical scheme of the present invention; those skilled in the art should understand that; still can make amendment or be equal to replacement invention; and not breaking away from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed among protection scope of the present invention.
Claims (10)
1, a kind of producing xylose with stalks cogeneration of propanone that utilizes, butanols and alcoholic acid method, crushed stalk adds diluted acid after with hot-water soak and is hydrolyzed, filtration is divided into xylose hydrolysis fluid and xylose residue, xylose hydrolysis fluid is through evaporation, decolouring, filter, ion-exchange, concentrate and crystallization production wood sugar, it is characterized in that: the hot water that in xylose residue, adds 35～60 ℃, the weight ratio of xylose residue and hot water is 1: 3～6, adding alkaline matter accent pH value is 4～7, add cellulase more in batches, every gram xylose residue adds 200～600ncu cellulase, then 40～55 ℃ of following continuous enzymolysis 60～120 hours; The enzymolysis membrane sepn that finishes filters and to be divided into decomposed solution and filter residue, and filter residue returns the continuation enzymolysis; In parting liquid, add nitrogenous source class material, the mass percent concentration of adjusting sugar in the parting liquid is 4～6%, carries out cooking disinfection 30～45 minutes under 120～150 ℃ then, sends into fermentor tank after being cooled to 35～41 ℃, the inoculation bacterial classification was 34～39 ℃ of following anaerobically fermentings 36～72 hours; Fermented liquid carries out rectifying and tells acetone, butanols and ethanol.
2, producing xylose with stalks cogeneration of propanone, butanols and the alcoholic acid method utilized as claimed in claim 1 is characterized in that: after the described crushed stalk with 60～90 ℃ of hot-water soaks 1～3 hour.
3, producing xylose with stalks cogeneration of propanone, butanols and the alcoholic acid method utilized as claimed in claim 2 is characterized in that: described diluted acid is that mass percent concentration is 0.3～6% dilute sulphuric acid or dilute hydrochloric acid, and the add-on of diluted acid is 5～8 times of stalk weight.
4, producing xylose with stalks cogeneration of propanone, butanols and the alcoholic acid method utilized as claimed in claim 3 is characterized in that: temperature is 100～130 ℃ during described hydrolysis, and pressure is 0.1～0.3MPa during hydrolysis, and hydrolysis time is 3～8 hours.
5, as each described producing xylose with stalks cogeneration of propanone, butanols and alcoholic acid method utilized of claim 1 to 4, it is characterized in that: described bacterial classification is clostridium acetobutylicum, acetone Clostridium butylicum, clostridium saccharobutyricum or Pasteur's gemma clostridium.
6, producing xylose with stalks cogeneration of propanone, butanols and the alcoholic acid method utilized as claimed in claim 5, it is characterized in that: described nitrogenous source class material is ammonium sulfate, monoammonium sulfate, ammonium chloride, dregs of beans, cottonseed meal, peanut meal, corn or rapeseed meal, and the add-on of nitrogenous source class material is 1～5% of a decomposed solution weight.
7, producing xylose with stalks cogeneration of propanone, butanols and the alcoholic acid method utilized as claimed in claim 6, it is characterized in that: described alkaline matter is calcium hydroxide, lime carbonate, yellow soda ash, sodium hydroxide, salt of wormwood or potassium hydroxide.
8, producing xylose with stalks cogeneration of propanone, butanols and the alcoholic acid method utilized as claimed in claim 7 is characterized in that: slightly heat up in a steamer earlier before the described rectification working process.
9, producing xylose with stalks cogeneration of propanone, butanols and the alcoholic acid method utilized as claimed in claim 8 is characterized in that: use the enzymolysis producer during described enzymolysis.
10, producing xylose with stalks cogeneration of propanone, butanols and the alcoholic acid method utilized as claimed in claim 9, it is characterized in that: described stalk is one or more in maize straw, wheat stalk, broomcorn straw, Sunflower Receptacle stalk, straw, corn cob, bagasse, Pericarppium arachidis hypogaeae or the cotton seed hulls.
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