CN107058405A - A kind of ecological, environmental protective bio-ethanol and preparation method thereof - Google Patents
A kind of ecological, environmental protective bio-ethanol and preparation method thereof Download PDFInfo
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- CN107058405A CN107058405A CN201710502839.9A CN201710502839A CN107058405A CN 107058405 A CN107058405 A CN 107058405A CN 201710502839 A CN201710502839 A CN 201710502839A CN 107058405 A CN107058405 A CN 107058405A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
Abstract
The invention discloses a kind of ecological, environmental protective bio-ethanol, include the raw material of following parts by weight：700 900 parts of solvent, 20 30 parts of rice straw, 15 25 parts of wheat stalk, 25 35 parts of maize straw, 5 15 parts of cotton stalk, 25 35 parts of rape stalk, 25 35 parts of bagasse, 10 20 parts of bran skin, 20 30 parts of corncob, 80 150 parts of annex solution of fermentation, 25 35 parts of lecithin, 10 20 parts of manganese sulfate.The preparation method that the present invention is provided uses a large amount of agricultural residues, material cost is relatively low, raw material is easy to get, realize recycling and comprehensively utilizing for vegetable material, reduce the pollution of environment, enzymolysis and hexose, the efficient progress of pentose fermentation successively simultaneously, the obtained rate of ethanol is higher, and technique is concise, with higher use value and good application prospect.
Description
Technical field
The present invention relates to biomass fuel technical field, and in particular to a kind of ecological, environmental protective bio-ethanol and its preparation side
Method.
Background technology
The energy is the basis of a national development economy, and energy shortage is also the key factor for restricting state economic development.
With the reinforcement of global resources, the energy, the protrusion of environmental problem and global warming trend, cleaning, regenerative resource are found
Only way as the mankind, wherein alcohol fuel turn into first choice by unique advantage.Due to grain alcohol development by
The restriction of each side such as soil, agricultural production, food supply and social economy, utilizes the strings such as abundant timber, dogstail
Raw material, especially cheap agriculture and forestry organic waste material produces alcohol fuel, i.e. cellulosic ethanol through bioconversion, as solve the energy and
One of effective way of environmental problem, is the focus of our times various countries research and development.
Lignocellulosic (Lignoccllulose) refer to nature growth, plant without any processing leaf,
The materials such as dry, stem, it contains cellulose, hemicellulose, lignin, protein, water, ash and graded material, on the earth it is annual about
100,000,000,000 tons of lignocellulosics are formed, are that distributed in nature is most wide, content is most, cheap and reproducible resource.
The agricultural wastes formed in China's only agricultural production, there are about 700,000,000 tons, the utilization rate of these discarded objects is very low every year, or even dirty
Contaminated environment, if the available energy can be translated into --- alcohol fuel, will be significant with it is wide before
Scape.
Fermentation of plant straws production ethanol typically uses two-step fermentation, i.e., first by pretreated plant cellulose material
Reduced sugar is hydrolyzed to, then recycles reduced sugar fermentation to obtain ethanol.Glucose and wood are mainly contained in straw hydrolysis sugar
Both ratios have very big difference in sugar, different hydrolysis sugars.Fermentation of plant straws production ethanol key is to improve thalline to carbohydrate thing
The utilization rate of matter, and then improve alcohol getting rate.
At this stage, the energy resource structure and the industrial structure of China all suffer from new adjustment and breakthrough, old energy industry knot
Structure using the new industry based on new, clean energy resource just by progressively to be substituted.Accelerate the hair of energy Engineering of Biomass project
Exhibition is the problem for being badly in need of research.It is badly in need of overcoming the regulation and control difficulty in fuel ethanol production is made using agricultural crop straw big, second
Alcohol low yield, production cost is high, is also difficult to the deficiency for putting into large-scale commercial fermentation.
The content of the invention
For the defect of prior art, it is an object of the invention to provide a kind of ecological, environmental protective bio-ethanol, the ecological, environmental protective
Bio-ethanol combustion rate is high, easily light, without black smoke, free from extraneous odour, it is environmentally safe, burn after residue it is few, while the present invention is carried
The preparation method of confession use a large amount of agricultural residues, material cost is relatively low, raw material is easy to get, realize vegetable material recycle with
Comprehensive utilization, reduce the pollution of environment, while successively enzymolysis and hexose, the efficient progress of pentose fermentation, ethanol be made rate compared with
Height, technique is concise, with higher use value and good application prospect.
The present invention solves technical problem and adopted the following technical scheme that：
The invention provides a kind of ecological, environmental protective bio-ethanol, include the raw material of following parts by weight：
700-900 parts of solvent, 20-30 parts of rice straw, 15-25 parts of wheat stalk, 25-35 parts of maize straw, cotton stalk 5-
15 parts, 25-35 parts of rape stalk, 25-35 parts of bagasse, 10-20 parts of bran skin, 20-30 parts of corncob, fermentation annex solution 80-
150 parts, 25-35 parts of lecithin, 10-20 parts of manganese sulfate.
Preferably, the ecological, environmental protective bio-ethanol includes the raw material of following parts by weight：
800 parts of solvent, 25 parts of rice straw, 20 parts of wheat stalk, 30 parts of maize straw, 10 parts of cotton stalk, rape stalk 30
Part, 30 parts of bagasse, 15 parts of bran skin, 25 parts of corncob, 110 parts of annex solution of fermentation, 30 parts of lecithin, 15 parts of manganese sulfate.
Preferably, the solvent is that formic acid solution, acetic acid solution, plant acid solution compare 15 according to weight:10:1 composition it is mixed
Compound；The oxalic acid solution, acetic acid solution, the concentration of plant acid solution are respectively 85%, 36%, 0.13%.
Preferably, the fermentation annex solution is cysteine, urea, agar, stearic acid, peptone, galactolipin, four water nitre
Sour calcium, potassium nitrate, diammonium hydrogen phosphate, niacinamide, water compare 2 according to weight:4:2.5:3:4.5:3:3.5:3:3.5:
2.5:200 mixed aqueous solution.
Preferably, the lecithin is the lecithin liquid that industrial concentration is 60%.
Present invention also offers a kind of preparation method of ecological, environmental protective bio-ethanol, comprise the following steps：
Step one, by rice straw, wheat stalk, maize straw, cotton stalk, rape stalk, bagasse, bran skin, corn
Core, dry, add in high speed disintegrator, be ground into particle of the particle diameter in 1-4cm, then hydrolysis reactor is added by the 70% of solvent
In be heated to 80-100 DEG C, input crushes the particle of sum, maintain the temperature at 120-130 DEG C hydrolyze 40-80 minute to hydrolyzing
Mixed liquor A afterwards；
Step 2, mixed liquor A made from step one is sent into countercurrent washer while hot, uses remaining solvent counter current washes, quilt
Washings is cellulose and hemicellulose, then washed with deionized water, cleans solvent, obtains purified cellulose and hemicellulose
Element；
Step 3, adds cellulase by purified cellulose made from step 2 and hemicellulose and mixes, and adds water, appropriate dilute sulphur
Acid, in 4.0-6.0, is sealed to substrate w/v concentration 5-15%, pH value with preservative film, under the conditions of 45-55 DEG C, and shaking table enzymolysis is anti-
48-72h is answered, reacted mixture centrifugal solid-liquid is separated, clear liquid is condensed into liquid glucose B through being evaporated under reduced pressure, and wherein glucose is dense
Spend for 100-200g/L；
Step 4, liquid glucose B made from step 3 is added 50%, the 60% of lecithin, the 70% of manganese sulfate of the annex solution that ferments, used
High temperature resistant type wine brewing high-activity yeast liquid anaerobic fermentation 36-48h under the conditions of 35-37 DEG C, ethanol is converted into by hexose, and decompression is steamed
Evaporate and obtain ethanol；Mash is added into remaining fermentation annex solution, lecithin, manganese sulfate；Again with pichia stipitis in 30-35 DEG C
Under the conditions of restricted oxygen supply fermentation liquid 60-80h, pentose is converted into ethanol, vacuum distillation obtains ethanol.
Preferably, the ecological, environmental protective bio-ethanol preparation process is：
Step one, by rice straw, wheat stalk, maize straw, cotton stalk, rape stalk, bagasse, bran skin, corn
Core, dry, add in high speed disintegrator, be ground into particle of the particle diameter in 1-4cm, then hydrolysis reactor is added by the 70% of solvent
In be heated to 90 DEG C, input crushes the particle of sum, maintain the temperature at 125 DEG C hydrolyze 60 minutes to the mixed liquor A after hydrolysis；
Step 2, mixed liquor A made from step one is sent into countercurrent washer while hot, uses remaining solvent counter current washes, quilt
Washings is cellulose and hemicellulose, then washed with deionized water, cleans solvent, obtains purified cellulose and hemicellulose
Element；
Step 3, adds cellulase by purified cellulose made from step 2 and hemicellulose and mixes, and adds water, appropriate dilute sulphur
Acid is to substrate w/v concentration 10%, and pH values are sealed 5.0 with preservative film, under the conditions of 50 DEG C, shaking table enzyme digestion reaction 60h, will be anti-
Mixture centrifugal solid-liquid separation after answering, clear liquid is condensed into liquid glucose B through being evaporated under reduced pressure, and wherein concentration of glucose is 150g/L；
Step 4, liquid glucose B made from step 3 is added 50%, the 60% of lecithin, the 70% of manganese sulfate of the annex solution that ferments, used
High temperature resistant type wine brewing high-activity yeast liquid anaerobic fermentation 42h under the conditions of 36 DEG C, is converted into ethanol, vacuum distillation is obtained by hexose
Ethanol；Mash is added into remaining fermentation annex solution, lecithin, manganese sulfate；Again with pichia stipitis in 33 DEG C of condition lower limits
Property oxygen supply fermentation liquid 70h processed, is converted into ethanol, vacuum distillation obtains ethanol by pentose.
Preferably, the addition of the cellulase in the step 2 is：12 FPIU fiber is added by every gram of cellulose
Plain enzyme；The cellulase is cellulase, β-glucuroide, zytase by weight 1:1.25:6.35 compositions it is mixed
Synthase.
Compared with prior art, the present invention has following beneficial effect：
（1）A kind of ecological, environmental protective bio-ethanol of the present invention is pre-processed to raw material application acid flux material, plant biomass
Hydrolyzed in course of dissolution, lignin is completely dissolved, cellulose, hemicellulose are not dissolved.Solvent is easily recycled in itself
It can reuse, the separation of the component of lignocellulose raw material three maximum yield enables lignocellulosic to comprehensively utilize, greatly increased
The efficiency reacted during follow-up ferment treatment.
（2）A kind of ecological, environmental protective bio-ethanol of the present invention with the addition of lecithin and manganese sulfate, can effectively improve ethanol
Obtained rate and yield, improve the ethanol production of ton plant fiber material, reduce the cost of material of ton ethanol.
（3）A kind of ecological, environmental protective bio-ethanol major ingredient of the present invention employs the waste materials such as substantial amounts of agricultural stalk, on the one hand
The cost of material is reduced, resource is make use of to greatest extent, sustainable development is realized, on the other hand efficiently solves rural area
Stalk waste material directly burns the pollution to air of pernicious gas and solid particulate matter of generation, protects environment.
（4）The sequential fermentation used in a kind of preparation method of ecological, environmental protective bio-ethanol of the present invention has taken into account high concentration
Zymohexose and pentose fermentation, the high concentration zymohexose after clear liquid concentration is at industrial scale without stirring, its height obtained
The mash of concentration of alcohol can reduce subsequent ethanol distillation steam consumption, and zymohexose high ethano yield ensure that it is high
The ethanol production of ton plant fiber material；Follow-up pentose fermentation further increases ethanol production, reduces cost of material, increases
Add the ethanol of the raw material of Unit Weight that rate is made, saved resource, protected environment.
（5）A kind of ecological, environmental protective bio-ethanol of the present invention preparation method cost is relatively low, raw material is easy to get, realize plant
Recycling and comprehensively utilizing for material, reduces the pollution of environment, at the same successively enzymolysis and hexose, pentose fermentation efficiently enter
OK, the obtained rate of ethanol is higher, and technique is concise, with higher use value and good application prospect.
Embodiment
With reference to specific embodiment, the technical scheme in the embodiment of the present invention is clearly and completely described, shown
So, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on the reality in the present invention
Example is applied, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made all belongs to
In the scope of protection of the invention.
Embodiment 1.
The ecological, environmental protective bio-ethanol of the present embodiment, includes the raw material of following parts by weight：
700 parts of solvent, 20 parts of rice straw, 15 parts of wheat stalk, 25 parts of maize straw, 5 parts of cotton stalk, rape stalk 25
Part, 25 parts of bagasse, 10 parts of bran skin, 20 parts of corncob, 80 parts of annex solution of fermentation, 25 parts of lecithin, 10 parts of manganese sulfate.
Solvent is that formic acid solution, acetic acid solution, plant acid solution compare 15 according to weight in the present embodiment:10:The mixing of 1 composition
Thing；The oxalic acid solution, acetic acid solution, the concentration of plant acid solution are respectively 85%, 36%, 0.13%.
The annex solution that fermented in the present embodiment is cysteine, urea, agar, stearic acid, peptone, galactolipin, four water nitre
Sour calcium, potassium nitrate, diammonium hydrogen phosphate, niacinamide, water compare 2 according to weight:4:2.5:3:4.5:3:3.5:3:3.5:2.5:
200 mixed aqueous solution.
Lecithin is the lecithin liquid that industrial concentration is 60% in the present embodiment.
The preparation method of the ecological, environmental protective bio-ethanol of the present embodiment, comprises the following steps：
Step one, by rice straw, wheat stalk, maize straw, cotton stalk, rape stalk, bagasse, bran skin, corn
Core, dry, add in high speed disintegrator, be ground into particle of the particle diameter in 1-4cm, then hydrolysis reactor is added by the 70% of solvent
In be heated to 80 DEG C, input crushes the particle of sum, maintain the temperature at 120 DEG C hydrolyze 80 minutes to the mixed liquor A after hydrolysis；
Step 2, mixed liquor A made from step one is sent into countercurrent washer while hot, uses remaining solvent counter current washes, quilt
Washings is cellulose and hemicellulose, then washed with deionized water, cleans solvent, obtains purified cellulose and hemicellulose
Element；
Step 3, adds cellulase by purified cellulose made from step 2 and hemicellulose and mixes, and adds water, appropriate dilute sulphur
Acid is to substrate w/v concentration 15%, and pH values are sealed 6.0 with preservative film, under the conditions of 55 DEG C, shaking table enzyme digestion reaction 48h, will be anti-
Mixture centrifugal solid-liquid separation after answering, clear liquid is condensed into liquid glucose B through being evaporated under reduced pressure, and wherein concentration of glucose is 100-200g/
L；
Step 4, liquid glucose B made from step 3 is added 50%, the 60% of lecithin, the 70% of manganese sulfate of the annex solution that ferments, used
High temperature resistant type wine brewing high-activity yeast liquid anaerobic fermentation 48h under the conditions of 35 DEG C, is converted into ethanol, vacuum distillation is obtained by hexose
Ethanol；Mash is added into remaining fermentation annex solution, lecithin, manganese sulfate；Again with pichia stipitis in 30 DEG C of condition lower limits
Property oxygen supply fermentation liquid 80h processed, is converted into ethanol, vacuum distillation obtains ethanol by pentose.
The addition of cellulase in the present embodiment step 2 is：12 FPIU cellulose is added by every gram of cellulose
Enzyme；The cellulase is cellulase, β-glucuroide, zytase by weight 1:1.25:The mixing of 6.35 compositions
Enzyme.
Embodiment 2.
The ecological, environmental protective bio-ethanol of the present embodiment, includes the raw material of following parts by weight：
900 parts of solvent, 30 parts of rice straw, 25 parts of wheat stalk, 35 parts of maize straw, 15 parts of cotton stalk, rape stalk 35
Part, 35 parts of bagasse, 20 parts of bran skin, 30 parts of corncob, 150 parts of annex solution of fermentation, 35 parts of lecithin, 20 parts of manganese sulfate.
Solvent is that formic acid solution, acetic acid solution, plant acid solution compare 15 according to weight in the present embodiment:10:The mixing of 1 composition
Thing；The oxalic acid solution, acetic acid solution, the concentration of plant acid solution are respectively 85%, 36%, 0.13%.
The annex solution that fermented in the present embodiment is cysteine, urea, agar, stearic acid, peptone, galactolipin, four water nitre
Sour calcium, potassium nitrate, diammonium hydrogen phosphate, niacinamide, water compare 2 according to weight:4:2.5:3:4.5:3:3.5:3:3.5:2.5:
200 mixed aqueous solution.
Lecithin is the lecithin liquid that industrial concentration is 60% in the present embodiment.
The preparation method of the ecological, environmental protective bio-ethanol of the present embodiment, comprises the following steps：
Step one, by rice straw, wheat stalk, maize straw, cotton stalk, rape stalk, bagasse, bran skin, corn
Core, dry, add in high speed disintegrator, be ground into particle of the particle diameter in 1-4cm, then hydrolysis reactor is added by the 70% of solvent
In be heated to 100 DEG C, input crushes the particle of sum, maintain the temperature at 130 DEG C hydrolyze 40 minutes to the mixed liquor after hydrolysis
A；
Step 2, mixed liquor A made from step one is sent into countercurrent washer while hot, uses remaining solvent counter current washes, quilt
Washings is cellulose and hemicellulose, then washed with deionized water, cleans solvent, obtains purified cellulose and hemicellulose
Element；
Step 3, adds cellulase by purified cellulose made from step 2 and hemicellulose and mixes, and adds water, appropriate dilute sulphur
Acid is to substrate w/v concentration 15%, and pH values are sealed 6.0 with preservative film, under the conditions of 55 DEG C, shaking table enzyme digestion reaction 48h, will be anti-
Mixture centrifugal solid-liquid separation after answering, clear liquid is condensed into liquid glucose B through being evaporated under reduced pressure, and wherein concentration of glucose is 100-200g/
L；
Step 4, liquid glucose B made from step 3 is added 50%, the 60% of lecithin, the 70% of manganese sulfate of the annex solution that ferments, used
High temperature resistant type wine brewing high-activity yeast liquid anaerobic fermentation 36h under the conditions of 37 DEG C, is converted into ethanol, vacuum distillation is obtained by hexose
Ethanol；Mash is added into remaining fermentation annex solution, lecithin, manganese sulfate；Again with pichia stipitis in 35 DEG C of condition lower limits
Property oxygen supply fermentation liquid 60h processed, is converted into ethanol, vacuum distillation obtains ethanol by pentose.
The addition of cellulase in the present embodiment step 2 is：12 FPIU cellulose is added by every gram of cellulose
Enzyme；The cellulase is cellulase, β-glucuroide, zytase by weight 1:1.25:The mixing of 6.35 compositions
Enzyme.
Embodiment 3.
The ecological, environmental protective bio-ethanol of the present embodiment, includes the raw material of following parts by weight：
800 parts of solvent, 25 parts of rice straw, 20 parts of wheat stalk, 30 parts of maize straw, 10 parts of cotton stalk, rape stalk 30
Part, 30 parts of bagasse, 15 parts of bran skin, 25 parts of corncob, 110 parts of annex solution of fermentation, 30 parts of lecithin, 15 parts of manganese sulfate.
Solvent is that formic acid solution, acetic acid solution, plant acid solution compare 15 according to weight in the present embodiment:10:The mixing of 1 composition
Thing；The oxalic acid solution, acetic acid solution, the concentration of plant acid solution are respectively 85%, 36%, 0.13%.
The annex solution that fermented in the present embodiment is cysteine, urea, agar, stearic acid, peptone, galactolipin, four water nitre
Sour calcium, potassium nitrate, diammonium hydrogen phosphate, niacinamide, water compare 2 according to weight:4:2.5:3:4.5:3:3.5:3:3.5:2.5:
200 mixed aqueous solution.
Lecithin is the lecithin liquid that industrial concentration is 60% in the present embodiment.
The preparation method of the ecological, environmental protective bio-ethanol of the present embodiment, comprises the following steps：
Step one, by rice straw, wheat stalk, maize straw, cotton stalk, rape stalk, bagasse, bran skin, corn
Core, dry, add in high speed disintegrator, be ground into particle of the particle diameter in 1-4cm, then hydrolysis reactor is added by the 70% of solvent
In be heated to 90 DEG C, input crushes the particle of sum, maintain the temperature at 125 DEG C hydrolyze 60 minutes to the mixed liquor A after hydrolysis；
Step 2, mixed liquor A made from step one is sent into countercurrent washer while hot, uses remaining solvent counter current washes, quilt
Washings is cellulose and hemicellulose, then washed with deionized water, cleans solvent, obtains purified cellulose and hemicellulose
Element；
Step 3, adds cellulase by purified cellulose made from step 2 and hemicellulose and mixes, and adds water, appropriate dilute sulphur
Acid is to substrate w/v concentration 10%, and pH values are sealed 5.0 with preservative film, under the conditions of 50 DEG C, shaking table enzyme digestion reaction 60h, will be anti-
Mixture centrifugal solid-liquid separation after answering, clear liquid is condensed into liquid glucose B through being evaporated under reduced pressure, and wherein concentration of glucose is 150g/L；
Step 4, liquid glucose B made from step 3 is added 50%, the 60% of lecithin, the 70% of manganese sulfate of the annex solution that ferments, used
High temperature resistant type wine brewing high-activity yeast liquid anaerobic fermentation 42h under the conditions of 36 DEG C, is converted into ethanol, vacuum distillation is obtained by hexose
Ethanol；Mash is added into remaining fermentation annex solution, lecithin, manganese sulfate；Again with pichia stipitis in 33 DEG C of condition lower limits
Property oxygen supply fermentation liquid 70h processed, is converted into ethanol, vacuum distillation obtains ethanol by pentose.
The addition of cellulase in the present embodiment step 2 is：12 FPIU cellulose is added by every gram of cellulose
Enzyme；The cellulase is cellulase, β-glucuroide, zytase by weight 1:1.25:The mixing of 6.35 compositions
Enzyme.
The result test result that various embodiments above prepares ethanol is as follows：
| Experimental project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Prior art |
| Ethanol content %（V/V） | 16.75 | 16.48 | 16.53 | 15.46 |
| Rate is made in ethanol | 0.50 | 0.51 | 0.51 | 0.49 |
| Residual sugar rate % (W/V) | 0.08 | 0.09 | 0.08 | 0.1 |
A kind of ecological, environmental protective bio-ethanol combustion rate of the present invention is high, easily light, without black smoke, free from extraneous odour, environmentally safe, combustion
Residue is few after burning, and the preparation method that the present invention is provided uses a large amount of agricultural residues, and material cost is relatively low, raw material is easy to get, and realizes
Recycling and comprehensively utilizing for vegetable material, reduces the pollution of environment, while enzymolysis and hexose, the height of pentose fermentation successively
Effect is carried out, and the obtained rate of ethanol is higher, and technique is concise, with higher use value and good application prospect.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It may be appreciated other embodiment.
Claims (8)
1. a kind of ecological, environmental protective bio-ethanol, it is characterised in that include the raw material of following parts by weight：
700-900 parts of solvent, 20-30 parts of rice straw, 15-25 parts of wheat stalk, 25-35 parts of maize straw, cotton stalk 5-
15 parts, 25-35 parts of rape stalk, 25-35 parts of bagasse, 10-20 parts of bran skin, 20-30 parts of corncob, fermentation annex solution 80-
150 parts, 25-35 parts of lecithin, 10-20 parts of manganese sulfate.
2. a kind of ecological, environmental protective bio-ethanol according to claim 1, it is characterised in that the ecological, environmental protective bio-ethanol
Include the raw material of following parts by weight：
800 parts of solvent, 25 parts of rice straw, 20 parts of wheat stalk, 30 parts of maize straw, 10 parts of cotton stalk, rape stalk 30
Part, 30 parts of bagasse, 15 parts of bran skin, 25 parts of corncob, 110 parts of annex solution of fermentation, 30 parts of lecithin, 15 parts of manganese sulfate.
3. a kind of ecological, environmental protective bio-ethanol according to claim 1 or 2, it is characterised in that the solvent is that formic acid is molten
Liquid, acetic acid solution, plant acid solution compare 15 according to weight:10:The mixture of 1 composition；The oxalic acid solution, acetic acid solution, phytic acid
The concentration of solution is respectively 85%, 36%, 0.13%.
4. a kind of ecological, environmental protective bio-ethanol according to claim 1 or 2, it is characterised in that the fermentation annex solution is
Cysteine, urea, agar, stearic acid, peptone, galactolipin, four water-calcium nitrate, potassium nitrate, diammonium hydrogen phosphate, niacinamide,
Water compares 2 according to weight:4:2.5:3:4.5:3:3.5:3:3.5:2.5:200 mixed aqueous solution.
5. a kind of ecological, environmental protective bio-ethanol according to claim 1 or 2, it is characterised in that the lecithin is industry
With the lecithin liquid that concentration is 60%.
6. a kind of method for preparing ecological, environmental protective bio-ethanol as claimed in claim 1 or 2, it is characterised in that including following
Step：
Step one, by rice straw, wheat stalk, maize straw, cotton stalk, rape stalk, bagasse, bran skin, corn
Core, dry, add in high speed disintegrator, be ground into particle of the particle diameter in 1-4cm, then hydrolysis reactor is added by the 70% of solvent
In be heated to 80-100 DEG C, input crushes the particle of sum, maintain the temperature at 120-130 DEG C hydrolyze 40-80 minute to hydrolyzing
Mixed liquor A afterwards；
Step 2, mixed liquor A made from step one is sent into countercurrent washer while hot, uses remaining solvent counter current washes, quilt
Washings is cellulose and hemicellulose, then washed with deionized water, cleans solvent, obtains purified cellulose and hemicellulose
Element；
Step 3, adds cellulase by purified cellulose made from step 2 and hemicellulose and mixes, and adds water, appropriate dilute sulphur
Acid, in 4.0-6.0, is sealed to substrate w/v concentration 5-15%, pH value with preservative film, under the conditions of 45-55 DEG C, and shaking table enzymolysis is anti-
48-72h is answered, reacted mixture centrifugal solid-liquid is separated, clear liquid is condensed into liquid glucose B through being evaporated under reduced pressure, and wherein glucose is dense
Spend for 100-200g/L；
Step 4, liquid glucose B made from step 3 is added 50%, the 60% of lecithin, the 70% of manganese sulfate of the annex solution that ferments, used
High temperature resistant type wine brewing high-activity yeast liquid anaerobic fermentation 36-48h under the conditions of 35-37 DEG C, ethanol is converted into by hexose, and decompression is steamed
Evaporate and obtain ethanol；Mash is added into remaining fermentation annex solution, lecithin, manganese sulfate；Again with pichia stipitis in 30-35 DEG C
Under the conditions of restricted oxygen supply fermentation liquid 60-80h, pentose is converted into ethanol, vacuum distillation obtains ethanol.
7. a kind of preparation method of ecological, environmental protective bio-ethanol according to claim 6, it is characterised in that the preparation step
Suddenly it is：
Step one, by rice straw, wheat stalk, maize straw, cotton stalk, rape stalk, bagasse, bran skin, corn
Core, dry, add in high speed disintegrator, be ground into particle of the particle diameter in 1-4cm, then hydrolysis reactor is added by the 70% of solvent
In be heated to 90 DEG C, input crushes the particle of sum, maintain the temperature at 125 DEG C hydrolyze 60 minutes to the mixed liquor A after hydrolysis；
Step 2, mixed liquor A made from step one is sent into countercurrent washer while hot, uses remaining solvent counter current washes, quilt
Washings is cellulose and hemicellulose, then washed with deionized water, cleans solvent, obtains purified cellulose and hemicellulose
Element；
Step 3, adds cellulase by purified cellulose made from step 2 and hemicellulose and mixes, and adds water, appropriate dilute sulphur
Acid is to substrate w/v concentration 10%, and pH values are sealed 5.0 with preservative film, under the conditions of 50 DEG C, shaking table enzyme digestion reaction 60h, will be anti-
Mixture centrifugal solid-liquid separation after answering, clear liquid is condensed into liquid glucose B through being evaporated under reduced pressure, and wherein concentration of glucose is 150g/L,；
Step 4, liquid glucose B made from step 3 is added 50%, the 60% of lecithin, the 70% of manganese sulfate of the annex solution that ferments, used
High temperature resistant type wine brewing high-activity yeast liquid anaerobic fermentation 42h under the conditions of 36 DEG C, is converted into ethanol, vacuum distillation is obtained by hexose
Ethanol；Mash is added into remaining fermentation annex solution, lecithin, manganese sulfate；Again with pichia stipitis in 33 DEG C of condition lower limits
Property oxygen supply fermentation liquid 70h processed, is converted into ethanol, vacuum distillation obtains ethanol by pentose.
8. a kind of preparation method of ecological, environmental protective bio-ethanol according to claim 6 or 7, it is characterised in that the step
The addition of cellulase in rapid two is：12 FPIU cellulase is added by every gram of cellulose；The cellulase is fibre
The plain enzyme of dimension, β-glucuroide, zytase are by weight 1:1.25:The mixed enzyme of 6.35 compositions.
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| CN101358218A (en) * | 2008-09-22 | 2009-02-04 | 王建设 | Method for producing xylose coupled cogeneration of propanone, butanol and ethanol using stalk |
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| CN103100260A (en) * | 2011-11-10 | 2013-05-15 | 华东理工大学 | Efficient method for separating solid residue and product in lignocellulose fermented mash |
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2017
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|---|---|---|---|---|
| CN101358218A (en) * | 2008-09-22 | 2009-02-04 | 王建设 | Method for producing xylose coupled cogeneration of propanone, butanol and ethanol using stalk |
| CN101358214A (en) * | 2008-09-23 | 2009-02-04 | 王建设 | Method for producing furfural coupled cogeneration of propanone and butanol using stalk |
| CN103100260A (en) * | 2011-11-10 | 2013-05-15 | 华东理工大学 | Efficient method for separating solid residue and product in lignocellulose fermented mash |
| CN102660882A (en) * | 2012-05-17 | 2012-09-12 | 刘德江 | Method for producing cellulose and fuel ethanol by dissolving plant straw with solvent |
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