CN107183067A - Method for preparing seaweed plant growth regulator by integral biological enzymolysis method - Google Patents
Method for preparing seaweed plant growth regulator by integral biological enzymolysis method Download PDFInfo
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- CN107183067A CN107183067A CN201710426196.4A CN201710426196A CN107183067A CN 107183067 A CN107183067 A CN 107183067A CN 201710426196 A CN201710426196 A CN 201710426196A CN 107183067 A CN107183067 A CN 107183067A
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- enzymolysis
- marine alga
- seaweed
- enzymatic
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- 238000000034 method Methods 0.000 title claims abstract description 56
- 241001474374 Blennius Species 0.000 title claims abstract description 55
- 239000005648 plant growth regulator Substances 0.000 title abstract description 5
- 239000003337 fertilizer Substances 0.000 claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 15
- 108090000790 Enzymes Proteins 0.000 claims abstract description 15
- 102000018997 Growth Hormone Human genes 0.000 claims abstract description 13
- 108010051696 Growth Hormone Proteins 0.000 claims abstract description 13
- 230000008569 process Effects 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 238000000926 separation method Methods 0.000 claims abstract description 11
- 239000012535 impurity Substances 0.000 claims abstract description 10
- 230000004913 activation Effects 0.000 claims abstract description 8
- -1 primary enzymolysis Substances 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 239000002002 slurry Substances 0.000 claims description 27
- 230000002255 enzymatic effect Effects 0.000 claims description 24
- 239000003630 growth substance Substances 0.000 claims description 20
- 229940088598 enzyme Drugs 0.000 claims description 14
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- 229920001282 polysaccharide Polymers 0.000 claims description 10
- 239000005017 polysaccharide Substances 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims description 9
- 108010059892 Cellulase Proteins 0.000 claims description 9
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 229940106157 cellulase Drugs 0.000 claims description 9
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 239000000084 colloidal system Substances 0.000 claims description 8
- 230000010355 oscillation Effects 0.000 claims description 8
- 238000004062 sedimentation Methods 0.000 claims description 8
- 239000004382 Amylase Substances 0.000 claims description 7
- 102000013142 Amylases Human genes 0.000 claims description 7
- 108010065511 Amylases Proteins 0.000 claims description 7
- 235000019418 amylase Nutrition 0.000 claims description 7
- RJYMRRJVDRJMJW-UHFFFAOYSA-L dibromomanganese Chemical compound Br[Mn]Br RJYMRRJVDRJMJW-UHFFFAOYSA-L 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 239000005711 Benzoic acid Substances 0.000 claims description 6
- 235000010233 benzoic acid Nutrition 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 6
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 6
- 238000005516 engineering process Methods 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 4
- 239000002152 aqueous-organic solution Substances 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims description 3
- 238000011105 stabilization Methods 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims 3
- 150000004676 glycans Chemical class 0.000 claims 2
- 241000372132 Hydrometridae Species 0.000 claims 1
- 101710128967 Somatotropin-1 Proteins 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 15
- 238000001994 activation Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 4
- 238000004140 cleaning Methods 0.000 abstract description 3
- 238000000227 grinding Methods 0.000 abstract description 3
- 238000010008 shearing Methods 0.000 abstract description 3
- 238000011282 treatment Methods 0.000 abstract description 3
- 239000013543 active substance Substances 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 238000000265 homogenisation Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 26
- 241000220223 Fragaria Species 0.000 description 13
- 235000016623 Fragaria vesca Nutrition 0.000 description 13
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 10
- 150000004804 polysaccharides Chemical class 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 7
- 241000195493 Cryptophyta Species 0.000 description 4
- 239000011149 active material Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 3
- 238000002203 pretreatment Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000002604 ultrasonography Methods 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- 239000000783 alginic acid Substances 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 229960001126 alginic acid Drugs 0.000 description 2
- 150000004781 alginic acids Chemical class 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000021053 average weight gain Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 239000003752 hydrotrope Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- QUCDWLYKDRVKMI-UHFFFAOYSA-M sodium;3,4-dimethylbenzenesulfonate Chemical compound [Na+].CC1=CC=C(S([O-])(=O)=O)C=C1C QUCDWLYKDRVKMI-UHFFFAOYSA-M 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 238000010129 solution processing Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 208000016261 weight loss Diseases 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a method for preparing a seaweed plant growth regulator by an integral biological enzymolysis method, which comprises nine steps of preparation before production, pretreatment of raw materials, primary enzymolysis, impurity removal of an enzymolysis solution, homogenization of the enzymolysis solution, secondary enzymolysis, post-treatment, separation and activation of somatotropin, final preparation of a fertilizer and the like. The invention utilizes the commercial compound enzyme to carry out enzymolysis on the seaweed, overcomes the complexity of pretreatment of the enzymolysis process such as seaweed soaking, cleaning, impurity removal, shearing, grinding and the like in the prior art and the defect of loss of active substances caused by the pretreatment, directly carries out moderate and efficient softening and enzymolysis on the whole fresh seaweed or dry seaweed, reserves the active substances in the seaweed to the maximum extent, and ensures the effect and the quality of the seaweed fertilizer.
Description
Technical field
Seaweed plants growth regulating is prepared the present invention relates to agricultural fertilizer field, more particularly to a kind of overall biologic enzymolysis method
The method of agent.
Background technology
Utilized extensively by the people on the ground such as West Europe early in the middle of century alga fertilizer of Christian era 12.The 17th century French Government is energetically
Recommend to be used as soil and fertilizer with marine alga in coastal area.There is within 1880 scholar to report the contrast using marine algae extract for the first time
Result of the test, clearly illustrate that marine alga as the superiority of fertilizer.Effect and research is widely applied to show, marine algae extract should
There is lot of advantages for crop production：Percentage of seedgermination can be improved, promotes growth of seedling, improves the yield and quality, increase is made
Thing resistance and the formation for promoting agron after crop harvesting.These fertilizer efficiency protruded are depended on naturally to be contained in marine alga
Abundant plant growth regulator such as cytokinin, auximone, gibberellin, abscisic acid, ethene, glycine betaine, polyamines
Deng, in addition, in marine alga also contain algal polysaccharides, alginic acid, highly unsaturated fatty acid and land specific to marine organisms
The mineral elements such as the rare zinc of plant, bromine, iodine, and substantial amounts of non-nitrogen containing organic matter and a number of amino acid, albumen
Outside matter and micronutrient element.From or so nineties in last century, external alga fertilizer pours in domestic market and driven China domestic
Marine alga is approved by agriculture personage always in application and development agriculturally, alga fertilizer in protrusion effect agriculturally and effect.
The Ministry of Agriculture proposes within particularly 2014《To the year two thousand twenty applying quantity of chemical fertilizer zero growth rate action plan》, the alga fertilizer conduct of environment-friendly high-efficiency
One kind of new-type fertilizer is increasingly favored and received by industry.
, it is necessary to extract alginic cell inclusion by clasmatosis or solubilization technique in alga fertilizer production, while making big point
Sub- mass degradation is solvable and easy absorbed small-molecule substance.Common methods have Physical such as Mechanical Crushing, low temperature explosion etc.,
This method environmental protection, can at utmost retain the active component in marine alga, but high to equipment requirement；Chemical method such as extraction
It is the main method of current production alginic acid, but has certain destruction to the active component in marine alga, HTHP strong acid is strong
The improper carbonization phenomenon for organic matter easily occur is controlled in alkali technical process；Biotechnology such as microbial fermentation, enzymatic isolation method etc., can
So that while marine alga active component is retained, its macromolecular to be converted into the small molecule that can be directly absorbed by crop, such as microorganism
Direct fermentation, moreover it is possible to produce the active component beneficial to crop not contained in other marine alga raw materials, but technical requirements are high, production
Cycle is long, product stability hardly possible control；Enzymatic isolation method is most efficient and gentle method, passes through selectivity enzyme preparation degraded macromolecular
Material, destruction cell release cell inclusion, gentle condition can farthest retain the active material of marine alga in itself.But
In existing zymolysis technique technological process, raw material marine alga is required for, by complicated pretreatment process, obtaining trickle of marine alga
Grain is in favor of the progress of enzymolysis.These pre-treatments include immersion, cleaning, removal of impurities, shearing, grinding of marine alga etc., a large amount of in marine alga
Active material such as mannitol, iodine, fucosan and other be lost in these pre-treating technologies, cause the activity of final products
Reduction.
Therefore, in the market needs a kind of biologic enzymolysis method to handle the side that overall marine alga prepares seaweed plants growth regulator
Method.
The content of the invention
To solve drawbacks described above present in prior art, the present invention is intended to provide a kind of utilize commodity complex enzyme to marine alga
Digested, overcome the complexity of enzymolysis process pre-treatment such as marine alga immersion, cleaning, removal of impurities, shearing, grinding etc. in conventional art
And the active material that brings of these pre-treatments is the drawbacks of be lost in, directly fresh seaweed or dry seaweed are integrally carried out gentle efficient
Soften and digest, farthest remain the active material in marine alga, it is ensured that the effect of alga fertilizer and the biology enzyme of quality
The method that the overall marine alga of solution processing prepares seaweed plants growth regulator.
In order to realize foregoing invention purpose, the present invention uses following technical scheme：A kind of overall biologic enzymolysis method prepares sea
The method of algae plant growth regulator, comprises the following steps：
1) prepare before producing
1. raw material are prepared：The complete fresh seaweed of preparation or dry seaweed, cellulase, algal polysaccharide enzyme, protease, pectin
Enzyme, amylase；
2. equipment and frock are prepared：Enzymatic vessel, colloid mill or homogenizer, the sonic oscillation for preparing to be provided with temperature control device are set
Standby, horizontal spiral discharge sedimentation centrifuge；
3. preparatory technology auxiliary material：Single ethylol amine, pentacarbonyl manganous bromide, benzoic acid；
2) pretreatment of raw material：
If new fresh seaweed is then integrally soaked in the water of 1-2 times of weight by fresh seaweed loads enzymatic vessel, contain in its reclaimed water
There are fresh seaweed weight 0.3%-0.8% inorganic salts and be well mixed, it is right under conditions of 40-60 DEG C of temperature with intermittence stirring
Marine alga carries out softening 1-3h, obtains the marine alga slurries of pH6.5-pH7.5 a kind of；
If dry seaweed is then integrally soaked in enzymatic vessel in the water of 5-10 times of weight by dry seaweed, dry sea is contained in its reclaimed water
Algae weight 3%-8% inorganic salts are simultaneously well mixed, and marine alga are carried out under conditions of 40-60 DEG C of temperature with intermittence stirring soft
Change 1-3h, obtain the marine alga slurries of pH6.5-pH7.5 a kind of.
3) one-level is digested
By temperature control at 35-50 DEG C, the cellulase of the 1-4% in terms of marine alga dry weight, 0.1- are added in marine alga slurries
0.3% algal polysaccharide enzyme, batch type, enzymolysis time 6-12h terminates one-level enzymolysis during to enzymolysis liquid pH5-6；
4) enzymolysis liquid removal of impurities
In one-level enzymolysis process, treat that the marine alga slurry viscosity of stiff is remarkably decreased, after can freely stirring, stirred with enzymolysis
Mix state, by enzymatic hydrolysis system between 2 enzymatic vessels multiple conversions, by sandstone settle carry out enzymatic hydrolysis system impurity removal；
5) enzymolysis liquid homogeneous
Step 3 will be completed) and step 4), substantially liquefied enzymolysis marine alga slurries, are carried out by colloid mill or homogenizer
Further micronization processes, make the marine alga particle not digested thoroughly be refined to 100nm-10 μm；
6) secondary enzymolysis
By step 5) obtain homogeneous enzymolysis liquid adjust to pH6.5-pH7.5, temperature adjustment to 50-60 DEG C, add marine alga
Dry weight 0.1%-0.3% protease, 1-3% pectase, 0.1%-0.2% amylase, batch type carries out two grades
Enzymolysis, enzymolysis time 2-4h；
7) post-process
By step 6) obtained enzymolysis liquid carries out separation of solid and liquid by horizontal spiral discharge sedimentation centrifuge, can obtain solid
Marine alga Enzymatic Extraction liquid of the thing content in 3-10%；
8) the separation activation of somatotropin
1. single ethylol amine is put into the marine alga Enzymatic Extraction liquid 7) obtained, then mixed concentrate is inserted into ultrasound
Oscillator device, with 300W-350W oscillation of power 35min-40min, obtains last slurry eventually；
2. last slurry eventually is stood to the aqueous solution and organic solution natural layering, isolates organic solution and the aqueous solution；
3. the pentacarbonyl manganous bromide and marine alga gross dry weight 1% of marine alga dry weight 1% will be added in the organic solution isolated
Benzoic acid, reaction finishes after stabilization, that is, the somatotropin of activation needed for obtaining；
9) fertilizer is matched somebody with somebody eventually
1. somatotropin step in 8) 3. obtained with 8) in the aqueous solution that 2. obtains of step remix, that is, needed for obtaining
Overall biologic enzymolysis method prepares seaweed plants growth regulator.
Compared with prior art, by adopting the above-described technical solution, the present invention has advantages below：That takes is most main
It is complete marine alga to want raw material, therefore effective ingredient recoverable amount is high, environment-friendly, and sustainable development to a greater extent can be achieved
Exhibition and the optimum use of resource；Production process science and it is efficient, the cellulase and algal polysaccharide enzyme first added is thin in plant
Its effect quickly, is thoroughly played under the full environment of born of the same parents' water, the cell membrane of plant cell is destroyed, releases in plants shootses
Various materials, the protease added afterwards, amylase and pectase decompose or cracked respectively various big in aquation these materials
Molecular substance, makes all composition molecular weight reductions, volume reduces, activity increases；Rationally can be molten respectively using water and organic solvent
Solve opposed polarity material characteristic and water and be partially insoluble in water organic solvent natural separation characteristic, isolate the hydrotrope and
Be dissolved in single ethylol amine, other organic matters is dissolved in single ethylol amine to Plant growth-promoting effect element less more in organic molten thing, recycling plant
Characteristic, realize the accurate extraction of somatotropin, then somatotropin is further through pentacarbonyl manganous bromide and benzoic acid activation process
Three kinds of characteristics are obtained afterwards, and one is not oxidizable when being contacted with the oxygen in air, solves somatotropin in routine techniques and is difficult to protect
The predicament deposited, two will not produce composition transfer, impact effect when being mixed with other conventional pesticides；Three be it is more affine with water, more
Easily absorbed by the hydrone in soil by root system of plant, absorptivity is higher than the plant growth regulator extracted in routine techniques.
Embodiment
Embodiment 1
A kind of method that overall biologic enzymolysis method prepares seaweed plants growth regulator, comprises the following steps：
1) prepare before producing
1. raw material are prepared：Prepare complete dry seaweed, cellulase, algal polysaccharide enzyme, protease, pectase, starch
Enzyme；
2. equipment and frock are prepared：Enzymatic vessel, colloid mill or homogenizer, the sonic oscillation for preparing to be provided with temperature control device are set
Standby, horizontal spiral discharge sedimentation centrifuge；
3. preparatory technology auxiliary material：Single ethylol amine, pentacarbonyl manganous bromide, benzoic acid；
2) pretreatment of raw material：
Dry seaweed is integrally soaked in enzymatic vessel in the water of 10 times of weight, the Asia containing dry seaweed weight 5% in its reclaimed water
Potassium sulfate is simultaneously well mixed, and is carried out softening 2.5h to marine alga under conditions of temperature 50 C with intermittent stirring, is obtained a kind of pH
About 7 marine alga slurries.
3) one-level is digested
By temperature control at 38 DEG C, add in terms of marine alga dry weight 2% cellulase in marine alga slurries, 0.1% it is brown
Polysaccharides enzyme, batch type, enzymolysis time 9h, now enzymolysis liquid pH5.5, terminates one-level enzymolysis；
4) enzymolysis liquid removal of impurities
In one-level enzymolysis process, treat that the marine alga slurry viscosity of stiff is remarkably decreased, after can freely stirring, stirred with enzymolysis
Mix state, by enzymatic hydrolysis system between 2 enzymatic vessels multiple conversions, by sandstone settle carry out enzymatic hydrolysis system impurity removal；
5) enzymolysis liquid homogeneous
Step 3 will be completed) and step 4), substantially liquefied enzymolysis marine alga slurries, are carried out by colloid mill or homogenizer
Further micronization processes, make the marine alga particle not digested thoroughly be refined to 1 μm；
6) secondary enzymolysis
By step 5) the homogeneous enzymolysis liquid that obtains adjusted to pH7, and temperature adjustment adds marine alga dry weight 0.1% to 60 DEG C
Protease, 1 pectase, 0.1% amylase, batch type carries out secondary enzymolysis, enzymolysis time 2h；
7) post-process
By step 6) obtained enzymolysis liquid carries out separation of solid and liquid by horizontal spiral discharge sedimentation centrifuge, can obtain solid
Marine alga Enzymatic Extraction liquid of the thing content 7%；
8) the separation activation of somatotropin
1. single ethylol amine is put into the marine alga Enzymatic Extraction liquid 7) obtained, then mixed concentrate is inserted into ultrasound
Oscillator device, with 350W oscillation of power 40min, obtains last slurry eventually；
2. last slurry eventually is stood to the aqueous solution and organic solution natural layering, isolates organic solution and the aqueous solution；
3. the pentacarbonyl manganous bromide and marine alga gross dry weight 1% of marine alga dry weight 1% will be added in the organic solution isolated
Benzoic acid, reaction finishes after stabilization, that is, the somatotropin of activation needed for obtaining；
9) fertilizer is matched somebody with somebody eventually
1. somatotropin step in 8) 3. obtained with 8) in the aqueous solution that 2. obtains of step remix, that is, needed for obtaining
Overall biologic enzymolysis method prepares seaweed plants growth regulator.
The overall biologic enzymolysis method produced according to the present embodiment prepares seaweed plants growth regulator, field fertilizer efficiency experiment feelings
Condition is：Experiment effect of the seaweed plants growth regulator on strawberry：
Test place：Qingdao of Shandong province Chengyang District Xia Zhuan
Experimental period：2 months in April, -2016 in 2016
Test kind：Greenhouse sweet tea treasured strawberry
Experimental design：Using cell method of comparison, the field management such as good ecological environmental condition, rich water and insect pest preventing and controlling are selected
Consistent plot.
Experiment sets 2 processing：(1) the seaweed plants growth regulator that the technique productions of embodiment 1 are obtained, is diluted with water 1000
Positive and negative foliage-spray again；(2) clear water is compareed, and the clear water of equivalent is applied in check plot.3 repetitions of each treatment group, each cell is random
Arrangement.
Fertilization mode：Since second batch of swollen fruiting period of fruit every 7 days positive and negative foliage-sprays once, co-administer 4 times.
Experiment effect
Influence of the seaweed plants growth regulator to strawberry fruit weight and sugariness
The strawberry fruit weight and fruit sweetness of table 1 harvest time different disposal group
After each experimental group is disposed according to experimental program, 10 strawberry progress are randomly selected respectively in each experiment repeating groups
Weigh and pol measure, average as each cell index.Each replicated plot index takes average to be each treatment group index again.From
It can see in data, after enzymolysis seaweed plants growth regulator, the fruit size of strawberry is substantially bigger than clear water control group,
Average weight gain 18.77%；The sugariness of strawberry compares clear water control group, increases bigger, average sugariness increase 31.4%.
Embodiment 2
A kind of method that overall biologic enzymolysis method prepares seaweed plants growth regulator, overall consistent with embodiment 1, difference
Part is：
2) pretreatment of raw material：
New fresh seaweed is integrally soaked in the water of 2 times of weight and loads enzymatic vessel, fresh seaweed weight is contained in its reclaimed water
0.3% inorganic salts are simultaneously well mixed, and are carried out softening 1h to marine alga under conditions of temperature 40 with intermittent stirring, are obtained one kind
PH6.5 marine alga slurries；
3) one-level is digested
By temperature control at 35 DEG C, add in terms of marine alga dry weight 4% cellulase in marine alga slurries, 0.3% it is brown
Polysaccharides enzyme, batch type, enzymolysis time 6h terminates one-level enzymolysis during to enzymolysis liquid pH5；
5) enzymolysis liquid homogeneous
Step 3 will be completed) and step 4), substantially liquefied enzymolysis marine alga slurries, are carried out by colloid mill or homogenizer
Further micronization processes, make the marine alga particle not digested thoroughly be refined to 100nm；
6) secondary enzymolysis
By step 5) the homogeneous enzymolysis liquid that obtains adjusted to pH6.5, and temperature adjustment adds marine alga dry weight 0.3% to 50 DEG C
Protease, 3% pectase, 0.2% amylase, batch type carry out secondary enzymolysis, enzymolysis time 4h；
7) post-process
By step 6) obtained enzymolysis liquid carries out separation of solid and liquid by horizontal spiral discharge sedimentation centrifuge, can obtain solid
Marine alga Enzymatic Extraction liquid of the thing content 10%；
8) the separation activation of somatotropin
1. single ethylol amine is put into the marine alga Enzymatic Extraction liquid 7) obtained, then mixed concentrate is inserted into ultrasound
Oscillator device, with 300W oscillation of power 35min, obtains last slurry eventually；
The overall biologic enzymolysis method produced according to the present embodiment prepares seaweed plants growth regulator, field fertilizer efficiency experiment feelings
Condition is：Experiment effect of the seaweed plants growth regulator on strawberry：The fruit size of strawberry is substantially bigger than clear water control group, puts down
Increase weight 21.23%；The sugariness of strawberry compares clear water control group, increases bigger, average sugariness increase 37.9%.
Embodiment 3
A kind of method that overall biologic enzymolysis method prepares seaweed plants growth regulator, overall consistent with embodiment 1, difference
Part is：
2) pretreatment of raw material：
Dry seaweed is integrally soaked in enzymatic vessel in the water of 5 times of weight, the sulfurous containing dry seaweed weight 5% in its reclaimed water
Sour potassium is simultaneously well mixed, and carries out softening 3h to marine alga under conditions of temperature 60 C with intermittent stirring, it is about 7 to obtain a kind of pH
Marine alga slurries.
3) one-level is digested
By temperature control at 50 DEG C, add in terms of marine alga dry weight 1% cellulase in marine alga slurries, 0.3% it is brown
Polysaccharides enzyme, batch type, enzymolysis time 6h, now enzymolysis liquid pH6.5, terminates one-level enzymolysis；
5) enzymolysis liquid homogeneous
Step 3 will be completed) and step 4), substantially liquefied enzymolysis marine alga slurries, are carried out by colloid mill or homogenizer
Further micronization processes, make the marine alga particle not digested thoroughly be refined to 10 μm；
6) secondary enzymolysis
By step 5) obtain homogeneous enzymolysis liquid adjust to pH7.5, temperature adjustment is to 50 DEG C；
7) post-process
By step 6) obtained enzymolysis liquid carries out separation of solid and liquid by horizontal spiral discharge sedimentation centrifuge, can obtain solid
Marine alga Enzymatic Extraction liquid of the thing content 3%；
The overall biologic enzymolysis method produced according to the present embodiment prepares seaweed plants growth regulator, field fertilizer efficiency experiment feelings
Condition is：Experiment effect of the seaweed plants growth regulator on strawberry：The fruit size of strawberry is substantially bigger than clear water control group, puts down
Increase weight 15.19%；The sugariness of strawberry compares clear water control group, increases bigger, average sugariness increase 29.6%.
To sum up experiment illustrates, the seaweed plants growth regulator effect obtained using fresh seaweed is better than dry seaweed.
The foregoing description of the disclosed embodiments, only for enabling professional and technical personnel in the field to realize or using this
Invention.A variety of modifications to these embodiments will be apparent for those skilled in the art, institute herein
The General Principle of definition can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore,
The present invention is not intended to be limited to the embodiments shown herein, and is to fit to special with principles disclosed herein and novelty
The consistent most wide scope of point.
Claims (1)
1. a kind of method that overall biologic enzymolysis method prepares seaweed plants growth regulator, it is characterised in that comprise the following steps：
1) prepare before producing
1. raw material are prepared：Prepare complete fresh seaweed or dry seaweed, cellulase, algal polysaccharide enzyme, protease, pectase,
Amylase；
2. equipment and frock are prepared：Prepare to be provided with enzymatic vessel, colloid mill or the homogenizer of temperature control device, sonic oscillation equipment,
Horizontal spiral discharge sedimentation centrifuge；
3. preparatory technology auxiliary material：Single ethylol amine, pentacarbonyl manganous bromide, benzoic acid；
2) pretreatment of raw material：
If new fresh seaweed is then integrally soaked in the water of 1-2 times of weight by fresh seaweed loads enzymatic vessel, containing fresh in its reclaimed water
Seaweed weight 0.3%-0.8% inorganic salts are simultaneously well mixed, to marine alga under conditions of 40-60 DEG C of temperature and intermittent stirring
Softening 1-3h is carried out, the marine alga slurries of pH6.5-pH7.5 a kind of are obtained；
If dry seaweed is then integrally soaked in enzymatic vessel in the water of 5-10 times of weight by dry seaweed, dry seaweed weight is contained in its reclaimed water
Measure 3%-8% inorganic salts and be well mixed, softening 1- is carried out to marine alga under conditions of 40-60 DEG C of temperature and intermittent stirring
3h, obtains the marine alga slurries of pH6.5-pH7.5 a kind of.
3) one-level is digested
By temperature control at 35-50 DEG C, the cellulase of the 1-4% in terms of marine alga dry weight, 0.1-0.3% are added in marine alga slurries
Algal polysaccharide enzyme, batch type, enzymolysis time 6-12h, terminate during to enzymolysis liquid pH5-6 one-level enzymolysis；
4) enzymolysis liquid removal of impurities
In one-level enzymolysis process, treat that the marine alga slurry viscosity of stiff is remarkably decreased, after can freely stirring, with enzymolysis stirring shape
State, by enzymatic hydrolysis system between 2 enzymatic vessels multiple conversions, by sandstone settle carry out enzymatic hydrolysis system impurity removal；
5) enzymolysis liquid homogeneous
Step 3 will be completed) and step 4), substantially liquefied enzymolysis marine alga slurries, traveling one is entered by colloid mill or homogenizer
Micronization processes are walked, the marine alga particle not digested thoroughly is refined to 100nm-10 μm；
6) secondary enzymolysis
By step 5) obtain homogeneous enzymolysis liquid adjust to pH6.5-pH7.5, temperature adjustment to 50-60 DEG C, add marine alga dry weight
0.1%-0.3% protease, 1-3% pectase, 0.1%-0.2% amylase, batch type carries out secondary enzymolysis,
Enzymolysis time 2-4h；
7) post-process
By step 6) obtained enzymolysis liquid carries out separation of solid and liquid by horizontal spiral discharge sedimentation centrifuge, and it can obtain solid content and contain
Measure the marine alga Enzymatic Extraction liquid in 3-10%；
8) the separation activation of somatotropin
1. single ethylol amine is put into the marine alga Enzymatic Extraction liquid 7) obtained, then mixed concentrate is inserted into sonic oscillation
Equipment, with 300W-350W oscillation of power 35min-40min, obtains last slurry eventually；
2. last slurry eventually is stood to the aqueous solution and organic solution natural layering, isolates organic solution and the aqueous solution；
3. by pentacarbonyl manganous bromide and the benzene first of marine alga gross dry weight 1% that marine alga dry weight 1% is added in the organic solution isolated
Acid, reaction finishes after stabilization, that is, the somatotropin of activation needed for obtaining；
9) fertilizer is matched somebody with somebody eventually
1. somatotropin step in 8) 3. obtained with 8) in the aqueous solution that 2. obtains of step remix, that is, it is overall needed for obtaining
Biologic enzymolysis method prepares seaweed plants growth regulator.
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