CN103102203B - Pleurotus eryngii culture medium as well as preparation method and application thereof - Google Patents
Pleurotus eryngii culture medium as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN103102203B CN103102203B CN201310069481.7A CN201310069481A CN103102203B CN 103102203 B CN103102203 B CN 103102203B CN 201310069481 A CN201310069481 A CN 201310069481A CN 103102203 B CN103102203 B CN 103102203B
- Authority
- CN
- China
- Prior art keywords
- pleurotus eryngii
- substratum
- culture medium
- water
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention provides a pleurotus eryngii culture medium which is characterized in that the culture medium adopts broad been skins as base materials and comprises water and a dry substance mixed with the water. The water content of the culture medium is 63-66%. The dry substance comprises the following components including the broad been skins, wood dust, wheat bran, corn powder, rice bran, bean pulp, lime powder, gypsum, calcium superphosphate and monopotassium phosphate. The invention further discloses a preparation method and application of the pleurotus eryngii culture medium. Compared with the prior art, the pleurotus eryngii culture medium has the advantages that the broad been skins and other components are organically combined together by adopting a reasonable ratio so as to provide sufficient nutrition and a good growing environment for pleurotus eryngii and guarantee the high yield and the high quality of the pleurotus eryngii; and meanwhile, the broad been skins are used for replacing the wood dust so that wastes can be recycled and the wastes are changed into valuable things, so as to be good for environmental friendliness.
Description
Technical field
The present invention relates to a kind of Pleurotus eryngii substratum, application Testa Viciae fabae is as matrix.The invention still further relates to preparation method and the concrete application of this substratum, belong to fungus growing technique field.
Background technology
Pleurotus eryngii, claims again eryngo to pick up the ears, its bacterial context plumpness, and quality is fresh and tender, nutritious, tasty, and there is good health-care effect, often edible Pleurotus eryngii, can reduce the cholesterol level in blood of human body, have certain adjusting blood pressure effect, can strengthen the immunizing power of human body.
Pleurotus eryngii is decomposition of cellulose and the stronger edible mushrooms of xylogen ability, and all the time, mushroom agriculture is all to use wood chip and cotton seed hulls as culturing raw material.But nowadays; problem becomes increasingly conspicuous with there is lack of raw materials in ecological damage, and one side, because the resources of production such as wood chip consumes excessively, damages to forestry ecology environment; cotton seed hulls price skyrockets on the other hand, has formed the cultivation crisis of resource of mushroom industry sustainable development.Therefore, for alleviating the Edible Fungi contradiction that consumes in a large number forest, circulate benignly as fundamental principle taking the resource of the ecological agriculture, seek the technology of utilizing of the alternate resources of Edible Fungi and science thereof, become the task that edible mushrooms industry is extremely urgent.
Broad bean, there is cultivation south of the River each province, is the main raw material of brewageing broad bean paste, and its seed coat-Testa Viciae fabae is the tankage of brewery, contains a large amount of xylogen, Mierocrystalline cellulose and hemicellulose.Along with the volume of production and marketing of broad bean fabricated product constantly increases, Testa Viciae fabae quantity also increases sharply in recent years, so the processing of Testa Viciae fabae and effective utilization have just been become to the problem of anxious research to be developed.Exploitation Testa Viciae fabae resource is not only conducive to promote the added value of Testa Viciae fabae, turns waste into wealth, and also significant aspect protection of the environment.
Summary of the invention
Technical problem to be solved by this invention has following 3 points:
One,, for above-mentioned present situation, provide a kind of Pleurotus eryngii substratum taking Testa Viciae fabae as main raw material.This substratum passes through Pleurotus eryngii mycelia by the Mierocrystalline cellulose in Testa Viciae fabae, hemicellulose and lignin degradation, conversion, utilization, thereby reaches the object that reduces costs, improves output.
Two, the preparation method of above-mentioned substratum.
Three, the application of above-mentioned substratum.
The present invention for solving the problems of the technologies described above adopted technical scheme is:
A kind of Pleurotus eryngii substratum, is characterized in that this substratum application Testa Viciae fabae is as matrix, and the dry-matter that comprises water and mix with water, and the water content of this substratum is 63-66%, and aforesaid dry-matter and mass percentage content thereof are as follows:
Testa Viciae fabae 35%-45%;
Wood chip 20%-30%;
Wheat bran 14%-18%;
Semen Maydis powder 6%-10%;
Rice bran 4%-6%;
Dregs of beans 2%-4%;
Lime powder 1.0%;
Gypsum 0.8%-1.2%;
Calcium superphosphate 0.4%-0.6%;
Potassium primary phosphate 0.4%-0.6%.
Testa Viciae fabae, through drying, is ground into the following particle of 5mm.The main component of Testa Viciae fabae is Mierocrystalline cellulose, hemicellulose, xylogen, still contains in addition certain mineral substance, protein, VITAMIN etc.Wherein Mierocrystalline cellulose 56.8%, hemicellulose 11.8%, xylogen 8.7%, crude protein 4.64%, structural protein 4.12%.
Dregs of beans is a kind of byproduct obtaining after soybean extracting bean oil, and the main component of dregs of beans is: protein 40%-48%, and Methionin 2.5%-3.0%, tryptophane 0.6%-0.7%, methionine(Met) 0.5%-0.7%, is the fine nutrilite of one of Pleurotus eryngii.
Testa Viciae fabae particle is thicker, after interpolation wood chip, changes its physical structure, has regulated the elasticity of substratum, makes planting material keep good air permeability.
Add appropriate wheat bran, rice bran, Semen Maydis powder, dregs of beans, both increased the nutrition of substratum, improved again the water-absorbing-retaining ability of substratum.
Lime powder is used for regulating pH value.
Gypsum, calcium superphosphate, potassium primary phosphate are all commercially available.
As preferably, the particle diameter of described Testa Viciae fabae is below 5mm.
A preparation method for Pleurotus eryngii substratum, is characterized in that comprising following processing step:
1. after Testa Viciae fabae being mixed with wood chip, prewet with liming, the 24h that banks up that prewets, makes the water content of mixture remain on 55%-60%;
2. wheat bran, Semen Maydis powder, rice bran, dregs of beans siccative are poured in stirrer, stirred, add step mixture 1. and stir again;
3. by gypsum, calcium superphosphate, potassium primary phosphate is added to the water dissolving, and the aqueous solution is added in said mixture, fully stirs 15-20min, makes the water content of substratum remain on 63%-66%, and pH value is 6.5-7.0;
4. bottle, beat and plant bacterium hole, sterilizing, cooling.
Because of Testa Viciae fabae and wood chip water-absorbent poor, first prewet with 1% lime aqueous solution, make Testa Viciae fabae and wood chip fully absorb moisture content.
Further, when 4. step bottles, stamp and plant bacterium hole, planting aperture, bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band.The effect of planting bacterium hole is the air permeability that increases substratum, increases bacterial classification contact surface, makes culture material send out up and down bacterium simultaneously, accelerates to send out bacterium speed.Step 4. in sterilizing and cooling conditions as follows: first keep 100 DEG C of sterilizing 1h, then rise to 121 DEG C of autoclaving 1.5h, after sterilizing, be cooled to below 25 DEG C.
Utilize the cultural method of Pleurotus eryngii substratum, after inoculation, 20 DEG C-22 DEG C of culturing room's temperature, relative humidity 60%-75%, incubation time is 23d-25d.
Compared with prior art, the invention has the advantages that: in Testa Viciae fabae culture material, added the culture material with nutrition and complementary structure, can provide sufficient nutrient and good growing environment to Pleurotus eryngii, ensured product high-yield high-quality; Utilize Testa Viciae fabae to replace wood chip simultaneously, make waste be able to recycling, turn waste into wealth, be beneficial to environmental protection.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1: the dry-matter that in the present embodiment, substratum comprises water and mixes with water, the water content of this substratum is 63-66%, and dry-matter component and proportioning are as follows: Testa Viciae fabae 45%, wood chip 20%, wheat bran 14%, Semen Maydis powder 10%, rice bran 4%, dregs of beans 4%, lime powder 1%, gypsum 1%, calcium superphosphate 0.5%, potassium primary phosphate 0.5%.
Testa Viciae fabae is ground into the particle below 5mm, and Testa Viciae fabae is mixed with wood chip, with the 1% liming 24h that banks up that prewets, makes the water content of mixture remain on 55%-60%; Wheat bran, Semen Maydis powder, rice bran, dregs of beans siccative are poured in stirrer, stirred, then add the mixture after prewetting, then stir; After finally gypsum, calcium superphosphate, potassium primary phosphate being dissolved in water, be spilled into, fully stir 15-20min, make mixture water content remain on 63～66%, pH value is 6.5-7.0.Pack in 1100ml × 78mmPP culture bottle with bottling machine, bottling requires to tighten lower pine, every bottle of weight 730g (wet feed), stamp and plant bacterium hole, aperture is 16mm, then covers the bottle cap of the ventilative sponge of band, then put into autoclave, first keep 100 DEG C of sterilizing 1h, then rise to 121 DEG C of autoclaving 1.5h, after sterilizing, be cooled to below 25 DEG C.
Under aseptic condition, with inoculation device inoculation, inoculum size is every bottle of 15g-20g.
After inoculation, culture bottle moves to and cultivates in room, and cultivation room requires dark, and Temperature Setting is at 20 DEG C-22 DEG C, and relative air humidity is between 60%-75%.Cultivate through 23d-25d, mycelia is covered with bottle.Mycelia is covered with rear 7d, enters and urges the flower bud phase, and urging Lei Wendu is 12 DEG C-16 DEG C, and relative air humidity is 90%-95%; While educating mushroom, temperature is controlled at 15 DEG C-18 DEG C in earlier stage, and latter temperature is controlled at 13 DEG C-16 DEG C, and humidity is controlled at 88%-92%.The Pleurotus eryngii that utilizes the substratum cultivation in the present embodiment, fruiting is neat, growth cycle is short, sporophore proterties is good, and output is significantly high than reference examples.
Embodiment 2: in the present embodiment, substratum comprises the dry-matter that water and water mix, the water content of this substratum is 63-66%, and dry-matter component and proportioning are as follows: Testa Viciae fabae 40%, wood chip 25%, wheat bran 16%, Semen Maydis powder 8%, rice bran 5%, dregs of beans 3%, lime powder 1%, gypsum 1.2%, calcium superphosphate 0.4%, potassium primary phosphate 0.4%.Preparation method's reference example 1.The Pleurotus eryngii that utilizes the substratum cultivation in the present embodiment, fruiting is neat, growth cycle is short, sporophore proterties is good, and output is significantly high than reference examples.
Embodiment 3: in the present embodiment, substratum comprises the dry-matter that water and water mix, the water content of this substratum is 63-66%, and dry-matter component and proportioning are as follows: Testa Viciae fabae 35%, wood chip 30%, wheat bran 18%, Semen Maydis powder 6%, rice bran 6%, dregs of beans 2%, lime powder 1%, gypsum 0.8%, calcium superphosphate 0.6%, potassium primary phosphate 0.6%.Preparation method's reference example 1.The Pleurotus eryngii that utilizes the substratum cultivation in the present embodiment, fruiting is neat, growth cycle is short, sporophore proterties is good, and output is significantly high than reference examples.
Control Example 4: in the present embodiment, substratum comprises the dry-matter that water and water mix, the water content of this substratum is 63-66%, and dry-matter component and proportioning are as follows: wood chip 65%, wheat bran 16%, Semen Maydis powder 8%, rice bran 5%, dregs of beans 3%, lime powder 1%, gypsum 1%, calcium superphosphate 0.5%, potassium primary phosphate 0.5%.
Table one Example formulations
Unit: % (quality)
The character pair ratios such as table two different ingredients Pleurotus eryngii mycelia growing way
Note: mycelial density with ++, +++ and ++++represent ,+number more multilist shows that mycelial density is higher
Table three different ingredients Pleurotus eryngii sporophore character pair ratio
Size and English alphabet represent the significance of difference of p < 0.01 or p < 0.05
Table one is four kinds of different ingredients.
Table two is four kinds of different ingredients Pleurotus eryngii mycelia growing way contrast tables, and the present invention fills a prescription 1,2,3 at mycelial density, the healthy and strong degree of mycelia, and mycelial growth rate, is all better than prior art formula 4, has shortened the production cycle simultaneously, has reduced cost.
Table three is four kinds of formula Pleurotus eryngii sporophore proterties contrast tables, and invention formula 1,2,3, at the aspect such as quality, output of Pleurotus eryngii, is all better than prior art formula 4.
As can be seen from the above, in Testa Viciae fabae culture material, add the culture material with nutrition and complementary structure, regulated the elasticity of substratum, make planting material both keep good air permeability, strengthened again water-absorbing-retaining ability.These good characteristics have ensured the high-yield high-quality of Pleurotus eryngii, and the utilization that simultaneously substitutes material Testa Viciae fabae has not only been saved raw materials cost but also improved biological efficiency, has great economic benefit and ecological benefits.
Claims (7)
1. a Pleurotus eryngii substratum, is characterized in that this substratum application Testa Viciae fabae is as matrix, and the dry-matter that comprises water and mix with water, and the water content of this substratum is 63-66%, and aforesaid dry-matter and mass percentage content thereof are as follows:
2. Pleurotus eryngii substratum according to claim 1, is characterized in that the particle diameter of described Testa Viciae fabae is below 5mm.
3. according to the preparation method of the Pleurotus eryngii substratum described in claim 1, it is characterized in that comprising following processing step:
1. take in proportion each component;
2. Testa Viciae fabae is mixed with wood chip, prewet with liming, the composting 24h that prewets, makes the water content of mixture remain on 55%-60%;
8. wheat bran, Semen Maydis powder, rice bran, dregs of beans siccative are poured in stirrer, stirred, add step mixture 2. and stir again;
4. by gypsum, calcium superphosphate, potassium primary phosphate is added to the water dissolving, and the aqueous solution is added in said mixture, fully stirs 15-20min, makes the water content of substratum remain on 63%-66%;
5. bottle, beat and plant bacterium hole, sterilizing, cooling.
4. preparation method according to claim 3, is characterized in that during step 2., the mass percent concentration of lime aqueous solution is 1%.
5. preparation method according to claim 3, is characterized in that during step 5. that when bottling stamps and plants bacterium hole, planting aperture, bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band.
6. preparation method according to claim 3, is characterized in that: described step 5. in sterilizing and cooling conditions as follows: first keep 100 DEG C of sterilizing 1h, then rise to 121 DEG C of autoclaving 1.5h, after sterilizing, be cooled to below 25 DEG C.
7. Pleurotus eryngii substratum according to claim 1, is characterized in that, after inoculation, and 20 DEG C-22 DEG C of culturing room's temperature, relative humidity 60%-75%, incubation time is 23d-25d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310069481.7A CN103102203B (en) | 2013-03-04 | 2013-03-04 | Pleurotus eryngii culture medium as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310069481.7A CN103102203B (en) | 2013-03-04 | 2013-03-04 | Pleurotus eryngii culture medium as well as preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103102203A CN103102203A (en) | 2013-05-15 |
| CN103102203B true CN103102203B (en) | 2014-08-13 |
Family
ID=48310442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310069481.7A Expired - Fee Related CN103102203B (en) | 2013-03-04 | 2013-03-04 | Pleurotus eryngii culture medium as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103102203B (en) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103299830A (en) * | 2013-06-10 | 2013-09-18 | 邬金飞 | Preparation method of edible fungus kernel (blended with rice) culture |
| CN103333023B (en) * | 2013-07-05 | 2014-12-24 | 邬金飞 | Pleurotus eryngii cultivation material compatibility and manufacture method of cultivation material |
| CN103333024B (en) * | 2013-07-18 | 2015-06-10 | 司天戎 | Facilitating pleurotus eryngii culture medium and preparation method thereof |
| CN103332992B (en) * | 2013-07-18 | 2015-06-10 | 司天戎 | High-quality pleurotus eryngii culture medium and preparation method |
| CN103408369B (en) * | 2013-08-08 | 2014-12-24 | 邬金飞 | Compatibility and manufacturing method of Pleurotus eryngii cultivation material |
| CN103420733A (en) * | 2013-08-22 | 2013-12-04 | 邬金飞 | Medicine compatibility and manufacturing method of abalone mushroom cultivation material |
| CN103420734B (en) * | 2013-08-22 | 2015-02-25 | 邬金飞 | Medicine compatibility and manufacturing method of straw mushroom cultivation material |
| CN103724113B (en) * | 2013-12-12 | 2016-06-01 | 凤台县千秋食用菌有限公司 | A kind of Enoki mushroom cultivation material containing Testa Viciae fabae and its preparation method |
| CN104119133B (en) * | 2014-06-18 | 2016-04-06 | 东至县兴农菌业有限责任公司 | A kind of maize straw is Pleurotus eryngii substratum of raw material and preparation method thereof |
| CN104261997A (en) * | 2014-09-25 | 2015-01-07 | 安徽天都灵芝制品公司 | High-yield pleurotus eryngii culture medium and preparation method thereof |
| CN104311250A (en) * | 2014-09-28 | 2015-01-28 | 铜陵市香江食用菌种植有限责任公司 | Pleurotus eryngii culture medium containing grassiness and preparation method thereof |
| CN104396561A (en) * | 2014-10-21 | 2015-03-11 | 云南畅佳农业科技有限公司 | Artificial cultivation method for Xerula pudens (Pers. :Fr.) sing |
| CN106069197A (en) * | 2016-07-01 | 2016-11-09 | 刘刚 | A kind of Lentinus Edodes soilless culture method |
| CN107619317A (en) * | 2017-09-15 | 2018-01-23 | 海门市利欣农产品有限公司 | A kind of pleurotus eryngii culture medium and preparation method thereof |
| CN108782015A (en) * | 2018-06-25 | 2018-11-13 | 佛山市三水区嘉信农业技术研究院（普通合伙） | It is a kind of to prepare collybia radicata culture medium matter and method using crop by-products |
| CN110089346A (en) * | 2019-05-05 | 2019-08-06 | 宁夏玛莎食品有限公司 | A kind of fructus lycii branch is the mushroom culture medium and preparation method thereof of raw material |
| CN110495348A (en) * | 2019-07-11 | 2019-11-26 | 永富饶（天津）农业科技发展有限公司 | A kind of preparation method of planting almond abalone mushroom material |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006212025A (en) * | 2005-01-07 | 2006-08-17 | Amikku Group:Kk | Dried powdered food |
| CN101759487A (en) * | 2008-11-19 | 2010-06-30 | 赵伟 | High-yield cultivation method of pleurotus eryngii |
| CN102807418A (en) * | 2011-05-31 | 2012-12-05 | 王来战 | Preparation method for pleurotus nebrodensis cultivation material |
| CN102845219B (en) * | 2012-09-06 | 2013-11-06 | 西北农林科技大学 | Cultivation technique of Pleurotus eryngii |
-
2013
- 2013-03-04 CN CN201310069481.7A patent/CN103102203B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN103102203A (en) | 2013-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103102203B (en) | Pleurotus eryngii culture medium as well as preparation method and application thereof | |
| CN101684053B (en) | Needle mushroom culture medium and preparation method | |
| CN103130572A (en) | Pleurotus geesteranus cultivation material and preparation method thereof | |
| CN103641584A (en) | An organic fertilizer and a preparation method thereof | |
| CN103102189B (en) | Organic fertilizer formula making use of factory sludge and seafood mushroom dregs and production process of organic fertilizer formula | |
| CN103864513A (en) | Compound microbial fertilizer and preparation method thereof | |
| CN103242092A (en) | Needle mushroom cultivation material taking tea pruning branches and leaves as main raw materials and manufacturing method | |
| CN104876691A (en) | Compost for black fungus cultivation and preparation method of compost | |
| CN103641556B (en) | Method for manufacturing pholiota nameko culture material from sunflower byproducts | |
| CN102442863A (en) | Formula of culture medium for yellow needle mushroom and preparation method of same | |
| CN103082145A (en) | Method for producing grape skin residue pig feed by utilizing lentinula edodes and yeast for symbiotic fermentation | |
| CN106396817A (en) | Method for preparing organic fertilizer based on straw and livestock and poultry manure | |
| CN103641587A (en) | An improved novel fertilizer and a preparation method thereof | |
| CN104402616A (en) | Oyster mushroom cultivation medium with use of garlic straw and oyster mushroom production method | |
| CN104030841A (en) | Edible mushroom cultivation material prepared by utilization of waste mushroom residues and preparation method thereof | |
| CN102823725A (en) | Method for producing biologic protein feed by using solid-state fermentation fiber dreg | |
| CN103910583B (en) | Culture medium of edible fungus utilizing palm byproduct to make and preparation method thereof | |
| CN104311231A (en) | Culture base-material for cultivating ganoderma lucidum from straw and biogas slag and preparation method thereof | |
| CN107500825A (en) | A kind of Se-enriched bio fertilizer and its preparation and application | |
| CN102898195A (en) | Method for preparing biological fertilizers from corn soaking liquid | |
| CN102674934B (en) | Water-retaining amino acid bio-organic fertilizer prepared from water hyacinth | |
| CN107032854A (en) | The method that stalk fermentation biogas residue prepares organic fertilizer | |
| CN107382592A (en) | A kind of white fungus matrix and preparation method thereof | |
| CN104326800A (en) | Method for preparing lentinula edodes culture medium with pine bark | |
| CN107162679A (en) | The cultural method of grifola frondosus cultivation matrix and preparation method thereof and grifola frondosus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wu Jinfei Inventor after: Wu Guiyang Inventor before: Wu Jinfei |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: WU JINFEI TO: WU JINFEI WU GUIYANG |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140813 Termination date: 20150304 |
|
| EXPY | Termination of patent right or utility model |