CN1669436A - Method for preparing plant growth regulator utilizing sea weed - Google Patents
Method for preparing plant growth regulator utilizing sea weed Download PDFInfo
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- CN1669436A CN1669436A CN 200410026546 CN200410026546A CN1669436A CN 1669436 A CN1669436 A CN 1669436A CN 200410026546 CN200410026546 CN 200410026546 CN 200410026546 A CN200410026546 A CN 200410026546A CN 1669436 A CN1669436 A CN 1669436A
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- plant growth
- growth regulator
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- marine alga
- seaweed
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Abstract
The invention discloses a kind of method that adopting seaweed to make plant growth regulator. It comprises the following steps: watering as the proportion of the dry seaweed and the water is 1,10 to 1,20, immersing and crutching the plasm, adding pectic enzyme, aquathermolysising, adjusting the pH with acid, edulcorating by filtration or centrifugation, gaining the upper clean fluid, and adjusting the pH with alkali, then we can get the plant growth regulator. The invention adopts new enzyme engineering production technique, screens fresh and proper seaweed breed, adopts mechanical method, doesn't sustain a temperature over 45 centigrade degree, is without any desiccation and refrigeration, just adopts the enzymolysis method to delivery the inner contents after the cell wall is broken, so it retains the seaweeds' natural active ingredient maximumly, and contains nutrition element, biology-resistant material and phytohormone. The new technology simplifies the production flow, improves the product quality and reduces the manufacturing expense.
Description
Technical field
The present invention relates to a kind of method of making plant growth regulator, particularly relate to a kind of enzyme engineering method that adopts and utilize marine alga to make the method for plant growth regulator.
Background technology
Plant growth promoter such as heteroauxin (IAA), indolebutyric acid (IBA), methyl (NAA), naphthoxyacetic acid (NOA), 2,4-D, drop-proof agent, production increasing agent, gibberellin etc. are the synthetic plant hormone, widely use on agricultural.But the pollution problem of using the synthetic plant hormone to produce has become very severe social concern, and China's people's health in serious harm.Working out efficient, the long agent of pollution-free green plant growth-promoting, is the needs of green agriculture revolution, also is the only way which must be passed that solves China's pollution of dining table.At present, traditional marine alga extraction process is to extract and the high temperature concentrate drying with akaline liquid, thereby has destroyed the active skull cap components of marine alga.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing marine alga to make plant growth regulator, this method can be produced environment-friendly type, free of contamination plant growth regulator.
For achieving the above object, technical solution of the present invention is:
The present invention is a kind of method of utilizing marine alga to make plant growth regulator, and it may further comprise the steps: (1) is 1 in the ratio of dry seaweed and water: the ratio of 10-20 adds water, soaks after 1-4 hour, smashes slurry; (2) ratio in dry seaweed and pectase is 1: the ratio of 0.0005-0.01 adds pectase, under 40-45 ℃ of temperature heating hydrolysis 2-4 hour; (3) being adjusted to pH value with acid is 2-3, places 2-4 hour, filters or the centrifugal impurity of removing, and gets supernatant; (4) being adjusted to pH value with alkali is 5-6, makes plant growth regulator.
Acid described in the step (3) is hydrochloric acid, acetic acid, citric acid, sulfuric acid.
Alkali described in the step (4) is sodium hydroxide, potassium hydroxide.
After adopting such scheme, because the key technology of invention is the active component that keeps marine alga natural as much as possible, the new technology that is adopted is: adopt enzyme engineering new technology production technology, the suitable fresh marine alga kind of screening, method with machinery, do not stand to surpass 45 ℃ high temperature, without any dehydration and freezing, just the method by enzymolysis discharges the broken content of cell wall, thereby the active component that has kept marine alga natural greatly, the collection nutrient component, antibiotics, plant hormone is in one, not only can improve seed germination rate in a large number, increase the output of crops and improve the quality of crops, and can improve soil property, promote the absorption of plant to nutriment in the soil, strengthen the immunity of crops, reduce fungi, insect and nematode be to the infringement of crops, thereby more can resist extraneous poor environment (drought, waterlogging) variation.And the present invention adopts enzymatic hydrolysis and acid-treated new technology, has simplified production procedure, improves product quality, reduces producing cost.
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment
Embodiment 1:
Get dried sea-tangle 100 and add water 1000L and soak after 2 hours, smash slurry, add 0.4 pectase, 45 ℃ of water-bath heating hydrolysis 4 hours, being adjusted to pH value with 6mol/L hydrochloric acid is 2, places 4 hours.Get supernatant, remove by filter impurity with nylon cloth, regulating pH value with 6mol/L sodium hydroxide is 5, makes plant growth regulator.In use, with 50 times of this plant growth regulator dilutions.
Embodiment 2:
Get dried sea-tangle 100 and add water 2000L and soak after 3 hours, smash slurry, add 0.8 pectase, 45 ℃ of water-bath heating hydrolysis 2 hours, being adjusted to pH value with 6mol/L hydrochloric acid is 2, places 2 hours.Get supernatant, remove by filter impurity with nylon cloth, regulating pH value with 6mol/L sodium hydroxide is 5, makes plant growth regulator.In use, with 50 times of this plant growth regulator dilutions.
Embodiment 3:
Get dried sea-tangle 100 and add water 1500L and soak after 4 hours, smash slurry, add 0.6 pectase, 40 ℃ of water-bath heating hydrolysis 3 hours, being adjusted to pH value with 6mol/L hydrochloric acid is 3, places 3 hours.Get supernatant, remove by filter impurity with nylon cloth, regulating pH value with 6mol/L sodium hydroxide is 6, makes plant growth regulator.In use, with 50 times of this plant growth regulator dilutions.
Embodiment 4:
Get dried sea-tangle 100 and add water 1000L and soak after 4 hours, smash slurry, add 0.6 pectase, 45 ℃ of water-bath heating hydrolysis 3 hours, being adjusted to pH value with 6mol/L acetic acid is 3, places 3 hours.Get supernatant, remove by filter impurity with nylon cloth, regulating pH value with 6mol/L potassium hydroxide is 6, makes plant growth regulator.In use, with 50 times of this plant growth regulator dilutions.
If adopt fresh marine alga, then the ratio in fresh marine alga and water is 1: the ratio of 5-8 adds water, and be 1 in the ratio of fresh marine alga and pectase: the ratio of 0.0005-0.008 adds pectase.
Embodiment 5:
Get fresh marine alga 100 and add water 500L and soak after 1 hour, smash slurry, add 0.4 pectase, 45 ℃ of water-bath heating hydrolysis 3 hours are 2 with 6mol/L hydrochloric acid adjusting pH value, place 3 hours.Get supernatant, remove by filter impurity with nylon cloth, regulating pH value with 6mol/L sodium hydroxide is 5, makes plant growth regulator.In use, with 100 times of this plant growth regulator dilutions.
The present invention adopts the enzyme engineering method, from marine alga, extract active ingredient, make the concentrated liquid product, be the pure natural fruit and vegetable growth promoter, the foliage spray of fruit offerings vegetables crop is used, this fruits and vegetables growth promoter is green, complete environmentally friendly machine, show that through field experiment product can significantly improve fruit-vegetable plant output, and have certain anthelmintic action, being the strong assistant who produces green agricultural product, also is one of effective way of eliminating pollution of dining table.
Fruits and vegetables growth promoter " Hai Zhibao " product produced according to the invention has carried out land for growing field crops, booth application test.
Result of the test 1:
1, logical heart dish (the logical heart dish of cock mark green bamboo snake bone) booth foliage spray.Adopt " Hai Zhibao " to use under 200-400 times of concentration of dilution for No. 1, No. 2, No. 3, one heart stage of three leaves, begin to spray, and used once in per 10 days.Result of the test shows that the experimental group comparison is good according to group leader's gesture, the blade face is thick, output balanced growth 11.1%.
2, grow eggplant (Taiwan 703 long eggplants) land for growing field crops application test.Just the fruit phase begins to spray, and method is the same.The result shows: the experimental group comparison is good according to group leader's gesture, fruiting rate is high.Average volume increase 38.7%.
The result shows that " Hai Zhibao " that the present invention produces is pure natural, pollution-free product, is suitable for producing the green fruit-vegetable product.
Result of the test 2:
1, grows eggplant and plucked,, receive altogether three times to December 17 since November 28.The crop statistics sees Table.As can be seen from the table, No. 1, the effect difference of No. 2 and No. 3 " Hai Zhibao " increase by 46%, 43% and 23% than control group respectively.The vegetable grower is reflected three medication group leader eggplant smooth surfaces, and commodity value is higher, helps selling.Medication group plant two spotted spider mite and mite class obviously reduce, and illustrate that " Hai Zhibao " has anthelminthic effect to a certain degree.
Long eggplant output statistics table
| Project | Control group | No. 1 | No. 2 | No. 3 |
| The output (jin) of gathering for the first time | ???3.1 | ????5 | ????4.5 | ??????3.4 |
| The output (jin) of gathering for the second time | ???9.1 | ????10 | ????11.7 | ??????10.4 |
| The output of gathering for the third time (jin) | ???5.1 | ????10.3 | ????8.5 | ??????7.4 |
| Add up to output (jin) | ???17.3 | ????25.3 | ????24.7 | ??????21.2 |
| Volume increase (jin) | ???/ | ????8 | ????7.4 | ??????3.9 |
| Percentage increases (%) | ???/ | ????46 | ????43 | ??????23 |
From the monoblock growing area, the bacterial wilt of medication group is low weight.But the effect that whether has anti-eggplant bacterial wilt also need continue to observe, test.
2, the capsicum phase of bearing fruit does not also gather in the crops, field observation setting situation, and No. 1 is better than control group, but effect is general; No. 2 than control group difference; No. 3 are obvious better than control group, the percentage of fertile fruit height, and pepper fruit is bigger.
Claims (3)
1, a kind of method of utilizing marine alga to make plant growth regulator, it is characterized in that: it may further comprise the steps: (1) is 1 in the ratio of dry seaweed and water: the ratio of 10-20 adds water, soaks after 1-4 hour, smashes slurry; (2) ratio in marine alga and pectase is 1: the ratio of 0.0005-0.01 adds pectase, under 40-45 ℃ of temperature heating hydrolysis 2-4 hour; (3) being adjusted to pH value with acid is 2-3, places 2-4 hour, filters or the centrifugal impurity of removing, and gets supernatant; (4) being adjusted to pH value with alkali is 5-6, makes plant growth regulator.
2, the method for utilizing marine alga to make plant growth regulator according to claim 1, it is characterized in that: the acid described in the step (3) is hydrochloric acid, acetic acid, citric acid, sulfuric acid.
3, the method for utilizing marine alga to make plant growth regulator according to claim 1, it is characterized in that: the alkali described in the step (4) is sodium hydroxide, potassium hydroxide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410026546 CN1669436A (en) | 2004-03-16 | 2004-03-16 | Method for preparing plant growth regulator utilizing sea weed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410026546 CN1669436A (en) | 2004-03-16 | 2004-03-16 | Method for preparing plant growth regulator utilizing sea weed |
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| Publication Number | Publication Date |
|---|---|
| CN1669436A true CN1669436A (en) | 2005-09-21 |
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| CN 200410026546 Pending CN1669436A (en) | 2004-03-16 | 2004-03-16 | Method for preparing plant growth regulator utilizing sea weed |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104245899A (en) * | 2011-10-05 | 2014-12-24 | Sea6能源有限公司 | Process of production of renewable chemicals and biofuels from seaweeds |
| CN105325466A (en) * | 2015-12-10 | 2016-02-17 | 沈阳化工研究院有限公司 | Composition for inducing plants to resist diseases and adjusting plant growth and preparation method and application of composition |
| CN107183067A (en) * | 2017-06-08 | 2017-09-22 | 中国水产科学研究院黄海水产研究所 | Method for preparing seaweed plant growth regulator by integral biological enzymolysis method |
| CN107846905A (en) * | 2015-05-10 | 2018-03-27 | Sea6能源私人有限公司 | For improving the biostimulation agent formulation and its purposes of plant growth |
-
2004
- 2004-03-16 CN CN 200410026546 patent/CN1669436A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104245899A (en) * | 2011-10-05 | 2014-12-24 | Sea6能源有限公司 | Process of production of renewable chemicals and biofuels from seaweeds |
| CN104245899B (en) * | 2011-10-05 | 2016-10-19 | Sea6能源有限公司 | The method being produced reproducible chemicals and bio-fuel by Sargassum |
| CN107846905A (en) * | 2015-05-10 | 2018-03-27 | Sea6能源私人有限公司 | For improving the biostimulation agent formulation and its purposes of plant growth |
| CN107846905B (en) * | 2015-05-10 | 2020-10-23 | Sea6能源私人有限公司 | Biostimulant formulations for improved plant growth and uses thereof |
| CN105325466A (en) * | 2015-12-10 | 2016-02-17 | 沈阳化工研究院有限公司 | Composition for inducing plants to resist diseases and adjusting plant growth and preparation method and application of composition |
| CN107183067A (en) * | 2017-06-08 | 2017-09-22 | 中国水产科学研究院黄海水产研究所 | Method for preparing seaweed plant growth regulator by integral biological enzymolysis method |
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