CN1800345A - Culture medium for pleurotus ferulae invigoration - Google Patents
Culture medium for pleurotus ferulae invigoration Download PDFInfo
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- CN1800345A CN1800345A CN 200510130989 CN200510130989A CN1800345A CN 1800345 A CN1800345 A CN 1800345A CN 200510130989 CN200510130989 CN 200510130989 CN 200510130989 A CN200510130989 A CN 200510130989A CN 1800345 A CN1800345 A CN 1800345A
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- asafoetide
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Abstract
The invention relates to an asafetida mushroom customs agent who adds asafetida or asafetida hay meal or asafetida hay abstraction into the normal mother seed customs agent or cultivating seed customs agent to obtain the customs agent which can restore the asafetida.
Description
Technical field
The present invention relates to a kind of Resina Ferulae mushroom rejuvenation substratum and preparation method thereof, solve in the Resina Ferulae mushroom suitability for industrialized production problem that bacterial classification is degenerated easily.
Background technology
Resina Ferulae mushroom also claims Pleurotus nebrodensis, white glossy ganoderma mushroom, asafoetida mushroom etc., its formal name used at school Pleurotus ferulae Lanzi (Pleurotus ferulae).Gain the name at a kind of medicinal plant asafoetide because of it is parasitic or saprophytic.Its delicious flavour has various health care functions, is the edible mushrooms that has very much exploitation to be worth.1970, the Frenchman was successful with straw, useless oat domesticating and cultivating numb and 20%.Nineteen eighty-three, China scientific worker begins the Xinjiang Wild Resina Ferulae mushroom is carried out the domesticating and cultivating test, adopts weed tree sawdust, cotton seed hulls and the success of wheat bran domesticating and cultivating.
The Resina Ferulae mushroom bacterial classification is after artificial many generation breedings, and the shape of Resina Ferulae mushroom, mouthfeel, nutritive value all degradation phenomena can occur, and resistance against diseases also will weaken, its commodity value will reduce greatly, therefore, in suitability for industrialized production, be necessary often the Resina Ferulae mushroom bacterial classification to be carried out rejuvenation, avoid degenerating.At present, in the suitability for industrialized production of Resina Ferulae mushroom, people generally adopt potato culture to come the rejuvenation bacterial classification, but through the bacterial classification of potato culture rejuvenation through 2-3 be commissioned to train support after, bacterial classification has degeneration again, rejuvenation again, and this influences the Resina Ferulae mushroom suitability for industrialized production greatly.Therefore being necessary to seek a kind of new rejuvenation substratum replaces existing potato culture.
The present invention is by adding asafoetide grass dry powder or food of the gods or asafoetide grass extract in traditional potato culture prescription, through 5-6 be commissioned to train support after, do not find that still the Resina Ferulae mushroom bacterial classification has degradation phenomena, it is minimum in aspect difference such as shape, mouthfeel and nutritive values to cultivate the bright Resina Ferulae mushroom and the wild Resina Ferulae mushroom that grow through the present invention, have characteristics such as growth cycle weak point, output height simultaneously, thereby created favourable condition for the suitability for industrialized production of Resina Ferulae mushroom.
Summary of the invention
The object of the invention is, the Resina Ferulae mushroom rejuvenation substratum of development is to add the extract of food of the gods or asafoetide grass dry powder or asafoetide grass in traditional mother culture media or pedigree seed culture medium or the cultivar substratum, obtain to make the substratum of asafoetide rejuvenation, Resina Ferulae mushroom is female plants through after the substratum rejuvenation provided by the invention, has the weak point in vegetative period, and fruiting is fast, individual big, the good characteristic that output is high can significantly increase Resina Ferulae mushroom output, solves the problem that the Resina Ferulae mushroom bacterial classification is degenerated aborning easily.
A kind of Resina Ferulae mushroom rejuvenation substratum of the present invention is to add the extract of food of the gods or asafoetide grass dry powder or asafoetide grass in mother culture media or the cultivar substratum, obtains the substratum of asafoetide rejuvenation;
Each set of dispense ratio of mother culture media is:
1000 milliliters of potato liquors, glucose 20 grams, the food of the gods consumption is that the weight ratio 0.01-10% of substratum total amount is optional, or the weight ratio 1-50% that the careless dry powder consumption of asafoetide is the substratum total amount is optional, or the weight ratio 0.1-20% that the careless extract consumption of asafoetide is the substratum total amount is optional, agar 20 grams, pH value 5.5-6.0;
Each set of dispense ratio of pedigree seed culture medium is:
Wheat adds the water boiling to there not being the white heart, plant the skin crack-free, pick up and dry, add terra alba 6%, lime carbonate 1%, the food of the gods consumption is that the weight ratio 0.01--10% of substratum total amount is optional, or the weight ratio 1-50% that the careless dry powder consumption of asafoetide is the substratum total amount is optional, or the weight ratio 0.1-20% that the careless extract consumption of asafoetide is the substratum total amount is optional;
Each set of dispense ratio of cultivar substratum is:
Cotton seed hulls 78%, wheat bran 20%, sucrose 1%, gypsum 1%, the food of the gods consumption is that the weight ratio 0.01-10% of substratum total amount is optional, or the weight ratio 1-50% that the careless dry powder consumption of asafoetide is the substratum total amount is optional, or the weight ratio 0.1-20% that the careless extract consumption of asafoetide is the substratum total amount is optional.
Food of the gods is the resin that extracts on Ferula sinkiangensisK.M.Shen or the Ferula fukanensis K.M.Shen.
The asafoetide grass is Ferula sinkiangensisK.M.Shen or Ferula fukanensis K.M.Shen over-ground part.
Asafoetide grass extract be with asafoetide grass water or methyl alcohol or ethanol or chloroform or acetone or ethyl acetate through soaking or the solution of thermal backflow extraction or ultrasonic extraction, the medicinal extract that concentrate drying obtains wherein contains the composition of asafoetide polyose.
The preparation method of Resina Ferulae mushroom rejuvenation substratum follows these steps to carry out:
Preparation mother culture media: earlier fresh disease-free potato is cleaned, be cut into small thin slices after the peeling, add 1000 milliliters in water, boil 20 minutes after-filtration, its filtered juice is the potato liquor, it is candy molten to add agar and sucrose or grape then, after adding the extract or asafoetide grass dry powder of food of the gods or asafoetide grass, supply moisture to 1000 milliliter, after double gauze filters, the sheet glass ware of packing into sterilization obtains Resina Ferulae mushroom rejuvenation substratum;
The preparation pedigree seed culture medium:
Wheat adds the water boiling to there not being the white heart, plant the skin crack-free, pick up and dry, add terra alba 6%, lime carbonate 1% is behind the extract of adding food of the gods or asafoetide grass or the asafoetide grass dry powder, supply moisture to 1000 milliliter, after double gauze filtered, the sheet glass ware of packing into sterilization obtained Resina Ferulae mushroom rejuvenation substratum;
Preparation cultivar substratum:
Take by weighing cotton seed hulls 78%, wheat bran 20%, sucrose 1%, gypsum 1% is cooled to room temperature behind high-temperature sterilization, behind the extract of adding food of the gods or asafoetide grass or the asafoetide grass dry powder, supply moisture to 1000 milliliter, after double gauze filtered, the sheet glass ware of packing into sterilization obtained Resina Ferulae mushroom rejuvenation substratum;
A kind of Resina Ferulae mushroom rejuvenation substratum of the present invention, through with the contrast substratum test its results are shown in Table:
Table 1 is respectively organized Resina Ferulae mushroom mycelial growth velocity ratio
| Group | n | Average daily long speed (mm/ days) | The dense degree of mycelia |
| Potato culture positive control (magnesium sulfate) Pleurotus ferulae rejuvenation culture medium 1 (gum asafetida) Pleurotus ferulae rejuvenation culture medium 2 (asafoetide grass extract) Pleurotus ferulae rejuvenation culture medium 3 (asafoetide grass) | 10 10 10 10 10 | 4.38 4.45 5.58* 5.53* 5.46* | ++ ++++ +++ +++ |
Compare * * P＜0.01 with control group; + represent dense degree
As can be seen from Table 1, after the female rejuvenation substratum rejuvenation cultivation of planting of Resina Ferulae mushroom through the present invention's development, significantly be better than existing potato rejuvenation substratum at aspects such as the speed of growth, the dense degree of mycelia, by the pilot plant test to mother's kind, original seed, cultivar rejuvenation nutrient chemical, its result shows: the Resina Ferulae mushroom rejuvenation substratum of the present invention's development can be competent at suitability for industrialized production fully.
Embodiment
The preparation method of a kind of Resina Ferulae mushroom rejuvenation substratum of the present invention follows these steps to carry out:
Resina Ferulae mushroom mother culture media: earlier fresh disease-free potato is cleaned, be cut into small thin slices after the peeling, claim 200 grams, add 1000 milliliters in water, boil 20 minutes after-filtration, its filtered juice is the potato liquor, add then agar 20 gram and glucose 20 grams boil molten, add the extract or the careless dry powder of asafoetide of food of the gods or asafoetide grass after, supply moisture to 1000 milliliter, after double gauze filtered, the sheet glass ware of packing into sterilization obtained Resina Ferulae mushroom rejuvenation substratum.Specific embodiment sees Table 2.
Table 2 adds the ratio of the extract or the asafoetide grass dry powder of food of the gods or asafoetide grass for mother culture media
| Sequence number | Food of the gods % | Or asafoetide grass dry powder % | Or asafoetide grass extract % |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 | 0.01 0.05 0.10 0.15 0.40 0.60 0.80 1.0 2.5 4.0 6.5 8.0 10.0 | 1 5 8 10 15 18 20 25 30 35 40 45 50 | 0.1 0.3 0.7 1.0 3.5 5.0 7.5 8.0 9.0 10 15 18 20 |
Resina Ferulae mushroom pedigree seed culture medium: wheat is added the water boiling to there not being the white heart, plant the skin crack-free, pick up and dry, add terra alba 6%, lime carbonate 1% is behind the extract of adding food of the gods or asafoetide grass or the asafoetide grass dry powder, supply moisture to 1000 milliliter, after double gauze filtered, the sheet glass ware of packing into sterilization obtained Resina Ferulae mushroom rejuvenation substratum.Specific embodiment sees Table 3.
Table 3 adds the ratio of the extract or the asafoetide grass dry powder of food of the gods or asafoetide grass for pedigree seed culture medium
| Sequence number | Food of the gods % | Or asafoetide grass dry powder % | Or asafoetide grass extract % |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 | 0.01 0.05 0.10 0.15 0.40 0.60 0.80 1.0 2.5 4.0 6.5 8.0 10.0 | 1 5 8 10 15 18 20 25 30 35 40 45 50 | 0.1 0.3 0.7 1.0 3.5 5.0 7.5 8.0 9.0 10 15 18 20 |
Resina Ferulae mushroom cultivar substratum: take by weighing cotton seed hulls 78%, wheat bran 20%, sucrose 1%, gypsum 1% is cooled to room temperature behind high-temperature sterilization, behind the extract of adding food of the gods or asafoetide grass or the asafoetide grass dry powder, supply moisture to 1000 milliliter, after double gauze filtered, the sheet glass ware of packing into sterilization obtained Resina Ferulae mushroom rejuvenation substratum.Specific embodiment sees Table 4.
Table 4 adds the ratio of the extract or the asafoetide grass dry powder of food of the gods or asafoetide grass for the cultivar substratum
| Sequence number | Food of the gods % | Or asafoetide grass dry powder % | Or asafoetide grass extract % |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 | 0.01 0.05 0.10 0.15 0.40 0.60 0.80 1.0 2.5 4.0 6.5 8.0 10.0 | 1 5 8 10 15 18 20 25 30 35 40 45 50 | 0.1 0.3 0.7 1.0 3.5 5.0 7.5 8.0 9.0 10 15 18 20 |
Claims (4)
1, a kind of Resina Ferulae mushroom rejuvenation substratum is characterized in that this substratum is the extract that adds food of the gods or asafoetide grass dry powder or asafoetide grass in mother culture media commonly used or pedigree seed culture medium or cultivar substratum, obtains the substratum of asafoetide rejuvenation, wherein:
Each set of dispense ratio of mother culture media is:
1000 milliliters of potato liquors, glucose 20 grams, the food of the gods consumption is that the weight ratio 0.01-10% of substratum total amount is optional, or the weight ratio 1-50% that the careless dry powder consumption of asafoetide is the substratum total amount is optional, or the weight ratio 0.1-20% that the careless extract consumption of asafoetide is the substratum total amount is optional, agar 20 grams, pH value 5.5-6.0;
Each set of dispense ratio of pedigree seed culture medium is:
Wheat adds the water boiling to there not being the white heart, plant the skin crack-free, pick up and dry, add terra alba 6%, lime carbonate 1%, the food of the gods consumption is the weight ratio 0.01 of substratum total amount---10% is optional, or the weight ratio 1-50% that the careless dry powder consumption of asafoetide is the substratum total amount is optional, or the weight ratio 0.1-20% that the careless extract consumption of asafoetide is the substratum total amount is optional;
Each set of dispense ratio of cultivar substratum is:
Cotton seed hulls 78%, wheat bran 20%, sucrose 1%, gypsum 1%, the food of the gods consumption is that the weight ratio 0.01-10% of substratum total amount is optional, or the weight ratio 1-50% that the careless dry powder consumption of asafoetide is the substratum total amount is optional, or the weight ratio 0.1-20% that the careless extract consumption of asafoetide is the substratum total amount is optional.
2, Resina Ferulae mushroom rejuvenation substratum according to claim 1 is characterized in that food of the gods is the resin that extracts on Ferula sinkiangensisK.M.Shen or the Ferula fukanensis K.M.Shen.
3, Resina Ferulae mushroom rejuvenation substratum according to claim 1 is characterized in that the asafoetide grass is Ferula sinkiangensisK.M.Shen or top, Ferula fukanensis K.M.Shen ground.
4, Resina Ferulae mushroom rejuvenation substratum according to claim 1, it is characterized in that asafoetide grass extract is with asafoetide grass water or methyl alcohol or ethanol or chloroform or acetone or the ethyl acetate process is soaked or the solution of thermal backflow extraction or ultrasonic extraction, the medicinal extract that concentrate drying obtains wherein contains the composition of asafoetide polyose.
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| CN100419067C CN100419067C (en) | 2008-09-17 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100455654C (en) * | 2006-07-24 | 2009-01-28 | 曾宪贤 | Heat tolerant asafetida mushroom mutant |
| CN104926506A (en) * | 2015-06-19 | 2015-09-23 | 桂林健成生物科技开发有限公司 | Application of seed coat/embryo body and rootstock after sprouting vegetable harvesting in pleurotus nebrodensis cultivation |
| CN115088735A (en) * | 2022-07-15 | 2022-09-23 | 石河子大学 | Application of pleurotus ferulae aqueous extract in preventing and treating plant diseases |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1249128A (en) * | 1998-09-28 | 2000-04-05 | 北京金信食用菌有限公司 | Technique for cultivating Bailing mushroom |
| CN1320364A (en) * | 2000-09-09 | 2001-11-07 | 贵州省小西南食用菌工贸有限责任公司 | Method for producing rare edible fungus rich in Zn and Se |
| CN1256014C (en) * | 2002-11-18 | 2006-05-17 | 薛兵 | Industrialized production method of bai-ling mushroom |
| CN1233316C (en) * | 2003-05-20 | 2005-12-28 | 王彦丁 | Asafetida extract and its pharmaceutical use |
| KR100543726B1 (en) * | 2003-11-26 | 2006-01-20 | 차월석 | Synergistic compostion of anticancer effect containing extract of Pleurotus ferulae |
| CN1305366C (en) * | 2004-05-14 | 2007-03-21 | 延淑洁 | Cultivation technique of Pleurtotus nebrodensis |
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2005
- 2005-12-15 CN CNB2005101309899A patent/CN100419067C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100455654C (en) * | 2006-07-24 | 2009-01-28 | 曾宪贤 | Heat tolerant asafetida mushroom mutant |
| CN104926506A (en) * | 2015-06-19 | 2015-09-23 | 桂林健成生物科技开发有限公司 | Application of seed coat/embryo body and rootstock after sprouting vegetable harvesting in pleurotus nebrodensis cultivation |
| CN115088735A (en) * | 2022-07-15 | 2022-09-23 | 石河子大学 | Application of pleurotus ferulae aqueous extract in preventing and treating plant diseases |
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| CN100419067C (en) | 2008-09-17 |
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