CN1320364A - Method for producing rare edible fungus rich in Zn and Se - Google Patents

Abstract

A method for culturing rare edible fungus rich in Zn and Se fuatures that the well known rare edible funguses including Stropharia, asafetida mushroom, etc are used as pure cultures and the inorganic Zn and Se elements are absorbed by their biosomes to increase their content to 300-500 ppm. Said edible funguses can improve human immunity and the activity of more enzymes, and has high effect on preventing heart disease, diabetes and liver disease.

Description

Be rich in zinc, the rare Edible Fungi method of selenium

The invention provides a kind of rare Edible Fungi method that is rich in the zinc trace element selenium.

At present domestic existing report only in Hericium erinaceus, Asparagus and mushroom are produced, adopt and on medium, add trace element and tame, the kind utilization scope of cultivation is narrower.

The present invention is mainly used in the rare edible fungus variety that present bacterium industry is generally acknowledged, as yellow big foot mushroom (spherical cap bacterium a kind of) flower mushroom, Pleuotus nebrodensis Quel (claiming Pleurotus ferulae Lanzi again) etc., this technology can all can be suitable for by tame edible mushroom existing simultaneously.

The rare edible mushroom that method provided by the invention is produced has not only kept outside the various amino acid nutrient compositions of original edible mushroom, also improve the content of selenium, zinc microelement greatly, selenium, zinc are the indispensable trace elements of human body, by improving edible mushroom, particularly the zinc of rare edible mushroom, Se content after human body is taken in, can improve the immune disease-resistance ability of function of human body, strengthen the activity of plurality of enzymes, cardiopathy, hepatopathy, diabetes, cancer etc. are had remarkable preventive effect.

The present invention absorbs by organism by utilizing conversion technology with inorganic trace element, improves the content of edible mushroom zinc, selenium.Simultaneously rare edible mushroom itself is rich in that human body self can not synthesize and essential 8 seed amino acids and other multiple amino acid to needed by human body, vitamin and mineral matter, people make natural pollution-free food by the rare edible thalline that is rich in selenium, zinc, so that the requirement of human body to various trace elements such as selenium, zinc and several amino acids to be provided.

Method provided by the invention mainly be applicable to Pleuotus nebrodensis Quel (Pleurotus ferulae Lanzi), yellow big foot mushroom, flower mushroom belong in the warm type kind, to the claimed range of temperature, humidity be: the mycelium cultivation stage is an optimum with 22-25 ℃, and cultivation humidity is 60-65%.The cultivate material water content is controlled at 70%, and the sporophore growth temperature is 8-25 ℃, and relative air humidity is controlled at 90-95%, 15-20 ℃ of optimum fruiting temperature.

Concrete cultivation method of the present invention:

1. at first be strain domestication

(1) the 300ppm gradient is adopted in mother culture media prescription and making.

Mother culture media selects for use solid material (as thin iblet, millet or jowar) to make medium, and its prescription is; Water 100 grams, composite fertilizer's 10 grams, potassium dihydrogen phosphate 8 grams, white sugar 10 grams are mixed with soak, adjusting pH value with pulverized limestone is that 7.5-8 leaves standstill and heavyly gets supernatant after clear, soak the thin iblet of 500 grams, general immersion drains to put in the steamer after 4 hours and cooks, taking-up spreading for cooling in the porcelain basin, and gently be twisted into shot, add natural zinc, the selenium humus soil of containing by 5%, press the 300ppm gradient and add 3-8 gram sodium selenite solution, 5-20 restrains solution of zinc sulfate, 18 * 180mm pack in vitro after mixing is mixed thoroughly, carry out tampon, put into pressure cooker and sterilized 50 minutes down with 1.2kg pressure.Plant pipe and be cooled to about 25 ℃, just can transfer in the sterilization scope needs the bacterial classification of domestication.Cultivated 20 hours in preference temperature, visible bacterial classification is sprouted.Should note observing the growth response situation of bacterium around here, just can cover with test tube in general about 10 days, get sturdy, pure white, dense, the neat kind pipe of mycelia and continue to employ.

(2) the 500ppm gradient is adopted in pedigree seed culture medium prescription and making.

The proportioning of soak is made: clear water 5000 grams, composite fertilizer's 50 grams, potassium dihydrogen phosphate 40 grams, white sugar 50 grams are mixed with soak, and adjusting the pH value with pulverized limestone is 10-12.Clarification is got supernatant and is soaked wheat 3000 grams, and soak time is according to season, and weather is respectively between 36-72 hour, till germinateing with thorough swelling.The wheat of swelling is drained the water, put boil in the pot to outside pull out when giving birth in ripe, stand-by with draining the water again after the saturating cold of cold water.The raw material proportioning: wheat 3000 grams, wood chip 300 grams, wheat bran or rice bran 600 grams, land plaster 60 grams, the humus soil 300 that contains zinc, selenium 10% restrains; Promptly press the 500ppm gradient and add sodium selenite solution 25-40 gram, the humic soil of solution of zinc sulfate 40-80 gram preparation.

Preparation method: earlier gypsum and rice bran are mixed thoroughly, got 2/3 and admix the wheat material; After 1/3 remaining and wood chip mixed thoroughly, with the water that boils wheat mix wet to not following of the globule between clenching of the hand spice webs be to spend, (water content is about 65-70%) gets 4/5 and admixes the wheat material, it is degree that remaining 1/5 water droplet of mixing wet alunite spice in one's hands and have between referring to line with the water that boils wheat again flows down, with this material lid charge level, with 750 milliliters of bottled material of bacterium, seal overcoat brown paper with big tampon, put in the pressure cooker and sterilized 150-180 minute, take out the mother's kind that inserts the 300ppm gradient after cooling, under thermophilic, cultivate 15-18 days mycelia and can cover with bottle.

(3) produce the batching of planting and adopt the 800ppm gradient with making.

Produce kind of a production process, materials and original seed are similar, have only charge ratio to adjust, the batching prescription: 20 kilograms of wheats, 4 kilograms of wood chips, 2.4 kilograms in rice bran, 0.54 kilogram of land plaster, 2.6 kilograms of humus soil, sodium selenite solution 200-800 gram, Adlerika 400-1000 gram.

The preparation of cultivate material: the 1200ppm gradient is adopted in cultivation

Cultivate material adds sodium selenite solution 5-40 gram by every kilogram, Adlerika 10-80 gram, and the cultivation materials all select to contain natural zinc, selenium straw, concrete cultivation method; With the 800ppm gradient produce kind adopt following manner all can: 1, whole careless field production; 2, pocket wall formula cultivation; 3, taking off a bag land for growing field crops plants.

Embodiment is as follows:

Produce yellow big foot mushroom in mother plants 300ppm gradient domestication, under 22-25 ℃ of temperature, switching kind of recovery is slower, and early stage material feeding mycelium germination is produced irregular, and long during to the bed of material 2cm left and right sides when mycelia, all can neatly grow seems pure white, vigorous.Separate kind of a recovery and sprout soon, visible mycelium was sprouted in general 18-20 hour, to this gradient formulation material is stronger than adaptability, growing way good.Original seed is produced and is planted in the domestication of 800ppm gradient at 500ppm, material feeding reaction normal condition.In the incubation, temperature all can normal growth 10-30 ℃ of mycelia.Cultivate material is pressed 1200ppm gradient proportioning, cultivate material adopts straw, forage, pulverizing, adds sodium selenite solution 20-30 gram by every kilogram, and Adlerika 40-60 restrains the pack that stirs, with the production kind inoculation of 800ppm gradient, adopt the cultivation of pocket wall formula pile.Cultivation under 6-32 ℃ of condition, the mycelia material feeding in order, the fruiting situation is 15-20 ℃ of the best.It is strong that mycelium transforms capacity of decomposition, and fruit body organic zinc, Se content can be up to 3000-500ppm.

Claims (6)

1. one kind is rich in the rare Edible Fungi method of zinc selenium, it is characterized in that female solid culture medium of planting, its raw material outfit (weight ratio) nitrogen-phosphorus-potassium compound fertilizer 10 grams, phosphoric acid dioxy potassium 8 grams, sucrose 10 grams, water 1000 grams are mixed with soak, the pH value is transferred to 7.5-8, soaking solid grain composts or fertilisers of cultivating cooks, spreading for cooling adds a certain amount of sodium selenite solution, solution of zinc sulfate, preparation contain zinc, selenium humus soil.

2. Edible Fungi method according to claim 1, the charge ratio of its original seed is: composite fertilizer's 50 grams, potassium dihydrogen phosphate 40 grams, sucrose 50 grams, water 5000 grams, the preparation soak, the pH value is set up 10-12, soaks batching: the humus soil of 3 kilograms of wheats, 0.3 kilogram of wood chip, wheat bran or 0.6 kilogram in rice bran, land plaster 60 grams, sodium selenite, zinc sulphate preparation.

3. Edible Fungi method according to claim 1, the preparing ingredients prescription that it is produced kind: 20 kilograms of wheats, 4 kilograms of wood chips, 2.4 kilograms in rice bran, 0.54 kilogram of land plaster, contain 2.6 kilograms of the humus soil of sodium selenite, zinc sulphate.

4. Edible Fungi method according to claim 1, its production cultivate material are selected for use and are contained selenium, the higher straw of zinc, press sodium selenite solution and 400-1000 gram solution of zinc sulfate that the per kilogram cultivate material adds the 200-800 gram.

5. according to claim 1,2,3,4, described cultivating method for edible fungi, it is characterized in that female 300ppm of kind, cultivation temperature 22-25 ℃, original seed 500ppm, produce kind of 10-30 ℃ of a 800ppm cultivation temperature, cultivate material press 1200ppm, cultivates that temperature is moisture 70% at 6-32 ℃, 15-20 ℃ of sporophore growth temperature, medium humidity 60-65%, cultivate material, relative air humidity 85-95%.

6. production method according to claim 5, autoclaved pressure were sterilized 50-180 minute under 1.2 kilograms of pressure.