CN115088735A - Application of pleurotus ferulae aqueous extract in preventing and treating plant diseases - Google Patents
Application of pleurotus ferulae aqueous extract in preventing and treating plant diseases Download PDFInfo
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- CN115088735A CN115088735A CN202210836181.6A CN202210836181A CN115088735A CN 115088735 A CN115088735 A CN 115088735A CN 202210836181 A CN202210836181 A CN 202210836181A CN 115088735 A CN115088735 A CN 115088735A
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- pleurotus ferulae
- biopesticide
- fire blight
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- 239000001593 sorbitan monooleate Substances 0.000 claims description 6
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- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 5
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Dentistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides an application of a pleurotus ferulae water extract in preventing and treating plant diseases, belonging to the technical field of agricultural biological prevention and treatment; the plant disease comprises a plant fungal disease and/or a plant bacterial disease. The pleurotus ferulae water extract has good inhibition effect on plant bacteria and fungal diseases, can be used for preparing biological pesticides, and is used for preventing and treating plant diseases such as pear fire blight, apple fire blight, hawthorn fire blight, tomato gray mold, grape gray mold, potato stem rot, cotton rhizoctonia solani, peanut rhizoctonia solani, rape rhizoctonia solani and the like. The pleurotus ferulae water extract is used for preventing and treating plant diseases, has small environmental harm, is not easy to generate drug resistance, and is friendly to higher animals.
Description
Technical Field
The invention belongs to the technical field of agricultural biological control, and particularly relates to an application of a pleurotus ferulae aqueous extract in plant disease control.
Background
Biopesticides are essentially living organisms themselves or biologically active substances derived from organisms. The key point for distinguishing biological pesticides from traditional chemical pesticides is whether chemical structures exist or not and whether the chemical structures are derived from organisms or not. In recent decades, the development of biological pesticides has been very rapid, and the research on biological pesticides for controlling plant diseases, insects and weeds by using biological pesticides has been successful in biological and chemical industries.
Pleurotus ferulae is also called Pleurotus ferulae and Pleurotus ferulae, belongs to Basidiomycotina, Hymenomycetes, Agaricales, Pleurotaceae and Pleurotus in taxonomy, has the mature entity of snow white, is called Pleurotus ferulae locally, belongs to Pleurotus ferulae of Basidiomycotina in Pleurotus ferulae in biophysis, is one or more extremely representative mushrooms living in grasslands under arid climate conditions, has pure white and large fruit bodies, has special fragrance and delicate and delicious taste, and is considered to be capable of resolving food stagnation and killing parasites in past generation and folk prescription and biography. Also has certain drug effect on lump in the abdomen, chronic malaria and malnutritional fatigue.
However, no report of the application of the pleurotus ferulae extract in the technical field of agricultural biological control exists at present.
Disclosure of Invention
In view of the above, the invention aims to provide the application of the water extract of the pleurotus ferulae in preventing and treating plant diseases, and the water extract of the pleurotus ferulae is developed and applied to the technical field of agricultural biological prevention and treatment.
The invention provides an application of a pleurotus ferulae water extract in preventing and treating plant diseases; the plant diseases comprise plant fungal diseases and/or plant bacterial diseases.
Preferably, the plant diseases comprise one or more of pear fire blight, apple fire blight, hawthorn fire blight, tomato gray mold, grape gray mold, potato stem rot, cotton rhizoctonia solani, peanut rhizoctonia solani and rape rhizoctonia solani.
The invention also provides a biological pesticide which comprises the pleurotus ferulae water extract and auxiliary materials.
Preferably, the mass percentage content of the pleurotus ferulae water extract in the biological pesticide is 5-30%.
Preferably, the dosage form of the biopesticide comprises an aqueous agent; the biopesticide preferably comprises the following components in percentage by weight: 5 to 30 percent of pleurotus ferulae water extract, 3 to 13 percent of surfactant, 0.1 to 0.4 percent of preservative, 0.1 to 3 percent of defoamer, 4 to 10 percent of antifreeze and the balance of water.
Preferably, the surfactant comprises one or more of benzyl dimethyl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether and sorbitan monooleate polyoxyethylene ether.
Preferably, the preservative comprises methylparaben.
Preferably, the antifreeze agent comprises glycerol and/or propylene glycol.
Preferably, the defoamer comprises a polyether defoamer.
The invention also provides the application of the biopesticide in the scheme in preventing and treating plant diseases; the plant diseases comprise plant fungal diseases and/or plant bacterial diseases; preferably, the plant diseases comprise one or more of pear fire blight, apple fire blight, hawthorn fire blight, tomato gray mold, grape gray mold, potato stem rot, cotton rhizoctonia solani, peanut rhizoctonia solani and rape rhizoctonia solani.
The invention provides an application of a pleurotus ferulae water extract in preventing and treating plant diseases; the plant disease comprises a plant fungal disease and/or a plant bacterial disease. The research of the inventor finds that the pleurotus ferulae water extract has good inhibition effect on plant bacteria and fungal diseases, can be used for preparing biological pesticide, and is used for preventing and treating plant diseases such as pear fire blight, apple fire blight, hawthorn fire blight, tomato gray mold, grape gray mold, potato stem base rot, cotton rhizoctonia solani, peanut rhizoctonia solani, rape rhizoctonia solani and the like. The pleurotus ferulae water extract is used for preventing and treating plant diseases, has small environmental harm, is not easy to generate drug resistance, and is friendly to higher animals.
Detailed Description
The invention provides an application of a pleurotus ferulae water extract in preventing and treating plant diseases; the plant diseases comprise plant fungal diseases and/or plant bacterial diseases.
In the invention, the plant diseases comprise one or more of pear fire blight, apple fire blight, hawthorn fire blight, tomato gray mold, grape gray mold, potato stem rot, cotton rhizoctonia solani, peanut rhizoctonia solani and rape rhizoctonia solani.
In the invention, the pleurotus ferulae water extract is preferably prepared by adopting the following method:
mixing the pleurotus ferulae powder and water, and performing ultrasonic oscillation extraction to obtain the pleurotus ferulae water extract.
In the invention, the ultrasonic oscillation extraction time is preferably 20-40 min, and more preferably 30 min; the ultrasonic oscillation extraction temperature is preferably 50-70 ℃, and more preferably 60 ℃; the frequency of ultrasonic oscillation extraction is preferably 25-28 khz. In the invention, the mass ratio of the pleurotus ferulae powder to the water is preferably (0.1-0.3): 50, more preferably 0.2: 50. In the present invention, the pleurotus ferulae powder is preferably obtained by air-drying and pulverizing pleurotus ferulae. After the ultrasonic oscillation extraction, preferably still standing and filtering the product of the ultrasonic oscillation extraction to obtain a pleurotus ferulae crude extract; the standing time is preferably 20-40 min, and more preferably 30 min; after the pleurotus ferulae crude extract is obtained, the invention preferably further comprises the step of concentrating the pleurotus ferulae crude extract under reduced pressure to form an extract so as to obtain a pleurotus ferulae water extract.
The invention also provides a biological pesticide which comprises the pleurotus ferulae water extract and auxiliary materials.
In the invention, the mass percentage content of the pleurotus ferulae water extract in the biological pesticide is preferably 5-30%, more preferably 10-25%, and most preferably 15-20%.
In the present invention, the formulation of the biopesticide preferably includes an aqueous agent. When the dosage form of the biological pesticide is a water aqua, the auxiliary materials preferably comprise a surfactant, a preservative, a defoaming agent, an antifreezing agent and water.
In the invention, the biopesticide preferably comprises the following components in percentage by weight: 5 to 30 percent of pleurotus ferulae water extract, 3 to 13 percent of surfactant, 0.1 to 0.4 percent of preservative, 0.1 to 3 percent of defoamer, 4 to 10 percent of antifreeze and the balance of water.
In the present invention, the biopesticide more preferably comprises the following components in percentage by weight: 15-20% of pleurotus ferulae water extract, 5-10% of surfactant, 0.2-0.3% of preservative, 0.5-1% of defoaming agent, 6-8% of antifreezing agent and the balance of water.
In the invention, the surfactant preferably comprises one or more of benzyl dimethyl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether and sorbitan monooleate polyoxyethylene ether, and more preferably sorbitan monooleate polyoxyethylene ether.
In the present invention, the preservative preferably comprises methylparaben.
In the present invention, the antifreeze agent preferably includes glycerin and/or propylene glycol.
In the present invention, the defoaming agent preferably includes polyether-based defoaming agents; the polyether defoamer is preferably a copolymer of ethylene oxide and propylene oxide available from Defeng defoamer, Inc. of Dongguan.
In the present invention, the biopesticide is preferably prepared by the following method: the preparation method comprises the following steps: mixing the water extract of the asafetida mushroom with auxiliary materials to obtain the biological pesticide. The mixing method is not particularly limited, and the uniform mixing is taken as the standard.
In the invention, the biological pesticide has the advantages of quick response, long lasting period, wide bactericidal spectrum, no toxicity, no residue and the like, and the preparation method is simple and is suitable for the requirement of large-scale industrial production.
The invention also provides the application of the biopesticide in the scheme in preventing and treating plant diseases; the plant diseases comprise plant fungal diseases and/or plant bacterial diseases; preferably, the plant diseases comprise one or more of pear fire blight, apple fire blight, hawthorn fire blight, tomato gray mold, grape gray mold, cotton rhizoctonia solani peanut rhizoctonia rot and rape rhizoctonia solani.
In the present invention, the water is preferably ultrapure water.
In the present invention, the biopesticide is preferably applied by spraying; the application frequency of the biological pesticide is preferably 2-3 times, and the interval time between two adjacent 2 times of application is preferably 7 d.
When the biological pesticide is used for preventing and controlling the pear fire blight, the biological pesticide is applied after being diluted; the dilution multiple of the biological pesticide is preferably 500-1500 times, and more preferably 1000 times; the single application dose of the diluted solution of the biological pesticide is preferably 1.5L per pear tree; the application frequency of the biological pesticide is preferably 3 times, and the application time of the biological pesticide for the first time is preferably the initial flowering period of the pear trees.
When the biological pesticide is used for preventing and treating cotton rhizoctonia rot, the dilution multiple of the biological pesticide is preferably 500-1500 times, and more preferably 1000 times; the number of application of the biopesticide is preferably 2, and the time for the first application of the biopesticide is preferably 80% after the cotton emerges.
When the biological pesticide is used for preventing and treating tomato gray mold, the dilution multiple of the biological pesticide is preferably 500-1500 times, and more preferably 1000 times; the application frequency of the biological pesticide is preferably 2 times, and the first application time of the biological pesticide is preferably the initial stage of the occurrence of the tomato gray mold.
Unless otherwise specified, the present invention does not require any particular source of the raw materials used, and commercially available products known to those skilled in the art may be used.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention.
The polyether defoamer used in the examples of the present invention was a copolymer of ethylene oxide and propylene oxide, and was obtained from delong defoaming co.
Example 1
Firstly, the pleurotus ferulae is dried and crushed to obtain the pleurotus ferulae powder. 0.2 part of the pleurotus ferulae powder is added into 50 parts of water according to the parts by mass, and the mixture is subjected to ultrasonic oscillation (60 ℃) for 30 min. Standing the obtained mixed liquid for 30min, performing suction filtration to obtain a pleurotus ferulae crude extract, and concentrating the crude extract under reduced pressure to form an extract to obtain a pleurotus ferulae water extract.
The pleurotus ferulae aqua consists of the following raw materials:
the method comprises the following specific steps: according to the formula requirements, the pleurotus ferulae water extract, sorbitan monooleate polyoxyethylene ether, methyl p-hydroxybenzoate, polyether defoamer, glycerol and water are uniformly mixed until the mixture is brown and transparent.
Example 2
The pleurotus ferulae aqua consists of the following raw materials:
the method comprises the following specific steps: according to the formula requirements, the pleurotus ferulae water extract, sorbitan monooleate polyoxyethylene ether, methyl p-hydroxybenzoate, polyether defoamer, glycerol and water are uniformly mixed until the mixture is brown and transparent.
Example 3
See example 1 for specific steps.
Example 4:
see example 1 for specific procedures.
Example 5:
see example 1 for specific procedures.
Example 6:
see example 1 for specific procedures.
Example 7:
see example 1 for specific procedures.
Example 8:
see example 1 for specific procedures.
Test example 1: indoor biological assay for pleurotus ferulae water extract
The pleurotus ferulae aqueous extract prepared in the embodiment 1 has better inhibition effect on botrytis cinerea, rhizoctonia solani and erwinia amylovora, wherein the virus power of the pleurotus ferulae aqueous extract is strongest, and EC is higher than that of the pleurotus ferulae aqueous extract 50 Reaches 82.36mg/L, has lower toxicity to botrytis cinerea, but EC 50 The value can still reach 118.81 mg/L.
The experimental process is as follows, the botrytis cinerea and rhizoctonia solani strains are inoculated into a PDA culture medium and a PDA culture medium with drugs of different concentrations, the diameter of a colony is measured by a cross method after 3d of culture, the operation is repeated for three times, the inhibition activity of the pleurotus ferulae water extract on the botrytis cinerea and the rhizoctonia solani under different concentration gradients is measured by the diameter of the colony, and a virulence curve is obtained. Inoculating pear fire blight bacterium strains into KB culture medium and KB culture medium with drugs of different concentrations, shaking at 160r/min for 24h, measuring bacterium liquid turbidity, repeating for three times, measuring the inhibition activity of the pleurotus ferulae water extract on the fire blight under different concentration gradients through the bacterium liquid turbidity, and obtaining a toxicity curve.
The results of the indoor activity determination of the pleurotus ferulae water extract on tomato gray mold, rhizoctonia solani and erwinia amylovora are shown in the table:
TABLE 1 inhibition of pathogenic bacteria by aqueous extracts of Pleurotus ferulae Lanzi
Test example 2: 500-time, 1000-time and 1500-time 5% asafetida mushroom aqua field drug effect test for preventing and treating pear fire blight
In 2021, the control effect of the 5% asafetida mushroom aqua on the pear fire blight was measured in the bergamot pear garden in Kuerle region of Xinjiang. In the area where the fire blight of the Korla pears occurs, a pear garden with uniform growth vigor and uniform disease occurrence is selected, the tree age is 25-35 years, every two trees are repeated, the tree age is four times, and 1.5L of liquid medicine is sprayed on each tree. The application is started at the initial flowering phase of the pear trees, and the application is continuously carried out for 3 times and 7 days per time. The test results are investigated 7-14 days after the last application.
Each tree is subjected to fixed-point survey at 5 parts of east, west, south, north and middle parts, the proportion of the disease area of 10 current-year branches in each point to the whole branch is surveyed, and the number of the treated branches and the corresponding disease grade are recorded.
The diseased branch grading criteria were as follows:
grade 0, no occurrence, no disease spots on the branches and the trunks;
grade 1, the disease area of the branches accounts for less than 5% of the whole branches;
grade 3, the disease area of the branches accounts for 6 to 15 percent of the whole branch;
grade 5, the disease area of the branches accounts for 16-30% of the whole branch;
7, the disease area of the branches accounts for 31 to 50 percent of the whole branch;
grade 9, the disease area of the branch accounts for more than 50% of the whole branch.
The control effect calculation method comprises the following steps:
the field test result shows that: the 5 percent asafetida mushroom aqua has better control effect on the pear fire blight within the test concentration range. 7 days after the last application, the control effects of the 500-time and 1000-time liquid 5% pleurotus ferulae water aqua treatment groups are 74.00% and 68.83% respectively, and are both obviously higher than the control group 1500-time liquid 2% kasugamycin water aqua. 14 days after the last medicine, the 5 percent of asafetida mushroom aqua still has better control effect on the pear fire blight, and the control effect of the 5 percent of asafetida mushroom aqua on the pear fire blight is 63.58 percent when the dosage of the preparation is 500 times of the liquid, which is obviously higher than that of a control group. The 5% Pleurotus ferulae water aqua treated group with preparation dosage of 1000 and 1500 times of solution has equivalent effect to the control group.
TABLE 25 field drug effect of asafetida mushroom aqua on pear fire blight
Test example 3: 500-time, 1000-time and 1500-time 5% asafetida mushroom aqua field drug effect test for preventing and treating cotton rhizoctonia rot
The eighth 143 of the military consortium in Xinjiang province selected cotton fields with consistent soil shape, uniform fertility and consistent onset of cotton blight all the year round for test.
The application is started 80% after cotton emergence, and the application is continuously carried out for 2 times at intervals of 7 days. And (5) investigating results 7 days after the last pesticide application, sampling five points in each cell, fixing 20 cotton seedlings in each point, and investigating the occurrence condition of damping off.
The cotton rhizoctonia solani grading method comprises the following steps:
level 0: the base of the stem has no disease spots;
level 1: the lesion at the base of the stem accounts for less than 1/3 of the whole circumference of the stem;
and 3, level: the lesion spots at the base of the stem account for 1/3-1/2 of the whole circumference of the stem;
stage 5: the lesion spots at the base of the stem account for 1/2-3/4 of the whole circumference of the stem;
and 7, stage: the lesion at the base of the stem is more than 3/4 of the whole circumference of the stem.
In the formula:
CK 0 - -index of pre-application disease in placebo zone
CK 1 - -disease index after drug administration in placebo zone
PT 0 - -index of pre-application disease in the agent treatment area
PT 1 - -disease index after administration to the agent treatment area
The field test result shows that: the 5% asafetida mushroom aqua has high effect of preventing and treating cotton rhizoctonia rot. Wherein the control effect of the 500 times liquid treatment group of the 5 percent pleurotus ferulae aqua is 78.53 percent, which is obviously superior to 3 hundred million CFU/gram trichoderma harzianum wettable powder of 300 times liquid of a contrast medicament; the control effect of the 1000-time liquid and 1500-time liquid of 5% asafetida mushroom aqueous solution treatment group on cotton rhizoctonia solani is 70.01% and 63.13% respectively, and the control effect is equivalent to that of a control medicament.
TABLE 3 field effect of asafetida mushroom aqua on cotton rhizoctonia rot
Test example 4: 500-time, 1000-time and 1500-time 5% asafetida mushroom aqua pesticide effect test in field for preventing and treating tomato gray mold
In 2021, a field pesticide effect test of 5% of asafetida mushroom aqua for preventing and treating tomato gray mold is carried out in a greenhouse of Xinjiang river city. The application is started at the early stage of the occurrence of the tomato gray mold, and the application is continuously carried out for 2 times at intervals of 7 days. The disease condition was investigated before the first dose and 7 days after each dose.
Sampling is carried out at five points in each cell, 2-3 plants are investigated at each point, all leaves and fruits of each plant are investigated, the disease and fruit rate is calculated, and the disease and fruit rate is recorded according to the following classification method.
Leaf damage grading method (taking leaves as a unit):
level 0: no lesion spots;
level 1: 3 scabs exist on a single leaf;
and 3, level: 4-6 scabs exist on a single leaf;
and 5, stage: 7-10 scabs exist on a single leaf;
and 7, stage: 11-20 scabs exist in a single leaf, and the parts are densely divided into pieces;
and 9, stage: the single leaf has dense lesion spots occupying more than one fourth of the leaf area.
Tomato fruit damage grading method (taking fruit as unit):
level 0: no disease spots;
level 1: residual petal morbidity or stigma morbidity;
and 3, stage: the sepal decay or the stigma attack spreads to the hilum of the fruit;
and 5, stage: the fruit umbilicus part has an infiltration spot and mildew-free layer;
and 7, stage: the fruit umbilicus part has a mildew layer but does not extend to other parts;
and 9, stage: the mould layer extends to other parts of the fruit.
In the formula:
CK 0 - -Pre-drug index of blank control area
CK 1 - -disease index after drug administration in placebo zone
PT 0 - -pre-application disease index of the agent treatment area
PT 1 - -disease index after administration to the agent treatment area
The field test result shows that: the 5% asafetida mushroom aqua has high tomato gray mold preventing and treating effect. 7 days after the first pesticide application, the leaf control effect and the fruit control effect of the 5 percent pleurotus ferulae aqua with 500 times of liquid on the tomato gray mold are respectively 75.12 percent and 76.77 percent, which are both obviously higher than 3 hundred million CFU/gram trichoderma harzianum wettable powder of 300 times of liquid in a control group; 7 days after the second application, the leaf control effect of 5% of the asafetida mushroom aqua with 500 times of liquid on the tomato gray mold is 86.13%, which is obviously higher than that of the control group; the control effect of the 500-time liquid and the 1000-time liquid of 5% pleurotus ferulae water aqua on the tomato gray mold is 83.52% and 73.79% respectively, which are both equivalent to the effect of a control medicament.
TABLE 4 field control of aqueous Pleurotus ferulae solution for tomato Gray mold
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (10)
1. The application of the pleurotus ferulae water extract in preventing and treating plant diseases, wherein the plant diseases comprise plant fungal diseases and/or plant bacterial diseases.
2. The use of claim 1, wherein the plant disease comprises one or more of a pear fire blight, an apple fire blight, a hawthorn fire blight, a tomato gray mold, a grape gray mold, a potato stem rot, a cotton rhizoctonia rot, a peanut rhizoctonia rot and a rape rhizoctonia rot.
3. A biological pesticide comprises Pleurotus Ferulae water extract and adjuvants.
4. The biological pesticide as claimed in claim 3, wherein the mass percentage content of the Pleurotus ferulae water extract in the biological pesticide is 5-30%.
5. The biopesticide of claim 4, wherein the formulation of the biopesticide comprises an aqueous agent; the biopesticide preferably comprises the following components in percentage by weight: 5 to 30 percent of pleurotus ferulae water extract, 3 to 13 percent of surfactant, 0.1 to 0.4 percent of preservative, 0.1 to 3 percent of defoaming agent, 4 to 10 percent of antifreezing agent and the balance of water.
6. The biopesticide of claim 5, wherein the surfactant comprises one or more of benzyl dimethyl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether and sorbitan monooleate polyoxyethylene ether.
7. Biopesticide according to claim 5, characterized in that the preservative comprises methylparaben.
8. Biopesticide according to claim 5, characterized in that the antifreeze agent comprises glycerol and/or propylene glycol.
9. The biopesticide of claim 5, wherein the antifoaming agent comprises a polyether-based antifoaming agent.
10. Use of the biopesticide of any one of claims 3 to 9 for controlling plant diseases; the plant diseases comprise plant fungal diseases and/or plant bacterial diseases; preferably, the plant diseases comprise one or more of pear fire blight, apple fire blight, hawthorn fire blight, tomato gray mold, grape gray mold, potato stem rot, cotton damping-off, peanut damping-off and rape damping-off.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800345A (en) * | 2005-12-15 | 2006-07-12 | 孔岩 | Culture medium for pleurotus ferulae invigoration |
| CN101913930A (en) * | 2010-08-05 | 2010-12-15 | 孔岩 | Method for producing biological organic fertilizer from pleurotus ferulae waste auriculariabran |
| WO2013089429A1 (en) * | 2011-12-12 | 2013-06-20 | 경상북도 | Composition for preventing or treating hyperlipidemia containing water extract of pleurotus eryngii var. ferulae as active ingredient |
| KR101458496B1 (en) * | 2014-03-28 | 2014-11-07 | 주식회사 아미코스메틱 | A skin-care agent for anti-inflammatory containig Pleurotus ferulae fruit body extract or Pleurotus ferulae mycelium extract or Pleurotus ferulae mycelium culture fluid |
| CN105831175A (en) * | 2016-04-29 | 2016-08-10 | 鞍山禾瑞生物科技有限公司 | Liquid compound biological multi-control fungicide and preparation method and application thereof |
| CN105917958A (en) * | 2016-04-15 | 2016-09-07 | 新疆农业科学院微生物应用研究所 | Method for researching interaction between Pleurotus ferulae fungi and eremophyte ferula asafoetida seed germination |
| CN105993587A (en) * | 2016-05-13 | 2016-10-12 | 新疆农业科学院微生物应用研究所 | Method for studying influence of pleurotus ferulae mushroom fungus liquid on desert plant ferula seedling growth |
| CN108558499A (en) * | 2018-04-09 | 2018-09-21 | 山东省农业科学院农业资源与环境研究所 | A kind of compound formulation for melon crop of the extract containing edible fungi residue |
-
2022
- 2022-07-15 CN CN202210836181.6A patent/CN115088735A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800345A (en) * | 2005-12-15 | 2006-07-12 | 孔岩 | Culture medium for pleurotus ferulae invigoration |
| CN101913930A (en) * | 2010-08-05 | 2010-12-15 | 孔岩 | Method for producing biological organic fertilizer from pleurotus ferulae waste auriculariabran |
| WO2013089429A1 (en) * | 2011-12-12 | 2013-06-20 | 경상북도 | Composition for preventing or treating hyperlipidemia containing water extract of pleurotus eryngii var. ferulae as active ingredient |
| KR101458496B1 (en) * | 2014-03-28 | 2014-11-07 | 주식회사 아미코스메틱 | A skin-care agent for anti-inflammatory containig Pleurotus ferulae fruit body extract or Pleurotus ferulae mycelium extract or Pleurotus ferulae mycelium culture fluid |
| CN105917958A (en) * | 2016-04-15 | 2016-09-07 | 新疆农业科学院微生物应用研究所 | Method for researching interaction between Pleurotus ferulae fungi and eremophyte ferula asafoetida seed germination |
| CN105831175A (en) * | 2016-04-29 | 2016-08-10 | 鞍山禾瑞生物科技有限公司 | Liquid compound biological multi-control fungicide and preparation method and application thereof |
| CN105993587A (en) * | 2016-05-13 | 2016-10-12 | 新疆农业科学院微生物应用研究所 | Method for studying influence of pleurotus ferulae mushroom fungus liquid on desert plant ferula seedling growth |
| CN108558499A (en) * | 2018-04-09 | 2018-09-21 | 山东省农业科学院农业资源与环境研究所 | A kind of compound formulation for melon crop of the extract containing edible fungi residue |
Non-Patent Citations (3)
| Title |
|---|
| 宋旭红等: ""新疆阿魏蘑菇提取物体外抗肿瘤实验研究"", 《癌变•畸变•突变》, vol. 14, no. 2 * |
| 宋旭红等: ""新疆阿魏蘑菇提取物体外抗肿瘤实验研究"，宋旭红等，《癌变•畸变•突变》", 《癌变•畸变•突变》, vol. 14, no. 2, pages 107 - 110 * |
| 张伟丰等: ""阿魏菇多糖聚酰胺层析脱色工艺及其抑菌活性"", 《安徽农业科学》, vol. 43, no. 14, pages 33 - 35 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116491513A (en) * | 2023-05-06 | 2023-07-28 | 江西省农业科学院植物保护研究所 | Pharmaceutical composition and application thereof in prevention and treatment of asparagus stem blight |
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