CN107746296A - A kind of preparation method of alga fertilizer - Google Patents
A kind of preparation method of alga fertilizer Download PDFInfo
- Publication number
- CN107746296A CN107746296A CN201710706522.7A CN201710706522A CN107746296A CN 107746296 A CN107746296 A CN 107746296A CN 201710706522 A CN201710706522 A CN 201710706522A CN 107746296 A CN107746296 A CN 107746296A
- Authority
- CN
- China
- Prior art keywords
- sea
- tangle
- residue
- revs
- pueraria root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
Abstract
A kind of preparation method of alga fertilizer, using sea-tangle leftover pieces as raw material, the pueraria root residue process for fixation of preprocess method and enzyme is combined by colloid mill and high pressure homogenizer, obtained marine alga is homogenized through immobilised enzymes two step method enzymolysis process Biocatalytic Conversion, realize that algal polysaccharides and protein are degraded into the target of alga oligosaccharides and polypeptide respectively, the final continuous production processes established the sea-tangle enzymolysis based on continuous flowing stirred tank and prepare alga fertilizer, the centrifuged supernatant of enzymolysis liquid and sea-tangle residue, supernatant is through falling film evaporation into concentrate, ultrafiltrate is liquid seaweed extract；Sea-tangle digests the common fermentation such as residue and cake of camellia oleifera seeds into solid organic fertilizer, reaches the purpose that the full cell of sea-tangle utilizes.The present invention improves the sea-tangle level of resources utilization, improves kelp processing technology and the alga fertilizer level of production, realizes that enzyme is repeated and is used continuously, reduces the consumption of enzyme, reduce alga fertilizer production cost, product quality is good, pure natural, pollution-free degradable.
Description
Technical field
The invention belongs to chemical technology field, it is related to the preparation method of marine alga fertilizer.
Background technology
Marine alga fertilizer is the bio-feritlizer be processed into through science using sea-plant marine alga as primary raw material, mainly into
Divide is that the natural bioactivity substance for being advantageous to growth and development of plants and marine alga extracted from marine alga absorbs simultaneously richness from ocean
The mineral nutrient element of collection in vivo, including algal polysaccharides, phenols poly-compounds, mannitol, glycine betaine, plant growth are adjusted
Save material (basic element of cell division, gibberellin, auxin and abscisic acid etc.) and the micro member such as nitrogen, phosphorus, potassium and iron, boron, molybdenum, iodine
Element.In addition, being increase fertilizer efficiency and the chelation of fertilizer, appropriate humic acid and appropriate trace element have also been dissolved in.
Compared with conventional fertilizers, alga fertilizer has following distinguishing feature：
First, alga fertilizer is more comprehensive than conventional fertilizers nutrition, balanced, obvious effect of increasing production is grown after crop applying, and seldom lack
Plain disease.Alga fertilizer contains the day for being advantageous to growth and development of plants largely extracted from marine alga using natural seaweed as primary raw material
Right bioactive substance and marine alga absorb from ocean and are enriched with mineral nutrient element in vivo, including algal polysaccharides, phenol
Class poly-compounds, mannitol, glycine betaine, plant growth regulating substance（The basic element of cell division, gibberellin, auxin and abscisic acid
Deng）With the trace element such as nitrogen, phosphorus, potassium and iron, boron, molybdenum, iodine.In addition, be increase fertilizer efficiency and fertilizer chelation, portioned product
Appropriate humic acid and appropriate trace element have also been dissolved in, can meet the needs of plant growth each stage is to nutrition.2nd, alga fertilizer
In contain a large amount of disease-resistant factors and special composition, fertile medicine economic benefits and social benefits.Degeneration-resistant disease resistance significantly increases after crop applying, and foliage-spray can
Improve pesticidal effects.The algal polysaccharides and oligosaccharide that contain in alga fertilizer, mannitol, phenols poly-compounds, glycine betaine, marine alga
The material such as acid and natural antibiotics, has significant inhibitory anti-virus, anthelminthic effect, really plays fertile medicine effect, significantly strengthens
The cold-resistant of crop, drought resisting, disease-resistant, resistant to lodging, salinity and alkalinity resistance, to epidemic disease, virosis, anthrax, downy mildew, grey mold, powdery mildew, withered
The stronger resistance of the generations such as disease of withering.In addition, the alginic acid in alga fertilizer can reduce the surface tension of water, formed in plant surface
Thin film, increase contact area, make water-soluble substances be easier to enter plant cell through cauline leaf superficial cell film, make plant
Thing most effectively absorbs the nutritional ingredient in seaweed extracted liquor.If alga fertilizer and insecticide, bactericide are used in mixed way, have
Synergistic effect, sprinkling expense can be reduced.3rd, substantial amounts of high active ingredient is contained in alga fertilizer, plant easily absorbs.After crop applying
Growing way is vigorous, can significantly improve yield and the quality of crop.Specific algal polysaccharides, alginic acid, height are unsaturated in marine alga
The materials such as aliphatic acid, there is very high bioactivity, the generation of the nonspecific activity factor in plant can be stimulated.Meanwhile sea
Also contain natural plants growth regulator in algae fertilizer, such as：Auxin, cytokinin-like substance and gibberellin etc., its ratio with
Each hormone ratio is close in terrestrial plant, has the function that to adjust Endogenous hormone balance very well.After crop applying, yield and quality
Significantly improve, the industrial crops quality such as tobacco, cotton, flowers can be significantly improved, especially to booth vegetable crop, increased both production and value
Effect is very notable.4th, alga fertilizer contains abundant organic matter and the sustained release factor, fertilizer efficiency length, can improve soil micro-ecosystem, soil living
Promoting root growth and anti-continuous cropping.Alga fertilizer can directly make soil or soil is increased organic matter by plant, activate various having in soil
Beneficial microorganism.These microorganisms can play a part of catalyst in plant, microbial metabolism circulation, make the biopotency of soil
Increase.The metabolin of plant and edaphon can provide more nutrients for plant.Meanwhile the chelating system that algal polysaccharides are formed
System can be such that nutrition slowly discharges, and extend fertilizer efficiency.In addition, the native compound such as sodium alginate that it contains is natural soil conditioning
Agent, the formation of soil granular structure can be promoted, improve soil internal void space, coordinate solid, liquid, gas proportions in soil,
Recover due to soil over-burden and chemical contamination and lose lac balance, increase geobiont vigor, promote it is quick-acting
The release of nutrient, is advantageous to root growth, improves the resistance of crop and anti-continuous cropping ability.5th, the natural safety of alga fertilizer is without public affairs
Evil.Traditional chemical fertilizer fertilizer efficiency is single, seriously polluted, and long-term use can cause soil texture to be destroyed.Alga fertilizer category is natural
Marine algae extract, there is good compatibility with terrestrial plant, it is nontoxic to people, animal, it is environmentally safe, have other any
The advantages of chemical fertilizer is all incomparable, it is put into organic food special fertilizer material abroad.Alga fertilizer has polymerize a variety of in ocean
Active material, possess comprehensive nutrition, disease-resistant yield-increasing, live many advantages, such as native, nuisanceless, be really to meet modern agricultural development side
To good fertilizer.
China's alga fertilizer industry is started late, and phase late 1990s begins one's study, into 21 century after obtained it is fast
Speed development.In terms of the mode of production, mainly there are chemical method, physical method, 3 kinds of biological method.Currently, domestic most manufacturing enterprises
Main chemical leaching test production alga fertilizer simple using cheap, is that highly basic high temperature can destroy using the maximum inferior position of chemical method
The activity of marine alga endogenous substance；Physics extraction method is better than chemical leaching test, and it is thin that the technique cooled down using high pressure low temperature reaches marine alga
The broken purpose of cell wall, obtained macromolecular substances are difficult to directly be absorbed by crop, and fertilizer efficiency is not high；Most preferably biological method,
Extracted using enzyme hydrolysis, the activity of endogenous substance can be made to preserve to greatest extent.Due to the costliness of biology enzyme price
And the complexity of enzymolysis process, the country only have several families to take bioanalysis i.e. enzyme extraction.
Develop alga fertilizer, China has raw material advantage, and about 1,500,000 tons of the annual marine alga total output in China, 80% above is artificial
Cultivation, the marine alga quantity currently used for system fertilizer is seldom, no more than the 10% of marine alga total amount；And China is large agricultural country, fertilizer demand
Amount is very huge, therefore the development space of alga fertilizer industry is also very big.Sea-tangle leftover pieces are the processes such as laminaria production processed food
In residue is useless, particle, currently without being fully used, mainly as offal treatment.This had both caused environmental pollution and asked
Topic, result in the serious waste of sea-tangle resource again.And sea-tangle is the quality raw materials for producing alga fertilizer.On the one hand it is environmental protection,
Solves problem of environmental pollution caused by the abandonment of sea-tangle leftover pieces；On the other hand alga fertilizer production cost is reduced；Develop sea-tangle corner
Expect that Biocatalytic Conversion alga fertilizer technical meaning is great.
The content of the invention
Environmental pollution and the wasting of resources, marine alga are caused for sea-tangle leftover pieces are discarded during laminaria production processed food etc.
Fertile raw materials for production cost is high, the problems such as full cell utilizes is not implemented in marine alga in alga fertilizer production process.The mesh of the present invention
Be a kind of preparation method of alga fertilizer is provided, using sea-tangle leftover pieces as raw material, combined through colloid mill and high pressure homogenizer pre-
After processing, by establishing immobilised enzymes two step method enzymolysis process, realize that alga fertilizer connects in continuously flowing stirred tank
Continuous production and enzyme recycling, improve production efficiency, reduce the cost of enzyme；Caused sea-tangle residue and Oil-tea-cake in enzymolysis process
The fermenting and producing solid-state seaweed organic fertilizer such as dregs of rice, this method realize that the full cell of sea-tangle leftover pieces utilizes, greatly reduces consuming, greatly for enzyme
Width reduce alga fertilizer production cost, produce alga fertilizer is pure natural, degradable, quality is good, environmental protection, the reduction wasting of resources.
The purpose of the present invention is achieved through the following technical solutions.
A kind of preparation method of alga fertilizer of the present invention, according to the following steps.
(1) sea-tangle waste pre-processes：Sea-tangle leftover pieces are cleaned with running water；Soaked 24 hours with running water again,
Every 3 hours of period changed water 1 time；Leftover pieces after immersion are shredded with knife, and broken sea-tangle crushes through colloid mill.Kelp paste is with steaming after crushing
Distilled water is with volume ratio 1:5 ~ 20 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：40 ~ 60 MPas of pressure, is followed
Ring number 3 ~ 5 times, 1000 ls/h of flow, room temperature condition.
(2) first step enzyme digestion reaction：Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:5 ~ 20 mix, the company of being placed in
Afterflow is moved in stirred tank, and cellulase is placed in together with agitating shaft through pueraria root residue immobilization, immobilized cellulase
The wire netting inframe of rotation, according to kelp residue and cellulase mass ratio 10 ~ 100:1 addition immobilized cellulase, adjusts pH
To 5.5 ~ 6.5,100 ~ 200 revs/min of speed of agitator, after stirring 10 ~ 30 minutes, speed of agitator is adjusted to 50 ~ 150 revs/min,
Temperature control is at 45 ~ 65 DEG C；After immobilized cellulase Biocatalytic Conversion 2 ~ 3 hours, start continuous feed and discharging, reaction
Stop charging and discharging within 1 ~ 3 hour before end.
(3) second step enzyme digestion reaction：Discharging enters in another continuous flowing stirred tank in step (2), alkalescence
Protease is through pueraria root residue immobilization, and immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by alkalescence
The mg/litre of protease concentration 300 ~ 120000 add immobilization alkali protease, adjust pH to 5.0 ~ 7.0, by 100 ~ 200 turns/
After minute stirring 8 ~ 24 minutes, speed of agitator is adjusted to 50 ~ 150 revs/min, and temperature control is at 50 ~ 70 DEG C；Reaction starting 2 ~ 3
After hour, start continuous feed and discharging, reaction terminates to stop charging and discharging in first 1 ~ 3 hour.
(4) by the enzymolysis liquid that step (3) obtains through centrifugation（5000 ~ 12000 revs/min, 5 ~ 15 minutes, room temperature condition）
Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, 20 ~ 100 ls/h of evaporation capacity, temperature 50 ~
70 DEG C, 0.08 ~ 0.30 MPa of pressure, cocnentration factor 1.3 ~ 1.8, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, pressure
0.4 MPa of power, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.
(5) by the sea-tangle residue in step (4) and cake of camellia oleifera seeds, pericarp, waste dish, brewage waste residue, chicken manure with mass ratio 1:
1~3:3~5:3~5:1~2:1 ~ 4 heap fermentation, 4 ~ 10 cubic metres of volume, 50 ~ 90 centimetres of height, 50 ~ 70 DEG C of temperature, by translating into
Fermentation materials control temperature, fermentation time 20 ~ 40 days.Fermentation ends obtain high-quality solid organic fertilizer.
Continuous feed and discharge method are taken in step (2) of the present invention, speed is consistent, for 0.5 ~ 4 kilogram/it is small
When.
Pueraria root residue described in step (2) of the present invention and (3), preprocess method are as follows：Caused by kudzu-vine root powder production process
Pueraria root residue distilled water flushing 3 ~ 5 times, is placed in beaker, and pueraria root residue is with 10% ~ 40% Eusol with mass ratio 1:5 ~ 15 is mixed
It is even, vibrated in shaking table, after being vibrated 10 ~ 30 minutes by 100 ~ 200 revs/min, adjustment of rotational speed is 50 ~ 150 revs/min, temperature
Control is at 30 ~ 50 DEG C, duration of oscillation 24 ~ 48 hours, and vibration terminates rear pueraria root residue distilled water flushing 3 ~ 5 times.
Immobilized cellulase described in step (2) of the present invention, process for fixation are as follows：Cellulase is dissolved in 0.05
~ 0.5 mol/L phosphate buffer, pH of cushioning fluid are 5.5 ~ 7.0, and enzyme concentration is 5 ~ 50 g/l；After pretreatment pueraria root residue with
Cellulase solution is with mass ratio 1:5 ~ 20 mix, and are vibrated in shaking table, after being vibrated 10 ~ 30 minutes by 100 ~ 150 revs/min,
Adjustment of rotational speed is 50 ~ 100 revs/min, and at 25 ~ 40 DEG C, duration of oscillation 24 ~ 48 hours, vibration secures temperature control after terminating
The pueraria root residue of cellulase distilled water flushing 3 ~ 5 times.
Continuous feed and discharge method are taken in step (3) of the present invention, speed is consistent, and is 0.5 ~ 4 kg/hr.
Immobilization alkali protease described in step (3) of the present invention, enzyme immobilizatio method are as follows：Alkali protease is molten
For solution in 0.05 ~ 0.5 mol/L phosphate buffer, pH of cushioning fluid is 5.5 ~ 7.0, and enzyme concentration is 1 ~ 50 g/l；After pretreatment
Pueraria root residue and alkaline protease solution with mass ratio 1:5 ~ 20 mix, and are vibrated in shaking table, by 100 ~ 150 revs/min of vibrations
After 10 ~ 30 minutes, adjustment of rotational speed is 50 ~ 100 revs/min, and temperature control duration of oscillation 24 ~ 48 hours, vibrates at 25 ~ 50 DEG C
The pueraria root residue of alkali protease distilled water flushing 3 ~ 5 times is secured after end.
First step enzyme digestion reaction described in step (2) of the present invention, mainly by the algal polysaccharides in the structures such as sea-tangle cell membrane
The expression of the differential proteins such as crop can be induced by changing into the products such as the high oligosaccharides of the utilization ratios such as crop, alginic acid, and preventing and treating is made
The related disease such as thing.
Second step enzyme digestion reaction described in step (3) of the present invention, primarily to sea-tangle protein is catalytically converted into spy
The products such as specific polypeptide, amino acid.
Pericarp described in step (5) of the present invention is banana skin, watermelon peel or apple skin, waste dish be Chinese cabbage, romaine lettuce or
Lettuce waste dish, it is brewed spirit waste residue to brewage waste residue.
The present invention establishes a kind of preparation method of alga fertilizer, based on immobilised enzymes two step method catalyzed conversion sea-tangle corner
Material, finally establishes the continuous production processes that sea-tangle enzymolysis prepares alga fertilizer, and enzymolysis liquid kills centrifuged supernatant and sea after enzyme
Band residue, supernatant through falling film evaporation into concentrate be liquid seaweed extract；Sea-tangle digest the common fermentation such as residue and cake of camellia oleifera seeds into
Solid organic fertilizer, reach the purpose that the full cell of sea-tangle utilizes.Real kelp processing waste recycling, improve sea-tangle resource profit
With efficiency, environment is protected, realizes that enzyme is repeated and is used continuously, greatly reduces the consumption of enzyme, greatly reduce alga fertilizer production
Cost, practicality is very strong, is easy to scale, and it is good and be all-natural product to produce alga fertilizer quality, pollution-free degradable, is one
Kind meets industrialization demand, environmentally friendly new method.
Embodiment
The present invention will be described further by following examples.
Sea-tangle leftover pieces and cake of camellia oleifera seeds used in following examples are commercially available.
Pericarp and waste dish used in following examples are respectively collected from fruit wholesale market and the market of farm produce.
Chicken manure used in following examples is collected from chicken farm.
Cellulase and alkali protease used in following examples are purchased from Sigma-Aldrich companies.
Embodiment 1.
Sea-tangle leftover pieces are cleaned with running water, then soaked 24 hours with running water, change water 1 time within during which every 3 hours；Immersion
Leftover pieces afterwards are shredded (wide with knife：0.5 cm, it is long：5 cms), broken sea-tangle crushes through colloid mill.Sea-tangle after crushing
Mud is with distilled water with volume ratio 1:5 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：40 MPas of pressure, is followed
Ring number 3 times, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：With steaming
Distilled water is rinsed 3 times, is placed in beaker, and pueraria root residue is with 10% Eusol with mass ratio 1:5 mix, and vibrate, press in shaking table
After 100 revs/min vibrate 10 minutes, adjustment of rotational speed is 50 revs/min, and temperature control duration of oscillation 24 hours, vibrates at 30 DEG C
Pueraria root residue distilled water flushing 3 times after end, for immobilised enzymes.Cellulase immobilization method：Cellulase is dissolved in
0.05 mol/L phosphate buffer, pH of cushioning fluid 5.5, enzyme concentration are 5 g/l；Pueraria root residue and cellulase after pretreatment
Solution is with mass ratio 1:5 mix, and are vibrated in shaking table, and after being vibrated 10 minutes by 100 revs/min, adjustment of rotational speed is 50 revs/min
Clock, for temperature control at 25 DEG C, duration of oscillation 24 hours, vibration secures the pueraria root residue distilled water flushing of cellulase after terminating
3 times.The process for fixation of alkali protease is as follows：Alkali protease is dissolved in 0.05 mol/L phosphate buffer, pH of buffer
It is worth for 5.5, enzyme concentration is 1 g/l；Pretreated pueraria root residue is with alkaline protease solution with mass ratio 1:5 mix, in shaking table
Middle vibration, after 100 revs/min of vibrations 10 minutes, adjustment of rotational speed is 50 revs/min, and temperature control is at 25 DEG C, duration of oscillation 24
Hour, vibration secures the pueraria root residue of alkali protease distilled water flushing 3 times after terminating.
Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:5 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to kelp residue and enzyme mass ratio 10:1
Add immobilised enzymes, adjust pH to 5.5,100 revs/min of speed of agitator, stirring 10 minutes after, speed of agitator be adjusted to 50 turns/
Minute, temperature control is at 45 DEG C；After immobilised enzymes Biocatalytic Conversion 2 hours, start continuous feed and discharging, input and output material speed
It it is 0.5 kg/hr, reaction terminates to stop charging and discharging in first 1 hour.Discharging enters another 50 liters continuous flowing agitator tanks
In formula reactor, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by 300 milligrams of enzyme concentration/
Addition immobilised enzymes is risen, adjusts pH to 5.0, after being stirred 8 minutes by 100 revs/min, speed of agitator is adjusted to 50 revs/min, temperature
Degree control is at 50 DEG C；After reaction starting 2 hours, it is 0.5 kg/hr to start continuous feed and discharging, input and output material speed, instead
It should terminate to stop charging and discharging in first 1 hour.The enzymolysis liquid of discharging is through centrifugation（5000 revs/min, 5 minutes, room temperature condition）
To supernatant and sea-tangle residue, supernatant falling film evaporation technology is concentrated, 20 ls/h of evaporation capacity, temperature 50 C, pressure
0.08 MPa, cocnentration factor 1.3, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure, cross-flow rate
3.6 meter per seconds, 800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Sea-tangle residue and cake of camellia oleifera seeds, banana skin, great Bai
Dish waste dish, brewed spirit waste residue, chicken manure are with mass ratio 1:1:3:3:1:1 heap fermentation, 4 cubic metres of volume, 50 centimetres of height, temperature
50 DEG C of degree, temperature, fermentation time 20 days are controlled by translating into fermentation materials.Fermentation ends obtain high-quality solid organic fertilizer.
Embodiment 2.
Sea-tangle leftover pieces are cleaned with running water, then soaked 24 hours with running water, change water 1 time within during which every 3 hours；Immersion
Leftover pieces afterwards are shredded (wide with knife：0.5 cm, it is long：5 cms), broken sea-tangle crushes through colloid mill.Sea-tangle after crushing
Mud is with distilled water with volume ratio 1:20 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：60 MPas of pressure,
Cycle-index 5 times, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：With
Distilled water flushing 5 times, is placed in beaker, and pueraria root residue is with 40% Eusol with mass ratio 1:15 mix, and are vibrated in shaking table,
After 200 revs/min of vibrations 30 minutes, adjustment of rotational speed is 150 revs/min, and temperature control is at 50 DEG C, duration of oscillation 48 hours,
Vibration terminates rear pueraria root residue distilled water flushing 5 times, for immobilised enzymes.Cellulase immobilization method：Cellulase dissolves
In 0.5 mol/L phosphate buffer, pH of cushioning fluid 7.0, enzyme concentration is 50 g/l；Pueraria root residue and cellulose after pretreatment
Enzyme solutions are with mass ratio 1:20 mix, vibrated in shaking table, by 150 revs/min vibration 30 minutes after, adjustment of rotational speed be 100 turns/
Minute, temperature control at 40 DEG C, with distilled water rushed by duration of oscillation 48 hours, vibration secures cellulase pueraria root residue after terminating
Wash 5 times.The process for fixation of alkali protease is as follows：Alkali protease is dissolved in 0.5 mol/L phosphate buffer, buffer solution
PH value is 7.0, and enzyme concentration is 50 g/l；Pretreated pueraria root residue is with alkaline protease solution with mass ratio 1:20 mix,
Vibrated in shaking table, after being vibrated 30 minutes by 150 revs/min, adjustment of rotational speed is 100 revs/min, and temperature control is at 50 DEG C, vibration
48 hours time, vibration secure the pueraria root residue of alkali protease distilled water flushing 5 times after terminating.
Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:20 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to kelp residue and enzyme mass ratio 100:1
Add immobilised enzymes, adjust pH to 6.5,200 revs/min of speed of agitator, stirring 30 minutes after, speed of agitator be adjusted to 150 turns/
Minute, temperature control is at 65 DEG C；After immobilised enzymes Biocatalytic Conversion 3 hours, start continuous feed and discharging, input and output material speed
It it is 4 kgs/hr, reaction terminates to stop charging and discharging in first 3 hours.Discharging enters another 50 liters continuous flowing agitator tank formulas
In reactor, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by 120000 milligrams of enzyme concentration/
Addition immobilised enzymes is risen, adjusts pH to 7.0, after being stirred 24 minutes by 200 revs/min, speed of agitator is adjusted to 150 revs/min,
Temperature control is at 70 DEG C；After reaction starting 3 hours, it is 4 kgs/hr to start continuous feed and discharging, input and output material speed, instead
It should terminate to stop charging and discharging in first 3 hours.The enzymolysis liquid of discharging is through centrifugation（12000 revs/min, 15 minutes, room temperature condition）
Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, 100 ls/h of evaporation capacity, temperature 70 C, pressure
0.30 MPa of power, cocnentration factor 1.8, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure, cross-flow speed
The meter per second of rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Sea-tangle residue and cake of camellia oleifera seeds, apple skin, lettuce
Lettuce waste dish, brewed spirit waste residue, chicken manure are with mass ratio 1:3:5:5:2:4 heap fermentations, 10 cubic metres of volume, 90 centimetres of height,
Temperature 70 C, temperature, fermentation time 20 ~ 40 days are controlled by translating into fermentation materials.It is organic that fermentation ends obtain high-quality solid-state
Fertilizer.
Embodiment 3.
Sea-tangle leftover pieces are cleaned with running water, then soaked 24 hours with running water, change water 1 time within during which every 3 hours；Immersion
Leftover pieces afterwards are shredded (wide with knife：0.5 cm, it is long：5 cms), broken sea-tangle crushes through colloid mill.Sea-tangle after crushing
Mud is with distilled water with volume ratio 1:5 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：50 MPas of pressure, is followed
Ring number 4 times, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：With steaming
Distilled water is rinsed 4 times, is placed in beaker, and pueraria root residue is with 25% Eusol with mass ratio 1:10 mix, and vibrate, press in shaking table
After 150 revs/min vibrate 20 minutes, adjustment of rotational speed is 100 revs/min, and temperature control duration of oscillation 36 hours, is shaken at 40 DEG C
Pueraria root residue distilled water flushing 4 times after terminating are swung, for immobilised enzymes.Cellulase immobilization method：Cellulase is dissolved in
0.25 mol/L phosphate buffer, pH of cushioning fluid 6.5, enzyme concentration are 25 g/l；Pueraria root residue and cellulose after pretreatment
Enzyme solutions are with mass ratio 1:10 mix, vibrated in shaking table, by 125 revs/min vibration 20 minutes after, adjustment of rotational speed be 75 turns/
Minute, temperature control at 30 DEG C, with distilled water rushed by duration of oscillation 36 hours, vibration secures cellulase pueraria root residue after terminating
Wash 4 times.The process for fixation of alkali protease is as follows：Alkali protease is dissolved in 0.25 mol/L phosphate buffer, buffer solution
PH value is 6.5, and enzyme concentration is 25 g/l；Pretreated pueraria root residue is with alkaline protease solution with mass ratio 1:10 mix,
Vibrated in shaking table, after being vibrated 20 minutes by 125 revs/min, adjustment of rotational speed is 75 revs/min, and temperature control is at 35 DEG C, vibration
Between 36 hours, vibration secures the pueraria root residue of alkali protease distilled water flushing 4 times after terminating.
Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:10 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to kelp residue and enzyme mass ratio 50:1
Add immobilised enzymes, adjust pH to 6.0,150 revs/min of speed of agitator, stirring 20 minutes after, speed of agitator be adjusted to 100 turns/
Minute, temperature control is at 55 DEG C；After immobilised enzymes Biocatalytic Conversion 2.5 hours, start continuous feed and discharging, input and output material speed
Rate is 2 kgs/hr, and reaction terminates to stop charging and discharging in first 2 hours.Discharging enters another 50 liters continuous flowing agitator tanks
In formula reactor, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by the milli of enzyme concentration 60000
G/l addition immobilised enzymes, adjust pH to 6.0, by 150 revs/min stirring 16 minutes after, speed of agitator is adjusted to 100 revs/min
Clock, temperature control is at 60 DEG C；After reaction starting 2.5 hours, starting continuous feed and discharging, input and output material speed is 2 kilograms/and it is small
When, reaction terminates to stop charging and discharging in first 2 hours.The enzymolysis liquid of discharging is through centrifugation（8000 revs/min, 10 minutes, room temperature bar
Part）Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, 60 ls/h of evaporation capacity, temperature 60 C,
0.15 MPa of pressure, cocnentration factor 1.5, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure, cross-flow
The meter per second of speed 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Sea-tangle residue and cake of camellia oleifera seeds, watermelon peel,
Romaine lettuce waste dish, brewed spirit waste residue, chicken manure are with mass ratio 1:2:4:4:2:3 heap fermentations, 7 cubic metres of volume, 70 centimetres of height,
Temperature 60 C, temperature, fermentation time 30 days are controlled by translating into fermentation materials.Fermentation ends obtain high-quality solid organic fertilizer.
Comparative example 1.
Sea-tangle leftover pieces are cleaned with running water, then soaked 24 hours with running water, change water 1 time within during which every 3 hours；Immersion
Leftover pieces afterwards are shredded (wide with knife：0.5 cm, it is long：5 cms).Broken sea-tangle is with distilled water with mass ratio 1:5 is mixed
It is even, it is placed in 50 liters of continuous flowing stirred tanks, does not add immobilised enzymes, adjusts pH to 5.5,100 turns of speed of agitator/
Minute, after stirring 10 minutes, speed of agitator is adjusted to 50 revs/min, and temperature control is at 45 DEG C；After reaction starting 2 hours, start
Continuous feed and discharging, input and output material speed are 0.5 kg/hr, and reaction terminates to stop charging and discharging in first 1 hour.Discharging
Into in another 50 liters continuous flowing stirred tanks, enzyme is not added, adjusts pH to 5.0,8 points are stirred by 100 revs/min
Zhong Hou, speed of agitator are adjusted to 50 revs/min, and temperature control is at 50 DEG C；After reaction starting 2 hours, start continuous feed and go out
Material, input and output material speed is 0.5 kg/hr, and reaction terminates to stop charging and discharging in first 1 hour.Out-feed liquid is through centrifugation（5000
Rev/min, 5 minutes, room temperature condition）Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, evaporation capacity
20 ls/h, temperature 50 C, 0.08 MPa of pressure, cocnentration factor 1.3, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature chamber
Temperature, 0.4 MPa of pressure, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract, sea-tangle residue afterwards
It is directly discarded.
Comparative example 2.
Sea-tangle leftover pieces are cleaned with running water, then soaked 24 hours with running water, change water 1 time within during which every 3 hours；Immersion
Leftover pieces afterwards are shredded (wide with knife：0.5 cm, it is long：5 cms), broken sea-tangle is with distilled water with mass ratio 1:20 is mixed
It is even, it is placed in 50 liters of continuous flowing stirred tanks, does not add enzyme, adjusts pH to 6.5,200 revs/min of speed of agitator,
After stirring 30 minutes, speed of agitator is adjusted to 150 revs/min, and temperature control is at 65 DEG C；After reaction starting 3 hours, start continuous
Charging and discharging, input and output material speed is 4 kgs/hr, and reaction terminates to stop charging and discharging in first 3 hours.Discharging enters another
In one continuous flowing stirred tank, enzyme is not added, adjusts pH to 7.0, after being stirred 24 minutes by 200 revs/min, stirring
Adjustment of rotational speed is 150 revs/min, and temperature control is at 70 DEG C；After reaction starting 3 hours, start continuous feed and discharging, input and output material
Speed is 4 kgs/hr, and reaction terminates to stop charging and discharging in first 3 hours.Out-feed liquid is through centrifugation（12000 revs/min, 15
Minute, room temperature condition）Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, 100 liters of evaporation capacity/small
When, temperature 70 C, 0.30 MPa of pressure, cocnentration factor 1.8, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, pressure
0.4 MPa, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Sea-tangle residue is directly useless
Abandon.
Comparative example 3.
Commercially available common foliar fertilizer.
Above example 1-3 and comparative example 1-3 application effect are as follows.
Crop field grape manure trial.
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather
Gently, sunshine is enriched, and makes a clear distinction between the four seasons, and summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-
18.6℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.80, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Kyoto grape.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 667 square metres.
Test and carried out in 2016, cell sets section ridging, vine 200 plants/acre of specification of plantation, 750 grams of Seedling Stage
Foliar fertilizer, 150 kilograms are watered, is stirred, foliage-spray is once；The Berry Growth phase, 1500 grams of foliar fertilizers, 150 kilograms are watered,
Foliage-spray stir once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate the disease incidences such as the big brown spot of grape, yellowtop, black rot, cracked fruit and weight method measure vintage,
Spectrophotometry grape soluble sugar content.
The alga fertilizer of table 1 handles grape effect
| Processing | Foliar fertilizer | Fertilization mode | The incidences of disease such as cracked fruit（%） | Vintage（Thousand grams/acre） | Soluble sugar content（Gram/100 grams） |
| 1 | Embodiment 1 | Each 1 time of seedling, Berry Growth | 5d | 2860a | 17.21c |
| 2 | Embodiment 2 | Each 1 time of seedling, Berry Growth | 6d | 2910a | 18.88b |
| 3 | Embodiment 3 | Each 1 time of seedling, Berry Growth | 4d | 2990a | 19.52a |
| 4 | Comparative example 1 | Each 1 time of seedling, Berry Growth | 38b | 2050c | 14.35e |
| 5 | Comparative example 2 | Each 1 time of seedling, Berry Growth | 43a | 2090c | 14.17e |
| 6 | Comparative example 3 | Each 1 time of seedling, Berry Growth | 32c | 2480b | 15.22d |
Field rice manure trial.
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather
Gently, sunshine is enriched, and makes a clear distinction between the four seasons, and summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-
18.6℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.60, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Y two excellent 1.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 2000 square metres.
Test and carried out in 2016, cell sets section ridging, conventional to plant specification, 1500 grams of foliar fertilizers of Seedling Stage, converts
150 kilograms of water, stirs, and foliage-spray is once；Ear growth period, 3000 grams of foliar fertilizers, be watered 150 kilograms, stir
Foliage-spray is once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate rice blast, the sheath and culm blight of rice, the sheath and culm blight of rice, etc. disease incidence and weight method measure rice yield.
The alga fertilizer of table 2 handles rice effect
| Processing | Foliar fertilizer | Fertilization mode | The incidences of disease such as rice blast（%） | Rice yield（Thousand grams/acre） |
| 1 | Embodiment 1 | Each 1 time of seedling, heading growth | 8c | 890a |
| 2 | Embodiment 2 | Each 1 time of seedling, heading growth | 9c | 860b |
| 3 | Embodiment 3 | Each 1 time of seedling, heading growth | 5c | 920a |
| 4 | Comparative example 1 | Each 1 time of seedling, heading growth | 22b | 580d |
| 5 | Comparative example 2 | Each 1 time of seedling, heading growth | 23b | 660c |
| 6 | Comparative example 3 | Each 1 time of seedling, heading growth | 25a | 750c |
Crop field tomato manure trial.
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather
Gently, sunshine is enriched, and makes a clear distinction between the four seasons, and summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-
18.6℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.80, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Rosy clouds powder tomato.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 667 square metres.
Test and carried out in 2016, cell sets section ridging, tomato 500 plants/acre of specification of plantation, 500 grams of Seedling Stage
Foliar fertilizer, 100 kilograms are watered, is stirred, foliage-spray is once；Bear fruit growth period, 1000 grams of foliar fertilizers, be watered 100 kilograms,
Foliage-spray stir once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate the disease incidences such as tomato droop, sclerotiniose, verticillium wilt and weight method measure tomato production.
The alga fertilizer of table 3 handles tomato effect
| Processing | Foliar fertilizer | Fertilization mode | The incidences of disease such as droop（%） | Tomato production（Thousand grams/acre） |
| 1 | Embodiment 1 | Each 1 time of seedling, Berry Growth | 6c | 4320a |
| 2 | Embodiment 2 | Each 1 time of seedling, Berry Growth | 7c | 4250a |
| 3 | Embodiment 3 | Each 1 time of seedling, Berry Growth | 4c | 4460a |
| 4 | Comparative example 1 | Each 1 time of seedling, Berry Growth | 28b | 3480c |
| 5 | Comparative example 2 | Each 1 time of seedling, Berry Growth | 30b | 3420c |
| 6 | Comparative example 3 | Each 1 time of seedling, Berry Growth | 35a | 3530b |
Different fertilization is can be seen that to the disease such as grape fruit split incidence of disease, vintage from table 1,2 and 3 result of the tests
Influence with soluble sugar content reaches significant difference level, and soluble sugar content height is related to grape quality.Soluble sugar
Content is higher, and grape quality is better.Different fertilization is to the disease such as the disease such as rice blast incidence of disease and tomato droop
The influence of the evil incidence of disease, rice and tomato production reaches significant difference level.Crop field grape, rice and tomato manure trial
It is all highest that the alga fertilizer that embodiment 2 is prepared, which applies rear grape, rice and tomato production, grape, rice and tomato
The incidence of disease is all minimum.It can be drawn from result of the test, alga fertilizer of the present invention can promote grape, rice and tomato growth,
Effectively preventing and treating grape, rice and tomato disease.
Claims (7)
- A kind of 1. preparation method of alga fertilizer, it is characterized in that according to the following steps：(1) sea-tangle waste pre-processes：Sea-tangle leftover pieces are cleaned with running water；Soaked 24 hours with running water again, it is during which every Change within 3 hours water 1 time；Leftover pieces after immersion are shredded with knife, and broken sea-tangle crushes through colloid mill；After crushing kelp paste and distilled water with Volume ratio 1:5 ~ 20 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：40 ~ 60 MPas of pressure, cycle-index 3 ~ 5 times, 1000 ls/h of flow, room temperature condition；(2) first step enzyme digestion reaction：Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:5 ~ 20 mix, and are placed in continuous stream In dynamic stirred tank, through pueraria root residue immobilization, immobilized cellulase is placed in be rotated cellulase together with agitating shaft Wire netting inframe, according to kelp residue and cellulase mass ratio 10 ~ 100:1 addition immobilized cellulase, adjusts pH to 5.5 ~ 6.5,100 ~ 200 revs/min of speed of agitator, after stirring 10 ~ 30 minutes, speed of agitator is adjusted to 50 ~ 150 revs/min, temperature Control is at 45 ~ 65 DEG C；After immobilized cellulase Biocatalytic Conversion 2 ~ 3 hours, start continuous feed and discharging, reaction terminate Stop charging and discharging within first 1 ~ 3 hour；Second step enzyme digestion reaction：Discharging enters in another continuous flowing stirred tank in step (2), alkali protease warp Pueraria root residue immobilization, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, dense by alkali protease Spend 300 ~ 12000 mg/litres addition immobilization alkali protease, adjust pH to 5.0 ~ 7.0, by 100 ~ 200 revs/min stirring 8 ~ After 24 minutes, speed of agitator is adjusted to 50 ~ 150 revs/min, and temperature control is at 50 ~ 70 DEG C；After reaction starting 2 ~ 3 hours, start Continuous feed and discharging, reaction terminate to stop charging and discharging in first 1 ~ 3 hour；(4) under the enzymolysis liquid room temperature condition that step (3) obtains, 5000 ~ 12000 revs/min, centrifuge 5 ~ 15 minutes, obtain supernatant Liquid and sea-tangle residue, supernatant falling film evaporation technology are concentrated, 20 ~ 100 ls/h of evaporation capacity, 50 ~ 70 DEG C of temperature, pressure 0.08 ~ 0.30 MPa, cocnentration factor 1.3 ~ 1.8, concentrate obtains liquid seaweed extract after ultrafiltration；(5) the sea-tangle residue in step (4) and cake of camellia oleifera seeds, pericarp, waste dish, brewage waste residue, chicken manure with mass ratio 1:1~3:3~ 5:3~5:1~2:1 ~ 4 heap fermentation, 4 ~ 10 cubic metres of volume, 50 ~ 90 centimetres of height, 50 ~ 70 DEG C of temperature, by translating into fermentate Expect to control temperature, fermentation time 20 ~ 40 days；Fermentation ends obtain high-quality solid organic fertilizer.
- 2. a kind of preparation method of alga fertilizer according to claim 1, it is characterized in that taken in step (2) continuous feed and Discharge method, speed are consistent, and are 0.5 ~ 4 kg/hr.
- A kind of 3. preparation method of alga fertilizer according to claim 1, it is characterized in that pueraria root residue is located in advance described in step (2) Reason method is：The distilled water flushing 3 ~ 5 times of pueraria root residue caused by kudzu-vine root powder production process, is placed in beaker, pueraria root residue with 10% ~ 40% Eusol is with mass ratio 1:5 ~ 15 mix, and are vibrated in shaking table, after being vibrated 10 ~ 30 minutes by 100 ~ 200 revs/min, Adjustment of rotational speed is 50 ~ 150 revs/min, and for temperature control at 30 ~ 50 DEG C, duration of oscillation 24 ~ 48 hours, vibration terminates rear pueraria root residue With distilled water flushing 3 ~ 5 times.
- A kind of 4. preparation method of alga fertilizer according to claim 1, it is characterized in that cellulase is consolidated described in step (2) Surely changing method is：Cellulase is dissolved in 0.05 ~ 0.5 mol/L phosphate buffer, and pH of cushioning fluid is 5.5 ~ 7.0, enzyme concentration For 5 ~ 50 g/l；Pueraria root residue and cellulase solution are with mass ratio 1 after pretreatment:5 ~ 20 mix, and are vibrated in shaking table, by 100 After ~ 150 revs/min of vibrations 10 ~ 30 minutes, adjustment of rotational speed is 50 ~ 100 revs/min, and temperature control is at 25 ~ 40 DEG C, duration of oscillation 24 ~ 48 hours, vibration secured the pueraria root residue of cellulase distilled water flushing 3 ~ 5 times after terminating.
- 5. a kind of preparation method of alga fertilizer according to claim 1, it is characterized in that taken in step (3) continuous feed and Discharge method, speed are consistent, and are 0.5 ~ 4 kg/hr.
- A kind of 6. preparation method of alga fertilizer according to claim 1, it is characterized in that the basic protein described in step (3) Enzyme immobilization method is as follows：Alkali protease is dissolved in 0.05 ~ 0.5 mol/L phosphate buffer, and pH of cushioning fluid is 5.5 ~ 7.0, enzyme concentration is 1 ~ 50 g/l；Pueraria root residue and alkaline protease solution are with mass ratio 1 after pretreatment:5 ~ 20 mix, in shaking table Middle vibration, after 100 ~ 150 revs/min of vibrations 10 ~ 30 minutes, adjustment of rotational speed is 50 ~ 100 revs/min, temperature control 25 ~ 50 DEG C, duration of oscillation 24 ~ 48 hours, vibration secures the pueraria root residue of alkali protease distilled water flushing 3 ~ 5 times after terminating.
- A kind of 7. preparation method of alga fertilizer according to claim 1, it is characterized in that the pericarp described in step (5) is perfume Any of several broadleaf plants skin, watermelon peel or apple skin, waste dish are Chinese cabbage, romaine lettuce or lettuce waste dish, and it is brewed spirit waste residue to brewage waste residue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710706522.7A CN107746296A (en) | 2017-08-17 | 2017-08-17 | A kind of preparation method of alga fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710706522.7A CN107746296A (en) | 2017-08-17 | 2017-08-17 | A kind of preparation method of alga fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107746296A true CN107746296A (en) | 2018-03-02 |
Family
ID=61254799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710706522.7A Pending CN107746296A (en) | 2017-08-17 | 2017-08-17 | A kind of preparation method of alga fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107746296A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108585979A (en) * | 2018-05-11 | 2018-09-28 | 天津市辰圣海洋生物技术有限公司 | It is a kind of using kelp as the alga fertilizer preparation method of raw material |
| CN109020666A (en) * | 2018-07-18 | 2018-12-18 | 威海市世代海洋生物科技股份有限公司 | Kelp digests liquid fertilizer and preparation method thereof, application method |
| CN112167268A (en) * | 2019-07-02 | 2021-01-05 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103627692A (en) * | 2013-11-13 | 2014-03-12 | 华南理工大学 | Method for immobilizing carbonic anhydrase by use of modified bagasse |
| CN104642530A (en) * | 2015-02-09 | 2015-05-27 | 福建农林大学 | Comprehensive utilization method for enteromorpha prolifera |
| CN104892157A (en) * | 2015-05-29 | 2015-09-09 | 领先生物农业股份有限公司 | Active seaweed fertilizer and production method thereof |
| CN106591381A (en) * | 2017-03-10 | 2017-04-26 | 淮海工学院 | Method for preparing bioethanol through continuous pretreatment of kelp residues |
| CN106915987A (en) * | 2017-03-28 | 2017-07-04 | 南昌大学 | A kind of preparation method of the special alga fertilizer of grape |
-
2017
- 2017-08-17 CN CN201710706522.7A patent/CN107746296A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103627692A (en) * | 2013-11-13 | 2014-03-12 | 华南理工大学 | Method for immobilizing carbonic anhydrase by use of modified bagasse |
| CN104642530A (en) * | 2015-02-09 | 2015-05-27 | 福建农林大学 | Comprehensive utilization method for enteromorpha prolifera |
| CN104892157A (en) * | 2015-05-29 | 2015-09-09 | 领先生物农业股份有限公司 | Active seaweed fertilizer and production method thereof |
| CN106591381A (en) * | 2017-03-10 | 2017-04-26 | 淮海工学院 | Method for preparing bioethanol through continuous pretreatment of kelp residues |
| CN106915987A (en) * | 2017-03-28 | 2017-07-04 | 南昌大学 | A kind of preparation method of the special alga fertilizer of grape |
Non-Patent Citations (1)
| Title |
|---|
| 史作清，施荣富主编: "《吸附分离树脂在医药工业中的应用》", 30 September 2008 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108585979A (en) * | 2018-05-11 | 2018-09-28 | 天津市辰圣海洋生物技术有限公司 | It is a kind of using kelp as the alga fertilizer preparation method of raw material |
| CN109020666A (en) * | 2018-07-18 | 2018-12-18 | 威海市世代海洋生物科技股份有限公司 | Kelp digests liquid fertilizer and preparation method thereof, application method |
| CN112167268A (en) * | 2019-07-02 | 2021-01-05 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
| CN112167268B (en) * | 2019-07-02 | 2021-12-10 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102515952B (en) | Chitosan coated alga bioorganic fertilizer and preparation method thereof | |
| CN106748195B (en) | The fertilizer and its preparation technology of a kind of growth substance containing natural plants | |
| CN107793214A (en) | A kind of alga fertilizer preparation method using sargassum fusifome as raw material | |
| CN101544523B (en) | Fertilizer with biological ferment and preparation method and use method thereof | |
| CN112167268B (en) | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract | |
| CN105036921A (en) | Plant nutrient solution and preparing method thereof | |
| CN109053299A (en) | A method of organic fertilizer is prepared using stalk and waste class organic waste | |
| CN106916021A (en) | A kind of double source humic acid biological fertilizer and preparation method and application | |
| CN104130068A (en) | Compound multifunctional biological foliage fertilizer | |
| CN107746296A (en) | A kind of preparation method of alga fertilizer | |
| CN103931463B (en) | Utilize the fruit tree integral fertilization method of waste bacterial slag | |
| CN1083408C (en) | Rapid fermenting process for preparing multi-element liquid fertilizer of amino acids | |
| CN109627099A (en) | A kind of spherical particle alkaline conditioner and its preparation | |
| CN104150977B (en) | Preparation method and application of alga oligosaccharide biological fertilizer | |
| CN107640982A (en) | A kind of alga fertilizer preparation method using yellow tang as raw material | |
| CN102153379A (en) | Method for producing liquid organic bacterial manure by utilizing waste liquor of restaurants and kitchens | |
| CN1562903A (en) | Quick acting compound mixed fertilizer of organic active liquor | |
| CN115215700B (en) | Liquid fertilizer based on biomass fermentation and preparation method thereof | |
| CN107540453A (en) | A kind of alga fertilizer preparation method using Enteromorpha as raw material | |
| CN107540455A (en) | A kind of alga fertilizer preparation method using sargassum as raw material | |
| CN107540454A (en) | A kind of alga fertilizer preparation method using sea-tangle as raw material | |
| CN106883051A (en) | A kind of insecticidal organic fertilizer and preparation method thereof | |
| CN106699445A (en) | Organic soil modifier and preparation method thereof | |
| CN1140482C (en) | Method for preparing resisting growth regulator of red alga polyphenols | |
| CN107540417A (en) | A kind of alga fertilizer preparation method using undaria pinnitafida as raw material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |