CN102153379A - Method for producing liquid organic bacterial manure by utilizing waste liquor of restaurants and kitchens - Google Patents
Method for producing liquid organic bacterial manure by utilizing waste liquor of restaurants and kitchens Download PDFInfo
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- CN102153379A CN102153379A CN2010105701480A CN201010570148A CN102153379A CN 102153379 A CN102153379 A CN 102153379A CN 2010105701480 A CN2010105701480 A CN 2010105701480A CN 201010570148 A CN201010570148 A CN 201010570148A CN 102153379 A CN102153379 A CN 102153379A
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Abstract
The invention relates to a method for producing liquid organic bacterial manure by utilizing the waste liquor of restaurants and kitchens. The method comprises the following steps of: selecting bacillus megaterium, candida lipolytica and pseudomonas; preparing a pseudomonas slope culture medium, a bacillus megaterium slope culture medium and a candida tropicalis slope culture medium; conveying the pseudomonas, the candida lipolytica and the bacillus megaterium into the fresh slope culture mediums respectively, and culturing at the temperature of 30 DEG C for 24 hours and activating; putting the activated strains into a culture medium in which agar is removed respectively, and culturing at the temperature of 30 DEG C for 18 hours at the rotation speed of 180 revolutions/minute of a shaker to obtain activated bacterial liquid, wherein the inoculation amount is 2 percent; inoculating the activated bacterial liquid of the pseudomonas, the bacillus megaterium and the candida lipolytica into the rubbish waste liquor of restaurants and kitchens in a ratio of 1:1:1, wherein the inoculation amount is 2 percent; adding 60 percent excrement, which serves as auxiliary materials, of livestock and poultry to prepare culture expanding bacterial liquid; and fermenting the compounded bacterial liquid at the temperature of 30 DEG C for 72 hours to obtain the liquid organic bacterial manure.
Description
Technical field
The present invention relates to a kind of waste resource regeneration field, particularly a kind of bacterial classification method that the utilization meal kitchen waste liquid of system produces liquid organic fungi-manure that spreads cultivation that comprises.
Background technology
Meal kitchen waste liquid COD reaches as high as more than the 100000mg/L, belongs to high concentrated organic wastewater, if enter water body, can cause serious eutrophication problem, and then cost is very high to handle qualified discharge.Therefore, utilize the meal kitchen waste liquid that is rich in organic composition to spread cultivation through microorganism and produce liquid bacterial manure, not only can eliminate discharge of wastewater, and realize the height recycling of waste liquid as nutritive medium.
Bacterial manure is being commonly called as of composite bio-fertilizer, is to adopt manual method to cultivate some beneficial microorganism and the bio-feritlizer made.This particular product that contains microorganism is applied in the agriculture production, can obtain specific fertilizer effect, and the vital movement of live microorganism plays a crucial role in the goods.Composite biological fertilizer contains beneficial microorganism more than two kinds, antagonism not between them, and can improve the level of supply of one or more nutritive elements of farm crop.Its fertilizer strengthens soil fertility to improving Soil structure, promotes plant growth, and aspects such as enhancing crop disease-resistant ability have vital role.After multiple functional microbial in the bio-bacterial manure enters soil, produce a large amount of secondary metabolites in the growth and breeding process, these products can promote the formation of soil aggregate.The formation of crumb structure makes the loose, soft of soil change, and the preserve moisture and fertility performance increases, and water, gas, heat are coordinated more, reduces soil compaction, helps water conservation, guarantor's fertilizer, ventilation and promotes root system development, for farm crop provide comfortable growing environment.The activity of soil beneficial microorganism has been strengthened in the improvement of soil physical and chemical property, thereby impels the organism decomposition and inversion to greatest extent, produce multiple nutrients material and pungent, impel microbial growth to grow, promote plant growth, finally reach the purpose of increasing both production and income.In addition, functional microbial forms dominant microflora around crop root, suppress or antagonism harmful microbe growth and breeding, has alleviated the degree of crop generation disease.
In the world, liquid fertilizer has obtained comparatively general application in some developed countries, occupies sizable ratio as the liquid fertilizer of states such as the U.S., Britain, Australia, Canada, Germany, Belgium, Holland in whole rate of fertilizer.China starts from the 1950's to the development research of liquid fertilizer, the seventies in Beijing, ground such as Zhejiang, Shandong, Hebei, Guangdong, Xinjiang carry out demonstration and popularization.The liquid fertilizer of domestic production is except that the big area root is used, a part is also as leaf dressing, execute irrigation as farm crop such as wheat, corn, cotton, paddy rice, beet, oily certain herbaceous plants with big flowers are carried out root with liquid fertilizer, and cash crop such as vegetables, fruit, gardens, flowers are often sprayed liquid fertilizer in vegetative period.The tobacco producing region also sprays in the phase or irrigation liquid fertilizer at tobacco growing except that bulk application.At present, the detail statistics data that China does not also have liquid fertilizer to use, according to a preliminary estimate, annual amount of application is about ten thousand tons of 30-40.
The characteristics of liquid fertilizer are that (1) productive expense is low.Chemical fertilizers production enterprise can save a part and produce necessary equipment of solid fertilizer or workshop section, as granulation, drying etc., helps reducing production equipment investment and maintenance cost, reduces the consumption of Ministry Of Fuel And Power power.(2) help environment protection.Liquid fertilizer does not have dust, non smoke in production and transportation, reduced because of " three wastes " discharging possibility pollution on the environment.Owing to removed equipment component, the sound pollution in the production process also can correspondingly reduce.(3) use conveniently.Liquid fertilizer can directly be manured into soil, and the foliage fertilizer that also can be used as various crops such as fruit tree, vegetables, flowers directly sprays, and can also combine sprinkling with agricultural chemicals.Because prescription flexibly, various nutritive elements are evenly reasonable, are applicable to make base manure, topdress.(4) directly by crop absorption.After liquid fertilizer was manured into soil, the nutritive ingredient root system that can go directly can reduce some nutritive ingredient by the quantity of soil fixing.When liquid fertilizer directly sprayed as foliage fertilizer, nutrition absorption was faster than soil application, can comparatively fast overcome farm crop nutrient deficiency symptom, can make plant absorb nutrition rapidly from the position, blade face.(5) reduce the fertilising cost.Because liquid fertilizer is used conveniently, helped by crop absorption, thereby can reduce the fertilizer application frequency to farm crop, save manpower expenditure and material resources consumption.Along with the paces that the large-scale production and the mechanize of liquid fertilizer are used are accelerated, the fertilising cost will further reduce.(6) liquid fertilizer also has certain limitation on using, and is higher relatively as trucking costs, is used for the special storage and the transportation equipment of liquid fertilizer needs of soil application, but also needs the special mean for applying of configuration etc.Constantly perfect along with the development of technology and auxiliary facility, these restraining factors will be cancelled gradually.Then there are not these problems at present in liquid compound fertilizer small-scale for some, special purpose.
In general, utilize meal kitchen waste liquid to produce liquid organic fungi-manure, both reduced pollution, save the wastewater treatment expense again, turn waste into wealth simultaneously, promote the related industries development, economic benefit and obvious environment benefit.
Summary of the invention
In order to solve sewage disposal and utilization of wastewater resource, realize the zero release problem that changing food waste is handled, fill up and utilize meal kitchen waste liquid to produce the blank of liquid bacterial manure technology, the invention provides a kind of method of utilizing meal kitchen waste liquid to produce liquid organic fungi-manure.The present invention realizes the sewage resource utilization, and the transformation efficiency height utilizes biotechnology, and energy consumption is very low, non-secondary pollution.
The present invention is by following technical scheme, realizes the method for utilizing meal kitchen waste liquid to produce liquid organic fungi-manure, the existing sewage resource utilization of this technology, and the transformation efficiency height utilizes the microorganism technology that spreads cultivation, and easy and simple to handle, energy consumption is very low, non-secondary pollution.
The method that utilization meal of the present invention kitchen waste liquid is produced liquid organic fungi-manure is as follows:
Entire method comprises following a few part: bacterial classification selection, breeding strain and activation, bacterium liquid are composite, and bacterial classification spreads cultivation and the preparation of liquid organic fungi-manure.Whole process of production does not have waste gas, waste liquid and waste residue to produce, and environmental risk is low.And organism utilizes again in the waste water, not only saves the wastewater treatment expense, and the creation of value, environmental benefit and remarkable in economical benefits.
(1) selection of bacterial classification
The used bacterial classification of the present invention is the production bacterial classification that the Ministry of Agriculture uses about regulation in microbial fertilizer Biosafety current techique criterion (NY11092006) appendix.Include but not limited to bacillus and yeast, said bacillus is bacillus megaterium or bacillus licheniformis or Bacillus subtillis, preferred Bacillus megatherium.Said yeast is or the saccharomyces lactis bacterium preferred Candida lipolytica.In addition, also comprise pseudomonas in the mixed bacterial of the present invention.
(2) breeding strain and activation method
The said method of breeding strain of the present invention is as follows:
1. pseudomonas slant medium preparation method (beef extract-peptone nutrient agar)
Extractum carnis 3g; Peptone 10g; NaCl 5g; Agar; Water 1000mL; PH 7.4-7.6
2. bacillus megaterium slant medium preparation method (phosphorus bacteria substratum)-
Glucose 10g; NaCI 0.3g; MgSO
40.3g; MnSO
40.03g; CaCO
31.0g; (NH
4)
2SO
40.5g; KCl 0.3g; Fe SO
40.03g; Yelkin TTS 0.2g; Yeast powder 0.5g; Agar; Water 1000mL; PH 7.0-7.2
3. candida tropicalis slant medium preparation method (bean sprouts medium)
Soybean sprout 100g; Glucose 20g; Water 1000mL; Agar; PH 7.2-7.4
Compound method is as follows: clean soybean sprout, add water boil 30min.Use filtered through gauze, add agar in the filtrate, put into sugar after the heating for dissolving, stir and make its dissolving, supply moisture to 1000mL, pH 7.2-7.4
4. shake-flask culture base: slant medium is removed agar as the shake-flask culture base
5. actication of culture and bacterium liquid and preparation method thereof: change pseudomonas, Candida lipolytica, bacillus megaterium over to fresh slant medium respectively, cultivate 24h, obtain the activatory bacterial classification at 30 ℃.The bacterial classification that activation is good is put into the shake-flask culture base respectively, and inoculum size is 1%～3%, and preferred 2%, all cultivated 10～30 hours down, preferably, all cultivated 18 hours at 30 ℃ at 20 ℃～40 ℃, shaking speed is 100 rev/mins～300 rev/mins, preferred 180 rev/mins.Bacterium liquid after obtaining to activate.
(3) mixed bacteria liquid preparation method
The pseudomonas that activation is good, bacillus megaterium, Candida lipolytica bacterium liquid are added in the changing food waste waste liquid with 1: 1: 1 ratio, and inoculum size is 1%～3%, and preferred 2%.Then, add the feces of livestock and poultry auxiliary material, add-on is 40%～80%, preferred 60%.
(4) bacterium liquid spreads cultivation and the organic fungi-manure preparation method
Composite good bacterium liquid ferments under 20 ℃～40 ℃, preferred 30 ℃.Fermentation time is 48 hours～96 hours, preferred 72 hours, promptly obtains liquid organic fungi-manure.
Embodiment
Select bacillus megaterium, Candida lipolytica and pseudomonas.Utilize extractum carnis 3g, peptone 10g, NaCI 5g, agar, water 1000mL, preparation pseudomonas slant medium; Utilize glucose 10g, NaCI0.3g, MgSO
40.3g, MnSO
40.03g, CaCO
31.0g, (NH
4)
2SO
40.5g, KCl 0.3g, Fe SO
40.03g, Yelkin TTS 0.2g, yeast powder 0.5g, agar, water 1000mL, preparation bacillus megaterium slant medium; Utilize soybean sprout 100g, glucose 20g, water 1000mL, agar, soybean sprout is cleaned, add water boil 30min.Use filtered through gauze, add agar in the filtrate, put into sugar after the heating for dissolving, stir and make its dissolving, supply moisture, preparation candida tropicalis slant medium to 1000mL.Change pseudomonas, Candida lipolytica, bacillus megaterium over to fresh slant medium respectively, cultivate 24h, obtain the activatory bacterial classification at 30 ℃.The bacterial classification that activation is good is put into the substratum of removing agar respectively, and inoculum size is 2%, all cultivates 18 hours at 30 ℃, and shaking speed is 180 rev/mins.Bacterium liquid after obtaining to activate.The pseudomonas that activation is good, bacillus megaterium, Candida lipolytica bacterium liquid are added in the changing food waste waste liquid with 1: 1: 1 ratio, and inoculum size is 2%.Then, add 60% feces of livestock and poultry auxiliary material, make the bacterium liquid that spreads cultivation.Composite good bacterium liquid promptly obtains liquid organic fungi-manure 30 ℃ of bottom fermentations 72 hours.Pseudomonas, bacillus megaterium, Candida lipolytica total viable count reach 10 in the tunning
8Individual/mL.In addition, the used bacterial classification of the present invention is the production bacterial classification that the Ministry of Agriculture uses about regulation in microbial fertilizer Biosafety current techique criterion (NY 11092006) appendix.
Claims (5)
1. method of utilizing meal kitchen waste liquid to produce liquid organic fungi-manure is characterized in that:
Entire method is including, but not limited to following several parts: bacterial classification selection, breeding strain and activation, bacterium liquid are composite, and bacterial classification spreads cultivation and the preparation of liquid organic fungi-manure.Whole process of production does not have waste gas, waste liquid and waste residue to produce, and environmental risk is low.And organism utilizes again in the waste water, not only saves the wastewater treatment expense, and the creation of value, environmental benefit and remarkable in economical benefits.
2. said bacterial classification includes but not limited to bacillus and yeast, and said bacillus is bacillus megaterium or bacillus licheniformis or Bacillus subtillis, preferred Bacillus megatherium.Said yeast is or the saccharomyces lactis bacterium preferred Candida lipolytica.In addition, also comprise pseudomonas in the said mixed bacterial.
3. said breeding strain and activation method are in the claim 1, utilize extractum carnis 3g, peptone 10g, NaCl 5g, agar, water 1000mL, preparation pseudomonas slant medium; Utilize glucose 10g, NaCI 0.3g, MgSO
40.3g, MnSO
40.03g, CaCO
31.0g, (NH
4)
2SO
40.5g, KCl 0.3g, Fe SO
40.03g, Yelkin TTS 0.2g, yeast powder 0.5g, agar, water 1000mL, preparation bacillus megaterium slant medium; Utilize soybean sprout 100g, glucose 20g, water 1000mL, agar, soybean sprout is cleaned, add water boil 30min, use filtered through gauze, add agar in the filtrate, put into sugar after the heating for dissolving, stirring makes its dissolving, supplies moisture to 1000mL, preparation candida tropicalis slant medium.Change the said bacterial classification of claim 2 over to fresh slant medium, cultivate 24h, obtain the activatory bacterial classification at 30 ℃.The bacterial classification that activation is good is put into the shake-flask culture base respectively, and inoculum size is 1%～3%, and preferred 2%, all cultivated 10～30 hours down, preferably, all cultivated 18 hours at 30 ℃ at 20 ℃～40 ℃, shaking speed is 100 rev/mins～300 rev/mins, preferred 180 rev/mins.Bacterium liquid after obtaining to activate.
4. said bacterium liquid preparation method is in the claim 1, and the pseudomonas that activation in the claim 3 is good, bacillus megaterium, Candida lipolytica bacterium liquid are added in the changing food waste waste liquid with 1: 1: 1 ratio, and inoculum size is 1%～3%, preferred 2%.Then, add the feces of livestock and poultry auxiliary material, add-on is 40%～80%, and is preferred 60%, makes the bacterium liquid that spreads cultivation.
5. the said bacterial classification of claim 1 spreads cultivation and the bacterial manure preparation method is, composite good bacterium liquid 30 ℃ of bottom fermentations 72 hours, is carried out bacterial classification and spreads cultivation, and promptly obtains liquid organic fungi-manure.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674960A (en) * | 2012-05-06 | 2012-09-19 | 冷登书 | Liquid fertilizer utilizing city life garbage landfill leachate and preparation method thereof |
| CN104839427A (en) * | 2015-05-15 | 2015-08-19 | 吴伟浩 | Method for preparing animal drinking water or feed mixing water from kitchen waste water |
| CN105950483A (en) * | 2016-07-15 | 2016-09-21 | 标优美生态工程股份有限公司 | Candida glabrata and application in kitchen waste |
| CN107056449A (en) * | 2017-04-19 | 2017-08-18 | 海南肥沃达生化科技有限公司 | Bacterial manure prepared with rubber plant's biogas slurry and preparation method thereof |
| CN109734486A (en) * | 2019-02-15 | 2019-05-10 | 陕西天仁雪农业科技有限公司 | A kind of kitchen garbage devil liquor recovery utilizes processing method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1548404A (en) * | 2003-05-13 | 2004-11-24 | 上海汇仁复合有机肥料有限公司 | Production process of microbial organic composite fertilizer |
| CN1903799A (en) * | 2006-08-11 | 2007-01-31 | 高鸿禄 | Comprehensive treatment technology of kitchen garbage |
| CN1911865A (en) * | 2005-08-12 | 2007-02-14 | 中国农业大学 | Method of producing active nutritional fertilizer using old domostic garbage |
| CN1974492A (en) * | 2006-12-11 | 2007-06-06 | 陈南岭 | Process of twice fermenting garbage-sludge mixture to produce microbial fertilizer |
| CN101073805A (en) * | 2007-07-16 | 2007-11-21 | 陈五岭 | Biological comprehensive treatment for food refuse |
| CN101709006A (en) * | 2009-11-16 | 2010-05-19 | 天津科技大学 | Method for treating solid organic wastes by biological method |
| CN101844940A (en) * | 2010-05-14 | 2010-09-29 | 张岳清 | Domestic organic food waste treatment method and treatment equipment |
-
2010
- 2010-12-02 CN CN2010105701480A patent/CN102153379A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1548404A (en) * | 2003-05-13 | 2004-11-24 | 上海汇仁复合有机肥料有限公司 | Production process of microbial organic composite fertilizer |
| CN1911865A (en) * | 2005-08-12 | 2007-02-14 | 中国农业大学 | Method of producing active nutritional fertilizer using old domostic garbage |
| CN1903799A (en) * | 2006-08-11 | 2007-01-31 | 高鸿禄 | Comprehensive treatment technology of kitchen garbage |
| CN1974492A (en) * | 2006-12-11 | 2007-06-06 | 陈南岭 | Process of twice fermenting garbage-sludge mixture to produce microbial fertilizer |
| CN101073805A (en) * | 2007-07-16 | 2007-11-21 | 陈五岭 | Biological comprehensive treatment for food refuse |
| CN101709006A (en) * | 2009-11-16 | 2010-05-19 | 天津科技大学 | Method for treating solid organic wastes by biological method |
| CN101844940A (en) * | 2010-05-14 | 2010-09-29 | 张岳清 | Domestic organic food waste treatment method and treatment equipment |
Non-Patent Citations (3)
| Title |
|---|
| 任连海等: "饮食业有机垃圾的产生现状及处理技术研究", 《北京工商大学学报(自然科学版)》 * |
| 吕凡等: "易腐性有机垃圾的产生与处理技术途径比较", 《环境污染治理技术与设备》 * |
| 龚仁等: "餐厨垃圾发酵生产生物活性蛋白饲料的工艺研究", 《饲料工业》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674960A (en) * | 2012-05-06 | 2012-09-19 | 冷登书 | Liquid fertilizer utilizing city life garbage landfill leachate and preparation method thereof |
| CN104839427A (en) * | 2015-05-15 | 2015-08-19 | 吴伟浩 | Method for preparing animal drinking water or feed mixing water from kitchen waste water |
| CN104839427B (en) * | 2015-05-15 | 2018-01-26 | 吴伟浩 | The method for being available for animal to drink moisturizing or feed water distribution is prepared with kitchen garbage, waste-water |
| CN105950483A (en) * | 2016-07-15 | 2016-09-21 | 标优美生态工程股份有限公司 | Candida glabrata and application in kitchen waste |
| CN105950483B (en) * | 2016-07-15 | 2020-01-03 | 标优美生态工程股份有限公司 | Candida glabrata and application thereof in kitchen waste |
| CN107056449A (en) * | 2017-04-19 | 2017-08-18 | 海南肥沃达生化科技有限公司 | Bacterial manure prepared with rubber plant's biogas slurry and preparation method thereof |
| CN109734486A (en) * | 2019-02-15 | 2019-05-10 | 陕西天仁雪农业科技有限公司 | A kind of kitchen garbage devil liquor recovery utilizes processing method |
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Application publication date: 20110817 |
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