CN106591381A - Method for preparing bioethanol through continuous pretreatment of kelp residues - Google Patents
Method for preparing bioethanol through continuous pretreatment of kelp residues Download PDFInfo
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- CN106591381A CN106591381A CN201710144234.7A CN201710144234A CN106591381A CN 106591381 A CN106591381 A CN 106591381A CN 201710144234 A CN201710144234 A CN 201710144234A CN 106591381 A CN106591381 A CN 106591381A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention provides a method for preparing bioethanol through continuous pretreatment of kelp residues. Kelp waste residues are taken as a raw material. The method comprises steps as follows: preliminary screening of the kelp residues, wetting, puffing, enzymolysis, preparation of an alcoholic fermentation culture medium, preparation of an ethanol fermenting liquid and preparation of bioethanol from the kelp residues and comprises specific steps as follows: wetting of the kelp residues in dilute acid, continuous puffing pretreatment, acquisition of an enzymatic saccharification solution after compound enzymolysis, yeast fermentation of the enzymatic saccharification solution, distillation of a fermentation liquid and obtaining of bioethanol. Through continuous puffing pretreatment of the kelp residues, the work cycle is shortened, the space structure of cellulose is effectively damaged, the cellulose crystallinity is reduced, the dosage of acid and enzymes is reduced, and the cost is saved; meanwhile, the method has the characteristic that the hydrolysis rate and the enzymolysis rate of cellulose of the kelp residues and the ethanol yield are increased, resource waste is reduced through recycling of kelp residue waste, environment pollution is avoided, and higher economic value is created.
Description
Technical field
The invention belongs to field of renewable energy, is soaked using diluted acid, extrusion pretreatment kelp residue carries out again complex enzyme hydrolysis
Fermentable sugars is prepared, bio-ethanol microbe conversion method is further carried out, more particularly to one kind is continuously pre-processed using kelp residue
The method for preparing bio-ethanol.
Background technology
With developing rapidly for society, chemical energy source is by substantial amounts of exploitation, day by day exhaustion.Biomass energy is used as change
The substitute for learning the energy is increasingly subject to pay attention to and develops, and wherein bio-ethanol is putative most promising renewable
Clean energy resource, its technology has obtained significant progress.First generation bio-ethanol is this mainly with starchiness and molasses class as raw material
The production technology of bio-ethanol comparative maturity.But can cause soil and potential as raw material large-scale production bio-ethanol
Food competition problem.Therefore, gradually it is taken seriously as the research of second generation bio-ethanol using cellulose resource.Cellulose
Resource conversion bio-ethanol is needed through certain pretreatment, and cellulose degraded and sweat could realize turning for ethanol
Change.Although step is bothered, cellulose is reproducible biomass resource the abundantest on the earth.The conversion of cellulose resource
Utilize, both mitigated the pollution that waste discharge is caused to ecological environment, energy demand is met again, be one and there is great potential
Frontier.Although the conversion process of second generation bio-ethanol has certain development, traditional terrestrial plant fiber is used as turning
Change raw material, but there is competitive relation with the resources of production such as soil and fresh water, its development nevertheless suffers from limiting.
Sea-tangle is the important kind of China's sea-farming, is one of primary raw material of brown alga industry.Define at present
Brown alga industrial products system with algin, mannitol, iodine as major product.But, on dry basis, the industrial utilization of sea-tangle
Rate is only 1/3, and the sea-tangle composition that there is also about 2/3 is not yet obtained by, and not only wastes substantial amounts of natural resources, but also brings
A series of problem of environmental pollutions.Containing about 50% crude fibre and hemicellulose in kelp residue, about 20% protein and big
General 3% ash content.Therefore kelp residue has the potential quality of transformation fermentation ethanol.
Kelp residue fermenting alcohol needs to be pre-processed, and current preconditioning technique has chemical method, Mechanical Method.The former has change
Learn amount of reagent big, process time is long, etching apparatus, pollute environment, need the specific conditions such as HTHP；Mechanical Crushing
Method, kelp residue cellulose crystallity is high, and not thoroughly, the usage amount of late enzyme is big, high cost for degraded.Therefore, above-mentioned single pre- place
Reason method has certain limitation and drawback.This method combines puffing technique using diluted acid wetting, makes hemicellulose degradation point
From cellulose crystallity is reduced, reduce sterically hindered, expose the binding site of enzyme molecule, improve saccharification yield and ethanol is produced
Rate.
The content of the invention
The shortcoming of prior art in view of the above, continuously pre-processes preparation and gives birth to the invention provides a kind of using kelp residue
The method of thing ethanol, with kelp residue as raw material, through the process of dilute sulfuric acid moistening and puffing, again the preparation of Jing complex enzyme zymohydrolysis can for the method
Sugar fermentation and yeast conversion production ethanol, not only by waste energy, while reducing the wasting of resources environmental pollution are avoided, and
With it is with short production cycle, cost-effective, degradation rate is high the features such as.
The method that a kind of continuous pretreatment of utilization kelp residue of the present invention prepares bio-ethanol, technical scheme is as follows：
The method that a kind of continuous pretreatment of utilization kelp residue prepares bio-ethanol, it is with Laminaria residue as raw material including following
Step：
(1) kelp residue initial screening：Kelp residue is collected and air-dried, using the broken kelp residue of hammer Universalpulverizer, 60-80 is crossed
Mesh sieve；
(2) kelp residue wetting：It is that 0.5% -1.5% (w/v) is dilute by the kelp residue concentration of screening in above-mentioned steps (1)
Sulfuric acid is soaked so as to water content to 30% -40%；
(3) kelp residue is expanded：Expanded place is carried out to the kelp residue in above-mentioned steps (2) using single screw squeezing expansion machine
Reason, crushed the mesh sieve of 60 mesh -80 standby by the kelp residue after expanding treatment；
(4) kelp residue enzymolysis：By the kelp residue of screening in above-mentioned steps (3), add after 10-15 times of water according to mass ratio,
Add calcium carbonate to adjust pH value to 5-5.5, add complex cellulase and protease to be stirred enzymolysis, the solution after enzymolysis enters
Row is processed and obtains kelp residue enzymatic saccharification liquid；
(5) preparation of alcohol fermentation culture medium：The kelp residue enzymatic saccharification liquid obtained in above-mentioned steps (4) is carried out dense
Contracting, is 25g/L -30g/L to content of reducing sugar, and in the saccharified liquid of concentration 0.15% dipotassium hydrogen phosphate and 0.25% sulphur are added
Sour ammonium, and pH value is adjusted to 5-5.5,115 DEG C of sterilizing 25min are obtained kelp residue saccharified liquid alcohol fermentation culture medium；
(6) preparation of alcohol fermentation liquid：Culture medium in above-mentioned steps (5) is accessed according to inoculum concentration 5% -10% pre-
The high activity saccharomyces cerevisiae for first activating and compounding and brewer's yeast, prepare alcohol fermentation liquid.
(7) preparation of ethanol：Zymotic fluid in above-mentioned steps (6) is carried out into distillation and prepares bio-ethanol.
Further, kelp residue dilute sulfuric acid 30-60min of wetting time.
Further, the single screw squeezing expansion machine rotating speed 500r/min, charging rate 20m/min -35m/min is swollen
Change 140 DEG C -180 DEG C of temperature, bulking pressure 1.5mPa -2.0mPa.
Further, the enzymolysis in the step (4), compound cellulose enzyme dosage be 15U/g -25U/g substrates, albumen
Enzyme dosage be 18U/g -25U/g substrates, hydrolysis temperature be 50 DEG C -55 DEG C, enzymolysis speed of agitator be 150r/min, enzymolysis time
For 18h -24h.
Further, the kelp residue enzymatic saccharification liquid in the step (4), the method using filtering to enzymolysis liquid or be centrifuged
Clear liquid is collected, to washing residue twice, water lotion merges with clear liquid and obtained.
Further, in the step (6) alcohol fermentation liquid preparation, wherein saccharomyces cerevisiae and brewer's yeast number be
108/ml, saccharomyces cerevisiae：Brewer's yeast consumption is 4:1,32 DEG C of culture 10h of Preliminary fermentation temperature, at interval of 2h in 150r/
Min shaking flasks 30min, 38-50h of quiescent culture after 10h.
Beneficial effects of the present invention are：
The present invention is raw material using Laminaria residue, by the expanded collaboration dilute acid pretreatment to kelp residue, is realized continuous
Change operation, shorten the work period, while reducing cellulose crystallity, peel off hemicellulose, be monose by its direct hydrolysis, have
Effect destroys the space structure of cellulose, improves follow-up enzymolysis efficiency.The present invention is by olefin(e) acid wetting, the hand of expanding treatment
Section, improves the percent hydrolysis of cellulose in kelp residue, reduces the consumption of acid, alkali, enzyme, cost-effective, while having reached acid
The effect that the solution time is short, accessory substance is few, improves the yield of fermentable sugars and ethanol.The utilization of kelp residue waste energy, subtracts
Lack the wasting of resources while avoiding environmental pollution, for resource reutilization an effective approach has been provided, with short production cycle,
The features such as cost-effective the characteristics of, raising kelp residue cellulose percent hydrolysis and alcohol yied, with very big development potentiality, sea-tangle
The reusing of energy source of slag, creates higher economic worth.
Description of the drawings
Fig. 1 kelp residues prepare the process flow diagram that sugar fermentation produces ethanol.
Specific embodiment
Following examples are described in detail to technical scheme, but the present invention is not restricted to lid and implements
Example.In order that the public has to the present invention thoroughly understand, in invention below preferred embodiment, be described in detail it is specific and
Details.
The present invention is elaborated below in conjunction with the accompanying drawings：As shown in figure 1, one kind continuously pre-processes preparation using kelp residue
The method of bio-ethanol, with Laminaria residue as raw material, comprises the following steps：
(1) kelp residue initial screening：Kelp residue is collected and air-dried, using the broken kelp residue of hammer Universalpulverizer, 60-80 is crossed
Mesh sieve；
(2) kelp residue wetting：It is that 0.5% -1.5% (w/v) is dilute by the kelp residue concentration of screening in above-mentioned steps (1)
Sulfuric acid is soaked so as to water content to 30% -40%；
(3) kelp residue is expanded：Expanded place is carried out to the kelp residue in above-mentioned steps (2) using single screw squeezing expansion machine
Reason, crushed the mesh sieve of 60 mesh -80 standby by the kelp residue after expanding treatment；
(4) kelp residue enzymolysis：By the kelp residue of screening in above-mentioned steps (3), add after 10-15 times of water according to mass ratio,
Add calcium carbonate to adjust pH value to 5-5.5, add complex cellulase and protease to be stirred enzymolysis, the solution after enzymolysis enters
Row is processed and obtains kelp residue enzymatic saccharification liquid；
(5) preparation of alcohol fermentation culture medium：The kelp residue enzymatic saccharification liquid obtained in above-mentioned steps (4) is carried out dense
Contracting, is 25g/L -30g/L to content of reducing sugar, and in the saccharified liquid of concentration 0.15% dipotassium hydrogen phosphate and 0.25% sulphur are added
Sour ammonium, and pH value is adjusted to 5-5.5,115 DEG C of sterilizing 25min are obtained kelp residue saccharified liquid alcohol fermentation culture medium；
(6) preparation of alcohol fermentation liquid：Culture medium in above-mentioned steps (5) is accessed according to inoculum concentration 5% -10% pre-
The high activity saccharomyces cerevisiae for first activating and compounding and brewer's yeast, prepare alcohol fermentation liquid.
(7) preparation of ethanol：Zymotic fluid in above-mentioned steps (6) is carried out into distillation and prepares bio-ethanol.
Kelp residue dilute sulfuric acid 30-60min of wetting time.
The single screw squeezing expansion machine rotating speed 500r/min, charging rate 20m/min -35m/min, swelling temperature 140
DEG C -180 DEG C, bulking pressure 1.5mPa -2.0mPa.
Enzymolysis in the step (4), compound cellulose enzyme dosage is 15U/g -25U/g substrates, and albumen enzyme dosage is
18U/g -25U/g substrates, hydrolysis temperature be 50 DEG C -55 DEG C, enzymolysis speed of agitator be 150r/min, enzymolysis time for 18h -
24h。
Kelp residue enzymatic saccharification liquid in the step (4), using the method for filtering to enzymolysis liquid or being centrifuged clear liquid is collected,
To washing residue twice, water lotion merges with clear liquid and is obtained.
The preparation of alcohol fermentation liquid in the step (6), wherein saccharomyces cerevisiae and brewer's yeast number is 108/ml, makes
Brewer yeast：Brewer's yeast consumption is 4:1,32 DEG C of culture 10h of Preliminary fermentation temperature, at interval of 2h in 150r/min shaking flasks 30min,
38-50h of quiescent culture after 10h.
Embodiment 1
(1) kelp residue is collected and air-dried, and crushes 80 mesh sieves using hammer Universalpulverizer standby；
(2) 10kg kelp residues are weighed 3kg, 0.5% (w/w) dilute sulfuric acid uniform wet 30min is used under stirring；
(3) kelp residue in above-mentioned steps (2) is carried out into expanding treatment, single screw rod extruding using single screw squeezing expansion machine
The rotating speed 500r/min of bulking machine, charging rate 20m/min, 120 DEG C of swelling temperature, bulking pressure 1.2mPa, it is expanded after sea
Band slag carries out crushing 80 mesh sieves standby；
(4) expanded rear kelp residue, adds 125L water stirring immersion 1h, then adjusts pH value to 5 with appropriate calcium carbonate, adds
Complex cellulase, protease that consumption be 20U/g substrate of the consumption for 20U/g substrates, under the conditions of 50 DEG C, 150r/min
Stirring enzymolysis 20h, enzymolysis liquid filters or is collected by centrifugation clear liquid, and a small amount of washing of residue twice, merges clear liquid, obtains kelp residue enzyme
Solution saccharified liquid.
(5) kelp residue saccharified liquid in above-mentioned steps (4) is concentrated into into reduced sugar 25g/L, adds 0.15% dipotassium hydrogen phosphate,
0.25% ammonium sulfate, adjusts pH value to 5.5,115 DEG C of sterilizing 25min, and kelp residue saccharified liquid fermenting alcohol culture medium is obtained.
(6) the fermenting alcohol culture medium obtained in above-mentioned steps (5) is accessed preactivated and is compounded by inoculum concentration 8%
Highly active number be that 108/ml saccharomyces cerevisiaes and number are in 108/ml brewer's yeasts, and saccharomyces cerevisiae and beer
Yeast amount ratio is 4：1, the fermented and cultured under the conditions of 32 DEG C initial 10 hours, was trained every 2 hours with 150r/min rotating speeds shaking flask
Foster 30min, after 10 hours quiescent culture 50 hours, and alcohol fermentation liquid is obtained.
(7) the alcohol fermentation liquid distillation obtained in above-mentioned steps (6) is obtained into kelp residue bio-ethanol, product quality is
1105.3g。
Embodiment 2
(1) kelp residue is collected and air-dried, and crushes 80 mesh sieves using hammer Universalpulverizer standby；
(2) 10kg kelp residues are weighed 4kg, 1% (w/w) dilute sulfuric acid uniform wet 50min is used under stirring；
(3) kelp residue in above-mentioned steps (2) is carried out into expanding treatment, single screw rod extruding using single screw squeezing expansion machine
The rotating speed 500r/min of bulking machine, charging rate 30m/min, 140 DEG C of swelling temperature, bulking pressure 1.6mPa, it is expanded after sea
Band slag carries out crushing 80 mesh sieves standby；
(4) expanded rear kelp residue, adds 125L water stirring immersion 1h, then adjusts pH value to 5 with appropriate calcium carbonate, adds
Complex cellulase, protease that consumption be 20U/g substrate of the consumption for 20U/g substrates, under the conditions of 50 DEG C, 150r/min
Stirring enzymolysis 20h, enzymolysis liquid filters or is collected by centrifugation clear liquid, and a small amount of washing of residue twice, merges clear liquid, obtains kelp residue enzyme
Solution saccharified liquid.
(5) kelp residue saccharified liquid in above-mentioned steps (4) is concentrated into into reduced sugar 25g/L, adds 0.15% dipotassium hydrogen phosphate,
0.25% ammonium sulfate, adjusts pH value to 5.5,115 DEG C of sterilizing 25min, and kelp residue saccharified liquid fermenting alcohol culture medium is obtained.
(6) the fermenting alcohol culture medium obtained in above-mentioned steps (5) is accessed preactivated and is compounded by inoculum concentration 8%
Highly active number be that 108/ml saccharomyces cerevisiaes and number are in 108/ml brewer's yeasts, and saccharomyces cerevisiae and beer
Yeast amount ratio is 4：1, the fermented and cultured under the conditions of 32 DEG C initial 10 hours, was trained every 2 hours with 150r/min rotating speeds shaking flask
Foster 30min, after 10 hours quiescent culture 50 hours, and alcohol fermentation liquid is obtained.
(7) the alcohol fermentation liquid distillation obtained in above-mentioned steps (6) is obtained into kelp residue bio-ethanol, product quality is
1205.6g。
Embodiment 3
(1) kelp residue is collected and air-dried, and crushes 80 mesh sieves using hammer Universalpulverizer standby；
(2) 10kg kelp residues are weighed 4kg, 1.5% (w/w) dilute sulfuric acid uniform wet 60min is used under stirring；
(3) kelp residue in above-mentioned steps (2) is carried out into expanding treatment, single screw rod extruding using single screw squeezing expansion machine
The rotating speed 500r/min of bulking machine, charging rate 35m/min, 150 DEG C of swelling temperature, bulking pressure 1.8mPa, it is expanded after sea
Band slag carries out crushing 80 mesh sieves standby；
(4) expanded rear kelp residue, adds 125L water stirring immersion 1h, then adjusts pH value to 5 with appropriate calcium carbonate, adds
Complex cellulase, protease that consumption be 20U/g substrate of the consumption for 20U/g substrates, under the conditions of 50 DEG C, 150r/min
Stirring enzymolysis 20h, enzymolysis liquid filters or is collected by centrifugation clear liquid, and a small amount of washing of residue twice, merges clear liquid, obtains kelp residue enzyme
Solution saccharified liquid.
(5) kelp residue saccharified liquid in above-mentioned steps (4) is concentrated into into reduced sugar 25g/L, adds 0.15% dipotassium hydrogen phosphate,
0.25% ammonium sulfate, adjusts pH value to 5.5,115 DEG C of sterilizing 25min, and kelp residue saccharified liquid fermenting alcohol culture medium is obtained.
(6) the fermenting alcohol culture medium obtained in above-mentioned steps (5) is accessed preactivated and is compounded by inoculum concentration 10%
Good highly active number is 108/ml saccharomyces cerevisiaes and number is in 108/ml brewer's yeasts, and saccharomyces cerevisiae and beer
Brewer yeast amount ratio is 4：1, the fermented and cultured under the conditions of 32 DEG C, initial 10 hours, every 2 hours with 150r/min rotating speed shaking flasks
Culture 30min, after 10 hours quiescent culture 50 hours, and alcohol fermentation liquid is obtained.
(7) the alcohol fermentation liquid distillation obtained in above-mentioned steps (6) is obtained into kelp residue bio-ethanol, product quality is
985.8g。
The above, only presently preferred embodiments of the present invention, above-described embodiment only principle of the illustrative present invention and
Its effect, and formal and substantial restriction not any to the present invention, it is noted that for the common skill of the art
Art personnel, on the premise of without departing from the inventive method, can also make some improvement and supplement, and these improve and supplement
Should be regarded as protection scope of the present invention.All those skilled in the art, in the feelings without departing from the spirit and scope of the present invention
Under condition, when a little change, modification made using disclosed above technology contents and the equivalent variations for developing, this is
The Equivalent embodiments of invention；Meanwhile, any equivalent variations that all substantial technologicals according to the present invention are made to above-described embodiment
Change, modify and develop, still fall within the range of technical scheme.
Claims (6)
1. the method that a kind of continuous pretreatment of utilization kelp residue prepares bio-ethanol, it is characterised in that：With Laminaria residue as raw material,
Comprise the following steps：
(1) kelp residue initial screening：Kelp residue is collected and air-dried, using the broken kelp residue of hammer Universalpulverizer, 60-80 mesh is crossed
Sieve；
(2) kelp residue wetting：It is 0.5% -1.5% (w/v) dilute sulfuric acid by the kelp residue concentration of screening in above-mentioned steps (1)
Wetting so as to water content to 30% -40%；
(3) kelp residue is expanded：Expanding treatment is carried out to the kelp residue in above-mentioned steps (2) using single screw squeezing expansion machine, will
It is standby that kelp residue after expanding treatment crushed the mesh sieve of 60 mesh -80；
(4) kelp residue enzymolysis：By the kelp residue of screening in above-mentioned steps (3), add after 10-15 times of water according to mass ratio, add
Calcium carbonate adjusts pH value to 5-5.5, adds complex cellulase and protease to be stirred enzymolysis, at the solution after enzymolysis
Reason obtains kelp residue enzymatic saccharification liquid；
(5) preparation of alcohol fermentation culture medium：The kelp residue enzymatic saccharification liquid obtained in above-mentioned steps (4) is concentrated, extremely
Content of reducing sugar is 25g/L -30g/L, and in the saccharified liquid of concentration 0.15% dipotassium hydrogen phosphate and 0.25% ammonium sulfate are added,
And pH value is adjusted to 5-5.5,115 DEG C of sterilizing 25min are obtained kelp residue saccharified liquid alcohol fermentation culture medium；
(6) preparation of alcohol fermentation liquid：Culture medium in above-mentioned steps (5) is accessed according to inoculum concentration 5% -10% living in advance
The high activity saccharomyces cerevisiae changed and compound and brewer's yeast, prepare alcohol fermentation liquid.
(7) preparation of ethanol：Zymotic fluid in above-mentioned steps (6) is carried out into distillation and prepares bio-ethanol.
2. the method that a kind of continuous pretreatment of utilization kelp residue according to claim 1 prepares bio-ethanol, its feature exists
In：Kelp residue dilute sulfuric acid 30-60min of wetting time.
3. the method that a kind of continuous pretreatment of utilization kelp residue according to claim 1 prepares bio-ethanol, its feature exists
In：The single screw squeezing expansion machine rotating speed 500r/min, charging rate 20m/min -35m/min, 140 DEG C of swelling temperature -
180 DEG C, bulking pressure 1.5mPa -2.0mPa.
4. the method that a kind of continuous pretreatment of utilization kelp residue according to claim 1 prepares bio-ethanol, its feature exists
In：Enzymolysis in the step (4), compound cellulose enzyme dosage be 15U/g -25U/g substrates, albumen enzyme dosage be 18U/g -
25U/g substrates, hydrolysis temperature is 50 DEG C -55 DEG C, and enzymolysis speed of agitator is 150r/min, and enzymolysis time is 18h -24h.
5. the method that a kind of continuous pretreatment of utilization kelp residue according to claim 1 prepares bio-ethanol, its feature exists
In：Kelp residue enzymatic saccharification liquid in the step (4), collects clear liquid, to residual using the method for filtering to enzymolysis liquid or being centrifuged
Pulp water is washed twice, and water lotion merges with clear liquid and obtained.
6. the method that a kind of continuous pretreatment of utilization kelp residue according to claim 1 prepares bio-ethanol, its feature exists
In：The preparation of alcohol fermentation liquid in the step (6), wherein saccharomyces cerevisiae and brewer's yeast number are 108/ml, ferment of making wine
It is female：Brewer's yeast consumption is 4:1,32 DEG C of culture 10h of Preliminary fermentation temperature, at interval of 2h in 150r/min shaking flasks 30min, 10h
38-50h of quiescent culture afterwards.
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