CN103923949A - Method and apparatus for producing ethanol through synchronous saccharification and fermentation of lignocellulose - Google Patents
Method and apparatus for producing ethanol through synchronous saccharification and fermentation of lignocellulose Download PDFInfo
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- CN103923949A CN103923949A CN201410093862.3A CN201410093862A CN103923949A CN 103923949 A CN103923949 A CN 103923949A CN 201410093862 A CN201410093862 A CN 201410093862A CN 103923949 A CN103923949 A CN 103923949A
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Abstract
The invention provides a method for producing ethanol through synchronous saccharification and fermentation of lignocellulose. The method comprises the following steps: carrying out saccharification on lignocellulose in a saccharification kettle by using cellulase; collecting a saccharification liquid obtained after enzymatic hydrolysis; and carrying out fermentation on the saccharification liquid in a fermentation kettle by using a yeast culture so as to obtain a fermentation broth containing ethanol. The method is characterized in that a part of the saccharification liquid is collected from the saccharification kettle and conveyed to a filtering device during saccharification, a solid phase obtained after filtration is returned to the saccharification kettle, a liquid phase obtained after filtration is delivered to the fermentation kettle, and a same volume of the fermentation broth is cycled and returned to the saccharification kettle. The invention further provides an apparatus for producing ethanol through synchronous saccharification and fermentation of lignocellulose. According to the invention, an inhibiting effect on cellulase caused by accumulation of glucose in hydrolysate during step-by-step saccharification and fermentation is avoided, the usage amount of enzyme is reduced, and cost is lowered down.
Description
Technical field
The technology of the present invention belongs to biomass energy and resource technical field, particularly relates to a kind of method that lignocellulose simultaneous saccharification and fermentation is produced ethanol.
Background technology
Along with exhaustion increasingly and the environmental pollution of oil and coal resource are day by day serious, utilize renewable energy source to become further important as the substitute of fossil energy.And alcohol fuel is the principal mode of biomass energy, it is also the most feasible substitute of fossil energy.
The main technique route of preparing alcohol fuel is: the pre-treatment of raw material, saccharification, fermentation, ethanol purification dehydration.Wherein saccharification, fermentation can proceed step by step, also can synchronously carry out.
Simultaneous saccharification and fermentation remains in lower scope the glucose concn in enzymolysis solution, can not produce feedback inhibition to cellulase, has improved saccharification efficiency, has reduced the consumption of cellulase, and required conversion unit reduces relatively.But simultaneous saccharification and fermentation can not be worked cellulase and yeast simultaneously at suitable separately temperature, and yeast, owing to mixing with not saccharification biomass of part, cannot improve fermentation efficiency by circulation in addition.
Substep diastatic fermentation refers to first uses cellulase hydrolysis Mierocrystalline cellulose, then collect the liquid glucose after enzymolysis, recycling yeast fermentation is produced ethanol, the advantage of this method all can be controlled under optimum condition cellulase and yeast to carry out, the glucose producing when shortcoming is cellulose hydrolysis is lived the enzyme that suppresses cellulase, cellulase consumption is increased, enzymolysis efficiency reduces, while needing to be hydrolyzed, substrate concn is lower simultaneously, cause lower ethanol production, thereby increased fermentation costs and ethanol cost recovery.
Need at present a kind of technology can overcome the shortcoming of substep diastatic fermentation technology, the glucose producing while solving cellulose hydrolysis suppresses the enzyme of cellulase lives, and cellulase consumption is increased, the problem that enzymolysis efficiency reduces.
Summary of the invention
The present invention is directed to the downtrod problem of lignocellulose saccharifying in existing substep diastatic fermentation technology, a kind of lignocellulose simultaneous saccharification and fermentation of proposition is produced the method for ethanol, comprises the steps:
(1) raw material hot high pressure water law pre-treatment: under normal temperature, stalk is dried, be ground to after certain particle size dryly, under suitable solid-to-liquid ratio, mix with hot water, then under certain temperature and pressure, stir after the regular hour, solid-liquid separation, solid phase is dried to constant weight.
(2) simultaneous saccharification and fermentation: gained solid is through high-temp steam sterilizing; Solid after sterilizing, cellulase and sterilized water are mixed, be dosed to saccharification still, carry out under certain conditions saccharification, partially saccharifying liquid is delivered to membrane separation unit by impeller pump simultaneously, solid phase (be mainly not completely the lignocellulose of saccharification) is back to saccharification still after filtering, the clear liquor that is rich in monose is delivered to the fermentation cauldron containing thermotolerant yeast bacterium culture, for keeping saccharification still and fermentation cauldron internal volume constant, the fermentation liquid circulation of same volume is returned to saccharification still simultaneously.After completion of the reaction, carry out solid-liquid separation, gained liquid is the fermented liq that is rich in ethanol.Synchronous saccharification filters fermentation and is comprised of saccharification, filtration, fermentation three parts.
In preprocessing process, the preferred maize straw of stalk, rice straw, broomcorn straw or wheat stalk; The grind size of stalk is 40～80 orders preferably; Preferred 1:5～the 1:15 of mass ratio of stalk particle and hot water; Preferably 150～200 ℃ of hot water temperatures; Pretreated working pressure is 1～5MPa preferably; The pretreated time is 10～60min preferably.
In simultaneous saccharification and fermentation process, the preferred 1g:10FPU-30FPU:5-10g of the ratio of the solid after sterilizing, cellulase and sterilized water.40～60 ℃ of saccharification condition optimizations, pH=4～6.Separatory membrane aperture is 0.2 μ m preferably, the preferred cross-flow of filter type.The preferred agar of Yeast culture, peptone and yeast powder, wherein agar concentration is 15～25g/L, and peptone concentration is 10～20g/L, and yeast powder concentration is 5～15g/L.Preferably 20～40 ℃ of fermentation conditions, pH=4～6.Synchronous saccharification filters preferably 60～120h of fermentation time.
The present invention also provides a kind of lignocellulose simultaneous saccharification and fermentation to produce the device of ethanol, comprises successively: for making the saccharification still of lignocellulose saccharification; For extract the impeller pump of part saccharified liquid out from saccharification still; The filtration unit filtering for the saccharified liquid that impeller pump is transported; For extract the impeller pump of liquid phase out from filtration unit; The fermentation cauldron fermenting for the liquid phase that filtration unit is extracted out; And for the fermentation liquid circulation of same volume being back to the impeller pump of saccharification still.
In this device, filtration unit is membrane separation unit, and the aperture of separatory membrane is 0.2 μ m.
The present invention brings following technique effect:
(1) the present invention all can be controlled under optimum condition cellulase and yeast to carry out, greatly improved saccharification and fermentation efficiency, reduced the consumption of cellulase and yeast, reduced cost.
(2) the present invention is delivered to ferment tank by the hydrolyzed solution of part clarification, has avoided in substep diastatic fermentation process, and due to the accumulation of glucose in hydrolyzed solution, the restraining effect that cellulase is produced, has reduced enzyme dosage, has reduced cost.
(3) the present invention is by membrane separation unit, avoided in traditional synchronous fermentation saccharifying yeast owing to mixing with not saccharification biomass of part the disadvantageous effect that reduces fermentation efficiency.
Accompanying drawing explanation
Fig. 1 shows technical process of the present invention.
Embodiment
Below by specific embodiment, the invention will be further described.
The following examples are in order to enable those skilled in the art to better understand the present invention, but the present invention are not imposed any restrictions.
Embodiment 1
A method that improves simultaneous saccharification and fermentation efficiency in lignocellulose ethanol process processed, comprises the steps:
Under normal temperature, maize straw is dried, be then ground to 60 orders, after stalk particle after grinding is dry and hot water by the mass ratio of 1:10, put in pre-press tank, in temperature, be 170 ℃, under the condition that pressure is 2MPa, stir after 30min cooling, solid-liquid separation, solid phase is dried to constant weight.Gained solid is through high-temp steam sterilizing, solid after sterilizing, cellulase and sterilized water are mixed with the ratio of 1g:20FPU:8g, be dosed to saccharification still, saccharification temperature is 50 ℃, pH=5, simultaneously partially saccharifying liquid is delivered to membrane separation unit by impeller pump, and solid phase (be mainly not completely the lignocellulose of saccharification) is back to saccharification still after filtering, and the clear liquor that is rich in monose is delivered to the fermentation cauldron fermentation containing thermotolerant yeast bacterium culture, leavening temperature is 30 ℃, pH=5.Meanwhile, for keeping saccharification still and fermentation cauldron internal volume constant, the fermentation liquid circulation of same volume is returned to saccharification still simultaneously.Whole process carries out, after 96h, carrying out solid-liquid separation, and gained liquid is the fermented liq that is rich in ethanol, and wherein ethanol content is 51.3g/L, is 80.1% of theoretical value.
Embodiment 2
A method that improves simultaneous saccharification and fermentation efficiency in lignocellulose ethanol process processed, comprises the steps:
Under normal temperature, rice straw is dried, be then ground to 50 orders, after stalk particle after grinding is dry and hot water by the mass ratio of 1:12, put in pre-press tank, in temperature, be 160 ℃, under the condition that pressure is 1.5MPa, stir after 40min cooling, solid-liquid separation, solid phase is dried to constant weight.Gained solid is through high-temp steam sterilizing, solid after sterilizing, cellulase and sterilized water are mixed with the ratio of 1g:18FPU:10g, be dosed to saccharification still, saccharification temperature is 50 ℃, pH=5, simultaneously partially saccharifying liquid is delivered to membrane separation unit by impeller pump, and solid phase (be mainly not completely the lignocellulose of saccharification) is back to saccharification still after filtering, and the clear liquor that is rich in monose is delivered to the fermentation cauldron fermentation containing thermotolerant yeast bacterium culture, leavening temperature is 30 ℃, pH=5.Meanwhile, for keeping saccharification still and fermentation cauldron internal volume constant, the fermentation liquid circulation of same volume is returned to saccharification still simultaneously.Whole process carries out, after 96h, carrying out solid-liquid separation, and gained liquid is the fermented liq that is rich in ethanol, and wherein ethanol content is 55.6g/L, is 83.2% of theoretical value.
Embodiment 3
A method that improves simultaneous saccharification and fermentation efficiency in lignocellulose ethanol process processed, comprises the steps:
Under normal temperature, wheat stalk is dried, be then ground to 40 orders, after stalk particle after grinding is dry and hot water by the mass ratio of 1:10, put in pre-press tank, in temperature, be 150 ℃, under the condition that pressure is 3MPa, stir after 30min cooling, solid-liquid separation, solid phase is dried to constant weight.Gained solid is through high-temp steam sterilizing, solid after sterilizing, cellulase and sterilized water are mixed with the ratio of 1g:20FPU:8g, be dosed to saccharification still, saccharification temperature is 50 ℃, pH=5, simultaneously partially saccharifying liquid is delivered to membrane separation unit by impeller pump, and solid phase (be mainly not completely the lignocellulose of saccharification) is back to saccharification still after filtering, and the clear liquor that is rich in monose is delivered to the fermentation cauldron fermentation containing thermotolerant yeast bacterium culture, leavening temperature is 30 ℃, pH=5.Meanwhile, for keeping saccharification still and fermentation cauldron internal volume constant, the fermentation liquid circulation of same volume is returned to saccharification still simultaneously.Whole process carries out, after 96h, carrying out solid-liquid separation, and gained liquid is the fermented liq that is rich in ethanol, and wherein ethanol content is 52.7g/L, is 81.2% of theoretical value.
Embodiment 4
A method that improves simultaneous saccharification and fermentation efficiency in lignocellulose ethanol process processed, comprises the steps:
Under normal temperature, broomcorn straw is dried, be then ground to 60 orders, after stalk particle after grinding is dry and hot water by the mass ratio of 1:12, put in pre-press tank, in temperature, be 160 ℃, under the condition that pressure is 2.5MPa, stir after 30min cooling, solid-liquid separation, solid phase is dried to constant weight.Gained solid is through high-temp steam sterilizing, solid after sterilizing, cellulase and sterilized water are mixed with the ratio of 1g:20FPU:10g, be dosed to saccharification still, saccharification temperature is 50 ℃, pH=5, simultaneously partially saccharifying liquid is delivered to membrane separation unit by impeller pump, and solid phase (be mainly not completely the lignocellulose of saccharification) is back to saccharification still after filtering, and the clear liquor that is rich in monose is delivered to the fermentation cauldron fermentation containing thermotolerant yeast bacterium culture, leavening temperature is 30 ℃, pH=5.Meanwhile, for keeping saccharification still and fermentation cauldron internal volume constant, the fermentation liquid circulation of same volume is returned to saccharification still simultaneously.Whole process carries out, after 96h, carrying out solid-liquid separation, and gained liquid is the fermented liq that is rich in ethanol, and wherein ethanol content is 53.3g/L, is 83.1% of theoretical value.
Although illustrated and described embodiments of the invention, for the ordinary skill in the art, be appreciated that without departing from the principles and spirit of the present invention and can carry out multiple variation, modification, replacement and modification to these embodiment, scope of the present invention is limited by claims and equivalent thereof.
Claims (11)
1. lignocellulose simultaneous saccharification and fermentation is produced a method for ethanol, comprising: in saccharification still, utilize cellulase to carry out saccharification to lignocellulose; Collect the saccharified liquid after enzymolysis; And in fermentation cauldron, utilize Yeast culture to ferment and obtain the fermented liquid that contains ethanol saccharified liquid; It is characterized in that: in the time of saccharification, from saccharification still, collect part saccharified liquid and be delivered to filtration unit, solid phase is after filtering back to saccharification still, and liquid phase is after filtering delivered to fermentation cauldron, the fermentation liquid circulation of same volume is back to saccharification still simultaneously.
2. method according to claim 1, is characterized in that: described filtration unit is membrane separation unit, and the aperture of separatory membrane is 0.2 μ m.
3. method according to claim 2, is characterized in that: filter type is cross-flow.
4. according to the method described in any one in claim 1-3, it is characterized in that: described lignocellulose is from one or more in maize straw, rice straw, broomcorn straw or wheat stalk.
5. method according to claim 4, it is characterized in that: at normal temperatures, stalk is dried, after being ground to 40～80 object granularities, be dried, under the solid-liquid mass ratio of 1:5～1:15, mix with hot water, then solid-liquid separation after stirring 10～60min under the pressure of the temperature of 150～200 ℃ and 1～5MPa, solid phase is dried to constant weight and is obtained described lignocellulose.
6. according to the method described in any one in claim 1-3, it is characterized in that: the temperature of saccharification is 40～60 ℃, pH=4～6.
7. according to the method described in any one in claim 1-3, it is characterized in that: before saccharification, lignocellulose process high-temperature sterilization, and mix with cellulase and sterilized water, the blending ratio of the lignocellulose after sterilizing, cellulase and sterilized water is 1g:10FPU-30FPU:5-10g.
8. according to the method described in any one in claim 1-3, it is characterized in that: described Yeast culture is agar, peptone and yeast powder, wherein agar concentration is 15～25g/L, and peptone concentration is 10～20g/L, and yeast powder concentration is 5～15g/L.
9. according to the method described in any one in claim 1-3, it is characterized in that: the temperature of fermentation is 20～40 ℃, pH=4～6.
10. lignocellulose simultaneous saccharification and fermentation is produced a device for ethanol, comprises successively: for making the saccharification still of lignocellulose saccharification; For extract the impeller pump of part saccharified liquid out from saccharification still; The filtration unit filtering for the saccharified liquid that impeller pump is transported; For extract the impeller pump of liquid phase out from filtration unit; The fermentation cauldron fermenting for the liquid phase that filtration unit is extracted out; And for the fermentation liquid circulation of same volume being back to the impeller pump of saccharification still.
11. devices according to claim 10, wherein, described filtration unit is membrane separation unit, and the aperture of separatory membrane is 0.2 μ m.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520845A (en) * | 2015-09-15 | 2017-03-22 | 中国科学院大连化学物理研究所 | Lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology |
| CN107118990A (en) * | 2017-05-22 | 2017-09-01 | 中国科学院成都生物研究所 | Its production γ PGA of high yield γ PGA bacterial strains and application method |
| CN107746867A (en) * | 2017-10-31 | 2018-03-02 | 无锡甜丰食品有限公司 | A kind of purification process of malt syrup |
| CN108101645A (en) * | 2017-12-29 | 2018-06-01 | 中溶科技股份有限公司 | A kind of method using biogas residue production bio-feritlizer |
| CN110055160A (en) * | 2019-04-28 | 2019-07-26 | 北京师范大学 | Continuous cascaded immobilized reactant method and apparatus for cellulose alcoholic fermentation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760482A (en) * | 2008-12-24 | 2010-06-30 | 安琪酵母股份有限公司 | Production method of cellulose ethanol |
| CN102174593A (en) * | 2011-03-08 | 2011-09-07 | 中国科学院过程工程研究所 | Process for producing high-concentration ethanol by coupling lignocellulose fermentation and membrane separation |
| CN103074385A (en) * | 2013-01-29 | 2013-05-01 | 大连工业大学 | Method for preparing ethanol with lignocellulose through fed-batch semi-simultaneous saccharification and fermentation |
-
2014
- 2014-03-13 CN CN201410093862.3A patent/CN103923949A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760482A (en) * | 2008-12-24 | 2010-06-30 | 安琪酵母股份有限公司 | Production method of cellulose ethanol |
| CN102174593A (en) * | 2011-03-08 | 2011-09-07 | 中国科学院过程工程研究所 | Process for producing high-concentration ethanol by coupling lignocellulose fermentation and membrane separation |
| CN103074385A (en) * | 2013-01-29 | 2013-05-01 | 大连工业大学 | Method for preparing ethanol with lignocellulose through fed-batch semi-simultaneous saccharification and fermentation |
Non-Patent Citations (2)
| Title |
|---|
| 杨富国等: "膜分离技术在低聚木糖制备及乙醇发酵中的应用", 《林产化学与工业》 * |
| 石贵阳等: "外环流式发酵罐用于玉米生料酒精发酵的研究", 《西北农林科技大学学报(自然科学版)》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520845A (en) * | 2015-09-15 | 2017-03-22 | 中国科学院大连化学物理研究所 | Lignocellulose simultaneous saccharification and fermentation process surfactant recovery technology |
| CN106520845B (en) * | 2015-09-15 | 2020-05-08 | 中国科学院大连化学物理研究所 | Surfactant recovery technology in lignocellulose synchronous saccharification and fermentation process |
| CN107118990A (en) * | 2017-05-22 | 2017-09-01 | 中国科学院成都生物研究所 | Its production γ PGA of high yield γ PGA bacterial strains and application method |
| CN107118990B (en) * | 2017-05-22 | 2020-03-27 | 中国科学院成都生物研究所 | High-yield gamma-PGA strain and method for producing gamma-PGA by using same |
| CN107746867A (en) * | 2017-10-31 | 2018-03-02 | 无锡甜丰食品有限公司 | A kind of purification process of malt syrup |
| CN108101645A (en) * | 2017-12-29 | 2018-06-01 | 中溶科技股份有限公司 | A kind of method using biogas residue production bio-feritlizer |
| CN110055160A (en) * | 2019-04-28 | 2019-07-26 | 北京师范大学 | Continuous cascaded immobilized reactant method and apparatus for cellulose alcoholic fermentation |
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