CN111440839A - Preparation method of cellulosic ethanol - Google Patents
Preparation method of cellulosic ethanol Download PDFInfo
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- CN111440839A CN111440839A CN202010452186.XA CN202010452186A CN111440839A CN 111440839 A CN111440839 A CN 111440839A CN 202010452186 A CN202010452186 A CN 202010452186A CN 111440839 A CN111440839 A CN 111440839A
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 187
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 238000000855 fermentation Methods 0.000 claims abstract description 65
- 239000010902 straw Substances 0.000 claims abstract description 35
- 238000004519 manufacturing process Methods 0.000 claims abstract description 27
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 24
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 24
- 239000001913 cellulose Substances 0.000 claims abstract description 24
- 229920002678 cellulose Polymers 0.000 claims abstract description 24
- 239000002994 raw material Substances 0.000 claims abstract description 19
- 239000002154 agricultural waste Substances 0.000 claims abstract description 18
- 241000186146 Brevibacterium Species 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 230000000593 degrading effect Effects 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims description 63
- 239000000843 powder Substances 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 19
- 238000011081 inoculation Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 15
- 239000001963 growth medium Substances 0.000 claims description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 240000008042 Zea mays Species 0.000 claims description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 8
- 235000005822 corn Nutrition 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000012137 tryptone Substances 0.000 claims description 6
- 238000003988 headspace gas chromatography Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 244000098338 Triticum aestivum Species 0.000 claims description 3
- 244000038559 crop plants Species 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000003912 environmental pollution Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000010565 inoculated fermentation Methods 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 2
- 244000105624 Arachis hypogaea Species 0.000 claims description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 2
- 235000018262 Arachis monticola Nutrition 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 240000006394 Sorghum bicolor Species 0.000 claims description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 2
- 235000020232 peanut Nutrition 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 239000002699 waste material Substances 0.000 abstract description 4
- 238000009776 industrial production Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 241000793189 Saccharomyces cerevisiae BY4741 Species 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 235000019441 ethanol Nutrition 0.000 description 50
- 235000013339 cereals Nutrition 0.000 description 6
- 239000006227 byproduct Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000002803 fossil fuel Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 241000609240 Ambelania acida Species 0.000 description 1
- 244000226021 Anacardium occidentale Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101710112457 Exoglucanase Proteins 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 235000021310 complex sugar Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000013587 production medium Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention provides a preparation method of cellulose ethanol, which belongs to the technical field of biology, and has the advantages that the principle is clear, the effect of degrading cellulose to form ethanol through co-fermentation of brevibacterium frigostolerant and saccharomyces cerevisiae is obvious, and the cellulose can be effectively decomposed to generate ethanol; the production raw materials used BY the invention are agricultural wastes, the strains are screened strains of brevibacterium frigosum and preserved strains of saccharomyces cerevisiae BY4741, the preparation process of the cellulosic ethanol is simple, the cost is low, no secondary pollution is caused to the environment, the crop straws can be degraded BY the cellulose decomposing bacteria, the required energy substances can be obtained, the demand on energy is low in the reaction process, the energy is less lost, the economic value can be higher, the economic return can be higher at low cost, and the invention has good performance in the aspects of industrial production and agricultural resource waste utilization and has wide application prospect.
Description
Technical Field
The invention belongs to the technical field of biology, relates to a preparation method of ethanol, and particularly relates to a preparation method of cellulosic ethanol.
Background
The growing agricultural technology generates more and more agricultural wastes, including crop straws, chaffs, fruit peels and the like, and how to treat the wastes is a serious problem. At present, the main utilization modes of agricultural wastes mainly focus on preparing feed and retting and returning to the field, and a large amount of agricultural wastes are not reasonably utilized, so that great resource waste is caused.
The research at present shows that the complex enzyme for hydrolyzing cellulose mainly comprises endoglucanase, exoglucanase and β -glucosidase, and the three enzymes are used for depolymerizing cellulose under the synergistic action.
Ethanol is one of the renewable energy sources with great potential to replace traditional fossil fuels, and is considered as one of the solutions to environmental problems caused by abuse of fossil fuels. It is reported that by 2024, the biofuel industry is expected to generate $ 9.5 billion, and by 2025, 30% of the petroleum fuels will be replaced; bioethanol is produced using plant biomass and can be obtained by direct fermentation of monosaccharides (called first-generation ethanol) or by saccharification of complex sugars and fermentation thereof (called second-generation ethanol). The main raw materials of the first generation bioethanol mainly comprise grains, including corn, wheat and the like, which contain starch to make crops, and the second generation bioethanol can be produced by using any cellulose material.
The cellulosic bioethanol is produced by using agricultural residues as raw materials to reduce raw material cost and increase value, but a large investment is required at the stage of transformation of first-generation bioethanol into second-generation bioethanol because of more steps and imperfect technology. However, the production raw materials are agricultural wastes, and do not interfere with grain production, so that the grain crisis is solved, the energy problem can be relieved, and meanwhile, the pollution of the agricultural wastes at the present stage is relieved. And with the continuous and deep research, the production cost is also continuously reduced. Corn stalks, banana stalks, bagasse, corncobs, cashew apple pomace, straws and the like are all raw materials for producing cellulose by bioethanol.
The traditional method for producing ethanol mainly takes grain crops as raw materials to produce and adopts a chemical synthesis method to prepare ethanol, the cost of the production raw materials for producing the ethanol by taking grains as the raw materials is higher, and the shortage of grain resources is caused; the chemical synthesis method needs large energy consumption, produces more byproducts in the production process, causes certain pollution to the environment, and is not suitable for later large-scale popularization and production. In order to solve the above problems, a method for producing ethanol, which reduces the cost of raw materials for production and by-products generated during the production process, is particularly necessary.
Disclosure of Invention
The invention aims to solve the defects of high cost of production raw materials, byproduct pollution in the production process and the like in the ethanol production in the current market, and provides a preparation method of cellulosic ethanol.
The technical scheme of the invention is as follows: the preparation method of the cellulosic ethanol is characterized by comprising the following steps:
(1) selecting strains which are screened in advance and degrade cellulose, namely brevibacterium frigostolerant, saccharomyces cerevisiae strains, namely BY4741 saccharomyces cerevisiae, inoculating the brevibacterium frigostolerant into a sterile L B culture medium for 8 hours after shaking at a speed of 220r/min, and inoculating the saccharomyces cerevisiae into a YPD culture medium for 8 hours after shaking at a speed of 220r/min, and taking the strain as a subsequent fermentation seed solution;
(2) preparing a fermentation enzyme-producing material: agricultural straw powder, sodium chloride, tryptone, yeast powder and distilled water;
(3) preparation of the required instruments and equipment: a full-temperature shaking flask cabinet, a headspace gas chromatograph, a centrifuge, a pH meter, a glass rod and a beaker;
(4) selecting crop straws as an experimental object, and performing fermentation culture on the agricultural wastes according to process parameters to prepare ethanol;
(5) the preparation method comprises the following specific steps:
(5-1) adding the L B fermentation seed liquid obtained in the step (1) into a sterile agricultural straw fermentation culture medium according to the inoculation amount of 4%;
(5-2) putting the inoculated fermentation enzyme-producing culture medium into a full-temperature shaking cabinet, and carrying out shaking culture at a constant temperature of 180r/min for 36 hours;
(5-3) adding YPD fermentation seed liquid into a fermentation bottle after shaking the bacteria for 36h according to the inoculation amount of 5%;
(5-4) placing the fermentation bottle after the secondary inoculation into a full-temperature shaking flask cabinet, and carrying out shaking culture at the constant temperature of 100r/min for 60 hours;
(5-5) inoculating YPD fermentation seed liquid, and storing 5m L fermentation liquid at 4 ℃ for later use every 12 h;
(5-6) centrifuging the obtained fermentation liquor at 10000r/min for 10min to obtain supernatant, namely the prepared material containing the cellulosic ethanol;
(5-7) determining the ethanol content in the cellulosic ethanol-containing material by headspace gas chromatography; separating to obtain the cellulosic ethanol.
The agricultural straw powder in the step (2) comprises at least one of corn straw powder, soybean straw powder, peanut straw powder, sorghum straw powder, wheat straw powder and rice straw powder.
The process parameters in the step (4) are that the concentration of the corn straw powder is 10%, the sodium chloride is 2%, the concentration of the tryptone is 3%, the yeast powder is 1.5%, the pH value of the culture medium is 6, the inoculation amount of the fermentation seed liquid L B is 2%, and the inoculation amount of the fermentation seed liquid YPD is 5%.
The pH value of the agricultural straw fermentation medium in the step (5-1) is 6.
The culture temperature in step (5-2) was 15 ℃.
The culture temperature in step (5-4) was 30 ℃.
In the material containing cellulosic ethanol in the step (5-7), the fermentation raw material is crop plant straw, so that the production cost is low; the cellulose degrading bacteria and the saccharomyces cerevisiae are synchronously fermented together, so that the production process is simple; no other pollution is generated in the production process, and meanwhile, agricultural wastes are utilized to reduce environmental pollution; the fermentation conditions are simple in the fermentation process, the reaction temperature is low, and the energy loss is reduced; the fermentation raw materials are agricultural wastes, and have wide sources and wide application range.
The invention has the beneficial effects that: according to the preparation method of the cellulose ethanol, the principle of the preparation method of the cellulose ethanol is clear, the effect of degrading cellulose to form the ethanol through co-fermentation of the brevibacterium frigostolerant strain and the saccharomyces cerevisiae is remarkable, the cellulose can be effectively decomposed, and the energy substance ethanol required by people is generated; the production raw materials used BY the invention are agricultural wastes, the strains are screened strains of brevibacterium frigosum (high growth and propagation speed, low growth temperature and good enzyme production performance) and the preserved strains of saccharomyces cerevisiae BY4741 (stable growth performance and high conversion rate), the preparation process of the cellulose ethanol is simple, the cost is low, no secondary pollution is caused to the environment, the crop straws can be degraded BY the cellulose decomposing bacteria, the required energy substances can be obtained, the demand on energy is low in the reaction process, less loss is caused to the energy, greater economic value can be generated, higher economic return can be obtained at lower cost, and the invention has better performance in the aspects of industrial production and utilization of agricultural resource wastes and has wide application prospect.
Drawings
FIG. 1 is a chromatogram of a standard of the present invention.
FIG. 2 is a standard curve of ethanol content versus peak area for the present invention.
FIG. 3 is a chromatogram for measuring the ethanol content in the fermentation liquid.
Detailed Description
The invention is further illustrated by the following examples in conjunction with the accompanying drawings:
as shown in figure 1, the preparation method of the cellulosic ethanol comprises the following steps of degrading cellulose by brevibacterium frigosum to obtain glucose, and converting the glucose into the cellulosic ethanol by using saccharomyces cerevisiae:
the method comprises the steps of selecting strains capable of degrading cellulose, namely brevibacterium frigostolerant strains and saccharomyces cerevisiae strains, namely BY4741 saccharomyces cerevisiae, and inoculating the brevibacterium frigostolerant strains into a sterile L B culture medium for shaking at a speed of 220r/min for 8 hours for later use, and inoculating the saccharomyces cerevisiae into a YPD culture medium for shaking at a speed of 220r/min for 8 hours for later use as a subsequent fermentation seed liquid.
Preparing a fermentation enzyme-producing material: agricultural straw powder, sodium chloride, tryptone, yeast powder and distilled water; preparation of the required instruments and equipment: a full-temperature shaking flask cabinet, a headspace gas chromatograph, a centrifuge, a pH meter, a glass rod and a fermentation flask.
The method comprises the following steps of selecting crop straws as an experimental object, carrying out fermentation culture on the agricultural waste to prepare ethanol according to process parameters, wherein the process parameters comprise that the concentration of corn straw powder is 10%, the concentration of sodium chloride is 2%, the concentration of tryptone is 3%, yeast powder is 1.5%, the pH value of a culture medium is 6, the inoculation amount of L B fermentation seed liquid is 2%, the inoculation amount of YPD fermentation seed liquid is 5%, the raw materials and the addition amount are shown in a table (1), and the preparation process is as follows:
adding the L B fermentation seed liquid obtained above into a sterile corn straw fermentation medium according to the inoculation amount of 2%, wherein the initial fermentation pH is 6, putting the inoculated fermentation enzyme production medium into a full-temperature shaking cabinet, carrying out shaking culture at the constant temperature of 180r/min at 15 ℃ for 36h, adding YPD fermentation seed liquid into a fermentation bottle after 36h of shaking culture according to the inoculation amount of 5%, putting the fermentation bottle after re-inoculation into the full-temperature shaking cabinet, carrying out shaking culture at the constant temperature of 100r/min at 30 ℃ for 60h, inoculating the YPD fermentation seed liquid, taking 5m L fermentation liquid every 12h, storing at 4 ℃ for later use, centrifuging the obtained fermentation liquid at 10000r/min for 10min to obtain a supernatant, namely the prepared material containing cellulosic ethanol, measuring the ethanol content in the material containing the cellulosic ethanol through headspace gas chromatography, and separating to obtain the cellulosic ethanol.
Watch (1)
Example 1
Determination of cellulose ethanol production yield
In order to explore the preparation yield of the cellulosic ethanol, the preparation fermentation is carried out under the conditions, and the ethanol content is measured by taking fresh fermentation liquor every 12 hours and carrying out three groups of parallel operations; centrifuging the fermentation liquor at 10000r/min for 10min to obtain supernatant, namely the prepared material containing the cellulosic ethanol; the ethanol content of the cellulosic ethanol-containing material was determined by headspace gas chromatography. And (5) preparing standard yeast by using absolute ethyl alcohol to perform gradient dilution. The chromatogram of the standard is shown in FIG. 1; the standard curve is shown in FIG. 2, the chromatogram for measuring the ethanol content in the fermentation broth is shown in FIG. 3, and the preparation amount of the cellulosic ethanol is shown in Table (2).
Watch (2)
From the graph (1), ethanol will peak before 3min, so that ethanol peak graph in subsequent determination samples can be determined, and standard curve of ethanol and peak area obtained by standard curve determination is shown in FIG. 2. The ethanol content in the materials is measured as shown in figure 3, the peak is before 3min, the result of the ethanol content can be obtained according to the measured peak diagram, and then the table (2) is obtained, the results shown in the table show that the cellulose ethanol is successfully produced by fermenting the cellulose decomposition bacteria and the saccharomyces cerevisiae, the ethanol yield is gradually increased along with the lapse of the fermentation time, and the reason that the early content is low is probably because the saccharomyces cerevisiae is just connected into a fermentation bottle and is in a self-replication proliferation stage, and then the ethanol conversion rate is higher. Therefore, the production method has better performance in the aspect of agricultural waste conversion and can be used for practical application.
The experiments show that the production cost is low by using crop plant straws as raw materials to produce the cellulosic ethanol; the cellulose degrading bacteria and the saccharomyces cerevisiae are synchronously fermented together, so that the production process is simple; no other pollution is generated in the production process, and meanwhile, agricultural wastes are utilized to reduce environmental pollution; the fermentation conditions are simple in the fermentation process, the reaction temperature is low, and the energy loss is reduced; the fermentation raw materials are agricultural wastes, and have wide sources and wide application range. The method has good development prospect in the aspects of agricultural waste treatment and industrial production.
Claims (7)
1. The preparation method of the cellulosic ethanol is characterized by comprising the following steps:
(1) selecting strains which are screened in advance and degrade cellulose, namely brevibacterium frigostolerant, saccharomyces cerevisiae strains, namely BY4741 saccharomyces cerevisiae, inoculating the brevibacterium frigostolerant into a sterile L B culture medium for 8 hours after shaking at a speed of 220r/min, and inoculating the saccharomyces cerevisiae into a YPD culture medium for 8 hours after shaking at a speed of 220r/min, and taking the strain as a subsequent fermentation seed solution;
(2) preparing a fermentation enzyme-producing material: agricultural straw powder, sodium chloride, tryptone, yeast powder and distilled water;
(3) preparation of the required instruments and equipment: a full-temperature shaking flask cabinet, a headspace gas chromatograph, a centrifuge, a pH meter, a glass rod and a beaker;
(4) selecting crop straws as an experimental object, and performing fermentation culture on the agricultural wastes according to process parameters to prepare ethanol;
(5) the preparation method comprises the following specific steps:
(5-1) adding the L B fermentation seed liquid obtained in the step (1) into a sterile agricultural straw fermentation culture medium according to the inoculation amount of 4%;
(5-2) putting the inoculated fermentation enzyme-producing culture medium into a full-temperature shaking cabinet, and carrying out shaking culture at a constant temperature of 180r/min for 36 hours;
(5-3) adding YPD fermentation seed liquid into a fermentation bottle after shaking the bacteria for 36h according to the inoculation amount of 5%;
(5-4) placing the fermentation bottle after the secondary inoculation into a full-temperature shaking flask cabinet, and carrying out shaking culture at the constant temperature of 100r/min for 60 hours;
(5-5) inoculating YPD fermentation seed liquid, and storing 5m L fermentation liquid at 4 ℃ for later use every 12 h;
(5-6) centrifuging the obtained fermentation liquor at 10000r/min for 10min to obtain supernatant, namely the prepared material containing the cellulosic ethanol;
(5-7) determining the ethanol content in the cellulosic ethanol-containing material by headspace gas chromatography; separating to obtain the cellulosic ethanol.
2. The method for preparing cellulosic ethanol according to claim 1, wherein the method comprises the following steps: the agricultural straw powder in the step (2) is at least one of corn straw powder, soybean straw powder, peanut straw powder, sorghum straw powder, wheat straw powder and rice straw powder.
3. The method for preparing cellulosic ethanol as claimed in claim 1, wherein the process parameters in step (4) are that the concentration of the corn straw powder is 10%, the concentration of sodium chloride is 2%, the concentration of tryptone is 3%, the yeast powder is 1.5%, the pH of the culture medium is =6, the inoculation amount of L B fermentation seed solution is 2%, and the inoculation amount of YPD fermentation seed solution is 5%.
4. The method for preparing cellulosic ethanol according to claim 1, wherein the method comprises the following steps: the pH value of the agricultural straw fermentation medium in the step (5-1) is 6.
5. The method for preparing cellulosic ethanol according to claim 1, wherein the method comprises the following steps: the culture temperature in step (5-2) was 15 ℃.
6. The method for preparing cellulosic ethanol according to claim 1, wherein the method comprises the following steps: the culture temperature in step (5-4) was 30 ℃.
7. The method for preparing cellulosic ethanol according to claim 1, wherein the method comprises the following steps: in the material containing cellulosic ethanol in the step (5-7), the fermentation raw material is crop plant straw, so that the production cost is low; the cellulose degrading bacteria and the saccharomyces cerevisiae are synchronously fermented together, so that the production process is simple; no other pollution is generated in the production process, and meanwhile, agricultural wastes are utilized to reduce environmental pollution; the fermentation conditions are simple in the fermentation process, the reaction temperature is low, and the energy loss is reduced; the fermentation raw materials are agricultural wastes, and have wide sources and wide application range.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111424024A (en) * | 2020-05-22 | 2020-07-17 | 扬州大学 | Preparation and use methods of low-temperature cellulase |
| CN112014495A (en) * | 2020-08-14 | 2020-12-01 | 北京首钢朗泽新能源科技有限公司 | Detection method and application of fusel oil component in ethanol fermentation liquor |
| CN112014495B (en) * | 2020-08-14 | 2022-11-22 | 北京首钢朗泽科技股份有限公司 | Detection method and application of fusel oil component in ethanol fermentation liquor |
| CN117025713A (en) * | 2023-09-21 | 2023-11-10 | 北京工商大学 | Method for preparing ethanol by composite bacterial system |
| CN117025713B (en) * | 2023-09-21 | 2024-02-13 | 北京工商大学 | Method for preparing ethanol by composite bacterial system |
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