CN107540454A - A kind of alga fertilizer preparation method using sea-tangle as raw material - Google Patents
A kind of alga fertilizer preparation method using sea-tangle as raw material Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
A kind of alga fertilizer preparation method using sea-tangle as raw material, sea-tangle combines the pueraria root residue process for fixation of preprocess method and enzyme by colloid mill and high pressure homogenizer, obtained marine alga is homogenized through immobilised enzymes two step method enzymolysis process Biocatalytic Conversion, realize that algal polysaccharides and protein are degraded into the target of alga oligosaccharides and polypeptide respectively, the final continuous production processes established the sea-tangle enzymolysis based on continuous flowing stirred tank and prepare alga fertilizer, the centrifuged supernatant of enzymolysis liquid and sea-tangle residue, supernatant is through falling film evaporation into concentrate, ultrafiltrate is liquid seaweed extract；Sea-tangle digests the common fermentation such as residue and cake of camellia oleifera seeds into solid organic fertilizer, reaches the purpose that the full cell of sea-tangle utilizes.The present invention improves the sea-tangle level of resources utilization, improves kelp processing technology and the alga fertilizer level of production, realizes that enzyme is repeated and is used continuously, reduces the consumption of enzyme, reduce alga fertilizer production cost, product quality is good, pure natural, pollution-free degradable.
Description
Technical field
The invention belongs to chemical technology field, it is related to the preparation method of marine alga fertilizer.
Background technology
Marine alga fertilizer is the bio-feritlizer be processed into through science using sea-plant marine alga as primary raw material, mainly into
Divide is that the natural bioactivity substance for being advantageous to growth and development of plants and marine alga extracted from marine alga absorbs simultaneously richness from ocean
The mineral nutrient element of collection in vivo, including algal polysaccharides, phenols poly-compounds, mannitol, glycine betaine, plant growth are adjusted
Save material (basic element of cell division, gibberellin, auxin and abscisic acid etc.) and the micro member such as nitrogen, phosphorus, potassium and iron, boron, molybdenum, iodine
Element.In addition, being increase fertilizer efficiency and the chelation of fertilizer, appropriate humic acid and appropriate trace element have also been dissolved in.
Compared with conventional fertilizers, alga fertilizer has following distinguishing feature.
First, alga fertilizer is more comprehensive than conventional fertilizers nutrition, balanced, obvious effect of increasing production is grown after crop applying, and seldom go out
Existing nutritional deficiency disease.Alga fertilizer is advantageous to growth and development of plants using natural seaweed as primary raw material, containing what is largely extracted from marine alga
Natural bioactivity substance and marine alga absorbed from ocean and be enriched with mineral nutrient element in vivo, including marine alga is more
Sugar, phenols poly-compounds, mannitol, glycine betaine, plant growth regulating substance（The basic element of cell division, gibberellin, auxin and de-
Fall acid etc.）With the trace element such as nitrogen, phosphorus, potassium and iron, boron, molybdenum, iodine.In addition, be increase fertilizer efficiency and fertilizer chelation, part
Product has also dissolved in appropriate humic acid and appropriate trace element, can meet the needs of plant growth each stage is to nutrition.2nd,
Contain a large amount of disease-resistant factors and special composition, fertile medicine economic benefits and social benefits in alga fertilizer.Degeneration-resistant disease resistance significantly increases after crop applying, blade face
Pesticidal effects can be improved by spraying.Algal polysaccharides and oligosaccharide, mannitol, phenols poly-compounds, the beet contained in alga fertilizer
The materials such as alkali, alginic acid and natural antibiotics, there is significant inhibitory anti-virus, anthelminthic effect, really play fertile medicine effect, greatly
The width enhancing cold-resistant of crop, drought resisting, disease-resistant, resistant to lodging, salinity and alkalinity resistance, to epidemic disease, virosis, anthrax, downy mildew, grey mold, white
The stronger resistance of the generation such as powder disease, droop.In addition, the alginic acid in alga fertilizer can reduce the surface tension of water, in plant
Surface forms thin film, increases contact area, makes water-soluble substances be easier to enter plant through cauline leaf superficial cell film
Cell, plant is set most effectively to absorb the nutritional ingredient in seaweed extracted liquor.If alga fertilizer and insecticide, bactericide are mixed
Use, there is synergistic effect, sprinkling expense can be reduced.3rd, substantial amounts of high active ingredient is contained in alga fertilizer, plant easily absorbs.
Growing way is vigorous after crop applying, can significantly improve yield and the quality of crop.Specific algal polysaccharides in marine alga, alginic acid,
The materials such as highly unsaturated fatty acid, there is very high bioactivity, the production of the nonspecific activity factor in plant can be stimulated
It is raw.Meanwhile also contain natural plants growth regulator in alga fertilizer, such as：Auxin, cytokinin-like substance and gibberellin
Deng each hormone ratio is close in its ratio and terrestrial plant, has the function that to adjust Endogenous hormone balance very well.Crop applying
Afterwards, yield and quality significantly improve, and the industrial crops quality such as tobacco, cotton, flowers can be significantly improved, especially to booth vegetable
Crop, the effect that increases both production and value are very notable.4th, alga fertilizer contains abundant organic matter and the sustained release factor, fertilizer efficiency length, can improve soil
Earth Tiny ecosystem, native promoting root growth living and anti-continuous cropping.Alga fertilizer can directly make soil or soil is increased organic matter, activation soil by plant
Various beneficial microbes in earth.These microorganisms can play a part of catalyst in plant, microbial metabolism circulation, make
The biopotency increase of soil.The metabolin of plant and edaphon can provide more nutrients for plant.Meanwhile marine alga is more
The sequestering system that sugar is formed can be such that nutrition slowly discharges, and extend fertilizer efficiency.In addition, the native compound such as sodium alginate that it contains
It is natural soil conditioner, the formation of soil granular structure can be promoted, improves soil internal void space, consolidate in coordination soil,
Liquid, gas proportions, recover due to soil over-burden and chemical contamination and lose lac balance, increase geobiont
Vigor, promote the release of available nutrient, be advantageous to root growth, improve the resistance of crop and anti-continuous cropping ability.5th, alga fertilizer
Natural safety nuisance free.Traditional chemical fertilizer fertilizer efficiency is single, seriously polluted, and long-term use can cause soil texture to be destroyed.
Alga fertilizer category natural seaweed extract, there is good compatibility with terrestrial plant, it is nontoxic to people, animal, it is environmentally safe,
With other any chemical fertilizer it is all incomparable the advantages of, be put into organic food special fertilizer material abroad.Alga fertilizer polymerize
Various active material in ocean, possess nutrition comprehensively, disease-resistant yield-increasing, it is living native, nuisanceless many advantages, such as, be really meet it is existing
For the good fertilizer in agricultural development direction.
China's alga fertilizer industry is started late, and phase late 1990s begins one's study, into 21 century after obtained it is fast
Speed development.In terms of the mode of production, mainly there are chemical method, physical method, 3 kinds of biological method.Currently, domestic most manufacturing enterprises
Main chemical leaching test production alga fertilizer simple using cheap, is that highly basic high temperature can destroy using the maximum inferior position of chemical method
The activity of marine alga endogenous substance；Physics extraction method is better than chemical leaching test, and it is thin that the technique cooled down using high pressure low temperature reaches marine alga
The broken purpose of cell wall, obtained macromolecular substances are difficult to directly be absorbed by crop, and fertilizer efficiency is not high；Most preferably biological method,
Extracted using enzyme hydrolysis, the activity of endogenous substance can be made to preserve to greatest extent.Due to the costliness of biology enzyme price
And the complexity of enzymolysis process, the country only have several families to take bioanalysis i.e. enzyme extraction.
Develop alga fertilizer, China has raw material advantage, and about 1,500,000 tons of the annual marine alga total output in China, 80% above is artificial
Cultivation, the marine alga quantity currently used for system fertilizer is seldom, no more than the 10% of marine alga total amount；And China is large agricultural country, fertilizer demand
Amount is very huge, therefore the development space of alga fertilizer industry is also very big.Sea-tangle is the quality raw materials for producing alga fertilizer.On the one hand it is
Extend sea-tangle industrial chain, improve sea-tangle utilization ratio；On the other hand alga fertilizer production cost is reduced；Sea-tangle living things catalysis is developed to turn
It is great to change alga fertilizer technical meaning.
The content of the invention
Remained high for alga fertilizer raw materials for production cost, marine alga is not implemented full cell and utilized in alga fertilizer production process
Problem.It is an object of the invention to provide a kind of alga fertilizer preparation method using sea-tangle as raw material, sea-tangle is through colloid mill and high pressure
After homogenizer combination pretreatment, by establishing immobilised enzymes two step method enzymolysis process, in continuously flowing stirred tank
Alga fertilizer continuous production and enzyme recycling are realized, improves production efficiency, reduces the cost of enzyme；Caused sea-tangle in enzymolysis process
The fermenting and producing solid-state seaweed organic fertilizer such as residue and cake of camellia oleifera seeds, this method realize that the full cell of sea-tangle utilizes, greatly reduces enzyme
Consume, be greatly reduced alga fertilizer production cost, produce alga fertilizer is pure natural, degradable, quality is good, environmental protection, reduce resource
Waste.
The purpose of the present invention is achieved through the following technical solutions.
A kind of alga fertilizer preparation method using sea-tangle as raw material of the present invention, according to the following steps.
(1) sea-tangle pre-processes：Sea-tangle is cleaned with running water；Soaked 12 hours, changed with running water again within during which every 2 hours
Water 1 time；Sea-tangle after immersion is shredded with knife, and broken sea-tangle crushes through colloid mill.Kelp paste and distilled water are with volume ratio 1 after crushing:3
~ 5 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：20 ~ 40 MPas of pressure, cycle-index 3 ~ 5 times, flow
1000 ls/h, room temperature condition.
(2) first step enzyme digestion reaction：Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:3 ~ 6 mix, the company of being placed in
Afterflow is moved in stirred tank, and cellulase is placed in together with agitating shaft through pueraria root residue immobilization, immobilized cellulase
The wire netting inframe of rotation, according to sea-tangle and cellulase mass ratio 5 ~ 50:1 addition immobilized cellulase, adjusts pH to 5.0
~ 6.0,50 ~ 100 revs/min of speed of agitator, after stirring 10 ~ 20 minutes, speed of agitator is adjusted to 30 ~ 100 revs/min, temperature control
System is at 40 ~ 60 DEG C；After immobilized cellulase Biocatalytic Conversion 1.5 ~ 2 hours, start continuous feed and discharging, reaction terminate
Stop charging and discharging within first 0.5 ~ 1.5 hour.
(3) second step enzyme digestion reaction：Discharging enters in another continuous flowing stirred tank in step (2), alkalescence
Protease is through pueraria root residue immobilization, and immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by alkalescence
The mg/litre of protease concentration 100 ~ 6000 adds immobilization alkali protease, pH to 5.5 ~ 7.5 is adjusted, by 80 ~ 160 revs/min
After stirring 6 ~ 18 minutes, speed of agitator is adjusted to 40 ~ 120 revs/min, and temperature control is at 55 ~ 65 DEG C；Reaction starting 1.5 ~ 2 is small
Shi Hou, starts continuous feed and discharging, and reaction terminates to stop charging and discharging in first 0.5 ~ 1.5 hour.
(4) by the enzymolysis liquid that step (3) obtains through centrifugation（4000 ~ 8000 revs/min, 4 ~ 10 minutes, room temperature condition）
Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, 10 ~ 50 ls/h of evaporation capacity, temperature 45 ~ 65
DEG C, 0.05 ~ 0.20 MPa of pressure, cocnentration factor 1.1 ~ 1.6, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, pressure
0.4 MPa, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.
(5) by the sea-tangle residue in step (4) and cake of camellia oleifera seeds, pericarp, waste dish, brewage waste residue, chicken manure with mass ratio 1:
2~4:4~6:5~7:2~3:3 ~ 6 heap fermentations, 10 ~ 20 cubic metres of volume, 40 ~ 60 centimetres of height, 45 ~ 60 DEG C of temperature, by turning over
Temperature, fermentation time 30 ~ 60 days are controlled to fermentation materials.Fermentation ends obtain high-quality solid organic fertilizer.
Continuous feed and discharge method are taken in step (2) of the present invention, speed is consistent, for 0.25 ~ 2 kilogram/it is small
When.
Pueraria root residue described in step (2) of the present invention and (3), preprocess method are as follows：Caused by kudzu-vine root powder production process
Pueraria root residue distilled water flushing 3 ~ 5 times, is placed in beaker, and pueraria root residue is with 5% ~ 20% Eusol with mass ratio 1:3 ~ 10 is mixed
It is even, vibrated in shaking table, after being vibrated 5 ~ 20 minutes by 50 ~ 100 revs/min, adjustment of rotational speed is 25 ~ 100 revs/min, temperature control
System is at 25 ~ 40 DEG C, duration of oscillation 12 ~ 36 hours, and vibration terminates rear pueraria root residue distilled water flushing 3 ~ 5 times.
Immobilized cellulase described in step (2) of the present invention, process for fixation are as follows：Cellulase is dissolved in 0.03
~ 0.25 mol/L phosphate buffer, pH of cushioning fluid are 5.0 ~ 6.5, and enzyme concentration is 10 ~ 100 g/l；Pueraria root residue after pretreatment
With cellulase solution with mass ratio 1:2 ~ 10 mix, and are vibrated in shaking table, after being vibrated 10 ~ 30 minutes by 50 ~ 100 revs/min,
Adjustment of rotational speed is 25 ~ 100 revs/min, and at 20 ~ 40 DEG C, duration of oscillation 18 ~ 36 hours, vibration secures temperature control after terminating
The pueraria root residue of cellulase distilled water flushing 3 ~ 5 times.
Continuous feed and discharge method are taken in step (3) of the present invention, speed is consistent, and is 0.25 ~ 2 kg/hr.
Immobilization alkali protease described in step (3) of the present invention, enzyme immobilizatio method are as follows：Alkali protease is molten
For solution in 0.03 ~ 0.25 mol/L phosphate buffer, pH of cushioning fluid is 5.0 ~ 6.5, and enzyme concentration is 5 ~ 100 g/l；Pretreatment
Pueraria root residue afterwards is with alkaline protease solution with mass ratio 1:2 ~ 10 mix, and are vibrated in shaking table, by 50 ~ 100 revs/min of vibrations
After 10 ~ 30 minutes, adjustment of rotational speed is 25 ~ 100 revs/min, and temperature control duration of oscillation 18 ~ 36 hours, vibrates at 30 ~ 50 DEG C
The pueraria root residue of alkali protease distilled water flushing 3 ~ 5 times is secured after end.
First step enzyme digestion reaction described in step (2) of the present invention, mainly by the algal polysaccharides in the structures such as sea-tangle cell membrane
The expression of the differential proteins such as crop can be induced by changing into the products such as the high oligosaccharides of the utilization ratios such as crop, alginic acid, and preventing and treating is made
The related disease such as thing.
Second step enzyme digestion reaction described in step (3) of the present invention, primarily to sea-tangle protein is catalytically converted into spy
The products such as specific polypeptide, amino acid.
Pericarp described in step (5) of the present invention is banana skin, watermelon peel or apple skin, waste dish be Chinese cabbage, romaine lettuce or
Lettuce waste dish, it is brewed spirit waste residue to brewage waste residue.
The present invention establishes a kind of alga fertilizer preparation method using sea-tangle as raw material, is turned based on the catalysis of immobilised enzymes two step method
Change sea-tangle, finally establish the continuous production processes that sea-tangle enzymolysis prepares alga fertilizer, enzymolysis liquid kills centrifuged supernatant after enzyme
With sea-tangle residue, supernatant through falling film evaporation into concentrate be liquid seaweed extract；Sea-tangle enzymolysis residue and cake of camellia oleifera seeds etc. are sent out altogether
Ferment reaches the purpose that the full cell of sea-tangle utilizes into solid organic fertilizer.The sea-tangle level of resources utilization is improved, protects environment, is realized
Enzyme repeats and continuous use, greatly reduces the consumption of enzyme, greatly reduces alga fertilizer production cost, practicality is very strong, is easy to advise
Modelling, it is good and be all-natural product to produce alga fertilizer quality, pollution-free degradable, is that one kind meets industrialization demand, environment
The new method of friendly.
Embodiment
The present invention will be described further by following examples.
Sea-tangle and cake of camellia oleifera seeds used in following examples are commercially available.
Pericarp and waste dish used in following examples are respectively collected from fruit wholesale market and the market of farm produce.
Chicken manure used in following examples is collected from chicken farm.
Cellulase and alkali protease used in following examples are purchased from Sigma-Aldrich companies.
Embodiment 1.
Sea-tangle is cleaned with running water, then soaked 12 hours with running water, changes water 1 time within during which every 2 hours；Sea after immersion
Band is shredded (wide with knife：0.5 cm, it is long：5 cms), broken sea-tangle crushes through colloid mill.Kelp paste and distillation after crushing
Water is with volume ratio 1:3 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：20 MPas of pressure, cycle-index 3
It is secondary, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：Use distilled water flushing
3 times, it is placed in beaker, pueraria root residue is with 5% Eusol with mass ratio 1:3 mix, and vibrate in shaking table, are shaken by 50 revs/min
After swinging 5 minutes, adjustment of rotational speed is 25 revs/min, and for temperature control at 25 DEG C, duration of oscillation 12 hours, vibration terminates rear pueraria root residue
With distilled water flushing 3 times, for immobilised enzymes.Cellulase immobilization method：Cellulase is dissolved in 0.03 mol/L phosphoric acid
Buffer solution, pH of cushioning fluid 5.0, enzyme concentration are 10 g/l；Pueraria root residue and cellulase solution are with mass ratio 1 after pretreatment:
2 mix, and are vibrated in shaking table, and after being vibrated 10 minutes by 50 revs/min, adjustment of rotational speed is 25 revs/min, and temperature control is 20
DEG C, duration of oscillation 18 hours, vibration secures the pueraria root residue of cellulase distilled water flushing 3 times after terminating.Alkali protease
Process for fixation it is as follows：Alkali protease is dissolved in 0.03 mol/L phosphate buffer, pH of cushioning fluid 5.0, enzyme concentration
For 5 g/l；Pretreated pueraria root residue is with alkaline protease solution with mass ratio 1:2 mix, vibrated in shaking table, by 50 turns/
After minute vibration 10 minutes, adjustment of rotational speed is 50 revs/min, and temperature control duration of oscillation 18 hours, is vibrated after terminating at 30 DEG C
Secure the pueraria root residue of alkali protease distilled water flushing 3 times.
Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:3 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to sea-tangle and enzyme mass ratio 5:1 addition
Immobilised enzymes, pH to 5.0 being adjusted, 50 revs/min of speed of agitator, after stirring 10 minutes, speed of agitator is adjusted to 30 revs/min,
Temperature control is at 40 DEG C；After immobilised enzymes Biocatalytic Conversion 1.5 hours, start continuous feed and discharging, input and output material speed are equal
For 0.25 kg/hr, reaction terminates to stop charging and discharging in first 0.5 hour.Discharging enters another 50 liters continuous flowing stirrings
In tank reactor, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by the milli of enzyme concentration 100
G/l addition immobilised enzymes, adjust pH to 5.5, by 80 revs/min stirring 6 minutes after, speed of agitator is adjusted to 40 revs/min,
Temperature control is at 55 DEG C；After reaction starting 1.5 hours, starting continuous feed and discharging, input and output material speed is 0.25 kilogram/and it is small
When, reaction terminates to stop charging and discharging in first 0.5 hour.The enzymolysis liquid of discharging is through centrifugation（4000 revs/min, 4 minutes, room temperature
Condition）Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, 10 ls/h of evaporation capacity, temperature 45
DEG C, 0.05 MPa of pressure, cocnentration factor 1.1, (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure are wrong through ultrafiltration for concentrate
The meter per second of flow velocity rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Sea-tangle residue and cake of camellia oleifera seeds, banana
Skin, Chinese cabbage waste dish, brewed spirit waste residue, chicken manure are with mass ratio 1:2:4:5:2:3 heap fermentations, 10 cubic metres of volume, height
40 centimetres, temperature 45 C, temperature, fermentation time 30 days are controlled by translating into fermentation materials.Fermentation ends obtain high-quality solid-state
Organic fertilizer.
Embodiment 2.
Sea-tangle is cleaned with running water, then soaked 12 hours with running water, changes water 1 time within during which every 2 hours；Sea after immersion
Band is shredded (wide with knife：0.5 cm, it is long：5 cms), broken sea-tangle crushes through colloid mill.Kelp paste and distillation after crushing
Water is with volume ratio 1:5 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：40 MPas of pressure, cycle-index 5
It is secondary, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：Use distilled water flushing
5 times, it is placed in beaker, pueraria root residue is with 20% Eusol with mass ratio 1:10 mix, and are vibrated in shaking table, by 150 revs/min
After clock vibrates 20 minutes, adjustment of rotational speed is 100 revs/min, and temperature control duration of oscillation 36 hours, is vibrated after terminating at 40 DEG C
Pueraria root residue distilled water flushing 5 times, for immobilised enzymes.Cellulase immobilization method：Cellulase be dissolved in 0.25 mole/
Phosphate buffer, pH of cushioning fluid 6.5 are risen, enzyme concentration is 100 g/l；After pretreatment pueraria root residue and cellulase solution with
Mass ratio 1:10 are mixed, and are vibrated in shaking table, and by 100 revs/min of vibrations, temperature control duration of oscillation 36 hours, is shaken at 40 DEG C
Swing and the pueraria root residue of cellulase distilled water flushing 5 times is secured after terminating.The process for fixation of alkali protease is as follows：Alkalescence
Protease is dissolved in 0.25 mol/L phosphate buffer, pH of cushioning fluid 6.5, and enzyme concentration is 100 g/l；It is pretreated
Pueraria root residue is with alkaline protease solution with mass ratio 1:10 mix, and are vibrated in shaking table, by 100 revs/min of vibrations, temperature control
At 50 DEG C, duration of oscillation 36 hours, vibration secures the pueraria root residue of alkali protease distilled water flushing 5 times after terminating.
Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:6 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to sea-tangle and enzyme mass ratio 50:1 adds
Being fixed enzyme, pH to 6.0 is adjusted, 100 revs/min of speed of agitator, temperature control is at 60 DEG C；Immobilised enzymes Biocatalytic Conversion
After 2 hours, it is 2 kgs/hr to start continuous feed and discharging, input and output material speed, and reaction terminates to stop charging in first 1.5 hours
And discharging.Discharging enters in another 50 liters continuous flowing stirred tanks, and immobilization alkali protease is placed in and agitating shaft
The wire netting inframe rotated together, immobilised enzymes is added by the mg/litre of enzyme concentration 6000, pH to 7.5 is adjusted, by 160 revs/min
After stirring 18 minutes, speed of agitator is adjusted to 120 revs/min, and temperature control is at 65 DEG C；After reaction starting 2 hours, start continuous
Charging and discharging, input and output material speed is 2 kgs/hr, and reaction terminates to stop charging and discharging in first 1.5 hours.The enzyme of discharging
Liquid is solved through centrifugation（8000 revs/min, 10 minutes, room temperature condition）Obtain supernatant and sea-tangle residue, supernatant falling film evaporation skill
Art is concentrated, and 50 ls/h of evaporation capacity, 65 DEG C of temperature, 0.20 MPa of pressure, cocnentration factor 1.6, concentrate is through ultrafiltration (fenestra
0.02 micron of footpath, temperature room temperature, 0.4 MPa of pressure, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain afterwards
Liquid seaweed extract.Sea-tangle residue is with cake of camellia oleifera seeds, apple skin, lettuce waste dish, brewed spirit waste residue, chicken manure with mass ratio 1:4:6:7:
3:6 heap fermentations, 20 cubic metres of volume, 60 centimetres of height, temperature 60 C, temperature is controlled by translating into fermentation materials, fermented
60 days time.Fermentation ends obtain high-quality solid organic fertilizer.
Embodiment 3.
Sea-tangle is cleaned with running water, then soaked 12 hours with running water, changes water 1 time within during which every 2 hours；Sea after immersion
Band is shredded (wide with knife：0.5 cm, it is long：5 cms), broken sea-tangle crushes through colloid mill.Kelp paste and distillation after crushing
Water is with volume ratio 1:4 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：30 MPas of pressure, cycle-index 4
It is secondary, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：Use distilled water flushing
4 times, it is placed in beaker, pueraria root residue is with 10% Eusol with mass ratio 1:6 mix, and are vibrated in shaking table, by 100 revs/min
After vibration 10 minutes, adjustment of rotational speed is 75 revs/min, and for temperature control at 30 DEG C, duration of oscillation 24 hours, vibration terminates the rear root of kudzu vine
Slag distilled water flushing 4 times, for immobilised enzymes.Cellulase immobilization method：Cellulase is dissolved in 0.10 mol/L phosphorus
Acid buffer, pH of cushioning fluid 6.0, enzyme concentration are 50 g/l；Pueraria root residue and cellulase solution are with mass ratio after pretreatment
1:6 are mixed, and are vibrated in shaking table, and by 75 revs/min of vibrations, temperature control duration of oscillation 24 hours, is vibrated after terminating at 30 DEG C
Secure the pueraria root residue of cellulase distilled water flushing 4 times.The process for fixation of alkali protease is as follows：Alkali protease is molten
Solution is 50 g/l in 0.10 mol/L phosphate buffer, pH of cushioning fluid 6.0, enzyme concentration；Pretreated pueraria root residue with
Alkaline protease solution is with mass ratio 1:6 mix, and are vibrated in shaking table, and after being vibrated 20 minutes by 75 revs/min, adjustment of rotational speed is
50 revs/min, temperature control at 40 DEG C, use by duration of oscillation 24 hours, vibration secures alkali protease pueraria root residue after terminating
Distilled water flushing 4 times.
Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:5 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to sea-tangle and enzyme mass ratio 25:1 adds
Being fixed enzyme, pH to 5.5 is adjusted, 75 revs/min of speed of agitator, after stirring 15 minutes, speed of agitator is adjusted to 60 revs/min
Clock, temperature control is at 50 DEG C；After immobilised enzymes Biocatalytic Conversion 2.0 hours, start continuous feed and discharging, input and output material speed
It it is 1 kg/hr, reaction terminates to stop charging and discharging in first 1 hour.Discharging enters another 50 liters continuous flowing agitator tank formulas
In reactor, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by 30000 milligrams of enzyme concentration/
Addition immobilised enzymes is risen, adjusts pH to 6.0, after being stirred 12 minutes by 120 revs/min, speed of agitator is adjusted to 80 revs/min,
Temperature control is at 60 DEG C；After reaction starting 2.0 hours, it is 1 kg/hr to start continuous feed and discharging, input and output material speed,
Reaction terminates to stop charging and discharging in first 1 hour.The enzymolysis liquid of discharging is through centrifugation（6000 revs/min, 8 minutes, room temperature condition）
Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, 30 ls/h of evaporation capacity, 55 DEG C of temperature, pressure
0.10 MPa of power, cocnentration factor 1.3, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure, cross-flow speed
The meter per second of rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Sea-tangle residue and cake of camellia oleifera seeds, watermelon peel, life
Dish waste dish, brewed spirit waste residue, chicken manure are with mass ratio 1:3:5:6:3:5 heap fermentations, 15 cubic metres of volume, 50 centimetres of height,
Temperature 50 C, temperature, fermentation time 45 days are controlled by translating into fermentation materials.Fermentation ends obtain high-quality solid organic fertilizer.
Comparative example 1.
Sea-tangle is cleaned with running water, then soaked 12 hours with running water, changes water 1 time within during which every 2 hours；Sea after immersion
Band is shredded (wide with knife：0.5 cm, it is long：5 cms).Broken sea-tangle is with distilled water with mass ratio 1:3 mix, and are placed in 50
Rise in continuous flowing stirred tank, do not add immobilised enzymes, adjust pH to 5.0,50 revs/min of speed of agitator, stirring
After 10 minutes, speed of agitator is adjusted to 30 revs/min, and temperature control is at 40 DEG C；After reaction starting 1.5 hours, start continuously to enter
Material and discharging, input and output material speed is 0.25 kg/hr, and reaction terminates to stop charging and discharging in first 0.5 hour.Discharging enters
In another 50 liters continuous flowing stirred tanks, enzyme is not added, adjusts pH to 5.5, after being stirred 6 minutes by 80 revs/min,
Speed of agitator is adjusted to 40 revs/min, and temperature control is at 55 DEG C；After reaction starting 1.5 hours, start continuous feed and discharging,
Input and output material speed is 0.25 kg/hr, and reaction terminates to stop charging and discharging in first 0.5 hour.Out-feed liquid is through centrifugation（4000
Rev/min, 4 minutes, room temperature condition）Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, evaporation capacity
10 ls/h, temperature 45 C, 0.05 MPa of pressure, cocnentration factor 1.1, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature chamber
Temperature, 0.4 MPa of pressure, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract, sea-tangle residue afterwards
It is directly discarded.
Comparative example 2.
Sea-tangle is cleaned with running water, then soaked 12 hours with running water, changes water 1 time within during which every 2 hours；Sea after immersion
Band is shredded (wide with knife：0.5 cm, it is long：5 cms), broken sea-tangle is with distilled water with mass ratio 1:6 mix, and are placed in 50
Rise in continuous flowing stirred tank, do not add enzyme, adjust pH to 6.0,100 revs/min of speed of agitator, stir 20 minutes
Afterwards, speed of agitator is adjusted to 100 revs/min, and temperature control is at 60 DEG C；After reaction starting 2 hours, start continuous feed and go out
Material, input and output material speed is 2 kgs/hr, and reaction terminates to stop charging and discharging in first 1.5 hours.Discharging enters another continuous
Flow in stirred tank, do not add enzyme, adjust pH to 7.5, after being stirred 18 minutes by 160 revs/min, speed of agitator is adjusted
Whole is 120 revs/min, and temperature control is at 65 DEG C；After reaction starting 2 hours, start continuous feed and discharging, input and output material speed are equal
For 2 kgs/hr, reaction terminates to stop charging and discharging in first 1.5 hours.Out-feed liquid is through centrifugation（8000 revs/min, 10 minutes,
Room temperature condition）Supernatant and sea-tangle residue are obtained, supernatant falling film evaporation technology is concentrated, 50 ls/h of evaporation capacity, temperature
65 DEG C of degree, 0.20 MPa of pressure, cocnentration factor 1.6, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, pressure 0.4 million
Pa, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Sea-tangle residue is directly discarded.
Comparative example 3.
Commercially available common foliar fertilizer.
Above example 1-3 and comparative example 1-3 application effect are as follows.
Crop field grape manure trial
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather temperature
With, sunshine is enriched, and makes a clear distinction between the four seasons, the summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-18.6
℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.80, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Kyoto grape.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 667 square metres.
Test and carried out in 2016, cell sets section ridging, vine 200 plants/acre of specification of plantation, 750 grams of Seedling Stage
Foliar fertilizer, 150 kilograms are watered, is stirred, foliage-spray is once；The Berry Growth phase, 1500 grams of foliar fertilizers, 150 kilograms are watered,
Foliage-spray stir once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate the disease incidences such as the big brown spot of grape, yellowtop, black rot, cracked fruit and weight method measure vintage,
Spectrophotometry grape soluble sugar content.
The alga fertilizer of table 1 handles grape effect.
Field rice manure trial.
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather
Gently, sunshine is enriched, and makes a clear distinction between the four seasons, and summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-
18.6℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.60, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Y two excellent 1.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 2000 square metres.
Test and carried out in 2016, cell sets section ridging, conventional to plant specification, 1500 grams of foliar fertilizers of Seedling Stage, converts
150 kilograms of water, stirs, and foliage-spray is once；Ear growth period, 3000 grams of foliar fertilizers, be watered 150 kilograms, stir
Foliage-spray is once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate rice blast, the sheath and culm blight of rice, the sheath and culm blight of rice, etc. disease incidence and weight method measure rice yield.
The alga fertilizer of table 2 handles rice effect.
Crop field tomato manure trial.
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather
Gently, sunshine is enriched, and makes a clear distinction between the four seasons, and summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-
18.6℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.80, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Rosy clouds powder tomato.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 667 square metres.
Test and carried out in 2016, cell sets section ridging, tomato 500 plants/acre of specification of plantation, 500 grams of Seedling Stage
Foliar fertilizer, 100 kilograms are watered, is stirred, foliage-spray is once；Bear fruit growth period, 1000 grams of foliar fertilizers, be watered 100 kilograms,
Foliage-spray stir once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate the disease incidences such as tomato droop, sclerotiniose, verticillium wilt and weight method measure tomato production.
The alga fertilizer of table 3 handles tomato effect.
Different fertilization is can be seen that to the disease such as grape fruit split incidence of disease, grape from table 1,2 and 3 result of the tests
The influence of yield and soluble sugar content reaches significant difference level, and soluble sugar content height is related to grape quality.It is solvable
Property sugared content is higher, and grape quality is better.Different fertilization is to the disease such as rice blast incidence of disease and tomato droop
Influence etc. the disease incidence of disease, rice and tomato production reaches significant difference level.Crop field grape, rice and tomato fertilizer efficiency
It is all highest that the alga fertilizer that test example 2 is prepared, which applies rear grape, rice and tomato production, grape, rice and west
The red persimmon incidence of disease is all minimum.It can be drawn from result of the test, alga fertilizer of the present invention can promote grape, rice and tomato life
It is long, effectively prevent and treat grape, rice and tomato disease.
Claims (7)
- A kind of 1. alga fertilizer preparation method using sea-tangle as raw material, it is characterized in that according to the following steps：(1) sea-tangle pre-processes：Sea-tangle is cleaned with running water；Soaked 12 hours with running water again, change water 1 within during which every 2 hours It is secondary；Sea-tangle after immersion is shredded with knife, and broken sea-tangle crushes through colloid mill；Kelp paste and distilled water are with volume ratio 1 after crushing:3~5 Mix, suspension is handled through high pressure homogenizer；Homogenizer condition of work：20 ~ 40 MPas of pressure, cycle-index 3 ~ 5 times, flow 1000 ls/h, room temperature condition；(2) first step enzyme digestion reaction：Sea-tangle homogenate and distilled water are with mass ratio 1 after pretreatment:3 ~ 6 mix, and are placed in continuous stream In dynamic stirred tank, through pueraria root residue immobilization, immobilized cellulase is placed in be rotated cellulase together with agitating shaft Wire netting inframe, according to sea-tangle and cellulase mass ratio 5 ~ 50:1 addition immobilized cellulase, regulation pH to 5.0 ~ 6.0,50 ~ 100 revs/min of speed of agitator, after stirring 10 ~ 20 minutes, speed of agitator is adjusted to 30 ~ 100 revs/min, temperature control System is at 40 ~ 60 DEG C；After immobilized cellulase Biocatalytic Conversion 1.5 ~ 2 hours, start continuous feed and discharging, reaction terminate Stop charging and discharging within first 0.5 ~ 1.5 hour；Second step enzyme digestion reaction：Discharging enters in another continuous flowing stirred tank in step (2), alkali protease warp Pueraria root residue immobilization, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, dense by alkali protease Spend 100 ~ 60000 mg/litres addition immobilization alkali protease, adjust pH to 5.5 ~ 7.5, by 80 ~ 160 revs/min stirring 6 ~ After 18 minutes, speed of agitator is adjusted to 40 ~ 120 revs/min, and temperature control is at 55 ~ 65 DEG C；After reaction starting 1.5 ~ 2 hours, open Beginning continuous feed and discharging, reaction terminate to stop charging and discharging in first 0.5 ~ 1.5 hour；(4) under the enzymolysis liquid room temperature condition that step (3) obtains, 4000 ~ 8000 revs/min, centrifuge 4 ~ 10 minutes, obtain supernatant Liquid and sea-tangle residue, supernatant falling film evaporation technology are concentrated, 10 ~ 50 ls/h of evaporation capacity, 45 ~ 65 DEG C of temperature, pressure 0.05 ~ 0.20 MPa, cocnentration factor 1.1 ~ 1.6, concentrate obtains liquid seaweed extract after ultrafiltration；(5) the sea-tangle residue in step (4) and cake of camellia oleifera seeds, pericarp, waste dish, brewage waste residue, chicken manure with mass ratio 1:2~4:4~ 6:5~7:2~3:3 ~ 6 heap fermentations, 10 ~ 20 cubic metres of volume, 40 ~ 60 centimetres of height, 45 ~ 60 DEG C of temperature, by translating into fermentation Material controls temperature, fermentation time 30 ~ 60 days；Fermentation ends obtain high-quality solid organic fertilizer.
- A kind of 2. alga fertilizer preparation method using sea-tangle as raw material according to claim 1, it is characterized in that being adopted in step (2) Continuous feed and discharge method are taken, speed is consistent, and is 0.25 ~ 2 kg/hr.
- A kind of 3. alga fertilizer preparation method using sea-tangle as raw material according to claim 1, it is characterized in that institute in step (2) Stating pueraria root residue preprocess method is：The distilled water flushing 3 ~ 5 times of pueraria root residue caused by kudzu-vine root powder production process, is placed in beaker, Pueraria root residue is with 5% ~ 20% Eusol with mass ratio 1:3 ~ 10 mix, vibrated in shaking table, by 50 ~ 150 revs/min vibration 5 ~ After 20 minutes, adjustment of rotational speed is 25 ~ 100 revs/min, and temperature control is at 25 ~ 40 DEG C, and duration of oscillation 12 ~ 36 hours, vibration terminates Pueraria root residue distilled water flushing 3 ~ 5 times afterwards.
- A kind of 4. alga fertilizer preparation method using sea-tangle as raw material according to claim 1, it is characterized in that institute in step (2) Stating cellulase immobilization method is：Cellulase is dissolved in 0.03 ~ 0.25 mol/L phosphate buffer, and pH of cushioning fluid is 5.0 ~ 6.5, enzyme concentration is 10 ~ 100 g/l；Pueraria root residue and cellulase solution are with mass ratio 1 after pretreatment:2 ~ 10 mix, Vibrated in shaking table, after being vibrated 10 ~ 30 minutes by 50 ~ 100 revs/min, adjustment of rotational speed is 25 ~ 100 revs/min, and temperature control exists 20 ~ 40 DEG C, duration of oscillation 18 ~ 36 hours, vibration secures the pueraria root residue of cellulase distilled water flushing 3 ~ 5 times after terminating.
- A kind of 5. alga fertilizer preparation method using sea-tangle as raw material according to claim 1, it is characterized in that being adopted in step (3) Continuous feed and discharge method are taken, speed is consistent, and is 0.25 ~ 2 kg/hr.
- A kind of 6. alga fertilizer preparation method using sea-tangle as raw material according to claim 1, it is characterized in that institute in step (3) The basic protein enzyme immobilization method stated is as follows：Alkali protease is dissolved in 0.03 ~ 0.25 mol/L phosphate buffer, buffering Liquid pH value is 5.0 ~ 6.5, and enzyme concentration is 5 ~ 100 g/l；Pueraria root residue and alkaline protease solution are with mass ratio 1 after pretreatment:2~ 10 mix, and are vibrated in shaking table, and after being vibrated 10 ~ 30 minutes by 50 ~ 100 revs/min, adjustment of rotational speed is 25 ~ 100 revs/min, temperature At 30 ~ 50 DEG C, duration of oscillation 18 ~ 36 hours, vibration secures the pueraria root residue distilled water of alkali protease after terminating for degree control Rinse 3 ~ 5 times.
- A kind of 7. alga fertilizer preparation method using sea-tangle as raw material according to claim 1, it is characterized in that institute in step (5) The pericarp stated is banana skin, watermelon peel or apple skin, and waste dish is Chinese cabbage, romaine lettuce or lettuce waste dish, brewages waste residue and is made for white wine Make waste residue.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108585979A (en) * | 2018-05-11 | 2018-09-28 | 天津市辰圣海洋生物技术有限公司 | It is a kind of using kelp as the alga fertilizer preparation method of raw material |
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