CN107540453A - A kind of alga fertilizer preparation method using Enteromorpha as raw material - Google Patents
A kind of alga fertilizer preparation method using Enteromorpha as raw material Download PDFInfo
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- CN107540453A CN107540453A CN201710927207.7A CN201710927207A CN107540453A CN 107540453 A CN107540453 A CN 107540453A CN 201710927207 A CN201710927207 A CN 201710927207A CN 107540453 A CN107540453 A CN 107540453A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
A kind of alga fertilizer preparation method using Enteromorpha as raw material, Enteromorpha combines the pueraria root residue process for fixation of preprocess method and enzyme by colloid mill and high pressure homogenizer, obtained marine alga is homogenized through immobilised enzymes two step method enzymolysis process Biocatalytic Conversion, realize that algal polysaccharides and protein are degraded into the target of alga oligosaccharides and polypeptide respectively, the final continuous production processes established the Enteromorpha enzymolysis based on continuous flowing stirred tank and prepare alga fertilizer, the centrifuged supernatant of enzymolysis liquid and Enteromorpha residue, supernatant is through falling film evaporation into concentrate, ultrafiltrate is liquid seaweed extract；Enteromorpha digests the common fermentation such as residue and cake of camellia oleifera seeds into solid organic fertilizer, reaches the purpose that the full cell of Enteromorpha utilizes.The present invention improves the Enteromorpha level of resources utilization, improves Enteromorpha process technology and the alga fertilizer level of production, realizes that enzyme is repeated and is used continuously, reduces the consumption of enzyme, reduce alga fertilizer production cost, product quality is good, pure natural, pollution-free degradable.
Description
Technical field
The invention belongs to chemical technology field, it is related to the preparation method of marine alga fertilizer.
Background technology
Marine alga fertilizer is the bio-feritlizer be processed into through science using sea-plant marine alga as primary raw material, mainly into
Divide is that the natural bioactivity substance for being advantageous to growth and development of plants and marine alga extracted from marine alga absorbs simultaneously richness from ocean
The mineral nutrient element of collection in vivo, including algal polysaccharides, phenols poly-compounds, mannitol, glycine betaine, plant growth are adjusted
Save material (basic element of cell division, gibberellin, auxin and abscisic acid etc.) and the micro member such as nitrogen, phosphorus, potassium and iron, boron, molybdenum, iodine
Element.In addition, being increase fertilizer efficiency and the chelation of fertilizer, appropriate humic acid and appropriate trace element have also been dissolved in.
Compared with conventional fertilizers, alga fertilizer has following distinguishing feature.
First, alga fertilizer is more comprehensive than conventional fertilizers nutrition, balanced, obvious effect of increasing production is grown after crop applying, and seldom go out
Existing nutritional deficiency disease.Alga fertilizer is advantageous to growth and development of plants using natural seaweed as primary raw material, containing what is largely extracted from marine alga
Natural bioactivity substance and marine alga absorbed from ocean and be enriched with mineral nutrient element in vivo, including marine alga is more
Sugar, phenols poly-compounds, mannitol, glycine betaine, plant growth regulating substance（The basic element of cell division, gibberellin, auxin and de-
Fall acid etc.）With the trace element such as nitrogen, phosphorus, potassium and iron, boron, molybdenum, iodine.In addition, be increase fertilizer efficiency and fertilizer chelation, part
Product has also dissolved in appropriate humic acid and appropriate trace element, can meet the needs of plant growth each stage is to nutrition.2nd,
Contain a large amount of disease-resistant factors and special composition, fertile medicine economic benefits and social benefits in alga fertilizer.Degeneration-resistant disease resistance significantly increases after crop applying, blade face
Pesticidal effects can be improved by spraying.Algal polysaccharides and oligosaccharide, mannitol, phenols poly-compounds, the beet contained in alga fertilizer
The materials such as alkali, alginic acid and natural antibiotics, there is significant inhibitory anti-virus, anthelminthic effect, really play fertile medicine effect, greatly
The width enhancing cold-resistant of crop, drought resisting, disease-resistant, resistant to lodging, salinity and alkalinity resistance, to epidemic disease, virosis, anthrax, downy mildew, grey mold, white
The stronger resistance of the generation such as powder disease, droop.In addition, the alginic acid in alga fertilizer can reduce the surface tension of water, in plant
Surface forms thin film, increases contact area, makes water-soluble substances be easier to enter plant through cauline leaf superficial cell film
Cell, plant is set most effectively to absorb the nutritional ingredient in seaweed extracted liquor.If alga fertilizer and insecticide, bactericide are mixed
Use, there is synergistic effect, sprinkling expense can be reduced.3rd, substantial amounts of high active ingredient is contained in alga fertilizer, plant easily absorbs.
Growing way is vigorous after crop applying, can significantly improve yield and the quality of crop.Specific algal polysaccharides in marine alga, alginic acid,
The materials such as highly unsaturated fatty acid, there is very high bioactivity, the production of the nonspecific activity factor in plant can be stimulated
It is raw.Meanwhile also contain natural plants growth regulator in alga fertilizer, such as：Auxin, cytokinin-like substance and gibberellin
Deng each hormone ratio is close in its ratio and terrestrial plant, has the function that to adjust Endogenous hormone balance very well.Crop applying
Afterwards, yield and quality significantly improve, and the industrial crops quality such as tobacco, cotton, flowers can be significantly improved, especially to booth vegetable
Crop, the effect that increases both production and value are very notable.4th, alga fertilizer contains abundant organic matter and the sustained release factor, fertilizer efficiency length, can improve soil
Earth Tiny ecosystem, native promoting root growth living and anti-continuous cropping.Alga fertilizer can directly make soil or soil is increased organic matter, activation soil by plant
Various beneficial microbes in earth.These microorganisms can play a part of catalyst in plant, microbial metabolism circulation, make
The biopotency increase of soil.The metabolin of plant and edaphon can provide more nutrients for plant.Meanwhile marine alga is more
The sequestering system that sugar is formed can be such that nutrition slowly discharges, and extend fertilizer efficiency.In addition, the native compound such as sodium alginate that it contains
It is natural soil conditioner, the formation of soil granular structure can be promoted, improves soil internal void space, consolidate in coordination soil,
Liquid, gas proportions, recover due to soil over-burden and chemical contamination and lose lac balance, increase geobiont
Vigor, promote the release of available nutrient, be advantageous to root growth, improve the resistance of crop and anti-continuous cropping ability.5th, alga fertilizer
Natural safety nuisance free.Traditional chemical fertilizer fertilizer efficiency is single, seriously polluted, and long-term use can cause soil texture to be destroyed.
Alga fertilizer category natural seaweed extract, there is good compatibility with terrestrial plant, it is nontoxic to people, animal, it is environmentally safe,
With other any chemical fertilizer it is all incomparable the advantages of, be put into organic food special fertilizer material abroad.Alga fertilizer polymerize
Various active material in ocean, possess nutrition comprehensively, disease-resistant yield-increasing, it is living native, nuisanceless many advantages, such as, be really meet it is existing
For the good fertilizer in agricultural development direction.
China's alga fertilizer industry is started late, and phase late 1990s begins one's study, into 21 century after obtained it is fast
Speed development.In terms of the mode of production, mainly there are chemical method, physical method, 3 kinds of biological method.Currently, domestic most manufacturing enterprises
Main chemical leaching test production alga fertilizer simple using cheap, is that highly basic high temperature can destroy using the maximum inferior position of chemical method
The activity of marine alga endogenous substance；Physics extraction method is better than chemical leaching test, and it is thin that the technique cooled down using high pressure low temperature reaches marine alga
The broken purpose of cell wall, obtained macromolecular substances are difficult to directly be absorbed by crop, and fertilizer efficiency is not high；Most preferably biological method,
Extracted using enzyme hydrolysis, the activity of endogenous substance can be made to preserve to greatest extent.Due to the costliness of biology enzyme price
And the complexity of enzymolysis process, the country only have several families to take bioanalysis i.e. enzyme extraction.
Develop alga fertilizer, China has raw material advantage, and about 1,500,000 tons of the annual marine alga total output in China, 80% above is artificial
Cultivation, the marine alga quantity currently used for system fertilizer is seldom, no more than the 10% of marine alga total amount；And China is large agricultural country, fertilizer demand
Amount is very huge, therefore the development space of alga fertilizer industry is also very big.Enteromorpha is the quality raw materials for producing alga fertilizer.On the one hand it is
Extend Enteromorpha industrial chain, improve Enteromorpha utilization ratio；On the other hand alga fertilizer production cost is reduced；Enteromorpha living things catalysis is developed to turn
It is great to change alga fertilizer technical meaning.
The content of the invention
Remained high for alga fertilizer raw materials for production cost, Enteromorpha is not implemented full cell and utilized in alga fertilizer production process
Problem.It is an object of the invention to provide a kind of alga fertilizer preparation method using Enteromorpha as raw material, Enteromorpha is through colloid mill and high pressure
After homogenizer combination pretreatment, by establishing immobilised enzymes two step method enzymolysis process, in continuously flowing stirred tank
Alga fertilizer continuous production and enzyme recycling are realized, improves production efficiency, reduces the cost of enzyme；Caused Enteromorpha in enzymolysis process
The fermenting and producing solid-state seaweed organic fertilizer such as residue and cake of camellia oleifera seeds, this method realize that the full cell of Enteromorpha utilizes, greatly reduces enzyme
Consume, be greatly reduced alga fertilizer production cost, produce alga fertilizer is pure natural, degradable, quality is good, environmental protection, reduce resource
Waste.
The purpose of the present invention is achieved through the following technical solutions.
A kind of alga fertilizer preparation method using Enteromorpha as raw material of the present invention, according to the following steps：
(1) Enteromorpha pre-processes：Enteromorpha is cleaned with running water；Soaked 36 hours with running water again, change water 1 within during which every 4 hours
It is secondary；Enteromorpha after immersion is shredded with knife, and broken Enteromorpha crushes through colloid mill；Enteromorpha mud and distilled water are with volume ratio 1 after crushing:10~
30 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：50 ~ 70 MPas of pressure, cycle-index 3 ~ 5 times, flow
1000 ls/h, room temperature condition.
(2) first step enzyme digestion reaction：Enteromorpha homogenate and distilled water are with mass ratio 1 after pretreatment:15 ~ 20 mix, the company of being placed in
Afterflow is moved in stirred tank, and cellulase is placed in together with agitating shaft through pueraria root residue immobilization, immobilized cellulase
The wire netting inframe of rotation, according to Enteromorpha and cellulase mass ratio 40 ~ 100:1 addition immobilized cellulase, regulation pH to
5.0 ~ 6.0,150 ~ 250 revs/min of speed of agitator, after stirring 5 ~ 15 minutes, speed of agitator is adjusted to 40 ~ 100 revs/min, temperature
Degree control is at 40 ~ 55 DEG C；After immobilized cellulase Biocatalytic Conversion 1 ~ 3 hour, start continuous feed and discharging, reaction knot
Stop charging and discharging within 1 ~ 2 hour before beam.
(3) second step enzyme digestion reaction：Discharging enters in another continuous flowing stirred tank in step (2), alkalescence
Protease is through pueraria root residue immobilization, and immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by alkalescence
The mg/litre of protease concentration 500 ~ 12000 adds immobilization alkali protease, pH to 6.0 ~ 7.0 is adjusted, by 150 ~ 300 revs/min
After clock stirs 6 ~ 15 minutes, speed of agitator is adjusted to 40 ~ 100 revs/min, and temperature control is at 45 ~ 70 DEG C；Reaction starting 1 ~ 3 is small
Shi Hou, starts continuous feed and discharging, and reaction terminates to stop charging and discharging in first 1 ~ 2 hour.
(4) under the enzymolysis liquid room temperature condition for obtaining step (3), 5000 ~ 10000 revs/min, centrifuge 4 ~ 8 minutes, obtain
To supernatant and Enteromorpha residue, supernatant falling film evaporation technology is concentrated, 10 ~ 60 ls/h of evaporation capacity, temperature 60 ~ 75
DEG C, 0.06 ~ 0.24 MPa of pressure, cocnentration factor 1.1 ~ 2.0, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, pressure
0.4 MPa, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.
(5) by the Enteromorpha residue in step (4) and cake of camellia oleifera seeds, pericarp, waste dish, brewage waste residue, chicken manure with mass ratio 1:
1~2:2~6:3~5:1~4:2 ~ 5 heap fermentations, 7 ~ 13 cubic metres of volume, 60 ~ 85 centimetres of height, 55 ~ 75 DEG C of temperature, by translating into
Fermentation materials control temperature, fermentation time 15 ~ 30 days；Fermentation ends obtain high-quality solid organic fertilizer.
Continuous feed and discharge method are taken in step (2) of the present invention, speed is consistent, and is 1 ~ 5 kg/hr.
Pueraria root residue described in step (2) of the present invention and (3), preprocess method are as follows：Caused by kudzu-vine root powder production process
Pueraria root residue distilled water flushing 3 ~ 5 times, is placed in beaker, and pueraria root residue is with 20% ~ 50% Eusol with mass ratio 1:10 ~ 20 is mixed
It is even, vibrated in shaking table, after being vibrated 5 ~ 20 minutes by 100 ~ 200 revs/min, adjustment of rotational speed is 50 ~ 150 revs/min, temperature control
System is at 25 ~ 40 DEG C, duration of oscillation 12 ~ 18 hours, and vibration terminates rear pueraria root residue distilled water flushing 3 ~ 5 times.
Immobilized cellulase described in step (2) of the present invention, process for fixation are as follows：Cellulase is dissolved in 0.01
~ 0.05 mol/L phosphate buffer, pH of cushioning fluid are 5.0 ~ 7.5, and enzyme concentration is 1 ~ 30 g/l；After pretreatment pueraria root residue with
Cellulase solution is with mass ratio 1:20 ~ 50 mix, and are vibrated in shaking table, after being vibrated 4 ~ 16 minutes by 120 ~ 240 revs/min,
Adjustment of rotational speed is 60 ~ 150 revs/min, and at 20 ~ 35 DEG C, duration of oscillation 16 ~ 32 hours, vibration secures temperature control after terminating
The pueraria root residue of cellulase distilled water flushing 3 ~ 5 times
Continuous feed and discharge method are taken in step (3) of the present invention, speed is consistent, and is 1 ~ 5 kg/hr.
Immobilization alkali protease described in step (3) of the present invention, enzyme immobilizatio method are as follows：Alkali protease is molten
For solution in 0.01 ~ 0.05 mol/L phosphate buffer, pH of cushioning fluid is 6.5 ~ 7.5, and enzyme concentration is 10 ~ 100 g/l；Pretreatment
Pueraria root residue and alkaline protease solution are with mass ratio 1 afterwards:15 ~ 25 mix, and are vibrated in shaking table, by 120 ~ 240 revs/min of vibrations
After 4 ~ 12 minutes, adjustment of rotational speed is 60 ~ 150 revs/min, and temperature control at 20 ~ 35 DEG C, tie by duration of oscillation 16 ~ 32 hours, vibration
The pueraria root residue of alkali protease distilled water flushing 3 ~ 5 times is secured after beam.
First step enzyme digestion reaction described in step (2) of the present invention, mainly by the algal polysaccharides in the structures such as Enteromorpha cell membrane
The expression of the differential proteins such as crop can be induced by changing into the products such as the high oligosaccharides of the utilization ratios such as crop, alginic acid, and preventing and treating is made
The related disease such as thing.
Second step enzyme digestion reaction described in step (3) of the present invention, primarily to enteromorpha protein matter is catalytically converted into spy
The products such as specific polypeptide, amino acid.
Pericarp described in step (5) of the present invention is banana skin, watermelon peel or apple skin, waste dish be Chinese cabbage, romaine lettuce or
Lettuce waste dish, it is brewed spirit waste residue to brewage waste residue.
The present invention establishes a kind of alga fertilizer preparation method using Enteromorpha as raw material, is turned based on the catalysis of immobilised enzymes two step method
Change Enteromorpha, finally establish the continuous production processes that Enteromorpha enzymolysis prepares alga fertilizer, enzymolysis liquid kills centrifuged supernatant after enzyme
With Enteromorpha residue, supernatant through falling film evaporation into concentrate be liquid seaweed extract；Enteromorpha enzymolysis residue and cake of camellia oleifera seeds etc. are sent out altogether
Ferment reaches the purpose that the full cell of Enteromorpha utilizes into solid organic fertilizer.The Enteromorpha level of resources utilization is improved, protects environment, is realized
Enzyme repeats and continuous use, greatly reduces the consumption of enzyme, greatly reduces alga fertilizer production cost, practicality is very strong, is easy to advise
Modelling, it is good and be all-natural product to produce alga fertilizer quality, pollution-free degradable, is that one kind meets industrialization demand, environment
The new method of friendly.
Embodiment
The present invention will be described further by following examples.
Enteromorpha and cake of camellia oleifera seeds used in following examples are commercially available.
Pericarp and waste dish used in following examples are respectively collected from fruit wholesale market and the market of farm produce.
Chicken manure used in following examples is collected from chicken farm.
Cellulase and alkali protease used in following examples are purchased from Sigma-Aldrich companies.
Embodiment 1.
Enteromorpha is cleaned with running water, then soaked 36 hours with running water, changes water 1 time within during which every 4 hours；Waterside after immersion
Tongue is shredded (wide with knife：0.5 cm, it is long：5 cms), broken Enteromorpha crushes through colloid mill.Enteromorpha mud and distillation after crushing
Water is with volume ratio 1:10 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：50 MPas of pressure, cycle-index 3
It is secondary, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：Use distilled water flushing
3 times, it is placed in beaker, pueraria root residue is with 20% Eusol with mass ratio 1:10 mix, and are vibrated in shaking table, by 100 revs/min
After clock vibrates 5 minutes, adjustment of rotational speed is 50 revs/min, and for temperature control at 25 DEG C, duration of oscillation 12 hours, vibration terminates rear Pueraria lobota
Root slag distilled water flushing 3 times, for immobilised enzymes.Cellulase immobilization method：Cellulase is dissolved in 0.01 mol/L
Phosphate buffer, pH of cushioning fluid 5.0, enzyme concentration are 1 g/l；Pueraria root residue and cellulase solution are with quality after pretreatment
Than 1:20 mix, and are vibrated in shaking table, and after being vibrated 4 minutes by 120 revs/min, adjustment of rotational speed is 60 revs/min, temperature control
At 20 DEG C, duration of oscillation 16 hours, vibration secures the pueraria root residue of cellulase distilled water flushing 3 times after terminating.Alkaline egg
White enzyme immobilizatio method is as follows：Alkali protease is dissolved in 0.01 mol/L phosphate buffer, pH of cushioning fluid 6.5, enzyme
Concentration is 10 g/l；Pretreated pueraria root residue is with alkaline protease solution with mass ratio 1:15 mix, and are vibrated in shaking table,
After being vibrated 4 minutes by 120 revs/min, adjustment of rotational speed is 60 revs/min, and temperature control duration of oscillation 16 hours, is shaken at 20 DEG C
Swing and the pueraria root residue of alkali protease distilled water flushing 3 times is secured after terminating.
Enteromorpha homogenate and distilled water are with mass ratio 1 after pretreatment:15 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to Enteromorpha and enzyme mass ratio 40:1 adds
Being fixed enzyme, pH to 5.0 is adjusted, 150 revs/min of speed of agitator, after stirring 5 minutes, speed of agitator is adjusted to 40 revs/min
Clock, temperature control is at 40 DEG C；After immobilised enzymes Biocatalytic Conversion 1 hour, start continuous feed and discharging, input and output material speed are equal
For 1 kg/hr, reaction terminates to stop charging and discharging in first 1 hour.It is anti-that discharging enters another 50 liters continuous flowing agitator tank formulas
Answer in device, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, adds by the mg/litre of enzyme concentration 500
Being fixed enzyme, pH to 6.0 is adjusted, after being stirred 6 minutes by 150 revs/min, speed of agitator is adjusted to 40 revs/min, temperature control
System is at 45 DEG C；After reaction starting 1 hour, it is 1 kg/hr to start continuous feed and discharging, input and output material speed, and reaction terminates
Stop charging and discharging within first 1 hour.The enzymolysis liquid of discharging is through centrifugation（5000 revs/min, 4 minutes, room temperature condition）Obtain supernatant
Liquid and Enteromorpha residue, supernatant falling film evaporation technology are concentrated, 10 ls/h of evaporation capacity, temperature 60 C, pressure 0.06 million
Pa, cocnentration factor 1.1, concentrate through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure, 3.6 meters of cross-flow rate/
Second, 800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Enteromorpha residue and cake of camellia oleifera seeds, banana skin, Chinese cabbage waste dish,
Brewed spirit waste residue, chicken manure are with mass ratio 1:1:2:3:1:2 heap fermentations, 7 cubic metres of volume, 60 centimetres of height, 55 DEG C of temperature,
Temperature, fermentation time 15 days are controlled by translating into fermentation materials.Fermentation ends obtain high-quality solid organic fertilizer.
Embodiment 2.
Enteromorpha is cleaned with running water, then soaked 36 hours with running water, changes water 1 time within during which every 4 hours；Waterside after immersion
Tongue is shredded (wide with knife：0.5 cm, it is long：5 cms), broken Enteromorpha crushes through colloid mill.Enteromorpha mud and distillation after crushing
Water is with volume ratio 1:30 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：70 MPas of pressure, cycle-index 5
It is secondary, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：Use distilled water flushing
5 times, it is placed in beaker, pueraria root residue is with 50% Eusol with mass ratio 1:20 mix, and are vibrated in shaking table, by 200 revs/min
After clock vibrates 20 minutes, adjustment of rotational speed is 150 revs/min, and temperature control duration of oscillation 18 hours, is vibrated after terminating at 40 DEG C
Pueraria root residue distilled water flushing 5 times, for immobilised enzymes.Cellulase immobilization method：Cellulase be dissolved in 0.05 mole/
Phosphate buffer, pH of cushioning fluid 7.5 are risen, enzyme concentration is 30 g/l；Pueraria root residue and cellulase solution are with matter after pretreatment
Measure ratio 1:50 mix, and are vibrated in shaking table, and after being vibrated 16 minutes by 240 revs/min, adjustment of rotational speed is 150 revs/min, temperature
Control is at 35 DEG C, duration of oscillation 32 hours, and vibration secures the pueraria root residue of cellulase distilled water flushing 5 times after terminating.Alkali
The process for fixation of property protease is as follows：Alkali protease is dissolved in 0.05 mol/L phosphate buffer, and pH of cushioning fluid is
7.5, enzyme concentration is 100 g/l；Pretreated pueraria root residue is with alkaline protease solution with mass ratio 1:25 mix, in shaking table
Middle vibration, after 240 revs/min of vibrations 12 minutes, adjustment of rotational speed is 150 revs/min, and temperature control is at 35 DEG C, duration of oscillation
32 hours, vibration secured the pueraria root residue of alkali protease distilled water flushing 5 times after terminating.
Enteromorpha homogenate and distilled water are with mass ratio 1 after pretreatment:20 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to Enteromorpha and enzyme mass ratio 100:1 adds
Being fixed enzyme, pH to 6.0 is adjusted, 250 revs/min of speed of agitator, after stirring 15 minutes, speed of agitator is adjusted to 100 revs/min
Clock, temperature control is at 55 DEG C；After immobilised enzymes Biocatalytic Conversion 3 hours, start continuous feed and discharging, input and output material speed are equal
For 5 kgs/hr, reaction terminates to stop charging and discharging in first 2 hours.It is anti-that discharging enters another 50 liters continuous flowing agitator tank formulas
Answer in device, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by the mg/litre of enzyme concentration 12000
Immobilised enzymes is added, adjusts pH to 7.0, after being stirred 15 minutes by 300 revs/min, speed of agitator is adjusted to 100 revs/min, temperature
Degree control is at 70 DEG C；After reaction starting 3 hours, it is 5 kgs/hr to start continuous feed and discharging, input and output material speed, reaction
Stop charging and discharging within 2 hours before end.The enzymolysis liquid of discharging is through centrifugation（10000 revs/min, 8 minutes, room temperature condition）Obtain
Supernatant and Enteromorpha residue, supernatant falling film evaporation technology are concentrated, 60 ls/h of evaporation capacity, 75 DEG C of temperature, pressure
0.24 MPa, cocnentration factor 2.0, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure, cross-flow rate
3.6 meter per seconds, 800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Enteromorpha residue and cake of camellia oleifera seeds, apple skin, lettuce
Waste dish, brewed spirit waste residue, chicken manure are with mass ratio 1:2:6:5:4:5 heap fermentations, 13 cubic metres of volume, 85 centimetres of height, temperature
75 DEG C of degree, temperature, fermentation time 30 days are controlled by translating into fermentation materials.Fermentation ends obtain high-quality solid organic fertilizer.
Embodiment 3.
Enteromorpha is cleaned with running water, then soaked 36 hours with running water, changes water 1 time within during which every 4 hours；Waterside after immersion
Tongue is shredded (wide with knife：0.5 cm, it is long：5 cms), broken Enteromorpha crushes through colloid mill.Enteromorpha mud and distillation after crushing
Water is with volume ratio 1:20 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：60 MPas of pressure, cycle-index 4
It is secondary, 1000 ls/h of flow, room temperature condition.Pueraria root residue preprocess method caused by kudzu-vine root powder production process：Use distilled water flushing
4 times, it is placed in beaker, pueraria root residue is with 35% Eusol with mass ratio 1:15 mix, and are vibrated in shaking table, by 150 revs/min
After clock vibrates 12 minutes, adjustment of rotational speed is 100 revs/min, and temperature control duration of oscillation 16 hours, is vibrated after terminating at 35 DEG C
Pueraria root residue distilled water flushing 4 times, for immobilised enzymes.Cellulase immobilization method：Cellulase be dissolved in 0.03 mole/
Phosphate buffer, pH of cushioning fluid 6.0 are risen, enzyme concentration is 15 g/l；Pueraria root residue and cellulase solution are with matter after pretreatment
Measure ratio 1:35 mix, and are vibrated in shaking table, and after being vibrated 10 minutes by 180 revs/min, adjustment of rotational speed is 105 revs/min, temperature
Control is at 30 DEG C, duration of oscillation 24 hours, and vibration secures the pueraria root residue of cellulase distilled water flushing 4 times after terminating.Alkali
The process for fixation of property protease is as follows：Alkali protease is dissolved in 0.03 mol/L phosphate buffer, and pH of cushioning fluid is
7.0, enzyme concentration is 55 g/l；Pretreated pueraria root residue is with alkaline protease solution with mass ratio 1:20 mix, in shaking table
Vibration, after being vibrated 8 minutes by 180 revs/min, adjustment of rotational speed is 105 revs/min, and for temperature control at 30 DEG C, duration of oscillation 24 is small
When, vibration secures the pueraria root residue of alkali protease distilled water flushing 4 times after terminating.
Enteromorpha homogenate and distilled water are with mass ratio 1 after pretreatment:18 mix, and are placed in 50 liters of continuous flowing agitator tank formula reactions
In device, immobilized cellulase is placed in the wire netting inframe rotated together with agitating shaft, according to Enteromorpha and enzyme mass ratio 70:1 adds
Being fixed enzyme, pH to 5.5 is adjusted, 200 revs/min of speed of agitator, after stirring 10 minutes, speed of agitator is adjusted to 70 revs/min
Clock, temperature control is at 45 DEG C；After immobilised enzymes Biocatalytic Conversion 2 hours, start continuous feed and discharging, input and output material speed are equal
For 3 kgs/hr, reaction terminates to stop charging and discharging in first 1.5 hours.Discharging enters another 50 liters continuous flowing agitator tank formulas
In reactor, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, by the mg/litre of enzyme concentration 6000
Immobilised enzymes is added, adjusts pH to 6.5, after being stirred 10 minutes by 250 revs/min, speed of agitator is adjusted to 70 revs/min, temperature
Degree control is at 55 DEG C；After reaction starting 2 hours, it is 3 kgs/hr to start continuous feed and discharging, input and output material speed, reaction
Stop charging and discharging within 1.5 hours before end.The enzymolysis liquid of discharging is through centrifugation（8000 revs/min, 6 minutes, room temperature condition）
To supernatant and Enteromorpha residue, supernatant falling film evaporation technology is concentrated, 35 ls/h of evaporation capacity, 65 DEG C of temperature, pressure
0.18 MPa, cocnentration factor 1.5, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure, cross-flow rate
3.6 meter per seconds, 800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Enteromorpha residue and cake of camellia oleifera seeds, watermelon peel, romaine lettuce
Waste dish, brewed spirit waste residue, chicken manure are with mass ratio 1:2:4:2:2:3 heap fermentations, 10 cubic metres of volume, 75 centimetres of height, temperature
60 DEG C of degree, temperature, fermentation time 20 days are controlled by translating into fermentation materials.Fermentation ends obtain high-quality solid organic fertilizer.
Comparative example 1.
Enteromorpha is cleaned with running water, then soaked 36 hours with running water, changes water 1 time within during which every 4 hours；Waterside after immersion
Tongue is shredded (wide with knife：0.5 cm, it is long：5 cms).Broken Enteromorpha is with distilled water with mass ratio 1:15 mix, and are placed in 50
Rise in continuous flowing stirred tank, do not add immobilised enzymes, adjust pH to 5.0,150 revs/min of speed of agitator, stirring
After 5 minutes, speed of agitator is adjusted to 40 revs/min, and temperature control is at 40 DEG C；Reaction starting 1 hour after, start continuous feed and
Discharging, input and output material speed is 1 kg/hr, and reaction terminates to stop charging and discharging in first 1 hour.Discharging enters another 50 liters
In continuous flowing stirred tank, enzyme is not added, adjusts pH to 6.0, after being stirred 6 minutes by 150 revs/min, stirring turns
Speed is adjusted to 40 revs/min, and temperature control is at 45 DEG C；After reaction starting 1 hour, start continuous feed and discharging, input and output material speed
Rate is 1 kg/hr, and reaction terminates to stop charging and discharging in first 1 hour.Out-feed liquid is through centrifugation（5000 revs/min, 4 points
Clock, room temperature condition）Supernatant and Enteromorpha residue are obtained, supernatant falling film evaporation technology is concentrated, 10 ls/h of evaporation capacity,
Temperature 60 C, 0.06 MPa of pressure, cocnentration factor 1.1, concentrate is through ultrafiltration (0.02 micron of membrane aperture, temperature room temperature, pressure 0.4
MPa, the meter per second of cross-flow rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards, Enteromorpha residue is directly discarded.
Comparative example 2.
Enteromorpha is cleaned with running water, then soaked 36 hours with running water, changes water 1 time within during which every 4 hours；Waterside after immersion
Tongue is shredded (wide with knife：0.5 cm, it is long：5 cms), broken Enteromorpha is with distilled water with mass ratio 1:20 mix, and are placed in 50
Rise in continuous flowing stirred tank, do not add enzyme, adjust pH to 6.0,250 revs/min of speed of agitator, stir 15 minutes
Afterwards, speed of agitator is adjusted to 100 revs/min, and temperature control is at 55 DEG C；After reaction starting 3 hours, start continuous feed and go out
Material, input and output material speed is 5 kgs/hr, and reaction terminates to stop charging and discharging in first 2 hours.Discharging enters another continuous stream
In dynamic stirred tank, enzyme is not added, adjusts pH to 7.0, after being stirred 15 minutes by 300 revs/min, speed of agitator adjustment
For 100 revs/min, temperature control is at 70 DEG C；After reaction starting 3 hours, start continuous feed and discharging, input and output material speed are
5 kgs/hr, reaction terminates to stop charging and discharging in first 2 hours.Out-feed liquid is through centrifugation（10000 revs/min, 8 minutes, room temperature
Condition）Supernatant and Enteromorpha residue are obtained, supernatant falling film evaporation technology is concentrated, 60 ls/h of evaporation capacity, temperature 75
DEG C, 0.24 MPa of pressure, cocnentration factor 2.0, (0.02 micron of membrane aperture, temperature room temperature, 0.4 MPa of pressure are wrong through ultrafiltration for concentrate
The meter per second of flow velocity rate 3.6,800 liters/square m/h) it is to obtain liquid seaweed extract afterwards.Enteromorpha residue is directly discarded.
Comparative example 3.
Commercially available common foliar fertilizer.
Above example 1-3 and comparative example 1-3 application effect are as follows.
Crop field grape manure trial.
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather
Gently, sunshine is enriched, and makes a clear distinction between the four seasons, and summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-
18.6℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.80, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Kyoto grape.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 667 square metres.
Test and carried out in 2016, cell sets section ridging, vine 200 plants/acre of specification of plantation, 750 grams of Seedling Stage
Foliar fertilizer, 150 kilograms are watered, is stirred, foliage-spray is once；The Berry Growth phase, 1500 grams of foliar fertilizers, 150 kilograms are watered,
Foliage-spray stir once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate the disease incidences such as the big brown spot of grape, yellowtop, black rot, cracked fruit and weight method measure vintage,
Spectrophotometry grape soluble sugar content.
The alga fertilizer of table 1 handles grape effect.
| Processing | Foliar fertilizer | Fertilization mode | The incidences of disease such as cracked fruit（%） | Vintage（Thousand grams/acre） | Soluble sugar content（Gram/100 grams） |
| 1 | Embodiment 1 | Each 1 time of seedling, Berry Growth | 5d | 2780a | 16.10c |
| 2 | Embodiment 2 | Each 1 time of seedling, Berry Growth | 6d | 2870a | 17.22b |
| 3 | Embodiment 3 | Each 1 time of seedling, Berry Growth | 6d | 2880a | 18.86a |
| 4 | Comparative example 1 | Each 1 time of seedling, Berry Growth | 33b | 1940c | 13.51e |
| 5 | Comparative example 2 | Each 1 time of seedling, Berry Growth | 40a | 1960c | 13.33e |
| 6 | Comparative example 3 | Each 1 time of seedling, Berry Growth | 27c | 2430b | 14.45d |
Field rice manure trial.
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather
Gently, sunshine is enriched, and makes a clear distinction between the four seasons, and summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-
18.6℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.60, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Y two excellent 1.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 2000 square metres.
Test and carried out in 2016, cell sets section ridging, conventional to plant specification, 1500 grams of foliar fertilizers of Seedling Stage, converts
150 kilograms of water, stirs, and foliage-spray is once；Ear growth period, 3000 grams of foliar fertilizers, be watered 150 kilograms, stir
Foliage-spray is once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate rice blast, the sheath and culm blight of rice, the sheath and culm blight of rice, etc. disease incidence and weight method measure rice yield.
The alga fertilizer of table 2 handles rice effect.
| Processing | Foliar fertilizer | Fertilization mode | The incidences of disease such as rice blast（%） | Rice yield（Thousand grams/acre） |
| 1 | Embodiment 1 | Each 1 time of seedling, heading growth | 7c | 750a |
| 2 | Embodiment 2 | Each 1 time of seedling, heading growth | 7c | 760b |
| 3 | Embodiment 3 | Each 1 time of seedling, heading growth | 6c | 870a |
| 4 | Comparative example 1 | Each 1 time of seedling, heading growth | 20b | 440d |
| 5 | Comparative example 2 | Each 1 time of seedling, heading growth | 20b | 600c |
| 6 | Comparative example 3 | Each 1 time of seedling, heading growth | 28a | 660c |
Crop field tomato manure trial.
Test site：The horizontal Jiang Zhen in Jiangxi Province Ji'an City Jian County.Belong to middle subtropical zone hills mountain city monsoon moist climate, weather
Gently, sunshine is enriched, and makes a clear distinction between the four seasons, and summer in winter length, spring and autumn is short, and abundant rainfall, frost-free period is longer, average temperature of the whole year 17.5-
18.6℃。
For trying soil：Red soil, nutrient content are：The g kg of organic matter 9.80, the full mg/kg of nitrogen 186.25 are quick-acting
The mg/kg of phosphorus 5.89, the mg/kg of available potassium 45.66, soil pH 5.8.
Experimental cultivar：Rosy clouds powder tomato.
Experimental design：This experiment sets 6 processing altogether, and specific processing is shown in Table 1, and often processing is repeated 3 times, random district's groups arrangement,
Experimental plot area is 667 square metres.
Test and carried out in 2016, cell sets section ridging, tomato 500 plants/acre of specification of plantation, 500 grams of Seedling Stage
Foliar fertilizer, 100 kilograms are watered, is stirred, foliage-spray is once；Bear fruit growth period, 1000 grams of foliar fertilizers, be watered 100 kilograms,
Foliage-spray stir once.Base manure and each processing field management method are identical, not spraying pesticide.
Investigate the disease incidences such as tomato droop, sclerotiniose, verticillium wilt and weight method measure tomato production.
The alga fertilizer of table 3 handles tomato effect.
| Processing | Foliar fertilizer | Fertilization mode | The incidences of disease such as droop（%） | Tomato production（Thousand grams/acre） |
| 1 | Embodiment 1 | Each 1 time of seedling, Berry Growth | 5c | 4350a |
| 2 | Embodiment 2 | Each 1 time of seedling, Berry Growth | 5c | 4220a |
| 3 | Embodiment 3 | Each 1 time of seedling, Berry Growth | 4c | 4360a |
| 4 | Comparative example 1 | Each 1 time of seedling, Berry Growth | 27b | 3360c |
| 5 | Comparative example 2 | Each 1 time of seedling, Berry Growth | 26b | 3340c |
| 6 | Comparative example 3 | Each 1 time of seedling, Berry Growth | 38a | 3590b |
Different fertilization is can be seen that to the disease such as grape fruit split incidence of disease, grape from table 1,2 and 3 result of the tests
The influence of yield and soluble sugar content reaches significant difference level, and soluble sugar content height is related to grape quality.It is solvable
Property sugared content is higher, and grape quality is better.Different fertilization is to the disease such as rice blast incidence of disease and tomato droop
Influence etc. the disease incidence of disease, rice and tomato production reaches significant difference level.Crop field grape, rice and tomato fertilizer efficiency
It is all highest that the alga fertilizer that test example 2 is prepared, which applies rear grape, rice and tomato production, grape, rice and west
The red persimmon incidence of disease is all minimum.It can be drawn from result of the test, alga fertilizer of the present invention can promote grape, rice and tomato life
It is long, effectively prevent and treat grape, rice and tomato disease.
Claims (7)
- A kind of 1. alga fertilizer preparation method using Enteromorpha as raw material, it is characterized in that according to the following steps：(1) Enteromorpha pre-processes：Enteromorpha is cleaned with running water；Soaked 36 hours with running water again, change water 1 within during which every 4 hours It is secondary；Enteromorpha after immersion is shredded with knife, and broken Enteromorpha crushes through colloid mill；Enteromorpha mud and distilled water are with volume ratio 1 after crushing:10~ 30 are mixed, and suspension is handled through high pressure homogenizer；Homogenizer condition of work：50 ~ 70 MPas of pressure, cycle-index 3 ~ 5 times, flow 1000 ls/h, room temperature condition；(2) first step enzyme digestion reaction：Enteromorpha homogenate and distilled water are with mass ratio 1 after pretreatment:15 ~ 20 mix, and are placed in continuous Flow in stirred tank, through pueraria root residue immobilization, immobilized cellulase is placed in be turned cellulase together with agitating shaft Dynamic wire netting inframe, according to Enteromorpha and cellulase mass ratio 40 ~ 100:1 addition immobilized cellulase, adjusts pH to 5.0 ~ 6.0,150 ~ 250 revs/min of speed of agitator, after stirring 5 ~ 15 minutes, speed of agitator is adjusted to 40 ~ 100 revs/min, temperature control System is at 40 ~ 55 DEG C；After immobilized cellulase Biocatalytic Conversion 1 ~ 3 hour, start continuous feed and discharging, before reaction terminates Stop charging and discharging within 1 ~ 2 hour；Second step enzyme digestion reaction：Discharging enters in another continuous flowing stirred tank in step (2), alkali protease warp Pueraria root residue immobilization, immobilization alkali protease is placed in the wire netting inframe rotated together with agitating shaft, dense by alkali protease Spend 500 ~ 12000 mg/litres addition immobilization alkali protease, adjust pH to 6.0 ~ 7.0, by 150 ~ 300 revs/min stirring 6 ~ After 15 minutes, speed of agitator is adjusted to 40 ~ 100 revs/min, and temperature control is at 45 ~ 70 DEG C；After reaction starting 1 ~ 3 hour, start Continuous feed and discharging, reaction terminate to stop charging and discharging in first 1 ~ 2 hour；(4) under the enzymolysis liquid room temperature condition that step (3) obtains, 5000 ~ 10000 revs/min, centrifuge 4 ~ 8 minutes, obtain supernatant Liquid and Enteromorpha residue, supernatant falling film evaporation technology are concentrated, 10 ~ 60 ls/h of evaporation capacity, 60 ~ 75 DEG C of temperature, pressure 0.06 ~ 0.24 MPa, cocnentration factor 1.1 ~ 2.0, concentrate obtains liquid seaweed extract after ultrafiltration；(5) the Enteromorpha residue in step (4) and cake of camellia oleifera seeds, pericarp, waste dish, brewage waste residue, chicken manure with mass ratio 1:1~2:2~ 6:3~5:1~4:2 ~ 5 heap fermentations, 7 ~ 13 cubic metres of volume, 60 ~ 85 centimetres of height, 55 ~ 75 DEG C of temperature, by translating into fermentate Expect to control temperature, fermentation time 15 ~ 30 days；Fermentation ends obtain high-quality solid organic fertilizer.
- A kind of 2. alga fertilizer preparation method using Enteromorpha as raw material according to claim 1, it is characterized in that being adopted in step (2) Continuous feed and discharge method are taken, speed is consistent, and is 1 ~ 5 kg/hr.
- A kind of 3. alga fertilizer preparation method using Enteromorpha as raw material according to claim 1, it is characterized in that institute in step (2) Stating pueraria root residue preprocess method is：The distilled water flushing 3 ~ 5 times of pueraria root residue caused by kudzu-vine root powder production process, is placed in beaker, Pueraria root residue is with 20% ~ 50% Eusol with mass ratio 1:10 ~ 20 mix, and vibrate in shaking table, are shaken by 100 ~ 200 revs/min After swinging 5 ~ 20 minutes, adjustment of rotational speed is 50 ~ 150 revs/min, and temperature control duration of oscillation 12 ~ 18 hours, vibrates at 25 ~ 40 DEG C Pueraria root residue distilled water flushing 3 ~ 5 times after end.
- A kind of 4. alga fertilizer preparation method using Enteromorpha as raw material according to claim 1, it is characterized in that institute in step (2) Stating cellulase immobilization method is：Cellulase is dissolved in 0.01 ~ 0.05 mol/L phosphate buffer, and pH of cushioning fluid is 5.0 ~ 7.5, enzyme concentration is 1 ~ 30 g/l；Pueraria root residue and cellulase solution are with mass ratio 1 after pretreatment:20 ~ 50 mix, Vibrated in shaking table, after being vibrated 4 ~ 16 minutes by 120 ~ 240 revs/min, adjustment of rotational speed is 60 ~ 150 revs/min, and temperature control exists 20 ~ 35 DEG C, duration of oscillation 16 ~ 32 hours, vibration secures the pueraria root residue of cellulase distilled water flushing 3 ~ 5 times after terminating.
- A kind of 5. alga fertilizer preparation method using Enteromorpha as raw material according to claim 1, it is characterized in that being adopted in step (3) Continuous feed and discharge method are taken, speed is consistent, and is 1 ~ 5 kg/hr.
- A kind of 6. alga fertilizer preparation method using Enteromorpha as raw material according to claim 1, it is characterized in that institute in step (3) The basic protein enzyme immobilization method stated is as follows：Alkali protease is dissolved in 0.01 ~ 0.05 mol/L phosphate buffer, buffering Liquid pH value is 6.5 ~ 7.5, and enzyme concentration is 10 ~ 100 g/l；Pueraria root residue and alkaline protease solution are with mass ratio 1 after pretreatment: 15 ~ 25 mix, and are vibrated in shaking table, and after being vibrated 4 ~ 12 minutes by 120 ~ 240 revs/min, adjustment of rotational speed is 60 ~ 150 revs/min Clock, temperature control at 20 ~ 35 DEG C, steam by duration of oscillation 16 ~ 32 hours, vibration secures alkali protease pueraria root residue after terminating Distilled water is rinsed 3 ~ 5 times.
- A kind of 7. alga fertilizer preparation method using Enteromorpha as raw material according to claim 1, it is characterized in that institute in step (5) The pericarp stated is banana skin, watermelon peel or apple skin, and waste dish is Chinese cabbage, romaine lettuce or lettuce waste dish, brewages waste residue and is made for white wine Make waste residue.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108409372A (en) * | 2018-05-11 | 2018-08-17 | 天津市辰圣海洋生物技术有限公司 | It is a kind of using Enteromorpha as the alga fertilizer preparation method of raw material |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101439997A (en) * | 2008-12-16 | 2009-05-27 | 青岛明月海藻集团有限公司 | Preparation of seaweed organic fertilizer |
| CN102391030A (en) * | 2011-08-15 | 2012-03-28 | 上海理工大学 | Organic seaweed fertilizer and application thereof as efficient plant growth regulator |
| CN102408282A (en) * | 2011-08-15 | 2012-04-11 | 上海理工大学 | Organic seaweed fertilizer and application thereof used as efficient saline soil conditioner |
| CN103204713A (en) * | 2013-01-09 | 2013-07-17 | 中国海洋大学生物工程开发有限公司 | Method for promoting high efficiency decomposition of enteromorpha powder by using enzymatically hydrolyzed sea-tangle residues and application of product |
| CN106915987A (en) * | 2017-03-28 | 2017-07-04 | 南昌大学 | A kind of preparation method of the special alga fertilizer of grape |
| CN107056346A (en) * | 2017-03-31 | 2017-08-18 | 南昌大学 | A kind of pure natural concentrated type epipremnum aureum nutrition liquid and preparation method thereof |
-
2017
- 2017-10-09 CN CN201710927207.7A patent/CN107540453A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101439997A (en) * | 2008-12-16 | 2009-05-27 | 青岛明月海藻集团有限公司 | Preparation of seaweed organic fertilizer |
| CN102391030A (en) * | 2011-08-15 | 2012-03-28 | 上海理工大学 | Organic seaweed fertilizer and application thereof as efficient plant growth regulator |
| CN102408282A (en) * | 2011-08-15 | 2012-04-11 | 上海理工大学 | Organic seaweed fertilizer and application thereof used as efficient saline soil conditioner |
| CN103204713A (en) * | 2013-01-09 | 2013-07-17 | 中国海洋大学生物工程开发有限公司 | Method for promoting high efficiency decomposition of enteromorpha powder by using enzymatically hydrolyzed sea-tangle residues and application of product |
| CN106915987A (en) * | 2017-03-28 | 2017-07-04 | 南昌大学 | A kind of preparation method of the special alga fertilizer of grape |
| CN107056346A (en) * | 2017-03-31 | 2017-08-18 | 南昌大学 | A kind of pure natural concentrated type epipremnum aureum nutrition liquid and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 刘英汉等: "《葛的栽培与葛根的加工利用》", 30 September 2002, 金盾出版社 * |
| 李斌等: "《食品酶学与酶工程》", 30 September 2017, 中国农业大学出版社 * |
| 陈宁等: "《高等学校专业教材 酶工程》", 30 June 2011, 中国轻工业出版社 * |
| 黄纯等: "《生物化学》", 30 June 2008, 中国医药科技出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108409372A (en) * | 2018-05-11 | 2018-08-17 | 天津市辰圣海洋生物技术有限公司 | It is a kind of using Enteromorpha as the alga fertilizer preparation method of raw material |
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