CN108409372A - It is a kind of using Enteromorpha as the alga fertilizer preparation method of raw material - Google Patents
It is a kind of using Enteromorpha as the alga fertilizer preparation method of raw material Download PDFInfo
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- CN108409372A CN108409372A CN201810448210.5A CN201810448210A CN108409372A CN 108409372 A CN108409372 A CN 108409372A CN 201810448210 A CN201810448210 A CN 201810448210A CN 108409372 A CN108409372 A CN 108409372A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, includes the following steps：S1, Enteromorpha is cleaned into air-dried crushing, obtains seaweed particle；S2, seaweed particle use is dissolved into seaweed colloidal solution；S3, it digests seaweed colloidal solution to obtain mixed liquor；S4, mixed liquor is centrifuged, by supernatant collection, chitosan mixing is added into supernatant, and be put into dehydration in vacuum freeze drier, obtains marine algae powder；The preparation of S5, strain fermentating liquid；S6, strain fermentating liquid is slowly added in marine algae powder, is constantly stirred evenly during addition and piles fermentation heap；S7, fermentation heap is overthrown to and is piled again new fermentation heap, form alga fertilizer after fermentation heap is spread out.The present invention handles Enteromorpha by the method for enzymolysis, can retain the active ingredients such as nutrient and the plant hormone in Enteromorpha to greatest extent, small to effective active composition destruction in Enteromorpha, does not influence the effect of later stage fertilizer.
Description
Technical field
The present invention relates to alga fertilizer technical fields more particularly to a kind of using Enteromorpha as the alga fertilizer preparation method of raw material.
Background technology
Frond grass green, tubulose film quality, grows thickly, and major branch is apparent, and branch is elongated, high up to 1 meter.Base portion is adhered to holdfast
On rock, it is grown in middle tide band beach, on chad.Due to Global climate change, body eutrophication etc., ocean is caused
Tangleweed Enteromorpha green tide is broken out.A large amount of Enteromorpha floatings gather bank, block the fairway, while destroying marine ecosystems, sternly
Inshore fishing, tourism development, most type marine products is threatened to be widely distributed in each ocean in the whole world again, some types are half
It is also shown in salt water or rivers.Be frequently grown on the rock of intertidal zone or Shi Zhaozhong or the chad on silt beach on, sometimes also can be attached
Life is on the frond of tangleweed.Currently, domestic alga fertilizer production technology mainly the methods of broken, chemical extraction using physics,
It is more to effective active composition destruction in seaweed, seriously affect the effect that post-production makes fertilizer.
Therefore, it is that the alga fertilizer preparation method of raw material is used to solve the above problems that we have proposed a kind of using Enteromorpha.
Invention content
The purpose of the present invention is to solve disadvantages existing in the prior art, and propose a kind of using Enteromorpha as raw material
Alga fertilizer preparation method.
It is a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, include the following steps：
S1, it air-dries, air-dried Enteromorpha is crushed using colloid mill, obtaining granularity is after cleaning up fresh Enteromorpha
The seaweed particle of 260-300 mesh；
S2, seaweed particle is used to distillation water dissolution, is constantly stirred during dissolving, and water temperature when dissolving is kept to exist
37 DEG C -42 DEG C, obtain seaweed colloidal solution；
S3, enzymolysis：Cellulase is added into seaweed colloidal solution, seaweed colloidal solution is constantly stirred, stirring 4
After h-6 h, continue to add 1,4 beta-glucanase, carbohydrase, alpha-amylase, beta amylase and α-maltose, and at 36 DEG C -37
3 h-4 h are stirred under conditions of DEG C, continuously add bromelain and mannuronic acid lyases, it is continuous to be mixed, and
3 h-4 h are stirred under conditions of 36 DEG C -37 DEG C obtains mixed liquor；
S4, mixed liquor is centrifuged, sediment is discarded after the completion of centrifugation, by supernatant collection, addition chitosan fills into supernatant
Divide mixing, and be put into dehydration in vacuum freeze drier, obtains marine algae powder；
The preparation of S5, strain fermentating liquid：By Bacillus cercus bacterium solution, Rhizopus oryzae bacterium solution, white-rot fungi bacterium solution and coarse arteries and veins
Born of the same parents' bacterium bacterium solution is proportionally mixed to get strain fermentating liquid；
S6, strain fermentating liquid is slowly added in marine algae powder, constantly stirs evenly and pile during addition
Fermentation heap, insertion long handle thermometer ferments in fermentation heap；
S7, when long handle thermometer shows the temperature of fermentation heap at 30 DEG C -50 DEG C, after 10 h-16 h of continuing fermentation, will ferment
Heap is overthrown and piles new fermentation heap again, overthrows to lay equal stress on after 5-7 days and newly piles new fermentation heap, repeats 4-6 times, by fermentation heap
Alga fertilizer is formed after spreading out.
Preferably, the process of the cleaning is to clean Enteromorpha 2-3 times using tap water, and the Enteromorpha after cleaning is immersed in
36 h-48 h in the ethyl alcohol of 95 % take out Enteromorpha and make 2-3 times wash with distilled water, Enteromorpha is air-dried under field conditions (factors).
Preferably, the final concentration of 1.5 g/L-2.0 g/L of the cellulase, final concentration of the 1.8 of 1,4 beta-glucanase
G/L-2.6 g/L, the final concentration of 1.4 g/L-2.2 g/L of carbohydrase, the final concentration of 1.5 g/L-2.0 g/ of alpha-amylase
L, the final concentration of 1.5 g/L-2.0 g/L of beta amylase, the final concentration of 1.5 g/L-2.0 g/L of α-maltose, pineapple
The final concentration of 0.8 g/L-1.5 g/L of protease, the final concentration of 1.5 g/L-2.0 g/L of mannuronic acid lyases.
Preferably, the rotating speed of the centrifugation is 400 rpm/min-600 rpm/min, and the time of centrifugation is 10 min-15
Min, vacuum degree when vacuum freeze drier is dehydrated are 20 Pa-30 Pa, and the temperature of dehydration is subzero 10 degree and arrives minus 8 degrees Centigrade,
The time of dehydration is 45 min-60 min.
Preferably, the strain fermentating liquid includes the raw material of following parts by weight：3-5 parts of bacillus subtilis bacterium solution, meter Gen
3-5 parts of 2-4 parts of mould bacterium solution, 1-3 parts of white-rot fungi bacterium solution and Pediococcus acidilactici bacterium solution.
Preferably, the strain fermentating liquid and the ratio of marine algae powder mixing are 1：（1000-1200）, chitosan and supernatant
The ratio of liquid is 1：（20-25）.
Preferably, during the fermentation the top of fermentation heap beat vertically downward 3-5 rows ferment hole, the two of fermentation heap
Side horizontal direction beats 1-2 rows fermentation hole, and the diameter in hole of fermenting is 4 cm-6 cm, and the width of fermentation heap is 60 cm-80 cm, hair
The height of ferment heap is 30 cm-50 cm.
The beneficial effects of the invention are as follows：
1, of the invention, by cleaning three times, the impurity on Enteromorpha can be washed off so that process of the Enteromorpha in subsequent production fertilizer
In will not carry hinder production ingredient, influence the normal production of fertilizer, the characteristic of alga fertilizer will not be reduced.
2, of the invention, Enteromorpha is handled by the method for enzymolysis, can retain to greatest extent nutrient in Enteromorpha and
The active ingredients such as plant hormone, it is small to effective active composition destruction in Enteromorpha, the effect of later stage fertilizer is not influenced.
3, of the invention, by using continuous accumulation, overthrows the method accumulated again and make the more thorough of fermentation so that is last
The nutritive value higher of obtained alga fertilizer, be also convenient for that follow-up crops can absorb when absorbing seawood meal more thoroughly.
Specific implementation mode
The present invention is made further to explain with reference to specific embodiment.
Embodiment one
It is a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, include the following steps：
S1, fresh Enteromorpha is cleaned 3 times using tap water, the Enteromorpha after cleaning is immersed in 40 h in the ethyl alcohol of 95 %, is taken
Go out Enteromorpha and make 3 times wash with distilled water, Enteromorpha is air-dried under field conditions (factors), air-dried Enteromorpha is carried out powder using colloid mill
It is broken, obtain the seaweed particle that granularity is 300 mesh；
S2, seaweed particle is used to distillation water dissolution, is constantly stirred during dissolving, and water temperature when dissolving is kept to exist
37 DEG C, obtain seaweed colloidal solution；
S3, enzymolysis：The cellulase of final concentration of 1.5 g/L is added into seaweed colloidal solution, seaweed colloidal solution is continuous
Be stirred, stir 4 h after, continue the 1,4 beta-glucanase for adding final concentration of 1.8 g/L, the sugar of final concentration of 1.4 g/L
Change enzyme, the alpha-amylase of final concentration of 1.5 g/L, the beta amylase of final concentration of 1.5 g/L and final concentration of 1.5 g/L-'s
α-maltose, and 3 h are stirred under conditions of 37 DEG C, bromelain and the end for continuously adding final concentration of 0.8 g/L are dense
Degree is the mannuronic acid lyases of 1.5 g/L, continuous to be mixed, and stirs 4 h under conditions of 36 DEG C and mixed
Liquid；
S4, by mixed liquor with rotating speed be 400 rpm/min, the time be 15 min under conditions of centrifuge, centrifugation after the completion of discard it is heavy
Starch, by supernatant collection, into 20 parts of supernatants, 1 part of chitosan of addition is sufficiently mixed, and it is 25 Pa's to be put into vacuum degree
45 min are dehydrated in vacuum freeze drier, the temperature of dehydration is subzero 10 degree, obtains marine algae powder；
The preparation of S5, strain fermentating liquid：By 3 parts of bacillus subtilis bacterium solution, 2 parts of Rhizopus oryzae bacterium solution, 1 part of white-rot fungi bacterium solution
It is proportionally mixed to get strain fermentating liquid for 3 parts with Pediococcus acidilactici bacterium solution；
S6,1 part of strain fermentating liquid is slowly added in 1000 parts of marine algae powder, is constantly stirred during addition
Fermentation heap is mixed uniformly and piled, makes a call to 5 rows fermentation hole vertically downward at the top of fermentation heap, the both sides horizontal direction of fermentation heap makes a call to 2
The diameter in row fermentation hole, hole of fermenting is 6 cm, and the width of fermentation heap is 60 cm, and the height of fermentation heap is 50 cm, in fermentation heap
Middle insertion long handle thermometer ferments；
S7, when long handle thermometer shows the temperature of fermentation heap at 40 DEG C, after 14 h of continuing fermentation, fermentation heap is overthrown and is laid equal stress on
New fermentation heap is newly piled, overthrows to lay equal stress on after 7 days and newly piles new fermentation heap, is repeated 5 times, forms sea after fermentation heap is spread out
Algae fertilizer.
Embodiment two
It is a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, include the following steps：
S1, fresh Enteromorpha is cleaned 3 times using tap water, the Enteromorpha after cleaning is immersed in 42 h in the ethyl alcohol of 95 %, is taken
Go out Enteromorpha and make 3 times wash with distilled water, Enteromorpha is air-dried under field conditions (factors), air-dried Enteromorpha is carried out powder using colloid mill
It is broken, obtain the seaweed particle that granularity is 300 mesh；
S2, seaweed particle is used to distillation water dissolution, is constantly stirred during dissolving, and water temperature when dissolving is kept to exist
37 DEG C, obtain seaweed colloidal solution；
S3, enzymolysis：The cellulase of final concentration of 1.8 g/L is added into seaweed colloidal solution, seaweed colloidal solution is continuous
Be stirred, stir 5 h after, continue the 1,4 beta-glucanase for adding final concentration of 2.2 g/L, the sugar of final concentration of 1.8 g/L
Change enzyme, the alpha-amylase of final concentration of 1.7 g/L, the α-of the beta amylase of final concentration of 1.8 g/L and final concentration of 1.7 g/L
Maltose, and 4 h are stirred under conditions of 37 DEG C, continuously add the bromelain and final concentration of final concentration of 1.2 g/L
It is continuous to be mixed for the mannuronic acid lyases of 1.8 g/L, and stir 4 h under conditions of 37 DEG C and mixed
Liquid；
S4, by mixed liquor with rotating speed be 500 rpm/min, the time be 15 min under conditions of centrifuge, centrifugation after the completion of discard it is heavy
Starch, by supernatant collection, into 23 parts of supernatants, 1 part of chitosan of addition is sufficiently mixed, and it is 30 Pa's to be put into vacuum degree
60 min are dehydrated in vacuum freeze drier, the temperature of dehydration is subzero 10 degree, obtains marine algae powder；
The preparation of S5, strain fermentating liquid：By 4 parts of bacillus subtilis bacterium solution, 3 parts of Rhizopus oryzae bacterium solution, 2 parts of white-rot fungi bacterium solution
With 4 parts of Pediococcus acidilactici bacterium solution, it is proportionally mixed to get strain fermentating liquid；
S6,1 part of strain fermentating liquid is slowly added in 1500 parts of marine algae powder, is constantly stirred during addition
Fermentation heap is mixed uniformly and piled, makes a call to 5 rows fermentation hole vertically downward at the top of fermentation heap, the both sides horizontal direction of fermentation heap makes a call to 2
The diameter in row fermentation hole, hole of fermenting is 6 cm, and the width of fermentation heap is 80 cm, and the height of fermentation heap is 50 cm, in fermentation heap
Middle insertion long handle thermometer ferments；
S7, when long handle thermometer shows the temperature of fermentation heap at 4 DEG C, after 14 h of continuing fermentation, fermentation heap is overthrown and again
New fermentation heap is piled, overthrows to lay equal stress on after 7 days and newly piles new fermentation heap, is repeated 5 times, forms seaweed after fermentation heap is spread out
Fertilizer.
Embodiment three
It is a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, include the following steps：
S1, fresh Enteromorpha is cleaned 3 times using tap water, the Enteromorpha after cleaning is immersed in 42 h in the ethyl alcohol of 95 %, is taken
Go out Enteromorpha and make 3 times wash with distilled water, Enteromorpha is air-dried under field conditions (factors), air-dried Enteromorpha is carried out powder using colloid mill
It is broken, obtain the seaweed particle that granularity is 260-300 mesh；
S2, seaweed particle is used to distillation water dissolution, is constantly stirred during dissolving, and water temperature when dissolving is kept to exist
37 DEG C, obtain seaweed colloidal solution；
S3, enzymolysis：The cellulase of final concentration of 2.0 g/L is added into seaweed colloidal solution, seaweed colloidal solution is continuous
Be stirred, stir 6 h after, continue the 1,4 beta-glucanase for adding final concentration of 2.6 g/L, the sugar of final concentration of 2.2 g/L
Change enzyme, the alpha-amylase of final concentration of 2.0 g/L, the α-of the beta amylase of final concentration of 2.0 g/L and final concentration of 2.0 g/L
Maltose, and 4 h are stirred under conditions of 37 DEG C, continuously add the bromelain and final concentration of final concentration of 1.5 g/L
It is continuous to be mixed for the mannuronic acid lyases of 2.0 g/L, and stir 4 h under conditions of 37 DEG C and mixed
Liquid；
S4, by mixed liquor with rotating speed be 500 rpm/min, the time be 15 min under conditions of centrifuge, centrifugation after the completion of discard it is heavy
Starch, by supernatant collection, into 25 parts of supernatants, 1 part of chitosan of addition is sufficiently mixed, and it is 20 Pa's to be put into vacuum degree
60 min are dehydrated in vacuum freeze drier, the temperature of dehydration is minus 8 degrees Centigrade, obtains marine algae powder；
The preparation of S5, strain fermentating liquid：By 5 parts of bacillus subtilis bacterium solution, 4 parts of Rhizopus oryzae bacterium solution, 3 parts of white-rot fungi bacterium solution
With 5 parts of Pediococcus acidilactici bacterium solution, it is proportionally mixed to get strain fermentating liquid；
S6,1 part of strain fermentating liquid is slowly added in 1200 parts of marine algae powder, is constantly stirred during addition
Fermentation heap is mixed uniformly and piled, makes a call to 5 rows fermentation hole vertically downward at the top of fermentation heap, the both sides horizontal direction of fermentation heap makes a call to 2
The diameter in row fermentation hole, hole of fermenting is 6 cm, and the width of fermentation heap is 80 cm, and the height of fermentation heap is 50 cm, in fermentation heap
Middle insertion long handle thermometer ferments；
S7, when long handle thermometer shows the temperature of fermentation heap at 50 DEG C, after 16 h of continuing fermentation, fermentation heap is overthrown and is laid equal stress on
New fermentation heap is newly piled, overthrows to lay equal stress on after 5 days and newly piles new fermentation heap, is repeated 6 times, forms sea after fermentation heap is spread out
Algae fertilizer.
It is identical to choose 4 pieces of soil properties, the melonry of area equation plants watermelon seedling, and the length of watermelon seedling on 4 plots of melonries
Gesture, planting number is identical with spacing distance, and it is control group that one plot of melonry thereto, which applies common farm manure, and thereto three
Block melonry applies by embodiment one, using Enteromorpha as the alga fertilizer of raw material made of the method for embodiment two and embodiment three, point
Not Wei experimental group one, experimental group two and experimental group three, and experimental group one, experimental group two, experimental group three and control group apply fertilizer
Time and weight it is all identical, experimental result is as follows：
| Experimental group one | Experimental group two | Experimental group three | Control group | |
| Embodiment one | Embodiment two | Embodiment three | Common farm manure | |
| Blade condition | It is excellent | It is excellent | It is excellent | It is good |
| The yield of watermelon | 2600 jin | 2800 jin | 2700 jin | 1980 jin |
From above-mentioned experimental result：Using Enteromorpha as the seaweed of raw material made of embodiment one, embodiment two and embodiment three
Fertilizer can significantly improve the yield of crop, and the effect of wherein embodiment two is best, therefore, embodiment second is that the present invention best reality
Apply example.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, which is characterized in that include the following steps：
S1, it air-dries, air-dried Enteromorpha is crushed using colloid mill, obtaining granularity is after cleaning up fresh Enteromorpha
The seaweed particle of 260-300 mesh；
S2, seaweed particle is used to distillation water dissolution, is constantly stirred during dissolving, and water temperature when dissolving is kept to exist
37 DEG C -42 DEG C, obtain seaweed colloidal solution；
S3, enzymolysis：Cellulase is added into seaweed colloidal solution, seaweed colloidal solution is constantly stirred, stirring 4
After h-6 h, continue to add 1,4 beta-glucanase, carbohydrase, alpha-amylase, beta amylase and α-maltose, and at 36 DEG C -37
3 h-4 h are stirred under conditions of DEG C, continuously add bromelain and mannuronic acid lyases, it is continuous to be mixed, and
3 h-4 h are stirred under conditions of 36 DEG C -37 DEG C obtains mixed liquor；
S4, mixed liquor is centrifuged, sediment is discarded after the completion of centrifugation, by supernatant collection, addition chitosan fills into supernatant
Divide mixing, and be put into dehydration in vacuum freeze drier, obtains marine algae powder；
The preparation of S5, strain fermentating liquid：By Bacillus cercus bacterium solution, Rhizopus oryzae bacterium solution, white-rot fungi bacterium solution and coarse arteries and veins
Born of the same parents' bacterium bacterium solution is proportionally mixed to get strain fermentating liquid；
S6, strain fermentating liquid is slowly added in marine algae powder, constantly stirs evenly and pile during addition
Fermentation heap, insertion long handle thermometer ferments in fermentation heap；
S7, when long handle thermometer shows the temperature of fermentation heap at 30 DEG C -50 DEG C, after 10 h-16 h of continuing fermentation, will ferment
Heap is overthrown and piles new fermentation heap again, overthrows to lay equal stress on after 5-7 days and newly piles new fermentation heap, repeats 4-6 times, by fermentation heap
Alga fertilizer is formed after spreading out.
2. according to claim 1 a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, which is characterized in that the cleaning
Process be by Enteromorpha using tap water clean 2-3 times, the Enteromorpha after cleaning is immersed in 36 h-48 h in the ethyl alcohol of 95 %,
It takes out Enteromorpha and makes 2-3 times wash with distilled water, Enteromorpha is air-dried under field conditions (factors).
3. according to claim 1 a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, which is characterized in that the fiber
The final concentration of 1.5 g/L-2.0 g/L of plain enzyme, the final concentration of 1.8 g/L-2.6 g/L of 1,4 beta-glucanase, the end of carbohydrase
A concentration of 1.4 g/L-2.2 g/L, the final concentration of 1.5 g/L-2.0 g/L of alpha-amylase, final concentration of the 1.5 of beta amylase
G/L-2.0 g/L, the final concentration of 1.5 g/L-2.0 g/L of α-maltose, final concentration of 0.8 g/L- of bromelain
1.5 g/L, the final concentration of 1.5 g/L-2.0 g/L of mannuronic acid lyases.
4. according to claim 1 a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, which is characterized in that the centrifugation
Rotating speed be 400 rpm/min-600 rpm/min, time of centrifugation is 10 min-15 min, vacuum freeze drier dehydration
When vacuum degree be 20 Pa-30 Pa, the temperature of dehydration is subzero 10 degree and arrives minus 8 degrees Centigrade, and the time of dehydration is 45 min-60
min。
5. according to claim 1 a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, which is characterized in that the strain
Zymotic fluid includes the raw material of following parts by weight：3-5 parts of bacillus subtilis bacterium solution, 2-4 parts of Rhizopus oryzae bacterium solution, white-rot fungi bacterium
3-5 parts of 1-3 parts of liquid and Pediococcus acidilactici bacterium solution.
6. according to claim 1 a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, which is characterized in that the strain
Zymotic fluid and the ratio of marine algae powder mixing are 1：（1000-1200）, the ratio of chitosan and supernatant is 1：（20-25）.
7. according to claim 1 a kind of using Enteromorpha as the alga fertilizer preparation method of raw material, which is characterized in that the fermentation
During beat 3-5 rows vertically downward at the top of fermentation heap and ferment hole, the both sides horizontal direction of fermentation heap beats 1-2 rows and ferments
The diameter in hole, hole of fermenting is 4 cm-6 cm, and the width of fermentation heap is 60 cm-80 cm, and the height of fermentation heap is 30 cm-50
cm。
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Cited By (7)
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| CN109734528A (en) * | 2019-03-25 | 2019-05-10 | 广西中连智浩科技有限公司 | A kind of preparation method of novel alga slow release fertilizer |
| CN110105137A (en) * | 2019-05-08 | 2019-08-09 | 广东丰康生物科技有限公司 | A kind of preparation method of excellent ease ultra micro magnetic mill alga fertilizer |
| CN110655424A (en) * | 2019-11-06 | 2020-01-07 | 中国农业科学院烟草研究所 | Preparation method and application of biofertilizer taking enteromorpha and burkholderia pyrrocinia as raw materials |
| CN112167268A (en) * | 2019-07-02 | 2021-01-05 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
| CN112794767A (en) * | 2021-02-25 | 2021-05-14 | 湖南开门子肥业有限公司 | Compound seaweed fertilizer and preparation method thereof |
| CN112790085A (en) * | 2021-01-14 | 2021-05-14 | 四川大学青岛研究院 | Method for preparing seedling culture substrate by utilizing waste cabbage leaves and enteromorpha |
| CN113133522A (en) * | 2021-05-28 | 2021-07-20 | 范龙 | Probiotics complex enzyme fermentation type enteromorpha nutritional supplement and preparation method and application thereof |
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Application publication date: 20180817 |