CN106167422A - The preparation method of a kind of compound marine alga fertilizer and compound marine alga fertilizer thereof - Google Patents
The preparation method of a kind of compound marine alga fertilizer and compound marine alga fertilizer thereof Download PDFInfo
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- CN106167422A CN106167422A CN201610535285.8A CN201610535285A CN106167422A CN 106167422 A CN106167422 A CN 106167422A CN 201610535285 A CN201610535285 A CN 201610535285A CN 106167422 A CN106167422 A CN 106167422A
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- sargassum
- marine alga
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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Abstract
The invention belongs to the technical field of alga fertilizer, be specifically related to the preparation method of a kind of compound marine alga fertilizer and compound marine alga fertilizer thereof.The preparation method of this kind of compound marine alga fertilizer, comprises the following steps: (1) pretreatment；(2) enzyme extraction；(3) microorganism is extracted；(4) prepared by fertilizer.The preparation method of described compound marine alga fertilizer retains the effective active composition of compound Sargassum to greatest extent, and substantially increases the extraction ratio of effective ingredient.
Description
Technical field
The invention belongs to the technical field of alga fertilizer, be specifically related to the preparation method of a kind of compound marine alga fertilizer and answer
Close marine alga fertilizer.
Background technology
Sargassum is that be grown in ocean low waits cryptogam, rich in proteins, aminoacid, inorganic salt, vitamin, brown
The biological active substanceies such as algin and a small amount of enzyme, phytohormone, polyphenol and polysaccharide.After biochemical treatment, as novel fertilizer
Material uses, and has volume increase, alleviates disease, reduces that medicine is residual, improve local flavor etc. acts on.
Sargassum is utilized to produce marine alga fertilizer, critical technological point mainly cellulose, pectic substance, protein etc. in solving Sargassum
On the basis of main component degraded, retain to greatest extent a great number of elements in Sargassum, trace element, cytokinin, gibberellins,
The effective ingredient such as the class basic element of cell division, polyphenolic substance and antibiotic agents.At present, domestic alga fertilizer production technology is mainly adopted
By methods such as broken, the chemical extractions of physics, more to effective active component damage in Sargassum, have a strong impact on post-production and make fertilizer
The effect of material.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of compound marine alga fertilizer and compound marine alga fertilizer thereof, described multiple
The preparation method closing marine alga fertilizer retains the effective active composition of compound Sargassum to greatest extent, and substantially increases effectively one-tenth
The extraction ratio divided.
For achieving the above object, the present invention is achieved by the following technical solutions:
The preparation method of a kind of compound marine alga fertilizer, comprises the following steps:
(1) pretreatment: first gather fresh wild Rhodomela confervoides, sugar Thallus Laminariae (Thallus Eckloniae), Sha Zao, car net algae and Fucus Vesiculosus, then anti-with clear water
Multiple cleaning, removes wherein impurity, cleans up and be mixed to get mixed algae, be 40-45% by mixed algae extrusion dehydration to water content；
At dehydration mixed algae is placed in-25 DEG C, freezing 4h, first uses tissue mashing crusher machine after taking out normal temperature unfreezing, then uses colloid mill
Pulverizing obtains mixed algae powder；Finally in mixed algae powder, add water mix and blend by material-water ratio 1:0.45 and make homogenate；
(2) enzyme extraction: be firstly added circumscribed beta-1,4 glucose enzyme and cellobiohydrolase at 45 DEG C, pH in gained is homogenized
Value is enzymolysis 6 hours under conditions of 5, then adds Isosorbide-5-Nitrae-β-mannuronic acid lyases and bromelain at 40 DEG C, pH
Value is enzymolysis 7 hours under conditions of 6；Be eventually adding saccharifying enzyme 50 DEG C, pH value be enzymolysis 3 hours under conditions of 4；Enzymolysis is complete
The homogenate become is performing centrifugal separation on obtaining Sargassum enzymolysis solution and Sargassum Production With Residue of Enzymatic Hydrolysis；
(3) microorganism is extracted: first add citrus peel residue, aspergillus niger and neurospora crassa in gained Sargassum enzymolysis solution 45
DEG C, pH value be to stir 4 hours under conditions of 5, be subsequently adding Bacillus cercus 50 DEG C, pH value be that to stir 6 under conditions of 4 little
Time；Finally it is performing centrifugal separation on obtaining Sargassum microorganism extracting solution and slag is extracted in Sargassum microorganism；
(4) prepared by fertilizer: through ultrafiltration, Sargassum microorganism extracting solution is prepared compounding sea polysaccharides concentrated solution, by many for compound Sargassum
Compounding sea polysaccharides fertilizer additive is prepared after sugar concentrated solution atomization drying；By Sargassum Production With Residue of Enzymatic Hydrolysis and the step of step (2) gained
(3) slag mixing is extracted in the Sargassum microorganism of gained, and adds sea mud and be stirred uniform post-drying pelletize and prepare compound Sargassum and have
Machine granular fertilizer.
Described step is crushed to 60-80 mesh in (1).
Described step is crushed to 200-250 mesh in (1).
In described step (2), the addition of circumscribed beta-1,4 glucose enzyme is 2.0-2.5g/L；Adding of cellobiohydrolase
Enter
Amount is 1.5-2.5g/L；The addition of 1,4-β-mannuronic acid lyases is 0.8-1.2 g/L；Adding of bromelain
Entering amount is 0.6-1.0 g/L；The addition of saccharifying enzyme is 1.5-2.0 g/L.
In described step (3), the addition of citrus peel residue is 4.0-6.0 g/L；The addition of aspergillus niger is 1.0-2.0g/
L；
The addition of neurospora crassa is 0.6-1.2g/L；The addition of Bacillus cercus is 1.0-1.5g/L.
In described step (3), citrus peel residue is 100-150 mesh.
In described step (4), the particle diameter of sea mud is 100-200 micron.
The temperature of described step (4) atomization drying is maintained at 60-75 DEG C.
Described step (4) is dried the temperature of pelletize and is maintained at 80-95 DEG C；Control particle diameter is 2-3mm.
Use the compound marine alga fertilizer that described preparation method prepares, including following raw material: Rhodomela confervoides, sugar Thallus Laminariae (Thallus Eckloniae), Sha Zao, car
Net algae, Fucus Vesiculosus, circumscribed beta-1,4 glucose enzyme, cellobiohydrolase, 1,4-β-mannuronic acid lyases, bromelain
Enzyme, saccharifying enzyme, citrus peel residue, aspergillus niger, neurospora crassa, Bacillus cercus and sea mud；Rhodomela confervoides the most by weight:
Sugar Thallus Laminariae (Thallus Eckloniae): Sha Zao: Che Wangzao: Fucus Vesiculosus is 2:7:3:11:5.
Compared with prior art, advantages of the present invention and good effect are:
1, pretreatment of the present invention, combines freezing according to precedence with broken, pulverizing so that alginic cell wall
Punctured by the ice crystal of intracellular formation, be not only effectively protected Sargassum effective active composition, and beneficially effective ingredient carries
Take the raising of rate so that the extraction ratio of follow-up effective ingredient improves 35-46%.
2, the present invention uses enzyme extraction to work in coordination with the mode that microorganism is extracted, and compound Sargassum carries out the extraction of effective ingredient.
Compound Sargassum is degraded by the mode first using multiple enzyme successively to match, and destroys the fibre structure cell wall of compound Sargassum,
Reach to remove the effect of the thin wall of Sargassum, made Sargassum effective ingredient discharge, and remain effective active composition in Sargassum well；
And the priority addition sequence between collocation and each collocation between different enzymes so that extraction ratio improves 25-41%.Then
Using microorganism to extract again to extract compound Sargassum targetedly, make activity group more expose, raising effect is imitated
Really.
The citrus peel residue collocation aspergillus niger simultaneously added during microorganism is extracted and coarse arteries and veins fertilizer bacterium, can produce
Pectase, cellulase, dimethylbenzene phenolase and xylanase, such that it is able to proceed enzyme extraction simultaneously, substantially increase and carry
Take rate.And containing substantial amounts of functional materials, such as essential oil, pectin, flavonoids, dietary fiber in citrus peel residue
Deng, the trophic component of fertilizer can be supplemented further.
3, the present invention has carried out appropriate process to the Sargassum extraction slag produced during extracting, and Sargassum is extracted slag and sea
Mud compounds, and sea mud includes organic matter 2.47%, salt 5.86%, copper 78PPm, zinc 116PPm, manganese 7.50PPm, sodium 2%, magnesium 2%, potassium
1.38%, calcium 3%, ferrum 0.51%, pH value 7.0.Help and add sea mud, not only enrich various trace element, fertilizer can be improved simultaneously
Utilization rate, reduce cost, it is achieved that comprehensive utilization of various nutritional labelings in double Sargassums.
Below the compound marine alga fertilizer using preparation method gained of the present invention is carried out manure trial.
1 materials and methods
1.1 for studying thing: Fructus Mali pumilae.
1.2 EXPERIMENTAL DESIGN:
Matched-pair design, plot area 40m2 are taked in test, and each test processes and repeats 4 times, peripheral hardware protection row.Each test
Point requires physical features smooth, and soil fertility is medium uniformly, has irrigation condition, and other cultivation management measure is consistent with land for growing field crops.
Arranging altogether at 2, be arranged in Yantai City at one, soil types is tide brown earth, and quality is light loam, topsoil 20cm
Soil organic matter content is 1.15 %, alkali-hydrolyzable nitrogen 67.8mg/kg, rapid available phosphorus 13.9mg/kg, available potassium 67.8mg/kg.
Being arranged in Weihai City at another, soil types is river moisture soil, and the soil texture is light earth, topsoil 20cm soil organic matter content
It is 1.20 %, alkali-hydrolyzable nitrogen 64.0mg/kg, rapid available phosphorus 15.5mg/kg, available potassium 65.7mg/kg.Test sets at 2
Reason, processes A: compound marine alga fertilizer.Compound Sargassum fertilizer amount is 3g/ community time, respectively at florescence, Fruit
Phase, coloring phase dilute 1500 times respectively and respectively spray 1 time.Process B (CK): conventional fertilizer application.
2 results and analysis
In Yantai City and Weihai City's soil Fructus Mali pumilae spray compound marine alga fertilizer per hectare relatively compare respectively volume increase 6749kg and
5128kg, rate of growth is respectively 18.2 % and 19.4 %, increases income respectively 5371 yuan and 7380 yuan.Check through t, in Yantai
In city and Weihai City's soil, Fructus Mali pumilae sprays compound Sargassum soil fertility quality A and all reaches pole significant level with compareing volume variance, and explanation is used
Compound marine alga fertilizer has extremely notable effect of increasing production.
Detailed description of the invention
Below in conjunction with detailed description of the invention, technical scheme is described in further detail.
The preparation method of a kind of compound marine alga fertilizer, comprises the following steps:
(1) pretreatment: first gather fresh wild Rhodomela confervoides, sugar Thallus Laminariae (Thallus Eckloniae), Sha Zao, car net algae and Fucus Vesiculosus, then anti-with clear water
Multiple cleaning, removes wherein impurity, cleans up and be mixed to get mixed algae, be 40-45% by mixed algae extrusion dehydration to water content；
At dehydration mixed algae is placed in-25 DEG C, freezing 4h, first uses tissue mashing machine to be crushed to 60-80 mesh after taking out normal temperature unfreezing, then
Use colloid mill to be crushed to 200-250 mesh and obtain mixed algae powder；The last material-water ratio 1:0.45 that presses in mixed algae powder adds
Water mix and blend makes homogenate；
(2) enzyme extraction: be firstly added 2.0-2.5g/L circumscribed beta-1,4 glucose enzyme and 1.5-2.5g/L fiber in gained is homogenized
Disaccharide-hydrolysing enzymes 45 DEG C, pH value be enzymolysis 6 hours under conditions of 5, then add 0.8-1.2 g/L Isosorbide-5-Nitrae-β-mannose
Aldehydic acid lyases and 0.6-1.0 g/L bromelain 40 DEG C, pH value be enzymolysis 7 hours under conditions of 6；It is eventually adding 1.5-
2.0 g/L saccharifying enzyme 50 DEG C, pH value be enzymolysis 3 hours under conditions of 4；The homogenate completed by enzymolysis is performing centrifugal separation on obtaining sea
Algae enzymolysis solution and Sargassum Production With Residue of Enzymatic Hydrolysis；
(3) microorganism is extracted: first add 4.0-6.0 g/L citrus peel residue, 1.0-2.0g/L in gained Sargassum enzymolysis solution black
Aspergillosis and 0.6-1.2g/L neurospora crassa 45 DEG C, pH value be to stir 4 hours under conditions of 5, be subsequently adding 1.0-1.5g/L
Bacillus cercus 50 DEG C, pH value be to stir 6 hours under conditions of 4；Finally it is performing centrifugal separation on obtaining Sargassum microorganism to extract
Slag is extracted in liquid and Sargassum microorganism；
(4) prepared by fertilizer: through ultrafiltration, Sargassum microorganism extracting solution is prepared compounding sea polysaccharides concentrated solution, by many for compound Sargassum
Compounding sea polysaccharides fertilizer additive is prepared after sugar concentrated solution atomization drying；By Sargassum Production With Residue of Enzymatic Hydrolysis and the step of step (2) gained
(3) slag mixing is extracted in the Sargassum microorganism of gained, and adds the sea mud that particle diameter is 100-200 micron and be stirred uniform post-drying
Pelletize prepares compound Sargassum organic granular fertilizer.
In described step (3), citrus peel residue is 100-150 mesh.
The temperature of described step (4) atomization drying is maintained at 60-75 DEG C.
Described step (4) is dried the temperature of pelletize and is maintained at 80-95 DEG C；Control particle diameter is 2-3mm.
Use the compound marine alga fertilizer that described preparation method prepares, including following raw material: Rhodomela confervoides, sugar Thallus Laminariae (Thallus Eckloniae), Sha Zao, car
Net algae, Fucus Vesiculosus, circumscribed beta-1,4 glucose enzyme, cellobiohydrolase, 1,4-β-mannuronic acid lyases, bromelain
Enzyme, saccharifying enzyme, citrus peel residue, aspergillus niger, neurospora crassa, Bacillus cercus and sea mud；Rhodomela confervoides the most by weight:
Sugar Thallus Laminariae (Thallus Eckloniae): Sha Zao: Che Wangzao: Fucus Vesiculosus is 2:7:3:11:5.
Claims (10)
1. the preparation method of a compound marine alga fertilizer, it is characterised in that comprise the following steps:
(1) pretreatment: first gather fresh wild Rhodomela confervoides, sugar Thallus Laminariae (Thallus Eckloniae), Sha Zao, car net algae and Fucus Vesiculosus, then anti-with clear water
Multiple cleaning, removes wherein impurity, cleans up and be mixed to get mixed algae, be 40-45% by mixed algae extrusion dehydration to water content；
At dehydration mixed algae is placed in-25 DEG C, freezing 4h, first uses tissue mashing crusher machine after taking out normal temperature unfreezing, then uses colloid mill
Pulverizing obtains mixed algae powder；Finally in mixed algae powder, add water mix and blend by material-water ratio 1:0.45 and make homogenate；
(2) enzyme extraction: be firstly added circumscribed beta-1,4 glucose enzyme and cellobiohydrolase at 45 DEG C, pH in gained is homogenized
Value is enzymolysis 6 hours under conditions of 5, then adds Isosorbide-5-Nitrae-β-mannuronic acid lyases and bromelain at 40 DEG C, pH
Value is enzymolysis 7 hours under conditions of 6；Be eventually adding saccharifying enzyme 50 DEG C, pH value be enzymolysis 3 hours under conditions of 4；Enzymolysis is complete
The homogenate become is performing centrifugal separation on obtaining Sargassum enzymolysis solution and Sargassum Production With Residue of Enzymatic Hydrolysis；
(3) microorganism is extracted: first add citrus peel residue, aspergillus niger and neurospora crassa in gained Sargassum enzymolysis solution 45
DEG C, pH value be to stir 4 hours under conditions of 5, be subsequently adding Bacillus cercus 50 DEG C, pH value be that to stir 6 under conditions of 4 little
Time；Finally it is performing centrifugal separation on obtaining Sargassum microorganism extracting solution and slag is extracted in Sargassum microorganism；
(4) prepared by fertilizer: through ultrafiltration, Sargassum microorganism extracting solution is prepared compounding sea polysaccharides concentrated solution, by many for compound Sargassum
Compounding sea polysaccharides fertilizer additive is prepared after sugar concentrated solution atomization drying；By Sargassum Production With Residue of Enzymatic Hydrolysis and the step of step (2) gained
(3) slag mixing is extracted in the Sargassum microorganism of gained, and adds sea mud and be stirred uniform post-drying pelletize and prepare compound Sargassum and have
Machine granular fertilizer.
It is combined the preparation method of marine alga fertilizer the most according to claim 1, it is characterised in that described step is crushed in (1)
60-80 mesh.
It is combined the preparation method of marine alga fertilizer the most according to claim 2, it is characterised in that described step is crushed in (1)
200-250 mesh.
It is combined the preparation method of marine alga fertilizer the most according to claim 1, it is characterised in that circumscribed β in described step (2)-
The addition of 1,4 glucanase is 2.0-2.5g/L；The addition of cellobiohydrolase is 1.5-2.5g/L；1,4-β-manna
The addition of alduronic acid lyases is 0.8-1.2 g/L；The addition of bromelain is 0.6-1.0 g/L；Adding of saccharifying enzyme
Entering amount is 1.5-2.0 g/L.
It is combined the preparation method of marine alga fertilizer the most according to claim 4, it is characterised in that peel of Citrus reticulata Blanco in described step (3)
The addition of slag is 4.0-6.0 g/L；The addition of aspergillus niger is 1.0-2.0g/L；The addition of neurospora crassa is 0.6-
1.2g/L；The addition of Bacillus cercus is 1.0-1.5g/L.
It is combined the preparation method of marine alga fertilizer the most according to claim 1, it is characterised in that peel of Citrus reticulata Blanco in described step (3)
Slag is 100-150 mesh.
It is combined the preparation method of marine alga fertilizer the most according to claim 1, it is characterised in that sea mud in described step (4)
Particle diameter is 100-200 micron.
It is combined the preparation method of marine alga fertilizer the most according to claim 1, it is characterised in that described step (4) atomization drying
Temperature be maintained at 60-75 DEG C.
It is combined the preparation method of marine alga fertilizer the most according to claim 1, it is characterised in that described step (4) dries pelletize
Temperature be maintained at 80-95 DEG C；Control particle diameter is 2-3mm.
10. one kind uses the compound marine alga fertilizer that preparation method described in claim 1 prepares, it is characterised in that include following former
Material: Rhodomela confervoides, sugar Thallus Laminariae (Thallus Eckloniae), Sha Zao, Che Wangzao, Fucus Vesiculosus, circumscribed beta-1,4 glucose enzyme, cellobiohydrolase, 1,4-β-sweet
Dew alduronic acid lyases, bromelain, saccharifying enzyme, citrus peel residue, aspergillus niger, neurospora crassa, Bacillus cercus and sea
Mud；Rhodomela confervoides the most by weight: sugar Thallus Laminariae (Thallus Eckloniae): Sha Zao: Che Wangzao: Fucus Vesiculosus is 2:7:3:11:5.
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| CN106748195A (en) * | 2016-12-28 | 2017-05-31 | 烟台泓源生物肥料有限公司 | A kind of fertilizer and its preparation technology containing natural plants growth substance |
| CN107298601A (en) * | 2017-05-16 | 2017-10-27 | 中国科学院烟台海岸带研究所 | A kind of preparation method of compound marine alga fertilizer |
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| CN107298601A (en) * | 2017-05-16 | 2017-10-27 | 中国科学院烟台海岸带研究所 | A kind of preparation method of compound marine alga fertilizer |
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Application publication date: 20161130 |