CN108383559A - A kind of method that microbial fermentation enzymolysis prepares organic liquid seaweed extract - Google Patents
A kind of method that microbial fermentation enzymolysis prepares organic liquid seaweed extract Download PDFInfo
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- CN108383559A CN108383559A CN201810299673.XA CN201810299673A CN108383559A CN 108383559 A CN108383559 A CN 108383559A CN 201810299673 A CN201810299673 A CN 201810299673A CN 108383559 A CN108383559 A CN 108383559A
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- seaweed
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
Abstract
The invention discloses a kind of microbial fermentations to digest the method for preparing organic liquid seaweed extract, belongs to field of fertilizer manufacturing.The present invention is using complex enzyme hydrolysis technology and microbial fermentation technology degradation seaweed, the complicated enzyme system generated by microbial fermentation and the complex enzyme comprising protease, cellulase, pectase, polysaccharide degrading enzyme etc. gradually degrade to seaweed, remove alginic cell wall, the contents such as alginic acid, polysaccharide, plant hormone are made fully to discharge, the decomposition that alginic acid, Plant growth-promoting effect factor isoreactivity substance are avoided under mild process conditions, remains the effective active composition in seaweed to the maximum extent.Energy-saving and water-saving low-carbon environment-friendly of the present invention, gained seaweed bio organic fertilizer is rich in the active ingredients such as a great number of elements, the trace element needed for abundant minerals and plant growth, soil texture can be improved to conserve water and soil, promote the growth of plant rhizosphere microbe, the yield using crop is significantly improved, there is huge market potential.
Description
Technical field
The present invention relates to a kind of microbial fermentations to digest the method for preparing organic liquid seaweed extract, belongs to field of fertilizer manufacturing.
Background technology
China is large agricultural country, but chemical fertilizer input used in traditional agriculture causes the wasting of resources for a long time
With and environmental pollution, keep soil hardening deterioration degree increasingly severe, the yield and quality of crops but reduces instead, therefrom
The agricultural product residue problem come has threatened the health of compatriots.China or the country of a water resources shortage, there is 4.2 hundred million mu
Arable land belongs to Dryland and half Dryland, and common chemical fertilizer uses and is difficult to solve the problems, such as that these are urgent, fundamentally to solve
The certainly unsustainable sex chromosome mosaicism in China's agricultural production, key measure are development of new plant nutriment regulator, reduce chemical fertilizer
Input, improves the utilization rate of fertilizer, improves the absorption rate and anti-adversity of Plant To Nutrient.
There is abundant marine algae resource in China, contains a large amount of non-nitrogen containing organic matter, potassium, calcium, magnesium, zinc, iodine etc. 40 in seaweed
Specific algal polysaccharides, alginic acid, height unsaturated fat in remaining kind of mineral matter element and abundant vitamin and seaweed
Sour and a variety of natural plants growth regulators have very high bioactivity.A kind of novel life of the alga fertilizer as environment-friendly high-efficiency
State fertilizer can meet fertilizer and pesticide in agricultural production and subtract and apply and ensure grain high yield and a variety of need such as environmentally friendly simultaneously
It asks, it is with the obvious advantage compared with chemical fertilizer, increasingly received by vast farmers, extensive market, potentiality to be exploited are huge.It grinds simultaneously
High-efficiency high-quality alga fertilizer is sent out, meets the development strategy that country greatly develops marine economy, is also agricultural production and income, solve soil
Pollution problem provides powerful guarantee.
Domestic and international more ripe alga fertilizer production technology is mainly broken, chemical extraction method using physics at present.Change
Extraction is to make cell resolution or endogenous substance solubilising, this method operation letter using acid, alkali and organic solvent processing alginic cell
It is single easy to implement, but chemical reagent is very big to the active material destruction in alginic cell, seriously affects post-production and makes fertilizer
Effect, remaining reagent also be easy potential hazard is caused to environment.The method of physics extraction is mainly cold using high pressure low temperature
But technology is crushed alginic cell wall, harsh to instrument and equipment requirement, and cost is higher, it is difficult to realize large-scale production.Biological enzymolysis
Method is small point that seaweed macromolecular substances are degraded to that plant is easy to be absorbed and utilized by a variety of enzymes for being generated using microbial fermentation
Sub- substance, it is pollution-free that this method working condition is mild, product safety and environmental protection has no toxic side effect, and can retain to the maximum extent
Bioactive ingredients in seaweed and nutriment are ideal seaweed fertilizer production methods.Alga fertilizer is produced using seaweed
While the critical technological point of material is the main components such as cellulose, pectic substance, protein in seaweed of degrading as possible, to the maximum extent
Retain cytokinin, gibberellin, the class basic element of cell division, polyphenolic substance, antibiotic agents and plant growth institute in seaweed
Need the activity of the active ingredients such as a great number of elements, trace element.But the enterprise of the seaweed production marine alga fertilizer of bioanalysis degradation at present
The mode of soda acid degradation can be aided with simultaneously mostly, there are different degrees of destructions to its active nutrients.
Invention content
To solve the deficiencies in the prior art, the present invention is quasi- extra large using complex enzyme hydrolysis technology and microbial fermentation technology degradation
Algae, the complicated enzyme system generated by microbial fermentation and complex enzyme comprising protease, cellulase, pectase etc. to seaweed into
Row is gradually degraded, and is removed alginic cell wall, so that the contents such as alginic acid, polysaccharide, plant hormone is fully discharged, while avoiding seaweed
The decomposition of the substances such as acid retains the effective active composition in seaweed.
The first purpose of the invention is to provide a kind of microbial fermentations to digest the method for preparing organic seaweed liquid fertilizer,
It the described method comprises the following steps：
(1) pretreatment of seaweed：New fresh seaweed raw material crosses water cleaning, removes the grains of sand and other impurities；
(2) water of 1-3 times of seaweed quality, crushing grinding is added in the seaweed after cleaning；
(3) seaweed slurries are added in fermentation tank, the microbial fermentation inoculum of addition seaweed quality 0.3-0.8%, stirring
It is heated to 20-40 DEG C after uniformly, heat-preservation fermentation 36-48h；
(4) complex enzyme formulation that seaweed quality 0.5-1.2% is added in the seaweed slurries after fermented, stirs evenly
After be heated to 40-60 DEG C, heat preservation enzymolysis 3h；
(5) solution after enzymolysis is filtered into obtain supernatant, concentrated to get organic liquid seaweed extract.
In one embodiment of the invention, the complex enzyme formulation includes 250,000 u/g of neutral proteinase, acidic protein
100,000 U/g of enzyme, 500,000 u/g of alkali protease, 200,000 u/g of zytase, 10,000 u/g of cellulase, 30,000 u/g of pectase.
In one embodiment of the invention, the seaweed includes one in kelp, undaria pinnitafida, Enteromorpha, asparagus
Kind or several mixtures.
In one embodiment of the invention, microbial fermentation inoculum be bacillus licheniformis, bacillus amyloliquefaciens,
The additive amount of the mixture of one or both of bacillus subtilis, microbial bacterial agent is 1~5 × 108CFU/g seaweed.
In one embodiment of the invention, the complex enzyme formulation is acid protease, neutral proteinase, alkalinity
The mixture of one or both of protease, pectase, cellulase, zytase, dextranase or amylase.
In one embodiment of the invention, the complex enzyme formulation is acid protease and cellulase, enzyme amount
The ratio between be 2 ︰ 1.
In one embodiment of the invention, the complex enzyme formulation be acid protease and pectase, enzyme amount it
Than for 1 ︰ 1.
In one embodiment of the invention, the complex enzyme formulation is pectase and cellulase, the ratio between enzyme amount
For 3 ︰ 1.
Second object of the present invention is to provide the liquid fertilizer that the method prepares.
Beneficial effects of the present invention are as follows：
(1) the targeted seaweed of the present invention includes mainly tangleweed kelp and the undaria pinnitafida of Phaeophyta, the dragon of Rhodophyta
Dish etc. is used as raw materials for production with one of which or several mixtures after cleaned crushing homogenate.Wherein kelp is as China master
The algae culturing kind wanted, distribution is wide, and yield is huge, comprehensively utilizes kelp product, and increasing its productive value, to be also pole have market
Potentiality.
(2) a kind of method that microbial fermentation enzymolysis prepares seaweed liquid fertilizer of present invention offer, production process low power consuming,
Pollution-free, simple for process without discharging, cost reduces by 30% or so than physical-chemical process, and meets environmental protection policy and sustainable development
Policy has good Social benefit and economic benefit.
(3) present invention is generated using complex enzyme hydrolysis technology and microbial fermentation technology degradation seaweed by microbial fermentation
Complicated enzyme system and complex enzyme comprising protease, cellulase, pectase, polysaccharide degrading enzyme etc. seaweed is gradually dropped
Solution, remove alginic cell wall, make alginic acid, polysaccharide, plant hormone (heteroauxin, isopentenyladenosine, isopentennyladenine, instead
Formula ribosylzeatin, abscisic acid, phenylacetic acid, salicylic acid, gibberellin and jasmonic) etc. contents fully discharge, avoid simultaneously
The destruction of highly basic, high temperature, high pressure avoids alginic acid, Plant growth-promoting effect factor isoreactivity object under mild process conditions
The decomposition of matter remains the effective active composition in seaweed to the maximum extent.
(4) seaweed bio organic fertilizer produced by the invention is rich in abundant minerals, fucoidin and plant growth institute
The active ingredients such as a great number of elements, the trace element needed, can promote growth and development and the mineral absorption of root system of plant, can promote to plant
Strain growth improves photosynthetic efficiency and increases fruit yield, resists poor environment stress and pest and disease damage, improves stress resistance of plant, and promote
Into the growth of plant rhizosphere microbe, improves soil texture and conserve water and soil.The antibacterial suppression worm effect of marine algae extract is killed with chemistry
Worm agent is suitable, is expected to substitute chemical pesticide in the future, there is huge market potential.
Specific implementation mode
In the following specific embodiments, enzyme preparation is all made of commercial enzyme preparation, wherein 250,000 u/g of neutral proteinase,
Acid protease (100,000 U/g), 500,000 u/g of alkali protease, 200,000 u/g of zytase, 10,000 u/g of cellulase, pectase 3
Ten thousand u/g.
Embodiment 1：
A kind of method that microbial fermentation enzymolysis prepares organic seaweed liquid fertilizer, specific preparation process are as follows：
(1) Fresh Laminaria Japonica raw material crosses water cleaning, removes the grains of sand and other impurities；
(2) the pure fresh water for accounting for 1.2 times of kelp quality, crushing grinding are added in the kelp 50g after cleaning；
(3) broken rear kelp slurries are added in round, addition 2.5 × 109The bacillus subtilis of CFU and 2.5
×109The bacillus amyloliquefaciens (the ratio between bacterium amount is 1 ︰ 1) of CFU, are heated to 37 DEG C, heat-preservation fermentation 48h after stirring evenly；
(4) the kelp slurries after fermented become brown from green, and detection pH value is less than 6, adds 0.6g acid wherein
Property protease and cellulase (2 ︰ 1), 55 DEG C are heated to after stirring evenly, heat preservation enzymolysis 3h；
(5) solution after enzymolysis is filtered into obtain supernatant, concentrated to get organic liquid seaweed extract.
Embodiment 2：
A kind of method that microbial fermentation enzymolysis prepares organic seaweed liquid fertilizer, specific preparation process are as follows：
(1) Fresh Laminaria Japonica and undaria pinnitafida (3 ︰ 1) raw material cross water cleaning, remove the grains of sand and other impurities；
(2) the pure fresh water for accounting for 2 times of seaweed quality, crushing grinding are added in the mixing seaweed 50g after cleaning；
(3) seaweed slurries are added in round, addition 2.5 × 109The bacillus subtilis of CFU and 2.5 × 109CFU
Bacillus licheniformis (the ratio between bacterium amount be 1 ︰ 1), 37 DEG C are heated to after stirring evenly, heat-preservation fermentation 48h；
(4) acid protease and pectase (1 ︰ 1) that 0.6g is added in the seaweed slurries after fermented, stir evenly
After be heated to 55 DEG C, heat preservation enzymolysis 3h；
(5) solution after enzymolysis is filtered into obtain supernatant, concentrated to get organic liquid seaweed extract.
Embodiment 3：
A kind of method that microbial fermentation enzymolysis prepares organic seaweed liquid fertilizer, specific preparation process are as follows：
(1) Fresh Laminaria Japonica and Enteromorpha (3 ︰ 1) raw material cross water cleaning, remove the grains of sand and other impurities；
(2) the pure fresh water for accounting for 3 times of seaweed quality, crushing grinding are added in the mixing seaweed 50g after cleaning；
(3) seaweed slurries are added in round, addition 4 × 109The bacillus amyloliquefaciens of CFU and 4 × 109CFU's
Bacillus licheniformis (the ratio between bacterium amount is 1 ︰ 1), is heated to 37 DEG C, heat-preservation fermentation 48h after stirring evenly；
(4) pectase and cellulase (3 ︰ 1) that 0.5g seaweed quality is added in the seaweed slurries after fermented, are stirred
55 DEG C are heated to after mixing uniformly, heat preservation enzymolysis 3h；
(5) solution after enzymolysis is filtered into obtain supernatant, concentrated to get organic liquid seaweed extract.
According to《Plant hormone Physico-chemical tests method》Composition detection is carried out to case study on implementation 1-3, the results are shown in Table 1.
Active component content in liquid seaweed extract prepared by the different embodiments of table 1
Embodiment 4
Liquid seaweed extract is prepared according to embodiment 3.
It is more uniform to choose growing way, strawberry, rice, grape, the soybean of robust growth
Experimental method：Foliar spray sprays anyway in plant leaf, primary in spray in bloom stage, and 1000 times of finished product is diluted when use,
Sprinkling of bearing fruit is primary, and when use dilutes 600 times or so, the repetition 2~3 times of 10~15 days interval times；
Soybean, four plants of rice are a duplicate block, and two plants of strawberry is a duplicate block, and one plant of grape is duplicate block.
Statistical experiment：It takes and takes hundred in duplicate block after soybean mature, take 2 in duplicate block after Strawberry ripening, grape maturity
Take 2 strings in duplicate block afterwards, after rice maturation duplicate block take four fringes take respectively four times take mean value after use pol at ambient temperature
Instrument and balance survey pol value, quality respectively.
The results are shown in table below：
Come as can be seen from the above table, has used the liquid seaweed extract example three of this patent to be compared with clear water, can not only carry
The sugariness of high fruit can also improve yield.
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not limited to the present invention, any to be familiar with this skill
The people of art can do various change and modification, therefore the protection model of the present invention without departing from the spirit and scope of the present invention
Enclosing be subject to what claims were defined.
Claims (9)
1. a kind of method preparing organic seaweed liquid fertilizer, which is characterized in that crush seaweed raw material plus seaweed slurry is made in water
Liquid, then microbe inoculation in seaweed slurries is fermented, it is digested after fermentation using complex enzyme formulation, and to enzymolysis
Liquid afterwards is filtered, concentrates.
2. according to the method described in claim 1, it is characterized in that, the described method comprises the following steps：
(1) pretreatment of seaweed：New fresh seaweed raw material crosses water cleaning, removes the grains of sand and other impurities；
(2) water of 1-3 times of seaweed quality, crushing grinding is added in the seaweed after cleaning；
(3) seaweed slurries are added in fermentation tank, the microbial fermentation inoculum of addition seaweed quality 0.3-0.8% stirs evenly
After be heated to 20-40 DEG C, heat-preservation fermentation 36-48h；
(4) complex enzyme formulation that seaweed quality 0.5-1.2% is added in the seaweed slurries after fermented, adds after stirring evenly
Heat is to 40-60 DEG C, heat preservation enzymolysis 3h；
(5) solution after enzymolysis is filtered into obtain supernatant, concentrated to get organic liquid seaweed extract.
3. according to the method described in claim 1, it is characterized in that, the complex enzyme formulation contains acid protease, neutral egg
At least two in white enzyme, alkali protease, pectase, cellulase, zytase, dextranase or amylase.
4. according to the method described in claim 1, it is characterized in that, the seaweed includes kelp, undaria pinnitafida, Enteromorpha, dragon
The mixture of one or more of dish.
5. according to the method described in claim 1, it is characterized in that, microbial fermentation inoculum is bacillus licheniformis, solution starch
The additive amount of the mixture of one or both of bacillus, bacillus subtilis, microbial bacterial agent is 1~5 × 108CFU/
G seaweed.
6. according to the method described in claim 3, it is characterized in that, the complex enzyme formulation is acid protease and cellulose
Enzyme, enzyme mass ratio are 2 ︰ 1；Or acid protease and pectase, enzyme mass ratio are 1 ︰ 1；Or pectase and cellulose
Enzyme, enzyme mass ratio are 3 ︰ 1.
7. the organic liquid seaweed extract prepared using any the method for claim 1~6.
8. application of organic liquid seaweed extract in field of agricultural cultivation described in claim 7.
9. a kind of breeding method of plant, which is characterized in that cultivate plant using organic liquid seaweed extract described in claim 7；
The plant includes crops, water fruits and vegetables；Organic liquid seaweed extract acts on plant by way of spraying or irrigating.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109020666A (en) * | 2018-07-18 | 2018-12-18 | 威海市世代海洋生物科技股份有限公司 | Kelp digests liquid fertilizer and preparation method thereof, application method |
| CN109207544A (en) * | 2018-11-05 | 2019-01-15 | 福建师范大学 | A kind of preparation method of chlorella antioxidation polypeptide |
| CN109369270A (en) * | 2018-11-26 | 2019-02-22 | 烟台富康生物科技有限公司 | A kind of environment-friendly fertilizer and its preparation process containing functional components of chlorella vulgaris |
| CN109369819A (en) * | 2018-10-22 | 2019-02-22 | 温州科技职业学院 | A kind of extracting method of Hijiki polysaccharide |
| CN109704858A (en) * | 2019-01-21 | 2019-05-03 | 李永兴 | A kind of method of temperate condition production alga fertilizer |
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| CN110105137A (en) * | 2019-05-08 | 2019-08-09 | 广东丰康生物科技有限公司 | A kind of preparation method of excellent ease ultra micro magnetic mill alga fertilizer |
| CN110183266A (en) * | 2019-04-17 | 2019-08-30 | 青岛明月蓝海生物科技有限公司 | A kind of small molecule alginic acid microbial manure |
| CN110372443A (en) * | 2019-07-26 | 2019-10-25 | 姚力楠 | A kind of preparation method of the bio-organic fertilizer of total nutrient liquid containing seaweed |
| CN111253179A (en) * | 2020-01-16 | 2020-06-09 | 佛山市安笑然生物技术有限公司 | Method for preparing functional seaweed fertilizer by co-fermenting seaweed with multiple strains |
| CN114164240A (en) * | 2021-09-08 | 2022-03-11 | 新洋丰农业科技股份有限公司 | Preparation method of seaweed extract by segmented enzymolysis |
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| CN115337329A (en) * | 2022-08-24 | 2022-11-15 | 中国海洋大学 | Preparation method of sargassum thunbergii polyphenol |
| CN115466142A (en) * | 2022-08-24 | 2022-12-13 | 烟台富慧农业科技有限公司 | Preparation process of nutrient-rich seaweed fertilizer |
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| CN109020666A (en) * | 2018-07-18 | 2018-12-18 | 威海市世代海洋生物科技股份有限公司 | Kelp digests liquid fertilizer and preparation method thereof, application method |
| CN109369819A (en) * | 2018-10-22 | 2019-02-22 | 温州科技职业学院 | A kind of extracting method of Hijiki polysaccharide |
| CN109207544B (en) * | 2018-11-05 | 2021-12-17 | 福建师范大学 | Preparation method of chlorella antioxidant polypeptide |
| CN109207544A (en) * | 2018-11-05 | 2019-01-15 | 福建师范大学 | A kind of preparation method of chlorella antioxidation polypeptide |
| CN109369270A (en) * | 2018-11-26 | 2019-02-22 | 烟台富康生物科技有限公司 | A kind of environment-friendly fertilizer and its preparation process containing functional components of chlorella vulgaris |
| WO2020107587A1 (en) * | 2018-11-26 | 2020-06-04 | 烟台富康生物科技有限公司 | Environmentally-friendly fertilizer containing chlorella functional components and preparation process therefor |
| CN109704826A (en) * | 2019-01-08 | 2019-05-03 | 苏州瑞禾生物科技有限公司 | Plant culture active water and preparation method thereof |
| CN109704858A (en) * | 2019-01-21 | 2019-05-03 | 李永兴 | A kind of method of temperate condition production alga fertilizer |
| CN110183266A (en) * | 2019-04-17 | 2019-08-30 | 青岛明月蓝海生物科技有限公司 | A kind of small molecule alginic acid microbial manure |
| CN110105137A (en) * | 2019-05-08 | 2019-08-09 | 广东丰康生物科技有限公司 | A kind of preparation method of excellent ease ultra micro magnetic mill alga fertilizer |
| CN110372443A (en) * | 2019-07-26 | 2019-10-25 | 姚力楠 | A kind of preparation method of the bio-organic fertilizer of total nutrient liquid containing seaweed |
| CN111253179A (en) * | 2020-01-16 | 2020-06-09 | 佛山市安笑然生物技术有限公司 | Method for preparing functional seaweed fertilizer by co-fermenting seaweed with multiple strains |
| CN114164240A (en) * | 2021-09-08 | 2022-03-11 | 新洋丰农业科技股份有限公司 | Preparation method of seaweed extract by segmented enzymolysis |
| CN114164240B (en) * | 2021-09-08 | 2022-11-25 | 新洋丰农业科技股份有限公司 | Preparation method of seaweed extract by segmented enzymolysis |
| CN115337329A (en) * | 2022-08-24 | 2022-11-15 | 中国海洋大学 | Preparation method of sargassum thunbergii polyphenol |
| CN115466142A (en) * | 2022-08-24 | 2022-12-13 | 烟台富慧农业科技有限公司 | Preparation process of nutrient-rich seaweed fertilizer |
| CN115160040A (en) * | 2022-09-08 | 2022-10-11 | 山东嘉禾海洋生物科技有限公司 | Alginic acid water-soluble fertilizer and preparation method thereof |
| CN115197018A (en) * | 2022-09-08 | 2022-10-18 | 山东嘉禾海洋生物科技有限公司 | Organic water-soluble fertilizer and preparation method thereof |
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