CN110372443A - A kind of preparation method of the bio-organic fertilizer of total nutrient liquid containing seaweed - Google Patents
A kind of preparation method of the bio-organic fertilizer of total nutrient liquid containing seaweed Download PDFInfo
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- CN110372443A CN110372443A CN201910681144.0A CN201910681144A CN110372443A CN 110372443 A CN110372443 A CN 110372443A CN 201910681144 A CN201910681144 A CN 201910681144A CN 110372443 A CN110372443 A CN 110372443A
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- seaweed
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- kelp
- organic fertilizer
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- 241001474374 Blennius Species 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 title claims abstract description 11
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 11
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 6
- 238000002360 preparation method Methods 0.000 title claims abstract description 4
- 241000512259 Ascophyllum nodosum Species 0.000 claims abstract description 11
- 239000004365 Protease Substances 0.000 claims abstract description 10
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 8
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
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- 238000006243 chemical reaction Methods 0.000 claims description 9
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- 239000003513 alkali Substances 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 3
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- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
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- 244000061456 Solanum tuberosum Species 0.000 description 1
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- 235000009184 Spondias indica Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
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- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
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- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- 235000008529 Ziziphus vulgaris Nutrition 0.000 description 1
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 244000000013 helminth Species 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- -1 phenols poly-compounds Chemical class 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
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- 229920001184 polypeptide Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
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- 230000035484 reaction time Effects 0.000 description 1
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- 235000009566 rice Nutrition 0.000 description 1
- 239000003516 soil conditioner Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
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- 235000013311 vegetables Nutrition 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
A kind of preparation method of the bio-organic fertilizer of total nutrient liquid containing seaweed, belongs to biological enzymolysis technology field.Key step is as follows: kelp being pre-processed, the multinomial seaweed active materials such as multiplex-enzyme extraction algal polysaccharides are added.Compound protease is added by the Viability peptide of protein digestion and amino acid in kelp, enriches seaweed activated species.Most have and is compounded with bacillus subtilis bacterium solution, the General Promotion content of organic matter.
Description
Technical field
The present invention relates to applied technical field in agriculture and forestry, contain seaweed it is particularly applicable to multiple kinds of crops and landscape flower
The liquid organic fertilizer of full nutrition.
Background technique
Grain of the fertilizer as nutrition needed for supply plant, it has also become the important foundation of modern agricultural development.At present with nitrogen
A large amount of uses of conventional composite fertilizer based on phosphorus potassium cause more serious soil hardening and environmental pollution.And it is such
Although chemical fertilizer plays certain good effect to increasing crop yield, produce little effect for promoting crops quality, Bu Nengman
The demand that sufficient China's consumption quality is increasingly promoted, and also create the inconsistent phenomenon that Higher output is not accompanied by a higher income.Although farmyard manure can be with
Overcome the problems, such as above section, but farmyard manure is easy to be not thorough because of heap fermentation, causes the biography of pathogenic microorganism and helminth (ovum)
The problems such as broadcasting causes environment.Therefore, searching can replace or cooperate with conventional composite fertilizer, and there is farmyard manure to improve soil
The novel organic fertilizer of effect is very necessary.
Seaweed bio it is organic it is fat polymerize various active substance in ocean, algal polysaccharides and oligosaccharide rich in,
Seeweed polyphenol, fucoidin, fucoidin, mannitol, Iodine, protein, glycine betaine, natural plants growth regulator, with
And peptide abundant and Aminosteril KE, nutrition comprehensive and abundant, it is natural soil conditioner, while there is disease-resistant, drought resisting, anti-
Cold, moisturizing promotes photosynthesis, growth promotion, promotes crop products flavor, improves the various actives functions such as crop quality.Its
Nutritional ingredient all derives from plant, not only can directly be absorbed by crop, reduces energy consumption, and will not cause completely to environment
Any pollution is the good fertilizer for really meeting modern agricultural development direction.Seaweed bio organic fertilizer is to corn, rice, big simultaneously
The cereal crops such as wheat, wheat, soybean, pachyrhizus, potato, peanut, pea, cucumber, tomato, eggplant, Chinese cabbage, ginger, watermelon, celery
The fruit trees such as the vegetable crops such as dish, shallot, garlic and apple, pears, peach, persimmon, citrus, banana, mango, grape, Chinese date, especially
There are bigger effect of increasing production and economic benefit to the industrial crops such as cotton, tobacco, mulberry tea and horticulture.
Summary of the invention
The object of the present invention is to provide a kind of methods for producing seaweed total nutrient liquid bio-organic fertilizer.
Technical solution of the present invention foundation:
1, the seaweed raw material in the present invention extracts in the brown alga at Atlantic Ocean deep-sea, and seaweed active constituent is high, includes seaweed
Polysaccharide, phenols poly-compounds, mannitol, glycine betaine, plant growth regulator, polypeptide, oligopeptides, free amino acid etc., can mention
High crop quality increases stress resistance of plant, improves soil.
2, the present invention improves the bioactivity and production of algal polysaccharides through biological enzyme in such a way that choline formulation combines
Amount.
3, the present invention enriches the type of trehalose, the oligosaccharides of generation has good by biological enzyme degradation part algal polysaccharides
Good resistance.
4, the present invention forms it into seaweed peptide and free amino acid, pole by combined-enzyme method hydrolyzable moiety seaweed protein
The big substance classes for enriching seaweed organic fertilizer, and there is abundant action function.
5, the present invention is added to bacillus subtilis in seaweed organic fertilizer, by the metabolite in its life cycle,
Active organic matter in abundant fertilizer.
Specific implementation method
1, dry kelp is crushed to 200 mesh with pulverizer.
2, smashed kelp is pressed to the proportion and water mixed injection reaction kettle of solid-liquid ratio 1:30.
3, the pH value of seaweed solution is modulated to 5 with phosphoric acid.
4,1% papain of overall solution volume, 1% pectase, 1% zytase, 0.5% fiber are separately added into
Fibroin enzyme, 30 DEG C at a temperature of carry out enzymatic hydrolysis 3 hours after, high temperature enzyme deactivation.
5, potassium hydroxide is added according to the 1.5% of solution total amount, and the seaweed solution temperature in reaction kettle is modulated 75 DEG C,
It is digested.Digestion time is 1.5 hours.
6, after digesting, the pH value of digestive juice is modulated 5.0 with phosphoric acid, the case where temperature of reaction kettle is 30 degrees Celsius
Lower addition algal polysaccharides degrading enzyme, additional amount are the 0.5% of solution total amount, and the reaction time is three hours.
7, potassium hydroxide is added and solution pH value is modulated 7.0, dry Kelp Powder weight 0.4% is added into reaction kettle respectively
The alkali protease of flavor protease and dry Kelp Powder weight 0.4%, reaction kettle are warming up to 55 DEG C, after digesting four hours, go out
Enzyme.(note: wind pepsin and alkali protease can be replaced with other restriction endonucleases and excision enzyme, and living according to the pH value of protease
Property section and preference temperature section adjustment pH value and reaction temperature.)
8, solution is filtered, the liquid bacillus subtilis bacterium solution of kelp dry powder weight 5% is added into filtered solution, stirs
Mixing is mixed, static 24 hours, a kind of seaweed total nutrient liquid bio-organic fertilizer can be obtained.
Claims (1)
1. a kind of preparation method of seaweed total nutrient liquid bio-organic fertilizer, it is characterised in that follow the steps below:
By the Kelp Powder of crushing in solid-liquid ratio 1:30 ratio inject reaction kettle, the pH value of seaweed solution is modulated to 5, respectively plus
Enter 1% papain of solution totality, 1% pectase, 1% zytase, 0.5% cellulose protease, in 30 DEG C of temperature
After carrying out 3 hours of enzymatic hydrolysis under degree, potassium hydroxide is added according to the 1.5% of solution total amount in high temperature enzyme deactivation, and will be in reaction kettle
Seaweed solution temperature modulates 75 DEG C, is digested, after digestion, dry Kelp Powder weight 0.4% is added into reaction kettle respectively
The alkali protease of flavor protease and dry Kelp Powder weight 0.4%, enzyme deactivation after enzymatic hydrolysis, solution is filtered, to filtered solution
The middle liquid bacillus subtilis bacterium solution that kelp dry powder weight 5% is added.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910681144.0A CN110372443A (en) | 2019-07-26 | 2019-07-26 | A kind of preparation method of the bio-organic fertilizer of total nutrient liquid containing seaweed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910681144.0A CN110372443A (en) | 2019-07-26 | 2019-07-26 | A kind of preparation method of the bio-organic fertilizer of total nutrient liquid containing seaweed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110372443A true CN110372443A (en) | 2019-10-25 |
Family
ID=68256261
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910681144.0A Pending CN110372443A (en) | 2019-07-26 | 2019-07-26 | A kind of preparation method of the bio-organic fertilizer of total nutrient liquid containing seaweed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110372443A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114380642A (en) * | 2022-01-29 | 2022-04-22 | 哈尔滨皮加诺生物科技有限公司 | Microbial agent composition and application thereof in crop cultivation |
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| CN106495827A (en) * | 2016-09-29 | 2017-03-15 | 五洲丰农业科技有限公司 | The method that alkali carries and classification enzymolysis joint prepare the organic liquid fertilizer of ocean containing oligosaccharide |
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| CN108484314A (en) * | 2018-03-19 | 2018-09-04 | 辽宁省农业科学院 | It is a kind of promote hickory chick growth microorganism liquid seaweed extract and its application |
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| CN108935523A (en) * | 2018-08-05 | 2018-12-07 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of preparation method of marine algae extract |
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| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
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|---|---|---|---|---|
| CN114380642A (en) * | 2022-01-29 | 2022-04-22 | 哈尔滨皮加诺生物科技有限公司 | Microbial agent composition and application thereof in crop cultivation |
| CN114380642B (en) * | 2022-01-29 | 2022-09-20 | 哈尔滨皮加诺生物科技有限公司 | Microbial agent composition and application thereof in crop cultivation |
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Application publication date: 20191025 |